23,955 results match your criteria dna minor

Maternal posttraumatic stress and FKBP5 Genotype interact to predict trauma-related symptoms in preschool-age offspring.

J Affect Disord 2021 May 26;292:212-216. Epub 2021 May 26.

Department of Psychiatry, University of Connecticut School of Medicine, Farmington, CT, United States. Electronic address:

Background: Children of parents with posttraumatic stress (PTS) face heightened risk for developing emotional and behavioral problems, regardless of whether they experience a traumatic event themselves. The current study investigates whether child FKBP5, a stress relevant gene shown to interact with child trauma exposure to increase risk for PTS, also moderates the well-established link between maternal PTS and child symptoms.

Methods: Data are derived from a longitudinal lab-based study for which 205 dyads of trauma-exposed mothers and their preschool-age children from a sample enriched for violence exposure provided DNA samples and completed measures of maternal and child trauma-related symptoms. Read More

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Paternal MTHFR deficiency leads to hypomethylation of young retrotransposons and reproductive decline across two successive generations.

Development 2021 Jun 15. Epub 2021 Jun 15.

Department of Human Genetics, McGill University, Montreal, QC, Canada.

5, 10-Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in the folate metabolic pathway with a key role in generating methyl groups. As MTHFR deficiency impacts male fertility and sperm DNA methylation, there is the potential for epimutations to be passed to the next generation. Here, we assessed whether the impact of MTHFR deficiency on testis morphology and sperm DNA methylation is exacerbated across generations. Read More

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Macrodiolide Diversification Reveals Broad Immunosuppressive Activity That Impairs the cGAS-STING Pathway.

Angew Chem Int Ed Engl 2021 Jun 14. Epub 2021 Jun 14.

Aarhus University, Chemistry, Langelandsgade 140, 8000, Aarhus C, DENMARK.

The development of new immunomodulatory agents can impact various areas of medicine. In particular, compounds with the ability to modulate innate immunological pathways hold significant unexplored potential. Here, we report a modular synthetic approach to the macrodiolide natural product (-)-vermiculine, an agent previously shown to possess diverse biological effects including cytotoxic and immunosuppressive activity. Read More

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Redefining genera of cereal pathogens: , and .

Fungal Syst Evol 2021 Jun 7;7:67-98. Epub 2020 Dec 7.

Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.

The taxonomy of and is re-evaluated, along with that of phylogenetically related genera Isolates are identified using comparisons of DNA sequences of the internal transcribed spacer ribosomal RNA locus (ITS), partial translation elongation factor 1-alpha (), actin (), DNA-directed RNA polymerase II largest () and second largest subunit () genes, and the nuclear ribosomal large subunit (LSU), combined with their morphological characteristics. is restricted to two species, and , with placed in , and accommodated in a new genus, . . Read More

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Spectroscopic and Simulation Studies of the Sequence-Dependent DNA Destabilization by a Fungicide.

ACS Omega 2021 Jun 26;6(22):14371-14378. Epub 2021 May 26.

School of Chemical Sciences, Indian Association for the Cultivation of Science, Kolkata 700032, India.

The understanding of the structural change of DNA induced by fungicides is essential as the non-targeted action of fungicides causes genotoxicity, leading to several serious diseases such as cancer, behavioral change, and nausea. In this study, the binding of an important fungicide, namely, -dodecylguanidine acetate (dodine), with B-DNA having different sequences of nucleobases and its effect on the structure of B-DNA has been investigated using spectroscopic and simulation methods. In general, the addition of dodine destabilizes DNA; however, the binding of dodine causing the destabilization of DNA is highly sequence dependent. Read More

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A comparison on the biochemical activities of Fluorescein disodium, Rose Bengal and Rhodamine 101 in the light of DNA binding, antimicrobial and cytotoxic study.

J Biomol Struct Dyn 2021 Jun 14:1-12. Epub 2021 Jun 14.

Department of Chemistry, Aliah University, Kolkata, India.

Biochemical activities of Fluorescein, Rose Bengal and Rhodamine 101 were studied by DNA binding, antibacterial and cytotoxic studies. DNA binding studies were done using spectroscopic, thermodynamic and molecular modeling techniques. Antibacterial activities were investigated against a gram-negative bacteria and a gram-positive bacteria . Read More

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Feasibility of Social Media Recruitment for Orofacial Cleft Genetic Research.

Cleft Palate Craniofac J 2021 Jun 14:10556656211024484. Epub 2021 Jun 14.

1371Emory University, Atlanta, GA, USA.

Objective: This study assessed the feasibility of unpaid social media advertising to recruit participants affected with an orofacial cleft (OFC) for a genetic study.

Design: This is a retrospective analysis of recruitment based on enrollment and participation in a genetic study. Participants completed a series of enrollment surveys, provided saliva samples, and completed postparticipation feedback surveys. Read More

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Molecular genetic diversity and population structure analyses of rutabaga accessions from Nordic countries as revealed by single nucleotide polymorphism markers.

BMC Genomics 2021 Jun 12;22(1):442. Epub 2021 Jun 12.

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.

Background: Rutabaga or swede (Brassica napus ssp. napobrassica (L.) Hanelt) varies in root and leaf shape and colour, flesh colour, foliage growth habits, maturity date, seed quality parameters, disease resistance and other traits. Read More

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A Novel Minor Groove Binder as a Potential Therapeutic Agent for Myotonic Dystrophy Type 1.

ChemMedChem 2021 Jun 10. Epub 2021 Jun 10.

Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S Mathews Avenue, 61801, Urbana, IL, USA.

Myotonic dystrophy type 1 (DM1) is a multisystemic neuromuscular disorder that is inherited in an autosomal dominant manner. DM1 originates in a (CTG⋅CAG) repeat expansion in the 3'-UTR of the dystrophia myotonic protein kinase (DMPK) gene on chromosome 19. One of the transcripts, r(CUG) , is toxic in various ways. Read More

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Analogous telomeres shortening and different metabolic profile: hypertension versus hypertension/type 2 diabetes mellitus comorbidity.

Cardiovasc Endocrinol Metab 2021 Jun 3;10(2):106-112. Epub 2020 Sep 3.

Department of Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University.

Background: Eukaryotes chromosomal ends are capped and protected by telomeres, which are noncoding DNA repeats synthesized by telomerase enzyme. The telomerase enzyme is a nucleoprotein encoded by and genes. Naturally, the length of the telomeres shortens with each cell cycle but the shortening is fastened in certain age-related diseases like hypertension (HTN) and type 2 diabetes mellitus (T2DM). Read More

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Post-Biopsy Cell-Free DNA From Blood: An Open Window on Primary Prostate Cancer Genetics and Biology.

Front Oncol 2021 24;11:654140. Epub 2021 May 24.

Department of Biomedical Sciences, Humanitas University, Milan, Italy.

Circulating cell-free DNA (ccfDNA), released from normal and cancerous cells, is a promising biomarker for cancer detection as in neoplastic patients it is enriched in tumor-derived DNA (ctDNA). ctDNA contains cancer-specific mutations and epigenetic modifications, which can have diagnostic/prognostic value. However, in primary tumors, and in particular in localized prostate cancer (PCa), the fraction of ctDNA is very low and conventional strategies to study ccfDNA are unsuccessful. Read More

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SNP detection and population structure evaluation of Y. L. Chang et Skv. in Hunshandake Sandland, Inner Mongolia, China.

Physiol Mol Biol Plants 2021 May 24;27(5):997-1005. Epub 2021 Apr 24.

Faculty of Resources and Environment, Baotou Teachers' College, Inner Mongolia University of Science and Technology, Baotou, 014030 China.

Single nucleotide polymorphisms (SNPs) are the most abundant and richest form of genomic polymorphism and, hence, are highly favorable markers for genetic map construction and genome-wide association studies. Based on the DNA specific-locus amplified fragment sequencing (SLAF-seq) for large-scale SNP detection, the genetic diversity and population structure of Y. L. Read More

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Quantitation of Trace Levels of DNA Released from Disrupted Adeno-Associated Virus Gene Therapy Vectors.

J Pharm Sci 2021 Jun 6. Epub 2021 Jun 6.

Formulation and Drug Product Development, REGENXBIO Inc., Rockville, MD 20850, USA.

Adeno-associated virus (AAV) vectors for gene therapy have potential to provide a durable treatment response for a number of diseases with unmet need. DNA is released from AAV capsids at high temperatures. Less is known about DNA release that may occur under conditions relevant to clinical and commercial manufacturing, storage, and distribution. Read More

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Clostridioides difficile specific DNA adenine methyltransferase CamA squeezes and flips adenine out of DNA helix.

Nat Commun 2021 06 8;12(1):3436. Epub 2021 Jun 8.

Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Clostridioides difficile infections are an urgent medical problem. The newly discovered C. difficile adenine methyltransferase A (CamA) is specified by all C. Read More

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Deep learning connects DNA traces to transcription to reveal predictive features beyond enhancer-promoter contact.

Nat Commun 2021 06 8;12(1):3423. Epub 2021 Jun 8.

Department of Developmental Biology, Stanford University, Stanford, CA, USA.

Chromatin architecture plays an important role in gene regulation. Recent advances in super-resolution microscopy have made it possible to measure chromatin 3D structure and transcription in thousands of single cells. However, leveraging these complex data sets with a computationally unbiased method has been challenging. Read More

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Transmission of light signals from the light-oxygen-voltage core via the hydrophobic region of the β-sheet surface in aureochrome-1.

Sci Rep 2021 Jun 7;11(1):11995. Epub 2021 Jun 7.

Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan.

Light-Oxygen-Voltage (LOV) domains are responsible for detecting blue light (BL) and regulating the activities of effector domains in various organisms. Photozipper (PZ), an N-terminally truncated aureochrome-1 protein, contains a LOV domain and a basic leucin zipper (bZIP) domain and plays a role as a light-activatable transcription factor. PZ is monomeric in the dark state and undergoes non-covalent dimerization upon illumination with BL, subsequently increasing its affinity for the target DNA. Read More

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Polymorphic variants of alkaline phosphatase gene correlate with clinical signs of adult hypophosphatasia?

Osteoporos Int 2021 Jun 7. Epub 2021 Jun 7.

Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy.

We analyzed polymorphism of the ALPL gene in patients with low serum levels of tissue-nonspecific alkaline phosphatase (TNAP). The presence of three or more of the less frequent alleles of ALPL polymorphisms was associated with significantly lower TNAP serum level and higher frequencies of metatarsal fractures, which may help confirm a clinical suspicion of adult hypophosphatasia.

Introduction: Alkaline phosphatases (ALPs) are membrane-bound enzymes that hydrolyze monophosphate esters at a high pH (pH 8-10). Read More

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Synthesis, DNA-Binding, Anticancer Evaluation, and Molecular Docking Studies of Bishomoleptic and Trisheteroleptic Ru-Diimine Complexes Bearing 2-(2-Pyridyl)-quinoxaline.

Bioinorg Chem Appl 2021 12;2021:5599773. Epub 2021 May 12.

Inorganic Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou 157 71, Greece.

Herein, we report the synthesis and characterization of a bishomoleptic and a trisheteroleptic ruthenium (II) polypyridyl complex, namely, [Ru(bpy)2(2, 2'-pq)](PF6)2 (1) and [Ru(bpy) (phen) (2, 2'-pq)](PF6)2 (2), respectively, where bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline, and 2, 2'-pq = 2-(2'-pyridyl)-quinoxaline. The complexes were characterized by elemental analysis, TGA, H-NMR, FT-IR, UV-Vis, emission spectroscopy, and electrochemistry. Their structures were confirmed by single-crystal X-ray diffraction analysis. Read More

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Synthesis, in vitro antiprotozoal activity, molecular docking and molecular dynamics studies of some new monocationic guanidinobenzimidazoles.

Eur J Med Chem 2021 Oct 24;221:113545. Epub 2021 May 24.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ankara University, 06100, Tandogan, Ankara, Turkey. Electronic address:

A series of monocationic new guanidinobenzimidazole derivatives were prepared in a four step process starting from 2-nitro-1,4-phenylendiamine. Their antiparasitic activity against Plasmodium falciparum, Trypanosoma brucei rhodesiense, Trypanosoma cruzi and Leishmania donovani were evaluated in vitro. Two out of 20 tested monocationic compounds (7, 14) showed close activity with reference drug chloroquine against P. Read More

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October 2021

Occurrence of major and minor pathogens in calves diagnosed with bovine respiratory disease.

Vet Microbiol 2021 May 27;259:109135. Epub 2021 May 27.

Department of Veterinary and Animal Science University of Copenhagen, Copenhagen, Denmark. Electronic address:

Bovine respiratory disease (BRD) is caused by a mixture of viruses and opportunistic bacteria belonging to Pasteurellaceae and Mycoplasma bovis. However, these organisms are also commonly isolated from healthy calves. This study aimed to determine whether the organisms are present in higher numbers in calves sick with acute BRD than in clinically healthy calves, and further to genetically characterize bacteria of the family Pasteurellaceae to understand whether particular types are associated with disease. Read More

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Anti-melanoma effect of ruthenium(II)-diphosphine complexes containing naphthoquinone ligand.

J Inorg Biochem 2021 May 31;222:111497. Epub 2021 May 31.

Universidade de Franca, Avenida Dr. Armando Salles Oliveira, 201 - Parque Universitário, Franca, São Paulo 14404-600, Brazil. Electronic address:

The use of natural products as potential ligands has been explored as a strategy in the development of metal-based chemotherapy. Since ruthenium complexes are promising alternatives to traditional antitumor agents, this study evaluated the anti-melanoma potential of two ruthenium(II) complexes containing the naphthoquinone ligands lapachol (lap), [Ru(lap)(dppm)]PF, and lawsone (law), [Ru(law)(dppm)]PF, in addition to the bis(diphenylphosphino)methane (dppm) ligand, referred to as complexes (1) and (2), respectively, using a syngeneic murine melanoma model. Activation of the apoptotic pathway by the treatments was assessed by immunohistochemistry in tumor tissue. Read More

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Cell free protein synthesis versus yeast expression - A comparison using insulin as a model protein.

Protein Expr Purif 2021 Jun 2;186:105910. Epub 2021 Jun 2.

Novo Nordisk, A/S Novo Nordisk Park, DK-2760, Måløv, Denmark. Electronic address:

Expression of recombinant proteins traditionally require a cellular system to transcribe and translate foreign DNA to a desired protein. The process requires special knowledge of the specific cellular metabolism in use and is often time consuming and labour intensive. A cell free expression system provides an opportunity to express recombinant proteins without consideration of the living cell. Read More

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Integrating multiple chemical tracers to elucidate the diet and habitat of Cookiecutter Sharks.

Sci Rep 2021 Jun 3;11(1):11809. Epub 2021 Jun 3.

Monterey Bay Aquarium, Monterey, CA, USA.

The Cookiecutter shark (Isistius brasiliensis) is an ectoparasitic, mesopelagic shark that is known for removing plugs of tissue from larger prey, including teleosts, chondrichthyans, cephalopods, and marine mammals. Although this species is widely distributed throughout the world's tropical and subtropical oceanic waters, like many deep-water species, it remains very poorly understood due to its mesopelagic distribution. We used a suite of biochemical tracers, including stable isotope analysis (SIA), fatty acid analysis (FAA), and environmental DNA (eDNA), to investigate the trophic ecology of this species in the Central Pacific around Hawaii. Read More

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Genetic Variants of DNA Repair Genes as Predictors of Radiation-Induced Subcutaneous Fibrosis in Oropharyngeal Carcinoma.

Front Oncol 2021 17;11:652049. Epub 2021 May 17.

Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, India.

Purpose: To investigate the impact of genetic variants of DNA repair and pro-fibrotic pathway genes on the severity of radiation-induced subcutaneous fibrosis in patients of oropharyngeal carcinoma treated with radical radiotherapy.

Materials And Methods: Patients of newly diagnosed squamous cell carcinoma of oropharynx being treated with two-dimensional radical radiotherapy were enrolled in the study. Patients who had undergone surgery or were receiving concurrent chemotherapy were excluded. Read More

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Substrate deformation regulates DRM2-mediated DNA methylation in plants.

Sci Adv 2021 Jun 2;7(23). Epub 2021 Jun 2.

Department of Biochemistry, University of California, Riverside, Riverside, CA 92521, USA.

DNA methylation is a major epigenetic mechanism critical for gene expression and genome stability. In plants, domains rearranged methyltransferase 2 (DRM2) preferentially mediates CHH (H = C, T, or A) methylation, a substrate specificity distinct from that of mammalian DNA methyltransferases. However, the underlying mechanism is unknown. Read More

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A variant in is associated with decreased disease severity in COVID-19.

Meta Gene 2021 Sep 28;29:100930. Epub 2021 May 28.

Institute of Translational Research, Department of Genomics and Molecular Biology, Asian Institute of Gastroenterology, AIG Hospitals, Survey No 136, Plot No 2/3/4/5, 1, Mindspace Road, Gachibowli, Hyderabad 500032, Telangana, India.

Background: Mortality due to COVID-19 caused by SARS-CoV-2 infection varies among populations. Functional relevance of genetic variations in Angiotensin-converting enzyme 2 () and Transmembrane serine protease 2 (), two crucial host factors for viral entry, might explain some of this variation.

Methods: In this comparative study in Indian subjects, we recruited 510 COVID-19 patients and retrieved DNA from 520 controls from a repository. Read More

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September 2021

Revealing atomic-scale molecular diffusion of a plant-transcription factor WRKY domain protein along DNA.

Proc Natl Acad Sci U S A 2021 Jun;118(23)

Department of Physics and Astronomy, University of California, Irvine, CA 92697;

Transcription factor (TF) target search on genome is highly essential for gene expression and regulation. High-resolution determination of TF diffusion along DNA remains technically challenging. Here, we constructed a TF model system using the plant WRKY domain protein in complex with DNA from crystallography and demonstrated microsecond diffusion dynamics of WRKY on DNA by employing all-atom molecular-dynamics (MD) simulations. Read More

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Virus-Host Interaction Gets . PART I: Phage P1 Enhanced Development in an DksA-Deficient Cell.

Int J Mol Sci 2021 May 31;22(11). Epub 2021 May 31.

Department of Bacterial Molecular Genetics, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.

Bacteriophage P1 is among the best described bacterial viruses used in molecular biology. Here, we report that deficiency in the host cell DksA protein, an global transcription regulator, improves P1 lytic development. Using genetic and microbiological approaches, we investigated several aspects of P1 biology in an attempt to understand the basis of this phenomenon. Read More

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Chromosome and Molecular Analyses Reveal Significant Karyotype Diversity and Provide New Evidence on the Origin of .

Plants (Basel) 2021 May 11;10(5). Epub 2021 May 11.

Federal Research Center "Fundamentals of Biotechnology", Russian Academy of Sciences, 60 let Oktjabrya Prospect 7, Build. 1, 117312 Moscow, Russia.

Zhuk. is tetraploid grass species (2n = 4x = 28, UUXX) closely related to and growing in Western Asia and a western part of the Fertile Crescent. Genetic diversity of was assessed using C-banding, FISH, nuclear and chloroplast (cp) DNA analyses, and gliadin electrophoresis. Read More

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NFAT5-Mediated Signalling Pathways in Viral Infection and Cardiovascular Dysfunction.

Int J Mol Sci 2021 May 4;22(9). Epub 2021 May 4.

Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC V6T 2B5, Canada.

The nuclear factor of activated T cells 5 (NFAT5) is well known for its sensitivity to cellular osmolarity changes, such as in the kidney medulla. Accumulated evidence indicates that NFAT5 is also a sensitive factor to stress signals caused by non-hypertonic stimuli such as heat shock, biomechanical stretch stress, ischaemia, infection, etc. These osmolality-related and -unrelated stimuli can induce NFAT5 upregulation, activation and nuclear accumulation, leading to its protective role against various detrimental effects. Read More

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