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Lyophilization of premixed COVID-19 diagnostic RT-qPCR reactions enables stable long-term storage at elevated temperature.

Biotechnol J 2021 May 8:e2000572. Epub 2021 May 8.

Department of Chemical and Biological Engineering.

Background: Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) diagnostic tests for SARS-CoV-2 are the cornerstone of the global testing infrastructure. However, these tests require cold-chain shipping to distribute, and the labor of skilled technicians to assemble reactions and interpret the results. Strategies to reduce shipping and labor costs at the point-of-care could aid in diagnostic testing scale-up and response to the COVID-19 outbreak, as well as in future outbreaks. Read More

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Shorter outpatient wait-times for buprenorphine are associated with linkage to care post-hospital discharge.

Drug Alcohol Depend 2021 Apr 20;224:108703. Epub 2021 Apr 20.

Department of Medicine, Boston University School of Medicine, 801 Massachusetts Ave, Boston, MA, 02118, USA.

Background: Inpatient addiction consult services (ACS) lower barriers to accessing medications for opioid use disorder (MOUD), however not every patient recommended for MOUD links to outpatient care. We hypothesized that fewer days between discharge date and outpatient appointment date was associated with improved linkage to buprenorphine treatment among patients evaluated by an ACS.

Methods: We extracted appointment and demographic data from electronic medical records and conducted retrospective chart review of adults diagnosed with opioid use disorder (OUD) evaluated by an ACS in Boston, MA between July 2015 and August 2017. Read More

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Forecasting emergency department hourly occupancy using time series analysis.

Am J Emerg Med 2021 Apr 29;48:177-182. Epub 2021 Apr 29.

Department of Statistics and Operations Research, University of North Carolina at Chapel Hill (UNC), NC, USA. Electronic address:

Study Objective: To develop a novel predictive model for emergency department (ED) hourly occupancy using readily available data at time of prediction with a time series analysis methodology.

Methods: We performed a retrospective analysis of all ED visits from a large academic center during calendar year 2012 to predict ED hourly occupancy. Due to the time-of-day and day-of-week effects, a seasonal autoregressive integrated moving average with external regressor (SARIMAX) model was selected. Read More

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Hydrothermally-altered feldspar as an environmentally-friendly technology to promote heavy metals immobilization: Batch studies and application in smelting-affected soils.

J Environ Manage 2021 May 5;291:112711. Epub 2021 May 5.

School of Agricultural Sciences, Department of Soil Science, Federal University of Lavras, Lavras, MG, Brazil. Electronic address:

Hydrothermally-altered feldspar (HydroPotash, HYP) possesses, among other physicochemical properties, high pH buffering and cation exchange capacity. Therefore, it may potentially remove heavy metals from aqueous solutions and immobilize these metals in contaminated soil. This study aimed to evaluate the capabilities of two types of HydroPotash (HYP-1 and HYP-2) and a zeolite sample (a commercial adsorbent) for immobilizing cadmium (Cd), zinc (Zn), and lead (Pb) from both aqueous solution and contaminated soils from a Zn-smelting area (classified as soil, soil, and soil based on their level of soluble metal concentration). Read More

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Platelet Function Monitoring Performed after Carotid Stenting during Endovascular Stroke Treatment Predicts Outcome.

J Stroke Cerebrovasc Dis 2021 May 4;30(7):105800. Epub 2021 May 4.

Stroke Unit, Neurology Unit, Department of Neuroscience, Ospedale Civile, Azienda Ospedaliera Universitaria di Modena, Via Giardini 1355, Modena, Emilia Romagna 41126, Italy. Electronic address:

Objectives: Many studies showed that platelet reactivity testing can predict ischemic events after carotid stenting or ischemic stroke. The aim of our study was to assess the role of early platelet function monitoring in predicting 90-days functional outcome, stent thrombosis and hemorrhagic transformation in patients with ischemic stroke treated with endovascular procedures requiring emergent extracranial stenting.

Materials And Methods: We performed a retrospective study on consecutive patients with acute anterior circulation stroke admitted to our hospital between January 2015 and March 2020, in whom platelet reactivity testing was performed within 10 days from stenting. Read More

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Early warning of infection in patients undergoing hematopoietic stem cell transplantation using heart rate variability and serum biomarkers.

Transplant Cell Ther 2021 May 5. Epub 2021 May 5.

Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada; Ottawa Hospital Research Institute, Ottawa, ON, Canada; Departments of Critical Care Medicine and Surgery, The Ottawa Hospital, Ottawa, ON, Canada.

Background: Early warning of infection is critical to reduce risk of deterioration and mortality, especially in neutropenic patients following hematopoietic stem cell transplantation (HCT). Given that heart rate variability (HRV) is a sensitive and early marker for infection and serum inflammatory biomarkers can have high specificity for infection, we hypothesized their combination may be useful for accurate early warning of infection.

Purpose: Develop and evaluate a composite predictive model utilizing continuous HRV with daily serum biomarker measurements to provide risk stratification of future deterioration in HCT patients. Read More

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Cryopreservation of unrelated hematopoietic stem cells from a blood and marrow donor bank during the COVID-19 pandemic. A nationwide survey by the Japan Marrow Donor Program.

Transplant Cell Ther 2021 May 5. Epub 2021 May 5.

Aichi Medical University School of Medicine, the Japan Marrow Donor Program.

Background: During the COVID-19 pandemic, donor hematopoietic stem cell grafts are frequently cryopreserved to ensure the availability of graft before starting a conditioning regimen. However, the safety of cryopreservation has been controversial in unrelated hematopoietic stem cell transplantation, especially for bone marrow grafts. In addition, in unrelated HSCT, the effect of the time from harvest to cryopreservation of donor grafts required for the transportation of donor graft has not been fully clarified. Read More

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Association of Early White Blood Cell Trend with Outcomes in Aneurysmal Subarachnoid Hemorrhage.

World Neurosurg 2021 May 5. Epub 2021 May 5.

Department of Neurology, Rhode Island Hospital, Warren Alpert Medical School of Brown University, Providence, RI.

Background: An increasing white blood cell (WBC) count in early course of aneurysmal subarachnoid hemorrhage (SAH) can indicate a systemic inflammatory state triggered by the initial insult. We sought to determine the significance of the early WBC trend as a potential predictor of outcomes.

Methods: We analyzed a cohort of consecutive patients with aneurysmal SAH. Read More

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Delayed egress of CD8 T cells contributes to long-term pathology after ischemic stroke in male mice.

Brain Behav Immun 2021 May 5. Epub 2021 May 5.

Department of Neurology & Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas, United States; Department of Neurology, Center for Advanced Translational Stroke Science, University of Kentucky, Lexington, Kentucky, USA. Electronic address:

Objective: Stroke is a debilitating disorder with significant annual mortality and morbidity rates worldwide. Immune cells are recruited to the injured brain within hours after stroke onset and can exhibit either protective or detrimental effects on recovery. However, immune cells, including CD8 T cells, persist in the injured brain for weeks, suggesting a longer-term role for the adaptive immune system during functional recovery. Read More

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Intravenous tranexamic acid reduce postoperative drainage and pain after open elbow arthrolysis: A randomized controlled trial.

J Shoulder Elbow Surg 2021 May 5. Epub 2021 May 5.

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, P. R. China. Electronic address:

Background: Open elbow arthrolysis (OEA), which has become an established treatment for post-traumatic elbow stiffness (PTES), requires complete release of contracture tissue and wide excision of ectopic bone, which results in extensive bleeding. The aim of the present study is to evaluate the efficacy of intravenous tranexamic acid (TXA) on postoperative drainage, calculated blood loss and early clinical outcomes in patients undergoing OEA.

Methods: A double-blind, randomized, placebo-controlled trial including 96 patients undergoing OEA was undertaken. Read More

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Lactobacillus rhamnosus GG defense against Salmonella enterica serovar Typhimurium infection through modulation of M1 macrophage polarization.

Microb Pathog 2021 May 5:104939. Epub 2021 May 5.

College of Veterinary Medicine, Jilin Agricultural University, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Changchun, China. Electronic address:

Lactobacillus rhamnosus GG (LGG), a model probiotic strain, plays an important role in immune regulatory activity to prevent and treat intestinal inflammation or diarrhea. However, the effect of the immune modulation of LGG on macrophages to prevent Salmonella infection has not been thoroughly studied. In this study, C57BL/6 mice were pre-administered LGG for 7 days continuously, and then infected with Salmonella entericasubsp. Read More

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Baicalin promotes hippocampal neurogenesis via the Wnt/β-catenin pathway in a chronic unpredictable mild stress-induced mouse model of depression.

Biochem Pharmacol 2021 May 5:114594. Epub 2021 May 5.

Hebei University of Chinese Medicine, Shijiazhuang 050200, China; Hebei Province Academy of Chinese Medicine Sciences, Shijiazhuang 050031, China. Electronic address:

Hippocampal neurogenesis is known to be related to depressive symptoms. Increasing evidence indicates that Wnt/β-catenin signaling regulates multiple aspects of adult hippocampal neurogenesis. Baicalin is a major flavonoid compound with multiple pharmacological effects such as anti-inflammatory, anti-apoptotic, and neuroprotective effects. Read More

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Validation of a novel diagnostic test for assessing the risk of urinary uric acid crystallization.

Clin Chim Acta 2021 May 5. Epub 2021 May 5.

Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS-IdISBa), University of Balearic Islands, Palma de Mallorca 07122, Spain. Electronic address:

Background And Aims: Uric acid (UA) kidney stones account for 10 to 11% of all kidney stones, and this percentage has increased over time. An accurate, rapid, simple, and low-cost test is needed to distinguish urine that is susceptible and resistant to the formation of UA crystals. The aim of this paper is to develop a test to assess the risk for UA crystallization (RUAC) and to validate its utility in routine clinical practice by analysis of urine samples of UA stone formers and healthy volunteers. Read More

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Ischemic vascular disease and long-term mortality in emergency abdominal surgical patients: a population-based cohort study.

Acta Anaesthesiol Scand 2021 May 8. Epub 2021 May 8.

Herlev Anaesthesia Critical and Emergency Care Science Unit (ACES) Department of Anaesthesiology, Copenhagen University Hospital Herlev-Gentofte, Herlev, Denmark.

Background: Emergency abdominal surgery carries a high mortality, as patients are often frail with significant comorbidity. We aimed to evaluate the association between co-existing ischemic vascular disease (IVD) and long-term mortality in patients undergoing emergency abdominal surgery.

Methods: We included adult emergency abdominal surgical patients operated on 13 Danish hospitals between January 1 2009 and December 31 2010. Read More

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A Randomized Phase 1 Safety, Pharmacokinetic and Pharmacodynamic Study of the Novel Myostatin Inhibitor Apitegromab (SRK-015): A Potential Treatment for Spinal Muscular Atrophy.

Adv Ther 2021 May 8. Epub 2021 May 8.

Medpace Clinical Pharmacology, Cincinnati, OH, USA.

Introduction: Apitegromab (SRK-015) is an anti-promyostatin monoclonal antibody under development to improve motor function in patients with spinal muscular atrophy, a rare neuromuscular disease. This phase 1 double-blind, placebo-controlled study assessed safety, pharmacokinetic parameters, pharmacodynamics (serum latent myostatin), and immunogenicity of single and multiple ascending doses of apitegromab in healthy adult subjects.

Methods: Subjects were administered single intravenous ascending doses of apitegromab of 1, 3, 10, 20, 30 mg/kg or placebo, and multiple intravenous ascending doses of apitegromab of 10, 20, 30 mg/kg or placebo. Read More

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Supplementation of the diets with hydroponic maize fodder affects digestibility, puberty, sexual behavior, and semen characteristics in buck kids.

Trop Anim Health Prod 2021 May 8;53(2):310. Epub 2021 May 8.

Livestock Farm Complex, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, 600 051, India.

Hydroponics is one of the widely adopted technologies for fodder production in arid and semi-arid regions. In addition to the benefits such as shorter growth period and minimal water and land use, it also produces nutritious fodder that contains essential nutrients required for the growth and reproduction of livestock. This study was conducted to evaluate the effect of feeding hydroponic maize fodder on the reproductive performance of buck kids. Read More

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Biopsy of the same organ within 30 days: a potential radiology performance measure.

Abdom Radiol (NY) 2021 May 8. Epub 2021 May 8.

Department of Radiology, Michigan Medicine, Ann Arbor, MI, USA.

Purpose: To assess the potential value of repeat image-guided biopsy within 30 days as a radiology performance metric.

Methods: This was a HIPAA-compliant IRB-approved retrospective cohort study of all consecutive ultrasound- and CT-guided core biopsies of the chest, abdomen, and pelvis performed at one institution November 2016 to June 2020. The inclusion criterion was repeat biopsy of the same organ within 30 days of the initial biopsy. Read More

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The cognitive and behavioral effects of D-amphetamine and nicotine sensitization in adult zebrafish.

Psychopharmacology (Berl) 2021 May 8. Epub 2021 May 8.

School of Pharmacy and Biomedical Science, University of Portsmouth, St Michael's Building, White Swan Road, Portsmouth, PO1 2DT, UK.

Background: Zebrafish are growing in use as a model for understanding drug dependence and addiction. Sensitization paradigms have been a useful tool in identifying mechanisms involved in drug-induced behavioral and neurological changes, but in zebrafish have tended to focus on locomotor, rather than cognitive, endpoints.

Methods: Here, we used a novel method, the FMP Y-maze, which measures continuous performance through a series of repeated binary choices (L vs R), to establish a model for assessing parameters associated with psychostimulant-induced behavioral and cognitive sensitization in adult zebrafish. Read More

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External validation of the modified LACE+, LACE+, and LACE scores to predict readmission or death after hospital discharge.

J Eval Clin Pract 2021 May 8. Epub 2021 May 8.

Centre for Health Evaluation & Outcome Sciences (CHÉOS), Vancouver, Canada.

Background: Unplanned hospital readmissions are common adverse events. The LACE+ score has been used to identify patients at the highest risk of unplanned readmission or death, yet the external validity of this score remains uncertain.

Methods: We constructed a cohort of patients admitted to hospital between 1 October 2014 and 31 January 2017 using population-based data from British Columbia (Canada). Read More

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Perioperative Management Of Children With Neuromuscular Disorders Based On A Common Protocol: A Prospective, National Study In Italy.

Acta Anaesthesiol Scand 2021 May 8. Epub 2021 May 8.

Department of Emergency Medicine, Anaesthesia and Intensive Care, University Hospital Agostino Gemelli IRCCS, Rome, Italy.

Background: Children with neuromuscular diseases (NMDs) often display respiratory muscle weakness which increases the risk of postoperative pulmonary complications (PPCs) after general anaesthesia. Non-invasive ventilation (NIV) associated with mechanical insufflation-exsufflation (MI-E) can reduce the incidence and severity of PPCs. The aim of this study was to report our experience with a shared peri-operative protocol that consists in using NIV combined with MI-E to improve the postoperative outcome of NMD children (IT-NEUMA-Ped). Read More

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Evaluating the Contribution of Patient-Provider Communication and Cancer Diagnosis to Racial Disparities in End-of-Life Care Among Medicare Beneficiaries.

J Gen Intern Med 2021 May 7. Epub 2021 May 7.

Department of Health Policy, Vanderbilt University School of Medicine, Nashville, TN, USA.

Background: The quality of end-of-life (EOL) care in the USA remains suboptimal, with significant variations in care by race and across disease subgroups. Patient-provider communication may contribute to racial and disease-specific variations in EOL care outcomes.

Objective: We examined racial disparities in EOL care, by disease group (cancer vs. Read More

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Self-reported smell and taste recovery in coronavirus disease 2019 patients: a one-year prospective study.

Eur Arch Otorhinolaryngol 2021 May 7. Epub 2021 May 7.

Department of Medical, Surgical and Health Sciences, Section of Otolaryngology, University of Trieste, Trieste, Italy.

Purpose: The aim of the present study was to estimate the 1 year prevalence and recovery rate of self-reported chemosensory dysfunction in a series of subjects with previous mild-to-moderate symptomatic COVID-19.

Methods: Prospective study based on the SNOT-22, item "sense of smell or taste" and additional outcomes.

Results: 268/315 patients (85. Read More

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Long-term fasting improves lipoprotein-associated atherogenic risk in humans.

Eur J Nutr 2021 May 7. Epub 2021 May 7.

Buchinger Wilhelmi Clinic, Wilhelm-Beck-Straße 27, 88662, Überlingen, Germany.

Purpose: Dyslipidemia is a major health concern associated with an increased risk of cardiovascular mortality. Long-term fasting (LF) has been shown to improve plasma lipid profile. We performed an in-depth investigation of lipoprotein composition. Read More

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Improving Community Care for Patients Discharged from Hospital Through Zone Wide Implementation of a Seamless Care Transition Policy.

Int J Qual Health Care 2021 May 8. Epub 2021 May 8.

Department of Psychiatry, Faculty of Medicine and Dentistry, University of Alberta.

Background: Several studies within the psychiatry literature have illustrated the importance of discharge planning and execution, as well as accessibility of outpatient follow-up post-discharge. We report the results of implementing a new seamless care transition policy to expedite post-discharge follow-up in the community Addiction and Mental Health (AMH) program in the Edmonton Zone, Alberta, Canada. The policy involved a distribution mechanism for assessment by a Mental Health Therapist (MHT) within 7 days of discharge as well as a dedicated roster of community psychiatrists to accept newly discharged patients. Read More

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GENDER DIFFERENCES IN COMPLICATIONS FOLLOWING CATHETER ABLATION OF ATRIAL FIBRILLATION.

Eur Heart J Qual Care Clin Outcomes 2021 May 7. Epub 2021 May 7.

School of Clinical Medicine, Faculty of Medicine, The University of Queensland, Queensland, Australia.

Aim: Population studies that provide unbiased estimates of gender differences in risk of complications following catheter ablation of atrial fibrillation (AF) are sparse. We sought to evaluate the association of female gender and risk of complications following AF ablation in a nation-wide cohort.

Methods And Results: We identified 35,211 patients (29. Read More

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Health economic analysis of total extraperitoneal repair versus Lichtenstein surgery for inguinal hernia: data from a randomized clinical trial.

BJS Open 2021 May;5(3)

Department of Surgery, CLINTEC, Karolinska Institute, Enköping Hospital, Sweden.

Background: The aim was to compare cost-effectiveness of Lichtenstein under local anaesthesia (LLA) with total extraperitoneal repair (TEP) under general anaesthesia for primary inguinal hernia in men. An endoscopic approach to inguinal hernia repair is often considered costlier. The cost of endoscopic hernia repair, however, has not been compared to open inguinal hernia repair in a cost-effective setting. Read More

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Automated control of oxygen titration in preterm infants on non-invasive respiratory support.

Arch Dis Child Fetal Neonatal Ed 2021 May 7. Epub 2021 May 7.

School of Engineering, University of Tasmania College of Sciences and Engineering, Hobart, Tasmania, Australia.

Objective: To evaluate the performance of a rapidly responsive adaptive algorithm (VDL1.1) for automated oxygen control in preterm infants with respiratory insufficiency.

Design: Interventional cross-over study of a 24-hour period of automated oxygen control compared with aggregated data from two flanking periods of manual control (12 hours each). Read More

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Antimicrobial stewardship solutions with a smart innovative tool.

J Am Pharm Assoc (2003) 2021 Apr 24. Epub 2021 Apr 24.

Background: Antimicrobial consumption has been increasing lately. Hence, effective strategies are required to control antimicrobial use and decrease the development of antimicrobial resistance.

Objective: To evaluate the impact of the use of a mobile app on the implementation of antimicrobial stewardship program (ASP) interventions. Read More

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Preoperative chronic opioid use and its impact on early complications in bariatric surgery: a Swedish nationwide cohort study of 56,183 patients.

Surg Obes Relat Dis 2021 Apr 20. Epub 2021 Apr 20.

Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.

Background: The association between severe obesity and chronic pain makes opioid use common among bariatric patients. Preoperative opioid use has been identified as a risk factor in other surgical procedures.

Objectives: To examine the impact of preoperative opioid use on complications after primary bariatric surgery. Read More

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Preparation and evaluation of an innovative antibacterial bi-layered composite dressing for skin wound healing.

J Tissue Viability 2021 Apr 30. Epub 2021 Apr 30.

Weihai Food and Drug Inspection Testing Center, No.52, Xin Wei Road, Weihai, Shandong Province, 264200, PR China.

Aim Of The Study: The aim of the current study was to develop collagen-based bi-layered composite dressings with antibacterial property and evaluate the efficiency for wound healing.

Materials And Methods: A bi-layered composite wound dressing was fabricated using two marine biomacromolecules (collagen and chitosan or carboxymethyl chitosan). Non-crosslinked and N-Ethyl-N'-(3-dimethylaminopropyl) carbodiimide/N-Hydroxy succinimide (EDC/NHS) cross-linked collagen sponges fabricated by vacuum freeze-drying technology was used as the inner layer. Read More

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