7,652,239 results match your criteria diagnostic testing


Dilated Cardiomyopathy Associated with Paraquat Herbicide Poisoning.

Clin Pract 2021 Sep 18;11(3):679-686. Epub 2021 Sep 18.

Department of Cardiology, Nassau University Medical Center, East Meadow, NY 11554, USA.

Dilated cardiomyopathy is a subset of cardiomyopathies defined by reduced ejection fraction of less than 45% and a dilated left ventricle. While dilated cardiomyopathy is common, its etiology is not always readily evident. Paraquat is used as an herbicide worldwide and is one of the main causes of fatal poisoning in underdeveloped countries in Asia, Central America, and the Pacific Islands. Read More

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September 2021

Diagnostic and Therapeutic Challenges of Cerebral Venous Thrombosis in SARS-CoV-2 Infection: A Case Report and Review of Literature.

Clin Pract 2021 Sep 7;11(3):598-606. Epub 2021 Sep 7.

School of Medicine, Caribbean Medical University, 4797 Willemstad, Curaçao.

Headache, a common prodromal symptom of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, can also be a manifestation of cerebral venous thrombosis (CVT), secondary to COVID-19. CVT management continues to evolve, with direct oral anticoagulants (DOACs) emerging as an alternative to warfarin. A 44-year-old Asian female, with no past medical history, presented to the emergency room (ER) with complaints of nonproductive cough and left-sided headache. Read More

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September 2021

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19.

Biosensors (Basel) 2021 Aug 25;11(9). Epub 2021 Aug 25.

Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.

The development of reliable and robust diagnostic tests is one of the most efficient methods to limit the spread of coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, most laboratory diagnostics for COVID-19, such as enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR), are expensive, time-consuming, and require highly trained professional operators. On the other hand, the lateral flow immunoassay (LFIA) is a simpler, cheaper device that can be operated by unskilled personnel easily. Read More

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A novel droplet digital polymerase chain reaction for diagnosis of pneumocystis pneumonia (PCP)-a clinical performance study and survey of sulfamethoxazole-trimethoprim resistant mutations.

J Infect 2021 Sep 22. Epub 2021 Sep 22.

Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Electronic address:

Objectives: To determine the performance of droplet digital polymerase chain reaction (ddPCR) assays in diagnosing pneumocystis pneumonia (PCP), and to survey the sulfamethoxazole-trimethoprim (SMX-TMP) resistant mutations in our PCP cohort.

Methods: A prospective study was conducted from January 2017 to June 2018. Adult immunocompromised subjects with pneumonia were enrolled. Read More

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September 2021

Increasing diagnostic possibilities using the geneLEAD VIII platform for detection of SARS-CoV-2.

J Virol Methods 2021 Sep 22:114291. Epub 2021 Sep 22.

Department of Medical Microbiology, Leiden University Medical Center, 2300 RC, Leiden, the Netherlands.

At the time SARS-CoV-2 was identified as the cause of coronavirus disease 2019 (COVID-19) no in vitro diagnostic (IVD) tests were available since it was a new virus. Very shortly after the release of the genomic sequence of SARS-CoV-2, laboratory-developed tests (LDTs) were developed, made available and implemented in several laboratories in the Netherlands and globally. In this study, the performance of an E-gene Sarbeco specific real-time reverse-transcriptase PCR (RT-PCR) was verified on the open modus of the geneLEAD VIII sample-to-answer platform. Read More

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September 2021

Randomized pilot trial on optimal treatment strategy, myocardial changes, and prognosis of patients with myocardial infarction with non-obstructive coronary arteries (MINOCA).

Am J Med 2021 Sep 22. Epub 2021 Sep 22.

Clinic of Heart and Vessel Diseases, Institute of Clinical Medicine Faculty of Medicine, Vilnius University, Vilnius, Lithuania.

Background: Myocardial infarction with non-obstructive coronary arteries (MINOCA) remains an unresolved challenge. Many different diagnostic approaches are often required to diagnose, confirm, and evaluate MINOCA. The prevalence can be as high as 13 % of all acute MI patients indicating that this condition is not rare. Read More

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September 2021

Weakly supervised annotation-free cancer detection and prediction of genotype in routine histopathology.

J Pathol 2021 Sep 24. Epub 2021 Sep 24.

Department of Medicine III, University Hospital RWTH Aachen, Aachen, Germany.

Deep Learning is a powerful tool in computational pathology: it can be used for tumor detection and for predicting genetic alterations based on histopathology images alone. Conventionally, tumor detection and prediction of genetic alterations are two separate workflows. Newer methods have combined them, but require complex, manually engineered computational pipelines, restricting reproducibility and robustness. Read More

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September 2021

Understanding diagnostic processes in emergency departments: a mixed methods case study protocol.

BMJ Open 2021 Sep 24;11(9):e044194. Epub 2021 Sep 24.

Emergency Medicine and Pediatrics, University of Michigan, Ann Arbor, Michigan, USA.

Introduction: Diagnostic processes in the emergency department (ED) involve multiple interactions among individuals who interface with information systems to access and record information. A better understanding of diagnostic processes is needed to mitigate errors. This paper describes a study protocol to map diagnostic processes in the ED as a foundation for developing future error mitigation strategies. Read More

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September 2021

Genetics of craniofacial malformations.

Semin Fetal Neonatal Med 2021 Sep 17:101290. Epub 2021 Sep 17.

Institute of Human Genetics, Medical Faculty and University Hospital Düsseldorf, Heinrich-Heine-University Düsseldorf, Germany. Electronic address:

The field of craniofacial malformations is comprehensive and does not allow to discuss all craniofacial malformations which have been described as single entities. Many of the syndromes with craniofacial malformations are ultrarare. In this review we have chosen craniofacial malformation syndromes which are of relevance for the pediatrician, especially neonatologist: different types of craniosynostoses, oculo-auriculo-vertebral spectrum, Pierre Robin sequence and Treacher Collins syndrome. Read More

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September 2021

AUR-RRA Review: Logistics of Academic-Industry Partnerships in Artificial Intelligence.

Acad Radiol 2021 Sep 21. Epub 2021 Sep 21.

Department of Radiology and Radiological Sciences, University of Utah, 1950 Circle of Hope - 3rd floor Breast Imaging Clinic, Salt Lake City, UT 84112. Electronic address:

The Radiology Research Alliance (RRA) of the Association of University Radiologists (AUR) convenes Task Forces to address current topics in radiology. In this article, the AUR-RRA Task Force on Academic-Industry Partnerships for Artificial Intelligence, considered issues of importance to academic radiology departments contemplating industry partnerships in artificial intelligence (AI) development, testing and evaluation. Our goal was to create a framework encompassing the domains of clinical, technical, regulatory, legal and financial considerations that impact the arrangement and success of such partnerships. Read More

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September 2021

Acceptance and perceived value of non-invasive malaria diagnostic tests in malaria-endemic countries.

Malar J 2021 Sep 24;20(1):379. Epub 2021 Sep 24.

FIND, Geneva, Switzerland.

Background: The diagnosis of malaria, using microscopy or rapid diagnostic tests (RDTs), requires the collection of capillary blood. This procedure is relatively simple to perform but invasive and poses potential risks to patients and health workers, arising from the manipulation of potentially infectious bodily fluids. Less or non-invasive diagnostic tests, based on urine, saliva or requiring no sampling, have the potential to generate less discomfort for the patient and to offer simpler and less risky testing procedures that could be safely performed by untrained staff or even self-performed. Read More

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September 2021

"That was quick, simple, and easy": Patient perceptions of acceptability of point-of-care hepatitis C RNA testing at a reception prison.

Int J Drug Policy 2021 Sep 21;99:103456. Epub 2021 Sep 21.

The Kirby Institute, UNSW Sydney, Level 6, Wallace Wurth Building, Sydney, NSW, 2052, Australia.

Introduction: Current diagnostic pathways require multiple healthcare provider visits and lead to a drop-off in the hepatitis C virus (HCV) testing and treatment care cascade. In prison settings, frequent transitioning between prisons and the community further reduces uptake of testing and treatment. The PIVOT study evaluated a 'one-stop-shop' intervention integrating point-of-care HCV RNA testing, Fibroscan®-based liver disease assessment, and treatment prescription at a reception prison in Australia. Read More

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September 2021

Artificial intelligence for quality control of oscillometry measures.

Comput Biol Med 2021 Sep 14;138:104871. Epub 2021 Sep 14.

Department of Electronic, Information and Bioengineering, Politecnico di Milano University, Milan, Italy.

Background: The forced oscillation technique (FOT) allows non-invasive lung function testing during quiet breathing even without expert guidance. However, it still relies on an operator for excluding breaths with artefacts such as swallowing, glottis closure and coughing. This manual selection is operator-dependent and time-consuming. Read More

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September 2021

Comparing the diagnostic accuracy of rapid antigen detection tests to real time polymerase chain reaction in the diagnosis of SARS-CoV-2 infection: A systematic review and meta-analysis.

J Clin Virol 2021 Sep 16;144:104985. Epub 2021 Sep 16.

Department of Preventive Medicine, Korea University College of Medicine, Seoul, Republic of Korea.

Background: Timely and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is crucial to reduce the risk of viral transmission. We investigated the diagnostic accuracy of rapid antigen detection tests (RADTs) in the diagnosis of SARS-CoV-2 infection.

Methods: A systematic literature search was performed using Pubmed, Embase, and the Cochrane Central Register. Read More

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September 2021

Loop-Mediated Isothermal Amplification assays for on-site detection of the main sweetpotato infecting viruses.

J Virol Methods 2021 Sep 21:114301. Epub 2021 Sep 21.

International Potato Center, Avenida La Molina 1895, La Molina, Apartado Postal 1558, Lima, Peru. Electronic address:

Globally, Sweet potato feathery mottle virus (SPFMV) and Sweet potato chlorotic stunt virus (SPCSV) occur frequently and in combination cause sweetpotato virus disease (SPVD). Many viral diseases are economically important and negatively impact the production and movement of germplasm across regions. Rapid detection of viruses is critical for effective control. Read More

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September 2021

Clinical and Pathologic Features Associated With Invasive Breast Carcinoma with 2018 American Society of Clinical Oncology/College of American Pathologists In Situ Hybridization Group 2 Results (Human Epidermal Growth Factor Receptor 2 [HER2]/Chromosome 17 Centromere [CEP17] Ratio ≥2.0 and Average HER2 Copy Number <4.0).

Arch Pathol Lab Med 2021 Sep 27. Epub 2021 Sep 27.

From the Robert J Tomsich Pathology & Laboratory Medicine Institute (Hoda, McIntire, Sprague, Komforti, Downs-Kelly).

Context.—: The American Society of Clinical Oncology/College of American Pathologists updated the human epidermal growth factor receptor 2 (HER2) breast carcinoma testing guideline in 2018 to address issues from uncommon HER2 fluorescence in situ hybridization (FISH) results. Based on the 2013 American Society of Clinical Oncology/College of American Pathologists guideline, cases wherein the HER2/chromosome 17 centromere (CEP17) ratio of 2. Read More

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September 2021

Analyses of Original and Computationally-Derived Electronic Health Record Data: The National COVID Cohort Collaborative.

J Med Internet Res 2021 Sep 12. Epub 2021 Sep 12.

Department of Biomedical and Medical Education, School of Medicine, University of Washington, Seattle, US.

Background: Computationally-derived ("synthetic") data can enable the creation and analysis of clinical, laboratory, and diagnostic data as if they were the original electronic health record (EHR) data. Synthetic data can support data sharing to answer critical research questions to address the COVID-19 pandemic.

Objective: We aimed to (1) compare the results from analyses of synthetic data to those from original data, and (2) assess the strengths and limitations of leveraging computationally-derived data for research purposes. Read More

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September 2021

Evaluation of venous plasma glucose measured by point-of-care testing (Accu-Chek Inform II) and a hospital laboratory hexokinase method (Cobas c701) in oral glucose tolerance testing during pregnancy - a challenge in diagnostic accuracy.

Scand J Clin Lab Invest 2021 Sep 24:1-8. Epub 2021 Sep 24.

Department of Obstetrics and Gynecology, and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden.

To diagnose gestational diabetes mellitus (GDM), plasma glucose measurements during oral glucose tolerance test (OGTT) put high demands on the methods in terms of accuracy. The aim was to evaluate and compare diagnostic performance of a point-of-care test and a glucose hexokinase laboratory method. Using risk-based screening, 175 pregnant women were included. Read More

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September 2021

Mutations at a split codon in the GTPase-encoding domain of OPA1 cause dominant optic atrophy through different molecular mechanisms.

Hum Mol Genet 2021 Sep 24. Epub 2021 Sep 24.

Department of Neurosciences, Biomedicine and Movement Sciences, Section of Biological Chemistry, University of Verona, Verona 37134, Italy.

Exonic (i.e. coding) variants in genes associated with disease can exert pathogenic effects both at the protein and mRNA level, either by altering the amino acid sequence or by affecting pre-mRNA splicing. Read More

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September 2021

MRP8/14 serum levels as diagnostic markers for systemic juvenile idiopathic arthritis in children with prolonged fever.

Rheumatology (Oxford) 2021 Sep 24. Epub 2021 Sep 24.

Department of Pediatric Hematology-Oncology University of Duisburg-Essen, Essen, Germany.

Objectives: Differential diagnosis in children with prolonged fever is challenging. In particular, differentiating systemic-onset juvenile idiopathic arthritis (SJIA) from infectious diseases is difficult. Biomarkers are needed supporting the diagnostic work-up. Read More

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September 2021

Perfusion and Permeability MRI Predicts Future Cavernous Angioma Hemorrhage and Growth.

J Magn Reson Imaging 2021 Sep 24. Epub 2021 Sep 24.

Neurovascular Surgery Program, Department of Neurosurgery, University of Chicago Medicine and Biological Sciences, Chicago, Illinois, USA.

Background: Cerebral cavernous angioma (CA) is a capillary vasculopathy affecting more than a million Americans with a small fraction of cases demonstrating lesional bleed or growth with major clinical sequelae. Perfusion and permeability are fundamental features of CA pathophysiology, but their role as prognostic biomarkers is unclear.

Purpose: To investigate whether perfusion or permeability lesional descriptors derived from dynamic contrast-enhanced quantitative perfusion (DCEQP) magnetic resonance imaging (MRI) can predict subsequent lesional bleed/growth in the year following imaging. Read More

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September 2021

Evaluation of the Sysmex XN-31 automated analyser for blood donor malaria screening at Malawi Blood Transfusion Services.

Vox Sang 2021 Sep 23. Epub 2021 Sep 23.

Sysmex Europe, Norderstedt, Germany.

Background And Objectives: Balancing blood supply safety and sufficiency is challenging in malaria-endemic countries where the risk of transfusion-transmitted malaria (TTM) is ever-present. In support of reducing this risk, our study aimed at evaluating the performance of the Sysmex XN-31 analyser in blood donor malaria screening, as compared with current practice in Malawi.

Materials And Methods: This prospective observational study was conducted on remnant venous donor blood samples collected at Malawi Blood Transfusion Service donation sites countrywide for routine blood-borne pathogen screening. Read More

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September 2021

Antimicrobial resistance among , , and spp. isolates from clinical specimens from a hospital in Nairobi, Kenya.

PeerJ 2021 1;9:e11958. Epub 2021 Sep 1.

Biomedical and Diagnostic Sciences, University of Tennessee, Knoxville, TN, United States of America.

Background: Antimicrobial resistance among pathogens of public health importance is an emerging problem in sub-Saharan Africa. Unfortunately, published information on the burden and patterns of antimicrobial resistance (AMR) in this region is sparse. There is evidence that the burden and patterns of AMR vary by geography and facility. Read More

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September 2021

Use of a new multiplex quantitative polymerase chain reaction based assay for simultaneous detection of , , and .

Iran J Microbiol 2021 Aug;13(4):464-469

Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.

Background And Objectives: and cause 90% of bacterial meningitis. Almost all infected people die or have irreversible neurological complications. Therefore, it is essential to have a diagnostic kit with the ability to quickly detect these fatal infections. Read More

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Decreased Autonomic Reactivity and Psychiatric Comorbidities in Neurological Patients With Medically Unexplained Sensory Symptoms: A Case-Control Study.

Front Neurol 2021 7;12:713391. Epub 2021 Sep 7.

Department of Psychosomatic Medicine and Psychotherapy, University Medical Hospital Tübingen, Tübingen, Germany.

Up to 48% of patients with medically unexplained symptoms seen in neurological practice suffer from sensory symptoms, which could be of functional nature or secondary to psychiatric disorders. These patients show high medical care utilization causing elevated healthcare costs. Despite the high prevalence, little is known about clinical characteristics and pathophysiological mechanisms. Read More

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September 2021

Research on the Cognitive Diagnosis of Chinese Listening Comprehension Ability Based on the G-DINA Model.

Front Psychol 2021 7;12:714568. Epub 2021 Sep 7.

Collaborative Innovation Center of Assessment for Basic Education Quality, Beijing Normal University, Beijing, China.

As a new generation of measurement theory, cognitive diagnosis theory shows significant potential and advantages in educational evaluation in that it combines a cognitive process and a measurement method. The application of the theory not only reveals the potential characteristics of learners in cognitive processing, but also provides targeted remedies and strategic guidance for individuals. Given the difficulties of traditional assessment models in providing an insightful and fine-grained account for individualized and procedural learning, providing personalized learning strategies for learners of Chinese as a second language has been a new goal of teaching and measurement in Chinese listening. Read More

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September 2021

Adrenal steroids reference ranges in infancy determined by LC-MS/MS.

Pediatr Res 2021 Sep 23. Epub 2021 Sep 23.

Department of Pediatric Endocrinology and Diabetes, School of Medicine, Marmara University, Istanbul, Turkey.

Background: Interpretation of the results of steroid hormone measurements is challenging at early infancy. The liquid chromatography-tandem mass spectrometry (LC-MS/MS) method provides a powerful tool for diagnosing steroidogenesis disorders. We aimed to develop normative data for a 14-steroid panel and four adrenal enzyme activity indices, determined by LC-MS/MS from 3 days to 6 months of age. Read More

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September 2021

MCM9 is associated with germline predisposition to early-onset cancer-clinical evidence.

NPJ Genom Med 2021 Sep 23;6(1):78. Epub 2021 Sep 23.

Genetic Institute, HaEmek Medical Center, Afula, Israel.

Mutated MCM9 has been associated with primary ovarian insufficiency. Although MCM9 plays a role in genome maintenance and has been reported as a candidate gene in a few patients with inherited colorectal cancer (CRC), it has not been clearly established as a cancer predisposition gene. We re-evaluated family members with MCM9-associated fertility problems. Read More

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September 2021

Assessment of mitotic activity in breast cancer: revisited in the digital pathology era.

J Clin Pathol 2021 Sep 23. Epub 2021 Sep 23.

Division of Cancer and Stem Cell, University of Nottingham, Nottingham, UK

The assessment of cell proliferation is a key morphological feature for diagnosing various pathological lesions and predicting their clinical behaviour. Visual assessment of mitotic figures in routine histological sections remains the gold-standard method to evaluate the proliferative activity and grading of cancer. Despite the apparent simplicity of such a well-established method, visual assessment of mitotic figures in breast cancer (BC) remains a challenging task with low concordance among pathologists which can lead to under or overestimation of tumour grade and hence affects management. Read More

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September 2021

Coronary Microvascular Dysfunction Across the Spectrum of Cardiovascular Diseases: JACC State-of-the-Art Review.

J Am Coll Cardiol 2021 Sep;78(13):1352-1371

Department of Cardiovascular and Pulmonary Sciences, Catholic University of the Sacred Heart, Rome, Italy; Department of Cardiovascular Medicine, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.

Coronary microvascular dysfunction (CMD) encompasses several pathogenetic mechanisms involving coronary microcirculation and plays a major role in determining myocardial ischemia in patients with angina without obstructive coronary artery disease, as well as in several other conditions, including obstructive coronary artery disease, nonischemic cardiomyopathies, takotsubo syndrome, and heart failure, especially the phenotype associated with preserved ejection fraction. Unfortunately, despite the identified pathophysiological and prognostic role of CMD in several conditions, to date, there is no specific treatment for CMD. Due to the emerging role of CMD as common denominator in different clinical phenotypes, additional research in this area is warranted to provide personalized treatments in this "garden variety" of patients. Read More

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September 2021