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Posterior spinal fixation using penetrating endplate screws in patients with diffuse idiopathic skeletal hyperostosis-related thoracolumbar fractures.

J Neurosurg Spine 2021 Apr 9:1-6. Epub 2021 Apr 9.

1Orthopedic Surgery, Sanmu Medical Center, Chiba, Japan; and.

Objective: Diffuse idiopathic skeletal hyperostosis (DISH)-related vertebral fractures essentially require operative treatment due to severe fracture site instability and high potential risk of posttraumatic neurological deficit. However, the optimal surgical procedure remains unclear. The purpose of this study was to assess the efficacy of posterior spinal fixation with penetrating endplate screws (PESs) for DISH-related thoracolumbar fractures. Read More

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Asian carp: A threat to American lakes, a feast on Chinese tables.

Compr Rev Food Sci Food Saf 2021 Apr 9. Epub 2021 Apr 9.

Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

Asian carp, which are widely distributed in Asia and Europe, are nutritious and popular with consumers. In China, Asian carp is a tasty dish and has been consumed for thousands of years. However, they are considered aggressive invasive species that threaten rivers, lakes, and indigenous species in the United States. Read More

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First Report of Causing Garlic clove Rot in Russian Federation.

Plant Dis 2021 Apr 9. Epub 2021 Apr 9.

FSI Federal Research Centre Fundamentals of Biotechnology of the Russian Academy of Sciences, 442108, Laboratory of plant system biology , Moscow, Moscow, Russian Federation;

Garlic () is a widely consumed bulbous crop both worldwide and in Russia. About 200,000 tons of garlic is produced in Russia annually (https://rosstat.gov. Read More

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Performance of a HER2 testing algorithm specific for p53-abnormal endometrial cancer.

Histopathology 2021 Apr 9. Epub 2021 Apr 9.

Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands.

Aims: HER2 amplification in endometrial cancer (EC) is almost completely confined to the p53-abnormal (p53abn) molecular subtype and independent of histologic subtype. HER2 testing should therefore be molecular subtype-directed. However, the most optimal approach for HER2 testing in EC has not been fully established. Read More

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Satellite-Dish-Shaped Pentagon- and Heptagon-Embedded Nanographene Build on a Central Pyrrolo[3,2-b]pyrrole Core.

Angew Chem Int Ed Engl 2021 Apr 8. Epub 2021 Apr 8.

Institute of Organic Chemistry, Polish Academy of Sciences, Institute of Organic Chemistry, Kasprzaka 44/52, 01-224, Warsaw, POLAND.

A bowl-shaped nitrogen-doped nanographene composed of a pyrrolo[3,2- b ]pyrrole core substituted with six arene rings circularly bonded with one another, has been prepared via a concise synthetic strategy encompassing the multicomponent tetraarylpyrrolopyrrole (TAPP) synthesis, the Scholl reaction and intramolecular direct arylation. This synthesis represents the first case of programmed sequential intramolecular direct arylation reactions utilizing the different reactivity of C-Br and C-Cl bonds. The target non-alternant, nitrogen-embedded polycyclic aromatic hydrocarbon (PAH) contains two central pentagons confined between two adjacent heptagons - the inverse Stone-Thrower-Wales topology. Read More

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Usefulness of dynamic stabilisation with mobile percutaneous pedicle screw for thoracic vertebral fractures in diffuse idiopathic skeletal hyperostosis.

BMJ Case Rep 2021 Apr 7;14(4). Epub 2021 Apr 7.

Department of Orthopaedic Surgery, Chiba University, Chiba, Japan.

We report a case of vertebral fracture with diffuse idiopathic skeletal hyperostosis (DISH) who underwent posterior dynamic stabilisation using mobile percutaneous pedicle screws (PPS) with 1 above-1 below and obtained good bone fusion. A 76-year-old man experienced severe low back pain after he fell backward 1 m off a stepladder during work. A 12th thoracic vertebral fracture with DISH was observed. Read More

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Craniovertebral junction instability in Larsen syndrome: An institutional series and review of literature.

J Craniovertebr Junction Spine 2020 Oct-Dec;11(4):276-286. Epub 2020 Nov 26.

Department of Neurosurgery, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.

Objective: Larsen syndrome (LS) is characterized by osteo-chondrodysplasia, multiple joint dislocations, and craniofacial abnormalities. Symptomatic myelopathy is attributed to C1-C2 instability and sub-axial cervical kyphosis. In this article, we have analyzed the surgical outcome after posterior fixation in LS with craniovertebral junction instability. Read More

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November 2020

Indoor water end-use pattern and its prospective determinants in the twin cities of Gujarat, India: Enabling targeted urban water management strategies.

J Environ Manage 2021 Mar 30;288:112403. Epub 2021 Mar 30.

Graduate School of Social Science, Hitotsubashi University, 2-1 Naka, Kunitachi, Tokyo, 186-8601, Japan.

Water end-use studies disaggregate the quantity and frequency of water uses for various household purposes. Water end-use studies are available but none for India, which is gradually approaching a water-scarce condition from being a water-stressed country at present. This implies a need for incorporating water end-use understanding for augmenting urban recycling plans and strategies. Read More

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Isolation Chip Increases Culturable Bacterial Diversity and Reduces Cultivation Bias.

Curr Microbiol 2021 Apr 5. Epub 2021 Apr 5.

College of Engineering, Peking University, Beijing, 100871, China.

Conventional cultivation methods, including petri dish plating, are selective and biased to enrich specific microorganisms, such as big population and fast-growing bacteria. In this study, we evaluated the ability of isolation chip (ichip) to reduce cultivation bias. We used the ichip and petri dish plating methods to cultivate bacteria from soil contaminated with (contaminated soil) or without (natural soil) crude oil. Read More

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Regular consumption of "Nkui", a Cameroonian traditional dish, may protect against cardiovascular and bone disorders in an estrogen deficiency condition.

J Complement Integr Med 2021 Apr 5. Epub 2021 Apr 5.

Department of Chemical Sciences, Faculty of Science, University of Johannesburg, Johannesburg, South Africa.

Objectives: There is a growing body of evidence indicating the potential of culinary herbs and spices to decrease the incidence of several chronic diseases or conditions. Because of this, the WHO recommends their regular consumption. In the Cameroonian culinary practices, "Nkui" is a famous dish made from a mixture of 10 spices. Read More

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Functional changes of cocultured hepatocyte sheets subjected to continuous liver regeneration stimulation in cDNA-uPA/SCID mouse: Differences in transplantation sites.

Regen Ther 2021 Dec 18;18:7-11. Epub 2021 Mar 18.

Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.

Aim: The formation of a secondary liver is expected in ectopic transplants in liver therapy. It is reported that the transplantation of hepatocyte sheets constitutes one of the techniques used to form a secondary liver. Accordingly, we established a subcutaneous transplant for hepatocyte/fibroblast sheets in previous studies. Read More

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December 2021

Is Harbor Porpoise () Exhaled Breath Sampling Suitable for Hormonal Assessments?

Animals (Basel) 2021 Mar 22;11(3). Epub 2021 Mar 22.

Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, Werftstrasse 6, 25761 Buesum, Germany.

Over the last decades, exhaled breath sampling has been established for laboratory analysis in various cetacean species. Due to their small size, the usability of respiratory vapor for hormone assessments was questionable in harbor porpoises (). This pilot study compared three different blow collection devices for their suitability in the field and during laboratory processing: a sterile petri dish covered by a Nitex membrane, as well as sterile 50 mL centrifuge tubes with or without manganese(II) chloride as a stabilizer. Read More

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Effect of Dietary Supplementation of Immunobiotic N14 Fermented Rakkyo () Pickled Juice on the Immunocompetence and Production Performance of Pigs.

Animals (Basel) 2021 Mar 9;11(3). Epub 2021 Mar 9.

Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.

Rakkyo (), is a Japanese leek that is primarily used to make a popular sweet or sour pickled dish. Lactic acid bacteria are often involved in the preparation steps of fermented pickles, which helps in the effective preservation of the natural bioactive compounds of fruits and vegetable, and thereby exert several health benefits including immunomodulation and growth performance. This work aimed to evaluate the in vivo effects of adding N14 fermented rakkyo pickled juice as feed supplement on the immunocompetence and production performance of pigs. Read More

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An Improved Transwell Design for Microelectrode Ion-Flux Measurements.

Micromachines (Basel) 2021 Mar 6;12(3). Epub 2021 Mar 6.

Institute of Microbiology of the Czech Academy of Sciences, Zamek 136, 37333 Nove Hrady, Czech Republic.

The microelectrode ion flux estimation (MIFE) is a powerful, non-invasive electrophysiological method for cellular membrane transport studies. Usually, the MIFE measurements are performed in a tissue culture dish or directly with tissues (roots, parts of the plants, and cell tissues). Here, we present a transwell system that allows for MIFE measurements on a cell monolayer. Read More

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Characteristics of MSCs in Synovial Fluid and Mode of Action of Intra-Articular Injections of Synovial MSCs in Knee Osteoarthritis.

Int J Mol Sci 2021 Mar 11;22(6). Epub 2021 Mar 11.

Center for Stem Cells and Regenerative Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

We have been studying mesenchymal stem cells (MSCs) in synovial fluid and the intra-articular injection of synovial MSCs in osteoarthritis (OA) knees. Here, mainly based on our own findings, we overview the characteristics of endogenous MSCs in the synovial fluid of OA knees and their mode of action when injected exogenously into OA knees. Many MSCs similar to synovial MSCs were detected in the synovial fluid of human OA knees, and their number correlated with the radiological OA grade. Read More

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Modelling Parkinson's Disease: iPSCs towards Better Understanding of Human Pathology.

Brain Sci 2021 Mar 14;11(3). Epub 2021 Mar 14.

Physiology and CÚRAM SFI Centre for Research in Medical Devices, School of Medicine, National University of Ireland (NUI), H91 TK33 Galway, Ireland.

Parkinson's Disease (PD) is a chronic neurodegenerative disorder characterized by motor and non-motor symptoms, among which are bradykinesia, rigidity, tremor as well as mental symptoms such as dementia. The underlying cause of Parkinson disease is degeneration of dopaminergic neurons. It has been challenging to develop an efficient animal model to accurately represent the complex phenotypes found with PD. Read More

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Resilience of network activity in preconditioned neurons exposed to 'stroke-in-a-dish' insults.

Neurochem Int 2021 Mar 30:105035. Epub 2021 Mar 30.

Human Health Therapeutics Research Center, National Research Council of Canada, Ottawa, ON, Canada, K1A 0R6; Brain and Mind Research Institute, University of Ottawa, ON, Canada, K1N 6N5.

Exposing cultured cortical neurons to stimulatory agents - the K channel blocker 4-aminopyridine (4-ap), and the GABA receptor antagonist bicuculline (bic) - for 48 h induces down-regulated synaptic scaling, and preconditions neurons to withstand subsequent otherwise lethal 'stroke-in-a-dish' insults; however, the degree to which usual neuronal function remains is unknown. As a result, multi-electrode array and patch-clamp electrophysiological techniques were employed to characterize hallmarks of spontaneous synaptic activity during over a 12-day preconditioning/insult experiment. Spiking frequency increased 8-fold immediately upon 4-ap/bic treatment but declined within the 48 h treatment window to sub-baseline levels that persisted long after washout. Read More

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The relationship between fruit and vegetable intake with functional dyspepsia in adults.

Neurogastroenterol Motil 2021 Apr 2:e14129. Epub 2021 Apr 2.

Isfahan Gastroenterology and Hepatology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Introduction: The association of fruit and vegetables with functional dyspepsia (FD) has been less studied, especially in Middle Eastern populations. This study investigated the relationship between fruit and vegetable consumption with FD and its related symptoms among a large group of Iranian adults.

Methods And Materials: This cross-sectional research was conducted on 3362 middle-age participants. Read More

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Malignant ulcer: a great mimicker of gastric plasmablastic lymphoma.

Clin J Gastroenterol 2021 Apr 1. Epub 2021 Apr 1.

Department of Pathobiology and Medical Diagnostic, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia.

Plasmablastic lymphoma (PBL) is an uncommon human immunodeficiency virus (HIV)-associated lymphoma, with a predilection to develop in the oral cavity. It usually has a plasmablastic morphology with weak or no expression of B cell-associated markers. Among non-HIV patients, it tends to occur in the gastrointestinal (GI) tract, lymph nodes, and skin. Read More

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Cadherins in early neural development.

Cell Mol Life Sci 2021 Apr 1. Epub 2021 Apr 1.

Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Little France Drive, Edinburgh, EH16 4UU, UK.

During early neural development, changes in signalling inform the expression of transcription factors that in turn instruct changes in cell identity. At the same time, switches in adhesion molecule expression result in cellular rearrangements that define the morphology of the emerging neural tube. It is becoming increasingly clear that these two processes influence each other; adhesion molecules do not simply operate downstream of or in parallel with changes in cell identity but rather actively feed into cell fate decisions. Read More

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Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders.

Bio Protoc 2021 Mar 5;11(5):e3939. Epub 2021 Mar 5.

Department of General Pediatrics, Neonatology, and Pediatric Cardiology, Heinrich Heine University, Duesseldorf, Germany.

The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery. Read More

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Dystrophic calcification and heterotopic ossification in fibrocartilaginous tissues of the spine in diffuse idiopathic skeletal hyperostosis (DISH).

Bone Res 2020 Apr 2;8(1):16. Epub 2020 Apr 2.

Bone and Joint Institute, The University of Western Ontario, London, ON, N6G 2V4, Canada.

Diffuse idiopathic skeletal hyperostosis (DISH) is a prevalent noninflammatory spondyloarthropathy characterized by ectopic mineral formation along the anterolateral aspect of the vertebral column, yet little is known about its underlying pathogenesis. Our objective was to evaluate the histopathological features and composition of ectopic mineral within spinal tissues affected by DISH in humans. Thoracic spine segments from six embalmed cadaveric donors (one female and five males; median age 82 years) meeting the radiographic diagnostic criteria for DISH were evaluated using radiological, histological, and physical analyses. Read More

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First Report of Ug99 Race TTKTT of Wheat Stem Rust (Puccinia graminis f. sp. tritici) in Iraq.

Plant Dis 2021 Mar 29. Epub 2021 Mar 29.

Aegean Agricultural Research Institute Directorate, 518332, Turkey-ICARDA Regional Cereal Rust Research Center (RCRRC), P.O. Box 35661, Menemen, Izmir, Turkey;

A wheat rust survey was conducted in Iraq in 2019 and collected 27 stem rust (caused by Puccinia graminis Pers.:Pers. f. Read More

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Heart organoids and tissue models for modeling development and disease.

Semin Cell Dev Biol 2021 Mar 26. Epub 2021 Mar 26.

Division of Cardiology, Department of Medicine, Johns Hopkins University, Baltimore, MD, United States; Heart and Vascular Institute, Cellular and Molecular Medicine, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States; Department of Biomedical Engineering, Department of Cell Biology, Johns Hopkins University, Baltimore, MD, United States. Electronic address:

Organoids, or miniaturized organs formed in vitro, hold potential to revolutionize how researchers approach and answer fundamental biological and pathological questions. In the context of cardiac biology, development of a bona fide cardiac organoid enables study of heart development, function, and pathogenesis in a dish, providing insight into the nature of congenital heart disease and offering the opportunity for high-throughput probing of adult heart disease and drug discovery. Recently, multiple groups have reported novel methods for generating in vitro models of the heart; however, there are substantial conceptual and methodological differences. Read More

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What Do Mom and Dad Think? Examining Parent Preferences for the Use of Behavioral Economic Strategies on Children's Menus in Restaurants.

J Acad Nutr Diet 2021 Mar 24. Epub 2021 Mar 24.

Background: A majority of children's restaurant meals are nutritionally deficient; use of behavioral economics may improve healthful menu selections. Parents play a role in children's restaurant meal selection, thus understanding parent preferences for potential behavioral economic strategies is warranted.

Objective: To examine parent-derived and -stated preferences for selected attributes of children's restaurant menus, which included behavioral economic strategies (eg, optimal defaults and vice-virtue bundles). Read More

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New Avenues for Treatment and Prevention of Drug-Induced Steatosis and Steatohepatitis: Much More Than Antioxidants.

Adv Ther 2021 Mar 24. Epub 2021 Mar 24.

Department of Clinical Medicine and Surgery, "Federico II" University Medical School of Naples, Naples, Italy.

Drug-induced lipid accumulation in the liver may induce two clinically relevant conditions, drug-induced steatosis (DIS) and drug-induced steatohepatitis (DISH). The list of drugs that may cause DIS or DISH is long and heterogeneous and includes therapeutically relevant molecules that cannot be easily replaced by less hepatotoxic medicines, therefore making specific strategies necessary for DIS/DISH prevention or treatment. For years, the only available tools to achieve these goals have been antioxidant drugs and free radical scavengers, which counteract drug-induced mitochondrial dysfunction but, unfortunately, have only limited efficacy. Read More

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Observation of Cells on a Simulated Titanium Surface with Transparency.

J Dent Res 2021 Mar 23:220345211000272. Epub 2021 Mar 23.

National Institute of Advanced Industrial Science and Technology, GaN Advanced Device Open Innovation Laboratory, Nagoya, Aichi, Japan.

The main driving force of osseointegration on titanium implants is believed to be the calcification caused by cellular activity. However, owing to the opacity of bulk titanium, live cells on titanium surfaces cannot be observed using an inverted microscope. To overcome this limitation, this study proposes a transparent titanium thin layer as a simulated titanium surface that allows live-cell observation from below. Read More

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First Report of Crown and Root Rot Caused by Pythium myriotylum on Hemp (Cannabis sativa) in Arizona.

Authors:
Jiahuai Hu

Plant Dis 2021 Mar 23. Epub 2021 Mar 23.

University of Arizona, School of Plant Sciences, 1140 E. South Campus Dr., Forbes Building, Room 303, Tucson, Arizona, United States, 85721;

During August and September 2020, symptoms of leaf chlorosis, stunting, and wilting were observed on indoor hemp plants (Cannabis sativa L. cv. 'Wedding Cake') in a commercial indoor facility located in Coolidge, Arizona. Read More

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Performance of SwiftScan planar and SPECT technology using low-energy high-resolution and sensitivity collimator compared with Siemens SPECT system.

Nucl Med Commun 2021 Mar 18. Epub 2021 Mar 18.

Department of Quantum Medical Technology, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University Department of Radiological Technology, Kanazawa University Hospital Department of Functional Imaging and Artificial Intelligence, Kanazawa University, Kanazawa, Ishikawa, Japan.

Purpose: A new low-energy high-resolution-sensitivity (LEHRS) collimator was developed by General Electric (GE) Healthcare. SwiftScan planar and single photon emission computed tomography (SPECT) systems using LEHRS collimator were developed to achieve the low-dose and/or short-time acquisition. We demonstrated the performance of SwiftScan planar and SPECT system with LEHRS collimator using phantoms. Read More

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