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Research Note: Detection of antibiotic-resistance genes in commercial poultry and turkey flocks from Italy.

Poult Sci 2021 Mar 2;100(5):101084. Epub 2021 Mar 2.

Faculty of Veterinary Medicine, University of Teramo, 64110 Teramo, Italy.

Antibiotics are routinely used in commercial poultry farms for the treatment of economically important bacterial diseases. Repeated use of antibiotics, usually administered in the feed or drinking water, may also result in the selection of resistant bacteria in animal feces, able to transfer their antimicrobial-resistance genes (ARG), residing on mobile elements, to other microorganisms, including human pathogens. In this study, single and multiplex PCR protocols were performed to detect tetracycline-, lincomycin-, chloramphenicol-, aminoglycoside-, colistin-, vancomycin-, and carbapenem-resistance genes, starting from 38 litter samples collected from 6 poultry and 2 turkey Italian flocks. Read More

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4-hour immuno-chromatographic detection of intestinal carriage of carbapenemase-producing : a validation study.

J Clin Microbiol 2021 Mar 31. Epub 2021 Mar 31.

Sorbonne Université APHP.6, GRC 29, DMU DREAM, Hôpital Tenon, Département d'Anesthésie-Réanimation et Médecine Périopératoire, Paris, France

The increasing incidence of Carbapenemase-Producing Gram-Negative Bacilli (C-PGNB) represents a major public health challenge. Rapid detection of the digestive colonization with C-PGNB is fundamental to control their spread. Validation of a rapid protocol for C-PGNB detection directly on rectal swabs. Read More

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Detection of colistin-resistant populations prior to antibiotic exposure in KPC-2-producing Klebsiella pneumoniae clinical isolates.

J Microbiol 2021 Mar 29. Epub 2021 Mar 29.

Department of Microbiology and Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea.

Although colistin is frequently regarded as the antibiotic of last resort in treating carbapenem-resistant Klebsiella pneumoniae, colistin heteroresistance may in part be associated with antibiotic treatment failure. However, we do not know how widespread the colistin heteroresistance is in carbapenem-resistant K. pneumoniae isolates. Read More

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Antimicrobial Susceptibility Profiles to Predict the Presence of Carbapenemase Genes among Carbapenem-Resistant .

J Clin Microbiol 2021 Mar 24. Epub 2021 Mar 24.

Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention.

Detection of carbapenem-resistant (CRPA) with carbapenamase-producing (CP) genes is critical for preventing transmission. Our objective was to assess whether certain antimicrobial susceptibility testing (AST) profiles can efficiently identify CP-CRPA. We defined CRPA as with imipenem or meropenem MICs of ≥8μg/ml; CP-CRPA were CRPA with CP genes (///). Read More

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Activity of cefepime/zidebactam (WCK 5222) against 'problem' antibiotic-resistant Gram-negative bacteria sent to a national reference laboratory.

J Antimicrob Chemother 2021 Mar 24. Epub 2021 Mar 24.

Antimicrobial Resistance and Healthcare-Associated Infections Reference Unit, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UK.

Background: Triple-action diazabicyclooctanes, e.g. zidebactam, combine β-lactamase inhibition, antibacterial activity, and 'enhancement' of PBP3-targeted β-lactams. Read More

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DETECTION OF β-LACTAMASE PRODUCING PROTEUS MIRABILIS OF ANIMAL ORIGIN IN ANDHRA PRADESH, INDIA AND THEIR GENETIC DIVERSITY.

J Food Prot 2021 Mar 16. Epub 2021 Mar 16.

MVSc student, Dept. of VPE, NTR CVSc, Gannavaram-521102, Krishna Dist, Andhra Pradesh, India.

Proteus mirabilis is abundantly found in soil and water, and although it is part of the normal human intestinal flora, it has been known to cause serious infections in humans and a common pathogen responsible for complicated urinary tract infections. It is also commonly associated with multidrug resistance. In the current study, analysis of 1093 different samples from foods of animal origin and intestinal samples confirmed 232 P. Read More

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Cefepime/tazobactam compared with other tazobactam combinations against problem Gram-negative bacteria.

Int J Antimicrob Agents 2021 Mar 11:106318. Epub 2021 Mar 11.

Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, National Infection Service, Public Health England, London, UK; Norwich Medical School, University of East Anglia, Norwich, UK. Electronic address:

Objectives: Piperacillin/tazobactam has long been a broad-spectrum 'workhorse' antibiotic; however, it is compromised by resistance. One response is to re-partner tazobactam with cefepime, which is easier to protect, being less β-lactamase labile, and to use a high-dose and prolonged infusion. On this basis, Wockhardt are developing cefepime/tazobactam (WCK 4282) as a 2+2 g q8h combination with a 90-min infusion. Read More

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Characterization of Carbapenem-Resistant ST15 Clone Coproducing KPC-2, CTX-M-15 and SHV-28 Spread in an Intensive Care Unit of a Tertiary Hospital.

Infect Drug Resist 2021 3;14:767-773. Epub 2021 Mar 3.

Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu Province, People's Republic of China.

Objective: Nosocomial infection caused by carbapenem-resistant (CRKP) is a great threat to severely ill patients. Here we report an outbreak of ST15 isolates co-producing KPC-2, CTX-M-15, and SHV-28 in the cardiac surgery intensive care unit (CSICU) of a tertiary hospital.

Materials And Methods: From November 2019 to August 2020, all non-duplicated CRKP isolates were collected from the CSICU. Read More

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Carbapenemase detection testing in the era of ceftazidime/avibactam-resistant KPC-producing Enterobacterales: A 2-year experience.

J Glob Antimicrob Resist 2021 Mar 20;24:411-414. Epub 2021 Feb 20.

Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, Corso Bramante 88/90, 10126 Turin, Italy.

Objectives: The aim of this study was to investigate the prevalence of ceftazidime/avibactam (CZA) resistance among carbapenemase-producing Enterobacterales (CPE) blood culture isolates as well as the performance of the main carbapenemase phenotypic detection methods to identify KPC variants associated with CZA resistance.

Methods: Non-duplicate CPE strains isolated from blood cultures during 2018-2020 were tested for antimicrobial susceptibility. Molecular testing was used to identify carbapenemase-producers. Read More

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Detection of carbapenemase-producing bacteria in a public healthcare center from Venezuela.

J Infect Dev Ctries 2020 Dec 27;15(1):163-167. Epub 2020 Dec 27.

School of Medicine, Medical Faculty, University of Zulia, Maracaibo, Venezuela.

Introduction: The dramatic increase in the prevalence and clinical impact of infections caused by Carbapenemase-Producing Bacteria in the nosocomial setting in Latin America represents an emerging challenge to public health. The present study detected carbapenemase-producing Gram-negative bacteria in patients from a Hospital from Venezuela, by phenotypic and genotypic methods.

Methodology: The bacterial identification was carried out using conventional methods. Read More

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December 2020

Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales.

Front Med (Lausanne) 2020 20;7:616490. Epub 2021 Jan 20.

Team "Resist" UMR1184 "Immunology of Viral, Auto-Immune, Hematological and Bacterial diseases (IMVA-HB)," INSERM, Université Paris-Saclay, CEA, LabEx LERMIT, Faculty of Medicine, Le Kremlin-Bicêtre, France.

Gram-negative bacteria, especially Enterobacterales, have emerged as major players in antimicrobial resistance worldwide. Resistance may affect all major classes of anti-gram-negative agents, becoming multidrug resistant or even pan-drug resistant. Currently, β-lactamase-mediated resistance does not spare even the most powerful β-lactams (carbapenems), whose activity is challenged by carbapenemases. Read More

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January 2021

Evaluation of the Immunochromatographic NG-Test Carba 5, RESIST-5 O.O.K.N.V., and IMP -SeT for Rapid Detection of KPC-, NDM-, IMP-, VIM-type, and OXA-48-like Carbapenemase Among .

Front Microbiol 2020 15;11:609856. Epub 2021 Jan 15.

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.

Background: are the most common pathogens for nosocomial infections. The emergence and spread of KPC, NDM, and OXA-48-like carbapenemase-producing with their extensively drug-resistant characteristics have posed great threats to public health. This study aimed to evaluate the performance of NG-test Carba 5, RESIST-5 O. Read More

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January 2021

Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly From Bronchoalveolar Lavage Fluid Spiked With spp.

Front Microbiol 2020 13;11:597684. Epub 2021 Jan 13.

Institute for Global Health (ISGlobal), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.

Carbapenem-resistant spp. mainly are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex SuperBug Complete A system, based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in spp. Read More

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January 2021

Detection of IncN-pST15 one-health plasmid harbouring bla in a hypermucoviscous Klebsiella pneumoniae CG258 isolated from an infected dog, Brazil.

Transbound Emerg Dis 2021 Jan 28. Epub 2021 Jan 28.

One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil.

The emergence and rapid spread of carbapenemase-producing Enterobacterales represents a serious public health concern. Critically, these global priority bacteria have begun to be reported in companion animals, implying a potential risk of cross-transmission between humans and pets. Using long-read (MinION) and short-read (Illumina) sequencing technologies, we have identified and characterized a hypermucoviscous KPC-2-producing Klebsiella pneumoniae strain belonging to the high-risk international clone ST11/CG258, in a dog with urinary tract infection. Read More

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January 2021

Comparing three different phenotypic methods for accurate detection of carbapenemase-producing Enterobacterales.

J Infect Chemother 2021 Jun 16;27(6):794-799. Epub 2021 Jan 16.

Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu, China. Electronic address:

Background: Early identification of carbapenemase-producing Enterobacterales (CPE) is highly essential to prevent their dissemination within health care settings.

Objective: This study aimed to compare 3 reported phenotypic assays for detecting carbapenemase-producing Enterobacterales (CPE).

Methods: 151 Enterobacterales isolates were collected, the sensitivity and specificity of each test was determined, with molecular genotype serving as the gold standard. Read More

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[Analysis of related factors of carbapenem resistant Klebsiella pneumoniae infection in patients with artificial airway].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Nov;32(11):1324-1330

Department of Critical Care Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China. Corresponding author: Chen Wei, Email:

Objective: To analyze the clinical characteristics and related influencing factors of carbapenem resistant Klebsiella pneumoniae (CRKP) in patients with artificial airway and mechanical ventilation in intensive care unit (ICU), and provide theoretical basis for clinical prevention of Klebsiella pneumoniae infection.

Methods: The clinical data of patients with pulmonary infection of Klebsiella pneumoniae in ICU of Beijing Shijitan Hospital Affiliated to Capital Medical University from January 2016 to December 2019 were collected. Compared CRKP pneumonia patients (study group) with carbapenem antibiotic sensitive Klebsiella pneumoniae (CSKP) pneumonia patients (control group), the clinical characteristics [gender, age, acute physiology and chronic health evaluation II (APACHE II), duration of mechanical ventilation, proportion of patients with mechanical ventilation > 10 days, use of antibiotics before detection of Klebsiella pneumoniae, white blood cell count (WBC), C-reactive protein (CRP), procalcitonin (PCT), albumin, bedridden for more than 1 month before admission, 28-day mortality] were analyzed. Read More

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November 2020

Novel multiplex PCRs for detection of the most prevalent carbapenemase genes in Gram-negative bacteria within Germany.

J Med Microbiol 2021 Mar 15;70(3). Epub 2021 Jan 15.

Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, 50923 Köln, Germany.

Gram-negative bacteria are a common source of infection both in hospitals and in the community, and antimicrobial resistance is frequent among them, making antibiotic therapy difficult, especially when these isolates carry carbapenem resistance determinants.. A simple method to detect all the commonly found carbapenemases in Germany was not available. Read More

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Carriage of Carbapenem-Resistant Enterobacterales in Adult Patients Admitted to a University Hospital in Italy.

Antibiotics (Basel) 2021 Jan 10;10(1). Epub 2021 Jan 10.

Department of Biomolecular Sciences, University of Urbino, 61029 Fano, Italy.

The emerging spread of carbapenemase-producing Enterobacterales (CPE) strains, in particular, and , has become a significant threat to hospitalized patients. Carbapenemase genes are frequently located on plasmids than can be exchanged among clonal strains, increasing the antibiotic resistance rate. The aim of this study was to determine the prevalence of CPE in patients upon their admission and to analyze selected associated factors. Read More

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January 2021

Waist-to-height ratio and BMI as predictive markers for insulin resistance in women with PCOS in Kolkata, India.

Endocrine 2021 Jan 5. Epub 2021 Jan 5.

Post Graduate Department of Physiology, Hooghly Mohsin College, University of Burdwan, Bardhaman, West Bengal, India.

Purpose: Polycystic ovarian syndrome (PCOS) is most commonly presented with insulin resistance (IR). Simple anthropometric indices may serve as surrogate markers of these conditions with population-based cut-off values. The present study suggests the cut-off values of waist-to-height ratio (WHtR) and body mass index (BMI) in early prediction of PCOS and IR in PCOS women based in Kolkata, a major metropolitan city in India. Read More

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January 2021

Rapid and accurate eXDR screening: use Xpert Carba-R® with FecalSwab®.

Diagn Microbiol Infect Dis 2021 Apr 26;99(4):115279. Epub 2020 Nov 26.

Service de Microbiologie Hygiène Hospitalière, Hôpital Bicêtre, Le Kremlin-Bicêtre, France; CNR de la Résistance Aux Antibiotiques, Hôpital Bicêtre, Le Kremlin-Bicêtre, France.

The FecalSwab® displays high performances for stool culture, but it was not assessed for carbapenemase-producing Enterobacterales (CPE) screening. We assess the performances of the Xpert Carba-R v2® with the FecalSwab®. Using a collection of 12 CPE strains, the limit of detection was assessed at 158 CFU/swab [interquartile range 93-589]. Read More

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Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEX.

Talanta 2021 Mar 8;224:121750. Epub 2020 Oct 8.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210046, China. Electronic address:

Klebsiella pneumoniae carbapenemase 2 (KPC-2) is a serine β-lactamase that can hydrolyze almost all β-lactam antibiotics. The drug resistant problem of bacteria expressing carbapenemases is currently a global problem, therefore, rapid and specific detection of pathogenic bacteria is urgent. In order to obtain an aptamer that can specifically recognize bacteria expressing KPC-2, we have established a method called Precision-SELEX. Read More

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Comparison of Genotypic and Phenotypic Methods of Metallo-β- lactamase Detection in spp.

J Glob Infect Dis 2020 Jul-Sep;12(3):141-143. Epub 2020 Aug 29.

Department of Orthopaedics, JPNA Trauma Centre, All India Institute of Medical Sciences, New Delhi, India.

Introduction: MBL containing genes have been reported in all GNBs including spp since 1990s which are worrisome as they are transmitted by mobile genetic elements. Thus, early detection of MBL encoding organisms is necessary. The current study was designed to identify the most sensitive cost-effective test which could be used as a screening test for detection of cabapenamase producing isolates. Read More

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Genomic Epidemiology of Carbapenemase Producing Strains at a Northern Portuguese Hospital Enables the Detection of a Misidentified KPC-3 Producing Strain.

Microorganisms 2020 Dec 13;8(12). Epub 2020 Dec 13.

Department of Microbiology and Immunology, Faculty of Pharmacy, Universidade de Lisboa, 1649-033 Lisboa, Portugal.

The evolutionary epidemiology, resistome, virulome and mobilome of thirty-one multidrug resistant clinical isolates from the northern Vila Real region of Portugal were characterized using whole-genome sequencing and bioinformatic analysis. The genomic population structure was dominated by two main sequence types (STs): ST147 ( = 17; 54.8%) and ST15 ( = 6; 19. Read More

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December 2020

Confronting Ceftolozane-Tazobactam Susceptibility in Multidrug-Resistant Enterobacterales Isolates and Whole-Genome Sequencing Results (STEP Study).

Int J Antimicrob Agents 2021 Feb 10;57(2):106259. Epub 2020 Dec 10.

Servicio de Microbiología. Hospital Universitario Ramón y Cajal e Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.

Ceftolozane-tazobactam (C/T) is frequently used for infections caused by multidrug-resistant (MDR)-Enterobacterales isolates. Whole-genome sequencing (WGS, Illumina-Hiseq 4000/NovaSeq 6000, OGC, UK) was used to study the population structure, the resistome and the virulome of C/T-susceptible and -resistant MDR Escherichia spp. (n=30) and Klebsiella spp. Read More

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February 2021

Detection of carbapenem-resistant Klebsiella pneumoniae strains harboring carbapenemase, beta-lactamase and quinolone resistance genes in intensive care unit patients.

GMS Hyg Infect Control 2020 30;15:Doc31. Epub 2020 Nov 30.

Beykent University School of Medicine, Department of Medical Microbiology, Istanbul, Turkey.

Carbapenem-resistant (CR-Kp) strains are important nosocomial pathogens worldwide. In this study, we aimed to reveal the antibiotic resistance of clinical CR-Kp strains and determine the presence of KPC, OXA-48, VIM and IMP carbapenemase genes. CTX-M-1, TEM-1, SHV-1 extended-spectrum beta-lactamase (ESBL) genes, , , plasmid-mediated quinolone resistance genes and sul1 and sul2 sulfonamide resistance genes provided molecular epidemiological data. Read More

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November 2020

Emergence of a Clinical Sequence Type 131 Strain Carrying a Chromosomal Gene.

Front Microbiol 2020 13;11:586764. Epub 2020 Nov 13.

Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Bacteria carrying the carbapenemase genes have rapidly spread worldwide and have become a great threat to public health. The gene has been primarily located on plasmids cocirculating in various strains. However, chromosomal integration of the gene in has not been reported. Read More

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November 2020

Evaluation of CARBA PAcE, a novel rapid test for detection of carbapenemase-producing .

J Med Microbiol 2021 Feb;70(2)

Institute for Medical Microbiology and Virology, University of Oldenburg, Oldenburg, Germany.

Carbapenemase-producing (CPE) are an increasing threat to global health. Fast detection is crucial for patient management and outbreak control. Recently, a new commercial colorimetric test, CARBA PAcE, was released that has not yet been scientifically evaluated. Read More

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February 2021

Identification of variable stages in murine pancreatic tumors by a multiparametric approach employing hyperpolarized C MRSI, H diffusivity and H T MRI.

NMR Biomed 2021 02 21;34(2):e4446. Epub 2020 Nov 21.

Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, Israel.

This study explored the usefulness of multiple quantitative MRI approaches to detect pancreatic ductal adenocarcinomas in two murine models, PAN-02 and KPC. Methods assayed included H T and T measurements, quantitative diffusivity mapping, magnetization transfer (MT) H MRI throughout the abdomen and hyperpolarized C spectroscopic imaging. The progress of the disease was followed as a function of its development; studies were also conducted for wildtype control mice and for mice with induced mild acute pancreatitis. Read More

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February 2021

Evaluation of three molecular carbapenemase tests: Eazyplex SuperBug complete B, Novodiag CarbaR+, and Amplidiag CarbaR+MCR.

J Microbiol Methods 2021 01 18;180:106105. Epub 2020 Nov 18.

Helsinki University and Helsinki University Hospital, HUSLAB, Department of Clinical Microbiology, Helsinki, Finland. Electronic address:

Background: Carbapenemase-producing Gram-negative bacilli, i.e., Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter, are of increased concern for the public health around the world. Read More

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January 2021