10,672 results match your criteria detected lipopolysaccharide

Long non-coding RNA CDKN2B-AS1 enhances LPS-induced apoptotic and inflammatory damages in human lung epithelial cells via regulating the miR-140-5p/TGFBR2/Smad3 signal network.

BMC Pulm Med 2021 Jun 14;21(1):200. Epub 2021 Jun 14.

Department of Intensive Care Unit, The Second Clinical Medical College, Jingzhou Central Hospital, Yangtze University, No. 1 Renmin Road, Jingzhou District, Jingzhou, 434000, Hubei, China.

Background: Sepsis is a complicated disease with systemic inflammation or organ dysfunction, and it is the leading cause of acute lung injury (ALI). Long non-coding RNAs (lncRNAs) have played important roles in the pathogenesis of sepsis. This study was designed to explore the biological function and regulatory mechanism of cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1) in lipopolysaccharide (LPS)-induced lung injury. Read More

View Article and Full-Text PDF

lncRNA myocardial infarction-associated transcript (MIAT) knockdown alleviates LPS-induced chondrocytes inflammatory injury via regulating miR-488-3p/sex determining region Y-related HMG-box 11 (SOX11) axis.

Open Life Sci 2021 31;16(1):511-522. Epub 2021 May 31.

Department of Orthopaedic, Taizhou Municipal Hospital, No. 381, Zhongshan East Road, Jiaojiang District, Taizhou 318000, Zhejiang, China.

Long noncoding RNA (lncRNA) has been shown to be involved in the development of osteoarthritis (OA), an age-related bone and joint disease. However, the function and possible molecular mechanism of lncRNA myocardial infarction-associated transcript (MIAT) in lipopolysaccharide (LPS)-induced chondrocytes injury model remain unexplored. Cell viability and apoptosis were detected by methyl thiazolyl tetrazolium (MTT) and flow cytometry, respectively. Read More

View Article and Full-Text PDF

Effect of lactoferrin on murine sperm apoptosis induced by intraperitoneal injection of lipopolysaccharide.

J Vet Med Sci 2021 Jun 11. Epub 2021 Jun 11.

Department of Laboratory Animal Science, Faculty of Agriculture, Tottori University.

Genital bacterial infection is one of the most important causes of infertility, however, bacteria frequently exist in seminal fluid. Sperm express Toll-like receptors (TLRs) on their cell surfaces and bacterial recognition by TLRs induces sperm apoptosis. In this study, we examined the lactoferrin (LF) potentiality on sperm apoptosis induced by bacterial lipopolysaccharide (LPS). Read More

View Article and Full-Text PDF

Lipopolysaccharide-induced depression is associated with estrogen receptor-α/SIRT1/NF-κB signaling pathway in old female mice.

Neurochem Int 2021 Jun 10:105097. Epub 2021 Jun 10.

Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, China.

The present study aims to investigate the influence of sex/age on depressive-like behaviors in lipopolysaccharide (LPS)-challenged mice model, and explore the underlying mechanisms. Tail suspension test and forced swimming test were used to evaluate the depressive-like behaviors. SIRT1 mRNA expression was assessed by PCR. Read More

View Article and Full-Text PDF

Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment.

Clin Oral Investig 2021 Jun 11. Epub 2021 Jun 11.

Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School - State University of Campinas, UNICAMP, Av Limeira 901, Piracicaba, São Paulo, Brazil.

Objectives: This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group - GI). It also aimed to compare the findings with those presented by asymptomatic necrotic root canals (asymptomatic group - GII) in the different stages of the endodontic treatment.

Materials And Methods: Microbiological samples were collected from 20 root canals, including purulent collection from acute apical abscesses, before and after chemo-mechanical preparation (CMP) preparation (CMP) with chlorhexidine gel 2% and after 30 days of intracanal medication (ICM) with (Ca[OH]) + chlorhexidine. Read More

View Article and Full-Text PDF

[Effects of resveratrol-mediated inhibition of NOD-like receptor protein 3 inflammasomevia activating silent information regulator 1 on the injury of intestinal mucosal barrier function after sepsis].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):535-540

Department of Critical Care Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China. Corresponding author: Yu Xiangyou, Email:

Objective: To explore whether resveratrol (RSV) could activate silent information regulator 1 (SIRT1) to regulate the activation of NOD-like receptor protein 3 (NLRP3) inflammasome in sepsis induced intestinal injury model, and then reduce intestinal inflammation and cell apoptosis, so as to play a protective role in intestinal barrier function.

Methods: (1) In vitro experiment: human Colorectal adenocarcinoma cells (Caco-2) were cultured, which were divided into normal group (normal culture on complete medium for 48 hours), lipopolysaccharide (LPS) group (normal culture on complete medium for 24 hours, then LPS containing 2 mg/L complete medium intervention for 6 hours), RSV low, medium and high concentration groups and SIRT1 inhibitor (EX-527) group (complete medium normal culture for 24 hours, LPS containing 2 mg/L complete medium intervention for 6 hours, followed by RSV 10, 20, 40 μmol/L or EX-527 10 μmol/L intervention for 6 hours, respectively). The levels of tumor necrosis factor-α (TNF-α) and interleukins (IL-6, IL-18, IL-1β) in the cell supernatant were determined by enzyme linked immunosorbent assay (ELISA). Read More

View Article and Full-Text PDF

[Effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):529-534

Department of Critical Care Medicine, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology (Xiaogan Central Hospital), Xiaogan 432000, Hubei, China. Corresponding author: Yao Peng, Email:

Objective: To evaluate the effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats.

Methods: Forty male Sprague-Dawley (SD) rats were selected and intraperitoneal injection of 10 mg/kg lipopolysaccharide (LPS) was used to establish sepsis model. 0, 30, 45, 60, 75 dB noise stimulation or 0, 50, 100, 200, 400 Lux light stimulation were given to rats (all n = 4). Read More

View Article and Full-Text PDF

The Notch receptor-ligand Delta is involved in the immune response of Penaeus vannamei.

Dev Comp Immunol 2021 Jun 7:104147. Epub 2021 Jun 7.

Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China. Electronic address:

In the Notch signaling pathway in vertebrates and invertebrates, the ligand Delta plays crucial roles in cell proliferation, differentiation, and immunity. Although the Notch signaling pathway has recently been implicated in the immune defense of Penaeus vannamei, the association of Delta with this immune response remains unclear. Here, we cloned and characterized the Delta homolog in P. Read More

View Article and Full-Text PDF

Effect of IL-15-Mediating IFN-γ on HTR-8/SVneo Cells and a Preeclampsia Mouse Model Induced by Lipopolysaccharides.

Xiao-Lu Wang

Gynecol Obstet Invest 2021 Jun 9:1-10. Epub 2021 Jun 9.

Department of Obstetrics, Yantai Yantaishan Hospital, Yantai, China.

Objective: Determine the effect of interleukin (IL)-15 on HTR-8/SVneo cells and a preeclampsia (PE) mouse model induced by LPS.

Methods: Transwell and Annexin-V-FITC/PI assays were performed in HTR-8/SVneo cells transfected with IL-15 activation plasmid/siRNA prior to LPS treatment. Additionally, pregnant mice were injected with LPS and IL-15 siRNA followed by measurement of systolic blood pressure (SBP), urine protein, and serum NO. Read More

View Article and Full-Text PDF

Gastrodin alleviates inflammatory injury of cardiomyocytes in septic shock mice via inhibiting NLRP3 expression.

In Vitro Cell Dev Biol Anim 2021 May 9;57(5):571-581. Epub 2021 Jun 9.

Department of Intensive Care Unit, Taizhou First People's Hospital, No. 218 Hengjie Road, Huangyan District, Taizhou City, 318020, Zhejiang Province, China.

Septic shock leads to myocardial dysfunction and induces inflammation. Nod-like receptor family pyrin domain-containing 3 (NLRP3) inflammasomes are involved in inflammation, and gastrodin can inhibit the activity of inflammasomes. Our study aimed to explore the effect of gastrodin against septic shock-induced injury through inhibiting NLRP3. Read More

View Article and Full-Text PDF

Dasatinib protects against acute respiratory distress syndrome via Nrf2-regulated M2 macrophages polarization.

Drug Dev Res 2021 Jun 9. Epub 2021 Jun 9.

Intensive Care Unit, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Dasatinib, a tyrosine kinase inhibitor, has a protective effect on experimental acute respiratory distress syndrome (ARDS). This study investigated the effect and mechanism of dasatinib in ARDS. C57BL/6 mice were administered with dasatinib (1 and 10 mg/kg) after lipopolysaccharide (LPS) treatment to evaluate the effect of dasatinib on white blood cells (WBC), neutrophils, lymphocytes and macrophages in bronchoalveolar lavage fluid (BALF). Read More

View Article and Full-Text PDF

EZH2 Mediates miR-146a-5p/HIF-1 to Alleviate Inflammation and Glycolysis after Acute Spinal Cord Injury.

Mediators Inflamm 2021 19;2021:5591582. Epub 2021 May 19.

Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.

Acute spinal cord injury (ASCI) is a severe traumatic disease of the central nervous system, the underlying mechanism of which is unclear. This study was intended to study the role of EZH2 and miR-146a-5p/HIF-1 in inflammation and glycolysis after ASCI, providing reference and basis for the clinical treatment and prognosis of ASCI injury. We used lipopolysaccharide (LPS) to induce inflammation of microglia, and we constructed the ASCI animal model. Read More

View Article and Full-Text PDF

Circ_0091702 serves as a sponge of miR-545-3p to attenuate sepsis-related acute kidney injury by upregulating THBS2.

J Mol Histol 2021 Jun 8. Epub 2021 Jun 8.

Department of Emergency Medicine, Taizhou First People's Hospital, No. 218 Hengjie Road, Huangyan District, Taizhou, 318020, Zhejiang, China.

Circular RNA (circRNA) has been shown to play an important function in the progression of human diseases, including sepsis with acute kidney injury (AKI). However, the role and mechanism of circ_0091702 in sepsis-induced AKI have yet to be confirmed. Lipopolysaccharide (LPS) was used to induce HK2 cells to construct AKI cell models in vitro. Read More

View Article and Full-Text PDF

The Role of Type I Interferon Signaling in Regulating Cytokine Production and Cell Survival in Bone Marrow-Derived Macrophages.

Viral Immunol 2021 Jun 7. Epub 2021 Jun 7.

Department of Pathobiology and University of Guelph, Guelph, Canada.

During viral infections, cells produce type I interferons (IFNs), which are detected by the IFN/ receptor (IFNAR). To survive in hosts, viruses have strategies to downregulate IFN-mediated signaling. We hypothesized that macrophages, which are among the first myeloid cells to respond to viral infections, would produce a different cytokine profile if responding to ligation of pattern recognition receptors (PRRs) while IFNAR-mediated signaling was compromised. Read More

View Article and Full-Text PDF

NLRP3 ablation enhances tolerance in heat stroke pathology by inhibiting IL-1β-mediated neuroinflammation.

J Neuroinflammation 2021 Jun 6;18(1):128. Epub 2021 Jun 6.

Department of Lung Disease, Shenzhen Third People's Hospital, The Second Affiliated Hospital, Southern University of Science and Technology, No.29 Bulan Road, Longgang District, Shenzhen, 518055, China.

Background: Patients with prior illness are more vulnerable to heat stroke-induced injury, but the underlying mechanism is unknown. Recent studies suggested that NLRP3 inflammasome played an important role in the pathophysiology of heat stroke.

Methods: In this study, we used a classic animal heat stroke model. Read More

View Article and Full-Text PDF

YTHDF2 mediates LPS-induced osteoclastogenesis and inflammatory response via the NF-κB and MAPK signaling pathways.

Cell Signal 2021 Jun 2;85:110060. Epub 2021 Jun 2.

Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. Electronic address:

Aberrant elevation of osteoclast differentiation and function is responsible for disrupting bone homeostasis in various inflammatory bone diseases. YTH domain family 2 (YTHDF2) is a well-known mA-binding protein that plays an essential role in regulating cell differentiation and inflammatory processes by mediating mRNA degradation. However, the regulatory role of YTHDF2 in inflammatory osteoclast differentiation remains unelucidated. Read More

View Article and Full-Text PDF

Shikonin ameliorates injury and inflammatory response of LPS-stimulated WI-38 cells via modulating the miR-489-3p/MAP2K1 axis.

Environ Toxicol 2021 Jun 5. Epub 2021 Jun 5.

Department of Comprehensive ICU, Luoyang Central Hospital, Luoyang 471009, Henan, China.

Pneumonia is an inflammatory disease induced by infection with different pathogens. Currently, multiple preclinical studies have revealed that shikonin, a natural naphthoquinone, can mitigate lipopolysaccharide (LPS)-induced inflammation, but its underlying mechanism in pneumonia remains unknown. This research was designed to explore the function and regulatory mechanism of shikonin in LPS-induced cell injury and inflammation in WI-38 cells. Read More

View Article and Full-Text PDF

Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.

PLoS One 2021 4;16(6):e0252947. Epub 2021 Jun 4.

Animal Model Research Group, Korea Institute of Toxicology, Jeongeup, Jeollabuk-do, Republic of Korea.

Minipigs have remarkably similar physiology to humans, therefore, they it can be a good animal model for inflammation study. Thus, the conventional (serum chemistry, histopathology) and novel analytic tools [immune cell identification in tissue, cytokine level in peripheral blood mononuclear cells (PBMC) and serum, NF-kB target gene analysis in tissue] were applied to determine inflammation in Chicago Miniature Swine (CMS) minipig. Lipopolysaccharide (LPS)-induced acute systemic inflammation caused liver and kidney damage in serum chemistry and histopathology. Read More

View Article and Full-Text PDF

Ubiquitin-Specific Protease 14 (USP14) Aggravates Inflammatory Response and Apoptosis of Lung Epithelial Cells in Pneumonia by Modulating Poly (ADP-Ribose) Polymerase-1 (PARP-1).

Inflammation 2021 Jun 3. Epub 2021 Jun 3.

Department of Pediatrics, Yijishan Hospital, Wannan Medical College, No. 2, West Road of Zheshan, Jinghu District, Anhui, 241001, Wuhu, China.

Pneumonia is one of the common respiratory diseases in pediatrics. Ubiquitin-specific protease 14 (USP14) contributes the progress of inflammation-associated diseases. Poly (ADP-ribose) polymerase-1 (PARP-1) involves in the signal transduction of inflammatory pulmonary disease. Read More

View Article and Full-Text PDF

Dexmedetomidine Mitigated NLRP3-Mediated Neuroinflammation the Ubiquitin-Autophagy Pathway to Improve Perioperative Neurocognitive Disorder in Mice.

Front Pharmacol 2021 17;12:646265. Epub 2021 May 17.

Department of Anesthesiology, The Second Affiliated Hosptial of Nanchang Univerisity, Nanchang, China.

Surgery and anesthesia-induced perioperative neurocognitive disorder (PND) are closely related to NOD-like receptors (NLR) family, pyrin domain containing 3 (NLRP3) inflammasome microglia inflammatory response. Inhibiting the occurrence of neuroinflammation is an important treatment method to improve postoperative delirium. Fewer NLRP3-targeting molecules are currently available in the clinic to reduce the incidence of postoperative delirium. Read More

View Article and Full-Text PDF

Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.

Int J Mol Sci 2021 May 24;22(11). Epub 2021 May 24.

School of Medicine, Faculty of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.

The conditioned medium of induced pluripotent stem cells (iPSC-CM) can attenuate neutrophil recruitment and endothelial leakage of lipopolysaccharide (LPS)-induced acute lung injury (ALI). Therefore, we investigated the mechanisms by which iPSC-CM regulate the interaction between neutrophils and the endothelium in ALI. Murine iPSCs (miPSCs) were delivered intravenously to male C57BL/6 mice (8-12 weeks old) 4 h after intratracheal LPS injection. Read More

View Article and Full-Text PDF

Resveratrol-Loaded Lipid-Core Nanocapsules Modulate Acute Lung Inflammation and Oxidative Imbalance Induced by LPS in Mice.

Pharmaceutics 2021 May 10;13(5). Epub 2021 May 10.

Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-360, Brazil.

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are inflammatory and oxidative imbalance lung conditions with no successful pharmacological therapy and a high mortality rate. Resveratrol (RSV) is a plant-derived stilbene that presents anti-inflammatory and antioxidant effects. However, its therapeutic application remains limited due to its poor bioavailability, which can be solved by the use of nanocarriers. Read More

View Article and Full-Text PDF

Differential mRNA and miRNA Profiles Reveal the Potential Roles of Genes and miRNAs Involved in LPS Infection in Chicken Macrophages.

Genes (Basel) 2021 May 17;12(5). Epub 2021 May 17.

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Lipopolysaccharide (LPS) is a component of the cell wall of Gram-negative bacteria, and triggers an inflammatory response both in vitro and in vivo. Here, we used LPS from serotype enteritidis to stimulate chicken macrophages (HD11) and conducted the transcriptome analysis using a bioinformatics approach to explore the functions of immune-related genes and miRNAs. In total, 1759 differentially expressed genes (DEGs) and 18 differentially expressed (DE)-miRNAs were detected during LPS infection. Read More

View Article and Full-Text PDF

Antifungal and Anti-Inflammatory Potential of subsp. (Brot.) H.Wolff Essential Oil.

Antibiotics (Basel) 2021 May 17;10(5). Epub 2021 May 17.

Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal.

Fungal infections remain a major health concern with aromatic plants and their metabolites standing out as promising antifungal agents. The present study aims to assess, for the first time, the antifungal and anti-inflammatory potential of subsp. (Brot. Read More

View Article and Full-Text PDF

Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264.7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation.

Molecules 2021 May 7;26(9). Epub 2021 May 7.

Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin 300457, China.

Shanxi-aged vinegar, a traditional Chinese grain-fermented food that is rich in polyphenols, has been shown to have therapeutic effects on a variety of diseases. However, there has been no comprehensive evaluation of the anti-inflammatory activity of polyphenols extracted from Shanxi-aged vinegar (SAVEP) to date. The anti-inflammatory activities of SAVEP, both in RAW 264. Read More

View Article and Full-Text PDF

BAM15, a Mitochondrial Uncoupling Agent, Attenuates Inflammation in the LPS Injection Mouse Model: An Adjunctive Anti-Inflammation on Macrophages and Hepatocytes.

J Innate Immun 2021 Jun 1:1-17. Epub 2021 Jun 1.

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Controlof immune responses through the immunometabolism interference is interesting for sepsis treatment. Then, expression of immunometabolism-associated genes and BAM15, a mitochondrial uncoupling agent, was explored in a proinflammatory model using lipopolysaccharide (LPS) injection. Accordingly, the decreased expression of mitochondrial uncoupling proteins was demonstrated by transcriptomic analysis on metabolism-associated genes in macrophages (RAW246. Read More

View Article and Full-Text PDF

A20 functions as a negative regulator in macrophage for DSS-induced colitis.

Int Immunopharmacol 2021 May 29;97:107804. Epub 2021 May 29.

Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou 450052, Henan, China. Electronic address:

The function of A20 as a deubiquitinating enzyme in inflammatory diseases and autoimmune diseases has been reported, we therefore aimed to investigate the potential effects of A20 in macrophages and dextran sodium sulfate (DSS)-induced colitis mouse model. Colitis mouse model was induced by DSS treatment. Tnfaip3 mice were crossed with Lyz2-Cre mice to generate A20 myeloid cell-conditional knockout mice. Read More

View Article and Full-Text PDF

Large extracellular vesicles in the left atrial appendage in patients with atrial fibrillation-the missing link?

Clin Res Cardiol 2021 Jun 1. Epub 2021 Jun 1.

Medical Department II, University Hospital Bonn, Venusberg-Campus 1, 53127, Bonn, Germany.

Atrial fibrillation (AF) is the most frequent arrhythmic disease in humans, which leads to thrombus formation in the left atrial appendage and stroke through peripheral embolization. Depending on their origin, large extracellular vesicles (lEVs) can exert pro-coagulant functions. In the present study, we investigated how different types of AF influence the levels of large EV subtypes in three distinct atrial localizations. Read More

View Article and Full-Text PDF

[Establishment and evaluation of hepatocyte injury model induced by LPS/D-galactosamine in vitro].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Jun;37(6):495-500

Institute of Military Cognition and Brain Science, Academy of Military Medical Sciences, Beijing 100850, China. *Corresponding author, E-mail:

Objective To establish a novel hepatocyte injury model induced by lipopolysaccharide/D-galactosamine (LPS/D-GalN) in vitro. Methods Freshly isolated mouse primary hepatocytes were cultured in vitro and treated with different doses of tumor necrosis factor-α (TNF-α) and 5 mg/mL of D-GalN. The supernatants from hepatocyte culture were detected for alanine aminotransferase (ALT) activity by chemiluminescence assay. Read More

View Article and Full-Text PDF

[Mannose-capped lipoarabinomannan (ManLAM) binding TLR2 activates mast cells to release exosomes and induces M2 polarization of macrophages].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Jun;37(6):481-486

Department of Immunology and Microbiology, Wannan Medical College, Wuhu 241000, China. *Corresponding author, E-mail:

Objective To explore the mechanism of mannose-capped lipoarabinomannan (ManLAM) lipopolysaccharide of mycobacterium tuberculosis (MTB) inducing exosomes of mast cells to recruit macrophages and influence macrophage polarization for immune evasion. Methods H37Rv MTB was cultured and ManLAM extracted and identified. The extracted ManLAM was used to treat mast cells, and the exosomes secreted by mast cells were collected. Read More

View Article and Full-Text PDF