26,707 results match your criteria derived fetal


Microvesicles and Exosomes Released by Amnion Epithelial Cells Under Oxidative Stress Cause Inflammatory Changes in Uterine Cells.

Biol Reprod 2021 May 7. Epub 2021 May 7.

Division of Maternal-Fetal Medicine and Perinatal Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA.

Extracellular vesicles (EVs) play a crucial role in feto-maternal communication and provide an important paracrine signaling mechanism in pregnancy. We hypothesize that fetal cells-derived exosomes and microvesicles (MVs) under oxidative stress carry unique cargo and traffic through feto-maternal interface, which cause inflammation in uterine cells associated with parturition. Exosomes and MVs, from primary amnion epithelial cell (AEC) culture media under normal or oxidative stress (OS)-induced conditions, were isolated by optimized differential centrifugation method followed by characterization for size (nanoparticle tracking analyzer), shape (transmission electron microscopy), and protein markers (western blot and immunofluorescence). Read More

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Antenatal low-intensity pulsed ultrasound reduces neurobehavioral deficits and brain injury following dexamethasone-induced intrauterine growth restriction.

Brain Pathol 2021 May 7:e12968. Epub 2021 May 7.

Department of Physical Medicine and Rehabilitation, Cheng Hsin General Hospital, Taipei, Taiwan.

Intrauterine growth restriction (IUGR) is a leading cause of perinatal mortality and morbidity, and IUGR survivors are at increased risk of neurodevelopmental deficits. No effective interventions are currently available to improve the structure and function of the IUGR brain before birth. This study investigated the protective effects of low-intensity pulsed ultrasound (LIPUS) on postnatal neurodevelopmental outcomes and brain injury using a rat model of IUGR induced by maternal exposure to dexamethasone (DEX). Read More

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Defined MSC exosome with high yield and purity to improve regenerative activity.

J Tissue Eng 2021 Jan-Dec;12:20417314211008626. Epub 2021 Apr 20.

Department of Biomedical Science, CHA University, Seongnam, Gyeonggi, Republic of Korea.

Exosomes derived from mesenchymal stem cells (MSCs) have been studied as vital components of regenerative medicine. Typically, various isolation methods of exosomes from cell culture medium have been developed to increase the isolation yield of exosomes. Moreover, the exosome-depletion process of serum has been considered to result in clinically active and highly purified exosomes from the cell culture medium. Read More

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Interleukin-6 supplementation improves post-transfer embryonic and fetal development of in vitro-produced bovine embryos.

Theriogenology 2021 Apr 28;170:15-22. Epub 2021 Apr 28.

Department of Animal & Poultry Sciences, Virginia Tech, Blacksburg, VA, USA. Electronic address:

The use of in vitro produced embryos in dairy and beef cattle has increased in recent years, but compromised post-transfer pregnancy success prevents producers from capturing all the benefits this technology can provide. This study explored whether supplementing interleukin-6 (IL6) during in vitro embryo development influences post-transfer development of the embryo-proper, fetus and placenta during early gestation in cattle. Slaughterhouse-derived cumulus oocyte complexes underwent IVM (day -1) and IVF (day 0). Read More

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Maternal sevoflurane exposure induces temporary defects in interkinetic nuclear migration of radial glial progenitors in the fetal cerebral cortex through the Notch signalling pathway.

Cell Prolif 2021 May 6:e13042. Epub 2021 May 6.

Department of Anesthesia, Zhongshan Hospital, Fudan University, Shanghai, China.

Objectives: The effects of general anaesthetics on fetal brain development remain elusive. Radial glial progenitors (RGPs) generate the majority of neurons in developing brains. Here, we evaluated the acute alterations in RGPs after maternal sevoflurane exposure. Read More

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Maturing heart muscle cells: Mechanisms and transcriptomic insights.

Semin Cell Dev Biol 2021 May 2. Epub 2021 May 2.

Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Institute of Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address:

Cardiomyocyte (CM) maturation is the transformation of differentiated fetal CMs into adult CMs that involves changes in morphology, cell function and metabolism, and the transcriptome. This process is, however, incomplete and ultimately arrested in pluripotent stem cell-derived CMs (PSC-CMs) in culture, which hinders their broad biomedical application. For this reason, enormous efforts are currently being made with the goal of generating mature PSC-CMs. Read More

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A Tool for Accurate Stoichiometric Composition of Cryopreservative Media for Fetal and Induced Pluripotent Stem Cell-Derived Human Neural Stem Cells.

Curr Protoc 2021 May;1(5):e123

Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, Sanford Consortium for Regenerative Medicine, La Jolla, California.

Fetal human neural stem cells (fhNSC) are of considerable interest as potential regenerative therapies for neuronal or glial degeneration or destruction resulting from genetic abnormalities, disease, or injury. Realization of this potential requires securing a supply of cells sufficient to meet the needs of transplantation, which are often tens to hundreds of millions of cells per dose. This challenge necessitates the establishment of safe and efficient cell banking protocols. Read More

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Childhood onset nexilin dilated cardiomyopathy: A heterozygous and a homozygous case.

Am J Med Genet A 2021 May 5. Epub 2021 May 5.

Department of Clinical Genetics, Amsterdam University Medical Centers, Amsterdam, Netherlands.

Pathogenic heterozygous NEXN variants are associated with progressive dilated cardiomyopathy (DCM) usually presenting around 50 years of age. We describe an asymptomatic boy who had transient DCM at 3 months of age, that resolved by 4 months. Presently, at 11 years of age, he has normal cardiac function with signs of mild DCM on cardiac MRI. Read More

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MicroRNA-34a activation in tuberous sclerosis complex during early brain development may lead to impaired corticogenesis.

Neuropathol Appl Neurobiol 2021 May 3. Epub 2021 May 3.

Department of (Neuro) Pathology, UMC, University of Amsterdam, Amsterdam Neuroscience, Amsterdam, the Netherlands.

Aims: Tuberous sclerosis complex (TSC) is a genetic disorder associated with dysregulation of the mechanistic target of rapamycin complex 1 (mTORC1) signalling pathway. Neurodevelopmental disorders, frequently present in TSC, are linked to cortical tubers in the brain. We previously reported microRNA-34a (miR-34a) among the most up-regulated miRs in tubers. Read More

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A novel strategy for isolation of mice bone marrow endothelial cells (BMECs).

Stem Cell Res Ther 2021 May 3;12(1):267. Epub 2021 May 3.

Blood Diseases Institute, Xuzhou Medical University, Xuzhou, 221002, China.

Background: In the bone marrow microenvironment (BM), endothelial cells are individual cells that form part of the sinusoidal blood vessels called the "bone marrow endothelial-vascular niche." They account for less than 2% of the bone marrow cells. They play essential functions by generating growth and inhibitory factors that promote the hematopoietic stem cells (HSCs) regulation. Read More

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SERUM GLP-2 is Increased in Association with Excess Gestational Weight Gain.

Am J Perinatol 2021 May 3. Epub 2021 May 3.

Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas.

Objective:  Obesity in pregnancy bears unique maternal and fetal risks. Obesity has also been associated with chronic inflammation, including elevated serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Higher serum lipopolysaccharide (LPS) levels have been implicated in driving this inflammation, a phenomenon called metabolic endotoxemia (ME). Read More

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Molecular analysis of the erythroid phenotype of a patient with BCL11A haploinsufficiency.

Blood Adv 2021 May;5(9):2339-2349

Academic Center for Hemoglobinopathies and Rare Anemias.

The BCL11A gene encodes a transcriptional repressor with essential functions in multiple tissues during human development. Haploinsufficiency for BCL11A causes Dias-Logan syndrome (OMIM 617101), an intellectual developmental disorder with hereditary persistence of fetal hemoglobin (HPFH). Due to the severe phenotype, disease-causing variants in BCL11A occur de novo. Read More

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Foetal cardiac function in third trimester pregnancies with reduced fetal movements.

J Obstet Gynaecol 2021 May 2:1-7. Epub 2021 May 2.

Department of Obstetrics and Gynecology, Uskudar Yunus Emre Hospital, Istanbul, Turkey.

The objective of our study was to investigate the possible relationship between poor perinatal outcome and foetal cardiac functions in pregnant women with reduced foetal movements (RFM). This cross-sectional study included 126 pregnant women with normal foetal movements (Group 1, Controls) and 42 pregnant women over 32 weeks gestation with RFM (Group 2). Group 2 was further divided into two subgroups according to their perinatal outcome: normal perinatal outcome (Group 2a) and poor perinatal outcome (Group 2b). Read More

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Insights Into Extracellular Vesicle/Exosome and miRNA Mediated Bi-Directional Communication During Porcine Pregnancy.

Front Vet Sci 2021 15;8:654064. Epub 2021 Apr 15.

Department Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.

Spontaneous fetal loss is one of the most important challenges that commercial pig industry is still facing in North America. Research over the decade provided significant insights into some of the associated mechanisms including uterine capacity, placental efficiency, deficits in vasculature, and immune-inflammatory alterations at the maternal-fetal interface. Pigs have unique epitheliochorial placentation where maternal and fetal layers lay in opposition without any invasion. Read More

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Cortical spectral matching and shape and volume analysis of the fetal brain pre- and post-fetal surgery for spina bifida: a retrospective study.

Neuroradiology 2021 May 1. Epub 2021 May 1.

School of Biomedical Engineering & Imaging Sciences (BMEIS), King's College London, London, UK.

Purpose: A retrospective study was performed to study the effect of fetal surgery on brain development measured by MRI in fetuses with myelomeningocele (MMC).

Methods: MRI scans of 12 MMC fetuses before and after surgery were compared to 24 age-matched controls without central nervous system abnormalities. An automated super-resolution reconstruction technique generated isotropic brain volumes to mitigate 2D MRI fetal motion artefact. Read More

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Plasma lipidome profiling of newborns with antenatal exposure to Zika virus.

PLoS Negl Trop Dis 2021 Apr 30;15(4):e0009388. Epub 2021 Apr 30.

Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.

The 2015-2016 Zika virus (ZIKV) outbreak in Brazil was remarkably linked to the incidence of microcephaly and other deleterious clinical manifestations, including eye abnormalities, in newborns. It is known that ZIKV targets the placenta, triggering an inflammatory profile that may cause placental insufficiency. Transplacental lipid transport is delicately regulated during pregnancy and deficiency on the delivery of lipids such as arachidonic and docosahexaenoic acids may lead to deficits in both brain and retina during fetal development. Read More

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Evaluation of bone regeneration in mandible large defect using undifferentiated adipose stem cells loaded on gelatin carrier: An animal model case study.

J Dent Res Dent Clin Dent Prospects 2021 13;15(1):22-29. Epub 2021 Feb 13.

Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Large mandibular defects are considered difficult reconstructive challenges for oral and maxillofacial surgeons. Cell therapy, as an alternative technique, might increase the speed of bone regeneration. This study aimed to investigate bone regeneration in large defects of dog mandibles using allogenic adipose-derived stem cells on gelatin foam as a cell carrier. Read More

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February 2021

Associations of circulating folate, vitamin B12 and homocysteine concentrations in early pregnancy and cord blood with epigenetic gestational age: the Generation R Study.

Clin Epigenetics 2021 Apr 29;13(1):95. Epub 2021 Apr 29.

The Generation R Study Group (Na-2918), Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.

Background: Circulating folate, vitamin B12 and homocysteine concentrations during fetal development have been associated with health outcomes in childhood. Changes in fetal DNA methylation may be an underlying mechanism. This may be reflected in altered epigenetic aging of the fetus, as compared to chronological aging. Read More

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Annexins A2, A6 and Fetuin-A Affect the Process of Mineralization in Vesicles Derived from Human Osteoblastic hFOB 1.19 and Osteosarcoma Saos-2 Cells.

Int J Mol Sci 2021 Apr 13;22(8). Epub 2021 Apr 13.

Laboratory of Biochemistry of Lipids, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Str., 02-093 Warsaw, Poland.

The mineralization process is initiated by osteoblasts and chondrocytes during intramembranous and endochondral ossifications, respectively. Both types of cells release matrix vesicles (MVs), which accumulate P and Ca and form apatites in their lumen. Tissue non-specific alkaline phosphatase (TNAP), a mineralization marker, is highly enriched in MVs, in which it removes inorganic pyrophosphate (PP), an inhibitor of apatite formation. Read More

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Pattern of Tumor-Infiltrating Lymphocytes in Mixed Epithelial and Stromal Tumor of the Kidney: A Review of Five Cases.

Cells 2021 Apr 16;10(4). Epub 2021 Apr 16.

Department of Urology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea.

Mixed epithelial and stromal tumor of the kidney (MESTK), a benign rare tumor with malignant transformation potential, is thought to be derived from fetal or immature cells originating from the mesonephric and Müllerian ducts. However, due to its rarity, little is known about the anti-tumor immune responses in MESTK. Herein, we present five cases of MESTK and evaluate the population of tumor-infiltrating lymphocytes (TILs) using a freshly obtained MESTK sample. Read More

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Crassolide Suppresses Dendritic Cell Maturation and Attenuates Experimental Antiphospholipid Syndrome.

Molecules 2021 Apr 24;26(9). Epub 2021 Apr 24.

Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan.

Antiphospholipid syndrome (APS) is an autoimmune disease characterized by the production of β2-glycoprotein I (β2GPI)-dependent autoantibodies, with vascular thrombosis or obstetrical complications. Around 20% of APS patients are refractory to current treatments. Crassolide, a cembranoid diterpene extracted from soft corals, is a potential therapeutic candidate. Read More

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Use of the Renal Artery Doppler to Identify Small for Gestational Age Fetuses at Risk for Adverse Neonatal Outcomes.

J Clin Med 2021 Apr 23;10(9). Epub 2021 Apr 23.

Department of Women and Child Heath, University of Padua School of Medicine, 35122 Padova, Italy.

Objective: To measure the sensitivity and positive predictive value (PPV) for an adverse neonatal outcome among growth-restricted fetuses (FGR) comparing the cerebral-placental ratio (CPR) with the cerebral-renal ratio (CRR).

Methods: Retrospective analysis of 92 women who underwent prenatal ultrasound at the University of Maryland and the University of Padua. Renal, middle cerebral and umbilical artery Doppler waveforms were recorded for all scans during the third trimester. Read More

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Decreased Lymphangiogenic Activities and Genes Expression of Cord Blood Lymphatic Endothelial Progenitor Cells (VEGFR3/Pod/CD11b Cells) in Patient with Preeclampsia.

Int J Mol Sci 2021 Apr 19;22(8). Epub 2021 Apr 19.

Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University Health System, Seoul 03722, Korea.

The abnormal development or disruption of the lymphatic vasculature has been implicated in metabolic and hypertensive diseases. Recent evidence suggests that the offspring exposed to preeclampsia (PE) in utero are at higher risk of long-term health problems, such as cardiovascular and metabolic diseases in adulthood, owing to in utero fetal programming. We aimed to investigate lymphangiogenic activities in the lymphatic endothelial progenitor cells (LEPCs) of the offspring of PE. Read More

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Rejuvenated Stem/Progenitor Cells for Cartilage Repair Using the Pluripotent Stem Cell Technology.

Bioengineering (Basel) 2021 Apr 10;8(4). Epub 2021 Apr 10.

Steadman Philippon Research Institute, Vail, CO 81657, USA.

It is widely accepted that chondral defects in articular cartilage of adult joints are never repaired spontaneously, which is considered to be one of the major causes of age-related degenerative joint disorders, such as osteoarthritis. Since mobilization of subchondral bone (marrow) cells and addition of chondrocytes or mesenchymal stromal cells into full-thickness defects show some degrees of repair, the lack of self-repair activity in adult articular cartilage can be attributed to lack of reparative cells in adult joints. In contrast, during a fetal or embryonic stage, joint articular cartilage has a scar-less repair activity, suggesting that embryonic joints may contain cells responsible for such activity, which can be chondrocytes, chondroprogenitors, or other cell types such as skeletal stem cells. Read More

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Synthesis, Complexation Properties, and Evaluation of New Aminodiphosphonic Acids as Vector Molecules for Ga Radiopharmaceuticals.

Molecules 2021 Apr 18;26(8). Epub 2021 Apr 18.

Laborotary of Novel Physicochemical Problems, Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Leninskii pr. 31/4, 119071 Moscow, Russia.

Two new aminodiphosphonic acids derived from salicylic acid and its phosphonic analogue were prepared through a simple and efficient synthesis. 2-[(2-Amino-2,2-diphosphono)ethyloxy]-benzoic acid and 2-[(2-amino-2,2-diphosphono)ethyloxy]-5-ethyl-phenylphosphonic acid were evaluated for their applicability as Ga binding bone-seeking agents. Protonation constants of and and stability constants of the Ga complexes with and in water were determined. Read More

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Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage Polarization.

Int J Mol Sci 2021 Apr 21;22(9). Epub 2021 Apr 21.

Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, 72076 Tübingen, Germany.

Mesenchymal stem cells from bone marrow have powerful immunomodulatory capabilities. The interactions between jaw periosteal cells (JPCs) and macrophages are not only relevant for the application of JPCs in regenerative medicine, but this understanding could also help treating diseases like osteonecrosis of the jaw. In previous studies, we analyzed, for the first time, immunomodulatory features of 2D- and 3D-cultured JPCs. Read More

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Pregnancy-Related Extracellular Vesicles Revisited.

Int J Mol Sci 2021 Apr 9;22(8). Epub 2021 Apr 9.

Department of Surgical Oncology, Prof. Dr. Alexandru Trestioreanu Oncology Institute, Carol Davila University of Medicine and Pharmacy, 252 Fundeni Rd., 022328 Bucharest, Romania.

Extracellular vesicles (EVs) are small vesicles ranging from 20-200 nm to 10 μm in diameter that are discharged and taken in by many different types of cells. Depending on the nature and quantity of their content-which generally includes proteins, lipids as well as microRNAs (miRNAs), messenger-RNA (mRNA), and DNA-these particles can bring about functional modifications in the receiving cells. During pregnancy, placenta and/or fetal-derived EVs have recently been isolated, eliciting interest in discovering their clinical significance. Read More

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The Impact of a Plant-Based Diet on Gestational Diabetes: A Review.

Antioxidants (Basel) 2021 Apr 2;10(4). Epub 2021 Apr 2.

Independent Researcher, Via Venezuela 66, 98121 Messina, Italy.

Gestational diabetes mellitus (GDM) represents a challenging pregnancy complication in which women present a state of glucose intolerance. GDM has been associated with various obstetric complications, such as polyhydramnios, preterm delivery, and increased cesarean delivery rate. Moreover, the fetus could suffer from congenital malformation, macrosomia, neonatal respiratory distress syndrome, and intrauterine death. Read More

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Comprehensive Profiling of Secretome Formulations from Fetal- and Perinatal Human Amniotic Fluid Stem Cells.

Int J Mol Sci 2021 Apr 2;22(7). Epub 2021 Apr 2.

Experimental Biology Unit, Department of Experimental Medicine (DIMES), University of Genova, 16132 Genova, Italy.

We previously reported that c-KIT+ human amniotic-fluid derived stem cells obtained from leftover samples of routine II trimester prenatal diagnosis (fetal hAFS) are endowed with regenerative paracrine potential driving pro-survival, anti-fibrotic and proliferative effects. hAFS may also be isolated from III trimester clinical waste samples during scheduled C-sections (perinatal hAFS), thus offering a more easily accessible alternative when compared to fetal hAFS. Nonetheless, little is known about the paracrine profile of perinatal hAFS. Read More

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The Expression of Anti-Müllerian Hormone Type II Receptor (AMHRII) in Non-Gynecological Solid Tumors Offers Potential for Broad Therapeutic Intervention in Cancer.

Biology (Basel) 2021 Apr 7;10(4). Epub 2021 Apr 7.

GamaMabs Pharma, Centre Pierre Potier, F-31100 Toulouse, France.

The anti-Müllerian hormone (AMH) belongs to the TGF-β family and plays a key role during fetal sexual development. Various reports have described the expression of AMH type II receptor (AMHRII) in human gynecological cancers including ovarian tumors. According to qRT-PCR results confirmed by specific In-Situ Hybridization (ISH) experiments, AMHRII mRNA is expressed in an extremely restricted number of normal tissues. Read More

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