5,607 results match your criteria detected quantities

Spectrally Silent Protein Reaction Dynamics Revealed by Time-Resolved Thermodynamics and Diffusion Techniques.

Acc Chem Res 2021 Apr 22. Epub 2021 Apr 22.

Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, 606-8502 Japan.

ConspectusBiological functions essentially consist of a series of chemical reactions, including intermolecular interactions, and also involve the cooperation of a number of biological molecules performing these reactions. To understand this function at the molecular level, all steps of the reactions must be elucidated. However, since the biosystems including the surrounding environment are notably large, the reactions have to be elucidated from several different approaches. Read More

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Sensitive screening of synthetic cannabinoids using liquid chromatography quadrupole time-of-flight mass spectrometry after solid phase extraction.

Drug Test Anal 2021 Apr 22. Epub 2021 Apr 22.

Institute of Forensic Medicine, Forensic Toxicology, Johannes Gutenberg University Mainz, Mainz, Germany.

Analysis of synthetic cannabinoids still pose a challenge for many institutions due to the number of available substances and the constantly changing drug market. Both new and well-known substances keep appearing and disappearing on the market, making it hard to adapt analytical methods in a timely manner. In this study, we developed a qualitative screening approach for synthetic cannabinoids and their metabolites by means of liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Read More

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[Multidisciplinary management of voluntary pink oleander poisoning: how to estimate the quantities ingested?]

Ann Pharm Fr 2021 Apr 17. Epub 2021 Apr 17.

Service de Réanimation- Soins Continus, Centre Hospitalier de Valence, France. Electronic address:

This is a case of voluntary ingestion of Nerium oleander leaves in an adolescent requiring the use of atropine and emergency chartering of antidigoxin antibodies (Digifab®) due to the difficulty of assessing oleandrin level and associated toxicity. Upon hospital admission, a digoxinemia was performed (0.44 µg/mL) and the presence of oleandrine was detected. Read More

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Uptake of NO by mycorrhizal and non-mycorrhizal Scots pine seedlings: quantities and effects on amino acid and protein concentrations.

New Phytol 1991 Sep;119(1):83-92

Department of Forest Genetics and Plant Physiology.

Scots pine seedlings were grown from sterilized seeds in oven sterilized sand. Half of the seedlings were inoculated with the mycorrhizal fungus Pisolithus arhizus (Pers.) Rausch (syn. Read More

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September 1991

A study of ageing of spruce [Picea sitchensis (Bong.) Carr.] ectomycorrhizas. II. Carbohydrate allocation in ageing Picea sitchensis/Tylospora fibrillosa (Burt.) Donk ectomycorrhizas.

New Phytol 1992 Sep;122(1):153-158

Department of Plant and Soil Science, University of Aberdeen, Aberdeen AB9 2UE, Scotland, UK.

Translocation of C-labelled photosynthate was studied in seedlings of Picea sitchensis (Bong.) Carr. ectomycorrhizal with Tylospora fibrillosa (Burt. Read More

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September 1992

Intraclonal translocation of ammonium and nitrate nitrogen in Carex bigelowii Torr. ex Schwein. using N and nitrate reductase assays.

New Phytol 1990 Mar;114(3):419-428

The Institute of Terrestrial Ecology, Merlewood Research Station, Grange-over-Sands, Cumbria LA11 6JU, UK.

Carex bigelowii Torr. ex Schwein. forms extensive rhizome systems consisting of numerous long-lived tiller generations with only the roots and the rhizome remaining alive, and only few tillers with above-ground shoots. Read More

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Phenols and bud dormancy I. Variations in total phenols and phenylalanine ammonia lyase activity in dormant buds of Fagus sylvatica L.

New Phytol 1988 Jan;108(1):83-89

Dipartimento di Biologia Vegetate, Università di Torino, Viale P.A. Mattioli, 25 I-10125 Torino, Italy.

Quantitative analyses of the total phenols (glucosides and aglycones and phenylalanine ammonia lyase (PAL) activity were determined separately in bud scales and the leaf primordia of dormant buds (apical, subapical and basal) of Fagus sylvatica L, until bud burst. In all the leaf primordia, the phenol and glucoside contents did not change substantially from November to March. From dormancy break to bud burst, phenols and glucosides accumulated in the subapical and basal buds. Read More

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January 1988

Downregulation of TGR5 (GPBAR1) in biliary epithelial cells contributes to the pathogenesis of sclerosing cholangitis.

J Hepatol 2021 Apr 16. Epub 2021 Apr 16.

Department of Gastroenterology, Hepatology and Infectious Diseases, University Hospital Düsseldorf, Medical Faculty of Heinrich Heine University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address:

Background&aims: Primary sclerosing cholangitis (PSC) is characterized by chronic inflammation and progressive fibrosis of the biliary tree. The bile acid receptor TGR5 is found on biliary epithelial cells (BECs), where it promotes secretion, proliferation and tight junction integrity. Thus, we speculated that changes in TGR5-expression in BECs may contribute to PSC pathogenesis. Read More

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Cross-Species Metabolic Profiling of Floral Specialized Metabolism Facilitates Understanding of Evolutional Aspects of Metabolism Among Brassicaceae Species.

Front Plant Sci 2021 31;12:640141. Epub 2021 Mar 31.

Graduate School of Biological Science, Nara Institute of Science and Technology (NAIST), Ikoma, Japan.

Plants produce a variety of floral specialized (secondary) metabolites with roles in several physiological functions, including light-protection, attraction of pollinators, and protection against herbivores. Pigments and volatiles synthesized in the petal have been focused on and characterized as major chemical factors influencing pollination. Recent advances in plant metabolomics have revealed that the major floral specialized metabolites found in land plant species are hydroxycinnamates, phenolamides, and flavonoids albeit these are present in various quantities and encompass diverse chemical structures in different species. Read More

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Cottonseed Press Cake as a Potential Diet for Industrially Farmed Black Soldier Fly Larvae Triggers Adaptations of Their Bacterial and Fungal Gut Microbiota.

Front Microbiol 2021 29;12:634503. Epub 2021 Mar 29.

Department of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany.

Black soldier fly larvae (, Diptera: Stratiomyidae) are used for the bioconversion of organic side products into valuable compounds such as proteins, lipids and chitin. However, the economic competitiveness of farmed insects compared to conventional protein production systems in agriculture and aquaculture depends on the availability of large quantities of inexpensive insect feed. Cottonseed press cake (CPC) is a side-stream of cotton production that is rich in proteins and lipids but unsuitable as feed for several farmed animals, except ruminants, due to the presence of the anti-nutritional sesquiterpenoid gossypol. Read More

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Comparative chemical examination of inclusion complexes formed with β-cyclodextrin derivatives and basic amino acids.

Carbohydr Polym 2021 Jun 26;262:117868. Epub 2021 Feb 26.

Department of Chemistry, University of Isfahan, 8174673441, Iran; California Institute for Quantitative Biosciences, University of California, Berkeley, California, USA. Electronic address:

In this study, we have investigated the host-guest inclusion complexes between β-cyclodextrin (βCD), 2-hydroxypropyl-β-cyclodextrin (2-HPβCD), and mono-6-tosyl-β-cyclodextrin (TS-βCD) excipients and two amino acids, such as L-arginine (L-Arg) and L-lysine (L-Lys). The formation of inclusion complexes was detected, and a comparative study was conducted at different pH, density, and viscosity. A physical mixture, comprising equal amount of amino acids was used to prepare the complex in a solid-state form. Read More

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Bedbug salivation patterns during hematophagy in the skin of a mammalian host.

J Insect Physiol 2021 Apr 6;131:104235. Epub 2021 Apr 6.

Laboratório de Fisiologia de Insetos Hematófagos, Departamento de Parasitologia/ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address:

Cimex lectularius (Hemiptera:Cimicidae) infestations have increased over the past decades in several parts of the world, constituting a major urban pest with no reversion signs. The impact on human health caused by these insects, commonly known as bedbugs, is associated with their obligatory hematophagous habit. Allergies induced by hematophagous arthropod bites are related to the deposition of salivary molecules in the host tissues. Read More

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Snapshot of narcotic drugs and psychoactive substances in Kuwait: analysis of illicit drugs use in Kuwait from 2015 to 2018.

BMC Public Health 2021 Apr 7;21(1):671. Epub 2021 Apr 7.

Narcotic and Psychotropic Laboratory, General Department of Criminal Evidence, Ministry of Interior, Al-Dhajeej, Block 1, Al-Farwaniya City, Kuwait.

Background: The misuse of illicit substances is associated with increased morbidity and mortality; thus, substance abuse is a global health concern. The Arabian Gulf region is considered a crossing point and a consumer of illicit drugs. However, a lack of laboratory-based research has limited the scientific assessment of drug misuse in the Arabian Gulf region. Read More

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Occurrence of per- and polyfluoroalkyl substances and unidentified organofluorine in leachate from waste-to-energy stockpile - A case study.

Chemosphere 2021 Mar 29;278:130380. Epub 2021 Mar 29.

Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden.

Per- and polyfluoroalkyl substances (PFASs) are a diverse group of chemicals used in consumer products, which will inevitably end up in waste streams. Landfills are widely recognized secondary point sources of PFASs, but other types of waste management sites have received less attention. Therefore, in a case study presented here we investigated releases of PFASs from temporarily stored waste by determining quantities of 34 PFASs in leachate from a Waste-to-Energy stockpile (45 000 ± 2000 tonnes) during five months in 2019. Read More

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A dynamic model for the COVID-19 with direct and indirect transmission pathways.

Math Methods Appl Sci 2021 Feb 17. Epub 2021 Feb 17.

Department of Mathematics Semnan University Semnan Iran.

Two common transmission pathways for the spread of COVID-19 virus are direct and indirect. The direct pathway refers to the person-to-person transmission between susceptibles and infectious individuals. Infected individuals shed virus on the objects, and new infections arise through touching a contaminated surface; this refers to the indirect transmission pathway. Read More

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February 2021

Double-Imaging Photoelectron Photoion Coincidence Spectroscopy Reveals the Unimolecular Thermal Decomposition Mechanism of Dimethyl Carbonate.

J Phys Chem A 2021 Apr 2;125(14):2895-2904. Epub 2021 Apr 2.

Paul Scherrer Institute, Villigen 5232, Switzerland.

We studied the thermal decomposition of dimethyl carbonate (DMC, CHO) in a flash vacuum pyrolysis reactor in the 1100-1700 K range. The reaction products and intermediates were probed by vacuum ultraviolet synchrotron radiation in a photoelectron photoion coincidence (PEPICO) spectrometer to record isomer-specific photoion mass-selected threshold photoelectron (ms-TPE) spectra. Reaction pathways were explored using quantum chemical calculations, which confirmed the experimental observation that the intramolecular migration of a methyl group, yielding dimethyl ether (DME, CHO) and carbon dioxide, dominates the initial unimolecular decomposition chemistry. Read More

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Factors Associated With MALDI-TOF Mass Spectral Quality of Species Identification in Clinical Routine Diagnostics.

Front Cell Infect Microbiol 2021 16;11:646648. Epub 2021 Mar 16.

Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.

Background: An accurate and timely identification of bacterial species is critical in clinical diagnostics. Species identification allows a potential first adaptation of empiric antibiotic treatments before the resistance profile is available. Matrix assisted Laser Desorption Ionization Time of Flight mass spectrometry (MALDI-TOF MS) is a widely used method for bacterial species identification. Read More

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Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA.

Biophys J 2021 Mar 29. Epub 2021 Mar 29.

Department of Biochemistry and Biophysics Corvallis, Oregon. Electronic address:

The nucleocapsid phosphoprotein N plays critical roles in multiple processes of the severe acute respiratory syndrome coronavirus 2 infection cycle: it protects and packages viral RNA in N assembly, interacts with the inner domain of spike protein, binds to structural membrane (M) protein during virion packaging and maturation, and to proteases causing replication of infective virus particle. Even with its importance, very limited biophysical studies are available on the N protein because of its high level of disorder, high propensity for aggregation, and high susceptibility for autoproteolysis. Here, we successfully prepare the N protein and a 1000-nucleotide fragment of viral RNA in large quantities and purity suitable for biophysical studies. Read More

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Effects of operating parameters on co-gasification of coconut petioles and refuse-derived fuel.

Waste Manag Res 2021 Mar 29:734242X211003974. Epub 2021 Mar 29.

The Joint Graduate School of Energy and Environment, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

Coconut agro-industry in the western region of Thailand generates a large amount of residues. This study investigated the energy production potential of discarded coconut petioles, with a focus on co-gasification with refuse-derived fuel (RDF). Gasification tests involving petioles, RDFs and their mixtures (25%, 50%, 75% or 100% by weight) were conducted in a laboratory-scale fixed bed reactor. Read More

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-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs.

Front Vet Sci 2021 12;8:581148. Epub 2021 Mar 12.

Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil.

Lipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which allows their use in point-of-care testing without special storage conditions. Read More

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Real-time location algorithms of drinking water pollution sources based on domain knowledge.

Environ Sci Pollut Res Int 2021 Mar 27. Epub 2021 Mar 27.

School of Computer Science, China University of Geosciences, Wuhan, 430074, China.

The real-time location of pollution sources is the process of inverting pollution sources based on the dynamic optimization model constructed by the time-varying pollution concentration detected by the water quality sensor. Due to the vast quantities of the water supply networks, the water quality sensors will only be placed on critical nodes, resulting in multiple solutions. However, the increased monitoring data enhances the uniqueness of the solution. Read More

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The fate of plastic litter within estuarine compartments: An overview of current knowledge for the transboundary issue to guide future assessments.

Environ Pollut 2021 Mar 15;279:116908. Epub 2021 Mar 15.

Laboratório de Microcontaminantes Orgânicos e Ecotoxicologia Aquática - Instituto de Oceanografia - Universidade Federal do Rio Grande (FURG). Av. Itália, Km 8, Carreiros, CEP: 96203-900, Rio Grande, RS, Brazil.

Plastics can enter biogeochemical cycles and thus be found in most ecosystems. Most studies emphasize plastic pollution in oceanic ecosystems even though rivers and estuaries are acknowledged as the main sources of plastics to the oceans. This review detected few studies approaching the transboundary issue, as well as patterns of estuarine gradients in predicting plastic distribution and accumulation in water, sediments, and organisms. Read More

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Multiplex Real-Time PCR Method for Simultaneous Detection and Differentiation of Goat Pox Virus, Sheeppox Virus, and Lumpy Skin Disease Virus.

J AOAC Int 2021 Mar 26. Epub 2021 Mar 26.

Chinese Academy of Inspection and Quarantine, The Institute of Animal Quarantine, No. 11, Ronghuananlu Street, Yizhuang Economic and Technological Development Zone, Daxing District, Beijing, China, 100176, People's Republic of China.

Background: The diseases caused by the Capripoxvirus species have very similar symptoms and are difficult to distinguish clinically. According to a recent report, Capripoxvirus are not strictly host specific.

Objective: This study aimed to identify the viruses from ovine (include sheep and goat) or bovine, which will benefit to select the appropriate vaccine and take correct measures to control diseases. Read More

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Nine prohibited stimulants found in sports and weight loss supplements: deterenol, phenpromethamine (Vonedrine), oxilofrine, octodrine, beta-methylphenylethylamine (BMPEA), 1,3-dimethylamylamine (1,3-DMAA), 1,4-dimethylamylamine (1,4-DMAA), 1,3-dimethylbutylamine (1,3-DMBA) and higenamine.

Clin Toxicol (Phila) 2021 Mar 23:1-7. Epub 2021 Mar 23.

Health Protection Center, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.

Background: Weight loss and sports supplements containing deterenol have been associated with serious adverse events including cardiac arrest.

Objective: To determine the presence and quantity of experimental stimulants in dietary supplements labeled as containing deterenol sold in the United States.

Methods: Dietary supplements available for sale in the US and labeled as containing deterenol or one of its synonyms (e. Read More

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Data standardization implementation and applications within and among diagnostic laboratories: integrating and monitoring enteric coronaviruses.

J Vet Diagn Invest 2021 Mar 19:10406387211002163. Epub 2021 Mar 19.

Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA.

Every day, thousands of samples from diverse populations of animals are submitted to veterinary diagnostic laboratories (VDLs) for testing. Each VDL has its own laboratory information management system (LIMS), with processes and procedures to capture submission information, perform laboratory tests, define the boundaries of test results (i.e. Read More

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The relation between ApoB/ApoA-1 ratio and the severity of coronary artery disease in patients with acute coronary syndrome.

Egypt Heart J 2021 Mar 16;73(1):24. Epub 2021 Mar 16.

Faculty of Medicine, Menoufia University, Abdelrahman Elsharkawey St., Shebin Elkom, Menoufia, Egypt.

Background: Apolipoprotein B is considered the primary protein constituent of low-density lipoprotein. LDL contains variable quantities of cholesterol, but each lipoprotein contains a single ApoB protein. Thus, ApoB is a better index for the LDL circulation if compared to LDL cholesterol. Read More

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Prospective association between dietary pesticide exposure profiles and postmenopausal breast-cancer risk in the NutriNet-Santé cohort.

Int J Epidemiol 2021 Mar 15. Epub 2021 Mar 15.

Sorbonne Paris Nord University, INSERM, INRAE, CNAM, Nutritional Epidemiology Research Team (EREN), Epidemiology and Statistics Research Center-University of Paris (CRESS), 93017, Bobigny, France.

Background: Some pesticides, used in large quantities in current agricultural practices all over Europe, are suspected of adverse effects on human reproductive health (breast and prostate cancers), through mechanisms of endocrine disruption and possible carcinogenic properties, as observed in agricultural settings. However, evidence on dietary pesticide exposure and breast cancer (BC) is lacking for the general population. We aimed to assess the associations between dietary exposure to pesticides and BC risk among postmenopausal women of the NutriNet-Santé cohort. Read More

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Persistent organic and inorganic pollutants in the effluents from the textile dyeing industries: Ecotoxicology appraisal via a battery of biotests.

Environ Res 2021 Mar 4;196:110956. Epub 2021 Mar 4.

Department of Chemical Engineering, Faculty of Sciences, University of Granada, Spain. Electronic address:

Today, the textile industry is considered as a leading economic sector in Tunisia. However, this sector demands a huge volume of water and a wide spectrum of chemicals, which is converted into potentially toxic wastewater leading to environmental perturbation and human health toxicity. Assessment of the environmental risks associated with textile wastewater becomes a necessity. Read More

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Oxidative stress- and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective.

Chemosphere 2021 Feb 15;275:129969. Epub 2021 Feb 15.

Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, 100069, China.

Quantities of researches have demonstrated silica nanoparticles (SiNPs) exposure inevitably induced damage to respiratory system, nonetheless, knowledge of its toxicological behavior and metabolic interactions with the cellular machinery that determines the potentially deleterious outcomes are limited and poorly elucidated. Here, the metabolic responses of lung bronchial epithelial cells (BEAS-2B) under SiNPs exposure were investigated using ultra performance liquid chromatography-mass spectrum (UPLC-MS)-based metabolomics research. Results revealed that even with low cytotoxicity, SiNPs disturbed global metabolism. Read More

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February 2021

Multinational evaluation of the BioFire® FilmArray® Pneumonia plus Panel as compared to standard of care testing.

Eur J Clin Microbiol Infect Dis 2021 Mar 2. Epub 2021 Mar 2.

BioFire Diagnostics, LLC, 515 Colorow Way, Salt Lake City, UT, 84108, USA.

This study compared standard of care testing (SOC) to BioFire® FilmArray® Pneumonia plus Panel (PNplus). PNplus detects 15 bacteria with semiquantitative log bin values, 7 antibiotic resistance markers, three atypical bacteria (AB), and eight viral classes directly from bronchoalveolar lavage-like specimens (BLS) and sputum-like specimens (SLS). Fifty-two laboratories from 13 European countries and Israel tested 1234 BLS and 1242 SLS with PNplus and SOC. Read More

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