17 results match your criteria dg2 dg3

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Assessment of dysplasia in bone marrow smear with convolutional neural network.

Sci Rep 2020 09 7;10(1):14734. Epub 2020 Sep 7.

Department of Hemato-Oncology, International Medical Center, Saitama Medical University, Saitama, Japan.

In this study, we developed the world's first artificial intelligence (AI) system that assesses the dysplasia of blood cells on bone marrow smears and presents the result of AI prediction for one of the most representative dysplasia-decreased granules (DG). We photographed field images from the bone marrow smears from patients with myelodysplastic syndrome (MDS) or non-MDS diseases and cropped each cell using an originally developed cell detector. Two morphologists labelled each cell. Read More

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September 2020

Performance evaluation of anti-fixed Leishmania infantum promastigotes immunoglobulin G (IgG) detected by flow cytometry as a diagnostic tool for visceral Leishmaniasis.

J Immunol Methods 2019 06 25;469:18-25. Epub 2019 Feb 25.

Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Instituto Aggeu Magalhães, Recife, Pernambuco, Brazil.

Visceral Leishmaniasis (VL) is a severe disease, caused by the protozoans Leishmania infantum and L. donovani that is widely diagnosed using serological tools. These, however, have limitations in performance that limit their use for the correct identification of the cases. Read More

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The predictive power of the elimination of dioxin-like pollutants from pigs: an in vivo study.

Environ Int 2012 Jan 22;38(1):73-8. Epub 2011 Sep 22.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, PR China.

Pigs accidentally given feed contaminated by dioxin-like pollutants are a serious public health issue. We have examined whether pigs with limited exposure during early periods of fattening would be categorized as non-compliant with the EU limit at slaughtering when growth-dilution, excretion and metabolism effects are considered. Sixteen female and sixteen castrated male weaned pigs were divided into four groups (e. Read More

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January 2012

Ruminal phosphorus disappearance from corn and soybean feedstuffs.

J Dairy Sci 2008 Oct;91(10):3938-46

Dairy Science Department, South Dakota State University, Brookings 57007, USA.

Byproducts of corn and soybeans have high phosphorus (P) content, but little is known about their P disappearance in the rumen of lactating dairy cows. In situ disappearance of P from corn and soybean feed-stuffs was determined in 2 experiments. In the first experiment, 3 ruminally cannulated lactating dairy cows were used to estimate in situ P disappearance of 9 feed ingredients that included 3 sources of dried distillers grains with solubles (DDG; DGa, DGb, DGc), corn, corn germ, solvent-extracted soybean meal, (44% CP; SBM), expeller soybean meal (SoyPlus; SP), extruded soybeans (ES), and soyhulls (SH). Read More

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October 2008

Linear-dendritic supramolecular complexes as nanoscale reaction vessels for "green" chemistry. Diels-Alder reactions between fullerene C60 and polycyclic aromatic hydrocarbons in aqueous medium.

Langmuir 2008 Oct 10;24(20):11431-41. Epub 2008 Sep 10.

Department of Chemistry, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210, USA.

This study describes the first Diels-Alder (DA) reaction performed in aqueous medium with highly hydrophobic compounds-fullerene (C 60) as the dienophile and anthracene (An) or tetracene (Tet) as the dienes, respectively. The reactions are performed in nanocontainers, constructed by self-assembly of linear-dendritic amphiphilic copolymers with poly(ethylene glycol), PEG or poly(ethylene oxide), PEO as the hydrophilic blocks and poly(benzyl ether) monodendrons as the hydrophobic fragments: G3PEO13k, dG3 and dG2. Comparative studies under identical conditions are carried out with an amphiphilic linear-linear copolymer, poly(styrene)1800- block-PEO2100, PSt-PEO, and the nonionic surfactant Igepal CO-720, IP720. Read More

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October 2008

Ruminal and intestinal degradability of distillers grains plus solubles varies by source.

J Dairy Sci 2007 Jun;90(6):2909-18

Dairy Science Department, South Dakota State University, Brookings 57007, USA.

Currently in the dairy industry, there is a concern about the variability in the nutrient content among sources of distillers grains plus solubles (DG), but little research has evaluated the variability in metabolizable AA among sources. The ruminal degradability of crude protein (CP) in soybean meal (SBM), dried DG from 5 sources (DG1, DG2, DG3, DG4, and DG5), and 1 source of wet DG (WDG) were determined using 2 lactating ruminally cannulated Holstein cows. Feeds were incubated in the rumen for 3, 6, 12, 18, 24, and 36 h. Read More

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Application of identified QTL-marker associations in rice quality improvement through a design-breeding approach.

Theor Appl Genet 2007 Jun 4;115(1):87-100. Epub 2007 May 4.

Institute of Crop Science and The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, China.

A permanent mapping population of rice consisting of 65 non-idealized chromosome segment substitution lines (denoted as CSSL1 to CSSL65) and 82 donor parent chromosome segments (denoted as M1 to M82) was used to identify QTL with additive effects for two rice quality traits, area of chalky endosperm (ACE) and amylose content (AC), by a likelihood ratio test based on stepwise regression. Subsequently, the genetics and breeding simulation tool QuLine was employed to demonstrate the application of the identified QTL in rice quality improvement. When a LOD threshold of 2. Read More

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Redesign of artificial globins: effects of residue replacements at hydrophobic sites on the structural properties.

Biochemistry 2000 May;39(19):5683-90

The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Artificial sequences of the 153 amino acids have been designed to fit the main-chain framework of the sperm whale myoglobin (Mb) structure based on a knowledge-based 3D-1D compatibility method. The previously designed artificial globin (DG1) folded into a monomeric, compact, highly helical and globular form with overall dimensions similar to those of the target structure, but it lacked structural uniqueness at the side-chain level [Isogai, Y., Ota, M. Read More

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Metabolism of digoxin and digoxigenin digitoxosides in rat liver microsomes: involvement of cytochrome P4503A.

Xenobiotica 1999 Feb;29(2):171-85

Department of Biopharmaceutical Sciences, School of Pharmacy, University of California, San Francisco 94143-0446, USA.

1. The sequential metabolism of digoxin (Dg3) to digoxigenin bis-digitoxoside (Dg2), digoxigenin mono-digitoxoside (Dg1) and digoxigenin (Dg0) was investigated in rat liver microsomes. 2. Read More

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February 1999

The validity of two quality assessment instruments: Monitor and Senior Monitor.

Int J Nurs Stud 1996 Dec;33(6):660-8

Department of Nursing Studies, King's College London, U.K.

This paper is concerned with validity of Monitor and Senior Monitor. The study carried out, which was funded by the Department of Health, attempted to establish the validity of the instruments through a multiple triangulation research design. Monitor and Senior Monitor were compared to another instrument, Qualpacs, and also to other methods that focused on quality of nursing care--observation of patients' activities and interactions with nurses, and interviews with patients and nurses on their perceptions of quality. Read More

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December 1996

Cloning and sequence analysis of desmosomal glycoproteins 2 and 3 (desmocollins): cadherin-like desmosomal adhesion molecules with heterogeneous cytoplasmic domains.

J Cell Biol 1991 Apr;113(2):381-91

Cancer Research Campaign, University of Southampton, Southampton General Hospital, United Kingdom.

Desmosomal glycoproteins 2 and 3 (dg2 and 3) or desmocollins have been implicated in desmosome adhesion. We have obtained a 5.0-kb-long clone for dg3 from a bovine nasal epidermal lambda gt11 cDNA library. Read More

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An immunohistological study of desmosomes in Darier's disease and Hailey-Hailey disease.

Br J Dermatol 1991 Mar;124(3):242-51

Department of Dermatology, Slade Hospital, Oxford, U.K.

The immunocytochemical distribution of desmosomal components was determined in involved skin from eight patients with Darier's disease, five patients with Hailey-Hailey disease and two patients with transient acantholytic dermatosis as well as skin from four normal controls. Sections were stained using monoclonal antibodies to the desmosomal proteins dp1 and dp2 (desmoplakins) and the desmosomal glycoproteins dg1 (desmoglein), and dg2 and dg3 (desmocollins). There was normal expression of desmosomal proteins and glycoproteins at the periphery of the keratinocytes in the perilesional skin in Darier's disease, in Hailey-Hailey disease and in transient acantholytic dermatosis. Read More

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Metabolism of digoxin, digoxigenin digitoxosides and digoxigenin in human hepatocytes and liver microsomes.

Fundam Clin Pharmacol 1991 ;5(7):567-82

INSERM U 278, Faculté de Pharmacie, Marseille, France.

In vitro metabolism of digoxin and its cleavage-related compounds was investigated using hepatocytes in primary culture and microsomal fractions both isolated from human livers. On these models, digoxin (DG3) and digoxigenin bisdigitoxoside (DG2) were not shown to be significantly metabolized in vitro in man. Therefore, it appeared that the stepwise cleavage of DG3 and DG2 sugars was not cytochrome P450 dependent. Read More

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Desmosomal glycoproteins 2 and 3 (desmocollins) show N-terminal similarity to calcium-dependent cell-cell adhesion molecules.

J Cell Sci 1990 Oct;97 ( Pt 2):239-46

Cancer Research Campaign Medical Oncology Unit, University of Southampton, Southampton General Hospital, UK.

The N-terminal sequence of a mixture of desmosomal glycoproteins 2 and 3 (dg2/3, desmocollins) from bovine nasal epidermis, prepared by electro-elution from polyacrylamide gels, was determined by solid-phase Edman degradation. A sequence of 23 amino acids was obtained. This showed 43% identity with that of the N terminus of the calcium-dependent cell adhesion molecule, N-cadherin. Read More

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October 1990

Size heterogeneity, phosphorylation and transmembrane organisation of desmosomal glycoproteins 2 and 3 (desmocollins) in MDCK cells.

J Cell Sci 1990 Jun;96 ( Pt 2):239-48

Cancer Research Campaign Medical Oncology Unit, University of Southampton, UK.

Metabolic labelling with [35S]methionine and immunoprecipitation with specific antibodies to bovine desmosomal glycoproteins 2 and 3 (dg2 and dg3: desmocollins) reveals a triplet of polypeptides of Mr 115,000, 107,000 and 104,000 in MDCK cells. Tunicamycin treatment shows that this heterogeneity does not arise through differential N-linked glycosylation. Under conditions in which cells are actively forming desmosomes, the largest polypeptide, dg2, becomes phosphorylated on serine, but the two smaller polypeptides, dg3a and 3b, do not. Read More

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Establishment of cell lines from serial samples from two patients with lung tumours before and after chemotherapy.

Cytotechnology 1989 Feb;2(1):39-47

Laboratorio di Chemioterapia Sperimentale Preclinica, Istituto Regina Elena, Roma, Italy.

Six cell lines were derived from pleural effusions of two lung cancer patients and established in vitro in our laboratory. Cell line AE1 was obtained from a small cell lung cancer (SCLC) before the patient had received any chemotherapy; the other lines (AE2 and AE3) were established from tumour recurrences in the same patient after therapy. Cell lines DG1 and DG2 were derived from specimens of an untreated non-small cell lung cancer (NSCLC), while cell line DG3 originated from pleural effusions recurring in the same patient after therapy. Read More

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February 1989

Localisation of the protein and glycoprotein components of bovine nasal epithelial desmosomes by immunoelectron microscopy.

EMBO J 1987 Apr;6(4):885-9

Desmosomal proteins (dp1-4) and glycoproteins (dg1-3) have been localised within desmosomes of bovine nasal epithelium by immunogold labelling of ultrathin frozen sections. Beginning in the extracellular space and proceeding through the plaque to the tonofilaments, the following localisations were found. Labelling for the 130,000 and 115,000 Mr glycoproteins (dg2 and dg3) was predominantly in the extracellular space, a location consistent with their proposed adhesive function. Read More

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