1,188,783 results match your criteria cultures 50 µg

Ecotoxicological effects of DBPs on freshwater phytoplankton communities in co-culture systems.

J Hazard Mater 2021 Jul 17;421:126679. Epub 2021 Jul 17.

State Key Laboratory of Urban Water Resource and Environment of Harbin Institute of Technology, Shenzhen 518055, PR China. Electronic address:

Intensive disinfection of wastewater during the COVID-19 pandemic might elevate the generation of toxic disinfection byproducts (DBPs), which has triggered global concerns about their ecological risks to natural aquatic ecosystems. In this study, the toxicity of 17 DBPs typically present in wastewater effluents on three representative microalgae, including Scenedesmus sp. (Chlorophyta), Microcystis aeruginosa (Cyanophyta), and Cyclotella sp. Read More

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A fed-batch feeding with succinic acid strategy for astaxanthin and lipid hyper-production in Haematococcus pluviualis.

Bioresour Technol 2021 Jul 26;340:125648. Epub 2021 Jul 26.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China. Electronic address:

Effects of succinic acid (SA) in fed-batch feeding mode on astaxanthin and lipids biopoduction of Haematococcus pluvialis against abiotic stresses were explored. By comparison with the control, the initial addition of SA on day 0 increased the production of astaxanthin by 71.61%. Read More

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How to grow your cable bacteria: Establishment of a stable single-strain culture in sediment and proposal of Candidatus Electronema aureum GS.

Syst Appl Microbiol 2021 Jul 22;44(5):126236. Epub 2021 Jul 22.

Section for Microbiology, Department of Biology, Aarhus University, Denmark; Center for Electromicrobiology, Aarhus University, Denmark; Center for Geomicrobiology, Aarhus University, Denmark; ECOBE, Department of Biology, University of Antwerp, Belgium.

Cable bacteria are multicellular filamentous bacteria within the Desulfobulbaceae that couple the oxidation of sulfide to the reduction of oxygen over centimeter distances via long distance electron transport (LDET). So far, none of the freshwater or marine cable bacteria species have been isolated into pure culture. Here we describe a method for establishing a stable single-strain cable bacterium culture in partially sterilized sediment. Read More

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Collective behaviours in organoids.

Curr Opin Cell Biol 2021 Jul 28;72:81-90. Epub 2021 Jul 28.

Friedrich Miescher Institute for Biomedical Research (FMI), Maulbeerstrasse 66, 4058 Basel, Switzerland; University of Basel. Petersplatz 1, 4001 Basel, Switzerland. Electronic address:

Collective behaviour emerges from interacting units within communities, such as migrating herds, swimming fish schools, and cells within tissues. At the microscopic level, collective behaviours include collective cell migration in development and cancer invasion, rhythmic gene expression in pattern formation, cell competition in homeostasis and cancer, force generation and mechano-sensing in morphogenesis. Studying the initiation and the maintenance of collective cell behaviours is key to understand the principles of development, regeneration and disease. Read More

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Quantitative microcapillary electrophoresis immunoassay (mCE IA) for end-to-end analysis of pertactin within in-process samples and Quadracel® vaccine.

J Pharm Biomed Anal 2021 Jul 26;204:114284. Epub 2021 Jul 26.

Analytical Sciences Toronto, Sanofi Pasteur Ltd., Toronto, ON, M2R 3T4, Canada. Electronic address:

Protein concentration is an important attribute in the production of subunit or component-based vaccine antigens. Rigorous monitoring of protein concentration is required to identify potential areas for yield improvement. The current GMP method for quantitation is the plate-based ELISA which requires numerous hands-on steps and has low sensitivity in comparison to new microfluidic systems. Read More

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Local and systemic gene expression levels of IL-10, IL-17 and TGF-β in active ocular toxoplasmosis in humans.

Cytokine 2021 Jul 28;146:155643. Epub 2021 Jul 28.

Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Background: To compare mRNA expression of interleukin 10 (IL-10), interleukin 17 (IL-17) and Transforming Growth Factor-β (TGF-β) in aqueous humor (AH) and peripheral blood mononuclear cells (PBMCs) in human ocular toxoplasmosis (OT) and controls.

Method: RNA isolation, cDNA synthesis and real-time polymerase chain reaction were performed on AH sediments and PBMCs of 16 patients with active OT and 21 controls at the Khatam-al-Anbia Eye Hospital, Iran. For comparison, Mann Whitney U test was used at a discrimination level of p < 0. Read More

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Biofilm patterns in gram-positive and gram-negative bacteria.

Microbiol Res 2021 Jul 23;251:126829. Epub 2021 Jul 23.

Department of Biotechnology, Delhi Technological University, Delhi, India.

The Gram-positive and Gram-negative bacteria are attributable to matrix-enclosed aggregates known as biofilms. Biofilms are root cause of industrial biofouling and characterized by antimicrobial resistance during infections. Many biofilm studies examine specific Gram type cultures, whereas nearly all biofilm communities in nature comprise both Gram-negative and Gram-positive bacteria. Read More

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The unwritten history of medical treatment: Evidence for ritual-healers and their activities in the pre-literate past.

Int J Paleopathol 2021 Jul 28;34:206-216. Epub 2021 Jul 28.

De la Préhistoire à l'Actuel: Culture, Environnement, et Anthropologie (PACEA), Université de Bordeaux, UMR 5199 PACEA, Bâtiment B8, Allée Geoffroy Saint-Hilaire, CS 50023, Pessac Cedex, 33615, France. Electronic address:

Objective: To reveal the presence and activities of healers from funerary contexts.

Materials: Ethnohistoric and ethnographic textual descriptions and the bioarchaeological record.

Methods: A synthesis of human remains, grave contexts, and funerary objects. Read More

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Bioconversion of renewable lignocellulosic biomass into multicomponent substrate via pressurized hot water pretreatment for bioplastic polyhydroxyalkanoate accumulation.

Bioresour Technol 2021 Jul 24;339:125667. Epub 2021 Jul 24.

Ministry of Agriculture Key Laboratory of Biology and Genetic Resource Utilization of Rubber Tree/State Key Laboratory Breeding Base of Cultivation & Physiology for Tropical Crops, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China.

The pretreatment of lignocellulosic biomass (LB) has become an important process to reduce the cost of polyhydroxyalkanoate (PHA) production. In this study, an economical and effective pressurized hot water pretreatment was used to investigate on bioconversion of four typical LB (rubber wood, sugarcane bagasse, sorghum stalk, cassava stalk) into reducing sugar, then as feedstock to accumulate PHA by mixed microbial cultures. The results showed that pretreatment changed the surface morphology of LB and further improved saccharification efficiency. Read More

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Characteristics of a novel heterotrophic nitrification-aerobic denitrification yeast, Barnettozyma californica K1.

Bioresour Technol 2021 Jul 26;339:125665. Epub 2021 Jul 26.

School of Life Sciences, South China Normal University, Guangzhou 510631, PR China; Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education, Guangzhou 510631, PR China; Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou 510631, PR China. Electronic address:

Yeast strain K1, isolated from surface sediment, was identified as Barnettozyma californica. The strain showed efficient heterotrophic nitrification and aerobic denitrification (HN-AD) at initial ammonium, nitrite and nitrate concentrations of 14-140 mg/L. Additionally, the optimum carbon source for its growth and nitrogen removal activity was sucrose, followed by glucose, acetate and citrate. Read More

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Autophagic degradation of NOXA underlies stromal cell-mediated resistance to proteasome inhibitors in mantle cell lymphoma.

Leuk Res 2021 Jul 24;111:106672. Epub 2021 Jul 24.

Division of Stem Cell Regulation, Center for Molecular Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. Electronic address:

Mantle cell lymphoma (MCL) is usually resistant to the current standard-of-care regimens and also to novel agents such as the proteasome inhibitor bortezomib. A better prognosis of leukemic variants of MCL suggests that MCL cells acquire drug resistance in nodal and/or bone marrow microenvironments via interaction with supporting cells. Bortezomib exerts cytotoxic action in MCL cells via stabilization of the pro-apoptotic BCL-2 family protein NOXA. Read More

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A toolbox for efficient proximity-dependent biotinylation in zebrafish embryos.

Mol Cell Proteomics 2021 Jul 28:100128. Epub 2021 Jul 28.

Department of Molecular Genetics, University of Toronto, ON, Canada; Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital, Sinai Health, Toronto, ON, Canada, M5G 1X5. Electronic address:

Understanding how proteins are organized in compartments is essential to elucidating their function. While proximity-dependent approaches such as BioID have enabled a massive increase in information about organelles, protein complexes and other structures in cell culture, to date there have been only a few studies in living vertebrates. Here, we adapted proximity labeling for protein discovery in vivo in the vertebrate model organism, zebrafish. Read More

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Proteomics, lipidomics, metabolomics and 16S DNA sequencing of dental plaque from patients with diabetes and periodontal disease.

Mol Cell Proteomics 2021 Jul 28:100126. Epub 2021 Jul 28.

Morgridge Institute for Research, Madison, WI 53715, USA; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; National Center for Quantitative Biology of Complex Systems, Madison, WI 53706, USA. Electronic address:

Oral microbiome influences human health, specifically pre- and type 2 diabetes (Pre-DM/DM) and periodontal diseases (PD), through complex microbial interactions. To explore these relations, we performed 16S rDNA sequencing, metabolomics, lipidomics, and proteomics analyses on supragingival dental plaque collected from individuals with Pre-DM/DM (n=39), Pre-DM/DM and PD (n=37), PD alone (n=11), or neither (n=10). We identified on average 2,790 operational taxonomic units and 2,025 microbial and host proteins per sample and quantified 110 metabolites and 415 lipids. Read More

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Standard therapy of Mycobacterium avium complex pulmonary disease shows limited efficacy in an open source hollow fiber system that simulates human plasma and epithelial lining fluid pharmacokinetics.

Clin Microbiol Infect 2021 Jul 28. Epub 2021 Jul 28.

Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands.

Objectives: Mycobacterium avium complex (MAC) bacteria can cause chronic pulmonary disease (PD). Current treatment regimens of azithromycin, ethambutol and rifampicin have culture conversion rates of around 65%. Dynamic, pre-clinical models to assess the efficacy of treatment regimens are important to guide clinical trial development. Read More

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Advanced human-relevant in vitro pulmonary platforms for respiratory therapeutics.

Adv Drug Deliv Rev 2021 Jul 28:113901. Epub 2021 Jul 28.

Department of Biomedical, Technion - Israel Institute of Technology, 32000 Haifa, Israel. Electronic address:

Over the past years, advanced in vitro pulmonary platforms have witnessed exciting developments that are pushing beyond traditional preclinical cell culture methods. Here, we discuss ongoing efforts in bridging the gap between in vivo and in vitro interfaces and identify some of the bioengineering challenges that lie ahead in delivering new generations of human-relevant in vitro pulmonary platforms. Notably, in vitro strategies using foremost lung-on-chips and biocompatible "soft" membranes have focused on platforms that emphasize phenotypical endpoints recapitulating key physiological and cellular functions. Read More

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Genetically Engineered Mouse Models of Esophageal Cancer.

Exp Cell Res 2021 Jul 28:112757. Epub 2021 Jul 28.

Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Background: Esophageal cancer is the most common cause of cancer-related death worldwide with a diverse geographical distribution, poor prognosis, and diagnosis in advanced stages of the disease. Identification of the mechanisms involved in esophageal cancer development is evaluative to improve outcomes for patients. Genetically engineered mouse models (GEMMs) of cancer provide the physiologic, molecular, and histologic features of the human tumors to determine the pathogenesis and treatments for cancer, hence exhibiting a source of tremendous potential for oncology research. Read More

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The blood neutrophil count after 1 month of treatment predicts the radiological severity of lung disease at treatment end.

Chest 2021 Jul 28. Epub 2021 Jul 28.

Department of Clinical Immunology, Royal Free London NHS Foundation Trust, London NW3 2QG; Institute of Immunity and Transplantation, University College London, Royal Free Campus, London NW3 2QG. Electronic address:

Background: Post-tuberculous lung disease confers significant morbidity. However, the determinants of persistent lung damage in tuberculosis are not well established. We investigated associations between tuberculosis-associated radiological changes and socio-demographic factors, surrogates of bacillary burden and blood inflammatory markers at initiation of therapy and after 1 month. Read More

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Hypothermia Promotes Mitochondrial Elongation In Cardiac Cells Via Inhibition Of Drp1.

Cryobiology 2021 Jul 28. Epub 2021 Jul 28.

Cedars-Sinai Smidt Heart Institute, Barbra Streisand Women's Heart Center, Cedars-Sinai Medical Center, Los Angeles, California, USA.

Hypothermia is a valuable clinical tool in mitigating against the consequences of ischemia in surgery, stroke, cardiac arrest and organ preservation. Protection is afforded principally by a reduction of metabolism, manifesting as reduced rates of oxygen uptake, preservation of ATP levels, and a curtailing of ischemic calcium overload. The effects of non-ischemic hypothermic stress are relatively unknown. Read More

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Feasibility study evaluating a veterinary point-of-care urine culture system for diagnosing septic peritonitis.

J Vet Emerg Crit Care (San Antonio) 2021 Jul 31. Epub 2021 Jul 31.

Department of Microbiology & Immunology, Wake Forest School of Medicine, Winston Salem, North Carolina, USA.

Objective: To evaluate the use of a veterinary point-of-care urine culture system (POCUCS) for the diagnosis of septic peritonitis.

Design: Prospective feasibility study performed between August 2017 and April 2018.

Setting: Private referral hospital. Read More

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Reduced infiltration of T-regulatory cells in tumours from mice fed daily with gamma-tocotrienol supplementation.

Clin Exp Immunol 2021 Jul 31. Epub 2021 Jul 31.

Pathology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.

Gamma-tocotrienol (γT3) is an analogue of vitamin E with beneficial effects on the immune system, including immune-modulatory properties. This study reports the immune-modulatory effects of daily supplementation of γT3 on host T-helper (Th) and T-regulatory (Treg) populations in a syngeneic mouse model of breast cancer. Female BALB/c mice were fed with either γT3 or vehicle (soy oil) for 2-weeks via oral gavage before they were inoculated with syngeneic 4T1 mouse mammary cancer cells (4T1 cells). Read More

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Ocimum Sanctum: An All-Round Treatment for Cancer?

Altern Ther Health Med 2021 Jul 31. Epub 2021 Jul 31.

Background: This review is a great route for future researchers on the topic of alternative medicine. The review goes in depth with respect to Ocimum sanctum and its proposed mechanism of action for different types of cancers.

Primary Study Objective: The primary objective of the study was to bring traditional therapy back, which can prove to be economically beneficial and may be helpful to many patients with cancer with few to no adverse events. Read More

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Protective immunity against tuberculosis in a free-living badger population vaccinated orally with Mycobacterium bovis Bacille Calmette Guérin (BCG).

Transbound Emerg Dis 2021 Jul 31. Epub 2021 Jul 31.

School of Veterinary Medicine, University College Dublin (UCD), Dublin 4, Ireland.

Vaccination of badgers with Mycobacterium bovis Bacille Calmette Guérin (BCG) has been shown to protect badgers against tuberculosis in experimental trials. During the three-year County Kilkenny BCG vaccine field study, badgers were treated orally with placebo (100% in Zone A), BCG (100% in Zone C) or randomly assigned 50%: 50% treatment with BCG or placebo (Zone B). At the end of the study 275 badgers were removed from the trial area and subjected to detailed post-mortem examination followed by histology and culture for M. Read More

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Inner ear organoids: progress and outlook, with a focus on the vascularization.

FEBS J 2021 Jul 31. Epub 2021 Jul 31.

Department of Developmental Biology, University of Science and Culture, Tehran, 1665659911, Iran.

The inner ear is a complex organ that encodes sound, motion and orientation in space. Given the complexity of the inner ear, it is not surprising that treatments are relatively limited despite the fact that, in 2015, hearing loss was the fourth leading cause of years lived with disability (YLDs) worldwide. Inner ear organoid models are a promising tool to advance the study of multiple aspects of the inner ear to aid the development of new treatments and validate drug-based therapies. Read More

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Addressing Gender Inequities: Creation of a Multi-Institutional Consortium of Women Physicians in Academic Emergency Medicine.

Acad Emerg Med 2021 Jul 31. Epub 2021 Jul 31.

Department of Emergency Medicine, Harvard Medical School, USA.

Gender inequity is pervasive in medicine, including emergency medicine (EM), and is well documented in workforce representation, leadership, financial compensation, and resource allocation. The reasons for gender inequities in medicine, including academic EM, are multi-factorial and include disadvantageous institutional parental, family, and promotion policies, workplace environment and culture, implicit biases, and a paucity of women physician leader role models, mentors, and sponsors. To address some of the challenges of gender inequities and career advancement for women in academic EM, we established an innovative, peer-driven, multi-institutional consortium of women EM faculty employed at 4 distinct hospitals affiliated with one medical school. Read More

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Impact of Cell Culture and Copper Dose on Gene Expression in Bovine Liver.

Biol Trace Elem Res 2021 Jul 31. Epub 2021 Jul 31.

Department of Animal Sciences, Colorado State University, Fort Collins, CO, USA.

The objectives of these experiments were to investigate (1) the relative abundance of transcripts for Cu-responsive genes in whole bovine liver vs. cultured hepatocytes and (2) the influence of Cu dose on the relative abundance of transcripts for Cu-responsive genes in cultured bovine hepatocytes. Experiment 1: Liver samples were obtained immediately post-mortem from one healthy Angus steer. Read More

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Graphical Representation of the Movements of Motives of Consciousness in Leontyev's Activity Theory.

Integr Psychol Behav Sci 2021 Jul 31. Epub 2021 Jul 31.

School of Social Theology and Christian Culture, Faculty of Theology, Aristotle University of Thessaloniki, 54124, Thessaloniki, Hellas, Greece.

The present study aims at giving a graphical representation of the movements of motives of consciousness in Leontyev's Activity Theory. In addition, related works concerning "vertical" and "horizontal" in the area of cultural historical activity theory are presented along with demonstrating Leontyev's conceptual distinctions, in a multimodal way, including pastoral psychology and technology. Finally, the last part suggests answers to Vygotsky's and Leontyev's fundamental questions. Read More

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Study on ecological adaptability construction characteristics of residential buildings in Kangba area, Tibet, China.

Environ Sci Pollut Res Int 2021 Jul 31. Epub 2021 Jul 31.

College of Enviromental Science and Engineering, Ocean University of China, Qingdao, 266100, China.

Located in the southwest of the Qinghai-Tibet Plateau, Tibet is characterized by high cold, high radiation, and large differences in temperature between day and night. Tibetan residential buildings are famous for adapting to the harsh ecological environment and maintaining durability. Based on the residential buildings in Tibet, this paper extracts the technical process and color decoration culture in the construction process in order to adapt to the harsh natural environment. Read More

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Image-based cell subpopulation identification through automated cell tracking, principal component analysis, and partitioning around medoids clustering.

Med Biol Eng Comput 2021 Jul 31. Epub 2021 Jul 31.

Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.

In vitro cell culture model systems often employ monocultures, despite the fact that cells generally exist in a diverse, heterogeneous microenvironment in vivo. In response, heterogeneous cultures are increasingly being used to study how cell phenotypes interact. However, the ability to accurately identify and characterize distinct phenotypic subpopulations within heterogeneous systems remains a major challenge. Read More

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Predictors of Latent Class Trajectories of Internalizing Symptoms in Latinx Adolescents.

Res Child Adolesc Psychopathol 2021 Jul 31. Epub 2021 Jul 31.

Department of Psychology, University of Georgia, Athens, GA, USA.

Guided by ecodevelopmental theories, the present study examined how both culture-specific and general risk and protective factors across contexts predicted trajectories of Latinx youth's internalizing symptoms during early and middle adolescence. Participants included 547 Latinx youth (M age = 12.80; 55% females) recruited in middle school and followed prospectively across four time points spanning two years. Read More

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Post-arthrogram synovitis: MRI and histopathologic findings.

Skeletal Radiol 2021 Jul 31. Epub 2021 Jul 31.

University of Missouri at Columbia, Columbia, MO, USA.

A 57-year-old patient developed severe, persistent pain following MR arthrography with iodinated contrast. MRI 1 week later showed synovitis which was new compared to the prior MRI. Arthroscopy showed severe synovitis. Read More

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