10,066 results match your criteria derived retinal


7, 8-Dihydroxyflavone, a TrkB receptor agonist, provides minimal protection against retinal vascular damage during oxygen-induced ischemic retinopathy.

PLoS One 2021 2;16(12):e0260793. Epub 2021 Dec 2.

Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States of America.

Retinopathy of prematurity (ROP) is one of the main causes of blindness in children worldwide. Brain-derived neurotrophic factor (BDNF) and its receptor, tropomyosin-related kinase B (TrkB), play critical protective roles in the development and function of neurons and vasculature. Lack of BDNF expression results in increased endothelial cell apoptosis and reduced endothelial cell-cell contact. Read More

View Article and Full-Text PDF
December 2021

Involvement of Gap Junctions in Acetylcholine-Induced Endothelium-Derived Hyperpolarization-Type Dilation of Retinal Arterioles in Rats.

Biol Pharm Bull 2021 ;44(12):1860-1865

Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences.

An electrical communication between the endothelial and smooth muscle cells via gap junctions, which provides the signaling pathway known as endothelium-dependent hyperpolarization (EDH), plays a crucial role in controlling the vascular tone. In this study, we investigated the role of gap junctions in the acetylcholine (ACh)-induced EDH-type dilation of rat retinal arterioles in vivo. The dilator response was evaluated by measuring the diameter of retinal arterioles. Read More

View Article and Full-Text PDF
January 2021

Altered H3 histone acetylation impairs high-fidelity DNA repair to promote cerebellar degeneration in spinocerebellar ataxia type 7.

Cell Rep 2021 Nov;37(9):110062

Departments of Pathology & Laboratory Medicine, Neurology, and Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA; Department of Neurology, Duke University School of Medicine, Durham, NC 27710, USA; UCI Institute for Neurotherapeutics, University of California, Irvine, Irvine, CA 92697, USA. Electronic address:

A common mechanism in inherited ataxia is a vulnerability of DNA damage. Spinocerebellar ataxia type 7 (SCA7) is a CAG-polyglutamine-repeat disorder characterized by cerebellar and retinal degeneration. Polyglutamine-expanded ataxin-7 protein incorporates into STAGA co-activator complex and interferes with transcription by altering histone acetylation. Read More

View Article and Full-Text PDF
November 2021

Spectral fundus autofluorescence peak emission wavelength in ageing and AMD.

Acta Ophthalmol 2021 Dec 1. Epub 2021 Dec 1.

Department of Ophthalmology, University Hospital Jena, Jena, Germany.

Purpose: To investigate the spectral characteristics of fundus autofluorescence (FAF) in AMD patients and controls.

Methods: Fundus autofluorescence spectral characteristics was described by the peak emission wavelength (PEW) of the spectra. Peak emission wavelength (PEW) was derived from the ratio of FAF recordings in two spectral channels at 500-560 nm and 560-720 nm by fluorescence lifetime imaging ophthalmoscopy. Read More

View Article and Full-Text PDF
December 2021

Selective retina therapy and thermal stimulation of the retina: different regenerative properties - implications for AMD therapy.

BMC Ophthalmol 2021 Nov 30;21(1):412. Epub 2021 Nov 30.

Department of Ophthalmology, Christian-Albrechts-University of Kiel, University Medical Center, Kiel, Germany.

Background: Selective Retina Therapy (SRT), a photodisruptive micropulsed laser modality that selectively destroys RPE cells followed by regeneration, and Thermal Stimulation of the Retina (TSR), a stimulative photothermal continuous wave laser modality that leads to an instant sublethal temperature increase in RPE cells, have shown therapeutic effects on Age-related Macular Degeneration (AMD) in mice. We investigate the differences between both laser modalities concerning RPE regeneration.

Methods: For PCR array, 6 eyes of murine AMD models, apolipoprotein E and nuclear factor erythroid-derived 2- like 2 knock out mice respectively, were treated by neuroretina-sparing TSR or SRT. Read More

View Article and Full-Text PDF
November 2021

Pattern Electroretinogram Parameters Are Associated with Optic Nerve Morphology in Preperimetric Glaucoma after Adjusting for Disc Area.

J Ophthalmol 2021 13;2021:8025337. Epub 2021 Oct 13.

Manhattan Eye Ear and Throat Hospital, New York, NY 10065, USA.

Purpose: We examined the relationships between pattern electroretinogram and optical coherence tomography derived optic nerve head measurements, after controlling for disc area.

Methods: Thirty-two eyes from 20 subjects with preperimetric glaucoma underwent pattern electroretinogram and optical coherence tomography. Pattern electroretinogram parameters (Magnitude, MagnitudeD, and MagnitudeD/Magnitude ratio) and optic nerve head measurements (rim area, average cup to disc ratio, vertical cup to disc ratio, cup volume, retinal nerve fiber layer thickness sectors, and Bruch's membrane opening-minimum rim width thickness sectors) were analyzed after controlling for disc area. Read More

View Article and Full-Text PDF
October 2021

Animal Models of LED-Induced Phototoxicity. Short- and Long-Term In Vivo and Ex Vivo Retinal Alterations.

Life (Basel) 2021 Oct 26;11(11). Epub 2021 Oct 26.

Departamento de Oftalmología, Universidad de Murcia e Instituto Murciano de Investigación Biosanitaria (IMIB) Virgen de la Arrixaca, Campus de CC de la Salud, El Palmar, 30120 Murcia, Spain.

Phototoxicity animal models have been largely studied due to their degenerative communalities with human pathologies, e.g., age-related macular degeneration (AMD). Read More

View Article and Full-Text PDF
October 2021

Mesenchymal Stem Cell Induced Foxp3(+) Tregs Suppress Effector T Cells and Protect against Retinal Ischemic Injury.

Cells 2021 Nov 4;10(11). Epub 2021 Nov 4.

Department of Ophthalmology, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

Mesenchymal stem/stromal cells (MSC) are well known for immunomodulation; however, the mechanisms involved in their benefits in the ischemic retina are unknown. This study tested the hypothesis that MSC induces upregulation of transcription factor forkhead box protein P3 (Foxp3) in T cells to elicit immune modulation, and thus, protect against retinal damage. Induced MSCs (iMSCs) were generated by differentiating the induced pluripotent stem cells (iPSC) derived from urinary epithelial cells through a noninsertional reprogramming approach. Read More

View Article and Full-Text PDF
November 2021

Long-Term Transplant Effects of iPSC-RPE Monolayer in Immunodeficient RCS Rats.

Cells 2021 Oct 29;10(11). Epub 2021 Oct 29.

Department of Ophthalmology, Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.

Retinal pigment epithelium (RPE) replacement therapy is evolving as a feasible approach to treat age-related macular degeneration (AMD). In many preclinical studies, RPE cells are transplanted as a cell suspension into immunosuppressed animal eyes and transplant effects have been monitored only short-term. We investigated the long-term effects of human Induced pluripotent stem-cell-derived RPE (iPSC-RPE) transplants in an immunodeficient Royal College of Surgeons (RCS) rat model, in which RPE dysfunction led to photoreceptor degeneration. Read More

View Article and Full-Text PDF
October 2021

A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues.

Cells 2021 Oct 26;10(11). Epub 2021 Oct 26.

Department of Immunology, Faculty and Graduate School of Medicine, Mie University, Tsu 514-8507, Mie, Japan.

Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cells. Read More

View Article and Full-Text PDF
October 2021

Detection of Mycoplasma Contamination in Transplanted Retinal Cells by Rapid and Sensitive Polymerase Chain Reaction Test.

Int J Mol Sci 2021 Nov 21;22(22). Epub 2021 Nov 21.

RIKEN Center for Biosystems Dynamics Research, Laboratory for Retinal Regeneration, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan.

Contamination of cells/tissues by infectious pathogens (e.g., fungi, viruses, or bacteria, including mycoplasma) is a major problem in cell-based transplantation. Read More

View Article and Full-Text PDF
November 2021

Human Pluripotent Stem Cell-Derived Neural Progenitor Cells Promote Retinal Ganglion Cell Survival and Axon Recovery in an Optic Nerve Compression Animal Model.

Int J Mol Sci 2021 Nov 20;22(22). Epub 2021 Nov 20.

Department of Ophthalmology, CHA Bundang Medical Center, CHA University, Seongnam 13496, Gyeonggi-do, Korea.

Human pluripotent stem cell-derived neural progenitor cells (NPCs) have the potential to recover from nerve injury. We previously reported that human placenta-derived mesenchymal stem cells (PSCs) have neuroprotective effects. To evaluate the potential benefit of NPCs, we compared them to PSCs using R28 cells under hypoxic conditions and a rat model of optic nerve injury. Read More

View Article and Full-Text PDF
November 2021

Complement Factor H Loss in RPE Cells Causes Retinal Degeneration in a Human RPE-Porcine Retinal Explant Co-Culture Model.

Biomolecules 2021 Nov 3;11(11). Epub 2021 Nov 3.

Institute for Ophthalmic Research, Department for Ophthalmology, Eberhard Karls University of Tübingen, 72076 Tübingen, Germany.

Age-related Macular degeneration (AMD) is a degenerative disease of the macula affecting the elderly population. Treatment options are limited, partly due to the lack of understanding of AMD pathology and the lack of suitable research models that replicate the complexity of the human macula and the intricate interplay of the genetic, aging and lifestyle risk factors contributing to AMD. One of the main genetic risks associated with AMD is located on the Complement Factor H () gene, leading to an amino acid substitution in the Factor H (FH) protein (Y402H). Read More

View Article and Full-Text PDF
November 2021

Deletion of transcription factor AP-2β from the developing murine trabecular meshwork region leads to progressive glaucomatous changes.

J Neurosci Res 2021 Nov 25. Epub 2021 Nov 25.

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.

Glaucoma is one of the leading causes of irreversible blindness and can result from abnormalities in anterior segment structures required for aqueous humor outflow, including the trabecular meshwork (TM) and Schlemm's canal (SC). Transcription factors such as AP-2β play critical roles in anterior segment development. Here, we show that the Mgp-Cre knock-in (Mgp-Cre. Read More

View Article and Full-Text PDF
November 2021

'The role of accommodative function in myopic development: A review.'

Semin Ophthalmol 2021 Nov 25:1-7. Epub 2021 Nov 25.

2 Department of Ophthalmology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.

Involvement of the accommodative mechanism in myopia progression has been hypothesised and investigated over the past years, given the emerging myopia crisis across the globe. This review aimed at exploring the clinical role of accommodative function in myopia development. A literature search of MEDLINE and EMBASE was performed from conception to May 2021. Read More

View Article and Full-Text PDF
November 2021

Embryonic pericytes promote microglial homeostasis and their effects on neural progenitors in the developing cerebral cortex.

J Neurosci 2021 Nov 24. Epub 2021 Nov 24.

Department of Anatomy and Cell Biology, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.

Multifaceted microglial functions in the developing brain, such as promoting the differentiation of neural progenitors and contributing to the positioning and survival of neurons, have been progressively revealed. Although previous studies have noted the relationship between vascular endothelial cells and microglia in the developing brain, little attention has been given to the importance of pericytes, the mural cells surrounding endothelial cells. In this study, we attempted to dissect the role of pericytes in microglial distribution and function in developing mouse brains. Read More

View Article and Full-Text PDF
November 2021

Single-Examination Risk Prediction of Severe Retinopathy of Prematurity.

Pediatrics 2021 Dec;148(6)

Departments of Ophthalmology.

Background And Objectives: Retinopathy of prematurity (ROP) is a leading cause of childhood blindness. Screening and treatment reduces this risk, but requires multiple examinations of infants, most of whom will not develop severe disease. Previous work has suggested that artificial intelligence may be able to detect incident severe disease (treatment-requiring retinopathy of prematurity [TR-ROP]) before clinical diagnosis. Read More

View Article and Full-Text PDF
December 2021

Human umbilical cord-derived mesenchymal stem cells treatment for refractory uveitis: a case series.

Int J Ophthalmol 2021 18;14(11):1784-1790. Epub 2021 Nov 18.

Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin 300384, China.

Aim: To evaluate therapeutic outcomes of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) treatment in patients with refractory uveitis.

Methods: A retrospective and noncomparative review was performed on four patients with refractory uveitis from December 2013 to December 2017. HUC-MSCs were administered intravenously at a dose of 1×10 cells/kg. Read More

View Article and Full-Text PDF
November 2021

Protective effect of human umbilical cord mesenchymal stem cell-derived exosomes on rat retinal neurons in hyperglycemia through the brain-derived neurotrophic factor/TrkB pathway.

Int J Ophthalmol 2021 18;14(11):1683-1689. Epub 2021 Nov 18.

College of Medicine, Nankai University, Tianjin 300071, China.

Aim: To explore whether human umbilical cord mesenchymal stem cell (hUCMSC)-derived exosomes (hUCMSC-Exos) protect rat retinal neurons in high-glucose (HG) conditions by activating the brain-derived neurotrophic factor (BDNF)-TrkB pathway.

Methods: hUCMSC-Exos were collected with differential ultracentrifugation methods and observed by transmission electron microscopy. Enzyme-linked immunosorbent assays (ELISAs) was used to quantify BDNF in hUCMSC-Exos, and Western blot was used to identify surface markers of hUCMSC-Exos. Read More

View Article and Full-Text PDF
November 2021

Microglia-derived TNF-α mediates Müller cell activation by activating the TNFR1-NF-κB pathway.

Exp Eye Res 2021 Nov 18;214:108852. Epub 2021 Nov 18.

Eye Institute, Affiliated Hospital of Nantong University, Nantong, 226001, China. Electronic address:

Microglia and its interaction with Müller cells are responsible to retinal surveillance during retinal neurodegeneration, however, the role and mechanism of microglia-derived tumor necrosis factor (TNF)-α in the activation of retinal Müller cells have not been fully elucidated. In the present study, primary microglia and Müller cells were isolated from newborn Sprague-Dawley (SD) rats with purities of 88.2 ± 6. Read More

View Article and Full-Text PDF
November 2021

Generation of induced pluripotent stem cell (iPSC) line ZOCi003-A derived from peripheral blood mononuclear cells of X-linked juvenile retinoschisis harboring a hemizygous mutation in RS1 gene.

Stem Cell Res 2021 Nov 9;57:102595. Epub 2021 Nov 9.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China. Electronic address:

X-linked juvenile retinoschisis (XLRS) is an inherited disease characterized by splitting within inner retinal layers and impaired vision, which begins in childhood and occurs mostly in males. Peripheral blood mononuclear cells (PBMCs) were isolated from a seven-year-old boy carrying a hemizygous mutation in RS1 gene, and were reprogrammed into human induced pluripotent stem cells (iPSCs) using non-integrative episomal vectors. The cell line, ZOCi003-A, had normal karyotype, expressed pluripotency markers, and could differentiate into three germ layers in vivo. Read More

View Article and Full-Text PDF
November 2021

Therapeutic Effects of Melatonin on Ocular Diseases: Knowledge Map and Perspective.

Front Pharmacol 2021 2;12:721869. Epub 2021 Nov 2.

Department of Ophthalmology, Peking University Third Hospital, Beijing, China.

Melatonin plays a critical role in the pathophysiological process including circadian rhythm, apoptosis, and oxidative stress. It can be synthesized in ocular tissues, and its receptors are also found in the eye, triggering more investigations concentrated on the role of melatonin in the eye. In the past decades, the protective and therapeutic potentials of melatonin for ocular diseases have been widely revealed in animal models. Read More

View Article and Full-Text PDF
November 2021

Ganglion cell-inner plexiform layer measurements derived from widefield compared to montaged 9-field optical coherence tomography.

Clin Exp Optom 2021 Nov 18:1-9. Epub 2021 Nov 18.

School of Optometry and Vision Science, University of New South Wales (UNSW), Sydney, Australia.

Clinical Relevance: With equivalent inner retinal thickness measurements compared to a more conventional composite optical coherence tomography (OCT) protocol, Widefield optical coherence tomography (WF-OCT) is a clinically viable, time-saving option facilitating detection of ocular pathologies within the central 55° of the retina.

Purpose: To compare ganglion cell-inner plexiform layer (GCIPL) thicknesses obtained using a single WF-OCT scan and standard composite OCT scans acquired in 9 fields of gaze (9F-OCT).

Methods: Thirteen healthy participants underwent WF-OCT and 9F-OCT using the Spectralis OCT. Read More

View Article and Full-Text PDF
November 2021

Potential Protective Role of TRPM7 and Involvement of PKC/ERK Pathway in Blue Light-Induced Apoptosis in Retinal Pigment Epithelium Cells in Vitro.

Asia Pac J Ophthalmol (Phila) 2021 Nov 16. Epub 2021 Nov 16.

First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou City 350005, China.

Purpose: Blue light triggers apoptosis of retinal pigment epithelium (RPE) cells and causes retinal damage. The aim of this study was to elucidate the protective role of transient receptor potential melastatin 7 (TRPM7) in photodamaged RPE cells.

Methods: RPE cells were isolated from Sprague-Dawley (SD) rats and exposed to varying intensities of blue light (500-5000 lux) in vitro. Read More

View Article and Full-Text PDF
November 2021

The protective effects of pericyte-derived microvesicles on vascular endothelial functions via CTGF delivery in sepsis.

Cell Commun Signal 2021 Nov 16;19(1):115. Epub 2021 Nov 16.

State Key Laboratory of Trauma, Burns and Combined Injury, Shock and Transfusion Department, Army Medical Center of PLA, Daping Hospital, Army Medical University, No.10th Daping Changjiang Road, Chongqing, 400038, China.

Background: It is well known that sepsis is a prevalent severe disease caused by infection and the treatment strategies are limited. Recently pericyte-derived microvesicles (PMVs) were confirmed to be therapeutic in many diseases, whether PMVs can protect vascular endothelial cell (VEC) injury is unknown.

Methods: Pericytes were extracted from the retina of newly weaned rats, and PMVs were collected after starvation and characterized by flow-cytometry and transmission electron microscopy. Read More

View Article and Full-Text PDF
November 2021

Structure-function analysis for macular surgery in patients with coexisting glaucoma.

Graefes Arch Clin Exp Ophthalmol 2021 Nov 16. Epub 2021 Nov 16.

Department of Ophthalmology, Columbia University, New York, NY, USA.

Purpose: To develop methods to assess the effects of epiretinal membranes (ERM) and macular holes (MH) coexisting with glaucoma on pre-operative retinal structure and function and evaluate post-operative outcomes.

Methods: Seven eyes of 7 patients with glaucoma, 6 with ERMs and 1 with MH, were enrolled; 4 underwent vitrectomy for ERM and one for MH. Visual fields (VFs) and optical coherence tomography (OCT) scans were obtained pre- and post-operatively. Read More

View Article and Full-Text PDF
November 2021

Retinal regeneration requires dynamic Notch signaling.

Neural Regen Res 2022 Jun;17(6):1199-1209

Department of Biological Sciences, Center for Zebrafish Research, Center for Stem Cells and Regenerative Medicine, University of Notre Dame, Notre Dame, IN, USA.

Retinal damage in the adult zebrafish induces Müller glia reprogramming to produce neuronal progenitor cells that proliferate and differentiate into retinal neurons. Notch signaling, which is a fundamental mechanism known to drive cell-cell communication, is required to maintain Müller glia in a quiescent state in the undamaged retina, and repression of Notch signaling is necessary for Müller glia to reenter the cell cycle. The dynamic regulation of Notch signaling following retinal damage also directs proliferation and neurogenesis of the Müller glia-derived progenitor cells in a robust regeneration response. Read More

View Article and Full-Text PDF

Vitreous hemorrhage: A rare ophthalmic adverse effect due to imatinib treatment.

J Oncol Pharm Pract 2021 Nov 13:10781552211060730. Epub 2021 Nov 13.

University of Health Sciences, 146992Diskapi Yildirim Beyazit Training and Research Hospital, Department of Hematology, Ankara, Turkey.

Introduction: Imatinib is generally well tolerated by patients. The most common ophthalmic side effects are eyelid edema and periorbital edema. Other side effects which occur at rates of <1% include blepharitis, blurred vision, conjunctival hemorrhage, conjunctivitis, retinal hemorrhage, etc. Read More

View Article and Full-Text PDF
November 2021

The Role of RAD6B and PEDF in Retinal Degeneration.

Neuroscience 2021 Nov 11;480:19-31. Epub 2021 Nov 11.

Institute of Human Anatomy and Histoembryology, Basic Medical College, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, China. Electronic address:

RAD6B is an E2 ubiquitin-conjugating enzyme, playing an important role in DNA damage repair, gene expression, senescence, apoptosis and protein degradation. However, the specific mechanism between ubiquitin and retinal degeneration requires more investigation. Pigment epithelium-derived factor (PEDF) has a potent neurotrophic effect on the retina, protecting retinal neurons and photoreceptors from cell death caused by pathological damage. Read More

View Article and Full-Text PDF
November 2021

A Novel Method of Mouse RPE Explant Culture and Effective Introduction of Transgenes Using Adenoviral Transduction for Studies in AMD.

Int J Mol Sci 2021 Nov 5;22(21). Epub 2021 Nov 5.

Department of Ophthalmology, Children's Hospital of University of Pittsburgh School of Medicine, One Children's Hospital Drive, 4401 Penn Avenue, Pittsburgh, PA 15224, USA.

Degeneration of retinal pigment epithelium (RPE) is one of the most critical phenotypic changes of age-related macular degeneration (AMD), the leading cause of vision loss in the elderly. While cultured polarized RPE cells with original properties are valuable in models to study RPE biology and the consequences of genetic and/or pharmacological manipulations, the procedure to establish mouse primary PRE cell culture or pluripotent stem cell-derived RPE cells is time-consuming and yields a limited number of cells. Thus, establishing a mouse RPE culture system is highly desirable. Read More

View Article and Full-Text PDF
November 2021