5,917 results match your criteria dental mineralization

Synthesis and characterization of nano-hydroxyapatite from Sardinella longiceps fish bone and its effects on human osteoblast bone cells.

J Mech Behav Biomed Mater 2021 Apr 14;119:104501. Epub 2021 Apr 14.

CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608 502, India.

Organic debris in the form of fish bone wastes account to several thousand tons annually. In recent years, researchers have turned attention towards the bioconversion of organic debris into materials with biomedical applications. Accordingly, the present study synthesized nano-Hydroxyapatite (n-HAP) from bones of discarded Sardinella longiceps by the alkaline hydrolysis method. Read More

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Classical isoforms of protein kinase C (PKC) and Akt regulate the osteogenic differentiation of human dental follicle cells via both β-catenin and NF-κB.

Stem Cell Res Ther 2021 Apr 14;12(1):242. Epub 2021 Apr 14.

Department of Oral and Maxillofacial Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.

Background: Human dental follicle cells (DFCs) are the precursor cells of the periodontium with a high potential for regenerative therapies of (alveolar) bone. However, the molecular mechanisms of osteogenic differentiation are inadequately understood. Classical isoforms of protein kinase C (PKC) are reported to inhibit osteogenesis of stem/precursor cells. Read More

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[Prevalence of hypophosphatasia in adult patients in rheumatology].

Z Rheumatol 2021 Apr 14. Epub 2021 Apr 14.

Klinik für Innere Medizin III, Onkologie, Hämatologie, Rheumatologie und Klinische Immunologie, Universitätsklinikum Bonn, Venusberg Campus 1, 53127, Bonn, Deutschland.

Background: Hypophosphatasia (HPP) is a genetic disorder caused by one or more mutations in the alkaline phosphatase (ALP) gene, responsible for encoding tissue-specific ALP and for the mineralization process.

Objective: Identification of the prevalence of HPP in rheumatology patients.

Material And Methods: Medical records of all adult rheumatology patients with pathologically low total ALP levels (<35 U/L) treated in the Department of Rheumatology at the Clinic of Internal Medicine III, University Hospital Bonn between January 2017 and June 2019, were retrospectively examined for clinical signs as well as for results of genetic tests for HPP. Read More

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Generation of a new mouse line with conditionally activated signaling through the BMP receptor, ACVR1: A tool to characterize pleiotropic roles of BMP functions.

Genesis 2021 Apr 14:e23419. Epub 2021 Apr 14.

Department of Biologic and Materials Sciences & Prosthodontics, School of Dentistry, University of Michigan, MI, USA.

BMP signaling plays pleiotropic roles in various tissues during embryogenesis and after birth. We have previously generated a constitutively activated Acvr1(ca-Acvr1) transgenic mouse line (line L35) through pronuclei injection to investigate impacts of enhanced BMP signaling in a tissue specific manner. However, line L35 shows a restricted expression pattern of the transgene. Read More

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Rodent incisor and molar dental follicles show distinct characteristics in tooth eruption.

Arch Oral Biol 2021 Apr 2;126:105117. Epub 2021 Apr 2.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China. Electronic address:

Objective: Rodent incisors and molars show different eruption patterns. Dental follicles and their interaction with dental epithelia play key roles in tooth eruption. However, little is known about the differences between incisor dental follicle (IF) and molar dental follicle (MF) during tooth eruption of rodents. Read More

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Dental Pulp Stem Cells on Implant Surface: An In Vitro Study.

Biomed Res Int 2021 23;2021:3582342. Epub 2021 Mar 23.

Department of Biomedical and Dental Sciences and Morphological and Functional Imaging, Messina University, Messina 98100, Italy.

In the field of biology and medicine, one hears often about stem cells and their potential. The dental implant new surfaces, subjected to specific treatments, perform better and allow for quicker healing times and better clinical performance. The purpose of this study is to evaluate from a biological point of view the interaction and cytotoxicity between stem cells derived from dental pulp (DPSCs) and titanium surfaces. Read More

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[Effect of amoxicillin on the enamel mineralization of SD rats].

Zhonghua Kou Qiang Yi Xue Za Zhi 2021 Apr;56(4):355-361

Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases & Department of Preventive Dentistry, College of Stomatology, Xi' an Jiaotong University, Xi'an 710004, China.

To determine whether amoxicillin had an effect on the enamel mineralization of SD rats. Eighteen SD rats were randomly divided into three groups. The rats in the control group were given distilled water. Read More

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Synthesis and characterization of a photo-cross-linked bioactive polycaprolactone-based osteoconductive biocomposite.

J Biomed Mater Res A 2021 Apr 8. Epub 2021 Apr 8.

Department of Dental Biomaterials, School of Dentistry/Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

In this study, a light cross-linkable biocomposite scaffold based on a photo-cross-linkable poly (propylene fumarate) (PPF)-co-polycaprolactone (PCL) tri-block copolymer was synthesized and characterized. The developed biodegradable scaffold was further modified with β-tricalcium phosphate (β-TCP) bioceramic for bone tissue engineering applications. The developed biocomposite was characterized using H nuclear magnetic resonance and Fourier transform infrared spectroscopy. Read More

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JNK Signaling Pathway Mediates Fluoride-Induced Upregulation of CK1α during Enamel Formation.

Caries Res 2021 Apr 7:1-9. Epub 2021 Apr 7.

The First Affiliated Hospital of Henan University, Kaifeng, China.

Fluorosis is a defect in the enamel mineral content caused by excessive fluoride intake during amelogenesis; the interaction of various factors in the development and progression of fluorosis has not been defined. Casein kinase 1α (CK1α) is constitutively active in cells and is involved in diverse cellular processes; however, its expression in fluorosis has not been measured. This study aimed to investigate the effects of fluoride on CK1α expression and to assess the regulation of molecular signaling involving fluoride and CK1α during enamel development. Read More

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GSK3β rephosphorylation rescues ALPL deficiency-induced impairment of odontoblastic differentiation of DPSCs.

Stem Cell Res Ther 2021 Apr 6;12(1):225. Epub 2021 Apr 6.

National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, Precision Medicine Institute, The Second Affiliated Hospital of Xi'an Jiaotong University, No.157 Xiwu Road, Xi'an, 710004, China.

Background: Premature exfoliation of the deciduous teeth is a common manifestation in childhood patients with hypophosphatasia (HPP), which is an autosomal inherited disease caused by ALPL mutations. Dysplasia of the cementum, dentin, and alveolar bone has been proposed to be the main reasons for the exfoliation of teeth, while the extraordinarily complex intracellular mechanisms remain elusive. Dental pulp stem cells (DPSCs) have been demonstrated to successfully regenerate functional pulp-dentin-like tissue. Read More

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Nanotechnology, and scaffold implantation for the effective repair of injured organs: An overview on hard tissue engineering.

J Control Release 2021 Apr 3;333:391-417. Epub 2021 Apr 3.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

The tissue engineering of hard organs and tissues containing cartilage, teeth, and bones is a widely used and rapidly progressing field. One of the main features of hard organs and tissues is the mineralization of their extracellular matrices (ECM) to enable them to withstand pressure and weight. Recently, a variety of printing strategies have been developed to facilitate hard organ and tissue regeneration. Read More

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In Vitro Activity of Dental Pulp Stem Cells versus the Bone Marrow Stem Cells Cultured in Presence of a Bone Allograft.

J Long Term Eff Med Implants 2021 ;31(1):7-14

School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Introduction - Scaffolds and cells are two important components in bone tissue engineering. There is a gap of information about stem cell activity next to bone substitutes. Thus, the aim of this in vitro study was to compare the proliferation and differentiation of bone marrow stem cells (BMSCs) and dental pulp stem cells (DPSCs) in presence of freeze-dried bone allograft (FDBA). Read More

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January 2021

Novel mutation in the ALPL gene with a dominant negative effect in a Japanese family.

J Bone Miner Metab 2021 Apr 5. Epub 2021 Apr 5.

Department of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, N15W7, Kita-Ku, Sapporo, 060-8638, Japan.

Introduction: Hypophosphatasia (HPP) is caused by mutations in the ALPL gene encoding tissue nonspecific alkaline phosphatase (TNSALP) and inherited in either an autosomal recessive or autosomal dominant manner. It is characterized clinically by defective mineralization of bone, dental problems, and low serum ALP levels. In the current report, we demonstrate a novel mutation in the ALPL gene (c. Read More

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Consensus Recommendations for the Diagnosis and Management of X-Linked Hypophosphatemia in Belgium.

Front Endocrinol (Lausanne) 2021 19;12:641543. Epub 2021 Mar 19.

Department of Pediatric Nephrology, University Hospital Ghent, Ghent, Belgium.

X-linked hypophosphatemia (XLH) is the most common genetic form of hypophosphatemic rickets and osteomalacia. In this disease, mutations in the gene lead to elevated levels of the hormone fibroblast growth factor 23 (FGF23), resulting in renal phosphate wasting and impaired skeletal and dental mineralization. Recently, international guidelines for the diagnosis and treatment of this condition have been published. Read More

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Hesperidin Promotes Osteogenesis and Modulates Collagen Matrix Organization and Mineralization In Vitro and In Vivo.

Int J Mol Sci 2021 Mar 22;22(6). Epub 2021 Mar 22.

Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USA.

This study evaluated the direct effect of a phytochemical, hesperidin, on pre-osteoblast cell function as well as osteogenesis and collagen matrix quality, as there is little known about hesperidin's influence in mineralized tissue formation and regeneration. Hesperidin was added to a culture of MC3T3-E1 cells at various concentrations. Cell proliferation, viability, osteogenic gene expression and deposited collagen matrix analyses were performed. Read More

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Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications.

Int J Mol Sci 2021 Mar 12;22(5). Epub 2021 Mar 12.

The Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv 6997801, Israel.

Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM). Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity. Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer. Read More

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Effects of pre-mixed hydraulic calcium silicate putties on osteogenic differentiation of human dental pulp stem cells in vitro.

J Dent 2021 Mar 30;108:103653. Epub 2021 Mar 30.

Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA. Electronic address:

Objectives: An experimental tricalcium silicate and dicalcium silicate-containing endodontic putty has been designed to overcome the issue of reduced shelf life after exposure to atmospheric moisture during repeated opening of the container for clinical retrieval. The present study examined the effects of this experimental hydraulic putty on the mineralogenic characteristics of osteogenic lineage-committed human dental pulp stem cells (hDPSCs), by comparing the cellular responses with a commercially available putty (EndoSequence BC RRM Putty).

Methods: The osteogenic potential of hDPSCs that had been exposed to the putties was examined using quantitative reverse-transcription polymerase chain reaction for osteogenic gene expressions and western blot for osteogenic protein expressions. Read More

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Biomimetic oligopeptide formed enamel-like tissue and dentin tubule occlusion via mineralization for dentin hypersensitivity treatment.

J Appl Biomater Funct Mater 2021 Jan-Dec;19:22808000211005384

Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.

Objective: Dentin hypersensitivity (DH) is a common oral disease with approximately 41.9% prevalence. Reconstruction of dental hard tissues is the preferred treatment for relieving DH. Read More

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DNMT1 Inhibitor Restores RUNX2 Expression and Mineralization in Periodontal Ligament Cells.

DNA Cell Biol 2021 Mar 22. Epub 2021 Mar 22.

Health Science Institute, School of Dentistry, Paulista University-UNIP, São Paulo, Brazil.

Periodontal ligament cells (PDLCs) have well documented osteogenic potential; however, this commitment can be highly heterogenous, limiting their applications in tissue regeneration. In this study, we use PDLC populations characterized by high and low osteogenic potential (h-PDLCs and l-PDLCs, respectively) to identify possible sources of such heterogeneity and to investigate whether the osteogenic differentiation can be enhanced by epigenetic modulation. In h-PDLCs, low basal expression levels of pluripotency markers (, ), DNA methyltransferases (, ), and enzymes involved in active DNA demethylation (, ) were prerequisite to high osteogenic potential. Read More

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TiO Nanonetwork on Rough Ti Enhanced Osteogenesis In Vitro and In Vivo.

W E Yang H H Huang

J Dent Res 2021 Mar 20:220345211001017. Epub 2021 Mar 20.

Department of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan.

The objective in this study was to enhance osteogenic responses (in vitro and in vivo) to roughened titanium (Ti) dental implants through the formation of superhydrophilic TiO nanonetwork surface structure. Sandblasting and acid etching (SLA) was used to roughen the Ti surface. An electrochemical anodization process was then used to form a superhydrophilic TiO nanonetwork on the SLA Ti surfaces. Read More

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Mechanical Biocompatibility, Osteogenic Activity, and Antibacterial Efficacy of Calcium Silicate-Zirconia Biocomposites.

ACS Omega 2021 Mar 4;6(10):7106-7118. Epub 2021 Mar 4.

Institute of Oral Science, Chung Shan Medical University, Taichung 402, Taiwan.

Zirconia ceramics with high mechanical properties have been used as a load-bearing implant in the dental and orthopedic surgery. However, poor bone bonding properties and high elastic modulus remain a challenge. Calcium silicate (CaSi)-based ceramic can foster osteoblast adhesion, growth, and differentiation and facilitate bone ingrowth. Read More

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Abnormalities in Tooth Formation after Early Bisphosphonate Treatment in Children with Osteogenesis Imperfecta.

Calcif Tissue Int 2021 Mar 20. Epub 2021 Mar 20.

Department of Dental Medicine, Division of Orthodontics and Pediatric Dentistry, Karolinska Institutet, POB 4046, 141 04, Huddinge, Sweden.

Treatment with intravenous bisphosphonate (BP) in children and adolescents with osteogenesis imperfecta (OI) started in Sweden in 1991. No human studies on the role of BP therapy in development of disturbances in tooth mineralization or tooth morphology have been published. The study cohort comprised 219 individuals who were divided into four groups: group 1, BP treatment onset before 2 years of age (n = 22); group 2, BP treatment onset between 2 and 6 years of age (n = 20); group 3, BP treatment onset between 6 and 10 years of age (n = 13); and a control group of patients with OI who had not received BP therapy (n = 164). Read More

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Evaluating the potential of an amelogenin-derived peptide in tertiary dentin formation.

Regen Biomater 2021 Mar 13;8(2):rbab004. Epub 2021 Mar 13.

State Key Laboratory of Oral Diseases, Department of Cariology and Endodontics, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Several novel biomaterials have been developed for dental pulp capping by inducing tertiary dentin formation. The aim of this study was to evaluate the effect of QP5, an amelogenin-based peptide, on the mineralization of dental pulp cells (DPCs) and . The cell viability of human DPCs (hDPCs) after treatment with QP5 was determined using the Cell Counting Kit-8 (CCK-8). Read More

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Inhibition of osteoclast activities by SCPC bioceramic promotes osteoblast-mediated graft resorption and osteogenic differentiation.

J Biomed Mater Res A 2021 Mar 17. Epub 2021 Mar 17.

Periodontology and Implant Dentistry, The NYU College of Dentistry, New York, New York, USA.

Maximizing vital bone in a grafted site is dependent on a number of factors. These include resorption or turnover of the graft material, stimulation of bone formation pathway without a need for biological molecules added to the site and inhibition of cellular activities that compromise the mineralization of new bone matrix. In the present study, the dissolution profile of silica-calcium phosphate composite (SCPC) in physiological solution was measured and the data were fed to (ANN-NARX) prediction model to predict the time required for complete dissolution. Read More

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Effect of rosmarinic acid on differentiation and mineralization of MC3T3-E1 osteoblastic cells on titanium surface.

Anim Cells Syst (Seoul) 2021 Feb 18;25(1):46-55. Epub 2021 Feb 18.

Department of Dental Hygiene, College of Health Science, Youngsan University, Yangsan, Korea.

Titanium (Ti) is a widely used biomaterial for dental implants because of its outstanding biocompatibility for hard tissues. Osseointegration, the interaction between implanted biomaterials and living cells in bone, is essential for successful implantation. Rosmarinic acid (RA) is a plant-derived phytochemical with low toxicity and side effects and has various effects that can be applied as a therapeutic substance. Read More

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February 2021

Pre-conditioning of Mesenchymal Stem Cells with Piper longum L. augments osteogenic differentiation.

J Ethnopharmacol 2021 Jun 8;273:113999. Epub 2021 Mar 8.

Regenerative Medicine Laboratory, Dr. D. Y. Patil Dental College and Hospital, Pune, 411018, India.

Ethnopharmacological Relevance: The Indian Traditional Medicine, Ayurveda prescribes Piper longum L. popularly known as Long Pepper (Pippali) for the treatment of inflammatory and degenerative diseases. Therapeutic benefits of Piper longum L. Read More

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Bone quality analysis of jaw bones in individuals with type 2 diabetes mellitus-post mortem anatomical and microstructural evaluation.

Clin Oral Investig 2021 Mar 11. Epub 2021 Mar 11.

Laboratory for Anthropology and Skeletal Biology, Institute for Anatomy, School of Medicine, University of Belgrade, Dr Subotica starijeg 4, Belgrade, 11000, Serbia.

Objectives: With the higher risk of dental implant failure with type 2 diabetes mellitus (T2DM), there is a need to characterize the jaw bones in those individuals. The aim of this post mortem study was to compare jaw bone quality of individuals with T2DM to healthy controls.

Material And Methods: Bone cores from the edentulous lower first molar region and the region of mandibular angle were collected from male individuals with T2DM (n = 10, 70. Read More

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Constitutive activation of β-catenin in odontoblasts induces aberrant pulp calcification in mouse incisors.

J Mol Histol 2021 Mar 10. Epub 2021 Mar 10.

Department of Endodontics, School and Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji University, No.399 Yanchang Road, Shanghai, 200072, China.

During dentin formation, odontoblast polarization ensures that odontoblasts directionally secrete dentin matrix protein, leading to tubular dentin formation; however, little is known about the major features and regulatory mechanisms of odontoblast polarization. In a study of epithelial cell polarization, β-catenin was shown to serve as a structural component of cadherin-based adherens junctions to initiate cell polarity. However, the role of β-catenin in odontoblast polarization has not been well investigated. Read More

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Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue.

Restor Dent Endod 2021 Feb 2;46(1):e1. Epub 2020 Dec 2.

Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Canada.

Objectives: The aim of this study was to evaluate the dystrophic mineralization deposits from 3 calcium silicate-based cements (Micro-Mega mineral trioxide aggregate [MM-MTA], Biodentine [BD], and EndoSequence Root Repair Material [ESRRM] putty) over time after subcutaneous implantation into rats.

Materials And Methods: Forty-five silicon tubes containing the tested materials and 15 empty tubes (serving as a control group) were subcutaneously implanted into the backs of 15 Wistar rats. At 1, 4, and 8 weeks after implantation, the animals were euthanized ( = 5 animals/group), and the silicon tubes were removed with the surrounding tissues. Read More

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February 2021