1,723,827 results match your criteria dna

Hemoglobin Yamagata [132(H10)Lys→Asn; (: c.399A>T)]: a mosaic to be put together.

Clin Chem Lab Med 2021 Apr 22. Epub 2021 Apr 22.

Human Genetics Laboratory, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Giannina Gaslini, Genova, Italy.

Objectives: Artifactually altered glycated hemoglobin (HbA) concentrations are frequently linked to hemoglobin (Hb) variants. Their expression and detection require in-depth analysis.

Methods: Cation exchange high performance liquid chromatography (HPLC) (Bio-Rad Variant™ II; Trinity Biotech Premier Hb9210 Resolution), capillary electrophoresis (CE) (Sebia Capillarys 2 Flex Piercing) and mass spectrometry (MS) (Waters) were used for variant detection; Sanger sequencing, multiplex ligation-dependent probe amplification (MLPA) and next generation sequencing (NGS) were used for DNA analysis; HbA was measured with cation exchange HPLC (Bio-Rad Variant™ II; Arkray Adams HA-8180V; Tosoh HLC-723 G7), CE (Sebia Capillarys 2 Flex Piercing), boronate affinity HPLC (Trinity Biotech Hb9210 Premier), immunoassay (Cobas c501 Tina-quant HbA Gen. Read More

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Comparative Cytogenetics in Four Leptodactylus Species (Amphibia, Anura, Leptodactylidae): Evidence of Inner Chromosomal Diversification in Highly Conserved Karyotypes.

Cytogenet Genome Res 2021 Apr 22:1-11. Epub 2021 Apr 22.

Laboratório de Biologia Molecular, Evolução e Microbiologia, Instituto Federal do Pará, Abaetetuba, Brazil.

With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. Read More

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Prospective experimental treatment of colorectal cancer patients based on organoid drug responses.

ESMO Open 2021 Apr 19;6(3):100103. Epub 2021 Apr 19.

Department of Molecular Oncology and Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands; Oncode Institute, Utrecht, The Netherlands; Department of Gastrointestinal Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands. Electronic address:

Background: Organoid technology has recently emerged as a powerful tool to assess drug sensitivity of individual patient tumors in vitro. Organoids may therefore represent a new avenue for precision medicine, as this circumvents many of the complexities associated with DNA- or transcriptional-profiling.

Materials And Methods: The SENSOR trial was a single-arm, single-center, prospective intervention trial to evaluate the feasibility of patient-derived organoids to allocate patients for treatment with off-label or investigational agents. Read More

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Oral cytological changes in young adults related to alcohol consumption.

Arch Oral Biol 2021 Apr 16;126:105127. Epub 2021 Apr 16.

Programa de Pós graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil; Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil; Centro Regional de Estudos, Prevenção e Recuperação de Dependentes Químicos - CENPRE Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil. Electronic address:

Objective: The study aimed to investigate the oral cytological changes in young adults with recent history of alcohol consumption, as well as its relation with the consumption of alcohol.

Design: The sample included 67 young adults, who performed a smear of the oral mucosa and answered a questionnaire about recent and lifetime consumption of alcohol and other drugs. The methods used were sensitive to show the association between alcohol consumption and other drugs with the damage to oral cavity cells. Read More

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Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method.

Ultrason Sonochem 2021 Apr 19;74:105552. Epub 2021 Apr 19.

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. Electronic address:

As a basic technique of molecular cloning, bio-transformation has been successfully used in the fields of biomedicine and food processing. In this study, we established a transformation system of exogenous DNA into E. coli cells mediated by ultrasound. Read More

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Nanoporous hydrogel for direct digital nucleic acid amplification in untreated complex matrices for single bacteria counting.

Biosens Bioelectron 2021 Mar 30;184:113199. Epub 2021 Mar 30.

College of Biosystems Engineering and Food Science, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Fuli Institute of Food Science, Zhejiang University, Hangzhou, China; Ningbo Research Institute, Zhejiang University, Ningbo, 315100, People's Republic of China. Electronic address:

Direct quantification of pathogens in unprocessed complex samples remain challenging due to the severe inhibition of nucleic acid amplification. In this work, we report a nanoporous polyethylene glycol hydrogel with self-cleaning capacity for direct amplification of nucleic acid in complex matrices (human whole blood, animal blood, milky tea, humic acid, and surfactants) without any sample pretreatment or DNA extraction. During isothermal amplification inside the hydrogel, the inhibitors in the assay will be adsorbed and removed by the surrounding nanostructured polymers, and nucleic acid amplification was proceeding successfully, resulting in a series of bright dots for single bacteria counting. Read More

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Activation of NRF2 by APE1/REF1 is redox-dependent in Barrett's related esophageal adenocarcinoma cells.

Redox Biol 2021 Apr 19;43:101970. Epub 2021 Apr 19.

Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL, 33136, USA; Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL-33136, USA; Department of Veterans Affairs, Miami Healthcare System, Miami, FL, 33136, USA. Electronic address:

Background: Chronic gastroesophageal reflux disease (GERD) is a major risk factor for the development of metaplastic Barrett's esophagus (BE) and its progression to esophageal adenocarcinoma (EAC). Uncontrolled accumulation of reactive oxygen species (ROS) in response to acidic bile salts (ABS) in reflux conditions can be lethal to cells. In this study, we investigated the role of APE1/REF1 in regulating nuclear erythroid factor-like 2 (NRF2), the master antioxidant transcription factor, in response to reflux conditions. Read More

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Epigenome-wide DNA methylation signature of benzo[a]pyrene exposure and their mediation roles in benzo[a]pyrene-associated lung cancer development.

J Hazard Mater 2021 Apr 8;416:125839. Epub 2021 Apr 8.

Department of Occupational and Environmental Health, State Key Laboratory of Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Benzo[a]pyrene (B[a]P) is a typical carcinogen associated with increased lung cancer risk, but the underlying mechanisms remain unclear. This study aimed to investigate epigenome-wide DNA methylation associated with B[a]P exposure and their mediation effects on B[a]P-lung cancer association in two lung cancer case-control studies of 462 subjects. Their plasma levels of benzo[a]pyrene diol epoxide-albumin (BPDE-Alb) adducts and genome-wide DNA methylations were separately detected in peripheral blood by using enzyme-linked immunosorbent assay (ELISA) and genome-wide methylation arrays. Read More

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Characterization and use of Tetrahymena thermophila artificial chromosome 2 (TtAC2) constructed by biomimetic of macronuclear rDNA minichromosome.

Microbiol Res 2021 Apr 5;248:126764. Epub 2021 Apr 5.

Department of Biology, Faculty of Sciences, Eskisehir Technical University, Yunusemre Campus, Eskisehir, 26470, Turkey. Electronic address:

Efficient expression vectors for unicellular ciliate eukaryotic Tetrahymena thermophila are still needed in recombinant biology and biotechnology applications. Previously, the construction of the T. thermophila Macronuclear Artificial Chromosome 1 (TtAC1) vector revealed additional needs for structural improvements such as better in vivo stability and maintenance as a recombinant protein expression platform. Read More

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Exploring the causal inference of shear stress associated DNA methylation in carotid plaque on cardiovascular risk.

Atherosclerosis 2021 Apr 6;325:30-37. Epub 2021 Apr 6.

Central Diagnostics Laboratory, Division Laboratories, Pharmacy, and Biomedical Genetics, University Medical Center Utrecht, University of Utrecht, Utrecht, the Netherlands. Electronic address:

Background And Aims: Atherosclerosis is a lipid-driven inflammatory disease presumably initiated by endothelial activation. Low vascular shear stress is known for its ability to activate endothelial cells. Differential DNA methylation (DNAm) is a relatively unexplored player in atherosclerotic disease development and endothelial dysfunction. Read More

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Universal immunohistochemistry for Lynch Syndrome: a systematic review and meta-analysis of 58,580 colorectal carcinomas.

Clin Gastroenterol Hepatol 2021 Apr 19. Epub 2021 Apr 19.

Department of Clinical Genetics, Leiden University Medical Center. Electronic address:

Background And Aims: Lynch Syndrome is a form of hereditary colorectal cancer (CRC), caused by pathogenic germline variants (PV) in DNA mismatch repair genes. Currently, many Western countries perform universal immunohistochemistry testing on CRC to increase the identification of LS patients and their relatives. For a clear understanding of health benefits and costs, data on its outcomes are required: proportions of LS, sporadic MMR-deficient (MMRd) cases, and unexplained MMRd cases. Read More

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DNA methylation profiling discriminates between malignant pleural mesothelioma and neoplastic or reactive histological mimics.

J Mol Diagn 2021 Apr 19. Epub 2021 Apr 19.

Department of Neuropathology, Institute of Pathology, University Hospital of Heidelberg, Im Neuenheimer Feld 224, 69120 Heidelberg, Germany; Clinical Cooperation Unit Neuropathology, German Consortium for Translational Cancer Research (DKTK), German Cancer Research Center (DKFZ), Im Neuenheimer Feld 224, 69120 Heidelberg, Germany.

Diagnosis of malignant pleural mesothelioma (MPM) is a challenging task because of its potential overlap with other neoplasms or even with reactive conditions. Recently, DNA methylation analysis proved to be an effective tool for tumor diagnosis. In the present study, this approach was tested for MPM diagnosis. Read More

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Circulating plasma tumor DNA is superior to plasma tumor RNA detection in Ewing sarcoma patients.

J Mol Diagn 2021 Apr 19. Epub 2021 Apr 19.

Department of Pediatrics, University of Colorado Denver, Aurora, Colorado. Electronic address:

The detection of tumor-specific nucleic acids from blood is being increasingly used as a method of liquid biopsy and minimal residual disease detection. However, achieving high sensitivity and high specificity remains a challenge. Here, we perform a direct comparison of two droplet digital PCR (ddPCR)-based detection methods, circulating plasma tumor RNA (ptRNA) and circulating plasma tumor DNA (ptDNA), in blood samples from newly diagnosed Ewing sarcoma patients. Read More

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Characterisation and validation of an in vitro transactivation assay based on the 22Rv1/MMTV_GR-KO cell line to detect human androgen receptor agonists and antagonists.

Food Chem Toxicol 2021 Apr 19:112206. Epub 2021 Apr 19.

Department of Food Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea. Electronic address:

We describe the characterisation and validation of an androgen receptor (AR) transactivation assay for detection of AR agonists and antagonists using a stably transfected human prostate cancer cell line. This 22Rv1/mouse mammary tumour virus glucocorticoid knock-out cell line based AR transactivation assay was validated by criteria in Organisation for Economic Cooperation and Development Guidance Document 34 to determine if the assay performed equally well to the AR EcoScreen Assay included in Test Guideline for AR Transactivation (OECD TG 458). There was no Glucocorticoid Receptor (GR) crosstalk, and no changes in the AR DNA sequence in cells after the successful knock out of GR. Read More

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Phenotypic and Molecular Characterisations of Carbapenem-resistant Acinetobacter baumannii Isolates Collected within the EURECA Study.

Int J Antimicrob Agents 2021 Apr 19:106345. Epub 2021 Apr 19.

Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.

Multidrug-resistant Acinetobacter baumannii isolates are key pathogens that contribute to the global burden of antimicrobial resistance. This study aimed to investigate the phenotypic and molecular characteristics of carbapenem-resistant A. baumannii (CRAB) isolates from the EURECA clinical trial. Read More

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Rec(F/O/R) proteins of the nitrogen-fixing cyanobacterium Nostoc PCC7120: In silico and expression analysis.

Gene 2021 Apr 19:145663. Epub 2021 Apr 19.

Cyanobacterial Stress Biology and Biotechnology Section, Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, India; Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai -400094, India. Electronic address:

The high radioresistance of Nostoc sp. strain PCC7120 is indicative of a robust DNA repair pathway. In the absence of NHEJ pathway and the canonical RecBCD proteins, the RecF pathway proteins are expected to play an important role in double strand break repair in this organism. Read More

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Coordinated expression of Jumonji and AHCY under OCT transcription factor control to regulate gene methylation in wood frogs during anoxia.

Gene 2021 Apr 19:145671. Epub 2021 Apr 19.

Department of Biology, Carleton University, Ottawa K1S 5B6, Canada. Electronic address:

Wood frogs (Rana sylvatica) can survive extended periods of whole body freezing. Freezing imparts multiple stresses on cells that include anoxia and dehydration, but these can also be experienced as independent stresses. Under anoxia stress, energy metabolism is suppressed, and pro-survival pathways are prioritized to differentially regulate some transcription factors including OCT1 and OCT4. Read More

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A comprehensive review on time-tested anticancer drug doxorubicin.

Life Sci 2021 Apr 19:119527. Epub 2021 Apr 19.

Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, 603 203 Chengalpattu District, Tamil Nadu, India. Electronic address:

Doxorubicin or Adriamycin, is one of the most widely used chemotherapeutic drug for treating a myriad of cancers. It induces cell death through multiple intracellular targets: reactive oxygen species generation, DNA-adduct formation, topoisomerase II inhibition, histone eviction, Ca and iron hemostasis regulation, and ceramide overproduction. Moreover, doxorubicin-treated dying cells undergo cellular modifications that enable neighboring dendritic cell activation and enhanced presentation of tumor antigen. Read More

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Routine laboratory test enabling the detection of dermatophytes and the identification of Trichophyton rubrum by means of in-house duplex real-time PCR.

J Microbiol Methods 2021 Apr 19:106229. Epub 2021 Apr 19.

Bacteriology, Mycology, Antibiotic Resistance and Hospital Hygiene Service, Department of Microbiology, Laboratoire National de Santé, Dudelange, Luxembourg.

Dermatophytes are responsible, in majority, for fungal infections of skin, hair and nails, and Trichophyton rubrum is the most frequently isolated dermatophyte in humans. The time for dermatophyte growth in culture requires a total of two to four weeks. Molecular methods were developed to improve time to diagnosis and initiation of treatment. Read More

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Roles of ATM and ATR in DNA double strand breaks and replication stress.

Prog Biophys Mol Biol 2021 Apr 20. Epub 2021 Apr 20.

Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, London, SW7 2AZ, UK. Electronic address:

The maintenance of genome integrity is critical for the faithful replication of the genome during cell division and for protecting cells from accumulation of DNA damage, which if left unrepaired leads to a loss of genetic information, a breakdown in cell function and ultimately cell death and cancer. ATM and ATR are master kinases that are integral to homologous recombination-mediated repair of double strand breaks and preventing accumulation of dangerous DNA structures and genome instability during replication stress. While the roles of ATM and ATR are heavily intertwined in response to double strand breaks, their roles diverge in the response to replication stress. Read More

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Safety aspects of kraft lignin fractions: Discussions on the in chemico antioxidant activity and the induction of oxidative stress on a cell-based in vitro model.

Int J Biol Macromol 2021 Apr 19. Epub 2021 Apr 19.

Graduate Program in Genetics, Department of Genetics - Federal University of Paraná (UFPR), Curitiba, PR, Brazil. Electronic address:

Lignin is a complex phenolic biopolymer present in plant cell walls and a by-product of the cellulose pulping industry. Lignin has functional properties, such as antioxidant activity, that make it a potential natural active ingredient for health-care products. However, not all safety aspects of lignin fractions have been adequately investigated. Read More

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Discovery of an imidazonaphthyridine and a riminophenazine as potent anti-Zika virus agents through a replicon-based high-throughput screening.

Virus Res 2021 Apr 19:198388. Epub 2021 Apr 19.

Physics Institute of Sao Carlos, University of Sao Paulo, Av. Joao Dagnone, 1100, Jardim Santa Angelina, São Carlos, 13563-120, Brazil.

The 2015/16 Zika virus (ZIKV) epidemic led to almost 1 million confirmed cases in 84 countries and was associated to the development of congenital microcephaly and Guillain-Barré syndrome. More recently, a ZIKV African lineage was identified in Brazil raising concerns about a future outbreak. The long-term consequences of viral infection emphasizes the need for the development of effective anti-ZIKV drugs. Read More

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Age-related activity of Poly (ADP-ribose) polymerase (PARP) in men with localized prostate cancer.

Mech Ageing Dev 2021 Apr 19:111494. Epub 2021 Apr 19.

Department of Obstetrics and Gynecology, Ulm University, Prittwitzstrasse 43, 89075, Ulm, Germany. Electronic address:

Mutations in DNA repair genes have been connected with familial prostate cancer and sensitivity to targeted drugs like PARP-inhibitors. Clinical use of this information is limited by the small fraction of prostate cancer risk gene carriers, variants of unknown pathogenicity and the focus on monogenic disease mechanisms. Functional assays capturing mono- and polygenic defects were shown to detect breast and ovarian cancer risk in blood-derived cells. Read More

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Oxidative Stress in Vascular Calcification.

Clin Chim Acta 2021 Apr 19. Epub 2021 Apr 19.

Institute of Cardiovascular Disease, Key Lab for Arteriosclerology of Hunan province, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, People's Republic of China. Electronic address:

Vascular calcification (VC), which is closely associated with significant mortality in cardiovascular disease, chronic kidney disease (CKD), and/or diabetes mellitus, is characterized by abnormal deposits of hydroxyapatite minerals in the arterial wall. The impact of oxidative stress (OS) on the onset and progression of VC has not been well described. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, xanthine oxidases, myeloperoxidase (MPO), nitric oxide synthases (NOSs), superoxide dismutase (SOD) and paraoxonases (PONs) are relevant factors that influence the production of reactive oxygen species (ROS). Read More

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Berberinesuppresses bladdercancercell proliferation by inhibiting JAK1-STAT3 signaling via upregulation of miR-17-5p.

Biochem Pharmacol 2021 Apr 19:114575. Epub 2021 Apr 19.

Key Laboratory of Urinary Precision Diagnosis and Treatment, Department of Urology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China. Electronic address:

Hyperactivation of signal transducer and activator of transcription 3 (STAT3) is strongly associated with cancer initiation, progression, metastasis, chemoresistance, and immune evasion; thus, STAT3 has beenintenselystudiedasatherapeutictargetfor cancer treatment. Berberine (BBR), an active component extracted from Coptis chinensis, has shown anti-tumor effects in multiple tumors. However, its underlying mechanisms have not yet been fully elucidated. Read More

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Concerted evolution reveals co-adapted amino acid substitutions in NaK-ATPase of frogs that prey on toxic toads.

Curr Biol 2021 Apr 20. Epub 2021 Apr 20.

Department of Biological Sciences, Columbia University, New York, NY, USA. Electronic address:

Although gene duplication is an important source of evolutionary innovation, the functional divergence of duplicates can be opposed by ongoing gene conversion between them. Here, we report on the evolution of a tandem duplication of Na,K-ATPase subunit α1 (ATP1A1) shared by frogs in the genus Leptodactylus, a group of species that feeds on toxic toads. One ATP1A1 paralog evolved resistance to toad toxins although the other retained ancestral susceptibility. Read More

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Sediment archives reveal irreversible shifts in plankton communities after World War II and agricultural pollution.

Curr Biol 2021 Apr 17. Epub 2021 Apr 17.

CNRS, UMR 6538 Laboratoire Géosciences Océan (LGO), Université de Brest (UBO), 29280 Plouzané, France.

To evaluate the stability and resilience of coastal ecosystem communities to perturbations that occurred during the Anthropocene, pre-industrial biodiversity baselines inferred from paleoarchives are needed. The study of ancient DNA (aDNA) from sediments (sedaDNA) has provided valuable information about past dynamics of microbial species and communities in relation to ecosystem variations. Shifts in planktonic protist communities might significantly affect marine ecosystems through cascading effects, and therefore the analysis of this compartment is essential for the assessment of ecosystem variations. Read More

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sp. nov., isolated from mangrove sediments.

Int J Syst Evol Microbiol 2021 Apr;71(4)

Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, PR China.

A novel Gram-staining-negative, catalase- and oxidase-positive, facultatively anaerobic and rod-shaped motile bacterial strain, designated as ZWAL4003, was isolated from mangrove sediments of the Zini Mangrove Forest, Zhangzhou City, PR China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that ZWAL4003 was grouped into a separated branch with MSSRF60 (97.38% nucleotide sequence identity) and NBRC 103148 (97. Read More

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