9,834 results match your criteria cyclophilin

Treatment-Induced BAFF Expression and B Cell Biology in Multiple Sclerosis.

Front Immunol 2021 26;12:676619. Epub 2021 May 26.

Department of Neurosciences, Laboratory for Neuroimmunology, KU Leuven, Leuven, Belgium.

Although fingolimod and interferon-β are two mechanistically different multiple sclerosis (MS) treatments, they both induce B cell activating factor (BAFF) and shift the B cell pool towards a regulatory phenotype. However, whether there is a shared mechanism between both treatments in how they influence the B cell compartment remains elusive. In this study, we collected a cross-sectional study population of 112 MS patients (41 untreated, 42 interferon-β, 29 fingolimod) and determined B cell subsets, cell-surface and RNA expression of BAFF-receptor (BAFF-R) and transmembrane activator and cyclophilin ligand interactor (TACI) as well as plasma and/or RNA levels of BAFF, BAFF splice forms and interleukin-10 (IL-10) and -35 (IL-35). Read More

View Article and Full-Text PDF

Nampt controls skeletal muscle development by maintaining Ca homeostasis and mitochondrial integrity.

Mol Metab 2021 Jun 10:101271. Epub 2021 Jun 10.

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address:

Objective: NAD is a co-factor and substrate for enzymes maintaining energy homeostasis. Nicotinamide phosphoribosyltransferase (NAMPT) controls NAD synthesis, and in skeletal muscle, NAD is important for muscle integrity. However, the underlying molecular mechanisms by which NAD synthesis affects muscle health remain poorly understood. Read More

View Article and Full-Text PDF

The Cyclophilin Regulates ABA-Induced Stomatal Closure and the Drought Stress Response of .

Front Plant Sci 2021 25;12:668792. Epub 2021 May 25.

Key Laboratory of Plant Development and Environmental Adaption Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, China.

Drought causes a major constraint on plant growth, development, and crop productivity. Drought stress enhances the synthesis and mobilization of the phytohormone abscisic acid (ABA). Enhanced cellular levels of ABA promote the production of reactive oxygen species (ROS), which in turn induce anion channel activity in guard cells that consequently leads to stomatal closure. Read More

View Article and Full-Text PDF

Genome-wide characterization of peptidyl-prolyl cis-trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum.

Sci Rep 2021 Jun 10;11(1):12292. Epub 2021 Jun 10.

Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.

Peptidyl-prolyl cis-trans isomerases (PPIases) are the only class of enzymes capable of cis-trans isomerization of the prolyl peptide bond. The PPIases, comprising of different families viz., cyclophilins, FK506-binding proteins (FKBPs), parvulins and protein phosphatase 2A phosphatase activators (PTPAs), play essential roles in different cellular processes. Read More

View Article and Full-Text PDF

In Silico Evaluation of Cyclophilin Inhibitors as Potential Treatment for SARS-CoV-2.

Open Forum Infect Dis 2021 Jun 14;8(6):ofab189. Epub 2021 Apr 14.

Center for Biologics Evaluation and Research, Office of Tissues and Advanced Therapies, Division of Plasma Protein Therapeutics, Food and Drug Administration, Silver Spring, Maryland, USA.

Background: The advent of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) provoked researchers to propose multiple antiviral strategies to improve patients' outcomes. Studies provide evidence that cyclosporine A (CsA) decreases SARS-CoV-2 replication in vitro and decreases mortality rates of coronavirus disease 2019 (COVID-19) patients. CsA binds cyclophilins, which isomerize prolines, affecting viral protein activity. Read More

View Article and Full-Text PDF

Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes.

Sci Rep 2021 Jun 7;11(1):12001. Epub 2021 Jun 7.

Department of Biomedical Sciences and Engineering, National Central University, Taoyuan, Taiwan.

Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. Read More

View Article and Full-Text PDF

Proline/arginine dipeptide repeat polymers derail protein folding in amyotrophic lateral sclerosis.

Nat Commun 2021 06 7;12(1):3396. Epub 2021 Jun 7.

German Center for Neurodegenerative Diseases (DZNE), Göttingen, Germany.

Amyotrophic lateral sclerosis and frontotemporal dementia are two neurodegenerative diseases with overlapping clinical features and the pathological hallmark of cytoplasmic deposits of misfolded proteins. The most frequent cause of familial forms of these diseases is a hexanucleotide repeat expansion in the non-coding region of the C9ORF72 gene that is translated into dipeptide repeat polymers. Here we show that proline/arginine repeat polymers derail protein folding by sequestering molecular chaperones. Read More

View Article and Full-Text PDF

Interplay among Conformation, Intramolecular Hydrogen Bonds, and Chameleonicity in the Membrane Permeability and Cyclophilin A Binding of Macrocyclic Peptide Cyclosporin O Derivatives.

J Med Chem 2021 Jun 7. Epub 2021 Jun 7.

Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.

A macrocyclic peptide scaffold with well-established structure-property relationship is desirable for tackling undruggable targets. Here, we adopted a natural macrocycle, cyclosporin O () and its derivatives (-), and evaluated the impact of conformation on membrane permeability, cyclophilin A (CypA) binding, and the pharmacokinetic (PK) profile. In nonpolar media, showed a similar conformation to cyclosporin A (), a well-known chameleonic macrocycle, but less chameleonic behavior in a polar environment. Read More

View Article and Full-Text PDF

Molecular allergy diagnosis using pollen marker allergens and pollen panallergens: Five patterns seen in multiple test reactions to pollen extracts.

Allergol Select 2021 27;5:180-186. Epub 2021 May 27.

Allergy and Asthma Center Westend, Outpatient Clinic & Research Center Hanf, Ackermann and Kleine-Tebbe, Berlin, Germany.

Polysensitizations to tree, grass, and weed pollen are found in ~ 20% of pollen-allergic individuals. They are often based on broad IgE cross-reactivities to pollen panallergens belonging to highly conserved protein families: 1. profilins, 2. Read More

View Article and Full-Text PDF

Aberrant B Cell Receptor Signaling in Naïve B Cells from Patients with Idiopathic Pulmonary Fibrosis.

Cells 2021 May 26;10(6). Epub 2021 May 26.

Department of Pulmonary Medicine, Erasmus MC, University Medical Center, 3015 GD Rotterdam, The Netherlands.

Idiopathic pulmonary fibrosis (IPF) is a chronic and ultimately fatal disease in which an impaired healing response to recurrent micro-injuries is thought to lead to fibrosis. Recent findings hint at a role for B cells and autoimmunity in IPF pathogenesis. We previously reported that circulating B cells from a fraction of patients, compared with healthy controls, express increased levels of the signaling molecule Bruton's tyrosine kinase (BTK). Read More

View Article and Full-Text PDF

Dysregulation of miR-15a-5p, miR-497a-5p and miR-511-5p Is Associated with Modulation of BDNF and FKBP5 in Brain Areas of PTSD-Related Susceptible and Resilient Mice.

Int J Mol Sci 2021 May 13;22(10). Epub 2021 May 13.

Section of Pharmacology, Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy.

Post-traumatic stress disorder (PTSD) is a neuropsychiatric disorder occurring in susceptible individuals following a traumatic event. Understanding the mechanisms subserving trauma susceptibility/resilience is essential to develop new effective treatments. Increasing evidence suggests that non-coding RNAs, such as microRNAs (miRNAs), may play a prominent role in mediating trauma susceptibility/resilience. Read More

View Article and Full-Text PDF

Nanoparticle-Mediated Simultaneous Targeting of Mitochondrial Injury and Inflammation Attenuates Myocardial Ischemia-Reperfusion Injury.

J Am Heart Assoc 2021 Jun 29;10(12):e019521. Epub 2021 May 29.

Department of Cardiovascular Research Development, and Translational Medicine Center for Disruptive Cardiovascular Innovation Kyushu University Fukuoka Japan.

Background The opening of mitochondrial permeability transition pore and inflammation cooperatively progress myocardial ischemia-reperfusion (IR) injury, which hampers therapeutic effects of primary reperfusion therapy for acute myocardial infarction. We examined the therapeutic effects of nanoparticle-mediated medicine that simultaneously targets mitochondrial permeability transition pore and inflammation during IR injury. Methods and Results We used mice lacking cyclophilin D (CypD, a key molecule for mitochondrial permeability transition pore opening) and C-C chemokine receptor 2 and found that CypD contributes to the progression of myocardial IR injury at early time point (30-45 minutes) after reperfusion, whereas C-C chemokine receptor 2 contributes to IR injury at later time point (45-60 minutes) after reperfusion. Read More

View Article and Full-Text PDF

Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.

Front Physiol 2021 14;12:676864. Epub 2021 May 14.

Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China.

Reverse transcription quantitative real-time PCR is the most commonly used method to detect gene expression levels. In experiments, it is often necessary to correct and standardize the expression level of target genes with reference genes. Therefore, it is very important to select stable reference genes to obtain accurate quantitative results. Read More

View Article and Full-Text PDF

The CypA-netics of Ventilator-Induced Lung Injury.

Am J Respir Crit Care Med 2021 May 25. Epub 2021 May 25.

Institute of Physiology. Charité Universitätsmedizin, Berlin, Germany.

View Article and Full-Text PDF

Combined tacrolimus and melatonin effectively protected kidney against acute ischemia-reperfusion injury.

FASEB J 2021 Jun;35(6):e21661

Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.

Acute kidney injury (AKI) is commonly encountered and causes high mortality in hospitalized patients; however, effective therapies for AKI have still not been established. Accordingly, we performed a rodent model with acute renal ischemia-reperfusion (IR) and tested the hypothesis that combined tacrolimus and melatonin therapy could be superior to either one for protecting the kidney against IR injury. Adult-male SD rat (n = 30) were equally categorized into group 1 (receiving laparotomy only), group 2 (IR treated by 3. Read More

View Article and Full-Text PDF

The combination of the NS5A and cyclophilin inhibitors results in an additive anti-HCV inhibition in humanized mice without development of resistance.

PLoS One 2021 20;16(5):e0251934. Epub 2021 May 20.

Department of Immunology & Microbiology, The Scripps Research Institute, La Jolla, California, United States of America.

We and others previously reported that the direct-acting agents (DAA) NS5A inhibitors (NS5Ai) and the host-targeting agents cyclophilin inhibitors (CypIs) inhibit HCV replication in vitro. In this study, we investigated whether the combination of NS5Ai and CypI offers a potent anti-HCV effect in vivo. A single administration of NS5Ai or CypI alone to HCV-infected humanized-mice inhibits HCV replication. Read More

View Article and Full-Text PDF

Enantioselective Synthesis and Biological Evaluation of Sanglifehrin A and B and Analogs.

Angew Chem Int Ed Engl 2021 May 20. Epub 2021 May 20.


Sanglifehrin A and B are immunosuppressive macrocyclic natural products endowed with and differentiated by a unique spirocyclic lactam. Here, we report an enantioselective total synthesis and biological evaluation of sanglifehrin A and B and analogs. Access to the spirocyclic lactam was achieved via convergent assembly of a key pyranone intermediate followed by a stereo-controlled spirocyclization. Read More

View Article and Full-Text PDF

Cyclophilin A inhibits RSV replication by binding to RSV-N through its PPIase activity.

J Virol 2021 May 19. Epub 2021 May 19.

Department of Immunology, Hebei Medical University, Shijiazhuang, Hebei, China

Human Respiratory syncytial virus (hRSV) is the most common pathogen which causes acute lower respiratory infection (ALRI) in infants. Recently, virus-host interaction is a hot spot of virus related research, and it needs to be further elaborated on its host interactions during RSV infection. Here, we found that RSV infection significantly increased the expression of cypA in clinical patients, mice or epithelial cells. Read More

View Article and Full-Text PDF

Induction of cyclophilin A by influenza A virus infection facilitates group A Streptococcus coinfection.

Cell Rep 2021 May;35(7):109159

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

During influenza A epidemics, bacterial coinfection is a major cause of increased morbidity and mortality. However, the roles of host factors in regulating influenza A virus (IAV)-triggered bacterial coinfection remain elusive. Cyclophilin A (CypA) is an important regulator of infection and immunity. Read More

View Article and Full-Text PDF

Porphyromonas gingivalis lipopolysaccharide and gingival fibroblast augment MMP-9 expression of monocytic U937 cells through cyclophilin A.

J Periodontol 2021 May 17. Epub 2021 May 17.

School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan.

Background: Intercellular cross-talking was suggested in matrix metalloproteinase (MMP)-9 expression with unknown mechanisms. Studies showed cyclophilin A (CypA) playing an important role in regulating MMP-9 expression in varied diseases. The aim of the study was to examine the CyPA on the MMP-9 augmentation in monocytic U937 cells after Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS) treatment and human gingival fibroblast (hGF) co-culture. Read More

View Article and Full-Text PDF

Cyclophilin A Plays Potential Roles in a Rat Model of Asthma and Suppression of Immune Response.

J Asthma Allergy 2021 7;14:471-480. Epub 2021 May 7.

Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.

Purpose: Cyclophilin A (CypA) inhibits CD4 T cell signal transduction via interleukin-2-inducible T-cell kinase (Itk), a tyrosine kinase required for T helper (Th) 2 cells function. Furthermore, mice with CypA silencing developed allergic diseases associated with increased Th2 cytokines production. CD4 T cells with a Th2-cytokine pattern have been demonstrated to have a pivotal role in the pathogenesis of asthma. Read More

View Article and Full-Text PDF

Cyclophilin A regulates secretion of tumour-derived extracellular vesicles.

Transl Oncol 2021 May 10;14(8):101112. Epub 2021 May 10.

UCD School of Biomolecular & Biomedical Science, Conway Institute, University College Dublin (UCD), Belfield, Dublin 4, Ireland. Electronic address:

Extracellular Vesicles (EVs) are a heterogenous population of particles that play an important role in cell-cell communication in physiological and pathophysiological situations. In this study we reveal that the peptidyl prolyl isomerase Cyclophilin A (CypA) is enriched in cancer-derived EVs from a range of haematopoietic malignancies. CypA-enriched blood cancer EVs were taken up by normal monocytes independent of EV surface trypsin-sensitive proteins and potently stimulated pro-inflammatory MMP9 and IL-6 secretion. Read More

View Article and Full-Text PDF

Host factor cyclophilin B affects Orf virus replication by interacting with viral ORF058 protein.

Vet Microbiol 2021 Jul 4;258:109099. Epub 2021 May 4.

Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:

Poxviruses have evolved multiple strategies to modulate host-derived factors to create an optimal environment for viral efficient replication. Our previous study indicated that cyclophilin B (CypB) is a critical factor for ORFV replication in MDBK cells. However, the precise molecular mechanism by which CypB facilitates ORFV replication remains less understood. Read More

View Article and Full-Text PDF

Cyclophilin A induces macrophage apoptosis and enhances atherosclerotic lesions in high-fat diet-fed hyperglycemic rabbits.

FASEB Bioadv 2021 May 1;3(5):305-322. Epub 2021 Mar 1.

Cardiovascular Diseases and Diabetes Biology Rajiv Gandhi Centre for Biotechnology Trivandrum India.

Macrophage apoptosis is a key contributor to the progression of atherosclerosis. Cyclophilin A, a monocyte secretory protein associated with the initiation of atherosclerosis has an inherent nuclease activity. This study reports the mechanism by which cyclophilin A causes apoptosis of macrophages and accelerates the progression of atherosclerosis. Read More

View Article and Full-Text PDF

Current understanding on molecular drug targets and emerging treatment strategy for novel coronavirus-19.

Naunyn Schmiedebergs Arch Pharmacol 2021 May 7. Epub 2021 May 7.

Neuroscience Division, Department of Pharmacology, ISF College of Pharmacy, Moga, 142001, Punjab, India.

SARS-CoV-2 is an enveloped positive-sense RNA virus, contain crown-like spikes on its surface, exceptional of large RNA genome, and a special replication machinery. Common symptoms of SARS-CoV-2 include cough, common cold, fever, sore throat, and a variety of severe acute respiratory disease (SARD) such as pneumonia. SARS-CoV-2 infects epithelial cells, T-cells, macrophages, and dendritic cells and also influences the production and implantation of pro-inflammatory cytokines and chemokines. Read More

View Article and Full-Text PDF

[Corrigendum] Cyclophilin D modulates cell death transition from early apoptosis to programmed necrosis induced by honokiol.

Int J Oncol 2021 Jun 6;58(6). Epub 2021 May 6.

Department of Surgical Oncology, The Second Affiliated Hospital, School of Medicine,Zhejiang University, Hangzhou 310009, P.R. China.

Subsequently to the publication of the above article, the authors have realized that the printed version of Fig. 6 on p. 1661 contained some mistakes. Read More

View Article and Full-Text PDF

Therapeutic effect of SP-8356 on pulmonary embolism-associated cardiac injury is mediated by its ability to suppress apoptosis and inflammation.

J Cell Mol Med 2021 Jun 4;25(11):5260-5268. Epub 2021 May 4.

Department of Cardiovascular Medicine, The First Affiliated Hospital of South China University, Hengyang, China.

The cyclophilin A-CD147 interaction has been reported to be one of the most potential therapeutic targets for the treatment of acute pulmonary embolism. The signalling of extracellular signal-regulated kinase 1/2 (ERK1/2) was also reported in the pathogenesis of cardiac injury. Since SP-8356 is regarded as a novel Inhibitor of CD147-Cyclophilin, the study aimed to evaluate potential therapeutic effects of SP-8356 for pulmonary embolism-associated cardiac injury. Read More

View Article and Full-Text PDF

Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves' disease.

BMC Endocr Disord 2021 Apr 29;21(1):88. Epub 2021 Apr 29.

Department of Hematology, Jiangsu Province Hospital of TCM/the Affiliated Hospital of Nanjing University of Chinese Medicine, 210029, Nanjing, China.

Background: B lymphocyte activating factor (BAFF) is a growth factor regulating B lymphocytes survival and maturation. Serum BAFF levels were elevated in patients affected with autoimmune thyroid diseases (AITD), including Graves' disease (GD) and Hashimoto's thyroiditis (HT). The aim of this study is to explore the association of expression levels of BAFF and its receptors with AITD. Read More

View Article and Full-Text PDF

Cerebral Apolipoprotein D Exits the Brain and Accumulates in Peripheral Tissues.

Int J Mol Sci 2021 Apr 16;22(8). Epub 2021 Apr 16.

Laboratoire du Métabolisme Moléculaire des Lipides, Centre de Recherches CERMO-FC, Département des Sciences Biologiques, Université du Québec à Montréal (UQAM), 141 av. du Président-Kennedy, Montréal, QC H2X 1Y4, Canada.

Apolipoprotein D (ApoD) is a secreted lipocalin associated with neuroprotection and lipid metabolism. In rodent, the bulk of its expression occurs in the central nervous system. Despite this, ApoD has profound effects in peripheral tissues, indicating that neural ApoD may reach peripheral organs. Read More

View Article and Full-Text PDF

Relationship between Brain Tissue Changes and Blood Biomarkers of Cyclophilin A, Heme Oxygenase-1, and Inositol-Requiring Enzyme 1 in Patients with Alzheimer's Disease.

Diagnostics (Basel) 2021 Apr 21;11(5). Epub 2021 Apr 21.

Department of Radiology, Kyung Hee University Hospital at Gangdong, 892 Dongnam-ro, Gangdong-Gu, Seoul 05278, Korea.

Cyclophilin A (CypA), heme oxygenase-1 (HO-1), and inositol-requiring enzyme 1 (IRE1) are believed to be associated with Alzheimer's disease (AD). In this study, we investigated the association between gray matter volume (GMV) changes and blood levels of CypA, HO-1, and IRE1 in cognitively normal (CN) subjects and those with amnestic mild cognitive impairment (aMCI) and AD. Forty-five elderly CN, 34 aMCI, and 39 AD subjects were enrolled in this study. Read More

View Article and Full-Text PDF