9,650 results match your criteria decreased oxidized


Nrf2 deficiency decreases NADPH from impaired IDH shuttle and pentose phosphate pathway in retinal pigmented epithelial cells to magnify oxidative stress-induced mitochondrial dysfunction.

Aging Cell 2021 Jul 27:e13444. Epub 2021 Jul 27.

Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD, USA.

The nuclear factor-erythroid 2-related factor-2 (Nrf2), a major antioxidant transcription factor, is decreased in several age-related diseases including age-related macular degeneration (AMD), the most common cause of blindness among the elderly in western society. Since Nrf2's mito-protective response is understudied, we investigated its antioxidant response on mitochondria. Control and Nrf2-deficient retinal pigmented epithelial (RPE) cells were compared after treating with cigarette smoke extract (CSE). Read More

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Structural and Functional Impairments of Reconstituted High-Density Lipoprotein by Incorporation of Recombinant β-Amyloid42.

Authors:
Kyung-Hyun Cho

Molecules 2021 Jul 16;26(14). Epub 2021 Jul 16.

Korea Research Institute of Lipoproteins, Medical Innovation Complex, Daegu 41061, Korea.

Beta (β)-amyloid (Aβ) is a causative protein of Alzheimer's disease (AD). In the pathogenesis of AD, the apolipoprotein (apo) A-I and high-density lipoprotein (HDL) metabolism is essential for the clearance of Aβ. In this study, recombinant Aβ42 was expressed and purified via the pET-30a expression vector and production system to elucidate the physiological effects of Aβ on HDL metabolism. Read More

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Bone mineral density and oxidative stress in adolescent girls with anorexia nervosa.

Eur J Pediatr 2021 Jul 22. Epub 2021 Jul 22.

Department of Pediatrics, Faculty of Medicine, National Institute of Children's Diseases, Comenius University, Limbová 1, 833 40, Bratislava, Slovakia.

Oxidative stress appears to be involved in the pathogenesis of osteoporosis-a serious complication of anorexia nervosa (AN). We evaluated the oxidative status in adolescent girls with AN and its potential relationship with bone mineral density (BMD). Girls with AN (n = 43) and age-matched healthy controls (n = 20) underwent anthropometric and BMD examination. Read More

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Maternal Exposure to Oxidized Soybean Oil Impairs Placental Development by Modulating Nutrient Transporters In A Rat Model.

Mol Nutr Food Res 2021 Jul 21:e2100301. Epub 2021 Jul 21.

Institute of Animal Nutrition, Northeast Agricultural University, Harbin, 150030, People's Republic of China.

Scope: As an exogenous food contaminant, dietary oxidized lipid impairs growth and development, trigger chronic diseases in humans or animals. This study explores the effects of soybean oil with different oxidative degree on the placental injury of gestational rats.

Method And Results: Thirty-two female adult rats were randomly assigned to 4 groups. Read More

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G protein-coupled receptor 39 activation alleviates oxidized low-density lipoprotein-induced macrophage inflammatory response, lipid accumulation and apoptosis by inducing A20 expression.

Bioengineered 2021 Dec;12(1):4070-4080

Department of Vascular Surgery, The First Affiliated Hospital of USTC, Hefei City, Anhui Province, China.

G protein-coupled receptor 39 (GPR39) agonist weakens oxidized low-density lipoprotein (ox-LDL)-induced attachment of monocytes to vascular endothelial cells and thus alleviates atherosclerosis. This study looks at whether GPR39 protects macrophages against ox-LDL-induced inflammation and apoptosis and ameliorates lipid accumulation in atherosclerosis and investigates its mechanism. Following inducement of ox-LDL, the expression of GPR39 and tumor necrosis factor alpha-induced protein 3 (TNFAIP3, also known as A20) in Raw 264. Read More

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December 2021

Redox index of Cys-thiol residues of serum apolipoprotein E and its diagnostic potential.

Biosci Rep 2021 Jul 21. Epub 2021 Jul 21.

University of Tsukuba, Tsukuba, Japan.

Background: The redox modulation of Cys-thiol participates in various pathophysiological processes. We explored the proper index for estimating the redox status of Cys-thiol of serum apolipoprotein E (apoE), named "redox-IDX-apoE," which is necessary to understand the redox biology of age-related diseases.

Methods: The fractions of the reduced form (red-), reversible oxidized form (roxi-), and irreversibly oxidized form (oxi-) apoE in serum, obtained from the patients with no apparent disease (controls, n=192) and with atherosclerosis and type 2 diabetes (patients, n=16), were measured by a band-shift assay using a maleimide compound. Read More

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Effect of thioltransferase on oxidative stress induced by high glucose and advanced glycation end products in human lens epithelial cells.

Int J Ophthalmol 2021 18;14(7):965-972. Epub 2021 Jul 18.

Xi'an People's Hospital (Xi'an Fourth Hospital), Shaanxi Eye Hospital, Affiliated Guangren Hospital School of Medicine, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.

Aim: To study the effect of thioltransferase (TTase) on oxidative stress in human lens epithelial cells (HLECs) induced by high glucose and advanced glycation end products (AGEs).

Methods: HLECs were treated with 35.5 mmol/L glucose or 1. Read More

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Data-Independent Acquisition Proteomics Reveals Long-Term Biomarkers in the Serum of C57BL/6J Mice Following Local High-Dose Heart Irradiation.

Front Public Health 2021 2;9:678856. Epub 2021 Jul 2.

Institute of Radiation Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.

Cardiotoxicity is a well-known adverse effect of radiation therapy. Measurable abnormalities in the heart function indicate advanced and often irreversible heart damage. Therefore, early detection of cardiac toxicity is necessary to delay and alleviate the development of the disease. Read More

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Naringin Chelates Excessive Iron and Prevents the Formation of Amyloid-Beta Plaques in the Hippocampus of Iron-Overloaded Mice.

Front Pharmacol 2021 2;12:651156. Epub 2021 Jul 2.

Neuroscience Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

Metal chelating agents are antioxidant agents, which decrease the reductive potential and stabilize the oxidized metal ion form. In this study, we evaluated the naringin capacity in chelating iron and preventing amyloid-beta plaque formation in the hippocampus of iron-overloaded mice. Thirty-five NMRI male mice (8-10 weeks old) were provided. Read More

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Circ-USP9X inhibition reduces ox-LDL-induced endothelial cell injury via the miR-599/CLIC4 axis.

J Cardiovasc Pharmacol 2021 Jul 9. Epub 2021 Jul 9.

Department of Vascular Intervention, The Ninth Hospital of Xi'an, Xi'an City, Shanxi Province, China Department of Oncology, Xi' an Hospital of Traditional Chinese Medicine, Xi'an City, Shanxi Province, China.

Abstract: Atherosclerosis (AS) is the common pathological basis of cardiovascular disease. Circular RNA circ-USP9X (hsa_circ_0090231) has been discovered to be upregulated in oxidized low-density lipoprotein (ox-LDL)-induced HUVEC (Human Umbilical Vein Endothelial Cells), but the role of circ-USP9X in ox-LDL-induced endothelial cell injury is indistinct. The purpose of the research was to investigate the role and regulatory mechanism of circ-USP9X in ox-LDL-induced endothelial cell injury. Read More

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Construction and characterization of novel bifunctional fusion proteins composed of alcohol dehydrogenase and NADH oxidase with efficient oxidized cofactor regeneration.

Biotechnol Appl Biochem 2021 Jul 16. Epub 2021 Jul 16.

Sub-Institute of Agriculture and Food Standardization, China National Institute of Standardization, Beijing, 100191, China.

To tune the efficiency of oxidized cofactor recycling between alcohol dehydrogenase (ADH) and NADH oxidase (NOX) for the production of aromatic chiral alcohols, we designed and constructed four novel bifunctional fusion proteins composed of thermostable ADH and NOX from Thermococcus kodakarensis KOD1. ADH was linked to the N- or C-terminus of NOX with a typical rigid linker (EAAAK) and a flexible linker (GGGGS) , respectively. Compared with the parental enzymes, the NOX moieties in the four fusion proteins exhibited higher specific activities (141-282%), while the ADH moieties exhibited varying levels of specific activity (69-167%). Read More

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Enhancing degradation of atrazine by Fe-phenol modified biochar/ferrate(VI) under alkaline conditions: Analysis of the mechanism and intermediate products.

Chemosphere 2021 Jul 4;285:131399. Epub 2021 Jul 4.

School of Environmental and Safety Engineering, Changzhou University, Changzhou, 213164, China; Changzhou University Huaide College, Jingjiang, 214500, China. Electronic address:

In this study, Fe-phenol modified biochar was prepared to enhance atrazine (AT) degradation by ferrate (Fe(VI)) under alkaline conditions, and the properties, mechanism and transformation pathways were extensively investigated. Degradation experiments showed that Fe-phenol modified biochar was more beneficial for improving the oxidation capacity of Fe(VI) than unmodified biochar, and the biochar with a molar ratio of Fe to phenol of 0.1:5 (BC-2) showed the best promoting effect, and more than 94% of AT was removed at pH = 8 within 30 min. Read More

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Comprehensive exploration of heavy metal contamination and risk assessment at two common smelter sites.

Chemosphere 2021 Jun 29;285:131350. Epub 2021 Jun 29.

Key Laboratory of Pollution Ecology and Environment Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China. Electronic address:

This study investigated the horizontal, vertical and fractional distribution of heavy metals in the soil and the pollution and risk assessment of two smelter sites in Daye (a Cu smelter) and Zhuzhou (a Zn oxide smelter). Nine sampling points were reasonably established at each site, and nine soil samples were collected in each soil profile, with a total of 81 samples at each site. The results indicated that only As concentration was exceeded in most of the samples from the Daye site, and several were contaminated with multiple heavy metals, i. Read More

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Effects of resveratrol on lipid and protein co-oxidation in fish oil-enriched whey protein isolate emulsions.

Food Chem 2021 Jul 5;365:130525. Epub 2021 Jul 5.

Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, Zhejiang, People's Republic of China. Electronic address:

In this study, the impact of resveratrol (RES) on co-oxidation of lipid and protein in a fish oil-fortified whey protein isolate (WPI) emulsion was investigated. Oil-in-water (O/W) emulsions containing 1% fish oil, 6 mg/mL of WPI and RES (0.08 ~ 2 mM) were oxidatively stressed using a Fenton system at 25 °C for 24 h. Read More

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The effect of vitamin D supplementation on flow-mediated dilatation, oxidized LDL and intracellular adhesion molecule 1 on type 2 diabetic patients with hypertension: A randomized, placebo-controlled, double-blind trial.

Diabetes Metab Syndr 2021 Jul 5;15(4):102200. Epub 2021 Jul 5.

Department of Biostatistics, Faculty of Health, Ilam University of Medical Sciences, Ilam, Iran.

Aims: Current study aimed to evaluate the effect of vitamin D supplementation on flow-mediated dilatation (FMD), oxidized LDL (oxLDL) and intracellular adhesion molecule 1 (ICAM1) in type 2 diabetic patients with hypertension.

Methods: In a double-blinded, placebo-controlled trial, 44 patients were randomly divided into vitamin D group (2000 IU/d, n = 23) and placebo group (control, n = 21) for 12 weeks. Vascular function with FMD, Serum 25-OH vitamin D, oxLDL and ICAM1 were assessed at the baseline and after the intervention. Read More

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Cadmium uncouples mitochondrial oxidative phosphorylation and induces oxidative cellular stress in soybean roots.

Environ Sci Pollut Res Int 2021 Jul 14. Epub 2021 Jul 14.

Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, Maringá, PR, 87020-900, Brazil.

Cadmium (Cd) inhibits soybean root growth, but its exact mode of action is still not completely understood. We evaluated the effects of Cd on growth, mitochondrial respiration, lipid peroxidation, total phenols, glutathione, and activities of lipoxygenase (LOX), superoxide dismutase (SOD), and catalase (CAT) in soybean roots. In primary roots, Cd stimulated KCN-insensitive respiration and KCN-SHAM-insensitive respiration, indicating the involvement of the alternative oxidase (AOX) pathway, while it decreased KCN-sensitive respiration, suggesting an inhibition of the cytochrome oxidase pathway (COX). Read More

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Pea protein isolate characteristics modulate functional properties of pea protein-cranberry polyphenol particles.

Food Sci Nutr 2021 Jul 24;9(7):3740-3751. Epub 2021 May 24.

Plants for Human Health Institute North Carolina State University Kannapolis NC USA.

Plant polyphenols have a natural binding affinity for proteins, and their interaction can be exploited to form diverse aggregate particles. Protein-polyphenol particles utilized as food ingredients allow consumers to incorporate more health-benefiting plant bioactives into their diets. The functional properties of the protein-polyphenol particles can be influenced by many factors, including complexation conditions and starting material properties. Read More

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Human d-lactate dehydrogenase deficiency by mutation in a patient with neurological manifestations and mitochondrial complex IV deficiency.

JIMD Rep 2021 Jul 21;60(1):15-22. Epub 2021 May 21.

Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine The University of Hong Kong Hong Kong SAR China.

Background: d-lactate, one of the isomers of lactate, exists in a low concentration in healthy individuals and it can be oxidized to pyruvate catalyzed by d-lactate dehydrogenase. Excessive amount of d-lactate causes d-lactate acidosis associated with neurological manifestations.

Methods And Results: We report here a patient with developmental delay, cerebellar ataxia, and transient hepatomegaly. Read More

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Direct detection of diphenylamino radical formed by oxidation of diphenylamine using APCI-MS.

Rapid Commun Mass Spectrom 2021 Jul 13:e9163. Epub 2021 Jul 13.

Department of Chemistry, Sejong University, Seoul, Republic of Korea.

Rationale: Diphenylamine (DPAH) and its derivatives have been widely used as antioxidants. Its antioxidation behaviors are started to form diphenylamino radical (DPA radical, DPA ) by loss of hydrogen atom through reaction with oxygen or ozone. DPAH was oxidized by ozonation to produce DPA and the DPA was directly detected using APCI-MS. Read More

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miR-100-5p in human umbilical cord mesenchymal stem cell-derived exosomes mediates eosinophilic inflammation to alleviate atherosclerosis via the FZD5/Wnt/β-catenin pathway.

Acta Biochim Biophys Sin (Shanghai) 2021 Jul 13. Epub 2021 Jul 13.

Department of Cardiovascular Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

Exosomes derived from human umbilical cord mesenchymal stem cells (hUMSC-Ex) play important roles in immune and inflammation diseases. However, the role of hUMSC-Ex in atherosclerosis has not been elucidated. In this study, the isolated exosomes were identified by transmission electron microscopy and nanoparticle tracking analysis. Read More

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Propofol Ameliorates ox-LDL-Induced Endothelial Damage Through Enhancing Autophagy via PI3K/Akt/m-TOR Pathway: A Novel Therapeutic Strategy in Atherosclerosis.

Front Mol Biosci 2021 25;8:695336. Epub 2021 Jun 25.

The First School of Clinical Medicine, Lanzhou University, Lanzhou, China.

Atherosclerosis (AS) represents a common age-associated disease, which may be accelerated by oxidized low-density lipoprotein (ox-LDL)-induced endothelial cell injury. This study aimed to investigate the effects of Propofol on ox-LDL-induced endothelial damage and the underlying molecular mechanisms. Human umbilical vein endothelial cells (HUVECs) were exposed to ox-LDL to induce endothelial damage. Read More

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Caffeine prevents oxalate-induced epithelial-mesenchymal transition of renal tubular cells by its anti-oxidative property through activation of Nrf2 signaling and suppression of Snail1 transcription factor.

Biomed Pharmacother 2021 Jul 7;141:111870. Epub 2021 Jul 7.

Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Electronic address:

Caffeine is an active ingredient found in coffee and energy beverages. Its hepatoprotective effects against liver fibrosis are well-documented. Nonetheless, its renoprotective effects against renal fibrogenesis and epithelial-mesenchymal transition (EMT) processes remain unclear and under-investigated. Read More

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Interactions between the antibiotic tetracycline and humic acid: Examination of the binding sites, and effects of complexation on the oxidation of tetracycline.

Water Res 2021 Jun 22;202:117379. Epub 2021 Jun 22.

MOE Key Laboratory of Deep Earth Science and Engineering, College of Architecture and Environment, Sichuan University, Chengdu 610065, China. Electronic address:

The binding between dissolved organic matter (DOM) and micro-pollutants (MPs) results in significant impacts on their migration, transformation and degradation. However, the role of the DOM/MP binding on their oxidative transformation remains poorly studied. The binding of MPs by DOM, in combination with DOM's roles as a photosensitizer and/or a competitor for free radicals, needs to be considered in the context of understanding the DOM's impacts on the oxidative degradation of MPs. Read More

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A high-fat diet exacerbates the Alzheimer's disease pathology in the hippocampus of the App knock-in mouse model.

Aging Cell 2021 Jul 10:e13429. Epub 2021 Jul 10.

Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Insulin resistance and diabetes mellitus are major risk factors for Alzheimer's disease (AD), and studies with transgenic mouse models of AD have provided supportive evidence with some controversies. To overcome potential artifacts derived from transgenes, we used a knock-in mouse model, App , which accumulates Aβ plaques from 6 months of age and shows mild cognitive impairment at 18 months of age, without the overproduction of APP. In the present study, 6-month-old male App and wild-type mice were fed a regular or high-fat diet (HFD) for 12 months. Read More

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Bmal1 Regulates the Redox Rhythm of HSPB1, and Homooxidized HSPB1 Attenuates the Oxidative Stress Injury of Cardiomyocytes.

Oxid Med Cell Longev 2021 18;2021:5542815. Epub 2021 Jun 18.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Institute of Emergency Medicine, The First Affiliated Hospital of Hunan Normal University (Hunan Provincial People's Hospital), Changsha, Hunan, China.

Oxidative stress is the main cause of acute myocardial infarction (AMI), which is related to the disorder of the regulation of Bmal1 on the redox state. HSPB1 form homologous-oxidized HSPB1 (homooxidized HSPB1) to resist oxidative damage via S-thiolated modification. However, it is still unclarified whether there is an interaction between the circadian clock and HSPB1 in myocardial injury. Read More

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CircMTO1 inhibits ox-LDL-stimulated vascular smooth muscle cell proliferation and migration via regulating the miR-182-5p/RASA1 axis.

Mol Med 2021 Jul 8;27(1):73. Epub 2021 Jul 8.

Department of Cardiology, Yiwu Central Hospital, Affiliated Hospital of Wenzhou Medical University, No.699, Jiangdong Road, Yiwu City, 322000, Zhejiang Province, People's Republic of China.

Background: Circular RNAs (circRNAs) play critical roles in the development of atherosclerosis (AS). This study investigated the role of circMTO1 in the progression of AS.

Methods: Serum samples from AS patients and healthy volunteers and vascular smooth muscle cells (VSMCs) were used as the study materials. Read More

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Pericardial-Peritoneal Window as an Alternative Treatment for Large and Recurrent Pericardial Effusion Post-Pericardiotomy.

Braz J Cardiovasc Surg 2021 Jul 7. Epub 2021 Jul 7.

Aerospace Breeding Research Centre of CASTC, Beijing, China.

Introduction: Drug-eluting stents (DES) coated with rapamycin or paclitaxel as antiproliferative substances significantly reduced the incidence of clinical restenosis and had fewer side effects after percutaneous coronary intervention. However, DES coated with rapamycin or paclitaxel still cause restenosis due to abnormal tissue growth which remained a therapeutic problem, particularly in certain subgroups, possibly due to drug concentrations. This study examined the impact of different concentrations of rapamycin and paclitaxel on cytokine, cell viability and proliferation in human aortic smooth muscle cells (HASMC)-derived foam cells. Read More

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Oxidized-Desialylated Low-Density Lipoprotein Inhibits the Antitumor Functions of Lymphokine Activated Killer Cells.

J Cancer 2021 16;12(16):4993-5004. Epub 2021 Jun 16.

School of Molecular Sciences and The Biodesign Institute - Center for Personalized Diagnostics, Arizona State University, P.O. Box 876401, Tempe, AZ 85287, USA.

Elevated concentrations of circulating low density lipoprotein (LDL) that is abnormally oxidized and desialylated is both a precursor to and a hallmark of atherosclerosis. Peripheral blood mononuclear cells (PBMCs) treated with interleukin-2 (IL-2) become lymphokine activated killer (LAK) cells, the primary effectors of which are NK cells and NKT cells. LAK cells display antitumor functions such as increased cytotoxicity and IFN-γ production, and they have been evaluated as a potential cancer therapeutic. Read More

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passivation of GaInP nanowires using radial AlInP shells grown by MOVPE.

Nanotechnology 2021 Jul 29;32(42). Epub 2021 Jul 29.

Solid State Physics, NanoLund, Lund University, PO Box 118, SE-22100 Lund, Sweden.

GaInP nanowires with suitable bandgap (1.35-2.26 eV) ranging from the visible to near-infrared wavelength have great potential in optoelectronic applications. Read More

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Polymyxins induced metabolic perturbations in human lung epithelial cells.

Antimicrob Agents Chemother 2021 Jul 6:AAC0083521. Epub 2021 Jul 6.

Biomedicine Discovery Institute, Department of Microbiology, Monash University, Victoria, 3800, Australia.

Inhaled polymyxins are associated with toxicity in human lung epithelial cells involving multiple apoptotic pathways. However, the mechanism of polymyxin-induced pulmonary toxicity remains unclear. This study aimed to investigate polymyxin-induced metabolomic perturbations in human lung epithelial A549 cells. Read More

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