2,569 results match your criteria dermatitis technique

Enzymatic recombinase amplification coupled with CRISPR-Cas12a for ultrasensitive, rapid, and specific Porcine circovirus 3 detection.

Mol Cell Probes 2021 Sep 9;59:101763. Epub 2021 Sep 9.

Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Shushan District West Yangtze River Road 130#, Hefei, 230036, Anhui Province, People's Republic of China. Electronic address:

Porcine circovirus type 3 (PCV3) is a disease associated with porcine dermatitis and nephrotic syndrome (PDNS) that has caused significant economic losses to swine herds since its discovery in 2016. To develop a simple, on-site, rapid, and sensitive assay to combat the spread of PCV3, we optimized the CRISPR/Cas12a (also known as Cpf1) system combined with enzymatic recombinase amplification (ERA) nucleic acid amplification to diagnose PCV3. The results showed that the ERA-CRISPR/Cas12a reaction could detect PCV3 within 1 h in genomic DNA harboring a minimum of seven copies. Read More

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September 2021

Deep Learning-Based High-Frequency Ultrasound Skin Image Classification with Multicriteria Model Evaluation.

Sensors (Basel) 2021 Aug 30;21(17). Epub 2021 Aug 30.

Department of Dermatology, Military Institute of Medicine, 01-755 Warszawa, Poland.

This study presents the first application of convolutional neural networks to high-frequency ultrasound skin image classification. This type of imaging opens up new opportunities in dermatology, showing inflammatory diseases such as atopic dermatitis, psoriasis, or skin lesions. We collected a database of 631 images with healthy skin and different skin pathologies to train and assess all stages of the methodology. Read More

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Tacrolimus Encapsulated Mesoporous Silica Nanoparticles Embedded Hydrogel for the Treatment of Atopic Dermatitis.

Int J Pharm 2021 Sep 6:121079. Epub 2021 Sep 6.

Institute of Pharmacy, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad - 382481, Gujarat, India. Electronic address:

Atopic dermatitis (AD) is a repetitive inflammatory skin disorder with limited treatment options. Innovative targeted therapies are gaining significant interest and momentum towards disease control including better ways to deliver drugs topically. Tacrolimus is one such compound which is used to manage moderate to severe AD without causing atrophy which is one of the common side effects of steroids. Read More

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September 2021

Dermoscopic Evaluation of Inflammatory Nail Disorders and Their Mimics.

Acta Derm Venereol 2021 Sep 15;101(9):adv00548. Epub 2021 Sep 15.

Alfred Health, Melbourne, Australia.

Nail dermoscopy (onychoscopy) is a valuable diagnostic tool for evaluating diseases in the nail apparatus. It is non-invasive, allowing clinicians to prioritize particular nails for biopsy. Thus, it can improve diagnostic accuracy and expedite treatment. Read More

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September 2021

Molecular detection of Strongyloides sp. in Australian Thoroughbred foals.

Parasit Vectors 2021 Sep 3;14(1):444. Epub 2021 Sep 3.

Melbourne Veterinary School, The University of Melbourne, Werribee, Australia.

Background: Strongyloides westeri is found in the small intestine of young horses, mainly in foals up to about 16 weeks of age. The main source of infection for foals is through transmammary transmission, and foals can develop acute diarrhoea, weakness, dermatitis and respiratory signs. The epidemiology of S. Read More

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September 2021

[Patient selection and early tolerance of whole breast irradiation according to the "Fast Forward" protocol: Preliminary results].

Cancer Radiother 2021 Aug 25. Epub 2021 Aug 25.

Département d'oncologie radiothérapie, institut Curie, 75005 Paris, France. Electronic address:

Purpose: Moderate hypofractionated radiotherapy has become routine practice for a selected population of patients treated for early-stage breast cancer. In April 2020, the Fast Forward (FF) study was published which introduced another extreme hypofractionated radiotherapy regimen in five sessions over a week. The aim of this work is to evaluate the population of first patients in whom this regimen was used in our department, as well as the results in terms of early toxicity. Read More

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Effects of chloroform fraction of Fritillariae Thunbergii Bulbus on atopic symptoms in a DNCB-induced atopic dermatitis-like skin lesion model and in vitro models.

J Ethnopharmacol 2021 Jul 24;281:114453. Epub 2021 Jul 24.

Department of Anatomy, College of Korean Medicine, Kyung Hee University, 26, Kyunghee dae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea. Electronic address:

Ethnopharmacological Relevance: Fritillariae thunbergii Bulbus (FT), knowns as "Jeolpaemo ()" in Korean traditional medicine, is a perennial plant belonging to the Liliaceae family and has been used to treat symptoms such as cough, sputum formation, and purulent pneumonia. Owing to its effects of lowering heat, removing sputum, and reducing swelling, the plant has also been used as an external prescription medicine to treat inflammation.

Aim Of The Study: To analyze the anti-inflammatory effects of FT-ethanol extract (FT-Et) and FT-chloroform fraction extract (FT-Cl) on 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD) in vivo and in vitro. Read More

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Atopic dermatitis trajectories to age 8 years in the GUSTO cohort.

Clin Exp Allergy 2021 Sep 31;51(9):1195-1206. Epub 2021 Jul 31.

Singapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

Background: The heterogeneity of childhood atopic dermatitis (AD) underscores the need to understand latent phenotypes that may inform risk stratification and disease prognostication.

Objective: To identify AD trajectories across the first 8 years of life and investigate risk factors associated with each trajectory and their relationships with other comorbidities.

Methods: Data were collected prospectively from 1152 mother-offspring dyads in the Growing Up in Singapore Towards healthy Outcomes (GUSTO) cohort from ages 3 months to 8 years. Read More

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September 2021

The 10-Point Plan 2021: Updated Concepts for Improved Procedural Safety During Facial Filler Treatments.

Clin Cosmet Investig Dermatol 2021 6;14:779-814. Epub 2021 Jul 6.

Maxillofacial Department, University of Verona, Verona, Italy.

Dermal filler treatments require constant reassessment for improving and safeguarding the rapidly evolving aesthetic field. Suboptimal injection technique, patient selection and product knowledge have touted a concerning increase in filler complications, with new challenges such as the COVID-19 pandemic leading to new paradigms in the understanding, prevention, diagnosis and treatment of complications. The updated 10-point plan has been developed to curtail complications through consideration of causative factors, categorized as patient, product, and procedure-related. Read More

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Acquired perforating dermatoses show increased levels of cutaneous advanced glycation end-products.

Clin Exp Dermatol 2021 Jul 14. Epub 2021 Jul 14.

Section of Dermatology and Venereology, Department of Medicine, University of Verona, Verona, Italy.

Background: Acquired perforating dermatoses (APDs) are characterized by transepidermal elimination of skin materials. Altered glycation of dermal components may be involved in pathogenesis.

Aim: To assess whether patients affected by APDs have increased levels of cutaneous advanced glycation end-products (AGEs). Read More

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Contact Dermatitis: Overcoming Challenges of Specific Patients, Deciphering the Results and Reaching a Correct Diagnosis.

Handb Exp Pharmacol 2021 Jul 9. Epub 2021 Jul 9.

Department of Dermatology, Hospital del Mar, Institut Mar d´Investigacions Mèdiques, Universitat Autònoma, Barcelona, Spain.

Skin lesions caused by allergic contact dermatitis are an important occupational and environmental disease. Patch testing is the gold-standard procedure used to diagnose allergic contact dermatitis.The present chapter summarizes aspects of patch testing for the diagnosis of contact allergy: important working definitions, relevance of treating contact dermatitis, materials, technique, test result and interpretation, and special consideration regarding individual factors which influence the patch test outcome or necessitate special attention. Read More

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Early Results of Popliteal Vein External Banding for Treatment of Chronic Venous Insufficiency.

Ann Vasc Surg 2021 Jun 18. Epub 2021 Jun 18.

Department of Surgery, Division of Vascular Surgery, Emory University Hospital, Atlanta, GA; Department of Surgery, Division of Vascular Surgery, Grady Health, Atlanta, GA. Electronic address:

Background: Chronic venous insufficiency (CVI) encompasses a myriad of clinical manifestations including lower extremity swelling and pain, ulcerations and chronic skin changes such as stasis dermatitis, and lipodermatosclerosis. CVI effects greater than 25 million Americans and has a significant socioeconomic and psychosocial impact. Treatment of CVI varies depending on the etiology. Read More

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Pathology of female mice experimentally infected with an in vitro cultured strain of Trypanosoma equiperdum.

J Vet Med Sci 2021 Aug 16;83(8):1212-1218. Epub 2021 Jun 16.

Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.

Dourine, caused by infection with Trypanosoma equiperdum, is one of the trypanosomiasis in equids. The clinical course of dourine is long-term, ranging from 1-2 months to several years. Since the pathogenesis of dourine has not yet been elucidated, experimental studies using mouse infection models are needed. Read More

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Recommendations for Vaccination in Children with Atopic Dermatitis Treated with Dupilumab: A Consensus Meeting, 2020.

Am J Clin Dermatol 2021 Jul 2;22(4):443-455. Epub 2021 Jun 2.

Section of Community Pediatrics, Department of Pediatrics, Alberta Children's Hospital and University of Calgary, Calgary, AB, Canada.

Dupilumab is the only biologic therapy currently approved in Europe and the United States for severe atopic dermatitis in patients 6 years of age or older. Off-label use is rationalized in younger children with severe atopic dermatitis. Decisions about vaccination for children on dupilumab are complex and depend on both the child's current treatment and the type of vaccination required. Read More

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Case Report: Extensive Dermatitis Secondary to Severe Malnutrition, Zinc and Vitamin Deficiencies After Malabsorptive Bariatric Surgery.

Front Endocrinol (Lausanne) 2021 12;12:623543. Epub 2021 May 12.

Endocrinology and Nutrition Service, Reina Sofía University Hospital, Córdoba, Spain.

Bariatric surgery is one of the most effective treatments currently available for obesity and its derived comorbidities. However, complications may occur, especially when malabsorptive surgeries like a biliopancreatic diversion is performed. We present the case of a female patient whose obesity was treated with this technique, and in the 9 year of follow-up developed an extensive dermatitis secondary to zinc deficiency and malnutrition, precipitated by therapeutic non-compliance. Read More

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Combined internal and external negative pressure wound therapy: breakthrough treatment for lymphocutaneous intractable fistula.

Surg Today 2021 May 16. Epub 2021 May 16.

Department of Plastic and Reconstructive Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan.

Purpose: Lymphocutaneous fistula after lymph node dissection is intractable, yet there is no established treatment strategy. This study demonstrates the wound closure time achieved by a new method of combined internal and external negative pressure wound therapy (CIEN) in patients with lymphocutaneous fistula.

Methods: The subjects of this study were six consecutive patients with lymphocutaneous fistula after lymphatic surgery, who were treated with CIEN between 2018 and 2020. Read More

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A novel technique for superficial complicated stomas using the Dacron vascular prosthesis.

Langenbecks Arch Surg 2021 Jun 14;406(4):1239-1244. Epub 2021 May 14.

Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, 14048-900, Brazil.

Background And Purpose: Despite the advances achieved in surgical techniques in recent years, an intestinal stoma is still needed in many patients undergoing colorectal surgery. However, the intestinal stoma may be associated with serious complications and the need for a second surgical procedure. In extreme cases, when it is not possible to access the abdominal cavity, the management of a complicated stoma is challenging. Read More

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Initial Experience of Intentional Internal High-Dose Policy Volumetric Modulated Arc Therapy of Neck Lymph Node Metastases ≥ 2 cm in Patients With Head and Neck Squamous Cell Carcinoma.

Front Oncol 2021 27;11:651409. Epub 2021 Apr 27.

Department of Radiation Therapy in National Cancer Center Hospital, Tokyo, Japan.

Background And Purpose: Most locoregional recurrences after definitive radiotherapy for head and neck squamous cell carcinoma (HNSCC) develop "in-field." Dose escalation while sparing organs at risk can be a good solution for improving local control without increasing adverse effects. This study investigated the safety and effectiveness of volumetric modulated arc therapy (VMAT) using intentionally internal high-dose policy (IIHDP) to treat neck lymph node metastases (NLNM) ≥ 2 cm in HNSCC patients. Read More

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Chronological Skin Changes Through Postmastectomy Radiotherapy Based on Radiotherapy Techniques: Using Quantitative Dermatological Measurements.

Skin Pharmacol Physiol 2021 7;34(4):229-238. Epub 2021 May 7.

Skin Research Institute, AMOREPACIFIC Corporation R&D Center, Youngin, Republic of Korea.

Background: There has been few research on how to measure skin status quantitatively throughout the course of radiotherapy (RT). We evaluated the changes in the skin induced by 2 different RT techniques using objective measurements in breast cancer patients.

Methods: In this prospective study, between August 2015 and March 2019, serial measurements of the dermatological factors during and after postmastectomy radiotherapy (PMRT) were made in 40 breast cancer patients. Read More

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Identification of Novel Toxin Genes from the Stinging Nettle Caterpillar (Cramer, 1799): Insights into the Evolution of Lepidoptera Toxins.

Insects 2021 Apr 29;12(5). Epub 2021 Apr 29.

Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.

Many animal species can produce venom for defense, predation, and competition. The venom usually contains diverse peptide and protein toxins, including neurotoxins, proteolytic enzymes, protease inhibitors, and allergens. Some drugs for cancer, neurological disorders, and analgesics were developed based on animal toxin structures and functions. Read More

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Application of Cellular Resolution Full-Field Optical Coherence Tomography in vivo for the Diagnosis of Skin Tumours and Inflammatory Skin Diseases: A Pilot Study.

Dermatology 2021 May 4:1-11. Epub 2021 May 4.

Department of Dermatology, MacKay Memorial Hospital, Taipei, Taiwan.

Background: Optical coherence tomography (OCT) has been shown to provide non-invasive diagnosis of common skin neoplasms, especially basal cell carcinoma. OCT produces a cross-sectional view of the tissue, similar to a traditionally sectioned histopathological view, but the resolution of conventional OCT is low and thus limits clinical application.

Objectives: This study aimed to investigate the application ability of a full-field (FF)OCT system which was newly developed to scan the skin at the cellular level. Read More

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It is no skin off my nose: The relationship between the skin and allergic rhinitis.

Ann Allergy Asthma Immunol 2021 08 23;127(2):176-182. Epub 2021 Apr 23.

School of Medicine, Queen's University, Kingston, Ontario, Canada; Division of Dermatology, Department of Medicine, Queen's University, Kingston, Ontario, Canada. Electronic address:

Objective: Evidence supports the relationship between the skin barrier and allergic conditions. This narrative review evaluates what role the cutaneous barrier may play in the pathogenesis, disease course, and management of allergic rhinitis (AR).

Data Sources: A literature review of the MEDLINE (PubMed), Embase, Cochrane, and SCOPUS Sciverse databases was conducted to identify available evidence. Read More

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Direct assessment of individual skin barrier components by electrical impedance spectroscopy.

Allergy 2021 Apr 12. Epub 2021 Apr 12.

Institute of Clinical Physiology/Nutritional Medicine, Medical Department, Division of Gastroenterology, Infectiology, Rheumatology, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Background: Expression of the tight junction proteins Cldn1 and 4 is altered in skin diseases such as atopic dermatitis, and Cldn1 deficiency affects skin barrier formation. Impedance spectroscopy (IS) has been proven to allow detection of alterations in the skin barrier but is currently unable to separate effects on viable epidermis (VE) and stratum corneum (SC).

Methods: Effects of siRNA-mediated Cldn1 and 4 knockdown in reconstructed human epidermis (RHE) on VE and SC barrier function were investigated with Ussing chamber-based IS. Read More

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Radiation-induced acute injury of intensity-modulated radiotherapy versus three-dimensional conformal radiotherapy in induction chemotherapy followed by concurrent chemoradiotherapy for locoregionally advanced nasopharyngeal carcinoma: a prospective cohort study.

Sci Rep 2021 Apr 8;11(1):7693. Epub 2021 Apr 8.

School of Public Health, Guangdong Pharmaceutical University, No. 283 Jianghai Avenue, Haizhu District, Guangzhou, 510310, China.

To address whether the addition of intensity-modulated radiotherapy (IMRT) compared to three-dimensional conformal radiotherapy (3D-CRT) aggravate radiation-induced acute injury of locoregionally advanced nasopharyngeal carcinoma (LANPC) patients with induction chemotherapy (IC) followed by concurrent chemoradiotherapy (CCRT). We conducted a prospective study of 182 patients in the stage III to IVb with biopsy-proven nonmetastatic LANPC who newly underwent radiotherapy and sequentially received IC, followed by CCRT at our institution. Occurring time of radiation-induced toxicities were estimated and compared using the Kaplan-Meier method and Log-rank test. Read More

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Screening of Novel Source for Genistein by Rapid and Sensitive UPLC-APCI-TOF Mass Spectrometry.

Int J Food Sci 2021 16;2021:5537917. Epub 2021 Mar 16.

Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, JSS TI Campus, Mysuru, Karnataka 57006, India.

Genistein has been shown to have a broad spectrum of health advantages. Only legumes were reported to have a significant amount of genistein with the highest concentration in Soybean. Soybean was found to cause allergies in children with atopic dermatitis and in adults. Read More

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Methylene blue-mediated antimicrobial photodynamic therapy can be a novel non-antibiotic platform for bovine digital dermatitis.

Photodiagnosis Photodyn Ther 2021 Jun 31;34:102274. Epub 2021 Mar 31.

Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.

Background: Bovine digital dermatitis (BDD) is one of the most important diseases that effect dairy cows. Methylene blue-mediated antimicrobial photodynamic therapy (MB-APDT) emerges as a promising technique to treat superficial infections in bovines.

Methods: Twenty BDD lesions located at the skin horn transition of the claw of pelvic limbs of 16 cows were treated by MB-APDT, using a red LED cluster (λ = 660 nm, irradiance =60 mW/cm, exposure time = 40 s) combined with topical application of MB at 0. Read More

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A randomized prospective study comparing acute toxicity, compliance and objective response rate between simultaneous integrated boost and sequential intensity-modulated radiotherapy for locally advanced head and neck cancer.

Radiat Oncol J 2021 Mar 18;39(1):15-23. Epub 2021 Mar 18.

Department of Radiotherapy, S.M.S Medical College and Hospital, Jaipur, Rajasthan, India.

Purpose: Intensity-modulated radiotherapy (IMRT) provides higher dose to target volumes and limits the dose to normal tissues. IMRT may be applied using either simultaneous integrated boost (SIB-IMRT) or sequential boost (SEQ-IMRT) technique. The objectives of this study were to compare acute toxicity and objective response rates between SIB-IMRT and SEQ-IMRT in patients with locally advanced head and neck cancer. Read More

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Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models.

Sci Rep 2021 03 18;11(1):6217. Epub 2021 Mar 18.

L'Oréal Research and Innovation, Aulnay-sous-Bois, France.

Organotypic skin tissue models have decades of use for basic research applications, the treatment of burns, and for efficacy/safety evaluation studies. The complex and heterogeneous nature of native human skin however creates difficulties for the construction of physiologically comparable organotypic models. Within the present study, we utilized bioprinting technology for the controlled deposition of separate keratinocyte subpopulations to create a reconstructed epidermis with two distinct halves in a single insert, each comprised of a different keratinocyte sub-population, in order to better model heterogonous skin and reduce inter-sample variability. Read More

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A beginner's guide to mucous membrane grafting for lid margin keratinization: Review of indications, surgical technique and clinical outcomes.

Indian J Ophthalmol 2021 04 28;69(4):794-805. Epub 2021 Jan 28.

The Cornea Institute; Center for Ocular Regeneration (CORE); Brien Holden Eye Research Centre, L V Prasad Eye Institute, Hyderabad, Telangana, India.

The posterior lid margin, where the mucocutaneous junction (MCJ) between the eyelid skin and tarsal conjunctiva is located, plays a critical role in maintaining the homeostasis of the ocular surface. Posterior migration of the MCJ leads to lid-margin keratinization (LMK), which has a domino effect on the delicate balance of the ocular surface microenvironment. This occurs most commonly following Stevens-Johnson syndrome/toxic epidermal necrolysis and is not known to regress spontaneously or with medical therapy. Read More

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"Christie Regimen" palliative radiotherapy in advanced head-and-neck cancer: A single-center experience.

J Cancer Res Ther 2021 Jan-Mar;17(1):88-93

Department of Radiation Oncology, Lok Nayak Hospital and Associated Maulana Azad Medical College, New Delhi, India.

Background: Relevance of aggressive treatment in advanced head neck squamous cell cancers(HNSCC) is debatable in view of expected poor outcome. Long treatment duration only adds up to the cost of treatment without any improvements in outcomes.

Aims And Objectives: To assess the outcomes of hypofractionated "Christie" palliative radiotherapy regimen in advanced HNSCC. Read More

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