3,879 results match your criteria differential placental


Placental mitochondrial DNA mutational load and perinatal outcomes: findings from a multi-ethnic pregnancy cohort.

Mitochondrion 2021 Jun 5. Epub 2021 Jun 5.

Department of Environmental Medicine and Public Health, Icahn School of Medicine at Mount Sinai, New York, NY, 10029; Kravis Children's Hospital, Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY, 10029; Institute for Exposomic Research, Icahn School of Medicine at Mount Sinai, New York, NY, 10029.

Mitochondria fuel placental activity, with mitochondrial dysfunction implicated in several perinatal complications. We investigated placental mtDNA mutational load using NextGen sequencing in relation to birthweight and gestational length among 358 mother-newborn pairs. We found that higher heteroplasmy, especially in the hypervariable displacement loop region, was associated with shorter gestational length. Read More

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Identification and validation of DNA methylation changes in pre-eclampsia.

Placenta 2021 Jun 1;110:16-23. Epub 2021 Jun 1.

Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand. Electronic address:

Introduction: Pre-eclampsia (PE) is a dangerous placental condition that can lead to premature labour, seizures and death of mother and infant. Several studies have identified altered placental DNA methylation in PE; however, there is widespread inconsistency between studies and most findings have not been replicated. This study aimed to identify and validate consistent differences in methylation across multiple PE cohorts. Read More

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Anandamide Exerts a Differential Effect on Human Placenta Before and After the Onset of Labor.

Front Physiol 2021 21;12:667367. Epub 2021 May 21.

Laboratorio de Fisiopatología Placentaria, Facultad de Ciencias Médicas, Centro de Estudios Farmacológicos y Botánicos (CEFyBO - CONICET - UBA), Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.

The onset of labor involves the action of multiple factors and recent reports have postulated the endocannabinoid system as a new regulator of this process. Our objective was to study the role of anandamide, one of the main endocannabinoids, on the regulation of placental molecules that contribute to the onset of labor at term. Placental samples were obtained from patients with laboring vaginal deliveries and from non-laboring elective cesarean sections. Read More

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Differential methylation of genes in the human placenta associated with bisphenol A exposure.

Environ Res 2021 Jun 2;200:111389. Epub 2021 Jun 2.

Department of Epidemiology and Social Science, NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, China.

Prenatal exposure to bisphenol A (BPA) is associated with numerous adverse health outcomes among offspring. Although DNA methylation is considered one of the underlying causes of these associations, few studies have focused on the association between prenatal BPA exposure and DNA methylation in the human placenta. In this study, we examined the association between prenatal BPA exposure and DNA methylation in the placenta of 146 mother-infant pairs from the Shanghai-Minhang Birth Cohort Study. Read More

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Whole Transcriptome Analysis: Implication to Estrous Cycle Regulation.

Biology (Basel) 2021 May 25;10(6). Epub 2021 May 25.

College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.

Estrous cycle is one of the placental mammal characteristics after sexual maturity, including estrus stage (ES) and diestrus stage (DS). Estrous cycle is important in female physiology and its disorder may lead to diseases, such as polycystic ovary syndrome, ovarian carcinoma, anxiety, and epilepsy. In the latest years, effects of non-coding RNAs and messenger RNA (mRNA) on estrous cycle have started to arouse much concern, however, a whole transcriptome analysis among non-coding RNAs and mRNA has not been reported. Read More

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Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of .

Int J Mol Sci 2021 May 13;22(10). Epub 2021 May 13.

Grupo de Bioquímica y Parasitología Molecular (CTS 183), Departamento de Parasitología, Campus de Fuentenueva, Instituto de Biotecnología, Universidad de Granada, 18071 Granada, Spain.

Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypomastigotes (mammalian infective stage; (TCT)) and epimastigotes (insect stage; (E)) of , the etiologic agent of Chagas disease. EVs of each stage were isolated by differential centrifugation and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS), dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), electron microscopy and atomic force microscopy (AFM). Read More

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Differential Alveolar and Systemic Oxygenation during Preterm Resuscitation with 100% Oxygen during Delayed Cord Clamping.

Am J Perinatol 2021 Jun 1. Epub 2021 Jun 1.

Neonatal Research Institute, Sharp Mary Birch Hospital for Women and Newborns, San Diego, California.

Objective:  Delayed cord clamping (DCC) and 21 to 30% O resuscitation is recommended for preterm infants but is commonly associated with low pulmonary blood flow (Qp) and hypoxia. 100% O supplementation during DCC for 60 seconds followed by 30% O may increase Qp and oxygen saturation (SpO).

Study Design:  Preterm lambs (125-127 days of gestation) were resuscitated with 100% O with immediate cord clamping (ICC,  = 7) or ICC + 30% O, and titrated to target SpO ( = 7) or DCC + 100% O for 60 seconds, which followed by cord clamping and 30% O titration ( = 7). Read More

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A cross-cohort analysis of autosomal DNA methylation sex differences in the term placenta.

Biol Sex Differ 2021 May 27;12(1):38. Epub 2021 May 27.

BC Children's Hospital Research Institute, 950 W 28th Ave, Vancouver, V6H 3N1, Canada.

Background: Human placental DNA methylation (DNAme) data is a valuable resource for studying sex differences during gestation, as DNAme profiles after delivery reflect the cumulative effects of gene expression patterns and exposures across gestation. Here, we present an analysis of sex differences in autosomal DNAme in the uncomplicated term placenta (n = 343) using the Illumina 450K array.

Results: At a false discovery rate < 0. Read More

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Neonatal thrombocytopenia: A review. I. Definitions, differential diagnosis, causes, immune thrombocytopenia.

Authors:
Hugo Donato

Arch Argent Pediatr 2021 06;119(3):e202-e214

Consultorios Privados de Hematología Infantil. Ciudad Autónoma de Buenos Aires, Argentina.

Thrombocytopenia, defined as a platelet count below 100 x 109/L, is a very common finding in the neonatal period, especially in critically ill infants and preterm newborns. Its causes are multiple: it may be due both to pediatric conditions and to other factors involved in the fetal-placental-maternal interface. This initial article describes the causes of thrombocytopenia, proposes a diagnostic approach to manage a thrombocytopenic newborn infant, and provides a detailed description of the different conditions corresponding to thrombocytopenia of immune etiology. Read More

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Twin anemia polycythemia sequence in dichorionic diamniotic twins: A case report and review of the literature.

Clin Case Rep 2021 May 15;9(5):e04184. Epub 2021 May 15.

Department of Obstetrics and Gynaecology Westmead Hospital Westmead NSW Australia.

Although rare, literature demonstrates evidence that vascular anastomoses do occur in dichorionic twins. Therefore, twin anemia polycythemia sequence should be considered as a differential diagnoses in dichorionic twins if there is suspicion on antenatal ultrasound. Read More

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Effects of antiseizure medications on placental cells: Focus on heterodimeric placental carriers.

Epilepsy Res 2021 May 8;174:106664. Epub 2021 May 8.

Institute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel. Electronic address:

Objective: Appropriate placental nutrient transfer is essential for optimal fetal development. We have previously shown that antiseizure medications (ASMs) can alter the expression of placental carriers for folate and thyroid hormones. Here we extended our analysis to heterodimeric carriers that mediate the placental uptake of amino acids and antioxidant precursors. Read More

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Network analysis reveals important genes in human placenta.

J Obstet Gynaecol Res 2021 May 18. Epub 2021 May 18.

Department of Gynaecology and Obstetrics, Fuzhou First Hospital Affiliated to Fujian Medical University, Fuzhou, China.

Aim: To determine which genes are important in placenta by network analysis.

Methods: Placenta expressing genes were screened from RNA-Seq data. Protein-protein interaction data were downloaded from STRING (v11. Read More

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Differential Expression of CB Cannabinoid Receptor and Cannabinoid Receptor Interacting Protein 1a in Labor.

Cannabis Cannabinoid Res 2021 Apr 16. Epub 2021 Apr 16.

Department of Physiology and Pharmacology, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.

The endocannabinoid system is present in multiple organ systems and is involved in smooth muscle regulation, immune function, neuroendocrine modulation, and metabolism of tissues. Limited data are available regarding the presence and role of this system in reproductive tissues. Components of the endocannabinoid system have been identified in myometrial and placental tissues. Read More

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Considering intrauterine location in a model of fetal growth restriction after maternal titanium dioxide nanoparticle inhalation.

Front Toxicol 2021 Mar 23;3. Epub 2021 Mar 23.

Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy, Rutgers University, 160 Frelinghuysen Rd., Piscataway, NJ 08854, USA.

Fetal growth restriction (FGR) is a condition with several underlying etiologies including gestational disease (e.g., preeclampsia, gestational diabetes) and xenobiotic exposure (e. Read More

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Postnatally induced metabolic and oxidative changes associated with maternal high-fat consumption were mildly affected by Quercetin-3-O-rutinoside treatment in rats.

Heliyon 2021 Apr 28;7(4):e06847. Epub 2021 Apr 28.

Discipline of Human Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban, 4000, South Africa.

Oxidative stress is usually associated with prolonged intake of high-fat diet (HFD). However, little is known about the impact of maternal HFD on endogenous modulation of antioxidant-defence-enzyme-network, its link to adverse fetal growth and overall effects of Quercetin-3-o-rutinoside (QR) supplementation. Sprague-Dawley rats were initially assigned to normal diet (ND) or HFD for 8 weeks and mated. Read More

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Conservative treatment for hypervascularised placental polyp with secondary haemoperitoneum: a case report.

Acta Biomed 2021 04 30;92(S1):e2021157. Epub 2021 Apr 30.

Department of Medicine and Surgery, Unit of Surgical Sciences, Obstetrics and Gynecology University of Parma.

Objective: We describe the first case to our knowledge of Hypervascularised placental polyp (HPP) presenting with acute pelvic pain and hemoperitoneum.

Case Report: A 33 years-old woman with a history of medical abortion three months earlier came to our attention complaining acute pelvic pain and vaginal bleeding. Transvaginal (TV) and transabdominal (TA) ultrasound (US) demonstrated a highly vascular intrauterine lesion and intra-abdominal free fluid consistent with a diagnosis of haemoperitoneum. Read More

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Fetal hypoxia and apoptosis following maternal porcine reproductive and respiratory syndrome virus (PRRSV) infection.

BMC Vet Res 2021 May 1;17(1):182. Epub 2021 May 1.

Western College of Veterinary Medicine, Saskatoon, 52 Campus Dr, Saskatoon, Saskatchewan, S7N 5B4, Canada.

Background: Mechanisms of fetal death following maternal PRRSV2 infection remain uncharacterized, although hypoxia from umbilical cord lesions and/or placental detachment due to apoptosis are hypothesized. We performed two experiments examining hypoxia and apoptosis in PRRSV-infected and non-infected, third-trimester fetuses to elucidate possible associations with fetal death. Fetuses were selected based on four phenotypic infection groups: fetuses from non-challenged control gilts (CTRL); low viral load fetuses (LVL; Exp 1) or uninfected fetuses (UNINF; Exp 2) from inoculated gilts; viable high viral load fetuses (HVL-VIA); and HVL meconium-stained fetuses (HVL-MEC). Read More

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Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs.

Int J Mol Sci 2021 Apr 16;22(8). Epub 2021 Apr 16.

Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, VIC 3010, Australia.

Placental insufficiency is a known consequence of maternal heat stress during gestation in farm animals. The molecular regulation of placentae during the stress response is little known in pigs. This study aims to identify differential gene expression in pig placentae caused by maternal heat exposure during early to mid-gestation. Read More

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Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR).

Cells 2021 Apr 16;10(4). Epub 2021 Apr 16.

Department of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, Poland.

Impaired fetal growth is one of the most important causes of prematurity, stillbirth and infant mortality. The pathogenesis of idiopathic fetal growth restriction (FGR) is poorly understood but is thought to be multifactorial and comprise a range of genetic causes. This research aimed to investigate non-coding RNAs (lncRNAs) in the placentas of male and female fetuses affected by FGR. Read More

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Anterior Gradient Protein 3 and S100 Calcium-Binding Protein P Levels in Different Endometrial Epithelial Compartments May Play an Important Role in Recurrent Pregnancy Failure.

Int J Mol Sci 2021 Apr 7;22(8). Epub 2021 Apr 7.

Centre for Women's Health Research, Department of Women's and Children's Health, Institute of Life Course and Medical Sciences, University of Liverpool, Member of Liverpool Health Partners, Liverpool L8 7SS, UK.

Recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) are distressing conditions without effective treatments. The luminal epithelium (LE) is integral in determining receptivity of the endometrium, whereas functionalis glands and stroma aid in nurturing early embryo development. Calcium signalling pathways are known to be of vital importance to embryo implantation and pregnancy establishment, and anterior gradient protein 3 (AGR3) and S100 calcium-binding protein P (S100P) are involved with these pathways. Read More

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Cyanidin 3-glucoside modulated cell cycle progression in liver precancerous lesion, study.

World J Gastroenterol 2021 Apr;27(14):1435-1450

Department of Clinical Pharmacology, Ain Shams Faculty of Medicine, Cairo 11381, Egypt.

Background: Cyanidin-3-O-glucoside (cyan) exhibits antioxidant and anticancer properties. The cell cycle proteins and antimitotic drugs might be promising therapeutic targets in hepatocellular carcinoma.

Aim: To investigate the effect of cyan administration on cell cycle in hepatic precancerous lesion (PCL) induced by diethylnitrosamine/2-acetylaminofluorene (DEN/2-AAF) in Wistar rats. Read More

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Increased homocysteine mediated oxidative stress as key determinant of hepatitis E virus (HEV) infected pregnancy complication and outcome: A study from Northeast India.

Infect Genet Evol 2021 Apr 24;92:104882. Epub 2021 Apr 24.

Department of Biotechnology, Gauhati University, Guwahati, Assam 781014, India. Electronic address:

With the background of association of oxidative stress and Hepatitis E virus (HEV) infection in pregnancy complications the present novel study aimed to evaluate the significance of changes in maternal homocysteine levels and the related mechanism(s) in the pathophysiology of HEV related pregnancy complications and negative outcomes. Term delivery (TD, N = 194) and HEV-IgM positive pregnancy cases [N = 109] were enrolled. Serum and placental homocysteine levels were evaluated by ELISA and immunofluorescence and in turn correlated with serum Vitamin B12 levels. Read More

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Glutathione and oxidized nicotinamide adenine dinucleotide (NAD) redox status in plasma and placental tissue of Saudi patients with intrauterine growth restriction.

Saudi Med J 2021 May;42(5):491-498

From the Department of Clinical Laboratory Sciences and the Chair of Medical and Molecular Genetics Research (Aljaser, Ghneim, Abudawood, Fatima, ALsheikh), College of Applied Medical Sciences, King Saud University; from the Department of Obstetrics and Gynecology (ALshubaily), College of Medicine, King Saud University; from the Department of Clinical Laboratory Sciences (Almajed), College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Kingdom of Saudi Arabia.

Objectives: To assess the oxidation state and gene expression profiles of relevant enzymes in intrauterine growth restriction (IUGR) patients in Saudi Arabia.

Methods: Current case-control study involved plasma and placental tissue samples from 25 IUGR patients and 25 healthy pregnant (HP) women attending the Obstetrics and Gynecology Clinic, King Khalid University Hospital, Riyadh, Saudi Arabia, between April and November 2017. We compared hydrogen peroxide, superoxide anions, malondialdehyde, and oxidative stress markers levels and the activities of glutathione-related enzymes (glutathione peroxidase [GPx], glutathione reductase [GR], glutathione S-transferase [GST], glutamate cysteine ligase [GCL], glutathione synthetase [GS], reduced glutathione [GSH], oxidized glutathione [GSSG], and oxidized nicotinamide adenine dinucleotide [NAD], and reduced NAD [NADH]) between the 2 groups. Read More

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Expression of tissue factor and thrombomodulin in the placentas of pregnancies affected by early-onset and late-onset preeclampsia.

J Obstet Gynaecol Res 2021 Apr 19. Epub 2021 Apr 19.

Department of Pathology. Faculty of Medicine, Pontificia Universidad Javeriana, Hospital Universitario San Ignacio, Bogotá, Colombia.

Aim: To analyze the differential genetic expression and protein localization of thrombomodulin (THBD) and tissue factor (F3) in the placentas of pregnancies affected by preeclampsia.

Methods: We assessed the expression of THBD and F3 by immunohistochemistry and real-time polymerase chain reaction (PCR) in placentas from 20 PE cases: 10 early-onset PE placentas, 10 late-onset PE placentas, and 10 control cases (normal pregnancies).

Results: In cases, we found higher THBD and F3 RNA levels in placental tissue. Read More

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Pulmonary metastasis as a primary manifestation of gestational choriocarcinoma in a third trimester pregnancy.

Gynecol Oncol Rep 2021 May 31;36:100762. Epub 2021 Mar 31.

Wayne State University School of Medicine, Department of Obstetrics and Gynecology, 3980 John R, 7-Brush N, MB #165, Detroit, MI 48201, USA.

•Choriocarcinomas can follow molar, ectopic, or normal pregnancies.•The early diagnosis and treatment of choriocarcinomas is imperative.•Atypical symptoms in pregnancy should raise suspicion for choriocarcinoma. Read More

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Differential Transcriptomes and Methylomes of Trophoblast Stem Cells From Naturally-Fertilized and Somatic Cell Nuclear-Transferred Embryos.

Front Cell Dev Biol 2021 1;9:664178. Epub 2021 Apr 1.

Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.

Trophoblast stem cells (TSCs) are critical to mammalian embryogenesis by providing the cell source of the placenta. TSCs can be derived from trophoblast cells. However, the efficiency of TSC derivation from somatic cell nuclear transfer (NT) blastocysts is low. Read More

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Identification of methylation changes associated with positive and negative growth deviance in Gambian infants using a targeted methyl sequencing approach of genomic DNA.

FASEB Bioadv 2021 Apr 5;3(4):205-230. Epub 2021 Feb 5.

Department of Pathology University of Cambridge Cambridge UK.

Low birthweight and reduced height gain during infancy (stunting) may arise at least in part from adverse early life environments that trigger epigenetic reprogramming that may favor survival. We examined differential DNA methylation patterns using targeted methyl sequencing of regions regulating gene activity in groups of rural Gambian infants: (a) low and high birthweight (DNA from cord blood ( = 16 and  = 20, respectively), from placental trophoblast tissue ( = 21 and  = 20, respectively), and DNA from peripheral blood collected from infants at 12 months of age ( = 23 and  = 17, respectively)), and, (b) the top 10% showing rapid postnatal length gain (high,  = 20) and the bottom 10% showing slow postnatal length gain (low,  = 20) based on z score change between birth and 12 months of age (LAZ) (DNA from peripheral blood collected from infants at 12 months of age). Using BiSeq analysis to identify significant methylation marks, for birthweight, four differentially methylated regions (DMRs) were identified in trophoblast DNA, compared to 68 DMRs in cord blood DNA, and 54 DMRs in 12-month peripheral blood DNA. Read More

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Differential expression of mTOR related molecules in the placenta from gestational diabetes mellitus (GDM), intrauterine growth restriction (IUGR) and preeclampsia patients.

Reprod Biol 2021 Jun 4;21(2):100503. Epub 2021 Apr 4.

Lung and Placenta Research Laboratory, Brigham Young University, Department of Physiology and Developmental Biology, Provo, UT, USA. Electronic address:

The mechanistic target of rapamycin (mTOR) pathway is involved in the function and growth of the placenta during pregnancy. The mTOR pathway responds to nutrient availability and growth factors that regulate protein expression and cell growth. Disrupted mTOR signaling is associated with the development of several obstetric complications. Read More

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: an emerging pathogen in female reproductive disease and adverse perinatal outcomes.

Crit Rev Microbiol 2021 Apr 6:1-26. Epub 2021 Apr 6.

Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U. S. Department of Health and Human Services, Detroit, MI, USA.

is an emerging pathogen implicated in adverse reproductive and perinatal outcomes. Although scarce, recent data suggest that vaginally residing becomes pathogenic following its ascension into the upper urogenital tract, amniotic fluid, placenta, and foetal membranes. The role of in women's health and disease is generally underappreciated because the cultivation of these bacteria is limited by their complex nutritional requirements, slow growth patterns, and anaerobic nature. Read More

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Mechanobiology-informed regenerative medicine: Dose-controlled release of placental growth factor from a functionalized collagen-based scaffold promotes angiogenesis and accelerates bone defect healing.

J Control Release 2021 Jun 1;334:96-105. Epub 2021 Apr 1.

Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland; Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland; Advanced Materials and Bioengineering Research Centre (AMBER), Royal College of Surgeons in Ireland and Trinity College Dublin, Dublin, Ireland. Electronic address:

Leveraging the differential response of genes to mechanical loading may allow for the identification of novel therapeutics and we have recently established placental growth factor (PGF) as a mechanically augmented gene which promotes angiogenesis at higher doses and osteogenesis at lower doses. Herein, we sought to execute a mechanobiology-informed approach to regenerative medicine by designing a functionalized scaffold for the dose-controlled delivery of PGF which we hypothesized would be capable of promoting regeneration of critically-sized bone defects. Alginate microparticles and collagen/hydroxyapatite scaffolds were shown to be effective PGF-delivery platforms, as demonstrated by their capacity to promote angiogenesis in vitro. Read More

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