129,671 results match your criteria detect degradation


Proteomic signatures of radioresistance: Alteration of inflammation, angiogenesis and metabolism-related factors in radioresistant oesophageal adenocarcinoma.

Cancer Treat Res Commun 2021 Apr 18;27:100376. Epub 2021 Apr 18.

Department of Surgery, Trinity Translational Medicine Institute, Trinity St. James's Cancer Institute, St. James's Hospital, Trinity College Dublin, Dublin, Ireland.

The clinical management of locally advanced oesophageal adenocarcinoma (OAC) involves neoadjuvant chemoradiotherapy (CRT), but as radioresistance remains a major clinical challenge, complete pathological response to CRT only occurs in 20-30% of patients. In this study we used an established isogenic cell line model of radioresistant OAC to detect proteomic signatures of radioresistance to identify novel molecular and cellular targets of radioresistance in OAC. A total of 5785 proteins were identified of which 251 were significantly modulated in OE33R cells, when compared to OE33P. Read More

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miR-720 Regulates Insulin Secretion by Targeting Rab35.

Biomed Res Int 2021 5;2021:6662612. Epub 2021 Apr 5.

Medical Center of Stomatology, The First Affiliated Hospital, Jinan University, Guangzhou 510630, China.

miRNAs pose a good prospect in the diagnosis and treatment of type 2 diabetes (T2D). This study is aimed at investigating whether miR-720 targets Rab35 to regulate insulin secretion in MIN6 cells and its molecular mechanism and the clinical value of miR-720 as a specific biomarker of T2D. Fifty-five samples of new diagnosis T2D patients and normal control were collected. Read More

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High Methionine Diet-Induced Alzheimer's Disease like Symptoms Are Accompanied by 5-Methylcytosine Elevated Levels in the Brain.

Behav Neurol 2021 7;2021:6683318. Epub 2021 Apr 7.

Key Laboratory of Basic Pharmacology of Ministry of Education, Zunyi Medical University, Guizhou Province, China.

Background: Excessive or insufficient intake of methionine (Met) causes neuronal dysfunction, neurodegeneration, cerebrovascular dysfunction, vascular leakage, and short-term memory loss, which result in the occurrence of Alzheimer's disease- (AD-) like symptoms.

Objective: To determine the relationship between high methionine diets (HMD) induced AD-like symptoms and 5-methylcytosine (5-mC) level.

Methods: C57BL/6J mice were randomly divided into two groups: the control group (Maintain diets) and the model group (2% HMD). Read More

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Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals.

Commun Biol 2021 Apr 20;4(1):486. Epub 2021 Apr 20.

Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA), Bizkaia, Spain.

There is an ongoing need of developing sensitive and specific methods for the determination of SARS-CoV-2 seroconversion. For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows the identification of IgG and IgM antibodies against three immunogenic proteins simultaneously: the spike receptor-binding domain (RBD), the spike protein subunit 1 (S1) and the nucleoprotein (N). Using different cohorts of samples collected before and after the pandemic, we show that this assay is more sensitive than ELISAs performed in our laboratory. Read More

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Urinary microRNA biomarkers for detecting the presence of esophageal cancer.

Sci Rep 2021 Apr 20;11(1):8508. Epub 2021 Apr 20.

Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan.

Esophageal cancer (EC) including esophageal squamous cell carcinoma (ESCC) and adenocarcinoma (EAC) generally exhibits poor prognosis; hence, a noninvasive biomarker enabling early detection is necessary. Age- and sex-matched 150 healthy controls (HCs) and 43 patients with ESCC were randomly divided into two groups: 9 individuals in the discovery cohort for microarray analysis and 184 individuals in the training/test cohort with cross-validation for qRT-PCR analysis. Using 152 urine samples (144 HCs and 8 EACs), we validated the urinary miRNA biomarkers for EAC diagnosis. Read More

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Incidence of cervical intraepithelial lesions and human papilloma virus infection in female renal transplant recipients.

Transpl Infect Dis 2021 Apr 20:e13622. Epub 2021 Apr 20.

Departments of Reproductive Biology, Mexico.

Background: Female renal transplant recipients (RTR) are at high risk of human papillomavirus (HPV)-related anogenital premalignancies and cancer. The aim of this study was to estimate the incidence of cervical intraepithelial lesions (IL) and HPV infection, and their associated factors, in Mexican RTR.

Methods: This is a prospective cohort study conducted between January 2011 and December 2017. Read More

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Electrochemical measurements reveal reactive oxygen species in stress granules.

Angew Chem Int Ed Engl 2021 Apr 19. Epub 2021 Apr 19.

Chalmers Institute of Technology, Chemical and Biological Engineering, Kemivägen 10, 41296, Göteborg, SWEDEN.

Stress granules (SGs) are membrane-less organelles that assemble in the cytoplasm to organize cellular contents and promote rapid adaptation during stress. To understand how SGs contribute to physiological functions, we used electrochemical measurements to detect electroactive species in SGs. With amperometry, we discovered that reactive oxygen species (ROS) are encapsulated inside arsenite-induced SGs, and H 2 O 2 is the main species. Read More

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Effects of O on alder photosynthesis and symbiosis with Frankia.

New Phytol 1989 Apr;111(4):647-656

Department of General Science, Oregon State University, Corvallis, OR, 97331-6505, USA.

Alnus serrulata (Aiton) Willdenow seedlings with and without root nodules formed by the nitrogen-fixing actinomycete Frankia were exposed to clean filtered air or ozone (O ) at 0.12 μl l for 27 d (approximately 164 h total exposure). Gas exchange measurements on leaves and transmission electron micrographs of root nodule cells were made to detect any O effects on the functioning of leaves and the root symbiont. Read More

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High-Density Lipoprotein Cholesterol and Apolipoprotein A1 in Synovial Fluid: Potential Predictors of Disease Severity of Primary Knee Osteoarthritis.

Cartilage 2021 Apr 17:19476035211007919. Epub 2021 Apr 17.

Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, People's Republic of China.

Objectives: The aim of this study was to detect levels of common lipid species in serum and synovial fluid (SF) of primary knee osteoarthritis (OA) patients and investigate their correlations with disease severity.

Materials And Methods: The study enrolled 184 OA patients receiving arthroscopic debridement or total knee arthroplasty and 180 healthy controls between April 2012 and March 2018. Total triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (ApoA1), and apolipoprotein B (ApoB) levels were analyzed in serum and SF of OA patients, and in serum of healthy individuals. Read More

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Single Molecule Assay for Ultrasensitive Detection of Cathepsin B in Human Blood.

ACS Omega 2021 Apr 31;6(14):9609-9616. Epub 2021 Mar 31.

Brain Trauma Neuroprotection Branch, Center for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, Maryland 20910, United States.

Cathepsin B (catB) is a lysosomal cysteine protease expressed in several cells and organs, where it plays a role in protein degradation and turnover. Extracellular, secreted catB has utility as a biomarker for a host of pathological or physiological states, including a myriad of cancers or neurological diseases and injuries. Analytical or diagnostic assessment may be limited by biological sample volume availability. Read More

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The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats.

Front Endocrinol (Lausanne) 2021 2;12:652807. Epub 2021 Apr 2.

Department of Gynaecology, Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University, Beijing, China.

Recurrent spontaneous abortion (RSA) remains a critical and challenging problem in reproduction. To discover novel biomarkers for RSA, ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) metabolomics approach was applied to detect RSA serum metabolic profiles and explore its possible pathogenesis and mechanism. The abortion rat model was established, and a metabolomics analysis was performed to evaluate the differentially expressed metabolites between the control and model groups. Read More

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Effects of the circadian rhythm on milk composition in dairy cows: Does day-milk differ from night-milk?

J Dairy Sci 2021 Apr 14. Epub 2021 Apr 14.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan 450046, People's Republic of China. Electronic address:

Metabolism in most organisms can show variations between the day and night. These variations may also affect the composition of products derived from livestock. The aim of the present study was to investigate the difference in composition between the day-milk and night-milk of dairy cows. Read More

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Identification of metabolism genes related to hepatocarcinogenesis and progression in type 2 diabetes mellitus via co-expression networks analysis.

Hereditas 2021 Apr 17;158(1):14. Epub 2021 Apr 17.

Department of Endocrinology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Background: Type 2 Diabetes Mellitus (T2DM) is an independent risk factor of hepatocellular carcinoma (HCC). However, the related genes and modules to hepatocarcinogenesis and progression in T2DM remain unclear.

Methods: The microarray data from Gene Expression Omnibus (GEO) were analyzed to screen differentially expressed genes (DEGs) of T2DM and HCC dataset. Read More

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Visible-light induced activation of persulfate by self-assembled EHPDI/TiO photocatalyst toward efficient degradation of carbamazepine.

Sci Total Environ 2021 Apr 9;783:146996. Epub 2021 Apr 9.

State Key Laboratory of Hydraulics and Mountain River Engineering, College of Architecture and Environment, Sichuan University, Chengdu 610065, China; Sino-German Centre for Water and Health Research, Sichuan University, Chengdu 610065, China. Electronic address:

Removal of pharmaceutical and personal care products from wastewater is very important in water treatment process. Combining photocatalysis with persulfate (PS) could be a good solvent for this problem. Novel perylene diimide derivative (EHPDI) was designed and synthesized. Read More

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IκBα targeting promotes oxidative stress-dependent cell death.

J Exp Clin Cancer Res 2021 Apr 16;40(1):136. Epub 2021 Apr 16.

Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043, Orbassano, Italy.

Background: Oxidative stress is a hallmark of many cancers. The increment in reactive oxygen species (ROS), resulting from an increased mitochondrial respiration, is the major cause of oxidative stress. Cell fate is known to be intricately linked to the amount of ROS produced. Read More

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Silencing SHMT2 inhibits the progression of tongue squamous cell carcinoma through cell cycle regulation.

Cancer Cell Int 2021 Apr 16;21(1):220. Epub 2021 Apr 16.

Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, 510055, Guangdong, China.

Background: Serine hydroxymethyltransferase 2 (SHMT2) is a vital metabolic enzyme in one carbon metabolism catalyzing the conversion of serine to glycine, which has been reported to play a crucial role in the progression of tumors. However, its function in tongue squamous cell carcinoma (TSCC) remains unclear.

Methods: SHMT2 expression was analyzed using samples in online databases, and was assessed through immunohistochemistry staining of collected clinical specimens. Read More

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Metabolic Profiles of 5F-MDMB-PICA in Human Urine, Serum, and Hair Samples Using LC-Q Exactive HF MS.

J Anal Toxicol 2021 Apr 16. Epub 2021 Apr 16.

Drug and Food Anti-doping Laboratory, China Anti-Doping Agency, 1st Anding Road, Chaoyang District, Beijing 100029, China.

In 2020, 5F-MDMB-PICA (5F-MDMB-2201) was one of the most common synthetic cannabinoids (SCs) identified in drugs seized by the Beijing Drug Control Agency and it was categorized as Schedule II by the United Nations Office on Drugs and Crime (UNODC) in March 2020. It is difficult to detect 5F-MDMB-PICA in biological matrices due to its fast metabolic rate in vivo. In this work, 5F-MDMB-PICA metabolic profiles were investigated by liquid chromatography-Q Exactive HF Hybrid Quadrupole-Orbitrap mass spectrometry (LC-QE-HF-MS), with accurate mass measurements in human urine, serum, and pubic hair. Read More

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Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis.

Cancer Cell Int 2021 Apr 15;21(1):217. Epub 2021 Apr 15.

Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, China.

Background: Lung adenocarcinoma (LUAD) metastasis significantly reduces patient survival; hence inhibiting the metastatic ability of lung cancer cells will greatly prolong patient survival. Alkaline phosphatase (ALPL), a homodimeric cell surface phosphohydrolase, is reported to play a controversial role in prostate cancer and ovarian cancer cell migration; however, the function of ALPL in LUAD and the related mechanisms remain unclear.

Methods: TCGA database was used to analysis the expression of ALPL, and further verification was performed in a cohort of 36 LUAD samples by qPCR and western blot. Read More

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LncRNA-Fendrr protects against the ubiquitination and degradation of NLRC4 protein through HERC2 to regulate the pyroptosis of microglia.

Mol Med 2021 Apr 15;27(1):39. Epub 2021 Apr 15.

Department of Anesthesiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, People's Republic of China.

Objectives: Targeted inhibition of inflammatory response can reduce diabetic cerebral ischemia-reperfusion (I/R) injure. Pyroptosis is characterized by caspase-1 dependence and the release of a large number of pro-inflammatory factors. LncRNA-Fendrr is associated with a variety of diseases, but Fendrr has not been studied in diabetic cerebral I/R. Read More

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Non-invasive assessment of heterogeneity of gliomas using diffusion and perfusion MRI: correlation with spatially co-registered PET.

Acta Radiol 2021 Apr 15:2841851211006913. Epub 2021 Apr 15.

Department of Nuclear Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, PR China.

Background: Heterogeneity of gliomas challenges the neuronavigated biopsy and oncological therapy. Diffusion and perfusion magnetic resonance imaging (MRI) can reveal the cellular and hemodynamic heterogeneity of tumors. Integrated positron emission tomography (PET)/MRI is expected to be a non-invasive imaging approach to characterizing glioma. Read More

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Early detection of osteoarthritis in the rat with an antibody specific to type II collagen modified by reactive oxygen species.

Arthritis Res Ther 2021 Apr 14;23(1):113. Epub 2021 Apr 14.

Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Chaterhouse Square, London, EC1M 6BQ, UK.

Background: Osteoarthritis (OA) is a disease of the whole joint, with articular cartilage breakdown as a major characteristic. Inflammatory mediators, proteases, and oxidants produced by chondrocytes are known to be responsible for driving cartilage degradation. Nevertheless, the early pathogenic events are still unclear. Read More

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Changes of type II collagenase biomarkers on IL-1β-induced rat articular chondrocytes.

Exp Ther Med 2021 Jun 2;21(6):582. Epub 2021 Apr 2.

College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P.R. China.

Osteoarthritis (OA) is characterized by progressive degeneration of cartilage, formation of cartilage at the cartilage edge, and remodeling of the subchondral bone. Pro-inflammatory cytokines [e.g. Read More

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Free-Living, Psychrotrophic Bacteria of the Genus Are Descendants of Pathobionts.

mSystems 2021 Apr 13;6(2). Epub 2021 Apr 13.

Department of Microbiome Science, Max Planck Institute for Developmental Biology, Tübingen, Germany

Host-adapted microorganisms are generally assumed to have evolved from free-living, environmental microorganisms, as examples of the reverse process are rare. In the phylum , family , the genus includes strains from a broad ecological distribution including animal bodies as well as sea ice and other nonhost environments. To elucidate the relationship between these ecological niches and 's evolutionary history, we performed tandem genomic analyses with phenotyping of 85 accessions. Read More

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[Estradiol activates ERK phosphorylation by binding to ERβ to inhibit proliferation and promote apoptosis of human chondrocytes].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Mar;41(3):336-343

Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, China.

Objective: To explore the effect of estradiol (E2) binding to its receptor ERβ on the proliferation and apoptosis of C28I2 cells.

Objective: We cloned the sequence of ESR2 into a recombinant adenovirus plasmid (pAd-ESR2) and packaged the plasmid in HEK293 cells. Normal human chondrocyte C28I2 cells were transfected with Ad-ESR2 or small interfering RNA targeting ESR2-siRNA (ESR2-siRNA), and the effects of treatment with DMSO or E2 on the expression of the proteins associated with endoplasmic reticulum (ER) stress and cell apoptosis were determined using Western blotting. Read More

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Serum resolvin E1 levels and its relationship with thyroid autoimmunity in Hashimoto's thyroiditis: a preliminary study.

BMC Endocr Disord 2021 Apr 13;21(1):66. Epub 2021 Apr 13.

Beijing Key Laboratory of Diabetes Research and Care, Center for Endocrine Metabolism and Immune Diseases, Lu He Hospital Capital Medical University, Beijing, China.

Background: Omega-3 polyunsaturated fatty acids (PUFAs) produce lipid mediators with both anti-inflammatory and pro-resolution properties, including resolvins. The purpose of this study was to detect serum resolvin E1 (RVE1) levels in Hashimoto's thyroiditis (HT) patients and healthy controls (HCs) and to evaluate the relationship of RVE1 with thyroid autoimmunity.

Methods: A total of 57 participants were recruited, including 30 untreated HT patients and 27 age- and sex-matched HCs. Read More

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Screening for inborn errors of metabolism in psychotic patients using Next Generation Sequencing.

J Psychiatr Res 2021 Apr 1;138:125-129. Epub 2021 Apr 1.

Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Faculty of Health and Life Sciences (FHML), Maastricht University, Maastricht, the Netherlands.

Inborn errors of metabolism (IEMs) are a group of rare genetic disorders which, when emerging later in life, are often characterized by neuropsychiatric manifestations including psychosis. This study aimed to determine whether it would be useful to screen patients presenting with a psychotic disorder for IEMs by a single blood sample using Next Generation Sequencing (NGS), in order to detect rare, treatable causes of psychotic disorders. Blood was drawn from 60 patients with a psychotic disorder, with a duration of illness of less than 5 years. Read More

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Urea biosensors: A comprehensive review.

Biotechnol Appl Biochem 2021 Apr 13. Epub 2021 Apr 13.

Department of Physics, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India.

Present study is specially designed for the recent advances in biosensors to detect and quantify urea concentration. Urea (carbamide) is an organic compound made up of the carbonyl (C=O) functional group with two -NH groups having chemical formula CO (NH ) . In nature, urea is found everywhere as the result of various processes, and in the human body, urea is an end product of nitrogen metabolism. Read More

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miR‑654‑5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration.

Mol Med Rep 2021 Jun 13;23(6). Epub 2021 Apr 13.

Department of Orthopaedics, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu 210009, P.R. China.

Intervertebral disc degeneration (IDD) is a common chronic disease characterized by the loss of extracellular matrix (ECM) in the nucleus pulposus (NP). Accumulating evidence has revealed that abnormal expression of microRNAs (miRs) is closely associated with IDD development. The present study aimed to investigate the precise role and possible mechanism underlying the effects of miR‑654‑5p in the pathogenesis of IDD. Read More

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MicroRNA-200b-3p promotes endothelial cell apoptosis by targeting HDAC4 in atherosclerosis.

BMC Cardiovasc Disord 2021 Apr 12;21(1):172. Epub 2021 Apr 12.

Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Background: Epicardial adipose tissue (EAT) shares the same microcirculation with coronary arteries through coronary arteries branches, and contributes to the development of atherosclerosis. MicroRNAs (miRNAs) are involved in the formation of atherosclerosis. However, the alteration of miRNA profile in EAT during atherosclerosis is still uncovered. Read More

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A Coumarin-Porphyrin FRET Break-Apart Probe for Heme Oxygenase-1.

J Am Chem Soc 2021 Apr 12. Epub 2021 Apr 12.

Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, Wood Lane, London W12 0BZ, U.K.

Heme oxygenase-1 (HO-1) is a vital enzyme in humans that primarily regulates free heme concentrations. The overexpression of HO-1 is commonly associated with cardiovascular and neurodegenerative diseases including atherosclerosis and ischemic stroke. Currently, there are no known chemical probes to detect HO-1 activity, limiting its potential as an early diagnostic/prognostic marker in these serious diseases. Read More

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