165,585 results match your criteria dna low

"Aptamer-locker" DNA coupling with CRISPR/Cas12a-guided biosensing for high-efficiency melamine analysis.

Biosens Bioelectron 2021 Apr 7;183:113233. Epub 2021 Apr 7.

State Key Laboratory of Biocatalysts and Enzyme Engineering, Hubei University, China; Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, China. Electronic address:

Herein, we report a method that combined "aptamer-locker" DNA with CRISPR/Cas12a-based biosensing for sensitive and rapid melamine analysis. Three strategies were harnessed for designing the DNA sensors that were well characterized by circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC) in the absence and presence of melamine. The detection parameters were optimized to achieve good analytic performance. Read More

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Multiplexing Mutation Rate Assessment: Determining Pathogenicity of Msh2 Variants in S. cerevisiae.

Genetics 2021 Apr 12. Epub 2021 Apr 12.

Genome Sciences Department, University of Washington, Seattle, WA 98195, USA.

Despite the fundamental importance of mutation rate as a driving force in evolution and disease risk, common methods to assay mutation rate are time consuming and tedious. Established methods such as fluctuation tests and mutation accumulation experiments are low-throughput and often require significant optimization to ensure accuracy. We established a new method to determine the mutation rate of many strains simultaneously by tracking mutation events in a chemostat continuous culture device and applying deep sequencing to link mutations to alleles of a DNA-repair gene. Read More

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Prednisone reduction for metastatic castration-resistant prostate cancer with recurrent pulmonary tuberculosis: Case report.

Medicine (Baltimore) 2021 Apr;100(15):e25584

Department of Radiation Oncology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.

Introduction: Prednisone (10 mg/d) is often used in combination with docetaxel or abiraterone in the treatment of advanced prostate cancer. LATITUDE studies have confirmed that the combination of abiraterone and prednisone (5 mg/d) can be used for the treatment of newly diagnosed high-risk metastatic castration-sensitive prostate cancer, and have achieved satisfactory results. However, it has not been reported that abiraterone combined with prednisone (5 mg/d) in the treatment of metastatic castration-resistant prostate cancer (mCRPC). Read More

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Low diversity of mitochondrial DNA in fancy pigeons (Columba livia) revealed by partial D-loop sequencing.

Anim Genet 2021 Apr 13. Epub 2021 Apr 13.

Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice, 32-083, Poland.

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Bimodular thrombin aptamers with two types of non-covalent locks.

Nucleosides Nucleotides Nucleic Acids 2021 Apr 13:1-19. Epub 2021 Apr 13.

Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation.

Aptamers are structured oligonucleotides that specifically bind their targets. Oligonucleotides can be assembled in large nanostructures via intermolecular duplexes or G-quadruplexes. Addition of aptamers can be used to create nanostructures that bind specifically certain targets. Read More

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Progesterone suppresses the progression of colonic carcinoma by increasing the activity of the GADD45α/JNK/c‑Jun signalling pathway.

Oncol Rep 2021 Jun 13;45(6). Epub 2021 Apr 13.

College of Medicine, Southwest Jiaotong University, Chengdu, Sichuan 610031, P.R. China.

Colorectal cancer (CRC) is the third most diagnosed cancer worldwide. Progesterone is associated with a decreased risk of CRC and leads to a favourable prognosis. However, the specific mechanism by which progesterone suppresses malignant progression remains to be elucidated. Read More

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Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues.

Nat Biotechnol 2021 Apr 12. Epub 2021 Apr 12.

Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.

In contrast to single-cell approaches for measuring gene expression and DNA accessibility, single-cell methods for analyzing histone modifications are limited by low sensitivity and throughput. Here, we combine the CUT&Tag technology, developed to measure bulk histone modifications, with droplet-based single-cell library preparation to produce high-quality single-cell data on chromatin modifications. We apply single-cell CUT&Tag (scCUT&Tag) to tens of thousands of cells of the mouse central nervous system and probe histone modifications characteristic of active promoters, enhancers and gene bodies (H3K4me3, H3K27ac and H3K36me3) and inactive regions (H3K27me3). Read More

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Triage of human papillomavirus infected women by methylation analysis in first-void urine.

Sci Rep 2021 Apr 12;11(1):7862. Epub 2021 Apr 12.

Centre for the Evaluation of Vaccination (CEV), Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Campus Drie Eiken, Building S2, Universiteitsplein 1, 2610, Wilrijk, Belgium.

Host cell DNA methylation analysis in urine provides promising triage markers for women diagnosed with a high-risk (HR) human papillomavirus (HPV) infection. In this study, we have investigated a panel of six host cell methylation markers (GHSR, SST, ZIC1, ASCL1, LHX8, ST6GALNAC5) in cervicovaginal secretions collected within the first part of the urine void (FVU) from a referral population. Cytology, histology, and HPV DNA genotyping results on paired FVU and cervical samples were available. Read More

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Hypersonic impact properties of pristine and hybrid single and multi-layer CN and BC nanosheets.

Sci Rep 2021 Apr 12;11(1):7972. Epub 2021 Apr 12.

Mining and Geological Engineering Department, The University of Arizona, Arizona, USA.

Carbon, nitrogen, and boron nanostructures are promising ballistic protection materials due to their low density and excellent mechanical properties. In this study, the ballistic properties of C3N and BC3 nanosheets against hypersonic bullets with Mach numbers greater than 6 were studied. The critical perforation conditions, and thus, the intrinsic impact strength of these 2D materials were determined by simulating ballistic curves of C3N and BC3 monolayers. Read More

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Helicase-like functions in phosphate loop containing beta-alpha polypeptides.

Proc Natl Acad Sci U S A 2021 Apr;118(16)

Department of Biomolecular Sciences, Weizmann Institute of Science, 7610001 Rehovot, Israel;

The P-loop Walker A motif underlies hundreds of essential enzyme families that bind nucleotide triphosphates (NTPs) and mediate phosphoryl transfer (P-loop NTPases), including the earliest DNA/RNA helicases, translocases, and recombinases. What were the primordial precursors of these enzymes? Could these large and complex proteins emerge from simple polypeptides? Previously, we showed that P-loops embedded in simple βα repeat proteins bind NTPs but also, unexpectedly so, ssDNA and RNA. Here, we extend beyond the purely biophysical function of ligand binding to demonstrate rudimentary helicase-like activities. Read More

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Evaluation of real time polymerase chain reaction targeting mpb64 gene for diagnosis of extrapulmonary tuberculosis.

Indian J Tuberc 2021 Apr 12;68(2):242-248. Epub 2020 Sep 12.

Department of Microbiology, JIPMER, Puducherry, India. Electronic address:

Background: Paucibacillary nature of extrapulmonary tuberculosis (EPTB) has paved way for molecular methods increasingly being used for diagnosis. We undertook a study for evaluation of sensitivity and specificity of real-time polymerase chain reaction (RT-PCR) targeting mpb64 gene for diagnosis of EPTB.

Methods: A total of 152 clinical samples from suspected cases of EPTB were included in this study. Read More

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Genetic and Epigenetic Biomarkers For Diagnosis, Prognosis and Treatment Of Metabolic Syndrome.

Curr Pharm Des 2021 Apr 12. Epub 2021 Apr 12.

Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo. Italy.

Background: Metabolic syndrome is a clinical condition that deserves special attention because it puts the individual at high cardiovascular risk, especially heart attack and stroke. Considering the precision medicine, it would be advisable to valuate the individual cardio-metabolic risk by estimating the coesistence of risk factors (abdominal obesity, low level of High- Density Lipoprotein Cholesterol, High Triglycerides, and small dense Low-Density Lipoproteins sub-classes, hypertension, and elevated fasting glycemia), which could engrave on metabolism increasing cardiovascular mortality.

Objective: To identify genetic and epigenetic biomarkers may assist in the possibility of helping follow-up strategies and other measures of prevention, and in metabolic risk. Read More

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Comprehensive Analysis of DNA Adducts Using Data-Independent wSIM/MS Acquisition and wSIM-City.

Anal Chem 2021 Apr 12. Epub 2021 Apr 12.

Masonic Cancer Center, University of Minnesota, Minneapolis 55455, Minnesota, United States.

A novel software has been created to comprehensively characterize covalent modifications of DNA through mass spectral analysis of enzymatically hydrolyzed DNA using the neutral loss of 2'-deoxyribose, a nearly universal MS fragmentation process of protonated 2'-deoxyribonucleosides. These covalent modifications termed DNA adducts form through xenobiotic exposures or by reaction with endogenous electrophiles and can induce mutations during cell division and initiate carcinogenesis. DNA adducts are typically present at trace levels in the human genome, requiring a very sensitive and comprehensive data acquisition and analysis method. Read More

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[Evaluation of spoligotyping-smear sputum as an alternative and culture-independent methodology for Mycobacterium tuberculosis genotyping].

Rev Chilena Infectol 2021 Feb;38(1):61-68

Universidad Nacional Autónoma de Honduras, Honduras.

Background: Tuberculosis (TB) remains as the second cause of death by an infectious disease preceded by the acquired immune deficiency syndrome (AIDS). Currently, laboratory techniques and methodologies of diagnosis and drug susceptibility testing are constantly changing. Therefore, it has been recommended the introduction of rapid assays based on the amplification of nucleic acids test (NAAT's) through a polymerase chain reaction (PCR). Read More

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February 2021