2,535 results match your criteria depletion albumin

Development, Validation, and Comparison of Two Mass Spectrometry Methods (LC-MS/HRMS and LC-MS/MS) for the Quantification of Rituximab in Human Plasma.

Molecules 2021 Mar 4;26(5). Epub 2021 Mar 4.

Biochemistry and Pharmacology-Toxicology Laboratory, Lyon-Sud Hospital, Hospices Civils de Lyon, F-69495 Pierre Bénite, France.

Rituximab is a chimeric immunoglobulin G1-kappa (IgG1κ) antibody targeting the CD20 antigen on B-lymphocytes. Its applications are various, such as for the treatment of chronic lymphoid leukemia or non-Hodgkin's lymphoma in oncology, and it can also be used in the treatment of certain autoimmune diseases. Several studies support the interest in therapeutic drug monitoring to optimize dosing regimens of rituximab. Read More

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A Simple Method for In-Depth Proteome Analysis of Mammalian Cell Culture Conditioned Media Containing Fetal Bovine Serum.

Int J Mol Sci 2021 Mar 4;22(5). Epub 2021 Mar 4.

Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.

A conditioned medium of a cell culture is widely used for various biological applications and frequently analyzed to characterize the functional proteins responsible for observed biological functions. However, a large number of abundant proteins in fetal bovine serum (FBS), usually included in the conditioned medium of a mammalian cell culture medium, hampers in-depth proteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). For a deep proteomic analysis of a conditioned medium by LC-MS/MS, we developed a simple albumin depletion approach coupled with data-independent acquisition (DIA)-mode LC-MS/MS for the conditioned medium of mammalian cells in this study. Read More

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Allysine and α-Aminoadipic Acid as Markers of the Glyco-Oxidative Damage to Human Serum Albumin under Pathological Glucose Concentrations.

Antioxidants (Basel) 2021 Mar 17;10(3). Epub 2021 Mar 17.

Meat and Meat Products Research Institute (IPROCAR), Food Technology, University of Extremadura, 10003 Cáceres, Spain.

Understanding the molecular basis of the disease is of the utmost scientific interest as it contributes to the development of targeted strategies of prevention, diagnosis, and therapy. Protein carbonylation is a typical feature of glyco-oxidative stress and takes place in health disorders such as diabetes. Allysine as well as its oxidation product, the α-amino adipic acid (α-AA) have been found to be markers of diabetes risk whereas little is known about the chemistry involved in its formation under hyperglycemic conditions. Read More

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Is there a renoprotective value to leukodepletion during heart valve surgery? A randomized controlled trial (ROLO).

J Cardiothorac Surg 2021 Mar 26;16(1):58. Epub 2021 Mar 26.

Clinical Trials and Evaluation Unit, Bristol Medical School, University of Bristol, Bristol, BS2 8HW, UK.

Background: Acute Kidney Injury (AKI) adversely affects outcomes after cardiac surgery. A major mediator of AKI is the activation of leukocytes through exposure to the cardiopulmonary bypass circuit. We evaluate the use of leukodepletion filters throughout bypass to protect against post-operative AKI by removing activated leukocytes during cardiac surgery. Read More

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AMPK-dependent phosphorylation is required for transcriptional activation of TFEB and TFE3.

Autophagy 2021 Mar 18:1-19. Epub 2021 Mar 18.

Goodman Cancer Research Center, McGill University, Montréal, Québec, Canada.

Increased macroautophagy/autophagy and lysosomal activity promote tumor growth, survival and chemo-resistance. During acute starvation, autophagy is rapidly engaged by AMPK (AMP-activated protein kinase) activation and MTOR (mechanistic target of rapamycin kinase) complex 1 (MTORC1) inhibition to maintain energy homeostasis and cell survival. TFEB (transcription factor E3) and TFE3 (transcription factor binding to IGHM enhancer 3) are master transcriptional regulators of autophagy and lysosomal activity and their cytoplasm/nuclear shuttling is controlled by MTORC1-dependent multisite phosphorylation. Read More

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Human serum albumin-imprinted polymers with high capacity and selectivity for abundant protein depletion.

Acta Biomater 2021 Mar 17. Epub 2021 Mar 17.

Key Laboratory of Functional Polymer Materials and State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China; School of Material Science and Engineering, Tiangong University, Tianjin 300387, China. Electronic address:

Depletion of human serum albumin (HSA), the most abundant protein in human plasma, from serum/plasma is a prerequisite before their proteomic analysis. Molecularly imprinted polymers (MIPs) using HSA as a template have been designed for this purpose, but suffer from a low sorption capacity and low selectivity. Here, a new HSA-imprinted polymer was synthesized using N-isopropylacrylamide (NIPAM) as the main monomer; acrylamide (AAm), methacrylic acid (MAA), and dimethylaminoethyl methacrylate (DMAEMA) as functional monomers; and oligoglutamic acid-based peptide crosslinker (PC) as a crosslinker at pH 5. Read More

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Sarcopenia is a strong predictive factor of clinical and oncological outcomes following curative colorectal cancer resection.

ANZ J Surg 2021 Mar 7. Epub 2021 Mar 7.

Colorectal Surgical Unit, St Vincent's Hospital, Melbourne, Victoria, Australia.

Background: Skeletal muscle depletion and subsequent functional loss is common in gastrointestinal malignancy. Usual markers of nutritional status may not be part of routine workup. The predictive value of sarcopenia was assessed and compared with clinically utilized factors. Read More

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Protective role of Clitoria ternatea L. flower extract on methylglyoxal-induced protein glycation and oxidative damage to DNA.

BMC Complement Med Ther 2021 Mar 1;21(1):80. Epub 2021 Mar 1.

Department of Food Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, 10400, Thailand.

Background: Methylglyoxal (MG) is a highly reactive dicarbonyl precursor for the formation of advanced glycation end products (AGEs) associated with age-related diseases, including diabetes and its complications. Clitoria ternatea L. flower has been reported to possess antioxidant and antiglycating properties. Read More

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Symmetrical peripheral gangrene in critical illness.

Transfus Apher Sci 2021 Apr 13;60(2):103094. Epub 2021 Feb 13.

Department of Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; Department of Pathology and Molecular Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; McMaster Centre for Transfusion Research, Canada; Hamilton Regional Laboratory Medicine Program, Hamilton, Ontario, Canada; St. Joseph's Healthcare, Hamilton, Ontario, Canada.

Symmetrical peripheral gangrene (SPG) is a disabling complication that affects a small proportion of patients who survive critical illness. Its pathogenesis reflects profoundly disturbed procoagulant-anticoagulant balance in susceptible tissue beds secondary to circulatory shock (cardiogenic, septic). There is a characteristic SPG triad: (a) shock (hypotension, lactic acidemia, normoblastemia, multiple organ dysfunction), (b) disseminated intravascular coagulation (DIC), and (c) natural anticoagulant depletion (protein C, antithrombin). Read More

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Formulating Diet for Transitional Feeding in Tuberculous Meningitis Patient during Hospital Care and through to Period of Home Healthcare.

J Nutr Sci Vitaminol (Tokyo) 2020 ;66(Supplement):S41-S46

Departement of Nutrition, Faculty of Medicine, Hasanuddin University.

Tuberculosis (TB) affects one-third of the world's population and is the leading cause of human mortality and morbidity. Tuberculous meningitis (TBM) is the most severe type of extrapulmonary diasease. Medical treatment and nutritional management is the most efficient and cost-effective of all health interventions. Read More

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January 2020

Nutrition Therapy in Critically Ill Overweight Elderly Patient with Heart Failure, Myocardial Infarction, Pneumonia, and Chronic Kidney Disease.

J Nutr Sci Vitaminol (Tokyo) 2020 ;66(Supplement):S25-S31

Department of Nutritional Science, Faculty of Medicine, Hasanuddin University.

Overweight or obesity will increase the risk of morbidity and mortality from cardiovascular disease. In older people, the risk is higher, but also paradoxically associated with lower mortality rates. Overweight patients vary in body composition and when it coupled with limited reliable sources to make caloric requirements estimation will make nutrition therapy extremely challenging. Read More

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January 2020

Therapeutic targeting of DGKA-mediated macropinocytosis leads to phospholipid reprogramming in Tuberous Sclerosis Complex.

Cancer Res 2021 Feb 16. Epub 2021 Feb 16.

Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, Harvard Medical School

Lymphangioleiomyomatosis (LAM) is a rare destructive lung disease affecting primarily women and is the primary lung manifestation of tuberous sclerosis complex (TSC). In LAM, biallelic loss of TSC1/2 leads to hyperactivation of mTORC1 and inhibition of autophagy. To determine how the metabolic vulnerabilities of TSC2-deficient cells can be targeted, we performed a high throughput screen utilizing the "Repurposing" library at the Broad Institute, with or without the autophagy inhibitor chloroquine. Read More

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February 2021

Biodegradable Silica-Based Nanotheranostics for Precise MRI/NIR-II Fluorescence Imaging and Self-Reinforcing Antitumor Therapy.

Small 2021 Mar 11;17(10):e2006508. Epub 2021 Feb 11.

Department of Radiology, third hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan, 030032, China.

Multi-modality cancer diagnosis techniques based on the second near-infrared window fluorescence (NIR-II FL, 1000-1700 nm) imaging have become the focus of research attention. For such multimodality probes, how to take advantage of the tumor microenvironments (TME) characteristics to better image diseases and combine efficient therapeutics to achieve theranostics is still a big challenge. Herein, a novel TME-activated nanosystem (FMSN-MnO -BCQ) employing degradable silica-based nanoplatform is designed, adjusting the ratio of intratumoral hydrogen peroxide (H O )/glutathione (GSH) for magnetic resonance imaging (MRI)/NIR-II FL imaging and self-reinforcing chemodynamic therapy (CDT). Read More

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Effect of liver total sphingomyelin synthase deficiency on plasma lipid metabolism.

Biochim Biophys Acta Mol Cell Biol Lipids 2021 May 2;1866(5):158898. Epub 2021 Feb 2.

Department of Cell Biology, SUNY Downstate Medical Center, United States of America; Molecular and Cellular Cardiology Program, VA New York Harbor Healthcare System, Brooklyn, United States of America. Electronic address:

Sphingomyelin (SM) is one major phospholipids on lipoproteins. It is enriched on apolipoprotein B-containing particles, including very low-density lipoprotein (VLDL) and its catabolites, low-density lipoprotein (LDL). SM is synthesized by sphingomyelin synthase 1 and 2 (SMS1 and SMS2) which utilizes ceramide and phosphatidylcholine, as two substrates, to produce SM and diacylglyceride. Read More

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Alterations in hematological and biochemical parameters, oxidative stress, and immune response in Takifugu rubripes under acute ammonia exposure.

Comp Biochem Physiol C Toxicol Pharmacol 2021 May 22;243:108978. Epub 2021 Jan 22.

Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, People's Republic of China. Electronic address:

Ammonia is a major pollutant in aquatic environments and poses a considerable threat to the survival of fish. In this study, we investigated the toxic effects of ammonia on the hematological and biochemical parameters, oxidative stress, and immune responses in Takifugu rubripes. Juvenile T. Read More

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Fetal bovine serum albumin inhibits antimicrobial peptide activity and binds drug only in complex with α1-antitrypsin.

Sci Rep 2021 Jan 14;11(1):1267. Epub 2021 Jan 14.

Institute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan.

Several antimicrobial peptides (AMPs) have been developed for the treatment of infections caused by antibiotic-resistant microbes, but their applications are primarily limited to topical infections because in circulation they are bound and inhibited by serum proteins. Here we have found that some AMPs, such as TP4 from fish tilapia, and drugs, such as antipyretic ibuprofen, were bound by bovine serum albumin only in complex with α1-antitrypsin which is linked by disulfide bond. They existed in dimeric complex (2 albumin -2 α1-antitrypsin) in the bovine serum only at fetal stage, but not after birth. Read More

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January 2021

Hypochlorite-induced aggregation of fibrinogen underlies a novel antioxidant role in blood plasma.

Redox Biol 2021 Apr 30;40:101847. Epub 2020 Dec 30.

Flinders Health and Medical Research Institute and College of Medicine and Public Health, Flinders University, Bedford Park, Australia. Electronic address:

Fibrinogen, a major constituent of blood plasma, is highly susceptible to reaction with biological oxidants. It has been proposed that fibrinogen plays a role in antioxidant defence, but oxidation of fibrinogen is also known to disrupt normal blood clotting and is implicated in the pathology of atherosclerosis. In the present study, we show that the biological oxidant hypochlorite promotes the formation of soluble high molecular weight fibrinogen assemblies ≥40 × 10 Da, that do not accumulate when fibrinogen is induced to aggregate by other stresses such as heating or hydroxyl-mediated damage in vitro. Read More

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Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?

ACS Appl Bio Mater 2020 Dec 8;3(12):8344-8351. Epub 2020 Dec 8.

Nanotechnology and Functional Materials, Department of Materials Science and Engineering, Uppsala University, Box 35, 751 03 Uppsala, Sweden.

Virus safety of fetal bovine serum (FBS) is a critical issue for cell culture and clinical applications of cell therapies. The size exclusion filtration of FBS-supplemented cell culture media through small-size virus retentive filter paper is presented to investigate its effect on cell culture. A substantial proportion of proteins (ca. Read More

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December 2020

[Ultradian rhythms and oxidative stress in the tissue of the lymph nodes in ontogenesis.]

Adv Gerontol 2020 ;33(4):771-777

Astrakhan State University, 20a Tatishcheva str., Astrakhan 414000, Russian Federation.

We studied the age-related features of the ultradian rhythms of levels of total protein and albumin in the lymph nodes under normal condition and chronic toxic oxidative stress condition using the chronobiological approach and the biochemical methods. During the experiment, we compared the rhythmic activity of levels of total protein and albumin in the rat lymph nodes in ontogenesis and during chronic exposure to sulfur-containing natural gas. The revealed fluctuations of the levels of total protein and albumin can be characterized as around-hourly (ultradian) rhythms, which period is approximately 20-40 to 60 minutes. Read More

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December 2020

PepS: An Innovative Microfluidic Device for Bedside Whole Blood Processing before Plasma Proteomics Analyses.

Anal Chem 2021 01 15;93(2):683-690. Epub 2020 Dec 15.

Univ. Grenoble Alpes, CEA, Inserm, IRIG, BGE, EDyP, F-38000 Grenoble, FRANCE.

Immunoassays have been used for decades in clinical laboratories to quantify proteins in serum and plasma samples. However, their limitations make them inappropriate in some cases. Recently, mass spectrometry (MS) based proteomics analysis has emerged as a promising alternative method when seeking to assess panels of protein biomarkers with a view to providing protein profiles to monitor health status. Read More

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January 2021

The preliminary comparative results between Covid-19 and non-Covid-19 patients in Western China.

BMC Infect Dis 2020 Dec 9;20(1):935. Epub 2020 Dec 9.

Department of Administrative Office, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: This study aims to investigate the comparative clinical characteristics of Covid-19 and non-Covid-19 patients.

Methods: Fifteen Covid-19 and 93 non-Covid-19 patients were included in RNA testing. All epidemiological and clinical data were collected and analyzed, and then comparative results were carried out. Read More

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December 2020

Mesangial cells regulate the single nephron GFR and preserve the integrity of the glomerular filtration barrier: An intravital multiphoton microscopy study.

Acta Physiol (Oxf) 2021 Apr 11;231(4):e13592. Epub 2021 Mar 11.

Institute of Physiology, University of Regensburg, Regensburg, Germany.

Aim: The intraglomerular mesangial cells are located between the glomerular capillaries. Here we hypothesized that mesangial cells regulate the single nephron glomerular filtration rate (snGFR) and that mesangial cells support the integrity of the glomerular filtration barrier.

Methods: We assessed the function of mesangial cells in vivo by multiphoton microscopy. Read More

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Circ_0109046 Promotes the Progression of Endometrial Cancer via Regulating miR-136/HMGA2 Axis.

Cancer Manag Res 2020 2;12:10993-11003. Epub 2020 Nov 2.

Department of Gynaecology, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, People's Republic of China.

Background: Endometrial cancer (EC) is one of the most common gynecological malignancies. Circular RNAs (circRNAs) play crucial roles in the occurrence and development of tumors. This research aimed to explore the function and potential mechanism of human serum albumin (hsa)_circ_0109046 in EC. Read More

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November 2020

Systematic study of the selenium fractionation in human plasma from a cancer prevention trial using HPLC hyphenated to ICP-MS and ESI-MS/MS.

Anal Bioanal Chem 2021 Jan 2;413(2):331-344. Epub 2020 Nov 2.

LGC Limited, Queens Road, Teddington, Middlesex, TW11 0LY, UK.

This work represents the first systematic speciation study of selenium (Se) in plasma from subjects participating in a pilot study for a cancer prevention trial (PRECISE). This involved supplementation of elderly British and Danish individuals with selenised yeast for 6 months and 5 years, respectively, at 100, 200, and 300 μg Se/day or placebo. Speciation data was obtained for male plasma using HPLC-ICP-MS and HPLC-ESI-MS/MS. Read More

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January 2021

SGLT2 Inhibition for CKD and Cardiovascular Disease in Type 2 Diabetes: Report of a Scientific Workshop Sponsored by the National Kidney Foundation.

Am J Kidney Dis 2021 01 26;77(1):94-109. Epub 2020 Oct 26.

George Institute for Global Health, UNSW Sydney, Australia.

Diabetes is the most frequent cause of chronic kidney disease (CKD), leading to nearly half of all cases of kidney failure requiring replacement therapy. The principal cause of death among patients with diabetes and CKD is cardiovascular disease (CVD). Sodium/glucose cotransporter 2 (SGLT2) inhibitors were developed to lower blood glucose levels by inhibiting glucose reabsorption in the proximal tubule. Read More

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January 2021

Pre-sarcopenia determines post-progression outcomes in advanced hepatocellular carcinoma after sorafenib failure.

Sci Rep 2020 10 27;10(1):18375. Epub 2020 Oct 27.

Division of Gastroenterology and Hepatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.

Many second-line therapies are recently approved for patients with advanced hepatocellular carcinoma (HCC), in whom protein malnutrition is prevalent that would affect treatment outcomes. In this study, we aimed to investigate the role of pre-sarcopenia and muscle restoration in patients with sorafenib-failed advanced HCC. From August 2012 to March 2017, 385 patients who developed radiology-proven HCC progression after sorafenib treatment were enrolled in the study. Read More

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October 2020

Isolation of extracellular vesicles with multivalent aptamers.

Analyst 2021 Jan;146(1):253-261

The Pq Laboratory of Micro/Nano BiomeDx, Department of Biomedical Engineering, Binghamton University-SUNY, Binghamton, NY 13902, USA.

Extracellular vesicles (EVs) are lipid-enclosed submicron-sized vesicles that are secreted by all eukaryotic cells. EVs can selectively encapsulate tissue-specific small molecules from parent cells and efficiently deliver them to recipient cells. As signal mediators of intercellular communication, the molecules packaged in EVs play critical roles in the pathophysiology of diseases. Read More

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January 2021

SGLT2 Inhibition for CKD and Cardiovascular Disease in Type 2 Diabetes: Report of a Scientific Workshop Sponsored by the National Kidney Foundation.

Diabetes 2021 Jan 26;70(1):1-16. Epub 2020 Oct 26.

George Institute for Global Health, UNSW Sydney, Australia.

Diabetes is the most frequent cause of chronic kidney disease (CKD), leading to nearly half of all cases of kidney failure requiring replacement therapy. The principal cause of death among patients with diabetes and CKD is cardiovascular disease (CVD). Sodium/glucose cotransporter 2 (SGLT2) inhibitors were developed to lower blood glucose levels by inhibiting glucose reabsorption in the proximal tubule. Read More

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January 2021

S-Methylcysteine (SMC) Ameliorates Intestinal, Hepatic, and Splenic Damage Induced by Infection Via Targeting Inflammatory Modulators and Oxidative Stress in Swiss Albino Mice.

Biomedicines 2020 Oct 15;8(10). Epub 2020 Oct 15.

Department of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr el-Sheikh 33516, Egypt.

Cryptosporidiosis has been proposed to be one of the major causes of diarrhoeal disease in humans worldwide that possesses zoonotic concern. Thereby, this study investigated the potential effects of s-Methylcysteine (SMC) on the parasite in vivo followed by the measurement of cytokines, oxidative stress parameters, and an investigation of the major histopathological changes. Sixty male Swiss albino mice weighing 20-25 g were allocated equally into five groups and orally administered saline only (control), SMC only (SMC50) (50 mg/kg b. Read More

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October 2020

Long noncoding RNA MIAT regulates primary human retinal pericyte pyroptosis by modulating miR-342-3p targeting of CASP1 in diabetic retinopathy.

Exp Eye Res 2021 01 13;202:108300. Epub 2020 Oct 13.

Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. Electronic address:

Diabetic retinopathy (DR) is the leading cause of visual impairment and acquired blindness among adults worldwide. Retinal microvascular pericyte deficiency is one of the earliest pathological changes associated with DR, and long noncoding RNA myocardial infarction-associated transcript (MIAT) has been implicated as a crucial regulator of microvascular dysfunction in DR. Pyroptosis is a caspase-1-dependent proinflammatory form of cell death, and in the present study, we investigated the potential pyroptosis of primary human retinal pericytes (HRPCs) and the mechanism by which MIAT is involved in this process. Read More

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January 2021