33 results match your criteria define cushion

The Persistent Challenge of Pneumocystis Growth Outside the Mammalian Lung: Past and Future Approaches.

Front Microbiol 2021 20;12:681474. Epub 2021 May 20.

Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, United States.

The pathogenic fungi in the genus, Pneumocystis, have eluded attempts to continuously grow them in an cultivation system. New data from transcriptomic and genomic sequencing studies have identified a myriad of absent metabolic pathways, helping to define their host obligate nature. These nutrients, factors, and co-factors are acquired from their mammalian host and provide clues to further supplementation of existing media formulations. Read More

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Developing a process for assessing equivalency of wheelchair cushion pressure redistribution performance.

Assist Technol 2020 19;32(2):92-99. Epub 2018 Sep 19.

Rehabilitation Engineering and Applied Research Lab, Georgia Institute of Technology, Atlanta, Georgia, USA.

For wheelchair users who are at risk for developing pressure ulcers, cushion prescription seeks to address tissue integrity. Because many designs of wheelchair cushions exist, a need exists to estimate their performance in supporting the body. The objective of this research was to develop an approach to assess the equivalency of cushions with respect to their pressure redistribution performance. Read More

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January 2021

Defining the hierarchical organisation of collagen VI microfibrils at nanometre to micrometre length scales.

Acta Biomater 2017 04 10;52:21-32. Epub 2016 Dec 10.

Wellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, M13 9PT, UK. Electronic address:

Extracellular matrix microfibrils are critical components of connective tissues with a wide range of mechanical and cellular signalling functions. Collagen VI is a heteromeric network-forming collagen which is expressed in tissues such as skin, lung, blood vessels and articular cartilage where it anchors cells into the matrix allowing for transduction of biochemical and mechanical signals. It is not understood how collagen VI is arranged into microfibrils or how these microfibrils are arranged into tissues. Read More

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MRI quantification of pancreas motion as a function of patient setup for particle therapy -a preliminary study.

J Appl Clin Med Phys 2016 09 8;17(5):60-75. Epub 2016 Sep 8.

Centro Nazionale di Adroterapia Oncologica.

Particle therapy (PT) has shown positive therapeutic results in local control of locally advanced pancreatic lesions. PT effectiveness is highly influenced by target localization accuracy both in space, since the pancreas is located in proximity to radiosensitive vital organs, and in time as it is subject to substantial breathing-related motion. The purpose of this preliminary study was to quantify pancreas range of motion under typical PT treatment conditions. Read More

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September 2016

Wnt/β-catenin signaling enables developmental transitions during valvulogenesis.

Development 2016 Mar 18;143(6):1041-54. Epub 2016 Feb 18.

Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA Department of Biology, University of Oregon, Eugene, OR 97403-1229, USA

Heart valve development proceeds through coordinated steps by which endocardial cushions (ECs) form thin, elongated and stratified valves. Wnt signaling and its canonical effector β-catenin are proposed to contribute to endocardial-to-mesenchymal transformation (EMT) through postnatal steps of valvulogenesis. However, genetic redundancy and lethality have made it challenging to define specific roles of the canonical Wnt pathway at different stages of valve formation. Read More

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Comparison of analytical sensitivity and women's interpretation of home pregnancy tests.

Clin Chem Lab Med 2015 Feb;53(3):391-402

Background: Internationally there are no defined standards for expressing the performance of home-based pregnancy tests, nor any pre-defined accuracy standard requirements. The aim of this study was to evaluate the accuracy of a selection of home-based pregnancy tests relative to their packaging/product insert claims.

Methods: Eight home-based pregnancy tests were evaluated using human chorionic gonadotrophin (hCG) urine standards (0, 15, 25 mlU/mL). Read More

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February 2015

A nanometric cushion for enhancing scratch and wear resistance of hard films.

Beilstein J Nanotechnol 2014 10;5:1005-15. Epub 2014 Jul 10.

Department of Chemical Research Support, Weizmann Institute of Science, Rehovot 76100, Israel.

Scratch resistance and friction are core properties which define the tribological characteristics of materials. Attempts to optimize these quantities at solid surfaces are the subject of intense technological interest. The capability to modulate these surface properties while preserving both the bulk properties of the materials and a well-defined, constant chemical composition of the surface is particularly attractive. Read More

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Imbalanced expression of Vcan mRNA splice form proteins alters heart morphology and cellular protein profiles.

PLoS One 2014 20;9(2):e89133. Epub 2014 Feb 20.

Departments of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, South Carolina, United States of America.

The fundamental importance of the proteoglycan versican to early heart formation was clearly demonstrated by the Vcan null mouse called heart defect (hdf). Total absence of the Vcan gene halts heart development at a stage prior to the heart's pulmonary/aortic outlet segment growth. This creates a problem for determining the significance of versican's expression in the forming valve precursors and vascular wall of the pulmonary and aortic roots. Read More

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January 2015

Burst fractures of the lumbar spine in frontal crashes.

Accid Anal Prev 2013 Oct 3;59:153-63. Epub 2013 Jun 3.

Harborview Injury Prevention and Research Center, University of Washington, P.O. Box 359960, 325 Ninth Avenue, Seattle, WA 98104, United States. Electronic address:

Background: In the United States, major compression and burst type fractures (>20% height loss) of the lumbar spine occur as a result of motor vehicle crashes, despite the improvements in restraint technologies. Lumbar burst fractures typically require an axial compressive load and have been known to occur during a non-horizontal crash event that involve high vertical components of loading. Recently these fracture patterns have also been observed in pure horizontal frontal crashes. Read More

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October 2013

Apelin-APJ signaling is a critical regulator of endothelial MEF2 activation in cardiovascular development.

Circ Res 2013 Jun 19;113(1):22-31. Epub 2013 Apr 19.

Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06511, USA.

Rationale: The peptide ligand apelin and its receptor APJ constitute a signaling pathway with numerous effects on the cardiovascular system, including cardiovascular development in model organisms such as xenopus and zebrafish.

Objective: This study aimed to characterize the embryonic lethal phenotype of the Apj-/- mice and to define the involved downstream signaling targets.

Methods And Results: We report the first characterization of the embryonic lethality of the Apj-/- mice. Read More

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A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.

Kidney Int 2012 Jun 14;81(12):1263-72. Epub 2012 Mar 14.

Laboratory of Mass Spectrometry and Proteomics, Institute of Protein Biochemistry - CNR, Naples, Italy.

Urinary exosomes have received considerable attention as a potential biomarker source for the diagnosis of renal diseases. Notwithstanding, their use in protein biomarker research is hampered by the lack of efficient methods for vesicle isolation, lysis, and protein quantification. Here we report an improved ultracentrifugation-based method that facilitates the solubilization and removal of major impurities associated with urinary exosomes. Read More

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ETS-dependent regulation of a distal Gata4 cardiac enhancer.

Dev Biol 2012 Jan 26;361(2):439-49. Epub 2011 Oct 26.

Cardiovascular Research Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158-2517, USA.

The developing heart contains an inner tube of specialized endothelium known as endocardium, which performs multiple essential functions. In spite of the essential role of the endocardium in heart development and function, the transcriptional pathways that regulate its development remain largely undefined. GATA4 is a zinc finger transcription factor that is expressed in multiple cardiovascular lineages and is required for endocardial cushion development and embryonic viability, but the transcriptional pathways upstream of Gata4 in the endocardium and its derivatives in the endocardial cushions are unknown. Read More

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January 2012

Design and milling manufacture of polyurethane custom contoured cushions for wheelchair users.

Australas Med J 2011 30;4(9):500-6. Epub 2011 Sep 30.

Universidade Federal do Rio Grande do Sul, Brazil.

Background: The design of custom contoured cushions manufactured in flexible polyurethane foams is an option to improve positioning and comfort for people with disabilities that spend most of the day seated in the same position. These surfaces increase the contact area between the seat and the user. This fact contributes to minimise the local pressures that can generate problems like decubitus ulcers. Read More

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February 2013

Optimal trocar placement for ergonomic intracorporeal sewing and knotting in laparoscopic hiatal surgery.

Am J Surg 2010 Oct 16;200(4):519-28. Epub 2010 Jul 16.

First Department of Surgery, Hippocration Hospital, University of Athens Medical School, Athens, Greece.

Background: Trocar placement presently is mostly empiric. Our goal was to define simple distances from bony landmarks to locate the optimal ergonomic placement of manipulation trocars for access to the lower esophagus and hiatal orifice, for suture placement, and knotting of the gastric fundus and crura. Hypothesizing that the ideal ergonomic principles of a manipulation angle of 60°, an elevation angle (α(e)) of 30° to 60°, and an intracorporeal/extracorporeal length ratio (I/E) of working instruments close to 1:1 are interrelated by simple trigonometric functions, the variations of each of these parameters were calculated in a dependent manner for 2 standard lengths of needle holders: 48. Read More

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October 2010

Shared gene expression profiles in developing heart valves and osteoblast progenitor cells.

Physiol Genomics 2008 Sep 8;35(1):75-85. Epub 2008 Jul 8.

Division of Molecular Cardiovascular Biology, Cincinnati Children's Medical Center, Cincinnati, Ohio 45229, USA.

The atrioventricular (AV) valves of the heart develop from undifferentiated mesenchymal endocardial cushions, which later mature into stratified valves with diversified extracellular matrix (ECM). Because the mature valves express genes associated with osteogenesis and exhibit disease-associated calcification, we hypothesized the existence of shared regulatory pathways active in developing AV valves and in bone progenitor cells. To define gene regulatory programs of valvulogenesis relative to osteoblast progenitors, we undertook Affymetrix gene expression profiling analysis of murine embryonic day (E)12. Read More

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September 2008

Carbon dioxide rebreathing during non-invasive ventilation delivered by helmet: a bench study.

Intensive Care Med 2008 Aug 18;34(8):1454-60. Epub 2008 Apr 18.

Servizio di Anestesia e Rianimazione I, Fondazione IRCCS Policlinico S. Matteo, Piazzale Golgi 2, Pavia, Italy.

Objective: To define how to monitor and limit CO(2) rebreathing during helmet ventilation.

Design: Physical model study.

Setting: Laboratory in a university teaching hospital. Read More

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Independent requirements for Hedgehog signaling by both the anterior heart field and neural crest cells for outflow tract development.

Development 2007 Apr 7;134(8):1593-604. Epub 2007 Mar 7.

Departments of Cell Biology and Pediatrics, Neonatal-Perinatal Research Institute, Duke University Medical Center, Durham, NC 27710, USA.

Cardiac outflow tract (OFT) septation is crucial to the formation of the aortic and pulmonary arteries. Defects in the formation of the OFT can result in serious congenital heart defects. Two cell populations, the anterior heart field (AHF) and cardiac neural crest cells (CNCCs), are crucial for OFT development and septation. Read More

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A 4.3 kb Smad7 promoter is able to specify gene expression during mouse development.

Biochim Biophys Acta 2007 Feb 19;1769(2):149-52. Epub 2007 Jan 19.

Department of Medical and Molecular Genetics and the Walther Oncology Center, Indiana University School of Medicine, 975 West Walnut Street, Indianapolis, IN 46202, USA.

Members of transforming growth factor-beta (TGF-beta) superfamily play important roles in diverse biological functions including early development. These extracellular factors exert their effects by interacting with membrane receptors followed by signal transduction by a group of Smad proteins. Smad7 is an inhibitory Smad protein that specifically antagonizes TGF-beta and activin signaling. Read More

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February 2007

Bone morphogenetic protein receptor 1A signaling is dispensable for hematopoietic development but essential for vessel and atrioventricular endocardial cushion formation.

Development 2006 Sep 3;133(17):3473-84. Epub 2006 Aug 3.

Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid, St Louis, MO 63110, USA.

Bone morphogenetic protein 4 (BMP4) is crucial for the formation of FLK1-expressing (FLK1(+)) mesodermal cells. To further define the requirement for BMP signaling in the differentiation of blood, endothelial and smooth muscle cells from FLK1(+) mesoderm, we inactivated Alk3 (Bmpr1a) in FLK1(+) cells by crossing Alk3(floxed/floxed) and Flk1(+/Cre)Alk3(+/floxed) mice. Alk3 conditional knockout (CKO) mice died between E10. Read More

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September 2006

The Balanced Budget Act of 1997 and the financial health of teaching hospitals.

Ann Fam Med 2004 Jan-Feb;2(1):71-8

The Robert Graham Center, Policy Studies in Family Practice and Primary Care, Washington, DC 20036, USA.

Background: We wanted to evaluate the most recent, complete data related to the specific effects of the Balanced Budget Act of 1997 relative to the overall financial health of teaching hospitals. We also define cost report variables and calculations necessary for continued impact monitoring.

Methods: We undertook a descriptive analysis of hospital cost report variables for 1996, 1998, and 1999, using simple calculations of total, Medicare, prospective payment system, graduate medical education (GME), and bad debt margins, as well as the proportion with negative total operating margins. Read More

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Protein-protein interactions: structurally conserved residues distinguish between binding sites and exposed protein surfaces.

Proc Natl Acad Sci U S A 2003 May 1;100(10):5772-7. Epub 2003 May 1.

Basic Research Program, SAIC-Frederick, Inc., Laboratory of Experimental and Computational Biology, National Cancer Institute, Frederick, MD 21702, USA.

Polar residue hot spots have been observed at protein-protein binding sites. Here we show that hot spots occur predominantly at the interfaces of macromolecular complexes, distinguishing binding sites from the remainder of the surface. Consequently, hot spots can be used to define binding epitopes. Read More

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Temporal and distinct TGFbeta ligand requirements during mouse and avian endocardial cushion morphogenesis.

Dev Biol 2002 Aug;248(1):170-81

Department of Biochemistry and Molecular Biology, Mayo Clinic, Scottsdale, AZ 85259, USA.

The formation of endocardial cushions in the atrioventricular (AV) canal of the rudimentary heart requires epithelial-to-mesenchymal cell transformation (EMT). This is a complex developmental process regulated by multiple extracellular signals and transduction pathways. A collagen gel assay, long used to examine endocardial cushion development in avian models, is now being employed to investigate genetically engineered mouse models with abnormal heart morphogenesis. Read More

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Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram syndrome.

Dev Biol 1999 Jul;211(1):100-8

Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts, 02115, USA.

To further define the role of a T-box transcription factor, Tbx5, in cardiac development, we have examined its expression in the developing mouse and chick heart and correlated this pattern with cardiac defects caused by human TBX5 mutations in Holt-Oram syndrome. Early in the developing heart, Tbx5 is uniformly expressed throughout the entire cardiac crescent. Upon formation of the linear heart tube, Tbx5 is expressed in a graded fashion, stronger near the posterior end and weaker at the anterior end. Read More

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An autocrine function for transforming growth factor (TGF)-beta3 in the transformation of atrioventricular canal endocardium into mesenchyme during chick heart development.

Dev Biol 1998 Feb;194(1):99-113

Department of Anatomy, Saitama Medical School, Saitama, 350-0495, Japan.

Transformation of atrioventricular canal endocardium into invasive mesenchyme is a critical antecedent of cardiac septation and valvulogenesis. Previous studies by Potts et al. (Proc. Read More

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February 1998

Cardiotrophin-1 displays early expression in the murine heart tube and promotes cardiac myocyte survival.

Development 1996 Feb;122(2):419-28

Department of Medicine, Center for Molecular Genetics, University of California , San Diego, School of Medicine, La Jolla, 92093-0613, USA.

We have recently isolated a novel cytokine, cardiotrophin-1 (CT-1), from an in vitro embryonic stem cell system of cardiogenesis that can activate embryonic markers in neonatal rat cardiac myocytes. CT-1 is a new member of the interleukin 6 (IL-6)/leukemia inhibitory factor (LIF) cytokines, which activate downstream signals via gp130-dependent pathways. To define the developmental pattern of expression of CT-1 during murine embryogenesis, we have developed antibodies directed against a CT-1 fusion protein. Read More

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February 1996

Establishing the anatomic hallmarks of congenitally malformed hearts.

Trends Cardiovasc Med 1996 Jan;6(1):10-5

Robert H. Anderson, Sandra Webb, and Nigel A. Brown are at the Department of Pediatrics, National Heart & Lung Institute, Royal Brompton Hospital, London SW3 6LY, United Kingdom.; the MRC Experimental Embryology, St. George's Hospital Medical School, London, United Kingdom.; Teratology Unit, St. George's Hospital Medical School, London, United Kingdom.

A detailed knowledge of the precise morphology of the congenitally malformed heart has never been more important. In fields as diverse as clinical genetic counseling, epidemiology, surgery, and development biology, a proper description of morphology is essential to understand the cause and prevention of cardiac malformations. There has been a tendency in the past to categorize malformed hearts on the basis of putative developmental faults that are deduced from abnormal morphology, for example "endocardial cushion" or "looping" defects. Read More

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January 1996

Chylothorax associated with right-sided heart failure in five cats.

J Am Vet Med Assoc 1994 Jan;204(1):84-9

Department of Small Animal Medicine and Surgery, Texas Veterinary Medical Center, College of Veterinary Medicine, Texas A&M University, College Station 77843.

Chylothorax associated with right-sided congestive heart failure was diagnosed in 5 cats. One cat had restrictive pericardial disease, with mild pericardial effusion, and a heart-base chemodectoma. Two other cats had congestive cardiac disease (tetralogy of Fallot and tricuspid regurgitation in 1 cat, and endocardial cushion defect and tricuspid dysplasia in the other), and 2 cats had idiopathic cardiomyopathy. Read More

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January 1994

Factors affecting seat contour characteristics.

J Rehabil Res Dev 1990 ;27(2):127-34

University of Virginia, Rehabilitation Engineering Center, Charlottesville 22903.

This project focused on identifying the influence of subject characteristics and foam properties on seat contours in order to explain the load transfer between the buttocks and cushion. Seat contours were recorded for 17 people (11 spinal cord injured and six able-bodied individuals). Contour characteristics were represented by maximum contour depth, surface area, and displaced volume. Read More

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Release of histamine from human leukocytes by one preparation of IgG oligomers.

Allergy 1985 Apr;40(3):151-6

In preliminary experiments aimed at investigating the effect of covalently cross-linked human myeloma subclass proteins on histamine release from human leukocytes, we observed one preparation (designated here IgG-HR) made from pooled, purified immunoglobulin G, which consistently released histamine from these cells. Dimers and trimers, but not monomers isolated from columns of Sephadex G-200 and Ultrogel AcA22 following incubation of immunoglobulin G (Nordic Laboratories) with dimethyl suberimidate, released histamine from cells of all donors tested. In contrast, cells from the same donors showed variable responsiveness to dimers of IgE (prepared by similar techniques) or to anti-IgE. Read More

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[Cardiopathies in trisomy 21. Therapeutic indications].

Arch Mal Coeur Vaiss 1981 Dec;74(12):1427-36

Approximately 50% of patients with Trisomy 21 have congenital heart lesions and they account for 2.7% of the admissions to the Pediatric Cardiac Unit of the Cardiac Hospital of Lyons. In a series of 91 cases observed over a 10 year period (1070-1980) undergoing hemodynamic and angiographic investigation, 9 out of 10 cases - as previously reported - had the following abnormalities: endocardial cushion defect, 42. Read More

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December 1981