864,607 results match your criteria cytoplasmic


Out of the Dark and Into the Light: A New View of Phytochrome Photobodies.

Front Plant Sci 2021 31;12:732947. Epub 2021 Aug 31.

Donald Danforth Plant Science Center, St. Louis, MO, United States.

Light is a critical environmental stimulus for plants, serving as an energy source via photosynthesis and a signal for developmental programming. Plants perceive light through various light-responsive proteins, termed photoreceptors. Phytochromes are red-light photoreceptors that are highly conserved across kingdoms. Read More

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Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line Originated From in .

Front Plant Sci 2021 31;12:721980. Epub 2021 Aug 31.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

Crosses that lead to heterosis have been widely used in the rapeseed ( L.) industry. Cytoplasmic male sterility (CMS)/restorer-of-fertility () systems represent one of the most useful tools for rapeseed production. Read More

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Identification of Biomarkers for Systemic Distribution of Nanovesicles From N6.2.

Front Immunol 2021 31;12:723433. Epub 2021 Aug 31.

Department of Microbiology and Cell Science, Genetics Institute, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.

The ability of bacterial extracellular vesicles (EV) to transport biological molecules has increased the research to determine their potential as therapeutic agents. In this study, N6.2-derived nanovesicles (NV) were characterized to identify components that may serve as biomarkers in host-microbe interactions. Read More

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A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi.

Sci Rep 2021 Sep 16;11(1):18521. Epub 2021 Sep 16.

Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, People's Republic of China.

Plant kinases containing the LysM domain play important roles in pathogen recognition and self-defense reactions. And it could recognize microbe-associated molecules including chitin and other polypeptides. The white tip nematode Aphelenchoides besseyi is a migratory parasitic nematode that infects plant shoots. Read More

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September 2021

Brick Strex: a robust device built of LEGO bricks for mechanical manipulation of cells.

Sci Rep 2021 Sep 16;11(1):18520. Epub 2021 Sep 16.

BioMediTech, Faculty of Medicine and Health Technology, Tampere University, Arvo Ylpön katu 34, 33520, Tampere, Finland.

Cellular forces, mechanics and other physical factors are important co-regulators of normal cell and tissue physiology. These cues are often misregulated in diseases such as cancer, where altered tissue mechanics contribute to the disease progression. Furthermore, intercellular tensile and compressive force-related signaling is highlighted in collective cell behavior during development. Read More

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September 2021

Activation loop phosphorylaton of a non-RD receptor kinase initiates plant innate immune signaling.

Proc Natl Acad Sci U S A 2021 Sep;118(38)

Institute of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich, 8008 Zurich, Switzerland;

Receptor kinases (RKs) are fundamental for extracellular sensing and regulate development and stress responses across kingdoms. In plants, leucine-rich repeat receptor kinases (LRR-RKs) are primarily peptide receptors that regulate responses to myriad internal and external stimuli. Phosphorylation of LRR-RK cytoplasmic domains is among the earliest responses following ligand perception, and reciprocal transphosphorylation between a receptor and its coreceptor is thought to activate the receptor complex. Read More

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September 2021

Mitochondrial reactive oxygen species trigger metformin-dependent antitumor immunity via activation of Nrf2/mTORC1/p62 axis in tumor-infiltrating CD8T lymphocytes.

J Immunother Cancer 2021 Sep;9(9)

Immunology, Okayama University of Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan

Background: Metformin (Met) is the first-line treatment for type 2 diabetes mellitus and plays an effective role in treating various diseases, such as cardiovascular disease, neurodegenerative disease, cancer, and aging. However, the underlying mechanism of Met-dependent antitumor immunity remains to be elucidated.

Methods: MitoTEMPO, a scavenger of mitochondrial superoxide, abolished the antitumor effect of Met, but not antiprogrammed cell death (PD-1) antibody (Ab) treatment. Read More

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September 2021

Ultra- and micro-structural changes of respiratory tracts in SARS-CoV-2 infected Syrian hamsters.

Vet Res 2021 Sep 16;52(1):121. Epub 2021 Sep 16.

Laboratory of Veterinary Pathology, College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596, South Korea.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is causing a global crisis. It is still unresolved. Although many therapies and vaccines are being studied, they are still in their infancy. Read More

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September 2021

R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells.

Cell Commun Signal 2021 Sep 16;19(1):95. Epub 2021 Sep 16.

Department of Pathology and Laboratory Medicine (SMW, AP, JFL, SLC), MUSC Medical Scientist Training Program (SMW), Medical University of South Carolina, 171 Ashley Avenue, MSC 908, Charleston, SC, 29425-9080, USA.

Background: Loss of the Ras GTPase-activating protein neurofibromin promotes nervous system tumor pathogenesis in patients with neurofibromatosis type 1 (NF1). Neurofibromin loss potentially hyperactivates classic Ras (H-Ras, N-Ras, K-Ras), M-Ras, and R-Ras (R-Ras, R-Ras2/TC21) subfamily proteins. We have shown that classic Ras proteins promote proliferation and survival, but not migration, in malignant peripheral nerve sheath tumor (MPNST) cells. Read More

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September 2021

[Eosinophilic solid and cystic renal cell carcinoma: a clinicopathological analysis of four cases].

Zhonghua Zhong Liu Za Zhi 2021 Sep;43(9):955-958

Department of Pathology, the Second Hospital of Longyan, Longyan 364000, China.

To study the clinicopathological characteristics and prognosis of eosinophilic solid and cystic renal cell carcinoma (ESC RCC). The clinical pathologic data of 4 cases of ESC RCC diagnosed and treated from 2017 to 2019 at the Second Hospital of Longyan City, the Second Affiliated Hospital of Fujian Medical University, and the Zhangzhou Municipal Hospital were collected. The expression of different antibodies was detected by immunohistochemistry, and the diagnosis and differentiate diagnosis were investigated. Read More

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September 2021

Expression and functional characterization of the mannose receptor (MR) from Nile tilapia (Oreochromis niloticus) in response to bacterial infection.

Dev Comp Immunol 2021 Sep 13;126:104257. Epub 2021 Sep 13.

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou 510631, PR China. Electronic address:

Mannose receptor (MR) as a member of the pattern recognition receptors (PRRs) plays an important role in the immune response. In mammals, the role of MR in the regulation of phagocytosis is clarified; however, its contribution to opsonize phagocytosis remains unclear in bony fish. In this study, the expression pattern of Nile tilapia mannose receptor gene (OnMR) was investigated and its regulation of the phagocytosis of monocytes/macrophages to pathogenic bacteria was identified. Read More

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September 2021

Investigation of phage and molasses interactions for the biocontrol of E. coli O157:H7.

Can J Microbiol 2021 Sep 16. Epub 2021 Sep 16.

Hacettepe Universitesi, 37515, Ankara, Turkey, 06800;

Nowadays, resistance in pathogens against antibiotics is one of the most critical health-threatening problems in the world. Therefore, finding new treatment methods to be used as an alternative to antibiotics has become a priority for researchers. Like phages, certain products containing antimicrobial components such as molasses are widely used to eliminate resistant bacteria. Read More

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September 2021

A comprehensive analysis of TDO2 expression in immune cells and characterization of immune cell phenotype in TDO2 knockout mice.

Transgenic Res 2021 Sep 16. Epub 2021 Sep 16.

Institute of Clinical Pharmacology, Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-Inflammatory and Immune Medicine, Anhui Medical University, 81 Meishan Road, Hefei, 230032, China.

Tryptophan 2,3-dioxygenase (TDO2) was an initial rate-limiting enzyme of the kynurenine (Kyn) pathway in tryptophan (Trp) metabolism. We undertook this study to determine a comprehensive analysis of TDO2 expression in immune cells and assess the characterization of immune cell phenotype in TDO2 knockout mice. The expression of TDO2 in various tissues of DBA/1 mice was detected by quantitative real-time PCR (qPCR) and immunohistochemistry. Read More

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September 2021

QTL Mapping of a Novel Genomic Region Associated with High Out-Crossing Rate Derived from Oryza longistaminata and Development of New CMS Lines in Rice, O. sativa L.

Rice (N Y) 2021 Sep 16;14(1):80. Epub 2021 Sep 16.

Novel Gene Resources Laboratory, Strategic Innovation Platform, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.

High seed cost due to poor seed yield severely limits the adoption of hybrid rice by farmers. Increasing the out-crossing rate is one of the key strategies to increase hybrid seed production. Out-crossing rate is highly influenced by the size of female floral traits, which capture pollen grains from male donor plants. Read More

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September 2021

p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease.

Aging Cell 2021 Sep 16:e13434. Epub 2021 Sep 16.

Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia, USA.

MicroRNAs (miRNAs) are small noncoding RNAs ubiquitously expressed in the brain and regulate gene expression at the post-transcriptional level. The nuclear RNase III enzyme Drosha initiates the maturation process of miRNAs in the nucleus. Strong evidence suggests that dysregulation of miRNAs is involved in many neurological disorders including Alzheimer's disease (AD). Read More

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September 2021

V-ATPase subunit a is required for survival and midgut development of Locusta migratoria.

Insect Mol Biol 2021 Sep 16. Epub 2021 Sep 16.

Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China.

The vacuolar-type H -ATPase (V-ATPase) is an ATP-dependent proton pump, which regulates various cellular processes. To date, most functional studies on V-ATPases of insects have focused on subunits of the V1 complex, and there is little information on the VO genes. In this study, two cDNA sequences of LmV-ATPase a were identified in Locusta migratoria. Read More

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September 2021

Palmitoylation of SARS-CoV-2 S protein is critical for S mediated syncytia formation and virus entry.

J Med Virol 2021 Sep 16. Epub 2021 Sep 16.

Department of Clinical Laboratory Medicine, The First Affifiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.

SARS-CoV-2 causes the ongoing COVID-19 pandemic. S protein is the key viral protein for associating with ACE2, the receptor for SARS-CoV-2. There are many kinds of post-translational modifications in S protein. Read More

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September 2021

The interaction of ATP11C-b with ezrin contributes to its polarized localization.

J Cell Sci 2021 Sep 16. Epub 2021 Sep 16.

Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.

ATP11C, a member of the P4-ATPase family, translocates phosphatidylserine and phosphatidylethanolamine at the plasma membrane. We previously revealed that its C-terminal splice variant ATP11C-b exhibits polarized localization in motile cell lines, such as MDA-MB-231 and BaF3. In the present study, we found that the C-terminal cytoplasmic region of ATP11C-b interacts specifically with ezrin. Read More

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September 2021

Pleomorphic liposarcoma with liver metastasis diagnosed by combined fine-needle aspiration cytology and core-needle biopsy.

Diagn Cytopathol 2021 Sep 15. Epub 2021 Sep 15.

Department of Medicine and Surgery, University of Salerno, Salerno, Italy.

Pleomorphic liposarcoma (PLPS) is the rarest liposarcoma subtype, with high-local recurrence and metastasis rates. Fine-needle aspiration cytology (FNAC) is successfully used in the diagnosis of primary or metastatic soft tissue tumors, but liver metastases of PLPS diagnosed by FNAC have never been reported. The cytological diagnosis depends on the identification of lipoblasts with sharply defined cytoplasmic vacuoles indenting and distorting the nucleus in the context of a pleomorphic tumor and in a proper clinical and imaging context. Read More

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September 2021

A Basic Model For Cell Cholesterol Homeostasis.

Traffic 2021 Sep 15. Epub 2021 Sep 15.

Department of Pathology, Rush University Medical Center, Chicago.

Cells manage their cholesterol by negative feedback control using a battery of sterol-responsive proteins. But it is not clear how the abundance and distribution of the sterol is specified. We present a mathematical model that addresses this challenge. Read More

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September 2021

Granulomatosis With Polyangiitis: A Pauci-Immune Rapidly Progressive Glomerulonephritis With Isolated Renal Involvement in an Elderly Male.

Cureus 2021 Aug 11;13(8):e17098. Epub 2021 Aug 11.

Internal Medicine, Mount Sinai Hospital, Chicago, USA.

Granulomatosis with polyangiitis (GPA) is a necrotizing vasculitis with upper and lower respiratory tract and renal system involvement. We present a case of a 59-year-old male presenting with complaints of abdominal pain with deranged renal function and acute increase in creatinine level. On investigation, the antineutrophil cytoplasmic autoantibody, cytoplasmic (c-ANCA) was found to be significantly elevated in association with pauci-immune crescentic glomerulonephritis on biopsy. Read More

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Myosin Heavy Chain 9 (MYH9)-Related Congenital Macrothrombocytopenia.

Cureus 2021 Aug 6;13(8):e16964. Epub 2021 Aug 6.

Oncology, Louisiana State University Health Sciences Center, Shreveport, USA.

Myosin heavy chain 9 (MYH9)-related hereditary macrothrombocytopenia is caused by mutation of the MYH9 gene encoding the heavy chain A of non-muscle myosin of class II. We present a case that emphasizes the importance of awareness of rare disorders which could potentially avoid over-investigation, especially in benign conditions. A 72-year-old Caucasian female presented for preoperative evaluation for cataract extraction. Read More

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Targeting NLRP3 Inflammasome in Translational Treatment of Nervous System Diseases: An Update.

Front Pharmacol 2021 30;12:707696. Epub 2021 Aug 30.

Innovation Research Institute of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.

Neuroinflammatory response is the immune response mechanism of the innate immune system of the central nervous system. Both primary and secondary injury can activate neuroinflammatory response. Among them, the nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome plays a key role in the inflammatory response of the central system. Read More

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In Vitro Modelling of Barrier Impairment Associated with Gastro-Oesophageal Reflux Disease (GERD).

Clin Exp Gastroenterol 2021 8;14:361-373. Epub 2021 Sep 8.

VitroScreen, In Vitro Innovation Center, Milan, 20149, Italy.

Purpose: A novel experimental model based on a 3D reconstructed human oesophageal epithelium model (HO2E) has been developed to investigate the structural and functional changes of the oesophageal epithelium following exposure to a solution of HCl 0.1 N (pH = 1.2) mirroring GERD microenvironment condition. Read More

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September 2021

Review: Cytoplasmic dynein motors in photoreceptors.

Mol Vis 2021 1;27:506-517. Epub 2021 Sep 1.

Department of Ophthalmology, University of Utah Health Science Center, Salt Lake City, UT.

Cytoplasmic dyneins (dynein-1 and dynein-2) transport cargo toward the minus end of microtubules and thus, are termed the "retrograde" cellular motor. Dynein-1 cargo may include nuclei, mitochondria, membrane vesicles, lysosomes, phagosomes, and other organelles. For example, dynein-1 works in the cell body of eukaryotes to move cargo toward the microtubule minus end and positions the Golgi complex. Read More

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September 2021

Jianpi Qushi Heluo Formula alleviates renal damages in Passive Hemann nephritis in rats by upregulating Parkin-mediated mitochondrial autophagy.

Sci Rep 2021 Sep 15;11(1):18338. Epub 2021 Sep 15.

China Department of Nephrology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, 100091, China.

Jianpi Qushi Heluo Formula (JQHF) is an empirical traditional Chinese medicine prescription for treating Membranous Nephropathy (MN) clinically in China. The therapeutic effect of JQHF has been reported in our previous studies. However, the exact mechanism is still unknown. Read More

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September 2021

Cardiomyopathy, Proximal Myopathy, Camptocormia, and Novel Filamin C (FLNC) Variant: A Case Report.

Am J Case Rep 2021 Sep 16;22:e932648. Epub 2021 Sep 16.

Division of Cardiovascular Medicine, School of Medicine, University of Missouri, Columbia, MO, USA.

BACKGROUND Filamin C (FLNC) is an actin crosslinking protein that provides structural support for the sarcomere. The exact function of FLNC is unknown; however, mutations have been reported in myopathies and cardiomyopathies, but rarely both. In this paper, we describe a case of adult-onset camptocormia, proximal myopathy, and cardiomyopathy and an intronic FLNC mutation. Read More

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September 2021

Piloleiomyosarcoma in cats: Histological and immunohistochemical features.

Vet Pathol 2021 Sep 16:3009858211042582. Epub 2021 Sep 16.

University of Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain.

This study describes the histomorphology and immunohistochemical profile of 9 cases of feline piloleiomyosarcoma. Cats ranged in age from 7 to 16 years (mean 10), and tumors were 7 to 24 mm in diameter (mean 15). Tumors were composed of fusiform cells that were haphazardly arranged or in variably sized interwoven bundles. Read More

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September 2021

Ensconsin-dependent changes in microtubule organization and LINC complex-dependent changes in nucleus-nucleus interactions result in quantitatively distinct myonuclear positioning defects.

Mol Biol Cell 2021 Sep 15:mbcE21060324. Epub 2021 Sep 15.

Department of Biology, Boston College, Chestnut Hill, MA 02467, USA.

Nuclear movement is a fundamental process of eukaryotic cell biology. Skeletal muscle presents an intriguing model to study nuclear movement because its development requires the precise positioning of multiple nuclei within a single cytoplasm. Furthermore, there is a high correlation between aberrant nuclear positioning and poor muscle function. Read More

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September 2021

Dual-Modality Poly-l-histidine Nanoparticles to Deliver Peptide Nucleic Acids and Paclitaxel for In Vivo Cancer Therapy.

ACS Appl Mater Interfaces 2021 Sep 15. Epub 2021 Sep 15.

Department of Pharmaceutical Sciences, University of Connecticut, Storrs, Connecticut 06269, United States.

Cationic polymeric nanoformulations have been explored to increase the transfection efficiency of small molecules and nucleic acid-based drugs. However, an excessive positive charge density often leads to severe cell and tissue-based toxicity that restricts the clinical translation of cationic polymeric nanoformulations. Herein, we investigate a series of cationic poly(lactic--glycolic acid) (PLGA)-histidine-based nanoformulations for enhanced cytoplasmic delivery with minimal toxicity. Read More

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September 2021