13,950 results match your criteria detection steps


Rapid LC-MS/MS method for the detection of seven animal species in meat products.

Food Chem 2021 Sep 17;371:131075. Epub 2021 Sep 17.

China Meat Research Center, 100068 Beijing, China. Electronic address:

The adulteration of meat products has been reported worldwide, and detection of specific peptides through mass spectrometry (MS) is a reliable method for meat species identification. However, the practical application of this method is limited by complicated steps and long reaction time of the traditional sample preparation. Therefore, this paper introduced a convenient and time-saving sample preparation by optimizing the steps of reduction, alkylation, digestion, and purification. Read More

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September 2021

Propagating Uncertainty Across Cascaded Medical Imaging Tasks For Improved Deep Learning Inference.

IEEE Trans Med Imaging 2021 Sep 20;PP. Epub 2021 Sep 20.

Although deep networks have been shown to perform very well on a variety of medical imaging tasks, inference in the presence of pathology presents several challenges to common models. These challenges impede the integration of deep learning models into real clinical workflows, where the customary process of cascading deterministic outputs from a sequence of image-based inference steps (e.g. Read More

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September 2021

A Novel Method to Map Small RNAs with High Resolution.

Bio Protoc 2021 Aug 20;11(16):e4128. Epub 2021 Aug 20.

Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA.

Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular molecule dynamics . However, the application of super-resolution imaging techniques to detect small RNAs (sRNAs) is limited by the choice of proper fluorophores, autofluorescence of samples, and failure to multiplex. Read More

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Automatic deep learning system for COVID-19 infection quantification in chest CT.

Multimed Tools Appl 2021 Sep 13:1-15. Epub 2021 Sep 13.

College of Engineering and Technology, American University of the Middle East, Egaila, Kuwait.

The paper proposes an automatic deep learning system for COVID-19 infection areas segmentation in chest CT scans. CT imaging proved its ability to detect the COVID-19 disease even for asymptotic patients, which make it a trustworthy alternative for PCR. Coronavirus disease spread globally and PCR screening is the adopted diagnostic testing method for COVID-19 detection. Read More

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September 2021

The Polyvalent Gold Nanoparticle Conjugate-Materials Synthesis, Biodiagnostics, and Intracellular Gene Regulation.

Authors:
Chad A Mirkin

MRS Bull 2010 Jul 31;35(7):532-539. Epub 2011 Jan 31.

International Institute for Nanotechnology.

Advances in nanoscale directed assembly strategies have enabled researchers to analogize atomic assembly via chemical reactions and nanoparticle assembly, creating a new nanoscale "periodic table." We are just beginning to realize the nanoparticle equivalents of molecules and extended materials and are currently developing the ground rules for creating programmable nanometer-scale coordination environments. The ability to create a diverse set of nanoscale architectures from one class of nanoparticle building blocks would allow for the synthesis of designer materials, wherein the physical properties of a material could be predicted and controlled a . Read More

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Material strategies and considerations for serologic testing of global infectious diseases.

MRS Bull 2021 Sep 13:1-5. Epub 2021 Sep 13.

National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD USA.

The SARS-CoV-2 pandemic has brought to light multiple considerations when approaching infectious diseases on the global level. These range from diagnostic platforms, to therapeutics, and prevention agents. In this article, we focus on the engineering platforms and considerations when applying serologic assays to multiple geographic locations, climates with varying endemic virus repertoires, and different laboratory and clinical resource settings. Read More

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September 2021

Compact automated capillary electrophoresis instrument for coupling with mass spectrometry by using sheathless electrospray ionization.

J Chromatogr A 2021 Sep 4;1656:462533. Epub 2021 Sep 4.

Department of Chemistry, University of Basel, Klingelbergstrasse 80, 4056 Basel, Switzerland. Electronic address:

The low-cost set-up is based on the flexible microfluidic breadboard approach. To avoid the use of electrically driven devices at the high voltage injection end of the capillary pneumatically actuated pumps and valves are employed. It is coupled to the mass spectrometer with a sheathless electrospray obtained in an electrodeless interface configuration. Read More

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September 2021

A simple colorimetric detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification assay using hydroxynaphthol blue metal indicator.

J Virol Methods 2021 Sep 15:114289. Epub 2021 Sep 15.

College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea. Electronic address:

A simple reverse transcription loop-mediated isothermal amplification combined with visual detection method (vRT-LAMP) assay was developed for rapid and specific detection of porcine epidemic diarrhea virus (PEDV) in this study, which overcomes the shortcomings of previously described RT-LAMP assays that require additional detection steps or pose a risk of cross-contamination. The assay results can be directly detected by the naked eye using hydroxynaphthol blue after incubating for 40 min at 62 °C. The assay specifically amplified PEDV RNA and no other viral nucleic acids. Read More

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September 2021

Detection of SARS-CoV-2 variant B.1.1.7 in a cat in Germany.

Res Vet Sci 2021 Sep 9;140:229-232. Epub 2021 Sep 9.

Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany. Electronic address:

Several non-variant of concern SARS-CoV-2 infections in pets have been reported as documented in the OIE and GISAID databases and there is only one fully documented case of an alpha variant of concern (VOC)(B.1.1. Read More

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September 2021

A simple and sensitive aptasensor with rolling circle amplification for viable Cronobacter sakazakii detection in powdered infant formula.

J Dairy Sci 2021 Sep 13. Epub 2021 Sep 13.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, PR China. Electronic address:

Cronobacter sakazakii is a foodborne, emerging opportunistic pathogen that causes severe bacteremia, necrotizing enterocolitis, and sepsis with a mortality rate of up to 80%. In this study, we developed a simple and sensitive fluorescent turn-off aptasensor with rolling circle amplification assay for viable C. sakazakii detection in powdered infant formula. Read More

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September 2021

A DNAzyme-catalyzed label-free aptasensor based on multifunctional dendrimer-like DNA assembly for sensitive detection of carcinoembryonic antigen.

Biosens Bioelectron 2021 Sep 10;194:113618. Epub 2021 Sep 10.

Department of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, 450001, China; Henan Key Laboratory of Molecular Pathology, Zhengzhou, 450001, China.

Carcinoembryonic antigen (CEA) is an important malign tumor marker. In this study, a simple, label-free and antibody-free aptasensor was fabricated based on a multifunctional dendrimer-like DNA nanoassembly. The DNA nanoassembly was embedded with multiple G-quadruplex DNAzyme motifs and a hanging CEA aptamer motif. Read More

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September 2021

Green porous benzamide-like nanomembranes for hazardous cations detection, separation, and concentration adjustment.

J Hazard Mater 2021 Sep 11;423(Pt B):127130. Epub 2021 Sep 11.

Department of Polymer Technology, Faculty of Chemistry, Gdańsk University of Technology, G. Narutowicza 11/12, 80-233 Gdańsk, Poland.

Green biomaterials play a crucial role in the diagnosis and treatment of diseases as well as health-related problem-solving. Typically, biocompatibility, biodegradability, and mechanical strength are requirements centered on biomaterial engineering. However, in-hospital therapeutics require an elaborated synthesis of hybrid and complex nanomaterials capable of mimicking cellular behavior. Read More

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September 2021

Screening ovarian cancers with Raman spectroscopy of blood plasma coupled with machine learning data processing.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 4;265:120355. Epub 2021 Sep 4.

School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

The mortality of ovarian cancer is closely related to its poor rate of early detection. In the search of an efficient diagnosis method, Raman spectroscopy of blood features as a promising technique allowing simple, rapid, minimally-invasive and cost-effective detection of cancers, in particular ovarian cancer. Although Raman spectroscopy has been demonstrated to be effective to detect ovarian cancers with respect to normal controls, a binary classification remains idealized with respect to the real clinical practice. Read More

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September 2021

A Sensitive Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method Based on Derivatization with 1-Nitro-2-Naphthaldehyde for Determination of Alkylhydrazines in Surface water.

J AOAC Int 2021 Sep 16. Epub 2021 Sep 16.

Daejeon-Sejong Division, Korea Occupational Safety and Health Agency, Daejeon City, Republic of Korea.

Background: Alkylhydrazines are widely used in the industrial fields. An analysis of alkylhydrazines in surface water is need because these chemicals are likely to be discharged into wastewater and enter aquatic environments.

Objective: An ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to determine the levels of five alkylhydrazines (N,N-dimethylhydrazine, ethylhydrazine, 1-isopropylhydrazine, phenylhydrazine and 1-methyl-1-phenylhydrazine) in surface water. Read More

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September 2021

Rapid and sensitive leukemia-derived exosome quantification nicking endonuclease-assisted target recycling.

Anal Methods 2021 Sep 16;13(35):4001-4007. Epub 2021 Sep 16.

School of Life Sciences, Anhui Medical University, Hefei, Anhui 230032, China.

Exosomes as fluid biomarkers hold great promise for noninvasive cancer diagnosis. However, a method for the rapid and convenient detection of exosomes is still a challenge because current analysis processes involve multiple steps and yield low sensitivity. Here, we developed a wash-free fluorescent biosensor for the rapid and sensitive quantification of exosomes by combining aptamer and nicking endonuclease (Nb·BbvCI). Read More

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September 2021

ReactionDataExtractor: A Tool for Automated Extraction of Information from Chemical Reaction Schemes.

J Chem Inf Model 2021 Sep 15. Epub 2021 Sep 15.

Cavendish Laboratory, Department of Physics, University of Cambridge, J. J. Thomson Avenue, Cambridge CB3 0HE, U.K.

Chemical reaction schemes are commonly used for visual encapsulation of chemical information. Figures of reaction schemes contain chemical transformations, the chemical species involved, as well as reaction conditions. From a data-mining point of view, they constitute rich sources, densely packed with knowledge. Read More

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September 2021

Optimization and validation of capillary electrophoresis- and gas chromatography-tandem mass spectrometry methods for the analysis of intermediate metabolites in glycolysis and pentose phosphate pathways within biological samples.

J Chromatogr A 2021 Sep 4;1656:462531. Epub 2021 Sep 4.

Integrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute, Seoul 03759, Republic of Korea; Department of Chenistry and Nano Science, Ewha Womans University, Seoul 03760, Republic of Korea. Electronic address:

Highly selective methods for the analysis of intermediate metabolites involved in glycolysis and phosphate pentose pathways are essential for metabolism and metabolic flux studies. However, the successful separation of phosphorylated compounds is difficult due to their high polarity, as well as their structural isomers. In this study, phosphorylated compounds in spiked serum samples were analyzed using capillary electrophoresis tandem mass spectrometry (CE-MS/MS) and gas chromatography (GC)-MS/MS. Read More

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September 2021

Improving turnaround times for HLA-B*27 and HLA-B*57:01 gene testing: a Barts Health NHS Trust quality improvement project.

BMJ Open Qual 2021 09;10(3)

Clinical Transplantation Laboratory, Barts Health NHS Trust, London, UK.

Among other tests, Barts Health NHS Trust clinical transplantation laboratory conducts two important gene-detection tests: human leucocyte antigen (HLA)-B*27 ('B27', associated with the diagnosis of ankylosing spondylitis) and HLA-B*57:01 ('B57', associated with prediction of abacavir hypersensitivity disorder). The turnaround time (TaT) from sample receipt to return of results is important to clinicians and their patients but was not monitored. Furthermore, we anticipated an imminent increase in demand from a forthcoming pathology service merger, together with long-term increases with the rise of personalised genetic medicine. Read More

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September 2021

Rapid and quantitative detection of SARS-CoV-2 IgG antibody in serum using optofluidic point-of-care testing fluorescence biosensor.

Talanta 2021 Dec 14;235:122800. Epub 2021 Aug 14.

School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China. Electronic address:

The COVID-19 pandemic brings unprecedented crisis for public health and economics in the world. Detecting specific antibodies to SARS-CoV-2 is a powerful supplement for the diagnosis of COVID-19 and is important for epidemiological studies and vaccine validations. Herein, a rapid and quantitative detection method of anti-SARS-CoV-2 IgG antibody was built based on the optofluidic point-of-care testing fluorescence biosensor. Read More

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December 2021

Transferable, easy-to-use and room-temperature-storable PCR mixes for microfluidic molecular diagnostics.

Talanta 2021 Dec 10;235:122797. Epub 2021 Aug 10.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen, 361102, China; School of Life Sciences, Xiamen University, Xiamen, 361102, China.

As the outbreak of coronavirus disease 2019 (COVID-19), on-site molecular diagnosis is becoming increasingly important. In this study, a freeze-drying method was introduced for PCR reagents to meet the requirements of microfluidic molecular diagnosis. Using this method, PCR components were pre-mixed and freeze-dried as a bead, which could be transferred into microfluidic chips easily. Read More

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December 2021

Production of full-length SARS-CoV-2 nucleocapsid protein from Escherichia coli optimized by native hydrophobic interaction chromatography hyphenated to multi-angle light scattering detection.

Talanta 2021 Dec 16;235:122691. Epub 2021 Jul 16.

Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), 1190 Vienna, Austria; acib - Austrian Centre of Industrial Biotechnology, 1190, Vienna, Austria.

The nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for several steps of the viral life cycle, and is abundantly expressed during infection, making it an ideal diagnostic target protein. This protein has a strong tendency for dimerization and interaction with nucleic acids. For the first time, high titers of NP were expressed in E. Read More

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December 2021

SERS liquid biopsy: An emerging tool for medical diagnosis.

Colloids Surf B Biointerfaces 2021 Aug 24;208:112064. Epub 2021 Aug 24.

Faculty of Physics, Babeș-Bolyai University, 400084, Cluj-Napoca, Romania; Biomed Data Analytics SRL, 400696, Cluj-Napoca, Romania. Electronic address:

Surface-enhanced Raman scattering (SERS) is emerging as a novel strategy for biofluid analysis. In this review, we delineate four experimental SERS protocols that are frequently used for the profiling of biofluids: 1) liquid SERS for the detection of purine metabolites; 2) iodide-modified liquid SERS for the detection of proteins; 3) dried SERS for the detection of both purine metabolites and proteins; 4) resonant Raman for the detection of carotenoids. To explain the selectivity of each experimental SERS protocol, we introduce a heuristic model for the chemisorption of analytes mediated by adsorbed ions (adions) onto the SERS substrate. Read More

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Acetonitrile adduct analysis of underivatised amino acids offers improved sensitivity for hydrophilic interaction liquid chromatography tandem mass-spectrometry.

J Chromatogr A 2021 Sep 3;1655:462530. Epub 2021 Sep 3.

School of Life Sciences, Faculty of Science, The University of Technology Sydney, Ultimo 2007, Australia. Electronic address:

LC-MS/MS method development for native amino acid detection can be problematic due to low ionisation efficiencies, in source fragmentation, potential for cluster ion formation and incorrect application of chromatography techniques. This has led to the majority of the scientific community derivatising amino acids for more sensitive analysis. Derivatisation has several benefits including reduced signal-to-noise ratios, more efficient ionisation, and a change in polarity, allowing the use of reverse phase chromatography. Read More

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September 2021

Early Detection of Symptom Exacerbation in Patients With SARS-CoV-2 Infection Using the Fitbit Charge 3 (DEXTERITY): Pilot Evaluation.

JMIR Form Res 2021 Sep 16;5(9):e30819. Epub 2021 Sep 16.

Department of Health Promotion and Medicine of the Future, Kanazawa University, Kanazawa, Japan.

Background: Some patients with COVID-19 experienced sudden death due to rapid symptom deterioration. Thus, it is important to predict COVID-19 symptom exacerbation at an early stage prior to increasing severity in patients. Patients with COVID-19 could experience a unique "silent hypoxia" at an early stage of the infection when they are apparently asymptomatic, but with rather low SpO (oxygen saturation) levels. Read More

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September 2021

Lab-on-a-Disc for Point-of-Care Infection Diagnostics.

Acc Chem Res 2021 Sep 13. Epub 2021 Sep 13.

Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.

ConspectusReliable, inexpensive, and rapid diagnostic tools are essential to control and prevent the spread of infectious diseases. Many commercial kits for coronavirus disease 2019 (COVID-19) diagnostics have played a crucial role in the fight against the COVID-19 pandemic. Most current standard diagnostic (IVD) protocols for infectious diseases are sensitive but time-consuming and require sophisticated laboratory equipment and specially trained personnel. Read More

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September 2021

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue.

J Vis Exp 2021 Aug 28(174). Epub 2021 Aug 28.

Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay;

The proteomic analysis of the human brain tissue over the last decade has greatly enhanced our understanding of the brain. However, brain related disorders continue to be a major contributor of deaths around the world, necessitating the need for even greater understanding of their pathobiology. Traditional antibody-based techniques like western blotting or immunohistochemistry suffer from being low-throughput besides being labor-intensive and qualitative or semi-quantitative. Read More

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Advanced data preprocessing for comprehensive two-dimensional gas chromatography with vacuum ultraviolet spectroscopy detection.

J Sep Sci 2021 Sep 12. Epub 2021 Sep 12.

IFP Energies nouvelles, Rond-point de l'échangeur de Solaize BP 3, Solaize, 69360, France.

Comprehensive two-dimensional gas chromatography with vacuum ultraviolet detection results in sizable data for which noise and baseline drift ought to be corrected. As the data is acquired from multiple channels, preprocessing steps have to be applied to the data from all channels while being robust and rather fast with respect to the significant size of the data. In this study, we have described advanced data preprocessing techniques for such data which were not available in the existing commercial software solutions and which were dedicated primarily to noise and baseline correction. Read More

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September 2021

Assessment of land use and land cover change detection and prediction using remote sensing and CA Markov in the northern coastal districts of Tamil Nadu, India.

Environ Sci Pollut Res Int 2021 Sep 12. Epub 2021 Sep 12.

Department of Civil Engineering, National Institute of Technology, Tiruchirappalli, Tamil Nadu, India.

The study on land use and land cover (LULC) changes assists in analyzing the change and regulates environment sustainability. Hence, this research analyzes the Northern TN coast, which is under both natural and anthropogenic stress. The analysis of LULC changes and LULC projections for the region between 2009-2019 and 2019-2030 was performed utilizing Google Earth Engine (GEE), TerrSet, and Geographical Information System (GIS) tools. Read More

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September 2021

Raman spectrum classification based on transfer learning by a convolutional neural network: Application to pesticide detection.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 7;265:120366. Epub 2021 Sep 7.

College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018 China. Electronic address:

Pesticide detection is of tremendous importance in agriculture, and Raman spectroscopy/Surface-Enhanced Raman Scattering (SERS) has proven extremely effective as a stand-alone method to detect pesticide residues. Machine learning may be able to automate such detection, but conventional algorithms require a complete database of Raman spectra, which is not feasible. To bypass this problem, the present study describes a transfer learning method that improves the algorithm's accuracy and speed to extract features and classify Raman spectra. Read More

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September 2021

A simple methodology for RNA isolation from bacteria by integration of formamide extraction and chitosan-modified silica purification.

Anal Bioanal Chem 2021 Sep 10. Epub 2021 Sep 10.

Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Chemistry and Molecular Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, Shandong, China.

RNA isolation from bacteria is technically difficult due to the RNA characteristic of labile and vulnerable degradation. Many reagents were explored for cellular lysis and complete inhibition of RNase. However, the available methods for RNA isolation are either of low efficiency or time-consuming. Read More

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September 2021