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Identification of novel antihypertensive peptides from wine lees hydrolysate.

Food Chem 2021 Jul 24;366:130690. Epub 2021 Jul 24.

Universitat Rovira i Virgili, Department of Biochemistry and Biotechnology, Nutrigenomics Research Group, 43007 Tarragona, Spain. Electronic address:

Enzymatic-assisted extraction using Flavourzyme® has been demonstrated to be a useful methodology to obtain wine lees (WL) enriched in phenolic compounds and with enhanced antihypertensive activity. Nevertheless, taking into account that Flavourzyme® possess proteolytic activity, the release of bioactive peptides should not be ruled out. In this study, we investigate the presence of antihypertensive peptides in the WL hydrolysate. Read More

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mTORC2-mediated direct phosphorylation regulates YAP activity promoting glioblastoma growth and invasive characteristics.

Neoplasia 2021 Jul 31;23(9):951-965. Epub 2021 Jul 31.

Departments of Medicine; Jonnson Comprehensive Cancer Center; Molecular Biology Institute, University of California-Los Angeles, CL; Department of Research & Development, Greater Los Angeles Veterans Affairs Healthcare System, Los Angeles, CL. Electronic address:

The Hippo and mTOR signaling cascades are major regulators of cell growth and division. Aberrant regulation of these pathways has been demonstrated to contribute to gliomagenesis and result in enhanced glioblastoma proliferation and invasive characteristics. Several crosstalk mechanisms have been described between these two pathways, although a complete picture of these signaling interactions is lacking and is required for effective therapeutic targeting. Read More

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Regulation of the redox signaling and inflammation by Terminalia myriocarpa leaves and the predictive interactions of its major metabolites with iNOS and NF-ĸB.

J Ethnopharmacol 2021 Jul 31:114459. Epub 2021 Jul 31.

Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, E32004, Ourense, Spain. Electronic address:

Ethnopharmacological Relevance: The present study was designed to investigate the redox quenching and anti-inflammatory potentials of ethanolic leaf extract of Terminalia myriocarpa Van Heurck & Müller (ETM), inspired by the reported antioxidant potential of the plant bark and the anti-edema effect of the same genus.

Materials And Methods: HPLC-DAD dereplication study was conducted to detect various secondary metabolites. In-vitro DPPH free radical scavenging assay, nitric oxide scavenging assay, Fe ion chelating ability assay and reducing power assay were conducted to evaluate the redox quenching capacity. Read More

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Reciprocal allostery arising from a bi-enzyme assembly controls aromatic amino acid biosynthesis in Prevotella nigrescens.

J Biol Chem 2021 Jul 31:101038. Epub 2021 Jul 31.

Maurice Wilkins Centre, Ferrier Research Institute, Victoria University of Wellington, Wellington 6140, New Zealand. Electronic address:

Modular protein assembly has been widely reported as a mechanism for constructing allosteric machinery. Recently, a distinctive allosteric system has been identified in a bi-enzyme assembly comprising a 3-deoxy-D-arabino heptulosonate-7-phosphate synthase (DAH7PS) and chorismate mutase (CM). These enzymes catalyze the first and branch point reactions of aromatic amino acid biosynthesis in the bacterium Prevotella nigrescens (PniDAH7PS), respectively. Read More

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Electrochemical α-Arylation of Ketones via Anodic Oxidation of In Situ Generated Silyl Enol Ethers.

J Org Chem 2021 Aug 3. Epub 2021 Aug 3.

Institute of Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.

An electrochemical procedure for the α-arylation of ketones has been developed. The method is based on the generation and one-pot anodic oxidation of silyl enol ethers in the presence of the arene. This strategy avoids isolation of the silyl enol intermediate and the utilization of external supporting electrolytes. Read More

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Fimepinostat (CUDC-907) in patients with relapsed/refractory diffuse large B cell and high-grade B-cell lymphoma: report of a phase 2 trial and exploratory biomarker analyses.

Br J Haematol 2021 Aug 2. Epub 2021 Aug 2.

MD Anderson Cancer Center, Houston, TX, USA.

Fimepinostat (CUDC-907), a first-in-class oral small-molecule inhibitor of histone deacetylase and phosphatidylinositol 3-kinase, demonstrated efficacy in a phase 1 study of patients with relapsed/refractory (R/R) diffuse large and high-grade B-cell lymphomas (DLBCL/HGBL), particularly those with increased MYC protein expression and/or MYC gene rearrangement/copy number gain (MYC-altered disease). Therefore, a phase 2 study of fimepinostat was conducted in this patient population with 66 eligible patients treated. The primary end-point of overall response (OR) rate for patients with MYC-IHC ≥40% (n = 46) was 15%. Read More

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Behavior of Iron and Ferritin After Bariatric Surgery in Patients With and Without Hepatic Steatosis.

Obes Surg 2021 Aug 3. Epub 2021 Aug 3.

Departamento de Ciências da Vida, Universidade do Estado da Bahia - UNEB, Rua Silveira Martins, 2555, Cabula, Salvador, BA, CEP: 41.150-000, Brazil.

Background: Iron deficiency and hepatic steatosis are common in bariatric surgery patients. Steatosis can falsely elevate ferritin values even in presence of iron deficiency. This study aims to assess the influence of hepatic steatosis on iron deficiency and replacement therapy after bariatric surgery. Read More

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Outcome of urgent desensitization in sensitized heart transplant recipients.

J Formos Med Assoc 2021 Jul 30. Epub 2021 Jul 30.

Department of Cardiac Surgery, National Taiwan University Hospital, School of Medicine, National Taiwan University, Taipei, Taiwan. Electronic address:

Background/purpose: Sensitization, the presence of preformed anti-human antibody in recipients, restricts access to ABO-compatible donors in heart transplant. Desensitization therapy works by reducing preformed antibodies to increase the chances of a negative crossmatch or permit safe transplantation across positive crossmatch. There is no consensus regarding the desensitization protocol in cardiac patients, and the outcome of desensitization remains under debate. Read More

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Anterior and apical samplings during transperineal image-guided prostate biopsy.

Urol Oncol 2021 Jul 31. Epub 2021 Jul 31.

Department of Urology, Tel-Aviv Sourasky Medical Center, Sackler School of Medicine, Tel-Aviv University, Tel Aviv-Yafo, Israel.

Introduction: Concurrent systematic biopsies during image-guided targeted biopsies of the prostate were found to improve the detection rate of clinically significant prostate cancer (CSPC). However, these biopsies do not routinely include anterior or apical sampling. We aimed to evaluate the significance of anterior and apical samplings during combined biopsies. Read More

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Clinical factors associated with peripheral artery disease in patients with documented coronary artery disease: A post hoc analysis of the COMPASS trial.

Atherosclerosis 2021 Jul 11. Epub 2021 Jul 11.

University of Alberta, Mazankowski Heart Institute, 8440 112, St NW Edmonton, Alberta, T6G 2B7, Canada. Electronic address:

Background And Aims: Patients with coronary artery disease (CAD) who also have peripheral artery disease (PAD) are at high risk of subsequent cardiovascular events and mortality. Despite this, PAD in patients with CAD often remains undiagnosed. The objective of this analysis was to assess clinical factors that predict the presence of PAD in patient with documented CAD who also have PAD. Read More

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Effect of the polymorphism (rs11548193) on plasma branched-chain amino acid concentrations after dietary intervention in subjects with obesity and insulin resistance.

Br J Nutr 2021 Aug 3:1-30. Epub 2021 Aug 3.

Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México, México.

Background & Aims: Branched-chain amino acids (BCAAs) are considered markers of insulin resistance (IR) in subjects with obesity. In this study, we evaluated whether the presence of a single nucleotide polymorphism (SNP) of the branched-chain aminotransferase 2 (BCAT2) gene can modify the effect of a dietary intervention (DI) on the plasma concentration of BCAAs in subjects with obesity and IR.

Methods: A prospective cohort study of adult subjects with obesity, body mass index (BMI) ≥ 30 kg/m2, IR (HOMA-IR ≥ 2. Read More

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Do Ultrasound Patterns and Clinical Parameters Inform the Probability of Thyroid Cancer Predicted by Molecular Testing in Nodules with Indeterminate Cytology?

Thyroid 2021 Aug 3. Epub 2021 Aug 3.

University of Pittsburgh, Surgery, Division of Endocrine Surgery, 101 Kaufmann, 3471 Fifth Avenue, Pittsburgh, United States, 15213.

: Molecular testing (MT) is commonly used to refine cancer probability in thyroid nodules with indeterminate cytology. Whether or not ultrasound (US) patterns and clinical parameters can further inform the risk of thyroid cancer in nodules predicted to be positive or negative by MT remains unknown. Aim: To test if clinical parameters, including patient age, gender, nodule size (by US), Bethesda category (III, IV, V), US pattern (American Thyroid Association [ATA] vs American College of Radiology TI-RADS systems), radiation exposure, or family history of thyroid cancer can modify the probability of thyroid cancer or NIFTP predicted by MT. Read More

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First report of Phytoplasma cynodontis (16SrXIV-A subgroup) associated with cauliflower phyllody and flat stem in India.

Plant Dis 2021 Aug 2. Epub 2021 Aug 2.

Acharya NG Ranga Agricultural University Sri Venkateswara Agricultural College, 72923, Plant Pathology, Tirupati, Andhra Pradesh, India;

Symptoms of suspected phytoplasma infection were observed in cauliflower ( var. ) (cultivar NS60N) at Integrated Farming System Research Station, Trivandrum, Kerala, India (08o28'28"N, 76o57'47"E) in April-2021. The disease incidence was recorded up to 10% in different fields. Read More

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Can commercial automated immunoassays be utilized to predict neutralizing antibodies after SARS-CoV-2 infection? A comparative study between three different assays.

Front Biosci (Landmark Ed) 2021 07;26(7):198-206

Biomedical Research Center, Qatar University, 2713 Doha, Qatar.

: High-throughput assays that can infer neutralizing activity against SARS-CoV-2 are of great importance for assessing the immunity induced by natural infection and COVID-19 vaccines. We aimed to evaluate the performance and degree of correlation of three fully automated anti-SARS-CoV-2 immunoassays with neutralization activity using a surrogate virus-neutralizing test (sVNT) from GenScript, targeting the receptor-binding domain. : 110 sera collected from PCR-confirmed asymptomatic COVID-19 individuals were tested for neutralizing antibodies (nAbs) using the sVNT. Read More

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Disease outcomes and biomarkers of progression in smouldering Waldenström macroglobulinaemia.

Br J Haematol 2021 Aug 2. Epub 2021 Aug 2.

Division of Hematology, Mayo Clinic, Rochester, MN, USA.

Patients with asymptomatic/smouldering Waldenström macroglobulinaemia (SWM) have a variable risk of progression to active WM. Our study evaluated 143 patients with SWM consecutively seen between January 1996 and December 2013. With a median [95% confidence interval (CI)] follow-up of 9·5 [8·1-11·5] years, the cumulative rate of progression was 11% at 1 year, 38% at 3 years and 55% at 5 years. Read More

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Contradictory tendency of As(V) releasing from Fe-As complexes: Influence of organic and inorganic anions.

Chemosphere 2021 Jul 22;286(Pt 2):131469. Epub 2021 Jul 22.

Institute of Agricultural Environment and Sustainable Development, Chinese Academy of Agriculture Sciences, Beijing, 100081, China. Electronic address:

The strong ability of ferrihydrite and its aged minerals for fixing arsenate is a key factor in remediating arsenate-polluted environments. It is therefore crucial to clarify the stability of Fe-As complexes and the release conditions for As(V). The As(V) release amount was evaluated and compared in the presence of six representative anions, namely, phosphate, silicate, sulfate, inositol hexaphosphate, citrate, and oxalate. Read More

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Microplastics deteriorate the removal efficiency of antibiotic resistance genes during aerobic sludge digestion.

Sci Total Environ 2021 Jul 30;798:149344. Epub 2021 Jul 30.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia. Electronic address:

Sludge from wastewater treatment plants (WWTPs) is considered to be reservoirs of antibiotic resistance genes (ARGs), which can be efficiently removed by sludge treatment processes, e.g., aerobic sludge digestion. Read More

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The role of redundant verbal labels in 8- and 10-month-olds' working memory.

Infant Behav Dev 2021 Jul 30;64:101617. Epub 2021 Jul 30.

University of Massachusetts Boston, United States.

Verbal labels have been shown to help preverbal infants' performance on various cognitive tasks, such as categorization. Redundant labels also aid adults' visual working memory (WM), but it is not known if this linguistic benefit extends to preverbal infants' WM. In two eye-tracking studies, we tested whether 8- and 10-month-old infants' WM performance would improve with the presence of redundant labels in a Delayed Match Retrieval (DMR) paradigm that tested infants' WM for object-location bindings. Read More

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Immune environment of the brain in schizophrenia and during the psychotic episode: a human post-mortem study.

Brain Behav Immun 2021 Jul 30. Epub 2021 Jul 30.

Clinical Neurosciences, Clinical and Experimental Sciences, Faculty of Medicine University of Southampton, Southampton, UK. Electronic address:

A causal relationship between immune dysregulation and schizophrenia has been supported by genome-wide association studies and epidemiological evidence. It remains unclear to what extent the brain immune environment is implicated in this hypothesis. We investigated the immunophenotype of microglia and the presence of perivascular macrophages and T lymphocytes in post-mortem brain tissue. Read More

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Pterin-photosensitization of thymine under anaerobic conditions in the presence of guanine.

Free Radic Biol Med 2021 Jul 30. Epub 2021 Jul 30.

Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), CCT La Plata-CONICET, Diagonal 113 y 64, 1900, La Plata, Argentina. Electronic address:

Pterin (Ptr) is a model photosensitizer that acts mainly through type I mechanism and is able to photoinduce the one-electron oxidation of purine and pyrimidine nucleobases. However, under anaerobic conditions Ptr reacts with thymine (T) to form photoadducts (Ptr-T) but does not lead to the photodegradation of guanine (G), which is the nucleobase with the lowest ionization potential. Accordingly, G is thermodynamically able to reduce the radicals of the other nucleobases and has been described in this sense as the "hole sink" of the DNA double helix. Read More

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The scaffold protein XRCC1 stabilizes the formation of polβ/gap DNA and ligase IIIα/nick DNA complexes in base excision repair.

J Biol Chem 2021 Jul 30:101025. Epub 2021 Jul 30.

Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA. Electronic address:

The base excision repair (BER) pathway involves gap filling by DNA polymerase (pol) β and subsequent nick sealing by ligase IIIα. X-ray cross-complementing protein 1 (XRCC1), a non-enzymatic scaffold protein, assembles multi-protein complexes, although the mechanism by which XRCC1 orchestrates the final steps of coordinated BER remains incompletely defined. Here, using a combination of biochemical and biophysical approaches, we revealed that the polβ/XRCC1 complex increases the processivity of BER reactions after correct nucleotide insertion into gaps in DNA and enhances the handoff of nicked repair products to the final ligation step. Read More

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Prognostic and clinicopathological significance of TMEFF2, SMOC-2, and SOX17 expression in endometrial carcinoma.

Exp Mol Pathol 2021 Jul 30:104670. Epub 2021 Jul 30.

Department of Gynecology and Obstetrics, Faculty of Medicine, Zagazig University, Egypt.

Background there is a need for novel biomarkers and targeting therapies for predicting Endometrial carcinoma (EC) progression and recurrence. TMEFF2 is a gene that was found to play a role in EMT. SMOC-2 is expressed in embryogenesis and it was identified as a recent stem cell-related gene that has a role in cancer progression. Read More

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High-throughput Interpretation of Killer-cell Immunoglobulin-like Receptor Short-read Sequencing Data with PING.

PLoS Comput Biol 2021 Aug 2;17(8):e1008904. Epub 2021 Aug 2.

UCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, San Francisco, California, United States of America.

The killer-cell immunoglobulin-like receptor (KIR) complex on chromosome 19 encodes receptors that modulate the activity of natural killer cells, and variation in these genes has been linked to infectious and autoimmune disease, as well as having bearing on pregnancy and transplant outcomes. The medical relevance and high variability of KIR genes makes short-read sequencing an attractive technology for interrogating the region, providing a high-throughput, high-fidelity sequencing method that is cost-effective. However, because this gene complex is characterized by extensive nucleotide polymorphism, structural variation including gene fusions and deletions, and a high level of homology between genes, its interrogation at high resolution has been thwarted by bioinformatic challenges, with most studies limited to examining presence or absence of specific genes. Read More

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Risk Factors and Protective Immunity Against Rabies in Unvaccinated Butchers Working at Dog Slaughterhouses in Northern Vietnam.

Am J Trop Med Hyg 2021 Aug 2. Epub 2021 Aug 2.

Microbiology Department, Faculty of Medicine, Oita University, Oita, Japan.

Vietnam is a rabies-endemic country where eating dog meat is customary. However, the risks of rabies transmission to dog slaughtering and processing workers have not been identified. This study aimed to determine the rabies neutralizing antibody (NTA) and risk factors in dog slaughterers to propose appropriate intervention methods for this occupational group. Read More

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SPM Receptor Expression and Localization in Irradiated Salivary Glands.

J Histochem Cytochem 2021 Aug;69(8):523-534

Department of Otolaryngology-Head and Neck Surgery, University of Missouri, Columbia, Missouri.

Radiation therapy-mediated salivary gland destruction is characterized by increased inflammatory cell infiltration and fibrosis, both of which ultimately lead to salivary gland hypofunction. However, current treatments (e.g. Read More

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Associations between varied susceptibilities of PfATP4 inhibitors and genotypes in Ugandan isolates.

Antimicrob Agents Chemother 2021 Aug 2:AAC0077121. Epub 2021 Aug 2.

Dept. of Medicine, University of California, San Francisco, CA, USA.

Among novel compounds under recent investigation as potential new antimalarial drugs are three independently developed inhibitors of the P-type ATPase (PfATP4): KAE609 (cipargamin), PA92, and SJ733. We assessed susceptibilities to these compounds of 374 fresh isolates collected in Tororo and Busia districts, Uganda from 2016-2019. Median ICs were 65 nM for SJ733, 9. Read More

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A Vaccine to Prevent Egg Layer Peritonitis in Chickens.

Avian Dis 2021 Mar;65(1):198-204

Epitopix, LLC, Willmar, MN 56201.

A series of studies was undertaken in specific-pathogen-free white leghorn chickens for the development of a chicken model of avian pathogenic (APEC) peritonitis. Once established, this model was then used to measure the effectiveness of a siderophore receptor and porin proteins (SRP) APEC vaccine. Initially, five pilot studies were performed to compare the serotype, challenge route, and dose of inoculum that resulted in pathologies characteristic of the peritonitis observed in commercial layer facilities, such as widespread organ infection, atrophy, discoloration, corrugation of yolk sacs, and the presence of caseous exudate. Read More

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Serial assessment of inflammatory parameters for prediction of septic complications following surgery for colorectal endometriosis : A descriptive, retrospective study.

Wien Klin Wochenschr 2021 Aug 2. Epub 2021 Aug 2.

Department of Gynecology, Center for Endometriosis, Hospital St. John of God Vienna, Vienna, Austria.

Purpose: To assess whether C‑reactive protein (CRP), white blood cell count (WBC) and body temperature changes are suitable parameters for the early detection of septic complications following resection of colorectal deep endometriosis (DE).

Methods: Retrospective data analysis of CRP, WBC and body temperature courses following colorectal surgery for DE at a tertiary referral center for endometriosis.

Results: Out of 183 surgeries performed, 10 major surgical complications were observed, including 4 anastomotic leakages (AL 2%) and 2 rectovaginal fistulae (RVF 1%). Read More

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Gated Dissipative Dynamic Artificial Photosynthetic Model Systems.

J Am Chem Soc 2021 Aug 2. Epub 2021 Aug 2.

Institute of Chemistry, The Minerva Center for Bio-hybrid Complex Systems, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Gated dissipative artificial photosynthetic systems modeling dynamically modulated environmental effects on the photosynthetic apparatus are presented. Two photochemical systems composed of a supramolecular duplex scaffold, a photosensitizer-functionalized strand (photosensitizer is Zn(II)protoporphyrin IX, Zn(II)PPIX, or pyrene), an electron acceptor bipyridinium (V)-modified strand, and a nicking enzyme (Nt.BbvCI) act as functional assemblies driving transient photosynthetic-like processes. Read More

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Butyryl/Caproyl-CoA:Acetate CoA-Transferase: Cloning, Expression and Characterization of the Key Enzyme Involved in Medium-Chain Fatty Acid Biosynthesis.

Biosci Rep 2021 Aug 2. Epub 2021 Aug 2.

The Chinese Academy of Sciences, Zigong, China.

Coenzyme A transferases (CoATs) are important enzymes involved in carbon chain elongation, contributing to medium-chain fatty acid (MCFA) biosynthesis. For example, butyryl-CoA:acetate CoA transferase (BCoAT) is responsible for the final step of butyrate synthesis from butyryl-CoA. However, little is known about caproyl-CoA:acetate CoA-transferase (CCoAT), which is responsible for the final step of caproate synthesis from caproyl-CoA. Read More

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