51,038 results match your criteria diagnose anal


Diet and nutrition in patients who have received pelvic radiotherapy: A mixed-methods study to explore dietary habits, nutritional awareness, and experiences of nutritional care.

Nutrition 2021 Apr 30;89:111309. Epub 2021 Apr 30.

Oxford School of Nursing and Midwifery, Oxford Institute of Nursing, Midwifery and Allied Health Research, Oxford, UK.

Objectives: Individuals who have survived cancer may benefit from dietary modifications to improve quality of life and future health outcomes. The aim of this study was to explore dietary habits and experiences of nutritional support in patients with a cancer diagnosis who have received radiotherapy to the pelvic area.

Methods: A mixed-methods approach was used. Read More

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Wireless Battery-Free Generation of Electric Fields on One-Dimensional Asymmetric Au/ZnO Nanorods for Enhanced Raman Sensing.

Anal Chem 2021 Jun 24. Epub 2021 Jun 24.

College of Sciences, Northeastern University, Shenyang 110004, China.

Wearable electronics have great potential in enhancing health monitoring, disease diagnosis, and environmental pollution tracking. Development of wearable surface-enhanced Raman spectroscopy (SERS) substrates with target sampling and sensitive sensing functions is a promising way to obtain physical and chemical information. This study describes a facile and effective approach for constructing an electrically modulated SERS (E-SERS) substrate as a wearable and wireless battery-free substrate with improved sensitivity. Read More

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Utility of process capability indices in assessment of quality control processes at a clinical laboratory chain.

J Clin Lab Anal 2021 Jun 24:e23878. Epub 2021 Jun 24.

Laboratory Diagnosis Department, Guangzhou KingMed Center for Clinical Laboratory, Guangzhou, China.

Background: To evaluate the utility of the process capability indices C and C for assessing the quality control processes at chain laboratory facilities.

Methods: In April 2020, the minimum C and C values for 33 assays of a laboratory chain with 19 facilities were collected for further analysis and a total of 627 datasets (C and C ) were compared. In addition, standard values for C and C , defined as the lowest of the top 20%, were obtained for comparison and the indices were used to determine whether precision or trueness improvements were required for the corresponding assay. Read More

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The role of circRNA derived from RUNX2 in the serum of osteoarthritis and its clinical value.

J Clin Lab Anal 2021 Jun 24:e23858. Epub 2021 Jun 24.

Institute of Chemical Engineering, Huaqiao University, Xiamen, China.

Background: Circular RNA (circRNA) has been shown to affect the pathological process of osteoarthritis (OA) and is expected to become a potential marker for disease diagnosis. This study aimed to investigate the association between circRNA derived from the gene of runt-related transcription factor 2 (RUNX2) and OA risk.

Methods: The expression profile of RUNX2-derived circRNAs in serum of OA patients was detected. Read More

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Down-regulation of hsa_circ_0006470 predicts tumor invasion: A new biomarker of gastric cancer.

J Clin Lab Anal 2021 Jun 24:e23879. Epub 2021 Jun 24.

Zhejiang Key Laboratory of Pathophysiology, Department of Biochemistry and Molecular Biology, Medical School of Ningbo University, Ningbo, Zhejiang, China.

Background: Gastric cancer (GC) is a common cancer. Circular RNAs (circRNAs) regulate the pathogenesis of GC. This study aims to explore its potential as a GC biomarker. Read More

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A novel time-resolved fluoroimmunoassay based on magnetic microspheres method for detecting antibodies against the phospholipase A2 receptor.

Anal Methods 2021 Jun 24. Epub 2021 Jun 24.

Immunoassay Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310016, China.

Background: the level of serum antibodies against the M-type phospholipase A2 receptor (anti-PLA2R-IgG) is closely related to the disease activity of idiopathic membranous nephropathy (IMN). Therefore, the establishment of a sensitive and rapid method for detecting anti-PLA2R-IgG will be beneficial for the differential diagnosis of IMN.

Methods: magnetic microspheres coupled with the PLA2R antigen were used to capture anti-PLA2R-IgG in serum samples, and europium-labeled goat anti-human IgG antibodies were used for tracking. Read More

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Point-of-Care Test Paper for Exhaled Breath Aldehyde Analysis via Mass Spectrometry.

Anal Chem 2021 Jun 24. Epub 2021 Jun 24.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Volatile organic compounds (VOCs) from exhaled breath (EB) are considered to be promising biomarkers for lung diseases. A convenient and sensitive point-of-care (POC) testing method for EB VOCs is essential. Here, we developed a POC test paper for the analysis of EB aldehydes, which are potential biomarkers for lung cancer. Read More

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Dual-Targeting Nanoprobe for Early Diagnosis of Pheochromocytoma Though Coinstantaneous Identification of Circulating Tumor Cells.

Anal Chem 2021 Jun 23. Epub 2021 Jun 23.

Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing, 211198, People's Republic of China.

Circulating tumor cells (CTCs) play a pivotal role in the early diagnosis of pheochromocytoma (PCC). Herein, we fabricated a new dual-targeting nanoprobe for coinstantaneous identification of rare PCC-CTCs from peripheral blood via targeting the norepinephrine transporter (NET) and somatostatin receptor SSTR2 overexpressed on the surface of PCC cells. Meta-iodobenzylguanidine (MIBG) functionalized magnetic FeO and octreotide (DOTA) decorated signal amplification [email protected] nanosphere were used to capture and detect PCC-CTCs by binding to NET and SSTR2. Read More

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Furin Enzyme and pH Synergistically Triggered Aggregation of Gold Nanoparticles for Activated Photoacoustic Imaging and Photothermal Therapy of Tumors.

Anal Chem 2021 Jun 23. Epub 2021 Jun 23.

State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X) and Collaborative Innovation Centre of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, 199 Renai Road, Suzhou 215123, China.

Specific and effective accumulation of nanoparticles within tumors is highly crucial for precise cancer diagnosis and treatment. Therefore, spatiotemporally manipulating the aggregation of small gold nanoparticles (AuNPs) in a tumor microenvironment is of great significance for enhancing the diagnostic and therapeutic efficacy of tumors. Herein, we reported a novel furin enzyme/acidic pH synergistically triggered small AuNP aggregation strategy for activating the photoacoustic (PA) imaging and photothermal (PTT) functions of AuNPs in vivo. Read More

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The Impact of COVID-19 pandemic on a tertiary referral proctology center: no one should be left behind.

Minerva Surg 2021 Jun 23. Epub 2021 Jun 23.

SOSD Proctologia USL Toscana Centro, Florence, Italy.

Background: Coronavirus disease 2019 (COVID-19) has disrupted healthcare delivery. We aimed to describe a novel strategy to mitigate the impact of COVID-19 pandemic on a tertiary referral proctology center during the first wave of infection in Italy.

Methods: All patients booked appointments at the Proctology Unit between March 9th and May 4th 2020 were identified. Read More

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Extramammary Paget disease: five perianal case report and treatment options.

J Surg Case Rep 2021 Jun 17;2021(6):rjab262. Epub 2021 Jun 17.

Anorectal Surgery Center, First Hospital Affiliated Anhui University of Chinese Medicine, Hefei, China.

We present five cases of perianal Paget disease (PPD). Two cases underwent a wide local excision (WLE) of PPD plus skin flap transfer surgery with good curative effect. One case of PPD complicated with mucinous adenocarcinoma underwent a laparoscopic abdominoperineal resection (Miles), which may be the extremes of clinical treatment of the disease. Read More

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Outcomes of Patients With Metastatic Anal Cancer According to HIV Infection: A Multicenter Study by the Latin American Gastrointestinal Oncology Group (SLAGO).

Clin Colorectal Cancer 2021 May 24. Epub 2021 May 24.

A.C. Camargo Cancer Center, Fundação Antonio Prudente, Sao Paulo, Brazil. Electronic address:

Background: HIV-positive patients are underrepresented in clinical trials of metastatic squamous cell carcinoma of the anal canal (mSCCA). We aimed to compare the clinical outcomes of mSCCA patients according to HIV infection.

Methods: This was a retrospective multicenter cohort study of consecutive patients with mSCCA. Read More

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Colonic Disease Recurrence following Proctectomy with End Colostomy for Anorectal Crohn's Disease.

Colorectal Dis 2021 Jun 22. Epub 2021 Jun 22.

Department of Surgery, Mount Sinai Hospital, University of Toronto, Canada.

Aim: In patients with anorectal Crohn's disease, it remains uncertain whether a total proctocolectomy with end ileostomy or proctectomy with end colostomy should be recommended due to the unknown rate of disease recurrence in the remaining colon.

Methods: A retrospective review of all patients with a known diagnosis of Crohn's disease who underwent a proctectomy with end colostomy for distal Crohn's disease between January 1, 2010 and January 1, 2019 at two IBD referral centers was conducted. Data collected included patient demographics, surgical variables at the time of proctectomy, and postoperative clinical, endoscopic and surgical recurrence rates. Read More

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Disease-image-specific Learning for Diagnosis-oriented Neuroimage Synthesis with Incomplete Multi-Modality Data.

IEEE Trans Pattern Anal Mach Intell 2021 Jun 22;PP. Epub 2021 Jun 22.

Incomplete data problem is commonly existing in disease diagnosis with multi-modality neuroimages, to track which, some methods have been proposed to utilize all available subjects by imputing missing neuroimages. However, these methods usually treat image synthesis and disease diagnosis as two standalone tasks, thus ignoring the specificity conveyed in different modalities, i.e. Read More

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Hairpin-Spherical Nucleic Acids for Diagnosing COVID-19: a Simple Method to Generalize the Conventional PCR for Molecular Assays.

Anal Chem 2021 Jun 22. Epub 2021 Jun 22.

Department of Molecular Diagnosis, Farzan Molecular and Pathobiology Laboratory, Hamedan 6515638377, Iran.

The COVID-19 pandemic revealed during the first global wave of this infectious disease that mass diagnostic testing was necessary to more rapidly detect infection in patients and control the pandemic. Therefore, extra research efforts to develop reliable and more accessible techniques for disease diagnosis are of supreme importance. Here, a target-responsive assembly of gold nanoparticle-core hairpin-spherical nucleic acids (AuNP-core H-SNAs) was implemented to modify the conventional polymerase chain reaction (PCR) assay for the "naked-eye" colorimetric detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. Read More

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Investigation of the mechanical effects of targeted drugs on cancerous cells based on atomic force microscopy.

Anal Methods 2021 Jun 22. Epub 2021 Jun 22.

School of Engineering, University of Warwick, Coventry, CV4 7AL, UK.

Cancer is currently drawing more and more attention as the leading factor in death worldwide. However, little research has been directed towards investigating the micro/nanoscale mechanical properties of cancer cells treated by targeted drugs to evaluate the model systems of targeted drugs using atomic force microscopy (AFM) nano-indentation, especially in light of the multiple drugs targeting various cancerous cells. This paper aims to compare the mechanical effects of sorafenib tosylate and osimertinib mesylate on hepatoma carcinoma cells and lung cancerous cells using atomic force microscopy from the perspective of a model system based on nano-indentation at the micro/nanoscale, which has rarely been investigated. Read More

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On-Site Detection of SARS-CoV-2 Antigen by Deep Learning-Based Surface-Enhanced Raman Spectroscopy and Its Biochemical Foundations.

Anal Chem 2021 Jun 22. Epub 2021 Jun 22.

Laser Fusion Research Center, China Academy of Engineering Physics, 621900 Mianyang, China.

A rapid, on-site, and accurate SARS-CoV-2 detection method is crucial for the prevention and control of the COVID-19 epidemic. However, such an ideal screening technology has not yet been developed for the diagnosis of SARS-CoV-2. Here, we have developed a deep learning-based surface-enhanced Raman spectroscopy technique for the sensitive, rapid, and on-site detection of the SARS-CoV-2 antigen in the throat swabs or sputum from 30 confirmed COVID-19 patients. Read More

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Hydrostatic anorectal injury caused by fall from jet-ski.

BMJ Case Rep 2021 Jun 21;14(6). Epub 2021 Jun 21.

Bar-Ilan University Faculty of Medicine, Tzfat, Israel

A 14-year-old girl, a rear seat passenger on a jet-ski not wearing suitable protective gear, was 'jumping' waves at a low velocity when she was ejected backwards off the vehicle, suffering a complex and unusual hydrostatic perineal injury as a result of the high-pressure water stream propelling the jet-ski. She presented to the emergency room with rectal bleeding and perineal and abdominal pain. Initial investigations revealed signs of anorectal injury and both intraperitoneal and extraperitoneal free air and fluid, suggesting a possible rectal perforation. Read More

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Response to Pembrolizumab in Advanced Anal Squamous Cell Carcinoma With High TMB and PD-L1 and PD-L2 Amplification.

Clin Colorectal Cancer 2021 May 24. Epub 2021 May 24.

Centro de Oncologia e Hematologia Einstein Familia Dayan-Daycoval. Electronic address:

We report the case of a 44-year-old female with a prior diagnosis of Sjögren's syndrome who was treated for metastatic anal squamous cell carcinoma with second-line pembrolizumab and has achieved a sustained partial response after a follow-up of 13 months. Comprehensive genomic profiling was remarkable for PD-L1 and PD-L2 amplification and a high tumor mutational burden (19 mutations per megabase). To the best of our knowledge, we present the first report to correlate PD-L1 and PD-L2 amplification with good outcomes of immune checkpoint inhibition in metastatic anal squamous cell carcinoma. Read More

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NS1 glycoprotein detection in serum and urine as an electrochemical screening immunosensor for dengue and Zika virus.

Anal Bioanal Chem 2021 Jun 21. Epub 2021 Jun 21.

Biomedical Engineering Laboratory, Federal University of Pernambuco, Recife, PE, 50670-901, Brazil.

The incidence of infection by the dengue virus (DENV) has grown dramatically, reaching 128 countries in tropical and subtropical regions worldwide, with a pattern of hyper-endemicity. DENV is a mosquito-borne disease having four serotypes, one or two circulating in epidemic outbreaks. The diagnosis of DENV is challenging mainly due to the circulation of new viruses with remarkable similarities, such as Zika (ZIKV) that may cause fetal microcephaly. Read More

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Detection of Enterovirus, Herpes Simplex, Varicella Zoster, Epstein-Barr and Cytomegalovirus in cerebrospinal fluid in meningitis patients in Iran.

J Clin Lab Anal 2021 Jun 21:e23836. Epub 2021 Jun 21.

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Despite medical advances, central nervous system (CNS) diseases put a pressure on the health care system. A number of risk factors, especially infectious agents can accelerate the progression of meningitis. As viruses probably account for most cases of meningitis, the diagnosis of them can reduce antibiotic prescriptions. Read More

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Electrochemical Deposition of Cu Metal-Organic Framework Films for the Dual Analysis of Pathogens.

Anal Chem 2021 Jun 21. Epub 2021 Jun 21.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China.

Metal-organic framework (MOF) thin films with flexible nature and prominent qualities have opened doors to new technological applications in different fields. Herein, we propose an electrochemical biosensor for the dual detection of Staphylococcus aureus based on the electrodeposition of Cu metal-organic framework (Cu-MOF) thin films. The promising sensing layer with features of good electronic conductivity and enhanced electron-transfer property can not only identify through specific micrococcal nucleases in the supernatant but also detect the pathogen directly aptamer recognition. Read More

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Combined detection of procalcitonin, heparin-binding protein, and interleukin-6 is a promising assay to diagnose and predict acute pancreatitis.

J Clin Lab Anal 2021 Jun 21:e23869. Epub 2021 Jun 21.

Department of Emergency Medicine, Laboratory of Emergency Medicine, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Acute pancreatitis (AP), one of the most common clinical emergencies, is characterized by variable clinical features and inadequate diagnostic methods. At present, the commonly used indicators do not have high specificity and do not necessarily reflect disease severity. We therefore aimed to investigate diagnostic and prognostic value of plasma procalcitonin, heparin-binding protein, and interleukin-6 for acute pancreatitis by separate detection and joint detection. Read More

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[Efficacy of transanal hand-sewn reinforcement in low rectal stapled anastomosis in preventing anastomotic leak after transanal total mesorectal excision].

Zhonghua Wei Chang Wai Ke Za Zhi 2021 Jun;24(6):530-535

Department of Colorectal Surgery, Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China.

To explore the efficacy and feasibility of transanal hand-sewn reinforcement of low stapled anastomosis in preventing anastomotic leak after transanal total mesorectal excision (taTME). A descriptive cohort study was conducted. Clinical data of 51 patients with rectal cancer who underwent taTME with transanal hand-sewn reinforcement of low stapled anastomosis at Department of Colorectal Surgery, the Sixth Affiliated Hospital of Sun Yat-sen University from January 2019 to December 2020 were retrospectively collected. Read More

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[Clinical features and prognosis of anastomotic leak after anterior resection for rectal cancer following neoadjuvant chemoradiotherapy].

Zhonghua Wei Chang Wai Ke Za Zhi 2021 Jun;24(6):513-522

Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Sun Yat-sen University, Guangzhou 510655, China.

To explore clinical features and prognosis of anastomotic leak (AL) after anterior resection following neoadjuvant chemoradiotherapy for rectal cancer patients. A retrospective cohort study was performed. Data were retrieved from colorectal cancer database of the Sixth Affiliated Hospital, Sun Yat-sen University. Read More

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[Incidence and risk factors of anastomotic leak after transanal total mesorectal excision in China: a retrospective analysis based on national database].

Zhonghua Wei Chang Wai Ke Za Zhi 2021 Jun;24(6):505-512

Department of General Surgery, Beijing Friendship Hospital, Capital Medical University; Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center for Digestive Diseases, Beijing 100050, China.

Transanal total mesorectal excision (taTME) was a very hot topic in the first few years since its appearance, but now more introspections and controversies on this procedure have emerged. One of the reasons why the Norwegian Ministry of Health stopped taTME was the high incidence of postoperative anastomotic leak. In current study, the incidence and risk factors of anastomotic leak after taTME were analyzed based on the data registered in the Chinese taTME Registry Collaborative (CTRC). Read More

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[Research progress on early diagnosis and prevention of anastomotic leak after rectal cancer surgery].

Zhonghua Wei Chang Wai Ke Za Zhi 2021 Jun;24(6):480-486

Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, & National Clinical Research Center for Digestive Diseases, Beijing 100050, China.

Anastomotic leak is a common and serious complication after anterior rectal resection. Despite the continuous advancement of anastomotic instruments and surgical techniques, the incidence of anastomotic leak has not decreased significantly compared with the past. As more studies on the early diagnosis of anastomotic leak are published, postoperative risk factors of anastomotic leak, such as fever, time to first bowel movement, CT, C-reactive protein (CRP) and procalcitonin (PCT), matrix metalloproteinase-9, and other cytokines and biomarkers (IL-6, TNF-α, lactate, pH, urinary neopterin/creatinine ratio), provide a reference for surgeons to assess the risk and increase the possibility of early diagnosis of anastomotic leak. Read More

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Dynamic Esophageal Patency Assessment: An effective method for prenatally diagnosing esophageal atresia.

Am J Obstet Gynecol 2021 Jun 16. Epub 2021 Jun 16.

Diagnostic Ultrasound Unit of the Institute of Obstetrical and Gynecological Imaging, Department of Obstetrics and Gynecology, Sheba Medical Center, Tel-Hashomer, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel. Electronic address:

Objective: Esophageal atresia is a major anomaly with a low prenatal detection rate. We propose a sonographic method termed Dynamic Esophageal Patency Assessment (DEPA). The aim of the study was to assess the feasibility and performance of this method in a high-risk population. Read More

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Nanozyme Sensor Array Plus Solvent-Mediated Signal Amplification Strategy for Ultrasensitive Ratiometric Fluorescence Detection of Exosomal Proteins and Cancer Identification.

Anal Chem 2021 Jun 18. Epub 2021 Jun 18.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.

Tumor exosomes with molecular marker-proteins inherited from their parent cells have emerged as a promising liquid biopsy biomarker for cancer diagnosis. However, facile, robust, and sensitive detection of exosomal proteins remains challenging. Therefore, a nanozyme sensor array is constructed by using aptamer-modified CN nanosheets (Apt/CN NSs) together with a solvent-mediated signal amplification strategy for ratiometric fluorescence detection of exosomal proteins. Read More

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The application of aptamer Apt-236 targeting PvpA protein in the detection of antibodies against .

Anal Methods 2021 Jun 18. Epub 2021 Jun 18.

Key Laboratory of Animal Epidemiology and Zoonosis, College of Veterinary Medicine, China Agricultural University, Beijing 100083, China.

Mycoplasma gallisepticum (M. gallisepticum) is the primary agent of chronic respiratory disease causing important economic losses in the poultry industry. Compared to antibodies, aptamers used to diagnose M. Read More

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