4,781 results match your criteria digital pcr


Copy number variation of the SRY gene showed an association with disorders of sex development in Yorkshire Terrier dogs.

Anim Genet 2021 Oct 19. Epub 2021 Oct 19.

Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, Poznan, 60-637, Poland.

The molecular background of disorders of sex development (DSD) in dogs is poorly understood. Several copies of the SRY genes have been reported in the dog genome. We used droplet digital PCR with the aim of determining variability in SRY copy number and its association with DSD in dogs. Read More

View Article and Full-Text PDF
October 2021

Presence of defective viral genes in H1N1 live attenuated influenza vaccine strains is not associated with reduced human cell fitness or vaccine effectiveness.

Vaccine 2021 Oct 15. Epub 2021 Oct 15.

Flu-BPD, Biopharmaceuticals R&D, AstraZeneca, Liverpool, UK.

In the 2013-2014 and 2015-2016 seasons, quadrivalent live attenuated influenza vaccine (LAIV) showed reduced pandemic 2009 H1N1 (A/H1N1pdm09) vaccine effectiveness (VE) in the U.S. Impaired fitness of A/H1N1pdm09 LAIV strains in primary human nasal epithelial cells (hNEC) was subsequently shown to be associated with reduced VE. Read More

View Article and Full-Text PDF
October 2021

Development and Efficacy of Droplet Digital PCR for Detection of Strongyloides stercoralis in Stool.

Am J Trop Med Hyg 2021 Oct 18. Epub 2021 Oct 18.

Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

Human strongyloidiasis is one of the neglected tropical diseases caused by infection with soil-transmitted helminth Strongyloides stercoralis. Conventional stool examination, a method commonly used for diagnosis of S. stercoralis, has low sensitivity, especially in the case of light infections. Read More

View Article and Full-Text PDF
October 2021

Oral oligofructose challenge reduces expression of glucose transport-1 and 5'-adenosine monophosphate-activated protein kinase in lamellar wall of Holstein heifer claw.

Res Vet Sci 2021 Oct 11;141:42-47. Epub 2021 Oct 11.

Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin 150030, PR China. Electronic address:

The laminar tissue of bovine laminitis may undergo energy failure. The expression of glucose transport protein-1 (GLUT-1) and 5'-adenosine monophosphate-activated protein kinase (AMPK) affects the energy metabolism of digital laminar tissue. This study aimed to determine the expression of glucose uptake and AMPK in laminar wall corium of Holstein heifer claw by oral administration of oligofructose. Read More

View Article and Full-Text PDF
October 2021

Defense by duplication: The relation between phenotypic glyphosate resistance and EPSPS gene copy number variation in Amaranthus palmeri.

Mol Ecol 2021 Oct 18. Epub 2021 Oct 18.

Department of Integrative Biology, University of Guelph, Guelph, ON, Canada.

Gene copy number variation (CNV) has been increasingly associated with organismal responses to environmental stress, but we know little about the quantitative relation between CNV and phenotypic variation. In this study we quantify the relation between variation in EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) copy number using digital drop PCR and variation in phenotypic glyphosate resistance in 22 populations of Amaranthus palmeri (Palmer Amaranth), a range-expanding agricultural weed. Overall, we detected a significant positive relation between population mean copy number and resistance. Read More

View Article and Full-Text PDF
October 2021

Replication and transmission of an influenza A(H3N2) virus harboring the polymerase acidic I38T substitution, in guinea pigs.

J Gen Virol 2021 Oct;102(10)

Research Center in Infectious Diseases of the "CHU de Québec" and Laval University, Québec City, Québec, Canada.

The polymerase acidic (PA) I38T substitution is a dominant marker of resistance to baloxavir. We evaluated the impact of I38T on the fitness of a contemporary influenza A(H3N2) virus. Influenza A/Switzerland/9715293/2013 (H3N2) wild-type (WT) virus and its I38T mutant were rescued by reverse genetics. Read More

View Article and Full-Text PDF
October 2021

Diagnostic performances of common nucleic acid tests for SARS-CoV-2 in hospitals and clinics: a systematic review and meta-analysis.

Lancet Microbe 2021 Oct 13. Epub 2021 Oct 13.

Department of Chemical Pathology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, China.

Background: An optimised standard experimental setup across different hospitals is urgently needed to ensure consistency in nucleic acid test results for SARS-CoV-2 detection. A standard comparison across different nucleic acid tests and their optimal experimental setups is not present. We assessed the performance of three common nucleic acid tests, namely digital PCR (dPCR), quantitative PCR (qPCR), and loop-mediated isothermal amplification (LAMP), to detect SARS-CoV-2 in clinical settings. Read More

View Article and Full-Text PDF
October 2021

Identification of Loci Enabling Stable and High-Level Heterologous Gene Expression.

Front Bioeng Biotechnol 2021 1;9:734902. Epub 2021 Oct 1.

Toulouse Biotechnology Institute (TBI), Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.

Efficient and reliable genome engineering technologies have yet to be developed for diatoms. The delivery of DNA in diatoms results in the random integration of multiple copies, quite often leading to heterogeneous gene activity, as well as host instability. Transgenic diatoms are generally selected on the basis of transgene expression or high enzyme activity, without consideration of the copy number or the integration locus. Read More

View Article and Full-Text PDF
October 2021

Asymptomatic and Mild SARS-CoV-2 Infections Elicit Lower Immune Activation and Higher Specific Neutralizing Antibodies in Children Than in Adults.

Front Immunol 2021 30;12:741796. Epub 2021 Sep 30.

Oncology and Immunology Section, Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy.

Background: The immune response plays a pivotal role in dictating the clinical outcome in severe acute respiratory syndrome coronavirus 2 (SARSCoV2)-infected adults, but it is still poorly investigated in the pediatric population.

Methods: Of 209 enrolled subjects, 155 patients were confirmed by PCR and/or serology as having coronavirus disease 2019 (COVID-19). Blood samples were obtained at a median of 2. Read More

View Article and Full-Text PDF
September 2021

A digitalized isothermal nucleic acid testing platform based on a pump-free open droplet array microfluidic chip.

Analyst 2021 Oct 18. Epub 2021 Oct 18.

Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, P.R. China.

Digital PCR has shown great potential for quantitative nucleic acid testing (NAT), but most existing platforms are dependent on large auxiliary equipment (, vacuum pump, amplification instrument, fluorescence microscope) to achieve target dispersion, amplification, signal capture and result analysis. Such complex, expensive and bulky NAT platforms have limited their applications in resource-limited areas, especially for point-of-care testing (POCT). In this work, we designed a digital isothermal NAT platform based on a pump-free open droplet array microfluidic chip. Read More

View Article and Full-Text PDF
October 2021

Droplet digital polymerase chain reaction for the assessment of disease burden in hairy cell leukemia.

Hematol Oncol 2021 Oct 15. Epub 2021 Oct 15.

IRCCS Azienda Ospedaliero-Universitaria di Bologna, Istituto di Ematologia "Seràgnoli", Bologna, Italy.

BRAF mutation is the pathogenic driver of hairy cell leukemia (HCL) found in the vast majority of cases both at onset and during recurrences. The identification of the mutated allele in blood and marrow correlates with the presence of neoplastic cells and can be considered a marker of active disease. Likewise, the absence of the mutation after treatment may indicate a state of deep response. Read More

View Article and Full-Text PDF
October 2021

Analysis of mammograms using artificial intelligence to predict response to neoadjuvant chemotherapy in breast cancer patients: proof of concept.

Eur Radiol 2021 Oct 15. Epub 2021 Oct 15.

Medical Radiation Physics, Department of Translational Medicine, Lund University, Skane University Hospital, Malmö, Sweden.

Objectives: In this proof of concept study, a deep learning-based method for automatic analysis of digital mammograms (DM) as a tool to aid in assessment of neoadjuvant chemotherapy (NACT) treatment response in breast cancer (BC) was examined.

Methods: Baseline DM from 453 patients receiving NACT between 2005 and 2019 were included in the study cohort. A deep learning system, using the aforementioned baseline DM, was developed to predict pathological complete response (pCR) in the surgical specimen after completion of NACT. Read More

View Article and Full-Text PDF
October 2021

Correlation of transrenal DNA with non-small-cell lung cancer in noninvasive disease monitoring.

Biomark Med 2021 Oct 15. Epub 2021 Oct 15.

Department of Internal Medicine, Wuhan No. 6 Hospital, Affiliated Hospital to Jianghan University, Wuhan, People's Republic of China.

The study aims to use noninvasive transrenal DNA in advanced non-small-cell lung cancer (NSCLC) patients for treatment monitoring and prognosis. Urine specimens were collected longitudinally for 103 late-stage NSCLC patients. Detection of targetable mutations in transrenal DNA was achieved by digital droplet PCR. Read More

View Article and Full-Text PDF
October 2021

Evaluation of EpCAM-specific exosomal lncRNAs as potential diagnostic biomarkers for lung cancer using droplet digital PCR.

J Mol Med (Berl) 2021 Oct 14. Epub 2021 Oct 14.

Department of Thoracic Surgery, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, 315020, Zhejiang, China.

Accumulating evidence demonstrated that long non-coding RNAs (lncRNAs) derived from exosomes had the potential to be diagnostic markers for lung cancer. However, the diagnostic value of lncRNAs from epithelial cell adhesion molecule (EpCAM)-positive exosomes remains unclear. In the study, serum EpCAM-positive exosomes were isolated with magnetic beads, and their role in lung cancer was investigated in vitro and in vivo. Read More

View Article and Full-Text PDF
October 2021

Bockenheimer Disease is Associated With a TEK Variant.

Cold Spring Harb Mol Case Stud 2021 Oct 14. Epub 2021 Oct 14.

Boston Children's Hospital, Harvard Medical School.

Bockenheimer disease is a venous malformation involving all tissues of an extremity. Patients have significant morbidity and treatment is palliative. The purpose of this study was to identify the cause of Bockenheimer disease to develop pharmacotherapy for the condition. Read More

View Article and Full-Text PDF
October 2021

Identification of a recurrent mosaic variant in brain tissue from an individual with nevus sebaceous syndrome.

Cold Spring Harb Mol Case Stud 2021 Oct 14. Epub 2021 Oct 14.

Epilepsy Research Centre, Department of Medicine (Austin Hospital), University of Melbourne, Heidelberg, Victoria, Australia; Murdoch Children Research Institute, Parkville, Victoria, Australia

Nevus sebaceous syndrome (NSS) is a rare multisystem neurocutaneous syndrome, characterised by a congenital nevus, and may include brain malformations such as hemimegalencephaly or focal cortical dysplasia, ocular and skeletal features. It has been associated with several eponyms including Schimmelpenning and Jadassohn. The isolated skin lesion, nevus sebaceous, is associated with post-zygotic variants in or in all patients studied. Read More

View Article and Full-Text PDF
October 2021

ANGPT1 and ANGPT2 polymorphisms in systemic sclerosis: ANGPT2 rs2442598 and rs3739390 are associated with disease susceptibility and diffuse subtype. Preliminary observations.

Pol Arch Intern Med 2021 Oct 13. Epub 2021 Oct 13.

Introduction: Angiopoietin-1 (Ang1) and -2 (Ang2) were found to be associated with systemic sclerosis (SSc).

Objectives: We explored whether the single nucleotide polymorphisms of Ang1 (ANGPT1) and Ang2 (ANGPT2) genes can predict the SSc susceptibility in Polish Caucasians.

Patients And Methods: Genotyping by RT-PCR and Sanger sequencing were performed in 48 SSc patients and 38 controls. Read More

View Article and Full-Text PDF
October 2021

High-accuracy quantitative principle of a new compact digital PCR equipment: Lab On An Array.

Genomics Inform 2021 Sep 30;19(3):e34. Epub 2021 Sep 30.

Department of Bioconvergence Engineering, Dankook University, Yongin 16890, Korea.

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. Read More

View Article and Full-Text PDF
September 2021

Rolling Circle Amplification in Integrated Microsystems: An Uncut Gem toward Massively Multiplexed Pathogen Diagnostics and Genotyping.

Acc Chem Res 2021 Oct 12. Epub 2021 Oct 12.

Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, 17165 Solna, Sweden.

ConspectusThe development of robust methods allowing the precise detection of specific nucleic acid sequences is of major societal relevance, paving the way for significant advances in biotechnology and biomedical engineering. These range from a better understanding of human disease at a molecular level, allowing the discovery and development of novel biopharmaceuticals and vaccines, to the improvement of biotechnological processes providing improved food quality and safety, efficient green fuels, and smart textiles. Among these applications, the significance of pathogen diagnostics as the main focus of this Account has become particularly clear during the recent SARS-CoV-2 pandemic. Read More

View Article and Full-Text PDF
October 2021

A photofabricated honeycomb micropillar array for loss-free trapping of microfluidic droplets and application to digital PCR.

Lab Chip 2021 10 12;21(20):3933-3941. Epub 2021 Oct 12.

Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, 310023, China.

Droplet microfluidics is a promising platform for various biological and biomedical applications. Among which, droplet-based digital PCR (ddPCR) is one of the most challenging examples, with practical issues involving possible fusion/fission of droplets during PCR thermocycling and difficulties of indexing them for real-time monitoring. While spatially trapped droplet arrays may be helpful, they currently are either of low trapping density or suffer from high droplet loss. Read More

View Article and Full-Text PDF
October 2021

Contagious Ovine Digital Dermatitis: A Novel Bacterial Etiology and Lesion Pathogenesis.

Front Vet Sci 2021 23;8:722461. Epub 2021 Sep 23.

Department of Livestock and One Health, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Neston, United Kingdom.

Contagious ovine digital dermatitis (CODD) is a severe and common infectious foot disease of sheep and a significant animal welfare issue for the sheep industry in the UK and some European countries. The etiology and pathogenesis of the disease are incompletely understood. In this longitudinal, experimental study, CODD was induced in 18 sheep, and for the first time, the clinical lesion development and associated microbiological changes in CODD affected feet are described over time, resulting in a completely new understanding of the etiopathogenesis of CODD. Read More

View Article and Full-Text PDF
September 2021

Detection and differentiation of murine leukemia virus (MLV) and murine stem cell virus (MSCV) and therefrom derived nucleic acids.

J Virol Methods 2021 Oct 7;299:114316. Epub 2021 Oct 7.

Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764, Oberschleißheim, Germany. Electronic address:

Murine leukemia virus (MLV) and murine stem cell virus (MSCV) and derived retroviral vectors are widely used to study retrovirus biology and as tools for gene delivery. The method described here represents a quantitative real time PCR (qPCR) with hydrolysis probe that can be applied within classical qPCR as well as in digital droplet PCR (ddPCR). The method targets a 60 bp long fragment located within the U5 region of the MLV/MSCV genome sequence. Read More

View Article and Full-Text PDF
October 2021

The detection of specific hypermethylated WIF1 and NPY genes in circulating DNA by crystal digital PCR™ is a powerful new tool for colorectal cancer diagnosis and screening.

BMC Cancer 2021 Oct 10;21(1):1092. Epub 2021 Oct 10.

Department of Oncobiology, University Hospital of Besançon, Besançon, France.

Background: In oncology, liquid biopsy is of major relevance from theranostic point of view. The searching for mutations in circulating tumor DNA (ctDNA) in case of colorectal cancers (CRCs) allows the optimization of patient care. In this context, independent of mutation status biomarkers are required for its detection to confirm the presence of ctDNA in liquid biopsies. Read More

View Article and Full-Text PDF
October 2021

Simple, precise, and less-biased GMO quantification by multiplexed genetic element-specific digital PCR.

J AOAC Int 2021 Oct 9. Epub 2021 Oct 9.

Institute of Food Research, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.

Background: To provide the consumer with choices of GMO or non-GMO, official food labeling systems were established in many countries. Because the threshold GMO content values were set to distinguish between "non-GMO" and "GMO" designations, GMO content quantification method are required for ensuring the appropriateness of labeling.

Objective: As the number of GMOs is continuously increasing around the world, we set out to develop a low-cost, simple and less-biased analytical strategy to cover all necessary detection targets. Read More

View Article and Full-Text PDF
October 2021

Campylobacter anatolicus sp. nov., a novel member of the genus Campylobacter isolated from feces of Anatolian Ground Squirrel (Spermophilus xanthoprymnus) in Turkey.

Syst Appl Microbiol 2021 Sep 24;44(6):126265. Epub 2021 Sep 24.

Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Erciyes University, 38280 Kayseri, Turkey.

Seventy-four Gram-negative, motile, slightly curved rod-shaped, microaerophilic, oxidase-positive and catalase-negative isolates, recovered from fecal samples of the Anatolian ground squirrel (Spermophilus xanthoprymnus) in Kayseri, Turkey, were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR indicated that all isolates belonged to the genus Campylobacter. 16S rRNA gene sequence analyses revealed the closest match as Campylobacter curvus DSM 6644 with identity levels of 96. Read More

View Article and Full-Text PDF
September 2021

Treatment-Free Remission: the New Goal in CML Therapy.

Curr Hematol Malig Rep 2021 Oct 7. Epub 2021 Oct 7.

Moffitt Cancer Center, 12902 USF Magnolia Drive, Tampa, FL, 33612, USA.

Purpose Of Review: Treatment-free remission (TFR) is considered one of the main goals of therapy in patients with CML. Our goal in this paper is to review the current data on TFR, and discuss future directions.

Recent Findings: Multiple studies have demonstrated that attempting a treatment-free remission is safe and effective in a select group of patients. Read More

View Article and Full-Text PDF
October 2021

The bioanalytical tools that support cell and gene therapy development.

Authors:
Naamah Maundrell

Bioanalysis 2021 Oct 7. Epub 2021 Oct 7.

Future Science Group, Unitec House, 2 Albert Place, London, N3 1QB, UK.

View Article and Full-Text PDF
October 2021

Viability of sex identification of the blue-fronted Amazon parrot (Amazona aestiva) based on iris color sexual dichromatism.

An Acad Bras Cienc 2021 29;93(suppl 4):e20210060. Epub 2021 Sep 29.

Instituto Multidisciplinar em Saúde, Campus Anísio Teixeira, Universidade Federal da Bahia, Rua Rio de Contas, 58, Candeias, 45029-094 Vitória da Conquista, BA, Brazil.

Reintroducing apprehended wild animal in a natural environment is a complex process that involves many steps, including rehabilitating individuals and ensuring viable populations for reproduction; as such, the proportion between males and females to be reintroduced need to be considered carefully. The need of specialized techniques to identify sex on species that do not present discernible sexual dimorphisms can be a hardship to a successful reintroduction. Amazona aestiva, one of the most apprehended species on Brazil, is an example of such case, as sexing techniques employed for it often involves molecular or surgical procedures. Read More

View Article and Full-Text PDF
October 2021

Sex differences in HIV Persistence and Reservoir Size during Aging.

Clin Infect Dis 2021 Oct 6. Epub 2021 Oct 6.

Case Western Reserve University, Cleveland, OH, United State.

Background: Sex differences in HIV reservoir dynamics remain under-explored.

Methods: Longitudinal samples from virally suppressed midlife women (N=59) and age-matched men (N=31) were analyzed retrospectively. At each time point, we measured sex hormones (by ELISA), cellular HIV DNA and RNA (by digital droplet PCR). Read More

View Article and Full-Text PDF
October 2021

Low-copy transgene detection using nested digital polymerase chain reaction for gene-doping control.

Drug Test Anal 2021 Oct 4. Epub 2021 Oct 4.

Genetic Analysis Department, Laboratory of Racing Chemistry, Utsunomiya, Tochigi, Japan.

Gene doping is prohibited for fair competition in human and horse sports. One style of gene doping is the administration of an exogeneous gene, called a transgene, to postnatal humans and horses. Although many transgene detection methods based on quantitative polymerase chain reaction (PCR), including real-time PCR and digital PCR, have been recently developed, it remains difficult to reliably detect low-copy transgenes. Read More

View Article and Full-Text PDF
October 2021