257,535 results match your criteria development vitro


In vitro models of the canine digestive tract as an alternative to in vivo assays: Advances and current challenges.

ALTEX 2022 Jan 13. Epub 2022 Jan 13.

Université Clermont Auvergne, UMR 454 MEDIS UCA-INRAE, Clermont-Ferrand, France.

Dogs occupy a full place in the family and their well-being is of paramount importance to their owners. Digestion, a complex process involving physicochemical, mechanical, and microbial parameters, plays a central in maintaining animals healthy. As in vivo studies in dogs are more and more restricted by ethical, regulatory, societal, and cost reasons, an alternative option resides in the use of in vitro models simulating the different parts of the canine gastrointestinal tract. Read More

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January 2022

Continuum modeling of thrombus formation and growth under different shear rates.

J Biomech 2022 Jan 4;132:110915. Epub 2022 Jan 4.

Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands. Electronic address:

Obstruction of blood flow due to thrombosis is a major cause of ischemic stroke, myocardial infarction, and in severe cases, mortality. In particular, in blood wetting medical devices, thrombosis is a common reason for failure. The prediction of thrombosis by understanding signaling pathways using computational models, lead to identify the risk of thrombus formation in blood-contacting devices and design improvements. Read More

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January 2022

Signaling pathways modulated by miRNAs in breast cancer angiogenesis and new therapeutics.

Pathol Res Pract 2022 Jan 10;230:153764. Epub 2022 Jan 10.

Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

MicroRNAs (miRNAs) act as oncogenes or tumor suppressors by suppressing the expression of target genes, some of which are engaged in angiogenic signaling pathways directly or indirectly. Tumor development and metastasis are dependent on angiogenesis, and it is the main reason for the poor prognosis of cancer patients. New blood vessels are formed from pre-existing vessels when angiogenesis occurs. Read More

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January 2022

The role of junctional adhesion molecule-C in trophoblast differentiation and function during normal pregnancy and preeclampsia.

Placenta 2022 Jan 10;118:55-65. Epub 2022 Jan 10.

Department of Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China. Electronic address:

Introduction: Junctional adhesion molecule-C (JAM-C) is an important regulator of many physiological processes, ranging from maintenance of tight junction integrity of epithelia to regulation of cell migration, homing and proliferation. Preeclampsia (PE) is a trophoblast-related syndrome with abnormal placentation and insufficient trophoblast invasion. However, the role of JAM-C in normal pregnancy and PE pathogenesis is unknown. Read More

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January 2022

A pro-endocrine pancreatic islet transcriptional program established during development is retained in human gallbladder epithelial cells.

Cell Mol Gastroenterol Hepatol 2022 Jan 12. Epub 2022 Jan 12.

Diabetes and Islet Biology Group, School of Medicine, Western Sydney University, 30 Narellan Road & Gilchrist Drive, Campbelltown, NSW 2560, Australia. Electronic address:

Background And Aims: Pancreatic islet β-cells are factories for insulin production; however ectopic expression of insulin is also well recognized. The gallbladder is a next-door neighbour to the developing pancreas. Here, we wanted to understand if gallbladders contain functional insulin-producing cells. Read More

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January 2022

Megakaryocyte-stromal cell interactions: effect on megakaryocyte proliferation, proplatelet production, and survival.

Exp Hematol 2022 Jan 12. Epub 2022 Jan 12.

Institute of Medical Research A Lanari, University of Buenos Aires, Buenos Aires, Argentina; Department of Experimental Hematology, Institute of Medical Research (IDIM), National Scientific and Technical Research Council (CONICET), University of Buenos Aires, Buenos Aires, Argentina. Electronic address:

Bone marrow stromal cells provide a proper environment for the development of hematologic lineages. The incorporation of different stromal cells to in vitro culture systems would be an attractive model to study megakaryopoiesis and thrombopoiesis. Our objective was to evaluate the participation of different types of stromal cells on in vitro megakaryopoiesis, thrombopoiesis and megakaryocyte (MK) survival. Read More

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January 2022

Purification and characterization of a novel thermostable anticoagulant protein from medicinal leech Whitmania pigra Whitman.

J Ethnopharmacol 2022 Jan 12:114990. Epub 2022 Jan 12.

Department of Chinese Materia Medica and Pharmacy, School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, 212013, PR China. Electronic address:

Ethnopharmacological Relevance: The prevalence of cardiovascular disease (CVD) is increasing worldwide. Despite significant improvements in novel targeted treatment agents, natural products purified from medicinal animals with minimal side effects have attracted much attention. Several native proteins explored from suck-blood leeches, such as non-thermostable hirudin and its variants, revealed potent anticoagulant activity. Read More

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January 2022

In situ polydopamine functionalized poly-L-lactic acid nanofibers with near-infrared-triggered antibacterial and reactive oxygen species scavenging capability.

Int J Biol Macromol 2022 Jan 12. Epub 2022 Jan 12.

School of Life Sciences, Nantong University, 226019 Nantong, China. Electronic address:

The development of a new multi-functional poly(L)-lactide (PLLA) nanofibrous scaffold with excellent antibacterial and reactive oxygen species (ROS) scavenging capability is quite important in tissue engineering. In this study, polydopamine (PDA)/PLLA nanofibers were prepared by combining electrospinning and post in-situ polymerization. The post in-situ polymerization of PDA on the PLLA nanofiber enable PDA uniformly distributed on PLLA nanofiber surface. Read More

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January 2022

2-Benzyllawsone protects against polymicrobial sepsis and vascular hyporeactivity in swiss albino mice.

Eur J Pharmacol 2022 Jan 12:174757. Epub 2022 Jan 12.

Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India. Electronic address:

Background: Novel naphthoquinone, 2-benzyllawsone (LT-9) was evaluated against vascular hyporeactivity and sepsis in cecal ligation and puncture (CLP) model in mice in view of its preliminary antibacterial and anti-inflammatory properties and to explore whether pretreatment with the molecule could restore vascular tone and contractile response to norepinephrine.

Methods: Evaluation of LT-9 against vascular hyporeactivity, hypotension, and sepsis-related inflammation and infection was carried out in the CLP model in Swiss albino mice and aortic smooth muscle cells in vitro.

Results: LT-9 showed potent reversal of the vascular hyporeactivity in CLP mice aorta. Read More

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January 2022

Antifungal lock therapy: an eternal promise or an effective alternative therapeutic approach?

Lett Appl Microbiol 2022 Jan 15. Epub 2022 Jan 15.

Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Hungary.

Each year, millions of central venous catheter insertions are performed in intensive care units worldwide. The usage of these indwelling devices is associated with a high risk of bacterial and fungal colonization, leading to the development of microbial consortia, namely biofilms. These sessile structures provide fungal cells with resistance to the majority of antifungals, environmental stress and host immune responses. Read More

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January 2022

Tc-labeled peptide targeting interleukin 13 receptor α 2 for tumor imaging in a cervical cancer mouse model.

Ann Nucl Med 2022 Jan 15. Epub 2022 Jan 15.

Department of Nuclear Medicine, Peking University First Hospital, No.8, Xishiku Street, Xicheng District, Beijing, 100034, China.

Objective: Pep-1 (CGEMGWVRC) can potently bind to interleukin 13 receptor α 2 (IL-13Rα2), a tumor-restricted receptor found to be expressed in various malignancies. In this study, we intended to prepare a Tc-labeled probe and evaluate its in vivo tumor accumulation properties in a cervical cancer xenograft model.

Methods: The Pep-1 was designed and radiolabeled with Tc by conjugation with mercaptoacetyl-triglycine (MAG). Read More

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January 2022

A semi in vivo pollination technique to assess the level of gametophytic self-incompatibility and pollen tube growth in pear (Pyrus communis L.).

Plant Reprod 2022 Jan 15. Epub 2022 Jan 15.

Laboratory for Plant Genetics and Crop Improvement, Division of Crop Biotechnics, Department of Biosystems, University of Leuven, 3000, Leuven, Belgium.

Key Message: We describe a semi in vivo pollination technique to determine the compatibility relation between different pear cultivars. This assay provides a valuable addition to existing tools in GSI research. The gametophytic self-incompatibility (GSI) system in Pyrus inhibits fertilization by pollen that shares one of the two S-alleles of the style. Read More

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January 2022

Aptamer-Functionalized Fractal Nanoplasmonics-Assisted Laser Desorption/Ionization Mass Spectrometry for Metabolite Detection.

Chempluschem 2022 Jan;87(1):e202100479

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P. R. China.

Accurate profiling of metabolites in biofluids provides a feasible approach to disease diagnosis and therapeutic evaluation in clinics. However, current material-based analytical platforms for in vitro metabolite detection often requires tedious sample pretreatment. To address this issue, herein, we report an aptamer-functionalized fractal gold nanoflower (Apt-AuNF)-based sensing platform for metabolite analysis. Read More

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January 2022

Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants.

Nat Commun 2022 Jan 14;13(1):27. Epub 2022 Jan 14.

Neuroscience and Mental Health Research Institute, Cardiff University, Cardiff, CF24 4HQ, UK.

Coordinated programs of gene expression drive brain development. It is unclear which transcriptional programs, in which cell-types, are affected in neuropsychiatric disorders such as schizophrenia. Here we integrate human genetics with transcriptomic data from differentiation of human embryonic stem cells into cortical excitatory neurons. Read More

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January 2022

Transient coupling between infragranular and subplate layers to Layer 1 neurons before ear opening and throughout the critical period depends on peripheral activity.

J Neurosci 2022 Jan 6. Epub 2022 Jan 6.

Department of Biology, University of Maryland, College Park, MD 20742

Cortical layer 1 (L1) contains a diverse population of interneurons which can modulate processing in superficial cortical layers, but the intracortical sources of synaptic input to these neurons, and how these inputs change over development and with sensory experience, is unknown. We here investigated the changing intracortical connectivity to L1 in the primary auditory cortex (A1) of mice of both sexes in slices across development using laser-scanning photostimulation. Before postnatal day (P)10, L1 cells receive excitatory input from within L1, L2/3, L4, and L5/6 as well as from subplate. Read More

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January 2022

Synthesis, radiolabeling, and evaluation of a potent β-site APP cleaving enzyme (BACE1) inhibitor for PET imaging of BACE1 in vivo.

Bioorg Med Chem Lett 2022 Jan 11:128543. Epub 2022 Jan 11.

Department of Nuclear Medicine, Laboratory of Clinical Nuclear Medicine, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address:

The β-site APP-cleaving enzyme 1 (BACE1) plays important roles in the proteolytic processing of amyloid precursor protein, and can be regarded as an important target for the diagnosis and treatment of AD. This study aimed to report the synthesis and evaluation of an F-labeled 2-amino-3,4-dihydroquinazoline analog as a potential BACE1 radioligand. A fluoropropyl side chain was introduced to the phenyl of this 3,4-dihydroquinazoline scaffold to generate the radioligand. Read More

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January 2022

Engineering in vitro immune-competent tissue models for testing and evaluation of therapeutics.

Adv Drug Deliv Rev 2022 Jan 11:114111. Epub 2022 Jan 11.

Fralin Biomedical Research Institute and Department of Biomedical Engineering and Mechanics, Virginia Tech, Roanoke, Virginia 24016, USA.

Advances in 3D cell culture, microscale fluidic control, and cellular analysis have enabled the development of more physiologically-relevant engineered models of human organs with precise control of the cellular microenvironment. Engineered models have been used successfully to answer fundamental biological questions and to screen therapeutics, but these often neglect key elements of the immune system. There are immune elements in every tissue that contribute to healthy and diseased states. Read More

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January 2022

EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3.

Mol Cancer 2022 Jan 14;21(1):16. Epub 2022 Jan 14.

Department of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, 107 Wenhua Western Road; Jinan, Shandong 250012, China, Jinan, 250012, Shandong, China.

Background: Gliomas are the most common malignant primary brain tumours with a highly immunosuppressive tumour microenvironment (TME) and poor prognosis. Circular RNAs (circRNA), a newly found type of endogenous noncoding RNA, characterized by high stability, abundance, conservation, have been shown to play an important role in the pathophysiological processes and TME remodelling of various tumours.

Methods: CircRNA sequencing analysis was performed to explore circRNA expression profiles in normal and glioma tissues. Read More

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January 2022

Down-regulation of the brain-specific cell-adhesion molecule contactin-3 in tuberous sclerosis complex during the early postnatal period.

J Neurodev Disord 2022 Jan 15;14(1). Epub 2022 Jan 15.

Department of (Neuro) Pathology, Amsterdam UMC, University of Amsterdam, Amsterdam Neuroscience, Amsterdam, the Netherlands.

Background: The genetic disorder tuberous sclerosis complex (TSC) is frequently accompanied by the development of neuropsychiatric disorders, including autism spectrum disorder and intellectual disability, with varying degrees of impairment. These co-morbidities in TSC have been linked to the structural brain abnormalities, such as cortical tubers, and recurrent epileptic seizures (in 70-80% cases). Previous transcriptomic analysis of cortical tubers revealed dysregulation of genes involved in cell adhesion in the brain, which may be associated with the neurodevelopmental deficits in TSC. Read More

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January 2022

Circular RNA hsa_circ_0032683 inhibits the progression of hepatocellular carcinoma by sponging microRNA-338-5p.

Bioengineered 2022 Feb;13(2):2321-2335

Department of Hepatic-Biliary-Pancreatic Center, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.

Recently, several studies have been conducted on circRNA (circular RNA). circRNA regulates gene expression and plays a vital role in the occurrence and development of various tumors. However, the role and mechanism of hsa_circ_0032683 in hepatocellular carcinoma (HCC) is not studied yet. Read More

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February 2022

Drug library screen identifies inhibitors of toxic astrogliosis.

Mult Scler Relat Disord 2022 Jan 4;58:103499. Epub 2022 Jan 4.

National Institute of Allergy and Infectious Diseases(NIAID), Neuroimmunological Diseases Section (NDS), National Institutes of Health(NIH), Building 10, Room 5N248, 10 Center Drive, MSC1444, Bethesda, MD 20892, USA. Electronic address:

Background: Multiple sclerosis (MS) is a chronic neuroinflammatory disorder, in which activated immune cells directly or indirectly induce demyelination and axonal degradation. Inflammatory stimuli also change the phenotype of astrocytes, making them neurotoxic. The resulting 'toxic astrocyte' phenotype has been observed in animal models of neuroinflammation and in MS lesions. Read More

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January 2022

Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.

Pathol Res Pract 2021 Dec 5;229:153734. Epub 2021 Dec 5.

Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China. Electronic address:

Clear cell renal cell carcinoma (ccRCC) is recognized as one of the most lethal malignancies among the urological system, with constantly increasing mortality. While the molecular mechanisms underlying ccRCC progression are still poorly understood, the molecular and functional role of lncRNA in multiple diseases has been well demonstrated. In this study, we hypothesized that lncRNA MEG8 might participate in ccRCC development. Read More

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December 2021

Inflammatory level under different p53 mutation status and the regulation role of curcumin in tumor microenvironment.

Immunobiology 2022 Jan 7;227(2):152177. Epub 2022 Jan 7.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China; Guizhou Provincial Key Laboratory & Drug Development on Common Disease, School of Basic Medicine, Guizhou Medical University, Guiyang, Guizhou, China. Electronic address:

The inflammation is tightly associated with tumor development, promoting or inhibiting tumorigenesis. And mutant p53 is speculated to promote inflammation and tumorigenesis. The tumor associated macrophages are usually educated to present the anti-inflammatory profile to tune down antitumor immunity. Read More

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January 2022

Induction of Cellular Senescence in Rat Vaginal Fibroblasts and Treatment With Senolytics: An in Vitro Model for the Study of Pelvic Organ Prolapse.

Female Pelvic Med Reconstr Surg 2022 Jan 12. Epub 2022 Jan 12.

From the Department of Obstetrics and Gynecology, Division of Female Pelvic Medicine and Reconstructive Surgery Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX.

Objective: The objective of this study was to develop an in vitro model of cellular senescence using rat vaginal fibroblasts and determine the effects of treatment with senolytics.

Methods: Rat vaginal tissue biopsies were collected. Primary vaginal fibroblasts were isolated and characterized by immunofluorescence. Read More

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January 2022

Human embryos in a dish - modeling early embryonic development with pluripotent stem cells.

Cell Regen 2022 Jan 14;11(1). Epub 2022 Jan 14.

Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences Research Triangle Park, Durham, NC, 27709, USA.

Stem cell-based embryo models present new opportunities to study early embryonic development. In a recent study, Kagawa et al. identified an approach to create human pluripotent stem cell-based blastoids that resemble the human blastocysts. Read More

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January 2022

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads.

Development 2022 Jan 14. Epub 2022 Jan 14.

Matière et Systèmes Complexes, UMR 7057 associée au CNRS et à l'Université de Paris, 10 rue Alice Domon et Léonie Duquet, 75013 Paris, France.

To investigate the role of mechanical constraints in morphogenesis and development, we develop a pipeline of techniques based on incompressible elastic sensors. These techniques combine the advantages of incompressible liquid droplets, which have been used as precise in situ shear stress sensors, and of elastic compressible beads, which are easier to tune and to use. Droplets of a polydimethylsiloxane (PDMS) mix, made fluorescent through specific covalent binding to a rhodamin dye, are produced by a microfluidics device. Read More

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January 2022

Effects of Bonito Elastin HC on Skin Dryness, Wrinkles, and Pigmentation and .

J Med Food 2022 Jan;25(1):48-60

Division of Food and Nutrition and Research Institute for Human Ecology, Chonnam National University, Gwangju, Korea.

We investigated the effects of bonito fish () elastin HC (KE) on skin dryness, wrinkles, and pigmentation and . , we evaluated the expression of mRNA genes and proteins related to skin dryness, wrinkles, and pigmentation. HaCaT and HS27 cells were exposed to ultraviolet B radiation (UVB) (50 mJ/cm), and B16F10 cells were stimulated with 3-isobutyl-1-methylxanthine (IBMX, 250 μg/mL) for 72 h to induce melanin synthesis. Read More

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January 2022

Beneficial Effects of a Combination of L. and Against Beta-Amyloid Peptide-Induced Neurodegeneration in Mice.

J Med Food 2022 Jan;25(1):12-23

Department of Biochemistry and Molecular Biology, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea.

Both (CL) and Tanaka (CJ) have been used to treat various diseases due to their anti-inflammatory and antioxidative stress activities. In this study, we investigated the ameliorative effect of the combination of CL extract and CJ extract (CCC) against beta-amyloid (A) peptide-induced neurological damage. CCC prevented neurocytotoxicity . Read More

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January 2022

Schisandrin B suppresses osteosarcoma lung metastasis by inhibiting the activation of the Wnt/β‑catenin and PI3K/Akt signaling pathways.

Oncol Rep 2022 Mar 14;47(3). Epub 2022 Jan 14.

Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.

Osteosarcoma (OS) is the most common malignant bone tumor worldwide and is associated with a poor prognosis, often being accompanied by lung metastasis at an early stage. At present, there are several side‑effects associated with the OS clinical treatment of OS, with the treatment effects often being unsatisfactory. Thus, there is an urgent need for the development of safe and effective novel drugs for the treatment of OS. Read More

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Vaccinium myrtillus L. extract associated with octocrylene, bisoctrizole and titanium dioxide: in vitro and in vivo tests to evaluate safety and efficacy.

J Cosmet Dermatol 2022 Jan 13. Epub 2022 Jan 13.

Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.

Objective: The harmful effects induced by ultraviolet exposition and the significant increment in skin cancer diagnosis confirm the necessity to develop effective and safe sunscreens. Limited efficacy and cutaneous adverse reactions of traditional formulations drove the incorporation of natural extracts into multifunctional sunscreens. Vaccinium myrtillus L. Read More

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January 2022