980,496 results match your criteria detection


Development of a highly sensitive sensor chip using optical diagnostic based on functionalized plasmonically active AuNPs.

Nanotechnology 2021 May 12. Epub 2021 May 12.

Faculty of Materials Science and Technology, University of Science, Vietnam National University Ho Chi Minh City University of Science, Ho Chi Minh City, VIET NAM.

Measuring solution concentration plays an important role in chemical, biochemical, clinical diagnosis, environmental monitoring, and biological analyses. In this work, we develop a transmission-mode localized surface plasmon resonance (LSPR) sensor chip system and convenient method which is highly efficient, highly sensitive for detection sensing using multimode fiber. The plasmonically active sensor's surface AuNPs with high-density NPs were decorated onto 1 cm sensing length of various clad-free fiber in the form of homogeneous monolayer utilizing a self-assembly process for immobilization of the target molecule. Read More

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Coding of plant immune signals by surface receptors.

Curr Opin Plant Biol 2021 May 9;62:102044. Epub 2021 May 9.

Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, Vienna, Austria. Electronic address:

The detection of molecular signals derived from other organisms is central to the evolutionary success of plants in the colonization of Earth. The sensory coding of these signals is critical for marshaling local and systemic immune responses that keep most invading organisms at bay. Plants detect immune signals inside and outside their cells using receptors. Read More

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Towards improved breast mass detection using dual-view mammogram matching.

Med Image Anal 2021 Apr 22;71:102083. Epub 2021 Apr 22.

Inserm, LaTIM UMR 1101, 22 rue Camille Desmoulins, Brest 29238, France; IMT Atlantique, Technopôle Brest-Iroise, Brest 29238, France.

Breast cancer screening benefits from the visual analysis of multiple views of routine mammograms. As for clinical practice, computer-aided diagnosis (CAD) systems could be enhanced by integrating multi-view information. In this work, we propose a new multi-tasking framework that combines craniocaudal (CC) and mediolateral-oblique (MLO) mammograms for automatic breast mass detection. Read More

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Ultrasensitive determination of serotonin in human urine by a two dimensional capillary isotachophoresis-capillary zone electrophoresis hyphenated with tandem mass spectrometry.

J Chromatogr A 2021 Apr 27;1648:462190. Epub 2021 Apr 27.

Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojarov 10, SK-832 32 Bratislava, Slovak Republic; Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Odbojarov 10, SK-832 32 Bratislava, Slovak Republic. Electronic address:

A two-dimensional capillary isotachophoresis-capillary zone electrophoresis method hyphenated with tandem mass spectrometry was developed and validated for ultrasensitive quantification of serotonin in real human urine samples. Under optimal conditions, the separation was achieved within 12 min (including on-line sample preparation) with the limit of detection of 34 pg mL (due to a large volume sample injection, here 10 µL, and isotachophoretic preconcentration). This concentration limit represents the lowest value for serotonin in comparison to other previously published separation methods employing mass spectrometry detection and applied to urine matrices. Read More

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A novel approach to diagnose sleep apnea using enhanced frequency extraction network.

Comput Methods Programs Biomed 2021 Apr 28;206:106119. Epub 2021 Apr 28.

Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Sleep apnea-hypopnea syndrome (SAHS), as a widespread respiratory sleep disorder, if left untreated, can lead to a series of pathological changes. By using Polysomnography (PSG), traditional SAHS diagnosis tends to be complex and costly. Nasal airflow (NA) is the most direct reflection of the severity of SAHS. Read More

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An innovative and simple all electrochemical approach to functionalize electrodes with a carbon nanotubes/polypyrrole molecularly imprinted nanocomposite and its application for sulfamethoxazole analysis.

J Colloid Interface Sci 2021 Apr 30;599:676-685. Epub 2021 Apr 30.

Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali (Di.S.Te.B.A.), Università del Salento, via Monteroni, 73100 Lecce, Italy.

Sulfamethoxazole (SMX) is a commonly used antibiotic which accumulation can favor the development of antimicrobial resistance. Therefore, easy and cheap system to monitor the presence of SMX are needed for human health protection. Herein we present a straightforward all electrochemical approach to fabricate a sensor based on a nanocomposite molecularly imprinted polymer (nanoMIP) for the determination of SMX. Read More

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Organic matter interference with steroid hormone removal by single-walled carbon nanotubes - ultrafiltration composite membrane.

Water Res 2021 Apr 14;199:117148. Epub 2021 Apr 14.

Institute for Advanced Membrane Technology (IAMT), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. Electronic address:

Composite adsorbent-ultrafiltration membranes for micropollutant removal are an interesting concept, although water matrix compounds, such as organic matter (OM), may interfere with micropollutant adsorption. To prevent this interference, the adsorbents such as single-walled carbon nanotubes (SWCNTs) can be deposited on the permeate side of an ultrafiltration membrane (UF) that can retain OM. In this study, the 'shielding' effect by different UF MWCOs of such an SWCNT-UF composite was evaluated for nine different types of OM. Read More

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Design and development of ferrocene-containing chitosan-cografted-branched polyethylenimine redox conjugates for monitoring free flap failure after reconstructive surgery.

Biosens Bioelectron 2021 Apr 30;186:113283. Epub 2021 Apr 30.

Medical Technology Office, SingHealth, 20 College Road, Academia, 169856, Singapore. Electronic address:

We reported a proof-of-concept study of developing an electrochemical biosensor for prolonged continuous monitoring free flap failure caused by vascular occlusion after reconstructive surgery. Ferrocene (Fc)-containing Chitosan-cografted-Branched Polyethylenimine redox conjugates (CHIT-Fc-co-BPEI-Fc) were used as pH-tuneable matrix to attach the target enzymes (glucose oxidase ≡ GOD and lactate oxidase ≡ LOD, respectively) to build up the corresponding GOD-sensor and LOD-sensor. The sensitivity of GOD-/LOD-sensor was found to be 2. Read More

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Aptamer-based lateral flow assay on-site biosensors.

Biosens Bioelectron 2021 Apr 24;186:113279. Epub 2021 Apr 24.

School of Information and Communication Engineering, University of Electronic Science and Technology of China, Chengdu, 611731, China. Electronic address:

The lateral flow assay (LFA) is a widely used paper-based on-site biosensor that can detect target analytes and obtain test results in several minutes. Generally, antibodies are utilized as the biorecognition molecules in the LFA. However, antibodies selected using an in vivo process not only may risk killing the animal hosts and causing errors between different batches but also their range is restricted by the refrigerated conditions used to store them. Read More

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Aberrant expression of miR-148a-3p in Alzheimer's disease and its protective role against amyloid-β induced neurotoxicity.

Neurosci Lett 2021 May 9:135953. Epub 2021 May 9.

Department of Neurology, Shanxian Central Hospital, Heze 274300, Shandong, China. Electronic address:

Objective: The current study investigated the expression change and clinical value of miR-148a-3p in AD patients, and further examined the role of miR-148a-3p in Aβ-induced neurotoxicity in SH-SY5Y cells.

Material And Methods: qRT-PCR was used for the measurement of miR-148a-3p expression levels. ROC curve was established to calculate the diagnostic value of serum miR-148a-3p for AD. Read More

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High Throughput Quantification of Short Nucleic Acid Samples by Capillary Electrophoresis with Automated Data Processing.

Anal Biochem 2021 May 9:114239. Epub 2021 May 9.

Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, 2500 Speedway, Austin, TX, 78712, USA. Electronic address:

Analysis of catalytic activity of nucleic acid enzymes is crucial for many applications, ranging from biotechnology to the search for antiviral drugs. Commonly used analytical methods for quantifying DNA and RNA reaction products based on slab-gel electrophoresis are limited in throughput, speed, and accuracy. Here we report the optimization of high throughput methods to separate and quantify short nucleic acid reaction products using DNA sequencing instruments based on capillary electrophoresis with fluorescence detection. Read More

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Development of a novel multiplex PCR assay for the detection and differentiation of Plasmodium caprae from Theileria luwenshuni and Babesia spp. in goats.

Acta Trop 2021 May 9:105957. Epub 2021 May 9.

Veterinary Parasitology Research Unit, Department of Pathology Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address:

Intraerythrocytic parasites are traditionally identified by the microscopic examination of Giemsa-stained blood smears. However, this method does not always allow for the identification of individual species in goat's RBCs. Moreover, its unreliability in detecting low levels of parasitemia makes it unsuitable for epidemiological investigations and leaves goat farms vulnerable to potential outbreaks. Read More

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Clinical Chemistry for Developing Countries: Mass Spectrometry.

Annu Rev Anal Chem (Palo Alto Calif) 2021 May 12. Epub 2021 May 12.

Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA; email:

Early disease diagnosis is necessary to enable timely interventions. Implementation of this vital task in the developing world is challenging owing to limited resources. Diagnostic approaches developed for resource-limited settings have often involved colorimetric tests (based on immunoassays) due to their low cost. Read More

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High-Throughput Screening of an Octanoic Acid Producer Strain Library Enables Detection of New Targets for Increasing Titers in .

ACS Synth Biol 2021 May 12. Epub 2021 May 12.

Institute of Molecular Biosciences, Faculty of Biological Sciences, Goethe University Frankfurt, Max-von-Laue Straße 9, 60438 Frankfurt am Main, Germany.

Octanoic acid is an industrially relevant compound with applications in antimicrobials or as a precursor for biofuels. Microbial biosynthesis through yeast is a promising alternative to current unsustainable production methods. To increase octanoic acid titers in , we use a previously developed biosensor that is based on the octanoic acid responsive promotor coupled to GFP. Read More

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Quantitative proteomics analysis identifies salivary biomarkers for early detection of pregnancy loss in a Singaporean cohort - A pilot study.

Proteomics Clin Appl 2021 May 12:e2000068. Epub 2021 May 12.

Associate Professor, Singapore Oral Microbiomics Initiative, National Dental Research Institute Singapore, National Dental Center Singapore, Oral Health Academic Clinical Program, Duke NUS Medical School, Singapore.

Purpose: Early pregnancy loss (EPL) is one of the most common complications encountered in clinical practice. As most of EPLs occur relatively early on during pregnancy, they are often misunderstood as an expected menstrual cycle. Thus, it is essential to investigate the diagnostic biomarkers for monitoring pregnancy loss for continuous non-invasive monitoring of EPL. Read More

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Direct Immunodetection of Global A-to-I RNA Editing Activity with a Chemiluminescent Bioassay.

Angew Chem Int Ed Engl 2021 May 12. Epub 2021 May 12.

Emory University, Department of Chemistry, 1515 Dickey Dr., 30322, Atlanta, UNITED STATES.

Adenosine-to-inosine (A-to-I) editing is a conserved RNA modification that contributes to immune response and overall cellular function. RNA editing patterns can vary significantly between different cell and tissue types, and hyperactive A-to-I signatures are indicative of several diseases, including cancer and autoimmune disorders. Because of the importance of these differences, there is significant need for efficient methods to measure overall A-to-I editing levels in cellular RNA. Read More

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Blood-based biomarkers and long-term risk of frailty - experience from the Swedish AMORIS cohort.

J Gerontol A Biol Sci Med Sci 2021 May 12. Epub 2021 May 12.

Unit of Epidemiology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

Background: Frailty is associated with reduced quality of life, poor health outcomes, and death. Past studies have investigated how specific biomarkers are associated with frailty but understanding biomarkers in concert with each other and the associated risk of frailty is critical for clinical application.

Methods: Using a sample aged ≥59 years at baseline from the Swedish AMORIS cohort (n=19341), with biomarkers measured at baseline (1985-1996), we conducted latent class analysis with 18 biomarkers and used Cox models to determine the association between class and frailty and all-cause mortality. Read More

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Detection of mind wandering using EEG: Within and across individuals.

PLoS One 2021 12;16(5):e0251490. Epub 2021 May 12.

Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California, United States of America.

Mind wandering is often characterized by attention oriented away from an external task towards our internal, self-generated thoughts. This universal phenomenon has been linked to numerous disruptive functional outcomes, including performance errors and negative affect. Despite its prevalence and impact, studies to date have yet to identify robust behavioral signatures, making unobtrusive, yet reliable detection of mind wandering a difficult but important task for future applications. Read More

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A fast and effective detection framework for whole-slide histopathology image analysis.

PLoS One 2021 12;16(5):e0251521. Epub 2021 May 12.

Department of Pathology, Huazhong University of Science and Technology, Tongji Medical College, Hubei Cancer Hospital, Wuhan, China.

Pathologists generally pan, focus, zoom and scan tissue biopsies either under microscopes or on digital images for diagnosis. With the rapid development of whole-slide digital scanners for histopathology, computer-assisted digital pathology image analysis has attracted increasing clinical attention. Thus, the working style of pathologists is also beginning to change. Read More

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Performance of the Abbott RealTime MTB and RIF/INH resistance assays for the detection of Mycobacterium Tuberculosis and resistance markers in sputum specimens.

PLoS One 2021 12;16(5):e0251602. Epub 2021 May 12.

Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.

Background: The Abbott RealTime MTB is an assay for the detection of Mycobacterium tuberculosis (MTB) complex DNA from respiratory specimens in combination with the Abbott RealTime RIF/INH assay for the detection of genetic resistance markers for isoniazid (INH) and rifampicin (RIF) from MTB positive isolates. Hence, this study aimed to evaluate the performance of the Abbott RealTime MTB and RIF/INH assays.

Methods: A cross-sectional study was conducted on 289 study subjects presumptive to have pulmonary tuberculosis at Nigist Eleni Mohammed Memorial Hospital, South Ethiopia from April 2017 to June 2018. Read More

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The influence on oxidative stress markers, inflammatory factors and intestinal injury-related molecules in Wahui pigeon induced by lipopolysaccharide.

PLoS One 2021 12;16(5):e0251462. Epub 2021 May 12.

College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.

Introduction: The intestinal structure is the foundation for various activities and functions in poultry. An important question concerns the changes in the intestinal status under endotoxin stimulation. This study aimed to investigate the mechanism of intestinal injury induced by lipopolysaccharide (LPS) in Wahui pigeons. Read More

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Establishment of a Sandwich-ELISA for simultaneous quantification of bovine pregnancy-associated glycoprotein in serum and milk.

PLoS One 2021 12;16(5):e0251414. Epub 2021 May 12.

Department of Animal Sciences, Georg-August-University, Goettingen, Lower Saxony, Germany.

Bovine pregnancy-associated glycoproteins (boPAG) are expressed by trophoblast cells in the bovine placenta. The multigene family of boPAG belongs to the group of aspartic proteases. The accumulation and circulation in maternal blood and milk has made boPAG very useful and important for pregnancy diagnosis in cattle. Read More

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EMDS-5: Environmental Microorganism image dataset Fifth Version for multiple image analysis tasks.

PLoS One 2021 12;16(5):e0250631. Epub 2021 May 12.

School of Control Engineering, Chengdu University of Information Technology, Chengdu, China.

Environmental Microorganism Data Set Fifth Version (EMDS-5) is a microscopic image dataset including original Environmental Microorganism (EM) images and two sets of Ground Truth (GT) images. The GT image sets include a single-object GT image set and a multi-object GT image set. EMDS-5 has 21 types of EMs, each of which contains 20 original EM images, 20 single-object GT images and 20 multi-object GT images. Read More

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Training and usage of detection dogs to better understand bumble bee nesting habitat: Challenges and opportunities.

PLoS One 2021 12;16(5):e0249248. Epub 2021 May 12.

Faculty of Environmental and Urban Change, York University, Toronto, Ontario, Canada.

Bumble bees are among the most imperiled pollinators. However, habitat use, especially nest site selection, remains relatively unknown. Methods to locate nests are invaluable to better understand habitat requirements and monitor wild populations. Read More

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The IL-10GFP (VeRT-X) mouse strain is not suitable for the detection of IL-10 production by granulocytes during lung inflammation.

PLoS One 2021 12;16(5):e0247895. Epub 2021 May 12.

Izmir Biomedicine and Genome Center (IBG), Balcova/Izmir, Turkey.

The clear and unequivocal identification of immune effector functions is essential to understand immune responses. The cytokine IL-10 is a critical immune regulator and was shown, for example, to limit pathology during various lung diseases. However, the clear identification of IL-10-producing cells is challenging and, therefore, reporter mouse lines were developed to facilitate their detection. Read More

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KerNet: A Novel Deep Learning Approach for Keratoconus and Sub-clinical Keratoconus Detection Based on Raw Data of the Pentacam System.

IEEE J Biomed Health Inform 2021 May 12;PP. Epub 2021 May 12.

Keratoconus is one of the most severe corneal diseases, which is difficult to detect at the early stage (i.e., sub-clinical keratoconus) and possibly results in vision loss. Read More

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Cross-Subject Zero Calibration Driver's Drowsiness Detection: Exploring Spatiotemporal Image Encoding of EEG Signals For Convolutional Neural Network Classification.

IEEE Trans Neural Syst Rehabil Eng 2021 May 12;PP. Epub 2021 May 12.

This paper explores two methodologies for drowsiness detection using EEG signals in a sustained-attention driving task considering pre-event time windows, and focusing on cross-subject zero calibration. Driving accidents are a major cause of injuries and deaths on the road. A considerable portion of those are due to fatigue and drowsiness. Read More

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Efficient Center Voting for Object Detection and 6D Pose Estimation in 3D Point Cloud.

IEEE Trans Image Process 2021 May 12;PP. Epub 2021 May 12.

We present a novel and efficient approach to estimate 6D object poses of known objects in complex scenes represented by point clouds. Our approach is based on the well-known point pair feature (PPF) matching, which utilizes self-similar point pairs to compute potential matches and thereby cast votes for the object pose by a voting scheme. The main contribution of this paper is to present an improved PPF-based recognition framework, especially a new center voting strategy based on the relative geometric relationship between the object center and point pair features. Read More

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Are Korotkoff sounds reliable markers for accurate estimation of systolic and diastolic pressure using brachial cuff sphygmomanometry.

IEEE Trans Biomed Eng 2021 May 12;PP. Epub 2021 May 12.

The accurate measurement of non-invasive blood pressure (NIBP) is a fundamental public health requirement, yet we are aware that NIBP measurements significantly underestimate true systolic blood pressure (SBP) and overestimate diastolic blood pressure (DBP). The aetiology for these errors has not yet been fully established. This study aimed to investigate the accuracy of Korotkoff sounds for accurate detection of SBP and DBP as used in brachial cuff sphygmomanometry. Read More

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