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Is Lupus Anticoagulant a Significant Feature of COVID-19? A Critical Appraisal of the Literature.

Semin Thromb Hemost 2021 Jun 15. Epub 2021 Jun 15.

Section of Clinical Biochemistry, University of Verona, Verona, Italy.

The term "lupus anticoagulant (LA)" identifies a form of antiphospholipid antibodies (aPLs) causing prolongation of clotting tests in a phospholipid concentration-dependent manner. LA is one of the laboratory criteria identified in patients with antiphospholipid (antibody) syndrome (APS). The presence of LA in patients with APS represents a significant risk factor for both thrombosis and pregnancy morbidity. Read More

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Toward a Liver Cell Atlas: Understanding Liver Biology in Health and Disease at Single-Cell Resolution.

Semin Liver Dis 2021 Jun 15. Epub 2021 Jun 15.

Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland.

Single-cell technologies are revolutionizing our understanding of cellular heterogeneity and functional diversity in health and disease. Here, we review the current knowledge and advances in liver biology using single-cell approaches. We focus on the landscape of the composition and the function of cells in a healthy liver in the context of its spatial organization. Read More

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Facile and rapid detection of SARS-CoV-2 antibody based on a noncompetitive fluorescence polarization immunoassay in human serum samples.

Biosens Bioelectron 2021 Jun 5;190:113414. Epub 2021 Jun 5.

Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan; Innovative Research Centre for Preventive Medical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan; Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan. Electronic address:

Antibody detection methods for viral infections have received broad attention due to the COVID-19 pandemic. In addition, there remains an ever-increasing need to quantitatively evaluate the immune response to develop vaccines and treatments for COVID-19. Here, we report an analytical method for the rapid and quantitative detection of SARS-CoV-2 antibody in human serum by fluorescence polarization immunoassay (FPIA). Read More

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Pitfalls in HLA ligandomics - How to catch a li(e)gand.

Mol Cell Proteomics 2021 Jun 12:100110. Epub 2021 Jun 12.

Immatics Biotechnologies GmbH, Paul-Ehrlich-Str. 15-17, 72076 Tübingen, Germany; Immatics US, Inc. 2201 W. Holcombe Blvd., Suite 205, Houston, Texas 77030, USA. Electronic address:

Knowledge about the peptide repertoire presented by human leukocyte antigens (HLA) holds the key to unlock target-specific cancer immunotherapies like adoptive cell therapies or bispecific T cell engaging receptors. Therefore, comprehensive and accurate characterization of HLA peptidomes by mass spectrometry (immunopeptidomics) across tissues and disease states is essential. With growing numbers of immunopeptidomics datasets and the scope of peptide identification strategies reaching beyond the canonical proteome, the likelihood for erroneous peptide identification as well as false annotation of HLA-independent peptides as HLA ligands is increasing. Read More

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Sensitive and quantitative detection of MHC-I displayed neoepitopes using a semi-automated workflow and TOMAHAQ mass spectrometry.

Mol Cell Proteomics 2021 Jun 12:100108. Epub 2021 Jun 12.

Genentech, South San Francisco, 94080, California, USA. Electronic address:

Advances in several key technologies, including MHC peptidomics, have helped fuel our understanding of basic immune regulatory mechanisms and the identification of T cell receptor targets for the development of immunotherapeutics. Isolating and accurately quantifying MHC-bound peptides from cells and tissues enables characterization of dynamic changes in the ligandome due to cellular perturbations. However, the current multi-step analytical process is challenging, and improvements in throughput and reproducibility would enable rapid characterization of multiple conditions in parallel. Read More

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Microscopic and molecular detection of Deraiophoronema evansi (Lewis, 1882) in domestic Bactrian camels (Camelus bactrianus) of Mongolia.

Parasitol Int 2021 Jun 12:102404. Epub 2021 Jun 12.

Institute of Veterinary Medicine, Mongolian University of Life Science, Zaisan, 17042, Ulaanbaatar, Mongolia. Electronic address:

Cameline filarosis is an important parasitic disease having an economic impact on the camel industry around the world. However, there has been no study on filarosis in Bactrian camels of Mongolia. Therefore, the aim of the present study was to detect and identify microfilariae of Deraiophoronema evansi (D. Read More

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Vascularized Carotid Atherosclerotic Plaque Models for the Validation of Novel Methods of Quantifying Intra-Plaque Neovascularization.

J Am Soc Echocardiogr 2021 Jun 12. Epub 2021 Jun 12.

Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Canada;; Department of Medicine, Cardiovascular Imaging Network at Queen's (CINQ), Queen's University, Canada. Electronic address:

Background: Intraplaque neovascularization (IPN) in advanced lesions of the carotid artery has been linked to plaque progression and risk of rupture. Quantitative measurement of IPN may provide a more powerful tool for the detection of such "vulnerable" plaque than the current visual scoring grading method. The aim of this study was to develop a phantom platform of a neovascularized atherosclerotic plaque within a carotid artery to assess new methods of quantifying IPN. Read More

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A Fluorescence Sensor Array Based on Zinc(II)-carboxyamidoquinolines: Toward Quantitative Detection of ATP.

Chemistry 2021 Jun 15. Epub 2021 Jun 15.

BGSU: Bowling Green State University, Chemistry, UNITED STATES.

The newly prepared fluorescent carboxyamidoquinolines ( 1 - 3 ) and their Zn(II) complexes ( [email protected] - [email protected] ) were used to bind and sense various phosphate anions utilizing a relay mechanism, in which the Zn(II) ion migrates from the [email protected] - [email protected] complexes to the phosphate, namely adenosine 5'-triphosphate (ATP) and pyrophosphate (PPi), a process accompanied by a dramatic change in fluorescence. [email protected] - [email protected] assemblies bind adenine nucleotide phosphates while displaying an analyte-specific response. This process was investigated using UV-vis, fluorescence and NMR spectroscopy. Read More

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CleftNet: Augmented Deep Learning for Synaptic Cleft Detection from Brain Electron Microscopy.

Yi Liu Shuiwang Ji

IEEE Trans Med Imaging 2021 Jun 15;PP. Epub 2021 Jun 15.

Detecting synaptic clefts is a crucial step to investigate the biological function of synapses. The volume electron microscopy (EM) allows the identification of synaptic clefts by photoing EM images with high resolution and fine details. Machine learning approaches have been employed to automatically predict synaptic clefts from EM images. Read More

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Identification of Resistant Germplasm and Detection of Genes for Resistance to Powdery Mildew and Leaf Rust from 2,978 Wheat Accessions.

Plant Dis 2021 Jun 15. Epub 2021 Jun 15.

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Center for Agricultural Resources Research, 286 Huaizhong Rd., Shijiazhuang 050021, China, Shijiazhuang, China, 050021;

Powdery mildew and leaf rust, caused by Blumeria graminis f. sp. tritici (Bgt) and Puccinia triticina (Pt), respectively, are widespread diseases of wheat worldwide. Read More

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First report of infecting white clover () in Finland.

Plant Dis 2021 Jun 15. Epub 2021 Jun 15.

Finnish Food Authority, Laboratory and Research, Mustialankatu 3, Helsinki, Finland, 00790;

Soybean dwarf virus (SbDV, genus ) is a single-stranded positive-sense RNA virus able to infect several legume species. SbDV was first reported in Japan where it was associated with significant yield losses in soybean (Tamada, 1969). Since then the virus has been detected worldwide. Read More

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First Report of Bacterial Rot Caused by Pantoea endophytica on Tobacco in Liuyang, China.

Plant Dis 2021 Jun 15. Epub 2021 Jun 15.

Chinese Academy of Agricultural Sciences Institute of Tobacco Research, 243822, Qingdao, Shandong, China;

Tobacco, Nicotiana tabacum L., is produced largely in China (~1/3 of the global market). In the monsoon summer of 2020, tobacco plant petioles, where axillary buds were removed, became black-rotten, and thick ooze appeared, when squeezed. Read More

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Adaptation of the World Health Organization Electronic Mental Health Gap Action Programme Intervention Guide App for Mobile Devices in Nepal and Nigeria: Protocol for a Feasibility Cluster Randomized Controlled Trial.

JMIR Res Protoc 2021 Jun 15;10(6):e24115. Epub 2021 Jun 15.

Centre for Global Mental Health, Health Service and Population Research Department, Institute of Psychology, Psychiatry & Neuroscience, King's College London, London, United Kingdom.

Background: There is a growing global need for scalable approaches to training and supervising primary care workers (PCWs) to deliver mental health services. Over the past decade, the World Health Organization Mental Health Gap Action Programme Intervention Guide (mhGAP-IG) and associated training and implementation guidance have been disseminated to more than 100 countries. On the basis of the opportunities provided by mobile technology, an updated electronic Mental Health Gap Action Programme Intervention Guide (e-mhGAP-IG) is now being developed along with a clinical dashboard and guidance for the use of mobile technology in supervision. Read More

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Characterization of Compound-Specific Chlorine Isotopologue Distributions of Polychlorinated Organic Compounds by GC-HRMS for Source Identification.

Anal Chem 2021 Jun 15. Epub 2021 Jun 15.

State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.

Distributions of chlorine isotopologues are potentially a fingerprint feature of organochlorines. However, the exact distributions remain little known. This study measured compound-specific chlorine isotopologue distributions of six polychlorinated organic compounds (POCs) for source identification. Read More

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Allergen extract- and component-based diagnostics in children of the ALLIANCE asthma cohort.

Clin Exp Allergy 2021 Jun 15. Epub 2021 Jun 15.

Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Hannover, BREATH German Center for Lung Research (DZL) ), Germany.

Background: Current in vitro allergen-specific IgE (sIgE) detection assays measure IgE against allergen extracts or molecules in a single- or multiplex approach. Direct comparisons of the performance of such assays among young children with common presentations of allergic diseases regardless of sensitization status are largely missing.

Objectives: The aim of this study was a comparison of the analytical and diagnostic performance for common clinical questions of three commonly used technologies which rely upon different laboratory methodologies among children of the All Age Asthma (ALLIANCE) cohort (clinicaltrials. Read More

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Deep Learning in Classification of Covid-19 Coronavirus, Pneumonia and Healthy Lungs on CXR and CT Images.

J Med Biol Eng 2021 Jun 10:1-9. Epub 2021 Jun 10.

Division of Graduate Studies, Tijuana Institute of Technology, Baja CA 22414 Tijuana, Mexico.

Purpose: In this paper, the transfer learning method has been implemented to chest X-ray (CXR) and computed tomography (CT) bio-images of diverse kinds of lungs maladies, including CORONAVIRUS 2019 (COVID-19). COVID-19 identification is a difficult assignment that constantly demands a careful analysis of a patient's clinical images, as COVID-19 is found to be very alike to pneumonic viral lung infection. In this paper, a transfer learning model to accelerate prediction processes and to assist medical professionals is proposed. Read More

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Is it necessary to add the eluate testing to the direct antiglobulin test to improve the detection of maternal erythrocyte alloantibodies?

Transfus Apher Sci 2021 Jun 3:103177. Epub 2021 Jun 3.

University of Antioquia, Medellín, Colombia.

Background: The screening of umbilical cord blood samples by the Direct Antiglobulin Test (DAT) is the reference tool for the identification of maternal erythrocyte alloantibodies present in erythrocytes; however, its diagnostic usefulness is controversial.

Objective: To evaluate the diagnostic validity, safety, and efficiency of the eluate testing (detection of antibody in erythrocyte eluates by the Indirect Antiglobulin Test/IAT) in cord blood samples for detection of maternal erythrocyte alloantibodies in comparison with the DAT.

Materials And Methods: Evaluation study of diagnostic tests. Read More

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Interpreting deep learning models for epileptic seizure detection on EEG signals.

Artif Intell Med 2021 Jul 1;117:102084. Epub 2021 May 1.

Embedded Systems Laboratory (ESL), EPFL, Lausanne, Switzerland.

While Deep Learning (DL) is often considered the state-of-the art for Artificial Intel-ligence-based medical decision support, it remains sparsely implemented in clinical practice and poorly trusted by clinicians due to insufficient interpretability of neural network models. We have approached this issue in the context of online detection of epileptic seizures by developing a DL model from EEG signals, and associating certain properties of the model behavior with the expert medical knowledge. This has conditioned the preparation of the input signals, the network architecture, and the post-processing of the output in line with the domain knowledge. Read More

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Genome-wide detection and analysis of CRISPR-Cas off-targets.

Prog Mol Biol Transl Sci 2021 4;181:31-43. Epub 2021 Mar 4.

Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, MA, United States; Department of Molecular, Cell and Cancer Biology, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, United States. Electronic address:

The clustered, regularly interspersed, short palindromic repeats (CRISPR) technology is revolutionizing biological studies and holds tremendous promise for treating human diseases. However, a significant limitation of this technology is that modifications can occur on off-target sites lacking perfect complementarity to the single guide RNA (sgRNA) or canonical protospacer-adjacent motif (PAM) sequence. Several in vivo and in vitro genome-wide off-target profiling approaches have been developed to inform on the fidelity of gene editing. Read More

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Identification of genomic regions affecting production traits in pigs divergently selected for feed efficiency.

Genet Sel Evol 2021 Jun 14;53(1):49. Epub 2021 Jun 14.

GenPhySE, Université de Toulouse, INRAE, ENVT, 31320, Castanet-Tolosan, France.

Background: Feed efficiency is a major driver of the sustainability of pig production systems. Understanding the biological mechanisms that underlie these agronomic traits is an important issue for environment questions and farms' economy. This study aimed at identifying genomic regions that affect residual feed intake (RFI) and other production traits in two pig lines divergently selected for RFI during nine generations (LRFI, low RFI; HRFI, high RFI). Read More

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Spectral deep learning for prediction and prospective validation of functional groups.

Chem Sci 2020 Mar 13;11(18):4618-4630. Epub 2020 Mar 13.

Department of Chemistry, Purdue University 560 Oval Drive West Lafayette IN 47907 USA

State-of-the-art identification of the functional groups present in an unknown chemical entity requires the expertise of a skilled spectroscopist to analyse and interpret Fourier transform infra-red (FTIR), mass spectroscopy (MS) and/or nuclear magnetic resonance (NMR) data. This process can be time-consuming and error-prone, especially for complex chemical entities that are poorly characterised in the literature, or inefficient to use with synthetic robots producing molecules at an accelerated rate. Herein, we introduce a fast, multi-label deep neural network for accurately identifying all the functional groups of unknown compounds using a combination of FTIR and MS spectra. Read More

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Approach trajectory and solar position affect host plant attractiveness to the small white butterfly.

Vision Res 2021 Jun 11;186:140-149. Epub 2021 Jun 11.

Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.

While it is well documented that insects exploit polarized sky light for navigation, their use of reflected polarized light for object detection has been less well studied. Recently, we have shown that the small white butterfly, Pieris rapae, distinguishes between host and non-host plants based on the degree of linear polarization (DoLP) of light reflected from their leaves. To determine how polarized light cues affect host plant foraging by female P. Read More

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Seasonal distribution of Haemaphysalis longicornis (Acari: Ixodidae) and detection of SFTS virus in Gyeongbuk Province, Republic of Korea, 2018.

Acta Trop 2021 Jun 11:106012. Epub 2021 Jun 11.

Department of Ecological Science, Kyungpook National University, Sangju 37224, South Korea; Department of Vector Entomology, Kyungpook National University, Sangju 37224, Korea. Electronic address:

The seasonal distribution of hard ticks was investigated in 2018 in Gyeongbuk Province, Republic of Korea. Ticks were assayed for severe fever with thrombocytopenia syndrome virus (SFTSV). Ticks were collected monthly using CO-baited traps from April to November in four habitats (grasslands, grave sites, hiking trails, and mixed forests). Read More

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Significance of hepatitis B surface antigen, IgM/IgG core antibody and hepatitis B virus DNA in blood donors.

Gac Med Mex 2021 ;157(1):35-40

Blood Banks Referral Unit, Centro de Investigación para la Salud en América Latina.

Introduction: Identification of hepatitis B virus carriers in blood donors is imperative in order to avoid transmission of the disease via blood transfusion.

Objective: To determine if blood donors with positive results for serological markers HBsAg and anti-HBc were hepatitis B virus DNA carriers.

Methods: 12,745 samples were collected from six Ecuadorian blood banks and analyzed for HBsAg, anti-HBc and anti-HBs infectious markers by automated ELISA. Read More

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January 2021

Phenotypic detection of extended-spectrum beta-lactamase in multidrug-resistant acinetobacter baumannii isolated in Fauji Foundation Hospital Rawalpindi.

J Pak Med Assoc 2021 Apr;71(4):1144-1147

Department of Microbiology, Fauji Foundation Hospital, Rawalpindi, Pakistan.

Objective: To evaluate the phenotypic detection of extended-spectrum betalactamase in multidrug-resistant acinetobacter baumannii.

Methods: The cross-sectional study was conducted at the Department of Microbiology, Fauji Foundation Hospital, Rawalpindi, Pakistan, from August 2018 to April 2019, after the ethical approval from the Institutional Review Committee. Consecutive Non- probability sampling technique was used, and comprised clinical specimens, including pus, blood, sputum, urine, tracheal tubes and canula double lumen, which were processed using standard protocols. Read More

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Simulation of superselective catheterization for cerebrovascular lesions using a virtual injection software.

CVIR Endovasc 2021 Jun 14;4(1):52. Epub 2021 Jun 14.

Department of Neuro-Interventional Surgery, North Shore University Hospital, Northwell Health, 300 Community Drive, Manhasset, NY, 11030, USA.

Background: This report addresses the feasibility of virtual injection software based on contrast-enhanced cone-beam CTs (CBCTs) in the context of cerebrovascular lesion embolization. Intracranial arteriovenous malformation (AVM), dural arteriovenous fistula (AVF) and mycotic aneurysm embolization cases with CBCTs performed between 2013 and 2020 were retrospectively reviewed. Cerebrovascular lesions were reviewed by 2 neurointerventionalists using a dedicated virtual injection software (EmboASSIST, GE Healthcare; Chicago, IL). Read More

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Cerebrospinal fluid lipidomics for biomarkers of Alzheimer's disease.

Mol Omics 2021 Jun;17(3):454-463

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55902, USA. and McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA and Center for Individualized Medicine, Mayo Clinic, Rochester, MN 55905, USA and Department of Biological Chemistry, Pathology and Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.

Alzheimer's disease (AD) is the most common cause of dementia and is associated with serious neurologic sequelae resulting from neurodegenerative changes. Identification of markers of early-stage AD could be important for designing strategies to arrest the progression of the disease. The brain is rich in lipids because they are crucial for signal transduction and anchoring of membrane proteins. Read More

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MASH-FRET: A Simplified Approach for Single-Molecule Multiplexing Using FRET.

Anal Chem 2021 Jun 14. Epub 2021 Jun 14.

Department of Chemistry, Virginia Commonwealth University, Richmond, Virginia 23284, United States.

Multiplexed detection has been a big motivation in biomarker analysis as it not only saves cost and labor but also improves the reliability of diagnosis. Among the many approaches for multiplexed detection, fluorescence resonance energy transfer (FRET)-based multiplexing is gaining popularity particularly due to its low background and quantitative nature. Although several FRET-based approaches have been developed for multiplexing, they require either multiple FRET pairs in combination with multiple excitation sources or complicated algorithms to accurately assign signals for individual FRET pairs. Read More

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Multiplex technologies for the assessment of minimal residual disease and low-level mutation detection in leukaemia: mass spectrometry versus next-generation sequencing.

Br J Haematol 2021 Jun 14. Epub 2021 Jun 14.

Department of Genetics and Molecular Pathology and Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia.

With the focus of leukaemia management shifting to the implications of low-level disease burden, increasing attention is being paid on the development of highly sensitive methodologies required for detection. There are various techniques capable of identification of measurable residual disease (MRD) either evidencing as relevant mutation detection [e.g. Read More

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Visual or visual-tactile examination to detect and inform the diagnosis of enamel caries.

Cochrane Database Syst Rev 2021 Jun 14;6:CD014546. Epub 2021 Jun 14.

Dundee Dental School, University of Dundee, Dundee, UK.

Background: The detection and diagnosis of caries at the initial (non-cavitated) and moderate (enamel) levels of severity is fundamental to achieving and maintaining good oral health and prevention of oral diseases. An increasing array of methods of early caries detection have been proposed that could potentially support traditional methods of detection and diagnosis. Earlier identification of disease could afford patients the opportunity of less invasive treatment with less destruction of tooth tissue, reduce the need for treatment with aerosol-generating procedures, and potentially result in a reduced cost of care to the patient and to healthcare services. Read More

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