334,074 results match your criteria development identified

Development and validation of the Soweto Stress Scale: Mixed-methods, population-based study of adults living in Soweto, South Africa.

Soc Sci Med 2021 May 12;281:114023. Epub 2021 May 12.

SAMRC Developmental Pathways for Health Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; Edmund A. Walsh School of Foreign Service, Georgetown University, Washington, DC, USA. Electronic address:

This article moves from ethnography to epidemiology to generate a locally specific assessment of stress and evaluates it correlates with general psychiatric morbidity. We conducted two related ethnographic studies of stress and distress in Soweto, South Africa (N=107). We then used these studies to develop the Soweto Stress Scale, piloted the scale, and then evaluated its use in an epidemiological surveillance study of stress and health (N=933). Read More

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Identifying key areas of imbalanced supply and demand of ecosystem services at the urban agglomeration scale: A case study of the Fujian Delta in China.

Sci Total Environ 2021 Jun 5;791:148173. Epub 2021 Jun 5.

School of Architecture, Tianjin University, Tianjin 300072, China.

The coordinated supply and demand of ecosystem services (ESs) is important for ensuring regional sustainable development. However, research identifying key areas of imbalanced supply and demand of ESs at the urban agglomeration scale is limited. Therefore, in this study, using the Fujian Delta urban agglomeration of China as the research area, based on multi-source data, and analysis tools, such as ArcGIS, ENVI, and GeoDa, we constructed a research framework and indicator system for ESs supply and demand to determine the spatial change law, matching degree, and coupling coordination degree (CCD) of the ESs. Read More

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Illness stage and predominant polarity in bipolar disorder: Correlation with burden of illness and moderation of treatment outcome.

J Psychiatr Res 2021 Jun 2;140:205-213. Epub 2021 Jun 2.

Dauten Family Center for Bipolar Treatment Innovation, Department of Psychiatry, Massachusetts General Hospital, 50 Staniford Street, Suite 580, Boston, MA, 02114, United States; Harvard Medical School, 25 Shattuck Street, Boston, MA, 02115, United States. Electronic address:

Bipolar disorder often follows a set progression best described in stages where advanced stages are associated with poorer outcomes. Bipolar disorder is also often characterized by a predominance of episode polarity, where some individuals experience more depressive episodes (termed predominant depressive polarity) while others experience more hypo/manic episodes (termed predominant hypo/manic polarity). We examined the associations between staging and predominant polarity with measures of illness burden and treatment outcome utilizing data from a six-month comparative effectiveness trial of lithium and quetiapine in bipolar disorder (Bipolar CHOICE). Read More

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Maternal smoking during pregnancy and risks to depression and anxiety in offspring: An observational study and genome-wide gene-environment interaction analysis in UK biobank cohort.

J Psychiatr Res 2021 May 27;140:149-158. Epub 2021 May 27.

Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, China. Electronic address:

Background: Maternal smoking during pregnancy (MSDP) has been reported to be associated with increased anxiety and depression behaviors in offspring. However, there is still scant evidence to support the link between MSDP and anxiety/depression.

Methods: Using the subjects from the UK Biobank cohort (n = 371,903-432,881). Read More

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When a house is never a home: Housing instability among youth affected by parental drug abuse.

Child Abuse Negl 2021 Jun 9;118:105131. Epub 2021 Jun 9.

Johns Hopkins Bloomberg School of Public Health, Department of Population, Family and Reproductive Health, 615 North Wolfe Street, Baltimore, MD 21215, USA.

Background: The negative consequences of parental substance abuse are significant and longstanding for children. Among other risks, these youth are more likely to experience housing instability. The most common predictor of a child not living with their biological parent is parental substance use. Read More

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7-ketocholesterol: effects on viral infections and hypothetical contribution in COVID-19.

J Steroid Biochem Mol Biol 2021 Jun 9:105939. Epub 2021 Jun 9.

University Bourgogne Franche-Comté, Team 'Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism' EA 7270 / Inserm, 21000 Dijon, France. Electronic address:

7-ketocholesterol, which is one of the earliest cholesterol oxidization products identified, is essentially formed by the auto-oxidation of cholesterol. In the body, 7-ketocholesterol is both provided by food and produced endogenously. This pro-oxidant and pro-inflammatory molecule, which can activate apoptosis and autophagy at high concentrations, is an abundant component of oxidized Low Density Lipoproteins. Read More

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Compctutational identification of putative common genomic drug and vaccine targets in Mycoplasma genitalium.

Genomics 2021 Jun 9. Epub 2021 Jun 9.

Department of Immunology, Microbiology and Parasitology, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil. Electronic address:

Mycoplasma genitalium is an obligate intracellular bacterium that is responsible for several sexually transmitted infections, including non-gonococcal urethritis in men and several inflammatory reproductive tract syndromes in women. Here, we applied subtractive genomics and reverse vaccinology approaches for in silico prediction of potential vaccine and drug targets against five strains of M. genitalium. Read More

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Genome sequence of the cardiopulmonary canid nematode Angiostrongylus vasorum reveals species-specific genes with potential involvement in coagulopathy.

Genomics 2021 Jun 9. Epub 2021 Jun 9.

Institute of Parasitology, Vetsuisse-Faculty, University of Zurich, Winterthurerstrasse 266a, Zurich 8057, Switzerland.

Angiostrongylus vasorum is an emerging parasitic nematode of canids and causes respiratory distress, bleeding, and other signs in dogs. Despite its clinical importance, the molecular toolbox allowing the study of the parasite is incomplete. To address this gap, we have sequenced its nuclear genome using Oxford nanopore sequencing, polished with Illumina reads. Read More

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Identification and profiles of microRNAs in different development stages of miniature pig secondary palate.

Genomics 2021 Jun 9. Epub 2021 Jun 9.

Department of geriatric dentistry, Capital Medical University School of Stomatology, Tiantan Xili No.4, Beijing 100050, China; Laboratory of Molecular Signaling and Stem Cells Therapy, Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tiantan Xili No.4, Beijing 100050, China. Electronic address:

Cleft palate is one of the most frequent craniofacial malformation birth defects. Miniature pigs (Sus scrofa) are a valuable alternative large animal model to explore human palate development. Presently, the microRNA (miRNA) expression profiles in miniature pigs during palatogenesis from embryonic day (E) 30 to 50 were identified. Read More

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B cells acquire a unique and differential transcriptomic profile during pregnancy.

Genomics 2021 Jun 9. Epub 2021 Jun 9.

Center for Pharmacological and Botanical Studies (CEFYBO-UBA-CONICET), Medical Faculty, Buenos Aires University, Buenos Aires, Argentina; Centro Integrativo de Biología Y Química Aplicada, Universidad Bernardo O'Higgins, 8307993 Santiago, Chile. Electronic address:

Pregnancy alters B cell development and function. B cell activation is initiated by antigens binding to the BCR leading to B cell survival, proliferation, antigen presentation and antibody production. We performed a genome-wide transcriptome profiling of splenic B cells from pregnant (P) and non-pregnant (NP) mice and identified 1136 genes exhibiting differential expression in B cells from P mice (625 up- and 511 down-regulated) compared to NP animals. Read More

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Implementation of a Palliative Oncology Tumor Board.

J Pain Symptom Manage 2021 Jun 9. Epub 2021 Jun 9.

Penn Medicine Lancaster General Health Ann B Barshinger Cancer Institute, Lancaster, Pennsylvania, USA.

Context: The integration of palliative care into standard oncologic care has been shown to improve multiple outcomes in patients with advanced cancer. Ideal methods for integrating these disciplines is an ongoing area of discussion. One method of integration is a palliative oncology tumor board (POTB). Read More

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Anti-seizure activity of African medicinal plants: the identification of bioactive alkaloids from the stem bark of Rauvolfia caffra using an in vivo zebrafish model.

J Ethnopharmacol 2021 Jun 9:114282. Epub 2021 Jun 9.

Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria, South Africa, 0001. Electronic address:

Ethnopharmacological Relevance: Epilepsy is one of the major chronic diseases that does not have a cure to date. Adverse drug reactions have been reported from the use of available antiepileptic drugs (AEDs) which are also effective in only two-thirds of the patients. Accordingly, the identification of scaffolds with promising anti-seizure activity remains an important first step towards the development of new anti-epileptic therapies, with improved efficacy and reduced adverse effects. Read More

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Exploring the alternative: fish, flies and worms as preclinical models for ALS.

Neurosci Lett 2021 Jun 9:136041. Epub 2021 Jun 9.

KU Leuven - University of Leuven, Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute (LBI), Leuven, Belgium; VIB, Center for Brain & Disease Research, Laboratory of Neurobiology, Leuven, Belgium. Electronic address:

Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disorder characterized by the loss of upper and lower motor neurons. In general, patients succumb to respiratory insufficiency due to respiratory muscle weakness. Despite many promising therapeutic strategies primarily identified in rodent models, patient trials remain rather unsuccessful. Read More

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Light chain subunit of a poorly soluble human IgG2λ crystallizes in physiological pH environment both in cellulo and in vitro.

Biochim Biophys Acta Mol Cell Res 2021 Jun 9:119078. Epub 2021 Jun 9.

Department of Therapeutic Discovery, Amgen Inc., Thousand Oaks, CA 91320, USA.

Prominent inclusion bodies can develop in the endoplasmic reticulum (ER) when overexpressed antibodies possess intrinsically high condensation propensities. These observations suggest that antibodies deemed to show notable solubility problems may reveal such characteristics preemptively in the form of ER-associated inclusion bodies during antibody overexpression. To define the relationships between solubility problems and inclusion body phenotypes, we investigated the biosynthesis of a model human IgG2λ that shows severe opalescence in an acidic formulation buffer yet retains high solubility at physiological pH. Read More

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Pralsetinib for RET fusion-positive non-small-cell lung cancer (ARROW): a multi-cohort, open-label, phase 1/2 study.

Lancet Oncol 2021 Jun 9. Epub 2021 Jun 9.

Department of Investigational Cancer Therapeutics, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Background: Oncogenic alterations in RET have been identified in multiple tumour types, including 1-2% of non-small-cell lung cancers (NSCLCs). We aimed to assess the safety, tolerability, and antitumour activity of pralsetinib, a highly potent, oral, selective RET inhibitor, in patients with RET fusion-positive NSCLC.

Methods: ARROW is a multi-cohort, open-label, phase 1/2 study done at 71 sites (community and academic cancer centres) in 13 countries (Belgium, China, France, Germany, Hong Kong, Italy, Netherlands, Singapore, South Korea, Spain, Taiwan, the UK, and the USA). Read More

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Identifying counties at risk of high overdose mortality burden during the emerging fentanyl epidemic in the USA: a predictive statistical modelling study.

Lancet Public Health 2021 Jun 9. Epub 2021 Jun 9.

Department of Medicine, University of California, San Diego, CA, USA.

Background: The emergence of fentanyl around 2013 represented a new, deadly stage of the opioid epidemic in the USA. We aimed to develop a statistical regression approach to identify counties at the highest risk of high overdose mortality in the subsequent years by predicting annual county-level overdose death rates across the contiguous USA and to validate our approach against observed overdose mortality data collected between 2013 and 2018.

Methods: We fit mixed-effects negative binomial regression models to predict overdose death rates in the subsequent year for 2013-18 for all contiguous state counties in the USA (ie, excluding Alaska and Hawaii). Read More

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Ensuring patient and public involvement in the transition to AI-assisted mental health care: A systematic scoping review and agenda for design justice.

Health Expect 2021 Jun 12. Epub 2021 Jun 12.

Surrey Heartlands Health and Care Partnership, Guildford and Waverley CCG, Guildford, UK.

Background: Machine-learning algorithms and big data analytics, popularly known as 'artificial intelligence' (AI), are being developed and taken up globally. Patient and public involvement (PPI) in the transition to AI-assisted health care is essential for design justice based on diverse patient needs.

Objective: To inform the future development of PPI in AI-assisted health care by exploring public engagement in the conceptualization, design, development, testing, implementation, use and evaluation of AI technologies for mental health. Read More

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Epigenetic clock and methylation studies in elephants.

Aging Cell 2021 Jun 12:e13414. Epub 2021 Jun 12.

Department of Biostatistics, Fielding School of Public Health, University of California, Los Angeles, CA, USA.

Age-associated DNA-methylation profiles have been used successfully to develop highly accurate biomarkers of age ("epigenetic clocks") in humans, mice, dogs, and other species. Here we present epigenetic clocks for African and Asian elephants. These clocks were developed using novel DNA methylation profiles of 140 elephant blood samples of known age, at loci that are highly conserved between mammalian species, using a custom Infinium array (HorvathMammalMethylChip40). Read More

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Letermovir in lung transplant recipients with cytomegalovirus infection: A retrospective observational study.

Am J Transplant 2021 Jun 12. Epub 2021 Jun 12.

Department of Internal Medicine V, University Hospital, LMU, Comprehensive Pneumology Center (CPC-M), German Center for Lung Research (DZL), Germany.

Letermovir is a new antiviral drug approved for the prophylaxis of CMV infection in allogeneic stem cell transplants. The aim of the study was to assess the therapeutic efficacy of letermovir in difficult to treat CMV infections in lung transplant recipients. All lung transplant recipients between March 2018 and August 2020, who have been treated with letermovir for ganciclovir-resistant or refractory CMV infection were included in the study and analysed retrospectively. Read More

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The extracellular matrix proteins type I collagen, type III collagen, fibronectin, and laminin 421 stimulate migration of cancer cells.

FASEB J 2021 Jul;35(7):e21692

Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ), Heidelberg, Germany.

For metastasis formation, individual cells from a primary tumor must migrate toward other tissues. The aim of this study was to determine if mesenchymal stromal cells (MSCs) from human bone marrow are able to emit signals that induce this migratory activity in cancer cells. We separated the supernatant of MSCs derived from human bone marrow by size-exclusion and ion-exchange chromatography and have subsequently studied the migratory behavior of the prostate cancer cell line PC3 and the breast cancer cell line MDA-MB-231 toward the respective fractions in a transwell migration assay. Read More

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Split Green Fluorescent Protein-Based Contact Site Sensor (SPLICS ) for Heterotypic Organelle Juxtaposition as Applied to ER -Mitochondria Proximities.

Methods Mol Biol 2021 ;2275:363-378

Department of Biology, University of Padua, Padova, Italy.

In the last decades, membrane contact sites (MCSs) have been the object of intense investigation in different fields of cell physiology and pathology and their importance for the correct functioning of the cell is now widely recognized. MCS between any known intercellular organelles, including endoplasmic reticulum (ER), mitochondria, Golgi, endosomes, peroxisomes, lysosomes, lipid droplets, and the plasma membrane (PM), have been largely documented and in some cases the molecules responsible for the tethering also identified. They represent specific membrane hubs where a tightly coordinated exchange of ions, lipids, nutrients, and factors required to maintain proper cellular homeostasis takes place. Read More

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January 2021

Oxidative formation of bis-N-methylquinolinone from anti-head-to-head N-methylquinolinone cyclodimer.

Photochem Photobiol Sci 2021 Jun 12. Epub 2021 Jun 12.

Department of Chemistry, Philipps-Universität Marburg, Hans-Meerwein-Straße 4, 35032, Marburg, Germany.

The light-driven formation and cleavage of cyclobutane structural motifs resulting from [2 + 2]-pericyclic reactions, as found in thymine and coumarin-type systems, is an important and intensively studied photochemical reaction. Various applications are reported utilizing these systems, among others, in cross-linked polymers, light-triggered drug release, or other technical applications. Herein coumarin is most frequently used as the photoactive group. Read More

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Coverage with evidence development schemes for medical devices in Europe: characteristics and challenges.

Eur J Health Econ 2021 Jun 12. Epub 2021 Jun 12.

Centre for Health Economics, University of York, York, UK.

Objectives: Medical devices are potentially good candidates for coverage with evidence development (CED) schemes, as clinical data at market entry are often sparse and (cost-)effectiveness depends on real-world use. The objective of this research was to explore the diffusion of CED schemes for devices in Europe, and the factors that favour or hamper their utilization.

Methods: We conducted structured interviews with 25 decision-makers from 22 European countries to explore the characteristics of existing CED programmes for devices, and how decision makers perceived 13 pre-identified challenges associated with initiating and operating CED schemes for devices. Read More

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Treg cells in atherosclerosis.

Mol Biol Rep 2021 Jun 12. Epub 2021 Jun 12.

Department of Translational Research, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.

Atherosclerosis involves both innate and adaptive immunity. Here, we provide an overview of the role of regulatory T (Treg) cells in atherosclerotic diseases. Treg cells and their inhibitory cytokines, IL-10 and TGF-β, have been identified in atherosclerotic lesions and to inhibit progression through lipoprotein metabolism modulation. Read More

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Identification of key genes in the tumor microenvironment of lung adenocarcinoma.

Med Oncol 2021 Jun 12;38(7):83. Epub 2021 Jun 12.

Key Laboratory of Medical Imaging and Artifical Intelligence of Hunan Province, 423000, Chenzhou, P. R. China.

The tumor microenvironment plays an important role in tumor development and progression, but the role of immune and stromal cells in this environment has not been sufficiently studied. In this study, we aimed to identify key genes associated with the microenvironment of lung adenocarcinoma (LUAD). Raw data for stromal and immune cells in malignant tumors were downloaded from The Cancer Genome Atlas (TCGA). Read More

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Disparate genetic variants associated with distinct components of cowpea resistance to the seed beetle Callosobruchus maculatus.

Theor Appl Genet 2021 Jun 12. Epub 2021 Jun 12.

Department of Biology, Utah State University, Logan, UT, 84322, USA.

Key Message: Polygenic genome-wide association mapping identified two regions of the cowpea genome associated with different components of resistance to its major post-harvest pest, the seed beetle Callosobruchus maculatus. Cowpea (Vigna unguiculata) is an important grain and fodder crop in arid and semi-arid regions of Africa, Asia, and South America, where the cowpea seed beetle, Callosobruchus maculatus, is a serious post-harvest pest. Development of cultivars resistant to C. Read More

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Development and application of the Faba_bean_130K targeted next-generation sequencing SNP genotyping platform based on transcriptome sequencing.

Theor Appl Genet 2021 Jun 12. Epub 2021 Jun 12.

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Key Message: Large-scale faba bean transcriptome data are available, and the first genotyping platform based on liquid-phase probe targeted capture technology was developed for genetic and molecular breeding studies. Faba bean (Vicia faba L., 2n = 12) is an important food legume crop that is widely grown for multiple uses worldwide. Read More

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IP3-mediated Ca2+ signals regulate larval to pupal transition under nutrient stress through the H3K36 methyltransferase Set2.

Development 2021 Jun 12;148(11). Epub 2021 Jun 12.

National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India.

Persistent loss of dietary protein usually signals a shutdown of key metabolic pathways. In Drosophila larvae that have reached a 'critical weight' and can pupariate to form viable adults, such a metabolic shutdown would needlessly lead to death. Inositol 1,4,5-trisphosphate-mediated calcium (IP3/Ca2+) release in some interneurons (vGlutVGN6341) allows Drosophila larvae to pupariate on a protein-deficient diet by partially circumventing this shutdown through upregulation of neuropeptide signaling and the expression of ecdysone synthesis genes. Read More

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Exploring the sustainability of age-friendly initiatives in a Canadian province.

Gerontologist 2021 Jun 12. Epub 2021 Jun 12.

Department of City Planning, University of Manitoba, Winnipeg, Canada.

Background And Objectives: Based on the WHO Age-friendly Cities and Communities (AFCC) framework, the government of Manitoba, Canada, launched a province-wide age-friendly initiative in 2008. The objective of this study was to explore the sustainability of the AFCC initiative more than a decade later. The study was guided by conceptualizations of sustainability as multi-dimensional and dynamic, composed of four aspects (continued capacity; institutionalization; continued benefits; and development/adaptation), and an ecologic perspective that highlights the importance of contextual influences, and their change over time, on AFCC activities. Read More

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Immune correlates of therapy outcomes in women with cervical cancer treated with chemoradiotherapy: A systematic review.

Cancer Med 2021 Jun 12. Epub 2021 Jun 12.

Departments of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Background: Immune markers have been correlated with prognosis in a variety of solid tumors, including cervical cancer.

Objective: To review the literature on hematologic and immune markers and their association with recurrence and survival among patients with cervical cancer treated with chemoradiation.

Evidence Review: This systematic review was conducted in accordance with PRISMA guidelines via searches of Ovid MEDLINE, Ovid Embase, and the Cochrane Library using keywords regarding cervical cancer, immune markers, and HIV. Read More

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