35 results match your criteria dg1 dg2

Assessment of dysplasia in bone marrow smear with convolutional neural network.

Sci Rep 2020 09 7;10(1):14734. Epub 2020 Sep 7.

Department of Hemato-Oncology, International Medical Center, Saitama Medical University, Saitama, Japan.

In this study, we developed the world's first artificial intelligence (AI) system that assesses the dysplasia of blood cells on bone marrow smears and presents the result of AI prediction for one of the most representative dysplasia-decreased granules (DG). We photographed field images from the bone marrow smears from patients with myelodysplastic syndrome (MDS) or non-MDS diseases and cropped each cell using an originally developed cell detector. Two morphologists labelled each cell. Read More

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September 2020

Protective role of diosgenin against hyperglycaemia-mediated cerebral ischemic brain injury in zebrafish model of type II diabetes mellitus.

Heliyon 2020 Jan 31;6(1):e03296. Epub 2020 Jan 31.

Centre for Medical Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, 201303, India.

Impairment in glucose regulation is an indicatory effect capable of mediating multiple dysfunction such as cerebrovascular disorder with ischemia and brain damage inclusive. This study aims at investigating the glucose-lowering and neuroprotective capability of Diosgenin (DG) towards hyperglycemia-induced cerebral injury in a developed type 2 diabetes mellitus (T2DM) Zebrafish (ZF) model. T2DM was developed in ZF with 20 mg/kg body weight () multiple-low dose (MLD) Streptozotocin (STZ) for 28 days. Read More

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January 2020

Large inter-individual variability in pharmacokinetics of dexmedetomidine and its two major N-glucuronides in adult intensive care unit patients.

J Pharm Biomed Anal 2019 Oct 16;175:112777. Epub 2019 Jul 16.

School of Pharmacy, The Chinese University of Hong Kong, Hong Kong. Electronic address:

Dexmedetomidine (DMTD), an α-adrenoceptor agonist, is commonly used for sedation and analgesia in intensive care unit (ICU) patients. The primary plasma metabolites of DMTD are its direct N-glucuronides, namely N-glucuronide of dexmedetomidine (DG1) and N-glucuronide of dexmedetomidine (DG2), accounting for 41% of DMTD metabolism in healthy volunteers. Since variations on the extent of N-glucuronidation could be one of the key factors contributing to the high interpatient differences of DMTD pharmacokinetics in ICU patients and its subsequent sedative effect. Read More

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October 2019

Performance evaluation of anti-fixed Leishmania infantum promastigotes immunoglobulin G (IgG) detected by flow cytometry as a diagnostic tool for visceral Leishmaniasis.

J Immunol Methods 2019 06 25;469:18-25. Epub 2019 Feb 25.

Fundação Oswaldo Cruz (Fiocruz-Pernambuco), Instituto Aggeu Magalhães, Recife, Pernambuco, Brazil.

Visceral Leishmaniasis (VL) is a severe disease, caused by the protozoans Leishmania infantum and L. donovani that is widely diagnosed using serological tools. These, however, have limitations in performance that limit their use for the correct identification of the cases. Read More

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Kinesio Taping Application in Dysphonic Singers.

J Voice 2020 May 6;34(3):487.e11-487.e20. Epub 2018 Dec 6.

Ent Department, Yarmouk University, Irbid, Jordan.

Introduction: Kinesio Taping (KT) application in speech therapy has been studied in a few works about dysphonia, facial nerve palsy, sialorrhea, atypical deglutition, postsurgical recovery after thyroidectomy and laryngectomy. The aim of this study was the evaluation of the possible role of KT in supporting speech therapy in singers complaining of dysphonia using singing voice handicap index (SVHI), fundamental frequency (F0), shimmer, jitter and harmonic to noise ratio (mean H/N).

Materials And Methods: We enrolled consecutive singers and singing students complaining of dysphonia and voice problems. Read More

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Comparison between Two Linear Supervised Learning Machines' Methods with Principle Component Based Methods for the Spectrofluorimetric Determination of Agomelatine and Its Degradants.

J Fluoresc 2017 May 1;27(3):1149-1160. Epub 2017 Mar 1.

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.

Four accurate, sensitive and reliable stability indicating chemometric methods were developed for the quantitative determination of Agomelatine (AGM) whether in pure form or in pharmaceutical formulations. Two supervised learning machines' methods; linear artificial neural networks (PC-linANN) preceded by principle component analysis and linear support vector regression (linSVR), were compared with two principle component based methods; principle component regression (PCR) as well as partial least squares (PLS) for the spectrofluorimetric determination of AGM and its degradants. The results showed the benefits behind using linear learning machines' methods and the inherent merits of their algorithms in handling overlapped noisy spectral data especially during the challenging determination of AGM alkaline and acidic degradants (DG1 and DG2). Read More

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Kinesio Taping in Dysphonic Patients.

J Voice 2017 Sep 23;31(5):589-593. Epub 2017 Feb 23.

ENT Department, Policlinico S.M. alle Scotte, Siena, Italy. Electronic address:

Objective: Kinesio taping (KT) is a method of elastic bandaging that produces a biomechanical effect on the organism. This study aims to evaluate its use in dysphonic patients tratment, as integrated part of speech therapy.

Study Design: Randomized controlled MATERIALS AND METHODS: Fifteen dysphonic patients were given the option to complement the speech therapy treatment with KT (DG1). Read More

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September 2017

An assemblage of Frankia Cluster II strains from California contains the canonical nod genes and also the sulfotransferase gene nodH.

BMC Genomics 2016 10 12;17(1):796. Epub 2016 Oct 12.

Department of Ecology, Environment and Plant Sciences, Stockholm University, 106 91, Stockholm, Sweden.

Background: The ability to establish root nodule symbioses is restricted to four different plant orders. Soil actinobacteria of the genus Frankia can establish a symbiotic relationship with a diverse group of plants within eight different families from three different orders, the Cucurbitales, Fagales and Rosales. Phylogenetically, Frankia strains can be divided into four clusters, three of which (I, II, III) contain symbiotic strains. Read More

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October 2016

How to improve early case detection in low endemic areas with pockets of leprosy: a study of newly detected leprosy patients in Guizhou Province, People's Republic of China.

Lepr Rev 2016 Mar;87(1):23-31

Although leprosy in China is controlled at a low endemic level, the number of new cases in Guizhou province has shown no significant decrease over the past 20 years. Guizhou remains the province with the second highest prevalence in China. The authors conducted a study in which the characteristics of newly detected leprosy cases, found between 2008 and 2012 in Guizhou, were analysed. Read More

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Comparison of multiple quantitative MRI parameters for characterization of the goat cartilage in an ongoing osteoarthritis: dGEMRIC, T1ρ and sodium.

Z Med Phys 2016 Sep 24;26(3):270-82. Epub 2015 Dec 24.

MRB Research Center for Magnetic Resonance Bavaria, Am Hubland, 97074 Wuerzburg, Germany. Electronic address:

Rationale And Objectives: Osteoarthritis (OA) is a degenerative joint disease leading to cartilage deterioration by loss of matrix, fibrillation, formation of fissures, and ultimately complete loss of the cartilage surface. Here, three magnetic resonance imaging (MRI) techniques, dGEMRIC (delayed Gadolinium enhanced MRI of cartilage; dG1=T1,post; dG2=1/T1,post-1/T1,pre), T1ρ,and sodium MRI, are compared in a preclinical in vivo study to evaluate the differences in their potential for cartilage characterization and to establish an examination protocol for a following clinical study.

Materials And Methods: OA was induced in 12 caprine knees (6 control, 6 therapy). Read More

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September 2016

Mixed infection of Leishmania infantum and Leishmania braziliensis in rodents from endemic urban area of the New World.

BMC Vet Res 2015 Mar 20;11:71. Epub 2015 Mar 20.

Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brasil.

Background: In Brazil Leishmania braziliensis and L. infantum are the principal species responsible for cutaneous and visceral leishmaniases, respectively. Domestic dogs are the main reservoirs of visceral leishmaniasis, while rodents and marsupials are the main reservoirs for cutaneous leishmaniasis. Read More

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The predictive power of the elimination of dioxin-like pollutants from pigs: an in vivo study.

Environ Int 2012 Jan 22;38(1):73-8. Epub 2011 Sep 22.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, PR China.

Pigs accidentally given feed contaminated by dioxin-like pollutants are a serious public health issue. We have examined whether pigs with limited exposure during early periods of fattening would be categorized as non-compliant with the EU limit at slaughtering when growth-dilution, excretion and metabolism effects are considered. Sixteen female and sixteen castrated male weaned pigs were divided into four groups (e. Read More

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January 2012

Behavioral responses to hypoxia in Drosophila larvae are mediated by atypical soluble guanylyl cyclases.

Genetics 2010 Sep 30;186(1):183-96. Epub 2010 Jun 30.

Department of Integrative Biosciences, Oregon Health and Science University, Portland, Oregon 97239, USA.

The three Drosophila atypical soluble guanylyl cyclases, Gyc-89Da, Gyc-89Db, and Gyc-88E, have been proposed to act as oxygen detectors mediating behavioral responses to hypoxia. Drosophila larvae mutant in any of these subunits were defective in their hypoxia escape response-a rapid cessation of feeding and withdrawal from their food. This response required cGMP and the cyclic nucleotide-gated ion channel, cng, but did not appear to be dependent on either of the cGMP-dependent protein kinases, dg1 and dg2. Read More

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September 2010

Ruminal phosphorus disappearance from corn and soybean feedstuffs.

J Dairy Sci 2008 Oct;91(10):3938-46

Dairy Science Department, South Dakota State University, Brookings 57007, USA.

Byproducts of corn and soybeans have high phosphorus (P) content, but little is known about their P disappearance in the rumen of lactating dairy cows. In situ disappearance of P from corn and soybean feed-stuffs was determined in 2 experiments. In the first experiment, 3 ruminally cannulated lactating dairy cows were used to estimate in situ P disappearance of 9 feed ingredients that included 3 sources of dried distillers grains with solubles (DDG; DGa, DGb, DGc), corn, corn germ, solvent-extracted soybean meal, (44% CP; SBM), expeller soybean meal (SoyPlus; SP), extruded soybeans (ES), and soyhulls (SH). Read More

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October 2008

Regio- and stereospecific N-glucuronidation of medetomidine: the differences between UDP glucuronosyltransferase (UGT) 1A4 and UGT2B10 account for the complex kinetics of human liver microsomes.

Drug Metab Dispos 2008 Aug 12;36(8):1529-37. Epub 2008 May 12.

Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P.O. Box 56 (Viikinkaari 5), 00014 University of Helsinki, Finland.

Medetomidine is a chiral imidazole derivate whose dextroenantiomer is pharmacologically active. The major metabolic pathway of dexmedetomidine [(+)-4-(S)-[1-(2,3-dimethylphenyl)ethyl]-1H-imidazole] in humans is N-glucuronidation at the imidazolate nitrogens. We have purified the N3- and N1-glucuronides of dexmedetomidine, termed DG1 and DG2, respectively, according to their elution order in liquid chromatography and determined their structure by 1H nuclear magnetic resonance (NMR). Read More

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Targeting neuropilin 1 as an antitumor strategy in lung cancer.

Clin Cancer Res 2007 Aug;13(16):4759-68

National Taiwan University Center for Genomic Medicine, College of Medicine, National Taiwan University, Tainan, Taiwan.

Purpose: Neuropilin 1 (NRP1) is a mediator of lung branching and angiogenesis in embryonic development and angiogenesis in cancer. The role of NRP1 in cancer progression is not fully elucidated. We investigated the role of NRP1 in cancer invasion and tumor angiogenesis, its signaling pathways, prognostic significance, and therapeutic implications. Read More

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Ruminal and intestinal degradability of distillers grains plus solubles varies by source.

J Dairy Sci 2007 Jun;90(6):2909-18

Dairy Science Department, South Dakota State University, Brookings 57007, USA.

Currently in the dairy industry, there is a concern about the variability in the nutrient content among sources of distillers grains plus solubles (DG), but little research has evaluated the variability in metabolizable AA among sources. The ruminal degradability of crude protein (CP) in soybean meal (SBM), dried DG from 5 sources (DG1, DG2, DG3, DG4, and DG5), and 1 source of wet DG (WDG) were determined using 2 lactating ruminally cannulated Holstein cows. Feeds were incubated in the rumen for 3, 6, 12, 18, 24, and 36 h. Read More

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Application of identified QTL-marker associations in rice quality improvement through a design-breeding approach.

Theor Appl Genet 2007 Jun 4;115(1):87-100. Epub 2007 May 4.

Institute of Crop Science and The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, China.

A permanent mapping population of rice consisting of 65 non-idealized chromosome segment substitution lines (denoted as CSSL1 to CSSL65) and 82 donor parent chromosome segments (denoted as M1 to M82) was used to identify QTL with additive effects for two rice quality traits, area of chalky endosperm (ACE) and amylose content (AC), by a likelihood ratio test based on stepwise regression. Subsequently, the genetics and breeding simulation tool QuLine was employed to demonstrate the application of the identified QTL in rice quality improvement. When a LOD threshold of 2. Read More

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Lipid metabolism in trained rats: effect of guarana (Paullinia cupana Mart.) supplementation.

Clin Nutr 2005 Dec 22;24(6):1019-28. Epub 2005 Sep 22.

Department of Cell and Developmental Biology, Institute of Biomedical Sciences, Av. Prof. Lineu Prestes 1524, São Paulo, SP, CEP 05508-900, Brazil.

Background And Aims: Guarana is widely consumed by athletes, either in supplements or in soft drinks, under the belief that it presents ergogenic and "fat burning" effects. We examined the effect of guarana supplementation (14 days) upon aspects of lipid metabolism in sedentary (C) and trained rats (T).

Methods: To isolate the effect of caffeine from that of other components of guarana, we adopted two different doses of whole extract (G1-0. Read More

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December 2005

Analysis of Drosophila cGMP-dependent protein kinases and assessment of their in vivo roles by targeted expression in a renal transporting epithelium.

J Biol Chem 2004 Sep 24;279(38):40026-34. Epub 2004 Jun 24.

Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, Anderson College, Dumbarton Rd., Glasgow G11 6NU, United Kingdom.

cGMP-dependent protein kinase (cGK) forms encoded by the dg2 (for) gene are implicated in behavior and epithelial transport in Drosophila melanogaster. Here, we provide the first biochemical characterization and cellular localization of cGKs encoded by the major transcripts of dg2: dg2P1 and dg2P2. cGMP stimulates kinase activity of DG2P1 (EC(50): 0. Read More

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September 2004

Redesign of artificial globins: effects of residue replacements at hydrophobic sites on the structural properties.

Biochemistry 2000 May;39(19):5683-90

The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Artificial sequences of the 153 amino acids have been designed to fit the main-chain framework of the sperm whale myoglobin (Mb) structure based on a knowledge-based 3D-1D compatibility method. The previously designed artificial globin (DG1) folded into a monomeric, compact, highly helical and globular form with overall dimensions similar to those of the target structure, but it lacked structural uniqueness at the side-chain level [Isogai, Y., Ota, M. Read More

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Metabolism of digoxin and digoxigenin digitoxosides in rat liver microsomes: involvement of cytochrome P4503A.

Xenobiotica 1999 Feb;29(2):171-85

Department of Biopharmaceutical Sciences, School of Pharmacy, University of California, San Francisco 94143-0446, USA.

1. The sequential metabolism of digoxin (Dg3) to digoxigenin bis-digitoxoside (Dg2), digoxigenin mono-digitoxoside (Dg1) and digoxigenin (Dg0) was investigated in rat liver microsomes. 2. Read More

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February 1999

Evolution of visual pigments in geckos.

FEBS Lett 1999 Feb;445(1):36-40

Department of Earth and Space Science, Graduate School of Science, Osaka University, Japan.

Most geckos are nocturnal forms and possess rod retinas, but some diurnal genera have pure-cone retinas. We isolated cDNAs encoding the diurnal gecko opsins, dg1 and dg2, similar to nocturnal gecko P521 and P467, respectively. Despite the large morphological differences between the diurnal and nocturnal gecko photoreceptor types, they express phylogenetically closely related opsins. Read More

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February 1999

The validity of two quality assessment instruments: Monitor and Senior Monitor.

Int J Nurs Stud 1996 Dec;33(6):660-8

Department of Nursing Studies, King's College London, U.K.

This paper is concerned with validity of Monitor and Senior Monitor. The study carried out, which was funded by the Department of Health, attempted to establish the validity of the instruments through a multiple triangulation research design. Monitor and Senior Monitor were compared to another instrument, Qualpacs, and also to other methods that focused on quality of nursing care--observation of patients' activities and interactions with nurses, and interviews with patients and nurses on their perceptions of quality. Read More

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December 1996

Cantharidin-induced acantholysis: adhesion molecules, proteases, and related proteins.

Br J Dermatol 1994 Feb;130(2):148-57

Department of Dermatology, Churchill Hospital, Oxford, U.K.

Acantholysis is a feature of disorders such as Hailey-Hailey disease and Darier's disease. Immunocytochemical studies have shown internalization of desmosomal components after acantholysis. Basal cytokeratins show suprabasal expression in lesional Darier's disease. Read More

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February 1994

Estimates of variances due to direct and maternal effects for growth traits of Romanov sheep.

J Anim Sci 1993 Apr;71(4):845-9

Department of Animal Science, University of Nebraska, Lincoln 68583-0908.

Records of growth traits of 2,086 Romanov lambs were used to estimate variance components for an animal model and genetic correlations between growth traits. Traits analyzed were birth weight (BWT), weaning weight (WW), 90-d weight (W90), and daily gain for the periods birth to weaning (DG1) and weaning to 90 d (DG2). Weaning was at approximately 40 d. Read More

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Desmosome biogenesis in the mouse preimplantation embryo.

Development 1991 Jun;112(2):527-39

Department of Biology, University of Southampton, UK.

The molecular processes underlying the formation of the first desmosomes in the mouse early embryo have been examined by immunocytochemical and biochemical techniques using antibody probes recognising desmosomal proteins 1 and 2 (dp1 + 2, desmoplakins), dp3 (plakoglobin), desmosomal glycoprotein 1 (dg1, desmoglein) and dg2 + 3 (desmocollins). Immunofluorescence labelling of staged intact embryos and synchronised cell clusters indicates that dp1 + 2, dg1 and dg2 + 3 are first detectable on the lateral membrane contact sites between trophectoderm cells in early cavitating blastocysts, coincident with the onset of desmosome formation as seen in ultrastructural preparations. Membrane localisation of these antigens is predominantly punctate in appearance, occurs after division to the 32-cell stage and appears to be coincident with blastocoele formation since non-cavitated embryos/cell clusters of equivalent age/cell cycle are usually unlabelled. Read More

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Cloning and sequence analysis of desmosomal glycoproteins 2 and 3 (desmocollins): cadherin-like desmosomal adhesion molecules with heterogeneous cytoplasmic domains.

J Cell Biol 1991 Apr;113(2):381-91

Cancer Research Campaign, University of Southampton, Southampton General Hospital, United Kingdom.

Desmosomal glycoproteins 2 and 3 (dg2 and 3) or desmocollins have been implicated in desmosome adhesion. We have obtained a 5.0-kb-long clone for dg3 from a bovine nasal epidermal lambda gt11 cDNA library. Read More

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An immunoelectron microscopic comparison of desmosomal constituents and hemidesmosomal ones originating from the same tissue of the same animal.

Cell Struct Funct 1991 Apr;16(2):175-83

Department of Biology, University of Yamanashi Medical School, Japan.

The molecular constituents of desmosomes and hemidesmosomes were compared by examining bovine muzzle epidermis under immunoelectron microscopy using a postembedding method, first with antibodies prepared to four desmosomal antigens (DP1/2, DP3, DG1, DG2/3), followed by protein A-gold (PAG) complexes. The four antibodies showed almost negative labeling at hemidesmosomes as compared with the labeling observed at the desmosomes in the same tissue. By counting the number of PAG particles/200 millimicrons at hemidesmosomes and desmosomes, the above qualitative observation was confirmed quantitatively. Read More

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An immunohistological study of desmosomes in Darier's disease and Hailey-Hailey disease.

Br J Dermatol 1991 Mar;124(3):242-51

Department of Dermatology, Slade Hospital, Oxford, U.K.

The immunocytochemical distribution of desmosomal components was determined in involved skin from eight patients with Darier's disease, five patients with Hailey-Hailey disease and two patients with transient acantholytic dermatosis as well as skin from four normal controls. Sections were stained using monoclonal antibodies to the desmosomal proteins dp1 and dp2 (desmoplakins) and the desmosomal glycoproteins dg1 (desmoglein), and dg2 and dg3 (desmocollins). There was normal expression of desmosomal proteins and glycoproteins at the periphery of the keratinocytes in the perilesional skin in Darier's disease, in Hailey-Hailey disease and in transient acantholytic dermatosis. Read More

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