308 results match your criteria decreased bioluminescent

Dual-Functional PLGA Nanoparticles Co-Loaded with Indocyanine Green and Resiquimod for Prostate Cancer Treatment.

Int J Nanomedicine 2021 12;16:2775-2787. Epub 2021 Apr 12.

Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Purpose: With the advance of screening techniques, there is a growing number of low-risk or intermediate-risk prostate cancer (PCa) cases, remaining a serious threat to men's health. To obtain better efficacy, a growing interest has been attracted to develop such emerging treatments as immunotherapy and focal therapy. However, few studies offer guidance on whether and how to combine these modalities against PCa. Read More

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Extracellular Matrix Proteins Confer Cell Adhesion-Mediated Drug Resistance Through Integrin α in Glioblastoma Cells.

Front Cell Dev Biol 2021 23;9:616580. Epub 2021 Mar 23.

Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA, United States.

Chemotherapy resistance to glioblastoma (GBM) remains an obstacle that is difficult to overcome, leading to poor prognosis of GBM patients. Many previous studies have focused on resistance mechanisms intrinsic to cancer cells; the microenvironment surrounding tumor cells has been found more recently to have significant impacts on the response to chemotherapeutic agents. Extracellular matrix (ECM) proteins may confer cell adhesion-mediated drug resistance (CAMDR). Read More

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Bioluminescent Tumor Signal Is Mouse Strain and Pelt Color Dependent: Experience in a Disseminated Lymphoma Model.

Mol Imaging Biol 2021 Apr 8. Epub 2021 Apr 8.

Mallinckrodt Institute of Radiology, Washington University School of Medicine, Campus Box 8131, 510 South Kingshighway Boulevard, St Louis, MO, 63110, USA.

Background: Many preclinical cancer studies use mice with varied phenotypes to monitor tumor treatment. We compared survival and optical imaging characteristics of strains with varied coat colors harboring luciferase-expressing disseminated lymphoma.

Results: Luciferase-expressing lymphoma cells (Raji-luc) were injected via tail vein into severe combined immunodeficient (SCID) and Rag2-IL2rg (R2G2) mice, and survival was tracked. Read More

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The G1 phase optical reporter serves as a sensor of CDK4/6 inhibition .

Int J Biol Sci 2021 31;17(3):728-741. Epub 2021 Jan 31.

Cancer Center & Department of Breast and Thyroid Surgery, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian, China.

Visualization of cell-cycle G1 phase for monitoring the early response of cell cycle specific drug remains challenging. In this study, we developed genetically engineered bioluminescent reporters by fusing full-length cyclin E to the C-terminal luciferase (named as CycE-Luc and CycE-Luc2). Next, HeLa cell line or an ER-positive breast cancer cell line MCF-7 was transfected with these reporters. Read More

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January 2021

Regulation of the Glycine Transporter GLYT1 by microRNAs.

Neurochem Res 2021 Jan 23. Epub 2021 Jan 23.

Centro de Biología Molecular Severo Ochoa, Facultad de Ciencias, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, C / Nicolás Cabrera 1, Madrid, 28049, Spain.

The glycine transporter GLYT1 participates in inhibitory and excitatory neurotransmission by controlling the reuptake of this neuroactive substance from synapses. Over the past few years, microRNAs have emerged as potent negative regulators of gene expression. In this report, we investigate the possible regulation of GLYT1 by microRNAs. Read More

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January 2021

Fibroblast growth factor receptor facilitates recurrence of minimal residual disease following trastuzumab emtansine therapy.

NPJ Breast Cancer 2021 Jan 21;7(1). Epub 2021 Jan 21.

Purdue University Center for Cancer Research, Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN, 47907, USA.

Trastuzumab-emtansine (T-DM1) is an antibody-drug conjugate (ADC) that efficiently delivers a highly potent microtubule inhibitor to HER2 overexpressing cells. Herein, we utilize HER2 transformed human mammary epithelial cells (HME2) to demonstrate in vitro and in vivo response and recurrence upon T-DM1 treatment. Continuous in vitro dosing of HME2 cells with T-DM1 failed to produce a spontaneously resistant cell line. Read More

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January 2021

5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems.

PLoS One 2020 14;15(12):e0243747. Epub 2020 Dec 14.

Promega Biosciences, Inc., San Luis Obispo, California, United States of America.

Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings. Solutions of detection reagent, containing a mixture of a substrate and luciferase enzyme that produces photons in the presence of ATP, are relatively unstable and maintain only a limited shelf life even under refrigerated conditions. It is therefore common for the individual performing a hygiene test to manually prepare fresh reagent at the time of monitoring. Read More

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January 2021

Suppression of Methane Generation during Methanogenesis by Chemically Modified Humic Compounds.

Antioxidants (Basel) 2020 Nov 17;9(11). Epub 2020 Nov 17.

Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.

The introduction of various concentrations of chemically modified humic compounds (HC) with different redox characteristics into the media with free and immobilized anaerobic consortia accumulating landfill gases was studied as approach to their functioning management. For this purpose, quinone (hydroquinone, naphthoquinone or methylhydroquinone) derivatives of HC were synthesized, which made it possible to vary the redox and antioxidant properties of HC as terminal electron acceptors in methanogenic systems. The highest acceptor properties were obtained with potassium humate modified by naphthoquinone. Read More

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November 2020

A streamlined proliferation assay using mixed lymphocytes for evaluation of human mesenchymal stem cell immunomodulation activity.

J Immunol Methods 2021 Jan 16;488:112915. Epub 2020 Nov 16.

Blood and Coagulation Research, US Army Institute of Surgical Research, JBSA Fort Sam Houston, TX, United States of America.

Background: Mesenchymal stromal cells (MSCs) have been proposed for treatment of acute respiratory distress syndrome (ARDS), graft versus host disease (GVHD), wound healing and trauma. A consensus is building that immunomodulation by MSCs is important for therapeutic potential. MSCs suppress peripheral blood mononuclear cell (PBMC) proliferation in vitro, potentially reflecting an ability to suppress PBMC inflammatory responses in vivo. Read More

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January 2021

Inhibition of Angiotensin Converting Enzyme Impairs Anti-staphylococcal Immune Function in a Preclinical Model of Implant Infection.

Front Immunol 2020 11;11:1919. Epub 2020 Sep 11.

Department of Orthopaedic Surgery, University of California, Los Angeles, CA, United States.

Evidence suggests the renin-angiotensin system (RAS) plays key immunomodulatory roles. In particular, angiotensin-converting enzyme (ACE) has been shown to play a role in antimicrobial host defense. ACE inhibitors (ACEi) and angiotensin receptor blockers (ARB) are some of the most commonly prescribed medications, especially in patients undergoing invasive surgery. Read More

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[β-arrestin1 overexpression suppresses progression of human T-cell acute lymphatic leukemia Molt-4 cell xenograft in mice].

Nan Fang Yi Ke Da Xue Xue Bao 2020 May;40(5):654-660

Center for Clinical Molecular Medicine, Children's Hospital, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing Engineering Research Center of Stem Cell Therapy, Chongqing 400014, China.

Objective: To investigate the effect of β-arrestin1 overexpression on tumor progression in a NCG mouse model bearing T-cell acute lymphocytic leukemia (T-ALL) Molt-4 cell xenograft.

Methods: Molt-4 cells were tagged with firefly-luciferase (F-Luc) by lentiviral infection, and fluorescence intensity of the cells was detected using a luminescence detector. Molt-4 cell lines with β-arrestin1 overexpression or knockdown were constructed by lentivirus infection and injected the tail vein in sub-lethal irradiated NCG mice. Read More

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Seasonal Antimicrobial Activity of the Airway: Post-Hoc Analysis of a Randomized Placebo-Controlled Double-Blind Trial.

Nutrients 2020 Aug 27;12(9). Epub 2020 Aug 27.

Department of Internal Medicine, Carver College of Medicine Iowa City, University of Iowa, Iowa City, IA 52242, USA.

Background: It is widely unknown why respiratory infections follow a seasonal pattern. Variations in ultraviolet B (UVB) light during seasons affects cutaneous synthesis of vitamin D. Serum vitamin D concentration influences the expression of airway surface liquid (ASL) antimicrobial peptides such as LL-37. Read More

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Tumor neoantigen heterogeneity impacts bystander immune inhibition of pancreatic cancer growth.

Transl Oncol 2020 Dec 28;13(12):100856. Epub 2020 Aug 28.

Division of Pharmacoengineering and Molecular Pharmaceutics, Center for Nanotechnology in Drug Delivery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address:

The immunogenic clonal-fraction threshold in heterogeneous solid-tumor required to induce effective bystander-killing of non-immunogenic subclones is unknown. Pancreatic cancer poses crucial challenges for immune therapeutic interventions due to low mutational-burden and consequent lack of neoantigens. Here, we designed a model to incorporate artificial-neoantigens into genes-of -interest in cancer-cells and to test their potential to actuate bystander-killing. Read More

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December 2020

Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of Platelets.

Pharmaceuticals (Basel) 2020 Aug 1;13(8). Epub 2020 Aug 1.

Therapeutic Department of Institute of Postgraduate Education, Krasnoyarsk State Medical University Named After Prof. V.F. Voyno-Yaseneckiy, 660125 Krasnoyarsk, Russia.

Sensitivity to acetylsalicylic acid (ASA) is important in the treatment of patients with coronary heart disease (CHD) after coronary artery bypass grafting (CABG). Patients were divided into ASA sensitive (sASA) and ASA resistant (rASA) by the activity of platelet aggregation induced arachidonic acid (ARA) together with ASA. Induced platelet aggregation activity was studied in sASA and rASA patients with CHD before and after CABG. Read More

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Stability of d-luciferin for bioluminescence to detect gene expression in freely moving mice for long durations.

Luminescence 2021 Feb 8;36(1):94-98. Epub 2020 Aug 8.

Department of Pharmaceutical Sciences, International University of Health and Welfare, Ohtawara, Tochigi, Japan.

Circadian disturbance of clock gene expression is a risk factor for diseases such as obesity, cancer, and sleep disorders. To study these diseases, it is necessary to monitor and analyze the expression rhythm of clock genes in the whole body for a long duration. The bioluminescent reporter enzyme firefly luciferase and its substrate d-luciferin have been used to generate optical signals from tissues in vivo with high sensitivity. Read More

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February 2021

A biosensor for measuring NAD levels at the point of care.

Nat Metab 2019 12 13;1(12):1219-1225. Epub 2019 Dec 13.

Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany.

The cellular level of nicotinamide adenine dinucleotide (NAD), through its different functions, affects cellular metabolism and signalling. A decrease in the NAD content has been associated with various pathologies and physiological aging, while strategies to boost cellular NAD levels have been shown to be effective against age-related diseases in many animal models. The link between decreased NAD levels and numerous pathologies and physiological aging has triggered the need for a simple quantification method for NAD, ideally applicable at the point of care. Read More

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December 2019

Estimation of Hg(II) in Soil Samples by Bioluminescent Bacterial Bioreporter ARL1, and the Effect of Humic Acids and Metal Ions on the Biosensor Performance.

Sensors (Basel) 2020 Jun 2;20(11). Epub 2020 Jun 2.

Institute of Geology of the Czech Academy of Sciences, Rozvojová 269, CZ-16500 Prague 6, Czech Republic.

Mercury is a ubiquitous environmental pollutant of dominantly anthropogenic origin. A critical concern for human health is the introduction of mercury to the food chain; therefore, monitoring of mercury levels in agricultural soil is essential. Unfortunately, the total mercury content is not sufficiently informative as mercury can be present in different forms with variable bioavailability. Read More

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Impact of hypoxia on chemoresistance of mesothelioma mediated by the proton-coupled folate transporter, and preclinical activity of new anti-LDH-A compounds.

Br J Cancer 2020 08 4;123(4):644-656. Epub 2020 Jun 4.

Department of Medical Oncology, Cancer Center Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.

Background: Expression of proton-coupled folate transporter (PCFT) is associated with survival of mesothelioma patients treated with pemetrexed, and is reduced by hypoxia, prompting studies to elucidate their correlation.

Methods: Modulation of glycolytic gene expression was evaluated by PCR arrays in tumour cells and primary cultures growing under hypoxia, in spheroids and after PCFT silencing. Inhibitors of lactate dehydrogenase (LDH-A) were tested in vitro and in vivo. Read More

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Bacterial adhesion inhibitor prevents infection in a rodent surgical incision model.

Virulence 2020 01;11(1):695-706

Department of Microbiology and Molecular Genetics, University of Texas Health Science Center at Houston, McGovern Medical School , Houston, TX, USA.

Surgical site infection risk continues to increase due to lack of efficacy in current standard of care drugs. New methods to treat or prevent antibiotic-resistant bacterial infections are needed. Multivalent Adhesion Molecules (MAM) are bacterial adhesins required for virulence. Read More

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January 2020

CC12 Induces Apoptotic Cell Death and Cell Cycle Arrest in Human Glioblastoma Cell Lines and Mouse Xenograft Model.

Molecules 2020 Apr 14;25(8). Epub 2020 Apr 14.

Department of Neurosurgery, Taipei City Hospital, Taipei 10684, Taiwan.

Among central nervous system tumors, glioblastoma (GBM) is the most common and the most malignant type. Even under current standard treatments, the overall survival rate is still low and the recurrence rate is high. Therefore, developing novel and effective therapy is urgently needed. Read More

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Drug resistance occurred in a newly characterized preclinical model of lung cancer brain metastasis.

BMC Cancer 2020 Apr 7;20(1):292. Epub 2020 Apr 7.

Department of Basic Pharmaceutical Sciences, School of Pharmacy, West Virginia University, 108 Biomedical Drive, Morgantown, WV, 26506, USA.

Background: Cancer metastasis and drug resistance have traditionally been studied separately, though these two lethal pathological phenomena almost always occur concurrently. Brain metastasis occurs in a large proportion of lung cancer patients (~ 30%). Once diagnosed, patients have a poor prognosis surviving typically less than 1 year due to lack of treatment efficacy. Read More

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A new tyrosine kinase inhibitor K905-0266 inhibits proliferation and sphere formation of glioblastoma cancer cells.

J Drug Target 2020 11 7;28(9):933-938. Epub 2020 Jul 7.

Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.

Glioblastoma (GBM) is the most prevalent malignant tumour of the central nervous system and carries a poor prognosis; average survival time after diagnosis is 14 months. Because of its unfavourable prognosis, novel therapies are needed. The aim of this study was to assess whether inhibition of GBM and GBM-derived cancer stem cells (CSCs) by a new tyrosine kinase inhibitor (TKI), K905-0266, is possible. Read More

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November 2020

vβ3 Integrin Antagonists Enhance Chemotherapy Response in an Orthotopic Pancreatic Cancer Model.

Front Pharmacol 2020 27;11:95. Epub 2020 Feb 27.

Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, NY, United States.

Pancreatic cancer decreases survival time and quality of life because of drug resistance and peripheral neuropathy during conventional treatment. This study was undertaken to investigate whether αvβ3 integrin receptor antagonist compounds NDAT and XT199 can suppress the development of cisplatin resistance and cisplatin-induced peripheral neuropathy in an orthotopic pancreatic SUIT2-luc cancer cell mouse model. Anticancer effects of these compounds and their combination with cisplatin were assessed in this tumor mouse model with bioluminescent signaling and histopathology, and a cytokine assay was used to examine expression of inflammatory cytokines IL-1β, IL-6, IL-10, and TNF-α from plasma samples. Read More

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February 2020

Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.

Hum Reprod 2020 03;35(3):494-503

Institute of Life Science and School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, PR China.

Study Question: Does lysine 2-hydroxyisobutyrylation, a newly identified protein posttranslational modification (PTM), occur in human sperm and affect human sperm function?

Summary Answer: Lysine 2-hydroxyisobutyrylation mainly occurs in human sperm tail proteins, and excessive lysine 2-hydroxyisobutyrylation affects human sperm motility.

What Is Known Already: PTM is regarded as an important pathway in regulating sperm function since mature sperm are almost transcriptionally silent. However, only phosphorylation was extensively studied in mature sperm to date. Read More

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Altered Mitochondrial Dynamics, Biogenesis, and Functions in the Paclitaxel-Resistant Lung Adenocarcinoma Cell Line A549/Taxol.

Med Sci Monit 2020 Mar 4;26:e918216. Epub 2020 Mar 4.

Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China (mainland).

BACKGROUND Chemoresistance is a primary hindrance for current cancer treatments. The influence of abnormal mitochondria in chemotherapy resistance is not well known. To explore the correlation between mitochondria and acquired chemoresistance, this work studied alterations in mitochondrial dynamics, biogenesis, and functions for paclitaxel-resistant cancer cell line A549/Taxol and its parental line A549. Read More

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Regulations of miR-183-5p and Snail-Mediated Shikonin-Reduced Epithelial-Mesenchymal Transition in Cervical Cancer Cells.

Drug Des Devel Ther 2020 11;14:577-589. Epub 2020 Feb 11.

Laboratory of Tumor Biology, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province 510120, People's Republic of China.

Background: Shikonin, the main ingredient of , has been reported to have antitumor effects via multiple targets and signaling pathways. However, the detailed mechanism underlying the effects in cervical cancer still remained unknown.

Methods: MTT, wound-healing, transwell assays and flow cytometry experiments were used to measure cell growth, migration, invasion, and cell cycle analysis. Read More

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October 2020

An optical detection module-based biosensor using fortified bacterial beads for soil toxicity assessment.

Anal Bioanal Chem 2020 May 18;412(14):3373-3381. Epub 2020 Feb 18.

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, 02841, Republic of Korea.

An optical biosensor module for soil contamination assessment is presented, employing bioluminescent bacterial bioreporters encapsulated in poly-dopamine (PD)-coated alginate microbeads. The PD-coated beads displayed improved mechanical strength and stability, but somewhat delayed responses to the inducing toxicant. Using toluene as a model soil contaminant, two bioluminescent reporter strains were employed for its detection in the ambient light-blocking, temperature-controlled biosensor module. Read More

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In vivo antitumour activity of Britanin against gastric cancer through nuclear factor-κB-mediated immune response.

J Pharm Pharmacol 2020 Apr 14;72(4):607-618. Epub 2020 Jan 14.

Engineering Research Center of Molecular & Neuroimaging, Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an, China.

Objectives: Britanin was explored for the antitumour effect on gastric cancer, which is a sesquiterpene lactone (SL) extracted from Inula japonica.

Methods: In the present study, cell viability assays were performed to evaluate the antiproliferation effect of Britanin on gastric cancer cells. Tumour development in BGC-823 cell-bearing nude mice was monitored in real-time after Britanin treatment via a bioluminescent imaging method. Read More

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Crocetin Extracted from Saffron Shows Antitumor Effects in Models of Human Glioblastoma.

Int J Mol Sci 2020 Jan 9;21(2). Epub 2020 Jan 9.

Laboratory of Radiobiology, Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 67100 L'Aquila, Italy.

Over recent years, many authors discussed the effects of different natural compounds on glioblastoma (GBM). Due to its capacity to impair survival and progression of different cancer types, saffron extract (SE), named crocetin (CCT), is particularly noteworthy. In this work, we elucidated the antitumor properties of crocetin in glioma in vivo and in vitro models for the first time. Read More

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January 2020

Effect of mutation at positively charged residues (K329 and R330) in a flexible region of firefly luciferase on structure and kinetic properties.

Enzyme Microb Technol 2019 Dec 4;131:109424. Epub 2019 Sep 4.

Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Firefly luciferase as a bioluminescent enzyme has many applications in various fields from scientific research to commercial goals. This enzyme is relatively unstable with low functional capacity due to rapid inactivation in physiological temperature, low in vitro stability and high susceptibility to proteolytic degradation. Based on previous studies, two regions 206-220 and 329-341 on N-domain of Photinus pyralis luciferase are known accessible and flexible. Read More

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December 2019