623 results match your criteria detected immunochromatographic

Rapid Detection of PBP2a in Staphylococci from Shortly Incubated Subcultures of Positive Blood Cultures by an Immunochromatographic Assay.

Microbiol Spectr 2021 Jul 28:e0046221. Epub 2021 Jul 28.

University of Zurichgrid.7400.3, Institute of Medical Microbiology, Zurich, Switzerland.

Staphylococcus aureus, as well as coagulase-negative staphylococci (CoNS), can cause a wide range of human infections both in nosocomial and community settings. Βeta-lactams are the antibiotics of choice for the treatment of bloodstream infections (BSI) caused by these microorganisms. Resistance to virtually all β-lactams (also referred to as methicillin resistance) primarily results from the production of an alternative penicillin-binding protein (PBP2a) encoded by the gene. Read More

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The evaluation of a novel digital immunochromatographic assay with silver amplification to detect SARS-CoV-2.

J Infect Chemother 2021 Jul 13. Epub 2021 Jul 13.

Department of Infectious Diseases, University of Tsukuba Hospital, 2-1-1 Amakubo, Tsukuba, Ibaraki, 305-8576, Japan; Department of Infectious Diseases, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.

Introduction: Rapid antigen tests are convenient for diagnosing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); however, they have lower sensitivities than nucleic acid amplification tests. In this study, we evaluated the diagnostic performance of Quick Chaser Auto SARS-CoV-2, a novel digital immunochromatographic assay that is expected to have higher sensitivity than conventional antigen tests.

Methods: A prospective observational study was conducted between February 8 and March 24, 2021. Read More

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Emergent canine visceral leishmaniasis in Argentina: Comparative diagnostics and relevance to proliferation of human disease.

PLoS Negl Trop Dis 2021 Jul 19;15(7):e0009552. Epub 2021 Jul 19.

Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.

Background: Visceral leishmaniasis (VL) is a zoonotic protozoal vector-borne disease that is a major public health challenge. In Argentina, canine (CVL) and human visceral leishmaniasis (HVL) have recently emerged. There is a lack of standardised diagnostic tests for CVL, which hinders control of CVL and HVL. Read More

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Carbapenemase detection by NG-Test CARBA 5-a rapid immunochromatographic assay in carbapenem-resistant diagnosis.

Ann Transl Med 2021 May;9(9):769

Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The global spread of carbapenem-resistant (CRE) represents a serious public health concern as these organisms are associated with limited treatment options, high mortality rate and rapid transmissibility. The identification of carbapenemase remains a challenge in microbiological laboratories as no single method is perfect when considering cost, carbapenemase coverage, accuracy, handling complexity and TATs together.

Methods: NG-Test CARBA 5 assay and modified carbapenem inactivation method in conjunction with EDTA carbapenem inactivation method (mCIM/eCIM) were challenged with a collection of 299 molecularly characterized CRE isolates in China in order to evaluate the performance in detecting five major carbapenemases ( , , , , and ) among Enterobacterales. Read More

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Evaluation of Rapid Antibody Test and Chest Computed Tomography Results of COVID-19 Patients: A Retrospective Study.

J Med Virol 2021 Jul 14. Epub 2021 Jul 14.

Department of Infectious Diseases and Clinical Microbiology, Nigde Omer Halisdemir University Training and Research Hospital, Nigde, Turkey.

Objective: The purpose of this study was to evaluate the SARS-CoV-2 IgM/IgG rapid antibody test results in symptomatic patients with COVID-19 and their chest computed tomography (CT) data.

Methods: A total of 320 patients admitted to our hospital for different durations due to COVID-19 were included in the study. Serum samples were obtained within 0 to 7 days from COVID-19 patients confirmed by RT-PCR and chest CT scan. Read More

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Well-orientation strategy for direct binding of antibodies: Development of the immunochromatographic test using the antigen modified FeO nanoprobes for sensitive detection of aflatoxin B.

Food Chem 2021 Mar 21;364:129583. Epub 2021 Mar 21.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:

One of the major concerns in the application of nanocarriers in biosensing is the impair of the recognition molecules bioactivity loaded on their surfaces due to harsh and laborious cross-linking and random orientation, resulting in unsatisfactory sensitivity. Herein, we proposed a novel immunochromatographic test strip (FNS-ag-DICTS) by taking advantage of the antigen (ag) modified FeO nanostructures (FNSs) as new signal tags and goat anti-mouse IgG labeling on the detection line instead of ag, which was used for sensitive detection of aflatoxin B (AFB). The fabricated FNS-ag can orientate the Fab region of monoclonal antibodies (mAbs), waiving the intrinsic limitations of traditional nanomaterials labeled mAbs. Read More

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Seroprevalence of Brucella canis in canines from a dog shelter in Bogotá, Colombia

Biomedica 2021 06 29;41(2):260-270. Epub 2021 Jun 29.

Facultad de Medicina Veterinaria, Fundación Universitaria Agraria de Colombia, Bogotá, D.C., Colombia.

Introduction: The risk of Brucella canis infection in humans and dogs has increased due to the permanent exposure to asymptomatic carrier dogs. In Colombia, there is evidence of B. canis infection in humans living with dogs. Read More

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Immunological Cross-Reactivity of Proteins Extracted from the Oomycete and the Fungus Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis.

J Fungi (Basel) 2021 Jun 11;7(6). Epub 2021 Jun 11.

Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.

Pythiosis, a life-threatening disease caused by , has been increasingly diagnosed worldwide. A recently developed immunochromatographic test (ICT) enables the rapid diagnosis of pythiosis. During the 3-year clinical implementation of ICT in Thailand, we collected the laboratory reports of 38 animals with suspected pythiosis and detected ICT false-positive results in three horses and a dog with basidiobolomycosis. Read More

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Diagnostic Performance of a Rapid Immunochromatographic Test Kit for Detecting Canine Parvovirus Infection.

Top Companion Anim Med 2021 Jun 11;45:100551. Epub 2021 Jun 11.

Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.

In dogs, canine parvovirus (CPV) enteritis is associated with high morbidity and fatality rates requiring early diagnosis to facilitate treatment and reduce its spread. In recent times, various commercial immunochromatographic (IC) test kits are available for its rapid diagnosis, which require an assessment of their accuracy. Therefore, precision of a point-of-care IC combination test kit for canine coronavirus (CCoV)/CPV faecal antigen detection was evaluated in this study. Read More

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SARS-CoV-2 in asymptomatic pregnant women in South Brazil: RT-PCR and serological detection.

J Perinat Med 2021 Jul 10;49(6):717-722. Epub 2021 Jun 10.

Department of Obstetrics and Gynecology, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, Brazil.

Objectives: This study aims to detect the SARS-CoV-2 infection prevalence in asymptomatic pregnant women.

Methods: A group of 195 asymptomatic pregnant women who attended the prenatal care outclinic and to the obstetric emergency department was tested concomitantly for SARS-CoV-2 by RT-PCR and serological tests.

Results: The virus was detected by RT-PCR in two (1. Read More

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Immunochromatographic Assay Based on Polydopamine-Decorated Iridium Oxide Nanoparticles for the Rapid Detection of Salbutamol in Food Samples.

ACS Appl Mater Interfaces 2021 Jun 9;13(24):28899-28907. Epub 2021 Jun 9.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, P. R. China.

Salbutamol (SAL), a β-2 adrenoreceptor agonist, is an unpopular addition to livestock and poultry, causing several side effects to human health. Thus, it is very important to develop a simple and rapid analytical method to screen SAL in the field of food safety. Here, we present an immunochromatographic assay (ICA) method for sensitively detecting SAL with polydopamine-decorated iridium oxide nanoparticles ([email protected] NPs) as a signal tag. Read More

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Colloidal gold immunochromatographic assay (GICA) is an effective screening method for identifying detectable anti-SARS-CoV-2 neutralizing antibodies.

Int J Infect Dis 2021 Jul 6;108:483-486. Epub 2021 Jun 6.

Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University, Beijing 100191, China. Electronic address:

Introduction: A large number of COVID-19 patients are in recovery, and millions of people are vaccinated for COVID-19 globally. This calls for a rapid screening strategy of SARS-CoV-2 protective antibodies, generated in rehabilitated and vaccinated populations.

Methods: Serum samples collected over a follow-up period of six months from 306 COVID-19 cases discharged from Wuhan Tongji Hospital were analyzed. Read More

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Development of a Prototype Lateral Flow Immunoassay of Cortisol in Saliva for Daily Monitoring of Stress.

Biosensors (Basel) 2021 May 7;11(5). Epub 2021 May 7.

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049 Saratov, Russia.

Emotional stress negatively affects the quality of a person's daily life. From a physiological point of view, stress is expressed in the excitation of the hypothalamic-pituitary-adrenal cortex axis, which leads to the release of the hormone cortisol into the blood. We developed a lateral flow immunoassay to detect cortisol in human salivary fluid and tested it on 10 healthy volunteers daily for about one month ( = 293 saliva samples). Read More

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Serological Evidence of Human Orthohantavirus Infections in Barbados, 2008 to 2016.

Pathogens 2021 May 8;10(5). Epub 2021 May 8.

Best-dos Santos Public Health Laboratory, Enmore #6, Lower Collymore Rock, St. Michael BB11155, Barbados.

Background: Hantavirus pulmonary syndrome (HPS) is well-known in South and North America; however, not enough data exist for the Caribbean. The first report of clinical orthohantavirus infection was obtained in Barbados, but no other evidence of clinical orthohantavirus infections among adults in the Caribbean has been documented.

Methods: Using enzyme linked immunosorbent assay (ELISA) tests followed by confirmatory testing with immunofluorescent assays (IFA), immunochromatographic (ICG) tests, and pseudotype focus reduction neutralization tests (pFRNT), we retrospectively and prospectively detected orthohantavirus-specific antibodies among patients with febrile illness in Barbados. Read More

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Identification and in Sewage Water-A Pilot Study.

Viruses 2021 05 6;13(5). Epub 2021 May 6.

Departament of Viral Infections, "Cantacuzino" Medico Military National Institute of Research and Development, 050096 Bucharest, Romania.

Due to the outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), combined with the risk of polio importation from Ukraine, we evaluated the presence of SARS-CoV-2 and enteroviruses in 25 sewage water samples from Romania, concentrated using the WHO method between January 2020 and January 2021. Surveillance for enteroviruses and SARS-CoV-2 are relevant in the calculation of prevalence estimates as well as early detection of the introduction or disappearance of these viruses. For SARS-CoV-2 detection, we used two immunochromatographic nucleocapsid antigenic tests as well as real-time PCR assays, produced for respiratory samples. Read More

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Role of rotavirus vaccination on G9P[8] rotavirus strain during a seasonal outbreak in Japan.

Hum Vaccin Immunother 2021 May 25:1-6. Epub 2021 May 25.

Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.

Although two live oral rotavirus (RV) vaccines, Rotarix and RotaTeq, play a critical role toward reducing disease severity, hospitalization, and death rate in RV infections, regular monitoring of vaccine effectiveness (VE) is yet necessary because the segmented genome structure and reassortment capability of RVs pose considerable threats toward waning VE. In this study, we examined the VE by a test-negative study design against G9P[8]I2 strain during a seasonal outbreak in February-May, 2018, in an outpatient clinic in Kyoto Prefecture, Japan. It remains important because G9P[8]I2 strain remains partially heterotypic to these vaccines and predominating in post-vaccination era. Read More

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Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2.

J Virol Methods 2021 08 11;294:114183. Epub 2021 May 11.

Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan. Electronic address:

Background: The novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the worldwide coronavirus disease-19 (COVID-19) pandemic, starting in late 2019. The standard diagnostic methods to detect SARS-CoV-2 are PCR-based genetic assays. Antigen-antibody-based immunochromatographic assays are alternative methods of detecting this virus. Read More

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Production of polyclonal antibody against the recombinant PirB protein of Vibrio parahaemolyticus.

J Genet Eng Biotechnol 2021 May 11;19(1):70. Epub 2021 May 11.

University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.

Background: Acute hepatopancreatic necrosis disease (AHPND) is caused by toxin-producing strains of Vibrio parahaemolyticus which contain deadly binary toxins PirA and PirB encoded in pVA1 plasmid. The polyclonal antibodies against PirB protein could be used to develop immunochromatographic test strip for in-field diagnosis of AHPND.

Results: In this study, PirB gene was amplified, cloned, and expressed in E. Read More

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A Rapid Intraoperative Parathyroid Hormone Assay Based on the Immune Colloidal Gold Technique for Parathyroid Identification in Thyroid Surgery.

Front Endocrinol (Lausanne) 2020 22;11:594745. Epub 2021 Apr 22.

Department of Thyroid and Breast Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: A novel immunochromatographic test strip method was developed to detect tissue parathyroid hormone (PTH) using the immune colloidal gold technique (ICGT). The accuracy and application value of this method for intraoperative parathyroid identification were evaluated.

Methods: Serum samples were collected to measure PTH by both ICGT and electrochemiluminescence immunoassay (ECLIA). Read More

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Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay.

Microorganisms 2021 Apr 27;9(5). Epub 2021 Apr 27.

Clinical Microbiology Laboratory, Medical School, "Attikon" University General Hospital, National and Kapodistrian University of Athens, 1 Rimini Street, 12462 Athens, Greece.

We report a preliminary evaluation of the NG-Test CARBA 5 immunochromatographic assay for detecting carbapenemases directly from rectal swabs on the same day of sampling. Thirty fecal swabs were examined for carbapenemase-producing organisms (CPOs) by conventional culture, PCR, and NG-Test CARBA 5. Each sample was tested by the immunochromatographic assay five times, including direct testing and incubation in trypticase soy broth for 1, 2, 3, and 4 h. Read More

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Comparison of four PCR and two point of care assays used in the laboratory detection of SARS-CoV-2.

J Virol Methods 2021 07 16;293:114165. Epub 2021 Apr 16.

Military Medical Centre of the Hungarian Defence Forces, H-1134, Budapest, Róbert Károly krt. 44, Hungary; Semmelweis University - Faculty of Medicine, H-1085, Budapest, Üllői út 26., Hungary. Electronic address:

Seeing the global emergence and the lack of a definitive cure for COVID-19, it is essential to find the most sensitive and specific detection method to identify infected patients in a timely manner. Our paper aims to compare the clinical sensitivity of different commercial RT-qPCR (Genesig, 1copy, DNA-Techonolgy and Charité primer-probe sets), isothermal PCR (Ustar Isothermal Amplification-Real Time Fluorescent Assay) and immunochromatographic antigen detection (BIOCREDIT COVID-19 Ag) assays developed to use in laboratory diagnosis of COVID-19. A total of 119 nasopharyngeal swab specimens were collected from symptomatic patients. Read More

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Occurrence, distribution and genomic characteristics of plum pox virus isolates from common apricot (Prunus armeniaca) and Japanese apricot (P. mume) in China.

Plant Dis 2021 Apr 15. Epub 2021 Apr 15.

Chinese Academy of Agricultural Sciences Institute of Plant Protection, 243827, Beijing, China;

Plum pox or Sharka disease, caused by infection with plum pox virus (PPV), results in enormous economic losses to the stone fruit industry. However, the frequency and distribution of PPV remains unclear in China, the world's largest stone fruit producer. Systemic visual surveys were performed on stone fruit trees in China from 2008 to 2018, and the results suggest that plum pox disease is widely distributed on common apricots (Prunus armeniaca) and Japanese apricots (P. Read More

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Development of an immunochromatographic strip for rapid detection of H7 subtype avian influenza viruses.

Virol J 2021 Apr 7;18(1):68. Epub 2021 Apr 7.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.

Background: H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. H7N9 AIV has a high mortality rate after infection in humans, and most human cases have close contacted with poultry in the live poultry market. Therefore, it is necessary to develop a rapid point-of-care testing (POCT) technique for H7N9 AIV detection. Read More

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Seroprevalence of anti-SARS-CoV-2 antibodies in Japanese COVID-19 patients.

PLoS One 2021 6;16(4):e0249449. Epub 2021 Apr 6.

Department of General Medicine, Juntendo University Faculty of Medicine, Tokyo, Japan.

Objectives: To determine the seroprevalence of anti-SARS-CoV-2 IgG and IgM antibodies in symptomatic Japanese COVID-19 patients.

Methods: Serum samples (n = 114) from 34 COVID-19 patients with mild to critical clinical manifestations were examined. The presence and titers of IgG antibody for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were determined by a chemiluminescent microparticle immunoassay (CMIA) using Alinity i SARS-CoV-2 IgG and by an immunochromatographic (IC) IgM/IgG antibody assay using the Anti-SARS-CoV-2 Rapid Test. Read More

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Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies.

Pathogens 2021 Mar 18;10(3). Epub 2021 Mar 18.

Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.

Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. Read More

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Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection.

Nanomaterials (Basel) 2021 Mar 18;11(3). Epub 2021 Mar 18.

Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea.

Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. Read More

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Four-Hour Immunochromatographic Detection of Intestinal Carriage of Carbapenemase-Producing : a Validation Study.

J Clin Microbiol 2021 05 19;59(6). Epub 2021 May 19.

Département d'Anesthésie-Réanimation et Médecine Périopératoire, Sorbonne Université APHP.6, GRC 29, DMU DREAM, Hôpital Tenon, Paris, France

The increasing incidence of carbapenemase-producing Gram-negative bacilli (C-PGNB) represents a major public health challenge. Rapid detection of digestive colonization with C-PGNB is fundamental to control their spread. We performed the validation of a rapid protocol for C-PGNB detection directly on rectal swabs. Read More

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Comparison of Anti-Dengue and Anti-Zika IgG on a Plasmonic Gold Platform with Neutralization Testing.

Am J Trop Med Hyg 2021 Mar 29. Epub 2021 Mar 29.

2Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia.

Antibody cross-reactivity confounds testing for dengue virus (DENV) and Zika virus (ZIKV). We evaluated anti-DENV and anti-ZIKV IgG detection using a multiplex serological platform (the pGOLD assay) in patients from the Asunción metropolitan area in Paraguay, which experiences annual DENV outbreaks but has reported few autochthonous ZIKV infections. Acute-phase sera were tested from 77 patients who presented with a suspected arboviral illness from January to May 2018. Read More

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Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein.

Front Immunol 2021;12:635677. Epub 2021 Mar 11.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.

The outbreak and worldwide pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have a significant impact on global economy and human health. In order to reduce the disease spread, 16 monoclonal antibodies (McAbs) again SARS-CoV-2 were generated by immunized mice with the spike protein receptor binding domain (RBD), which was expressed in Chinese hamster ovary cell (CHO). A colloidal gold-based immunochromatographic strip was developed with two McAbs to detect SARS-CoV-2 spike protein, which can play a potential role in monitoring vaccine quality. Read More

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Rapid Quantification of Chlorpromazine Residues in Pork Using Nanosphere-Based Time-Resolved Fluorescence Immunoassay Analyzer.

Int J Anal Chem 2021 9;2021:6633016. Epub 2021 Mar 9.

Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MARA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu 210095, China.

Immunochromatographic assays are good analytical tools for the detection of drug residues. We report a nanosphere-based time-resolved fluorescence immunoassay (nano-TRFIA) based on a monoclonal antibody and a portable TRFIA analyzer for the rapid quantification of chlorpromazine (CPZ) residues in pork. Under optimal conditions, the nano-TRFIA detected CPZ residues within 6 min of sample pretreatment. Read More

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