DNA Cell Biol 2021 Mar 22. Epub 2021 Mar 22.
Health Science Institute, School of Dentistry, Paulista University-UNIP, São Paulo, Brazil.
Periodontal ligament cells (PDLCs) have well documented osteogenic potential; however, this commitment can be highly heterogenous, limiting their applications in tissue regeneration. In this study, we use PDLC populations characterized by high and low osteogenic potential (h-PDLCs and l-PDLCs, respectively) to identify possible sources of such heterogeneity and to investigate whether the osteogenic differentiation can be enhanced by epigenetic modulation. In h-PDLCs, low basal expression levels of pluripotency markers (, ), DNA methyltransferases (, ), and enzymes involved in active DNA demethylation (, ) were prerequisite to high osteogenic potential. Read More