115 results match your criteria demonstrate eb1

The Murine MHC Class II Super Enhancer Contains a Functionally Redundant CTCF-Binding Component and a Novel Element Critical for Maximal Expression.

J Immunol 2021 05 16;206(9):2221-2232. Epub 2021 Apr 16.

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA

In both humans and mice, CTCF-binding elements form a series of interacting loops across the MHC class II (MHC-II) locus, and CTCF is required for maximal MHC-II gene expression. In humans, a CTCF-bound chromatin insulator termed and a super enhancer (SE) situated in the intergenic region between and play critical roles in regulating MHC-II expression. In this study, we identify a similar SE, termed located between and of the mouse that contains a CTCF site () and a novel region of high histone H3K27 acetylation. Read More

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Facile Synthesis of a Color-Tunable Microcrystal Phosphor for Anti-Counterfeit Applications.

ACS Omega 2020 Dec 7;5(50):32420-32425. Epub 2020 Dec 7.

Department of Chemical & Biomolecular Engineering, North Carolina State University, 911 Partners Way, Engineering Building I (EB1), Raleigh, North Carolina 27606 United States.

Developing luminescent materials with tunable emission colors provides exciting opportunities for application in the display, anti-counterfeiting, and optical sensors. Here, we report a convenient, versatile approach to synthesize color-tunable, up/down-conversion luminescence in an inorganic host material. The emission color can be tuned by varying the excitation wavelength, allowing dynamic color tuning in the visible spectrum. Read More

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December 2020

Electrostatic effects in saturation of membrane binding of cationic cell-penetrating peptide.

Eur Biophys J 2021 Jan 27;50(1):15-23. Epub 2020 Nov 27.

Saint-Petersburg Clinical Scientific and Practical Center of Specialized Types of Medical Care (Oncological), St. Petersburg, Russia.

Membrane-active peptides that demonstrate cell-penetrating, antimicrobial or cytotoxic functions are diverse in their amino acid sequences, but share common physicochemical features like short length, amphipathic conformation in membrane environment and high net charge. Nonspecific electrostatic interactions of basic peptide residues with anionic membrane lipids play a crucial role in the initial binding of such peptides to plasma membranes of bacterial and mammalian cells. At the same time, a number of membrane-active peptides functions when they are localized at high concentrations on the lipid membranes. Read More

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January 2021

3D Particle Tracking for Noninvasive In Vivo Analysis of Synaptic Microtubule Dynamics in Dendrites and Neuromuscular Junctions of Drosophila.

J Vis Exp 2020 05 12(159). Epub 2020 May 12.

Blavatnik Institute of Cell Biology and Program in Neuroscience, Harvard Medical School;

Microtubules (MTs) play critical roles in neuronal development, but many questions remain about the molecular mechanisms of their regulation and function. Furthermore, despite progress in understanding postsynaptic MTs, much less is known about the contributions of presynaptic MTs to neuronal morphogenesis. In particular, studies of in vivo MT dynamics in Drosophila sensory dendrites yielded significant insights into polymer-level behavior. Read More

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Collective effects of XMAP215, EB1, CLASP2, and MCAK lead to robust microtubule treadmilling.

Proc Natl Acad Sci U S A 2020 06 26;117(23):12847-12855. Epub 2020 May 26.

Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37240;

Microtubule network remodeling is essential for fundamental cellular processes including cell division, differentiation, and motility. Microtubules are active biological polymers whose ends stochastically and independently switch between phases of growth and shrinkage. Microtubule treadmilling, in which the microtubule plus end grows while the minus end shrinks, is observed in cells; however, the underlying mechanisms are not known. Read More

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Microtubule polymerization state and clathrin-dependent internalization regulate dynamics of cardiac potassium channel: Microtubule and clathrin control of K1.5 channel.

J Mol Cell Cardiol 2020 07 20;144:127-139. Epub 2020 May 20.

INSERM UMRS1166, ICAN - Institute of CardioMetabolism and Nutrition, Sorbonne Université, Paris, France. Electronic address:

Ion channel trafficking powerfully influences cardiac electrical activity as it regulates the number of available channels at the plasma membrane. Studies have largely focused on identifying the molecular determinants of the trafficking of the atria-specific K1.5 channel, the molecular basis of the ultra-rapid delayed rectifier current I. Read More

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Site-directed MT1-MMP trafficking and surface insertion regulate AChR clustering and remodeling at developing NMJs.

Elife 2020 03 24;9. Epub 2020 Mar 24.

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

At vertebrate neuromuscular junctions (NMJs), the synaptic basal lamina contains different extracellular matrix (ECM) proteins and synaptogenic factors that induce and maintain synaptic specializations. Here, we report that podosome-like structures (PLSs) induced by ubiquitous ECM proteins regulate the formation and remodeling of acetylcholine receptor (AChR) clusters via focal ECM degradation. Mechanistically, ECM degradation is mediated by PLS-directed trafficking and surface insertion of membrane-type 1 matrix metalloproteinase (MT1-MMP) to AChR clusters through microtubule-capturing mechanisms. Read More

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Septin 2/6/7 complexes tune microtubule plus-end growth and EB1 binding in a concentration- and filament-dependent manner.

Mol Biol Cell 2019 11 2;30(23):2913-2928. Epub 2019 Oct 2.

Department of Biology, Drexel University, Philadelphia, PA 19104.

Septins (SEPTs) are filamentous guanosine-5'-triphosphate (GTP)-binding proteins, which affect microtubule (MT)-dependent functions including membrane trafficking and cell division, but their precise role in MT dynamics is poorly understood. Here, in vitro reconstitution of MT dynamics with SEPT2/6/7, the minimal subunits of septin heteromers, shows that SEPT2/6/7 has a biphasic concentration-dependent effect on MT growth. Lower concentrations of SEPT2/6/7 enhance MT plus-end growth and elongation, while higher and intermediate concentrations inhibit and pause plus-end growth, respectively. Read More

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November 2019

EB1- and EB2-dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasion.

FASEB J 2019 08 21;33(8):9434-9452. Epub 2019 May 21.

Program of Cellular and Molecular Biology, Universidad de Chile, Santiago, Chile.

Transient receptor potential melastatin 4 (TRPM4) is a Ca-activated nonselective cationic channel involved in a wide variety of physiologic and pathophysiological processes. Bioinformatics analyses of the primary sequence of TRPM4 allowed us to identify a putative motif for interaction with end-binding (EB) proteins, which are microtubule plus-end tracking proteins. Here, we provide novel data suggesting that TRPM4 interacts with EB proteins. Read More

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STIM1 Is Required for Remodeling of the Endoplasmic Reticulum and Microtubule Cytoskeleton in Steering Growth Cones.

J Neurosci 2019 06 25;39(26):5095-5114. Epub 2019 Apr 25.

School of Medicine,

The spatial and temporal regulation of calcium signaling in neuronal growth cones is essential for axon guidance. In growth cones, the endoplasmic reticulum (ER) is a significant source of calcium signals. However, it is not clear whether the ER is remodeled during motile events to localize calcium signals in steering growth cones. Read More

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DRG2 Deficiency Causes Impaired Microtubule Dynamics in HeLa Cells.

Mol Cells 2018 Dec 14;41(12):1045-1051. Epub 2018 Nov 14.

School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea.

The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (shDRG2) significantly reduced the growth rate of HeLa cells. Read More

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December 2018

GTP-binding facilitates EB1 recruitment onto microtubules by relieving its auto-inhibition.

Sci Rep 2018 06 28;8(1):9792. Epub 2018 Jun 28.

School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, CET Campus, Thiruvananthapuram, 695016, Kerala, India.

Microtubule plus end-binding protein, EB1 is a key regulator of microtubule dynamics. Auto-inhibitory interaction in EB1 has previously been shown to inhibit its ability to bind to microtubules and regulate microtubule dynamics. However, the factors that promote its microtubule regulatory activity by over-coming the auto-inhibition are less known. Read More

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EB1 contributes to microtubule bundling and organization, along with root growth, in .

Biol Open 2018 Aug 2;7(8). Epub 2018 Aug 2.

Department of Cell Biology, Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette Cedex, France

Microtubules are involved in plant development and adaptation to their environment, but the sustaining molecular mechanisms remain elusive. Microtubule-end-binding 1 (EB1) proteins participate in directional root growth in However, a connection to the underlying microtubule array has not been established yet. We show here that EB1 proteins contribute to the organization of cortical microtubules in growing epidermal plant cells, without significant modulation of microtubule dynamics. Read More

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EB1 traps STIM1 and regulates local store-operated Ca entry.

J Cell Biol 2018 06 15;217(6):1899-1900. Epub 2018 May 15.

State Key Laboratory of Membrane Biology, Institute of Zoology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, China.

STIM1 activates store-operated Ca entry when Ca in the endoplasmic reticulum (ER) is depleted. In this issue, Chang et al. (2018. Read More

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Structure-Function Relationship of the Bik1-Bim1 Complex.

Structure 2018 04 22;26(4):607-618.e4. Epub 2018 Mar 22.

Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institut, 5232 Villigen PSI, Switzerland; University of Basel, Biozentrum, 4056 Basel, Switzerland. Electronic address:

In budding yeast, the microtubule plus-end tracking proteins Bik1 (CLIP-170) and Bim1 (EB1) form a complex that interacts with partners involved in spindle positioning, including Stu2 and Kar9. Here, we show that the CAP-Gly and coiled-coil domains of Bik1 interact with the C-terminal ETF peptide of Bim1 and the C-terminal tail region of Stu2, respectively. The crystal structures of the CAP-Gly domain of Bik1 (Bik1CG) alone and in complex with an ETF peptide revealed unique, functionally relevant CAP-Gly elements, establishing Bik1CG as a specific C-terminal phenylalanine recognition domain. Read More

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Human CLASP2 specifically regulates microtubule catastrophe and rescue.

Mol Biol Cell 2018 05 22;29(10):1168-1177. Epub 2018 Mar 22.

Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37240.

Cytoplasmic linker-associated proteins (CLASPs) are microtubule-associated proteins essential for microtubule regulation in many cellular processes. However, the molecular mechanisms underlying CLASP activity are not understood. Here, we use purified protein components and total internal reflection fluorescence microscopy to investigate the effects of human CLASP2 on microtubule dynamics in vitro. Read More

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Structural and functional differences between porcine brain and budding yeast microtubules.

Cell Cycle 2018 30;17(3):278-287. Epub 2018 Jan 30.

d Molecular Biophysics and Integrated Bioimaging , Lawrence Berkeley National Laboratory , CA 94720 , USA.

The cytoskeleton of eukaryotic cells relies on microtubules to perform many essential functions. We have previously shown that, in spite of the overall conservation in sequence and structure of tubulin subunits across species, there are differences between mammalian and budding yeast microtubules with likely functional consequences for the cell. Here we expand our structural and function comparison of yeast and porcine microtubules to show different distribution of protofilament number in microtubules assembled in vitro from these two species. Read More

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September 2019

H- and Na- elicited rapid changes of the microtubule cytoskeleton in the biflagellated green alga .

Elife 2017 09 6;6. Epub 2017 Sep 6.

Department of Biological Sciences, Marquette University, Milwaukee, United States.

Although microtubules are known for dynamic instability, the dynamicity is considered to be tightly controlled to support a variety of cellular processes. Yet diverse evidence suggests that this is not applicable to , a biflagellate fresh water green alga, but intense autofluorescence from photosynthesis pigments has hindered the investigation. By expressing a bright fluorescent reporter protein at the endogenous level, we demonstrate in real time discreet sweeping changes in algal microtubules elicited by rises of intracellular H and Na. Read More

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September 2017

The Microtubule-Stabilizing Protein CLASP1 Associates with the Schizont Surface via Its Kinetochore-Binding Domain.

mSphere 2017 Jul-Aug;2(4). Epub 2017 Aug 23.

Institute for Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

is an apicomplexan parasite whose presence within the cytoplasm of a leukocyte induces cellular transformation and causes uncontrolled proliferation and clonal expansion of the infected cell. The intracellular schizont utilizes the host cell's own mitotic machinery to ensure its distribution to both daughter cells by associating closely with microtubules (MTs) and incorporating itself within the central spindle. We show that CLASP1, an MT-stabilizing protein that plays important roles in regulating kinetochore-MT attachment and central spindle positioning, is sequestered at the schizont surface. Read More

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Featherlight, Mechanically Robust Cellulose Ester Aerogels for Environmental Remediation.

ACS Omega 2017 Aug 8;2(8):4297-4305. Epub 2017 Aug 8.

Department of Chemical & Biomolecular Engineering, NC State University, 911 Partners Way, Engineering Building I (EB1), Raleigh, North Carolina 27695-7905, United States.

A unique combination of well-established synthesis procedures involving chemical cross-linking, careful solvent exchange to water, and subsequent freeze drying is used to produce ultralight (4.3 mg/mL) and highly porous (99.7%) cellulose diacetate (CDA) aerogels with honeycomb morphology. Read More

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Role of End Binding Protein-1 in endothelial permeability response to barrier-disruptive and barrier-enhancing agonists.

Cell Signal 2017 01 23;29:1-11. Epub 2016 Sep 23.

Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, IL 60637, United States. Electronic address:

Rapid changes in microtubule (MT) polymerization dynamics affect regional activity of small GTPases RhoA and Rac1, which play a key role in the regulation of actin cytoskeleton and endothelial cell (EC) permeability. This study tested the role of End Binding Protein-1 (EB1) in the mechanisms of increased and decreased EC permeability caused by thrombin and hepatocyte growth factor (HGF) and mediated by RhoA and Rac1 GTPases, respectively. Stimulation of human lung EC with thrombin inhibited peripheral MT growth, which was monitored by morphological and biochemical evaluation of peripheral MT and the levels of stabilized MT. Read More

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January 2017

A Force-Induced Directional Switch of a Molecular Motor Enables Parallel Microtubule Bundle Formation.

Cell 2016 Oct;167(2):539-552.e14

Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, 1030 Vienna, Austria; Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna, Austria; Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), Dr. Bohr-Gasse 7, 1030 Vienna, Austria; The Rockefeller University, 1230 York Avenue New York, NY 10065, USA. Electronic address:

Microtubule-organizing centers (MTOCs) nucleate microtubules that can grow autonomously in any direction. To generate bundles of parallel microtubules originating from a single MTOC, the growth of multiple microtubules needs to coordinated, but the underlying mechanism is unknown. Here, we show that a conserved two-component system consisting of the plus-end tracker EB1 and the minus-end-directed molecular motor Kinesin-14 is sufficient to promote parallel microtubule growth. Read More

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October 2016

Proto-Oncogenic Src Phosphorylates EB1 to Regulate the Microtubule-Focal Adhesion Crosstalk and Stimulate Cell Migration.

Theranostics 2016 12;6(12):2129-2140. Epub 2016 Sep 12.

State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, China.; Institute of Biomedical Sciences, College of Life Sciences, Key Laboratory of Animal Resistance Biology of Shandong Province, Key Laboratory of Molecular and Nano Probes of the Ministry of Education, Shandong Normal University, Jinan 250014, China.

Cell migration, a complex process critical for tumor progression and metastasis, requires a dynamic crosstalk between microtubules (MTs) and focal adhesions (FAs). However, the molecular mechanisms underlying this event remain elusive. Herein we identify the proto-oncogenic protein Src as an important player in the regulation of the MT-FA crosstalk. Read More

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October 2017

Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells.

Oncotarget 2016 Nov;7(45):72699-72715

Blokhin Russian Cancer Research Center, Moscow, Russia.

Actin microfilaments and microtubules are both highly dynamic cytoskeleton components implicated in a wide range of intracellular processes as well as cell-cell and cell-substrate interactions. The interactions of actin filaments with the microtubule system play an important role in the assembly and maintenance of 3D cell structure. Here we demonstrate that cytoplasmic actins are differentially distributed in relation to the microtubule system. Read More

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November 2016

Investigating the Responses of Human Epithelial Cells to Predatory Bacteria.

Sci Rep 2016 09 15;6:33485. Epub 2016 Sep 15.

Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology, 44919, South Korea.

One beguiling alternative to antibiotics for treating multi-drug resistant infections are Bdellovibrio-and-like-organisms (BALOs), predatory bacteria known to attack human pathogens. Consequently, in this study, the responses from four cell lines (three human and one mouse) were characterized during an exposure to different predatory bacteria, Bdellovibrio bacteriovorus HD100, Bacteriovorus BY1 and Bacteriovorax stolpii EB1. TNF-α levels were induced in Raw 264. Read More

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September 2016

Olesoxime favors oligodendrocyte differentiation through a functional interplay between mitochondria and microtubules.

Neuropharmacology 2016 12 13;111:293-303. Epub 2016 Sep 13.

Aix Marseille Univ, CNRS, IBDM, Marseille, France. Electronic address:

Multiple sclerosis (MS) is a neurodegenerative disease characterized by episodes of immune attacks and oligodendrocyte death leading to demyelination and progressive functional deficits. New therapeutic strategies are needed to stimulate the spontaneous regenerative process observed in some patients. Spontaneous myelin repair relies on the mobilization and differentiation of endogenous oligodendrocyte progenitors at the lesion site. Read More

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December 2016

FoxO regulates microtubule dynamics and polarity to promote dendrite branching in Drosophila sensory neurons.

Dev Biol 2016 10 18;418(1):40-54. Epub 2016 Aug 18.

Department of Neurosciences, Case Western Reserve University, Cleveland, OH 44106, USA. Electronic address:

The size and shape of dendrite arbors are defining features of neurons and critical determinants of neuronal function. The molecular mechanisms establishing arborization patterns during development are not well understood, though properly regulated microtubule (MT) dynamics and polarity are essential. We previously found that FoxO regulates axonal MTs, raising the question of whether it also regulates dendritic MTs and morphology. Read More

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October 2016

Endophilin B2 promotes inner mitochondrial membrane degradation by forming heterodimers with Endophilin B1 during mitophagy.

Sci Rep 2016 04 26;6:25153. Epub 2016 Apr 26.

State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

Mitochondrial sequestration by autophagosomes is a key step in mitophagy while the mechanisms mediating this process are not fully understood. It has been reported that Endophilin B1 (EB1) promotes mitochondrial sequestration by binding and shaping membrane. However, the role of EB1 homolog Endophilin B2 (EB2) in mitophagy remains unclear. Read More

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Developmental Nuclear Localization and Quantification of GFP-Tagged EB1c in Arabidopsis Root Using Light-Sheet Microscopy.

Front Plant Sci 2015 5;6:1187. Epub 2016 Jan 5.

Department of Cell Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc Olomouc, Czech Republic.

The development of the root apex is determined by progress of cells from the meristematic region to the successive post-mitotic developmental zones for transition, cell elongation and final cell differentiation. We addressed root development, tissue architecture and root developmental zonation by means of light-sheet microscopic imaging of Arabidopsis thaliana seedlings expressing END BINDING protein 1c (EB1c) fused to green fluorescent protein (GFP) under control of native EB1c promoter. Unlike the other two members of the EB1 family, plant-specific EB1c shows prominent nuclear localization in non-dividing cells in all developmental zones of the root apex. Read More

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January 2016

H2-EB1 Molecule Alleviates Allergic Rhinitis Symptoms of H2-Eb1 Knockout Mice.

Iran J Immunol 2015 Dec;12(4):263-73

Department of Otorhinolaryngology, the First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang, China.

Background: H2-EB1 molecule which is the homolog of Human HLA-DRB1 is proposed to be associated with allergic rhinitis (AR). Construction of H2-Eb1 knockout animal models provides a tool to elucidate the role of H2-EB1 and AR pathogenesis.

Objective: To establish the H2-Eb1 knockout model and investigate the H2-EB1 functions in H2-Eb1 knockout mice as a model of AR. Read More

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December 2015