Plast Reconstr Surg 2021 Jun 21. Epub 2021 Jun 21.
From the Department of Orthopedic Surgery, Mayo Clinic; the Department of Plastic, Reconstructive, and Hand Surgery, Radboud University Medical Center; and the Department of Plastic, Reconstructive, and Hand Surgery, Erasmus Medical Center.
Background: Mesenchymal stem cells have the potential to produce neurotrophic growth factors and establish a supportive microenvironment for neural regeneration. The purpose of this study was to determine the effect of undifferentiated and differentiated mesenchymal stem cells dynamically seeded onto decellularized nerve allografts on functional outcomes when used in peripheral nerve repair.
Methods: In 80 Lewis rats, a 10-mm sciatic nerve defect was reconstructed with (1) autograft, (2) decellularized allograft, (3) decellularized allograft seeded with undifferentiated mesenchymal stem cells, or (4) decellularized allograft seeded with mesenchymal stem cells differentiated into Schwann cell-like cells. Read More