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Health-related quality of life in Chinese population with non-alcoholic fatty liver disease: a national multicenter survey.

Health Qual Life Outcomes 2021 May 7;19(1):140. Epub 2021 May 7.

Hepatopancreatobiliary Center, Beijing Tsinghua Changgung Hospital, Tsinghua University, No.168, Litang Road, Changping District, Beijing, 102218, China.

Background: Health Related Quality of Life (HRQL) is a multi-dimensional construct that can comprehensively evaluate the patient's health status, including physical, emotional, mental and social well-being. In this study, we aimed to evaluate the impact of non-alcoholic fatty liver disease (NAFLD) on HRQL in a Chinese population.

Methods: In this national multicenter cross-sectional survey, patients with NAFLD were enrolled. Read More

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Influence of sensor mass and adipose tissue on the mechanomyography signal of elbow flexor muscles.

J Biomech 2021 Apr 21;122:110456. Epub 2021 Apr 21.

Graduate Program in Health Technology, Pontifícia Universidade Católica do Paraná (PPGTS/PUCPR), Paraná, Brazil; Graduate Program in Electrical and Computer Engineering, Federal Technological University of Paraná UTFPR - Curitiba-Paraná/ Brazil. Electronic address:

Mechanomyography (MMG) is a non-invasive technique that records muscle contraction using sensors positioned on the skin's surface. Therefore, it can have its signal attenuated due to the adipose tissue, directly influencing the results. This study evaluates the influence of different mass added to a sensor's assembly and the adipose tissue on MMG signals of elbow flexor muscles. Read More

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Structure-based design approach of potential BCL-2 inhibitors for cancer chemotherapy.

Comput Biol Med 2021 Apr 30;134:104455. Epub 2021 Apr 30.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, M.S. Ramaiah University of Applied Sciences, Bengaluru, 560054, Karnataka, India. Electronic address:

B-cell lymphoma 2 (BCL-2) family is one of the chief regulators of cellular apoptosis. The intricate interactions between pro-apoptotic and anti-apoptotic genes of the BCL-2 family dictate the apoptotic balance of the cell. An overexpression of the anti-apoptotic members of BCL-2 is indicative of cell death evasion and cancer metastasis. Read More

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Variance in expression and localization of sex-related genes CgDsx, CgBHMG1 and CgFoxl2 during diploid and triploid Pacific oyster Crassostrea gigas gonad differentiation.

Gene 2021 May 4:145692. Epub 2021 May 4.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.

Several evolutionarily conserved classes of transcriptional regulators were involved in diverse sex determination and differentiation pathways across taxa, whereas their roles in most mollusks is still limited. The Pacific oyster Crassostrea gigas, a dioecious bivalve with sex reversal, could be an ideal model for this issue because of its complex sexuality and potential disruption of sex differentiation in triploid individuals. Here, two mRNA splicing isoforms of a DM domain gene CgDsx and two isoforms of a novel sex-related CgBHMG1 (ortholog of BHMG1 in mammals) were identified in C. Read More

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Characterization of Litopenaeus vannamei secreted protein acidic and rich in cysteine -like in WSSV infection.

J Invertebr Pathol 2021 May 4:107593. Epub 2021 May 4.

Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology; Shanghai Ocean University, Shanghai 201306.

Secreted protein acidic and rich in cysteine (SPARC) is an extracellular and non-structural glycoprotein. In shrimp, a significant function of SPARC in WSSV infection remains unclear. In this study, the full-length cDNA sequence of a secreted protein acidic and rich in cysteine -like was cloned from shrimp Litopenaeus vannamei (named as LvSPARC-L). Read More

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Genome-wide identification of ABCC gene family and their expression analysis in pigment deposition of fiber in brown cotton (Gossypium hirsutum).

PLoS One 2021 7;16(5):e0246649. Epub 2021 May 7.

School of Life Sciences, Anhui Agricultural University, Hefei, PR China.

ABC (ATP-binding cassette) transporters are a class of superfamily transmembrane proteins that are commonly observed in natural organisms. The ABCC (ATP-binding cassette C subfamily) protein belongs to a subfamily of the ABC protein family and is a multidrug resistance-associated transporter that localizes to the tonoplast and plays a significant role in pathogenic microbial responses, heavy metal regulation, secondary metabolite transport, and plant growth. Recent studies have shown that the ABCC protein is also involved in the transport of anthocyanins/proanthocyanidins (PAs). Read More

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RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein.

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Psoriasis is a chronic, inflammatory skin disease with a high incidence and recurrence; however, its exact pathogenesis and aetiology remain unclear. This study aimed to analyse the effect of the upstream negative regulator RAS-association domain family 1A (RASSF1A) on Yes-associated protein (YAP) in psoriasis. Skin lesions of 22 patients with psoriasis and 19 healthy controls were used. Read More

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Ability of neural network cells in learning teacher motivation scale and prediction of motivation with fuzzy logic system.

Sci Rep 2021 May 6;11(1):9721. Epub 2021 May 6.

Laboratory of Computational Modeling of Drugs, South Ural State University, 76 Lenin Prospekt, 454080, Chelyabinsk, Russia.

We employed a new approach in the field of social sciences or psychological aspects of teaching besides using a very common software package that is Statistical Package for the Social Sciences (SPSS). Artificial intelligence (AI) is a new domain that the methods of its data analysis could provide the researchers with new insights for their research studies and more innovative ways to analyze their data or verify the data with this method. Also, a very significant element in teaching is teacher motivation that is the trigger that pushes the teachers forward, depending on some internal and external factors. Read More

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Structural and mechanistic insights into the bifunctional HISN2 enzyme catalyzing the second and third steps of histidine biosynthesis in plants.

Sci Rep 2021 May 6;11(1):9647. Epub 2021 May 6.

Center for Biocrystallographic Research, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.

The second and third steps of the histidine biosynthetic pathway (HBP) in plants are catalyzed by a bifunctional enzyme-HISN2. The enzyme consists of two distinct domains, active respectively as a phosphoribosyl-AMP cyclohydrolase (PRA-CH) and phosphoribosyl-ATP pyrophosphatase (PRA-PH). The domains are analogous to single-domain enzymes encoded by bacterial hisI and hisE genes, respectively. Read More

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evidences on filarial cystatin as a putative ligand of human TLR4.

J Biomol Struct Dyn 2021 May 6:1-17. Epub 2021 May 6.

Integrative Biochemistry & Immunology Laboratory, Department of Animal Science, Kazi Nazrul University, Asansol, West Bengal, India.

Cystatin is a small molecular weight immunomodulatory protein of filarial parasite that plays a pivotal role in downregulating the host immune response to prolong the survival of the parasite inside the host body. Hitherto, this protein is familiar as an inhibitor of human proteases. However, growing evidences on the role of cystatin in regulating inflammatory homeostasis prompted us to investigate the molecular reasons behind the explicit anti-inflammatory trait of this protein. Read More

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Molecular Characterization of Complement Component 3 (C3) in the Pearl Oyster Improves Our Understanding of the Primitive Complement System in Bivalve.

Front Immunol 2021 19;12:652805. Epub 2021 Apr 19.

College of Fisheries, Guangdong Ocean University, Zhanjiang, China.

As the central component in the complement system, complement component 3 (C3) plays essential roles in both the innate and adaptive immune responses. Here, a C3 gene (designated as ) was obtained from the pearl oyster by RT-PCR and rapid amplification of cDNA ends (RACE). The cDNA consists of 5,634 bp with an open reading frame (ORF) of 5,193 bp encoding a protein of 1,730 amino acids with a 19 residue signal peptide. Read More

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Unconventional p97/VCP-Mediated ER to Endosomal Trafficking of a Retroviral Protein.

J Virol 2021 May 5. Epub 2021 May 5.

Department of Molecular Biosciences, Center for Infectious Disease, and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX

Mouse mammary tumor virus (MMTV) encodes a Rem precursor protein that specifies both regulatory and accessory functions. Rem is cleaved at the ER membrane into a functional N-terminal signal peptide (SP) and the C-terminus (Rem-CT). Rem-CT lacks a membrane-spanning domain and a known ER retention signal, yet was not detectably secreted into cell supernatants. Read More

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Agglutination Activity of Fasciola gigantica DM9-1, a Mannose-Binding Lectin.

Korean J Parasitol 2021 Apr 22;59(2):173-178. Epub 2021 Apr 22.

Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani 12121, Thailand.

The DM9 domain is a protein unit of 60-75 amino acids that has been first detected in the fruit fly Drosophila as a repeated motif of unknown function. Recent research on proteins carrying DM9 domains in the mosquito Anopheles gambiae and the oyster Crassostrea gigas indicated an association with the uptake of microbial organisms. Likewise, in the trematode Fasciola gigantica DM9-1 showed intracellular relocalization following microbial, heat and drug stress. Read More

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The Beta vulgaris-derived resistance gene Rz2 confers broad-spectrum resistance against soilborne sugar beet-infecting viruses from different families by recognizing triple gene block protein 1.

Mol Plant Pathol 2021 May 5. Epub 2021 May 5.

Institute of Sugar Beet Research, Göttingen, Germany.

Sugar beet cultivation is dependent on an effective control of beet necrotic yellow vein virus (BNYVV, family Benyviridae), which causes tremendous economic losses in sugar production. As the virus is transmitted by a soilborne protist, the use of resistant cultivars is currently the only way to control the disease. The Rz2 gene product belongs to a family of proteins conferring resistance towards diverse pathogens in plants. Read More

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The role of E255K/V-inclusive mutations in a Philadelphia-positive acute lymphoblastic leukemia with mutation evolution during sequential TKIs therapies: A case report.

Medicine (Baltimore) 2021 May;100(18):e25579

Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan.

Rationale: Until recently, the survival rate in patients with Philadelphia-positive acute lymphoblastic leukemia (Ph+ ALL) was approximately 30%. Tyrosine kinase inhibitors (TKIs), which are a new class of drugs that target BCR-ABL fusion protein, have shown to be effective in treating Ph+ ALL in adults. However, the resistance mechanisms that promote the disease recurrence have altered the initial success of these revolutionary agents. Read More

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TM2D1 contributes the epithelial-mesenchymal transition of hepatocellular carcinoma via modulating AKT/β-catenin axis.

Am J Cancer Res 2021 15;11(4):1557-1571. Epub 2021 Apr 15.

Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University Shanghai 200032, P. R. China.

Various epidemiology studies showed the correlation between Alzheimer's disease (AD) and low incidence of cancer. However, the etiology underlying etiology of AD-related carcinogenesis remains largely elusive. Our study focused on characterizing the role of TM2D1 (TM2 domain containing 1) in hepatocellular carcinoma. Read More

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The DIAMOND portal: An innovative platform for sharing translational research workforce training and assessment resources.

J Clin Transl Sci 2020 Jul 6;4(6):480-484. Epub 2020 Jul 6.

Michigan Institute for Clinical and Health Research, University of Michigan, Ann Arbor, MI, USA.

Although several initiatives have produced core competency domains for training the translational science workforce, training resources to help clinical research professionals advance these skills reside primarily within local departments or institutions. The Development, Implementation, and AssessMent of Novel Training in Domain (DIAMOND) project was designed to make this training more readily and publicly available. DIAMOND includes a digital portal to catalog publicly available educational resources and an ePortfolio to document professional development. Read More

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Proteomic and phosphoproteomic profiles of Sertoli cells in buffalo.

Theriogenology 2021 Apr 27;170:1-14. Epub 2021 Apr 27.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China. Electronic address:

Sertoli cells provide nutrients and support for germ cell differentiation and maintain a stable microenvironment for spermatogenesis. Comprehensive identification of Sertoli cellular proteins is important in understanding spermatogenesis. In this study, we performed an integrative analysis of the proteome and phosphoproteome to explore the role of Sertoli cells in spermatogenesis. Read More

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Ageing and glaucoma progression of the retinal nerve fibre layer using spectral-domain optical coherence tomography analysis.

Acta Ophthalmol 2021 May 9;99(3):260-268. Epub 2020 Aug 9.

Ophthalmology, Department of Clinical Sciences in Malmö, Lund University, Malmö, Sweden.

Purpose: To compare the effects of ageing and glaucoma progression on the thickness of the circumpapillary retinal nerve fibre layer (cpRNFL) and to evaluate the performance of a set of optical coherence tomography (OCT) progression analyses.

Methods: The cpRNFL was measured twice by OCT at each of two visits made 10 years apart in 69 healthy individuals and 49 glaucoma patients. Both visits also included Humphrey 24-2 SITA standard testing. Read More

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A novel essential splice site variant in SPTB in a large hereditary spherocytosis family.

Mol Genet Genomic Med 2021 May 4:e1641. Epub 2021 May 4.

Department of Cancer Biology and Genetics, The Ohio State University, Columbus, Ohio, USA.

Background: We studied a large family with 22 individuals affected with autosomal dominant hereditary spherocytosis (HS).

Methods: Genome-wide linkage, whole-genome sequencing (WGS), Sanger sequencing, RT-PCR, and ToPO TA cloning analyses were performed.

Results: We revealed a heterozygous G>A transition in the 14q23 locus, at position +1 of the intron 8 donor splice site of the spectrin beta, erythrocytic (SPTB) gene. Read More

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Clinical and molecular genetic analysis of a Chinese family with hereditary elliptocytosis caused by a novel mutation in the EPB41 gene.

J Clin Lab Anal 2021 May 4:e23781. Epub 2021 May 4.

Department of Laboratory Medicine, the First Affiliated Hospital of Shantou University Medical College, Shantou, P.R. China.

Background: Hereditary elliptocytosis (HE) is a heterogeneous red blood cell membrane disorder characterized by the presence of elliptocytes on a peripheral blood smear. Clinical manifestations of HE vary widely from asymptomatic carriers to patients with severe transfusion-dependent anemia. Most patients are asymptomatic or have mild anemia, which hinders diagnosis. Read More

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Phosphorylation-dependent subfunctionalization of the calcium-dependent protein kinase CPK28.

Proc Natl Acad Sci U S A 2021 May;118(19)

Department of Biology, Queen's University, Kingston, ON K7L 3N6, Canada;

Calcium (Ca)-dependent protein kinases (CDPKs or CPKs) are a unique family of Ca sensor/kinase-effector proteins with diverse functions in plants. In , CPK28 contributes to immune homeostasis by promoting degradation of the key immune signaling receptor-like cytoplasmic kinase BOTRYTIS-INDUCED KINASE 1 (BIK1) and additionally functions in vegetative-to-reproductive stage transition. How CPK28 controls these seemingly disparate pathways is unknown. Read More

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ToxR mediates the antivirulence activity of phenyl-arginine-β-naphthylamide to attenuate virulence.

Infect Immun 2021 May 3. Epub 2021 May 3.

University of Pittsburgh School of Medicine, Department of Microbiology and Molecular Genetics Pittsburgh, PA

Multidrug efflux systems belonging to the resistance-nodulation-cell division (RND) family are ubiquitous in Gram negative bacteria and critical for antimicrobial resistance. This realization has led to efforts to develop efflux pump inhibitors (EPI) for use as adjuvants for antibiotic treatment of resistant organisms. However, the functions of RND transporters extend beyond antimicrobial resistance to include physiological functions that are critical for pathogenesis, suggesting that EPIs could also be used as antivirulence therapeutics. Read More

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Carbonic Anhydrase in Pacific Abalone : Characterization, Expression, and Role in Biomineralization.

Front Mol Biosci 2021 15;8:655115. Epub 2021 Apr 15.

Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, Yeosu, South Korea.

Carbonic anhydrases (CAs) are universal zinc ion containing metalloenzymes that play a pivotal role in various physiological processes. In this study, a CA I (designated as Hdh CA I) was isolated and characterized from the mantle tissue of Pacific abalone, . The full-length cDNA sequence of Hdh CA I was 1,417-bp in length, encoding a protein of 337 amino acids with molecular weight of 37. Read More

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PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway.

Exp Ther Med 2021 Jun 14;21(6):613. Epub 2021 Apr 14.

Department of General Surgery, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China.

Endoplasmic reticulum stress (ERS)-induced apoptosis serves a crucial role in the pathogenesis of myocardial ischemia/reperfusion injury (MIRI). Previous studies have confirmed that pleckstrin homology-like domain family A member 3 (PHLDA3) is an important mediator in ERS-associated apoptosis. The aim of the current study focused on whether PHLDA3 served protective effects on hypoxia/reoxygenation (H/R)-injured cardiomyocytes by inhibiting ERS-induced apoptosis. Read More

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Exosomes from LPS-Stimulated hDPSCs Activated the Angiogenic Potential of HUVECs In Vitro.

Stem Cells Int 2021 15;2021:6685307. Epub 2021 Apr 15.

Department of Stomatology, Nanfang Hospital, Southern Medical University, School of Stomatology, 1838 Guangzhou Avenue North, Guangzhou 510515, China.

Background: Exosomes from human dental pulp stem cells (hDPSCs) were indicated to play a positive role in vascular regeneration processes. But the angiogenic capabilities of exosomes from inflammatory hDPSCs and the underlying mechanism remain unknown. In this study, the inflammatory factor lipopolysaccharide (LPS) was used to stimulate hDPSCs, and exosomes were extracted from these hDPSCs. Read More

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Is Relating With M2 Macrophage and Remarkable Malignancy Characters in Low-Grade Glioma.

Front Immunol 2021 16;12:659659. Epub 2021 Apr 16.

Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.

Immunoreactions regulated by TAMs (Tumor-associated macrophages) play a pivotal role in tumorigenesis and metastasis. In recent decades, treatments based on immune regulation have achieved revolutionary breakthroughs in cancer targeted therapies. The phenotypes of TAMs in gliomas are more heterogeneous and inherently complex than can be simply defined by classification into the M1 and M2 polarized states. Read More

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Preparing student teachers for classrooms through experiences of critical incidents during remote practicum: A meaningful-experiential learning perspective.

Educ Inf Technol (Dordr) 2021 Apr 23:1-23. Epub 2021 Apr 23.

Department of English Language Teaching, Necmettin Erbakan University, Konya, Turkey.

The Covid-19 pandemic has precipitated dramatic changes in education systems around the world and pre-service teacher preparation programs have been notably impacted by the associated difficulties and constraints. Due to lockdown of universities and schools, practicum placements had to shift from in-person to virtual learning environments. However, being a practical classroom activity, practicum posed particular challenges that prompted teacher educators to make adjustments in their pedagogical strategies to meet the course requirements. Read More

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The natural antisense transcript derived from the lncRNA locus interacts with SET domain protein ASHR3 during inception of symbiosis in Arachis hypogaea.

Mol Plant Microbe Interact 2021 May 2. Epub 2021 May 2.

University of Calcutta, 30163, Biochemistry, Kolkata, West Bengal, India;

The lncRNA is required for cortical cell division during root nodule symbiosis (RNS) of legumes, though it is not essential for actinorhizal RNS. Our objective was to understand whether was required for aeschynomenoid nodule formation in . express from chr5 () and chr15 () during symbiosis, and RNA interference of these transcripts drastically affected nodulation indicating the importance of in . Read More

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Functional characterization of four TIR domain-containing adaptors, MyD88, TRIF, MAL, and SARM in mandarin fish Siniperca chuatsi.

Dev Comp Immunol 2021 Apr 29;122:104110. Epub 2021 Apr 29.

State Key Laboratory of Freshwater Ecology and Biotechnology, And Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, Shandong Province, 266237, China; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China. Electronic address:

Toll/interleukin-1 receptor (TIR) domain-containing adaptors, serve as pivotal signal transduction molecules in Toll-like receptor (TLR) signalling pathway to mediate downstream signalling cascades. In this study, four TIR-domain containing adaptors, MyD88, TRIF, MAL and SARM, were identified in mandarin fish Siniperca chuatsi, and they all contain TIR domains, of which MyD88 and SARM had high sequence homology with their vertebrate homologues. The expression analysis at mRNA level indicated that these genes were ubiquitously distributed in different tissues, being high in immune- and mucosa-related tissues such as head-kidney and intestine. Read More

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