3,267,215 results match your criteria development


Fluorescent probes for imaging bioactive species in subcellular organelles.

Chem Commun (Camb) 2021 Oct 27. Epub 2021 Oct 27.

Department of Chemical Sciences, Indian Institute of Science Education and Research, Kolkata, Mohanpur, 741 246, West Bengal, India.

Luminescent molecular probes and nanoscale materials have become important tools in biosensing and bioimaging applications because of their high sensitivity, fast response, specificity, and methodological simplicity. In recent years, there has been a notable advancement in fluorescent probes that respond to the subtle changes in subcellular microenvironments (, polarity, pH, and viscosity) or distribution of certain crucial biomarkers (, reactive oxygen species, ions, amino acids, and enzymes). The dynamic fluctuations of these bio-molecules in subcellular microenvironments control cellular homeostasis, immunity, signal conduction, and metabolism. Read More

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October 2021

Low-dimensional material based wearable sensors.

Nanotechnology 2021 Oct 27. Epub 2021 Oct 27.

Key Laboratory of MEMS of the Ministry of Education, Southeast University, Sipailou 2, Nanjing, Jiangsu, 210096, CHINA.

Wearable sensors are believed to be the most important part of the Internet of Things. In order to meet the application requirements, low-dimensional materials such as graphene and carbon nanotubes have been attempted to constitute wearable sensors with high performance. Our discussions in this review include the different low-dimensional material based sensors which are employed in wearable applications. Read More

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October 2021

Directed stimulation with interfascicular interfaces for peripheral nerve stimulation.

J Neural Eng 2021 Oct 27. Epub 2021 Oct 27.

Department of Biomedical Engineering, Case Western Reserve University, Wickenden 101, 10900 Euclid Ave, Cleveland, Ohio, 44106, UNITED STATES.

Objective: Computational models have shown that directional electrical contacts placed within the epineurium, between the fascicles, and not penetrating the perineurium, can achieve selectivity levels similar to point source contacts placed within the fascicle. The objective of this study is to test, in a murine model, the hypothesis that directed interfascicular contacts are selective.

Approach: Multiple interfascicular electrodes with directional contacts, exposed on a single face, were implanted in the sciatic nerves of thirty-two rabbits. Read More

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October 2021

Influence of salinity on the toxicity of copper and cadmium to Zebrafish embryos.

Aquat Toxicol 2021 Oct 20;241:106003. Epub 2021 Oct 20.

Centre for Environmental and Marine Studies (CESAM), Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.

Salinization has become a serious worldwide environmental perturbation in freshwater ecosystems. Concomitantly, many of such ecosystems are already impacted by other toxicants, which together with increased salinity may result in synergistic, antagonistic or additive toxic effects to biota. This work intended to assess the influence of increasing salinity (by using NaCl) on the lethal and sublethal toxicity of two metallic elements (copper and cadmium) in embryos of the fish species Danio rerio. Read More

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October 2021

Tartrazine exposure results in histological damage, oxidative stress, immune disorders and gut microbiota dysbiosis in juvenile crucian carp (Carassius carassius).

Aquat Toxicol 2021 Oct 15;241:105998. Epub 2021 Oct 15.

Engineering Lab of Henan Province for Aquatic Animal Disease Control, Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, Xinxiang 453007, PR China. Electronic address:

Tartrazine (TZ) is an azo dye widely used in foods, cosmetics, beverages, textile, and leather. In recent years, there are reports on detecting azo dyes in the aquatic environment, so the impact of these compounds on aquatic organisms could not be ignored. In this study, we aimed to evaluate the adverse effects of TZ exposure on teleosts' embryo development and juvenile's health by using crucian carp (Carassius carassius) as the experimental fish. Read More

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October 2021

Fetal surgery has no additional effects to general anesthesia on brain development in neonatal rabbits.

Am J Obstet Gynecol MFM 2021 Oct 24:100513. Epub 2021 Oct 24.

Department of Development and Regeneration, Cluster Woman and Child, Group Biomedical Sciences, KU Leuven, Belgium; Clinical Department Obstetrics and Gynaecology, University Hospitals Leuven, Leuven, Belgium; Institute for Women's Health, University College London, London, UK. Electronic address:

Background: Fetal surgery is part of modern fetal medicine, and some procedures such as fetal spina bifida repair are performed under general anesthesia. Fetuses are operated on in a time window when the developing brain is extremely vulnerable to external, potentially harmful factors. Until now, little is known if, and how open fetal surgery impacts fetal brain development. Read More

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October 2021

Novel Imaging Biomarker Prediction of Parenchymal Hemorrhage after Mechanical Thrombectomy in Patients with Large Ischemic Core.

J Stroke Cerebrovasc Dis 2021 Oct 24;31(1):106125. Epub 2021 Oct 24.

Department of Neurology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, 158 Shangtang Road, Hangzhou, Zhejiang 310014, China. Electronic address:

Background: Recently studies have shown that select acute ischemic stroke (AIS) patients with large ischemic core could be deemed as reasonable candidates to receive mechanical thrombectomy (MT) with low risk of developing parenchymal hemorrhage (PH) or symptomatic intracerebral hemorrhage (sICH); however, the selection criterion remains lacking. Our study aims to investigate the relationship between a novel imaging biomarker of largest core mass volume (LCMV) and development of PH in stroke patients with large ischemic core who have undergone MT.

Methods: A total of 26 AIS patients with large ischemic core (defined as ischemic core volume ≧ 50 ml) were enrolled in the study. Read More

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October 2021

Development of Physiologically Based Pharmacokinetic Model for Pregabalin to Predict the Pharmacokinetics in Pediatric Patients with Renal Impairment and Adjust Dosage Regimens.

J Pharm Sci 2021 Oct 24. Epub 2021 Oct 24.

Department of Pharmacy, the First Affiliated Hospital of Fujian Medical University, 20 Cha Zhong M. Rd, Fuzhou 350005, People's Republic of China, Telephone: 86-591-8798 1331, Fax: 86-591-8798 1331. Electronic address:

Pregabalin (PGB) is widely used clinically;however, its pharmacokinetics (PK) has not been studied in pediatric patients with renal impairment (RI). To design optimized PGB regimens for pediatric patients with varying degrees of RI and predict exposure to PGB, physiologically based pharmacokinetic (PBPK) models of PGB were developed and verified,and its dispositionwas simulated in the healthy population and adults with RI. The simulated results from the PBPK models after single-dose and multi-dose administrations of PGB were consistent with the corresponding observed data based on the fold error values of less than 2. Read More

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October 2021

Dose-based radiomic analysis (dosiomics) for intensity-modulated radiotherapy in patients with prostate cancer: Correlation between planned dose distribution and biochemical failure.

Int J Radiat Oncol Biol Phys 2021 Aug 5. Epub 2021 Aug 5.

Radiation Oncology Department, Cancer Institute Hospital, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku, Tokyo, 135-8550, Japan; AI Medical Center, Cancer Institute Hospital, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku, Tokyo, 135-8550, Japan.

Purpose: Although radiotherapy is one of the most significant modalities for localized prostate cancer, the prognostic factors for biochemical recurrence (BCR) regarding the treatment plan are unclear. We aimed to develop a novel dosiomics-based prediction model for BCR in patients with prostate cancer and clarify the correlations between the dosimetric factors and BCR.

Methods And Materials: This study included 489 patients with localized prostate cancer (BCR: 96, No-BCR: 393) who received intensity-modulated radiation therapy. Read More

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Monoclonal antibodies protect aged rhesus macaques from SARS-CoV-2-induced immune activation and neuroinflammation.

Cell Rep 2021 Oct 19:109942. Epub 2021 Oct 19.

Center for Immunology and Infectious Diseases, University of California, Davis, Davis, CA 95616, USA; California National Primate Research Center, University of California, Davis, Davis, CA 95616, USA; Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USA. Electronic address:

Anti-viral monoclonal antibody (mAb) treatments may provide immediate but short-term immunity from coronavirus disease 2019 (COVID-19) in high-risk populations, such as people with diabetes and the elderly; however, data on their efficacy in these populations are limited. We demonstrate that prophylactic mAb treatment blocks viral replication in both the upper and lower respiratory tracts in aged, type 2 diabetic rhesus macaques. mAb infusion dramatically curtails severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-mediated stimulation of interferon-induced chemokines and T cell activation, significantly reducing development of interstitial pneumonia. Read More

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October 2021

Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth.

Cell Chem Biol 2021 Oct 22. Epub 2021 Oct 22.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Haike Road, Shanghai 201210, China. Electronic address:

Rapamycin is widely recognized as an inhibitor of mTOR, and has been approved for clinical use as an immunosuppressant. Its potencies in anti-cancer, anti-aging, and neurodegenerative diseases are emergingly established. The exploration of other targets of rapamycin will further elucidate its underlying mechanisms of action. Read More

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October 2021

Novel in vitro invariant natural killer T cell functional assays.

J Immunol Methods 2021 Oct 24:113171. Epub 2021 Oct 24.

Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Canada; Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya; Department of Community Health Sciences, University of Manitoba, Winnipeg, Canada; Partners for Health and Development in Africa, Nairobi, Kenya. Electronic address:

Background: Invariant Natural Killer T (iNKT) cells are innate lymphocytes bridging the innate and adaptive immune systems and are critical first responders against cancer and infectious diseases. iNKT cell phenotype and functionality are studied using in vitro stimulation assays assessing cytokine response and proliferation capabilities. The most common stimulant is the glycolipid α-Galactosyl Ceramide (α-GalCer), which stimulates iNKT cells when presented by CD1d, an MHC class I-like molecule expressed by antigen-presenting cells (APC). Read More

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October 2021

The Arabidopsis mature endosperm promotes seedling cuticle formation via release of sulfated peptides.

Dev Cell 2021 Oct 22. Epub 2021 Oct 22.

Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland; Institute of Genetics and Genomics in Geneva (iGE3), University of Geneva, Geneva, Switzerland. Electronic address:

In Arabidopsis mature seeds, the onset of the embryo-to-seedling transition is nonautonomously controlled, being blocked by endospermic abscisic acid (ABA) release under unfavorable conditions. Whether the mature endosperm governs additional nonautonomous developmental processes during this transition is unknown. Mature embryos have a more permeable cuticle than seedlings, consistent with their endospermic ABA uptake capability. Read More

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October 2021

New options for bloodstream infection caused by colistin- or ceftazidime/avibactam resistant Klebsiella pneumoniae.

Int J Antimicrob Agents 2021 Oct 24:106458. Epub 2021 Oct 24.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

The concerns regarding carbapenem-resistant Klebsiella pneumoniae (CR-Kp) especially in bloodstream infection (BSI) are continuing to increase worldwide. Several novel agents with activity against BSI CR-Kp have been approved or are in late-stage clinical development. Here, the antibacterial effects of ceftazidime/avibactam (CZA), aztreonam/avibactam (AZA), meropenem/vaborbactam (MEV), imipenem-cilastatin/relebactam (ICR), eravacycline (ERV) against three colistin- or four CZA resistant CR-Kp (COLR-Kp or CZAR-Kp) were tested using time-kill assays. Read More

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October 2021

Pathogenic role of mitogen activated protein kinases in protozoan parasites.

Biochimie 2021 Oct 24. Epub 2021 Oct 24.

Division of Biochemistry and Structural Biology, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, 226031, Uttar Pradesh, India. Electronic address:

Protozoan parasites with complex life cycles have high mortality rates affecting billions of human lives. Available anti-parasitic drugs are inadequate due to variable efficacy, toxicity, poor patient compliance and drug-resistance. Hence, there is an urgent need for the development of safer and better chemotherapeutics. Read More

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October 2021

Development and Usability Testing of a Mobile Application to Monitor Patient-Reported Outcomes after Stress Urinary Incontinence Surgery.

Urology 2021 Oct 24. Epub 2021 Oct 24.

Department of Healthcare Policy and Research, Weill Cornell Medical College-New York Presbyterian, New York, NY, USA.

Objectives: To develop and perform a usability testing of a mobile application (app) with representative users of surgeons and female patients undergoing stress urinary incontinence (SUI) surgery.

Methods: A mobile app was developed with the Medical Device Epidemiology Network (MDEpiNet) High-Performance Integrated Virtual Environment (HIVE) to streamline the collection of patient-reported outcomes following SUI surgery using validated questionnaires. It was designed as a collaborative effort with the Women's Health Initiative (WHI), including patient and surgeon involvement. Read More

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October 2021

Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis.

Cell Rep 2021 Oct;37(4):109885

Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA. Electronic address:

Sertoli cells are highly polarized testicular supporting cells that simultaneously nurture multiple stages of germ cells during spermatogenesis. Proper localization of polarity protein complexes within Sertoli cells, including those responsible for blood-testis barrier formation, is vital for spermatogenesis. However, the mechanisms and developmental timing that underlie Sertoli cell polarity are poorly understood. Read More

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October 2021

Conserved heterodimeric GTPase Rbg1/Tma46 promotes efficient translation in eukaryotic cells.

Cell Rep 2021 Oct;37(4):109877

Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA; Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA; Carl R. Woese Institute for Genomic Biology, 1206 West Gregory Drive, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA. Electronic address:

Conserved developmentally regulated guanosine triphosphate (GTP)-binding proteins (Drgs) and their binding partner Drg family regulatory proteins (Dfrps) are important for embryonic development, cellular growth control, differentiation, and proliferation. Here, we report that the yeast Drg1/Dfrp1 ortholog Rbg1/Tma46 facilitates translational initiation, elongation, and termination by suppressing prolonged ribosome pausing. Consistent with the genome-wide observations, deletion of Rbg1 exacerbates the growth defect resulting from translation stalling, and Rbg1 stabilizes mRNAs against no-go decay. Read More

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October 2021

P5A ATPase controls ER translocation of Wnt in neuronal migration.

Cell Rep 2021 Oct;37(4):109901

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address:

The Wnt family contains conserved secretory proteins required for developmental patterning and tissue homeostasis. However, how Wnt is targeted to the endoplasmic reticulum (ER) for processing and secretion remains poorly understood. Here, we report that CATP-8/P5A ATPase directs neuronal migration non-cell autonomously in Caenorhabditis elegans by regulating EGL-20/Wnt biogenesis. Read More

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October 2021

An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.

Neuron 2021 Oct 19. Epub 2021 Oct 19.

Laboratory of Axonal Growth and Regeneration, German Center for Neurodegenerative Diseases (DZNE), Venusberg Campus 1/99, 53127 Bonn, Germany. Electronic address:

Axons in the adult mammalian central nervous system fail to regenerate after spinal cord injury. Neurons lose their capacity to regenerate during development, but the intracellular processes underlying this loss are unclear. We found that critical components of the presynaptic active zone prevent axon regeneration in adult mice. Read More

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October 2021

Bidirectional control of infant rat social behavior via dopaminergic innervation of the basolateral amygdala.

Neuron 2021 Oct 20. Epub 2021 Oct 20.

Department of Child and Adolescent Psychiatry, NYU Langone Health, New York, NY 10016, USA; Emotional Brain Institute, Nathan Kline Institute for Psychiatric Research, Orangeburg, NY 10962, USA; Center for Neural Science, New York University, New York, NY 10003, USA. Electronic address:

Social interaction deficits seen in psychiatric disorders emerge in early-life and are most closely linked to aberrant neural circuit function. Due to technical limitations, we have limited understanding of how typical versus pathological social behavior circuits develop. Using a suite of invasive procedures in awake, behaving infant rats, including optogenetics, microdialysis, and microinfusions, we dissected the circuits controlling the gradual increase in social behavior deficits following two complementary procedures-naturalistic harsh maternal care and repeated shock alone or with an anesthetized mother. Read More

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October 2021

Genetic characterization and zoonotic potential of Blastocystis from wild animals in Sichuan Wolong National Natural Reserve, Southwest China.

Parasite 2021 27;28:73. Epub 2021 Oct 27.

Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China.

Blastocystis is a prevalent eukaryotic parasite that has been identified in a wide range of hosts. Several species are considered potential sources of Blastocystis infection in humans, but little is known about the prevalence of Blastocystis in wild animals. In this study, the prevalence and subtypes of Blastocystis were investigated to assess the zoonotic potential of wild animals in Sichuan Wolong National Natural Reserve. Read More

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October 2021

Genetic factors controlling nTiO2 nanoparticles stress tolerance in barley (Hordeum vulgare) during seed germination and seedling development.

Funct Plant Biol 2021 Oct 28. Epub 2021 Oct 28.

Titanium dioxide nanoparticle (nTiO2) is one of the most produced nanoparticles worldwide. Its mechanism on crop development and performance is unclear as it is hard to predict their toxicity or benefit. Therefore, understanding the genetics of crop development under nTiO2 is a prerequisite for their applications in agriculture and crop improvement. Read More

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October 2021

Receptor Tyrosine Kinases ror1/2 and ryk Are Co-expressed with Multiple Wnt Signaling Components During Early Development of Sea Urchin Embryos.

Biol Bull 2021 Oct 1;241(2):140-157. Epub 2021 Oct 1.

AbstractA combination of receptors, co-receptors, and secreted Wnt modulators form protein complexes at the cell surface that activate one or more of the three different Wnt signaling pathways (Wnt/-catenin, Wnt/JNK, and Wnt/Ca). Two or more of these pathways are often active in the same cellular territories, forming Wnt signaling networks; however, the molecular mechanisms necessary to integrate information from these pathways in these situations are unclear in any model system. Recent studies have implicated two Wnt binding receptor tyrosine kinases, receptor tyrosine kinase-like orphan receptor (Ror) and related-to-receptor tyrosine kinase (Ryk), in the regulation of canonical and non-canonical Wnt signaling pathways, depending on the context; however, the spatiotemporal expression of these genes in relation to Wnt signaling components has not been well characterized in most deuterostome model systems. Read More

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October 2021

Chip-Scalable, Room-Temperature, Zero-Bias, Graphene-Based Terahertz Detectors with Nanosecond Response Time.

ACS Nano 2021 Oct 27. Epub 2021 Oct 27.

NEST, CNR - Istituto Nanoscienze and Scuola Normale Superiore, Piazza San Silvestro 12, 56127, Pisa, Italy.

The scalable synthesis and transfer of large-area graphene underpins the development of nanoscale photonic devices ideal for new applications in a variety of fields, ranging from biotechnology, to wearable sensors for healthcare and motion detection, to quantum transport, communications, and metrology. We report room-temperature zero-bias thermoelectric photodetectors, based on single- and polycrystal graphene grown by chemical vapor deposition (CVD), tunable over the whole terahertz range (0.1-10 THz) by selecting the resonance of an on-chip patterned nanoantenna. Read More

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October 2021

RNA and Protein Delivery By Cell-Secreted and Bioengineered Extracellular Vesicles.

Adv Healthc Mater 2021 Oct 27:e2101557. Epub 2021 Oct 27.

Department of Biomedical Engineering, 622 West 168th Street VC12-234, New York, 10032, USA.

Extracellular vesicles (EVs) are carriers of biological signals through export and delivery of RNAs and proteins. Of increasing interest is the use of EVs as a platform for delivery of biomolecules. Preclinical studies have effectively used EVs to treat a number of diseases. Read More

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October 2021

Co-occurrence of BAP1 and SF3B1 mutations in uveal melanoma induces cellular senescence.

Mol Oncol 2021 Oct 27. Epub 2021 Oct 27.

Department of Pharmacology and Moores Cancer Center, University of California, San Diego, La Jolla, CA, 92093, USA.

Uveal melanoma (UM) is the most common intraocular tumor in adults. Recurrent mutations in BRCA1-associated protein 1 (BAP1) and splicing factor 3B subunit 1 (SF3B1) display a mutually exclusive pattern in UM, but the underlying mechanism is unknown. We show that combined BAP1 deficiency and SF3B1 hotspot mutation leads to senescence and growth arrest in human UM cells. Read More

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October 2021

Country-level determinants of COVID-19 case rates and death rates: An ecological study.

Transbound Emerg Dis 2021 Oct 27. Epub 2021 Oct 27.

Department of Medicine, St. Elizabeth's Medical Center, Boston, MA, USA.

Introduction: The Coronavirus Disease 2019 (COVID-19) pandemic has had a variable worldwide impact, likely related to country-level characteristics. In this ecological study, we explored the association of COVID-19 case rates (per 100,000 people) and death rates (per 100,000 people) with country-level population health characteristics, economic and human development indicators, and habitat-related variables.

Methods: To calculate country-level COVID-19 case and death rates, the number of cases and deaths were extracted from the Johns Hopkins Coronavirus Resource Center through September 30, 2021. Read More

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October 2021