80 results match your criteria degrade pol


Feline Calicivirus Proteinase-Polymerase Protein Degrades mRNAs To Inhibit Host Gene Expression.

J Virol 2021 Jun 10;95(13):e0033621. Epub 2021 Jun 10.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.

To replicate efficiently and evade the antiviral immune response of the host, some viruses degrade host mRNA to induce host gene shutoff via encoding shutoff factors. In this study, we found that feline calicivirus (FCV) infection promotes the degradation of endogenous and exogenous mRNAs and induces host gene shutoff, which results in global inhibition of host protein synthesis. Screening assays revealed that proteinase-polymerase (PP) is a most effective factor in reducing mRNA expression. Read More

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Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products.

Pol J Microbiol 2020 Sep 8;69(3):339-347. Epub 2020 Sep 8.

College of Grassland Science, Shanxi Agricultural University, Taigu, China.

Ligninolytic bacteria degrading lignin were isolates and identified, and their biodegradation mechanism of alkaline-lignin was investigated. Four strains with lignin degradation capability were screened and identified from the soil, straw, and silage based on their decolorizing capacity of aniline blue and colony size on alkaline-lignin medium. The degradation ratio of BY5, LN2, LN4, and CL32 have been assayed using alkaline-lignin as the unique carbon source. Read More

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September 2020

Human cytomegalovirus IE2 drives transcription initiation from a select subset of late infection viral promoters by host RNA polymerase II.

PLoS Pathog 2020 04 6;16(4):e1008402. Epub 2020 Apr 6.

Departments of Internal Medicine and Epidemiology, University of Iowa and Iowa City Veterans Affairs Health Care System, Iowa City, IA, United States of America.

Herpesvirus late promoters activate gene expression after viral DNA synthesis has begun. Alphaherpesviruses utilize a viral immediate-early protein to do this, whereas beta- and gammaherpesviruses primarily use a 6-member set of viral late-acting transcription factors (LTF) that are drawn to a TATT sequence in the late promoter. The betaherpesvirus, human cytomegalovirus (HCMV), produces three immediate-early 2 protein isoforms, IE2-86, IE2-60, IE2-40, late in infection, but whether they activate late viral promoters is unknown. Read More

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Realizing Vibrant and High-Contrast Reflective Structural Colors from Lossy Metals Supporting Dielectric Gratings.

ACS Nano 2019 Sep 3;13(9):10717-10726. Epub 2019 Sep 3.

Department of Chemistry and Nanoscience , Ewha Womans University , Seoul 03760 , Republic of Korea.

Despite their distinctive chemical properties, lossy metals are generally avoided in the design of structural colors because the optical losses can degrade the color vibrancy. Herein, we demonstrate a strategy that allows lossy metals supporting near-wavelength dielectric gratings to achieve high color vibrancy by benefiting from the optical loss rather than suffering from it. By exciting the grating rotated 45° relative to the incident field, s-polarized (s-pol) and p-polarized (p-pol) light each excites a spectrally distinct resonance, described by a treatment of coupled waveguide-array modes, that retards the phase over the wavelength. Read More

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September 2019

Immunohistochemical assessment of metalloproteinases MMP2 and MMP9 expression in canine various subtypes of lymphomas in relation with proliferative and apoptotic markers.

Pol J Vet Sci 2019 Jun;22(2):203-211

Department of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.

Matrix metalloproteinases 2 and 9 (MMP2 and MMP9) are proteolytic enzymes involved with extracellular matrix degradation. They play a role in tumor invasion and metastases. Because of their ability to degrade signaling molecules presented in extracellular matrix, MMPs contribute to tumor proliferation and apoptosis. Read More

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Periplasmic expression of a restriction endonuclease in Escherichia coli and its effect on the antiviral activity of the host.

Acta Biochim Pol 2019 Feb;66(1):83-89

University of Gdansk, Department of Microbiology, Wita Stwosza 59, 80-308 Gdańsk, Poland.

One possible mechanism preventing phage infection of the bacterial cells is related to the presence of an effective restriction-modification system (R-M) which allows restriction of the invading DNA. However, there are some limitations to the absolute restriction of foreign DNA. Since there is a serious conflict between increase in the restriction-modification genes expression level and cell viability, we examined the antiviral effect of EcoRI restriction endonuclease after its translocation to the periplasmic space of the cell. Read More

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February 2019

Isolation of Bacterial Endophytes from and their Potential in Diclofenac and Sulfamethoxazole Degradation.

Pol J Microbiol 2018;67(3):321-331

Environmental Biotechnology Department, Faculty o f Power and Environmental Engineering, Silesian University of Technology, Gliwice, Poland ; Centre for Biotechnology, Silesian University of Technology, Gliwice, Poland.

Diclofenac (DCF), a non-steroidal anti-inflammatory drug (NSAID) and sulfamethoxazole (SMX), an antimicrobial agent, are in common use and can be often detected in the environment. The constructed wetland systems (CWs) are one of the technologies to remove them from the aquatic environment. The final effect of the treatment processes depends on many factors, including the interaction between plants and the plant-associated microorganisms present in the system. Read More

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Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production.

Pol J Microbiol 2018 Jun;67(2):181-190

Institute of Food Technology of Plant Origin, Poznań University of Life Sciences,Poznań,Poland.

A total of 123 Trichoderma strains were isolated from different habitats and tested for their ability to degrade cellulose and xylan by simple plate screening method. Among strains, more than 34 and 45% respectively, exhibited higher cellulolytic and xylanolytic activity, compared to the reference strain T. reesei QM 9414. Read More

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Specific Recognition of Arginine Methylated Histone Tails by JMJD5 and JMJD7.

Sci Rep 2018 02 19;8(1):3275. Epub 2018 Feb 19.

Department of Biomedical Research, National Jewish Health, 1400 Jackson St, Denver, CO, 80206, USA.

We have reported that JMJD5 and JMJD7 (JMJD5/7) are responsible for the clipping of arginine methylated histone tails to generate "tailless nucleosomes", which could release the pausing RNA polymerase II (Pol II) into productive transcription elongation. JMJD5/7 function as endopeptidases that cleave histone tails specifically adjacent to methylated arginine residues and continue to degrade N-terminal residues of histones via their aminopeptidase activity. Here, we report structural and biochemical studies on JMJD5/7 to understand the basis of substrate recognition and catalysis mechanism by this JmjC subfamily. Read More

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February 2018

Bacterial SBP56 identified as a Cu-dependent methanethiol oxidase widely distributed in the biosphere.

ISME J 2018 01 24;12(1):145-160. Epub 2017 Oct 24.

School of Life Sciences, University of Warwick, Coventry, UK.

Oxidation of methanethiol (MT) is a significant step in the sulfur cycle. MT is an intermediate of metabolism of globally significant organosulfur compounds including dimethylsulfoniopropionate (DMSP) and dimethylsulfide (DMS), which have key roles in marine carbon and sulfur cycling. In aerobic bacteria, MT is degraded by a MT oxidase (MTO). Read More

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January 2018

Contribution of increased mutagenesis to the evolution of pollutants-degrading indigenous bacteria.

PLoS One 2017 4;12(8):e0182484. Epub 2017 Aug 4.

Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia.

Bacteria can rapidly evolve mechanisms allowing them to use toxic environmental pollutants as a carbon source. In the current study we examined whether the survival and evolution of indigenous bacteria with the capacity to degrade organic pollutants could be connected with increased mutation frequency. The presence of constitutive and transient mutators was monitored among 53 pollutants-degrading indigenous bacterial strains. Read More

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October 2017

Expression of the insect metalloproteinase inhibitor IMPI in the fat body of Galleria mellonella exposed to infection with Beauveria bassiana.

Acta Biochim Pol 2017 11;64(2):273-278. Epub 2017 Apr 11.

Maria Curie-Sklodowska University, Faculty of Biology and Biotechnology, Institute of Biology and Biochemistry, Department of Immunobiology, Lublin, Poland.

The inducible metalloproteinase inhibitor (IMPI) discovered in Galleria mellonella is currently the only specific inhibitor of metalloproteinases found in animals. Its role is to inhibit the activity of metalloproteinases secreted by pathogenic organisms as virulence factors to degrade immune-relevant polypeptides of the infected host. This is a good example of an evolutionary arms race between the insect hosts and their natural pathogens. Read More

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Matrix metalloproteinases MMP1, MMP2, MMP9 and their tissue inhibitors TIMP1, TIMP2, TIMP3 in head and neck cancer: an immunohistochemical study.

Otolaryngol Pol 2016 Jun;70(3):32-43

Department of Pathology of the Age of Development, Medical University of Lodz.

Introduction: Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes implicated in the invasion and metastasis of many kinds of cancer because of their ability to degrade components of extracellular matrix. Specific tissue inhibitors of matrix metalloproteinases (TIMPs) regulate their activity. MMPs and TIMPs were identified in many neoplasm, including cancers of head and neck, lung, breast and colon cancer. Read More

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Enzymes Involved in Naproxen Degradation by Planococcus sp. S5.

Pol J Microbiol 2016 Jun;65(2):177-182

Department of Biochemistry, Faculty of Biology and Environment Protection, University of Silesia in Katowice, Katowice, Poland.

Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. Planococcus sp. Read More

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Pseudomonas aeruginosa phage PaP1 DNA polymerase is an A-family DNA polymerase demonstrating ssDNA and dsDNA 3'-5' exonuclease activity.

Virus Genes 2016 Aug 6;52(4):538-51. Epub 2016 Apr 6.

Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, People's Republic of China.

Most phages contain DNA polymerases, which are essential for DNA replication and propagation in infected host bacteria. However, our knowledge on phage-encoded DNA polymerases remains limited. This study investigated the function of a novel DNA polymerase of PaP1, which is the lytic phage of Pseudomonas aeruginosa. Read More

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Enzymes Involved in Naproxen Degradation by Planococcus sp. S5.

Pol J Microbiol 2016 ;65(2):177-82

Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. Planococcus sp. Read More

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Elevated Genome-Wide Instability in Yeast Mutants Lacking RNase H Activity.

Genetics 2015 Nov 22;201(3):963-75. Epub 2015 Sep 22.

Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina 27710

Two types of RNA:DNA associations can lead to genome instability: the formation of R-loops during transcription and the incorporation of ribonucleotide monophosphates (rNMPs) into DNA during replication. Both ribonuclease (RNase) H1 and RNase H2 degrade the RNA component of R-loops, whereas only RNase H2 can remove one or a few rNMPs from DNA. We performed high-resolution mapping of mitotic recombination events throughout the yeast genome in diploid strains of Saccharomyces cerevisiae lacking RNase H1 (rnh1Δ), RNase H2 (rnh201Δ), or both RNase H1 and RNase H2 (rnh1Δ rnh201Δ). Read More

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November 2015

The influence of low process temperature on the hydrodynamic radius of polyNIPAM-co-PEG thermosensitive nanoparticles presumed as drug carriers for bioactive proteins.

Acta Pol Pharm 2015 Jan-Feb;72(1):161-9

The aim of the work was to evaluate the influence of low process temperature on the hydrodynamic radius of the synthesized nanoparticles presumed for incorporation of bioactive proteins. The reaction prompted in temperatures of 22, 38 and 70 degrees C. The first one reflected the ambient environmental temperature, at which the bioactive proteins may be implemented into the reactant mixture. Read More

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Laccase production by Trametes hirsuta, characterization, and its capability of decoloring chlorophyll.

Pol J Microbiol 2014 ;63(3):323-33

The present study focused on laccase production, characterization, and its involvement in chlorophyll decolorization. Extracellular laccase, with the highest activity of 11 U/ml on day 8, was efficiently produced from Trametes hirsuta in 5 l bioreactor with optimized media comprising dual carbon sources, glucose and water hyacinth. A laccase was then purified from the supernatant to homogeneity with purification fold of 9. Read More

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[Is there a relationship between pregnancy induced hypertension and obstructive sleep apnea? Case report].

Pneumonol Alergol Pol 2014 ;82(2):156-62

The paper examines the case of a pregnant woman in a twin pregnancy complicated by pre-eclampsia and sleep disordered breathing. The patient was admitted to hospital with high blood pressure, proteinuria and increasing oedema. Laboratory tests revealed proteinuria and reduced total protein concentration in serum. Read More

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December 2015

Effect of microwave heating on the quality characteristics of canola oil in presence of palm olein.

Acta Sci Pol Technol Aliment 2013 Jul-Sep;12(3):241-52

Sime Darby Research Sdn. Bhd. Selangor, Malaysia.

Background: Microwave heating is one of the most attractive cooking methods for food preparation, commonly employed in households and especially in restaurants for its high speed and convenience. The chemical constituents of oils that degrade during microwave heating do so at rates that vary with heating temperature and time in a similar manner to other type of processing methods. The rate of quality characteristics of the oil depends on the fatty acid composition and the minor components during heating. Read More

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October 2014

Cellulase inhibits Burkholderia cepacia biofilms on diverse prosthetic materials.

Pol J Microbiol 2013 ;62(3):327-30

Department of Microbiology, PRIST University, Thanjavur, Tamil Nadu, India.

Burkholderia cepacia is an opportunistic pathogen causing infections in patients with cystic fibrosis. Patients with implanted devices are prone to B. cepacia infections due to its ability to grow as biofilms. Read More

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February 2014

Utilization of 4-n-nonylphenol by Metarhizium sp. isolates.

Acta Biochim Pol 2013 ;60(4):677-82

Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland.

Nonylphenol (4-NP) is a xenobiotic classified as an endocrine disrupting compound with an ability to interfere with hormonal systems of numerous organisms including humans. It is widely distributed not only in aquatic but also in terrestrial systems. The aim of this study was to evaluate the ability of cosmopolitan fungus Metarhizium (commonly persistent in soil as a facultative insect pathogen, controlling populations of arthropods in natural environment) to degrade 4-n-nonylphenol. Read More

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Evaluation of the levels of metalloproteinsase-2 in patients with abdominal aneurysm and abdominal hernias.

Pol Przegl Chir 2013 May;85(5):271-8

Unlabelled: Abdominal aortic aneurysms and abdominal hernias become an important health problems of our times. Abdominal aortic aneurysm and its rupture is one of the most dangerous fact in vascular surgery. There are some theories pointing to a multifactoral genesis of these kinds of diseases, all of them assume the attenuation of abdominal fascia and abdominal aortic wall. Read More

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Is determination of matrix metalloproteinases and their tissue inhibitors serum concentrations useful in patients with gastroenteropancreatic and bronchopulmonary neuroendocrine neoplasms?

Endokrynol Pol 2012 ;63(6):470-6

Division of Endocrinology, Department of Pathophysiology and Endocrinology, Silesian Medical University in Katowice, 40–952 Katowice, Poland.

Introduction: Gastroenteropancreatic (GEP) and bronchopulmonary (BP) neurendocrine neoplasms (NENs) are rare and slowly growing tumours. Matrix metalloproteinases (MMPs) degrade extracellular matrix and are responsible for invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) affect the invasiveness of tumour cells and the formation of distant metastases. Read More

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December 2013

Histone deacetylase activity is required for human oligodendrocyte progenitor differentiation.

Glia 2012 Dec 23;60(12):1944-53. Epub 2012 Aug 23.

Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York 14214, USA.

The molecular mechanisms controlling human oligodendrocyte development are poorly characterized. Microarray analysis of human oligodendrocyte progenitor cells (OPCs) and immature oligodendrocytes revealed that specific-class I histone deacetylase (HDAC) target genes were actively repressed during oligodendrocyte commitment. Although epigenetic regulation of oligodendrocyte differentiation has been established in rodent development, the role of HDACs in human OPCs remains undefined. Read More

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December 2012

Zinc-finger antiviral protein inhibits XMRV infection.

PLoS One 2012 15;7(6):e39159. Epub 2012 Jun 15.

Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Background: The zinc-finger antiviral protein (ZAP) is a host factor that specifically inhibits the replication of certain viruses, including Moloney murine leukemia virus (MoMLV), HIV-1, and certain alphaviruses and filoviruses. ZAP binds to specific viral mRNAs and recruits cellular mRNA degradation machinery to degrade the target RNA. The common features of ZAP-responsive RNA sequences remain elusive and thus whether a virus is susceptible to ZAP can only be determined experimentally. Read More

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December 2012

Regulation of oxidative DNA damage repair: the adenine:8-oxo-guanine problem.

Cell Cycle 2012 Mar 15;11(6):1070-5. Epub 2012 Mar 15.

Institute for Veterinary Biochemistry and Molecular Biology; University of Zürich-Irchel; Zürich, Switzerland.

Reactive oxygen species (ROS) constantly attack DNA. One of the best-characterized oxidative DNA lesions is 7,8-dihydro-8-oxoguanine (8-oxo-G). Many human diseases, such as cancer and neurodegenerative disorders, have been correlated with oxidative DNA damage. Read More

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Isolation and characterization of an endosulfan-degrading strain, Stenotrophomonas sp. LD-6, and its potential in soil bioremediation.

Pol J Microbiol 2012 ;61(4):257-62

Department of Environmental Sciences, College of Environment and Resource Sciences, Zhejiang Agricultural and Forestry University, Linan, China.

Aerobic bacteria degrading endosulfan were isolated from contaminated sludge. One of the isolates, LD-6, was identified as Stenotrophomonas sp. The bacterium could utilize endosulfan as the sole source of carbon and sulfur. Read More

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Regulation of foamy viral transcription and RNA export.

Authors:
Jochen Bodem

Adv Virus Res 2011 ;81:1-31

Institute of Virology and Immunobiology, University of Würzburg, Würzburg, Germany.

Foamy viruses (FVs) are distinct members of the retrovirus (RV) family. In this chapter, the molecular regulation of foamy viral transcription, splicing, polyadenylation, and RNA export will be compared in detail to the orthoretroviruses. Foamy viral transcription is regulated in early and late phases, which are separated by the usage of two promoters. Read More

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