1,195,189 results match your criteria cultures


A liquid-culture-based screening approach to study compounds affecting inflammatory processes in .

Biol Chem 2021 Sep 20. Epub 2021 Sep 20.

Department of Psychiatry and Psychotherapy, University Medical Centre of the Johannes Gutenberg-University Mainz, D-55131Mainz, Germany.

Discovery of biomedical drugs makes use of novel biological sources of limited availability and is often in need of fast, small-scale initial screening approaches. Here, we present a screening, based on the reporter strain IG692, for identification of anti- and pro-inflammatory properties. The elaborated workflow is based on cultivation in fluid and by this, allows fast and reproducible seeding in 96 well plates. Read More

View Article and Full-Text PDF
September 2021

"The Story I will Never Forget": Critical Incident Narratives in Surgical Residency.

Ann Surg 2021 Sep 16. Epub 2021 Sep 16.

Michigan State University College of Human Medicine Michigan Medicine, Department of Surgery Beaumont Health, Department of Surgery Yale University, Department of Surgery.

Objective: We sought to better understand what defines a critical incident experience for the surgical trainee.

Summary Background Data: Critical incidents are formative moments stamped indelibly on one's memory that shape professional identity. The critical incident technique - using participants' narratives to identify patterns and learn from their perceptions - has been explored in some healthcare settings, but there has been no inquiry within surgery. Read More

View Article and Full-Text PDF
September 2021

Aptamer functionalized DNA hydrogels: Design, applications and kinetics.

Biosens Bioelectron 2021 Sep 3;194:113597. Epub 2021 Sep 3.

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China. Electronic address:

DNA hydrogels have received considerable attention in various promising applications due to their excellent biocompatibility, controlled biodegradability, adjustable mechanical properties, stability against proteases, self-healing ability, and stimuli responsiveness. To obtain the specific molecular recognition capability, aptamers and many other functional motifs are utilized. Aptamers are short single-stranded DNA or RNA selected through SELEX to bind with specific target with high affinity and specificity. Read More

View Article and Full-Text PDF
September 2021

In vitro cultivation of Trypanosoma congolense bloodstream forms: State of the art and advances.

Vet Parasitol 2021 Sep 9;299:109567. Epub 2021 Sep 9.

CIRAD, UMR INTERTRYP, F-34398, Montpellier, France; INTERTRYP, Univ Montpellier, CIRAD, IRD, Montpellier, France.

Animal African Trypanosomosis (AAT or Nagana) is a severe vector-borne disease caused by protozoan parasites belonging to the Trypanosomatidae family and is usually cyclically transmitted by blood-sucking tsetse flies. AAT remains a major problem in sub-Saharan Africa. Among the main AAT causative agents, Trypanosoma congolense (T. Read More

View Article and Full-Text PDF
September 2021

Microrna analysis of human decidua mesenchymal stromal cells from preeclampsia patients.

Placenta 2021 Sep 10;115:12-19. Epub 2021 Sep 10.

Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, Istanbul, Turkey. Electronic address:

Introduction: In preeclampsia (PE), human decidua mesenchymal stromal cells (hDMSCs) are exposed to abnormally high levels of oxidative stress and inflammatory factors circulating in the maternal blood. MicroRNAs (miRNAs) have been shown to have a significant impact on the differentiation, maturation and function of mesenchymal stromal cells (MSCs). Our aim in the present study is firstly to investigate differentially expressed miRNA levels to be used as a biomarker in the early detection of PE and secondly to investigate whether those differentially expressed miRNAs in hDMSCs have an effect on the pathogenesis of PE. Read More

View Article and Full-Text PDF
September 2021

Uninformative and unuseful: why it is necessary to actively challenge COVID-19 antibody testing postvaccination.

Public Health 2021 Aug 25;199:32-33. Epub 2021 Aug 25.

Fundación Octaedro, Quito, Ecuador.

Objectives: We aimed to assess the evidence on the usefulness of postvaccination testing of COVID-19 antibodies.

Study Design: We used a descriptive analytical approach.

Methods: We synthesized insights of studies on the immunological responses to SARS-CoV-2 after natural infection or vaccination and recommendations by regulatory institutions, such as the Food and Drug Administration and the Centers for Disease Control and Prevention in the United States. Read More

View Article and Full-Text PDF

"Maybe what happened is actually OK?" Child sexual abuse by authority figures in the ultra-orthodox community in Israel: Survivors' perceptions of their relationships with the perpetrator.

Child Abuse Negl 2021 Sep 14;122:105325. Epub 2021 Sep 14.

Paul Baerwald School of Social Work and Social Welfare, Hebrew University of Jerusalem, 91905, Israel. Electronic address:

Background: Child sexual abuse (CSA) by authority figures in a religious community (AFRCs) has been studied extensively among the Catholic clergy, and to a limited extent among Orthodox Jewish communities in the United States and Australia. However, less attention has been devoted to the phenomenon within the Israeli context.

Objective: This article examines the perceptions of survivors within the ultra-Orthodox Jewish community in Israel of their relationships with authority figures in the community who sexually abused them. Read More

View Article and Full-Text PDF
September 2021

Disability in a medieval village community: A unique case of facial dysmorphism.

Int J Paleopathol 2021 Sep 14;35:22-28. Epub 2021 Sep 14.

UMR 7269 LAMPEA, Aix Marseille Univ, CNRS, Minist Culture, France.

Objective: To identify the pathology causing the severe facial dysmorphia of a medieval individual from the site of Rigny (Indre-et-Loire, France) and to evaluate its functional repercussions on the subject's hearing and social life.

Materials: An individual from Rigny for the osteological study, 69 individuals from the region for the Ct-Scan study and 48 individuals from the site for the isotopic analysis.

Methods: Macroscopic analysis was performed using standard osteological methods. Read More

View Article and Full-Text PDF
September 2021

Hybrid biofabrication of 3D osteoconductive constructs comprising Mg-based nanocomposites and cell-laden bioinks for bone repair.

Bone 2021 Sep 14:116198. Epub 2021 Sep 14.

Christchurch Regenerative Medicine and Tissue Engineering (CReaTE) Group, Department of Orthopaedics Surgery and Musculoskeletal Medicine, University of Otago Christchurch, Christchurch, New Zealand; Centre of Research Excellence in Medical Technologies (MedTech CoRE), Auckland, New Zealand. Electronic address:

Tissue engineering approaches for bone repair have rapidly evolved due to the development of novel biofabrication technologies, providing an opportunity to fabricate anatomically-accurate living implants with precise placement of specific cell types. However, limited availability of biomaterial inks, that can be 3D-printed with high resolution, while providing high structural support and the potential to direct cell differentiation and maturation towards the osteogenic phenotype, remains an ongoing challenge. Aiming towards a multifunctional biomaterial ink with high physical stability and biological functionality, this work describes the development of a nanocomposite biomaterial ink (Mg-PCL) comprising of magnesium hydroxide nanoparticles (Mg) and polycaprolactone (PCL) thermoplastic for 3D printing of strong and bioactive bone regenerative scaffolds. Read More

View Article and Full-Text PDF
September 2021

Radioresistance and transcriptional reprograming of invasive glioblastoma cells.

Int J Radiat Oncol Biol Phys 2021 Sep 14. Epub 2021 Sep 14.

Division of Molecular & Translational Radiation Oncology, Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg University Hospital (UKHD), Heidelberg, Germany; Cancer Consortium (DKTK) Core-Center, German Cancer Research Center (DKFZ), Heidelberg, Germany; Heidelberg Institute of Radiation Oncology (HIRO), National Center for Radiation Oncology (NCRO), German Cancer Research Center (DKFZ), Heidelberg University Hospital (UKHD), Faculty of Medicine (MFHD) of the Heidelberg University, and Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany; CCU Translational Radiation Oncology, CCU Radiation Oncology, CCU Neurooncology, National Center for Tumor Diseases (NCT) German Cancer Research Center (DKFZ), Heidelberg University Hospital (UKHD), Heidelberg, Germany; Departments of Neurology, Neurosurgery and Radiation Oncology, Heidelberg University Hospital (UKHD), Heidelberg, Germany. Electronic address:

Purpose: Infiltrative growth pattern is a hallmark of glioblastoma (GBM). Radiotherapy aims to eradicate microscopic residual GBM cells post-surgical removal of the visible tumor bulk. However, in field recurrences remain the major pattern of therapy failure. Read More

View Article and Full-Text PDF
September 2021

Aerosol generating dental procedures: A reappraisal of analysis methods and infection control measures.

J Hosp Infect 2021 Sep 14. Epub 2021 Sep 14.

Faculty of Dentistry, National University of Singapore, Singapore. Electronic address:

Background: Dental aerosol generating procedures (AGPs) have been associated with risk for transmitting infectious agents. However, existing infection control monitoring studies potentially underestimate the extent of contamination, due to methodological inadequacies. These studies employed settle plate methodology which only captures droplets that land on agar plates, but not those suspended in air. Read More

View Article and Full-Text PDF
September 2021

A Fully Integrated Online Platform for Real Time Monitoring of Multiple Product Quality Attributes in Biopharmaceutical Processes for Monoclonal Antibody Therapeutics.

J Pharm Sci 2021 Sep 14. Epub 2021 Sep 14.

CMC Analytical, Product Development & Supply, GlaxoSmithKline, Pennsylvania 19406, United States.

In response to FDA's call for Quality by Design (QbD) in biopharmaceutical product development, the biopharmaceutical industry has been developing highly sensitive and specific technologies in the monitoring and controlling of product quality attributes for bioprocesses. We previously published the successful application of an off-line multi-attribute method (MAM) to monitor more than 20 critical quality attributes (CQA) with superior sensitivity for the upstream process. To further remove the hurdles of laborious process sampling and sample preparation associated with the offline method, we present here a fully integrated MAM based online platform for automated real time online process monitoring. Read More

View Article and Full-Text PDF
September 2021

14-year trends and resistance patterns of blood and cerebrospinal fluid cultures in children under three years old.

J Infect 2021 Sep 14. Epub 2021 Sep 14.

Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom; Oxford Vaccine Group, Department of Paediatrics, University of Oxford, United Kingdom; NIHR Oxford Biomedical Research Centre, Level 2, Children's Hospital, Oxford, United Kingdom; Paediatric Infectious Diseases Research Group, Institute of Infection and Immunity, St George's, University of London, London, United Kingdom. Electronic address:

Objectives: Bacterial infections are a major cause of UK paediatric hospitalisations, yet longitudinal data on causative organisms or antimicrobial resistance are scarce. This retrospective analysis describes trends in blood and cerebrospinal fluid (CSF) cultures and resistance patterns in children under three years old from a large UK centre.

Methods: All culture results, and resistance data for Gram-negative rods (GNR) in blood cultures, collected between January 2005 and December 2018 were extracted from Oxford University Hospitals NHS Foundation Trust microbiology database. Read More

View Article and Full-Text PDF
September 2021

Kidney toxicity of the BRAF-kinase inhibitor vemurafenib is driven by off-target ferrochelatase inhibition.

Kidney Int 2021 Sep 14. Epub 2021 Sep 14.

Division of Pharmaceutics and Pharmacology, College of Pharmacy & Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA. Electronic address:

A multitude of disease and therapy related factors drive the frequent development of kidney disorders in cancer patients. Along with chemotherapy, the newer targeted therapeutics can also cause kidney dysfunction through on and off-target mechanisms. Interestingly, among the small molecule inhibitors approved for the treatment of cancers that harbor BRAF-kinase activating mutations, vemurafenib can trigger tubular damage and acute kidney injury. Read More

View Article and Full-Text PDF
September 2021

Testicular 25-hydroxycholesterol: An alternate substrate for steroidogenesis in reptiles.

Gen Comp Endocrinol 2021 Sep 14:113906. Epub 2021 Sep 14.

Department of Zoology, University of Delhi, Delhi 110007, India. Electronic address:

The current study in wall lizards Hemidactylus flaviviridis was designed to ascertain that Leydig cells utilize testicular macrophage-derived 25-hydroxycholesterol (25-HC) for steroidogenesis. Leydig cells (LC) collected from regressed testes when incubated with 25-HC that was obtained from HPLC-eluted fraction of testicular macrophage-conditioned medium (TMCM), lyophilized and reconstituted in culture medium (0.5 µg/ml/well), produced considerably higher amount of testosterone. Read More

View Article and Full-Text PDF
September 2021

In vitro efficacy of different concentrations of Duddingtonia flagrans on varying egg densities of gastrointestinal nematodes of cattle.

Exp Parasitol 2021 Sep 14:108156. Epub 2021 Sep 14.

Área de Parasitología y Enfermedades Parasitarias, Centro de Investigación Veterinaria de Tandil (CIVETAN) (CONICET-UNCPBA-CICPBA), Facultad de Ciencias Veterinarias, UNCPBA, Tandil, Argentina.

The nematophagous fungus Duddingtonia flagrans, used for the biological control of gastrointestinal nematodes in livestock, is fed to infected animals so its chlamydospores and the parasite eggs are voided together with faeces where the fungus preys on nematode larvae, thus reducing pasture infectivity. The number of chlamydospores needed for the fungus to be efficient in the presence of a wide range in numbers of parasitic eggs is largely unknown and a matter of discussion. The aim of this study was to determine the fungal efficacy of four different chlamydospore concentrations against three different levels of cattle faecal egg counts. Read More

View Article and Full-Text PDF
September 2021

Baby-led weaning: Health professionals 'knowledge and attitudes and parents' experiences from Spain. A mixed methods approach.

Health Soc Care Community 2021 Sep 17. Epub 2021 Sep 17.

SALBIS Research Group, Faculty of Health Sciences, Ponferrada Campus, Universidad de León, Leon, Spain.

Baby-led weaning or self-regulated small chunk feeding by the baby has emerged as an alternative to the traditional method of complementary feeding. Parents and healthcare professionals often differ in the benefits and drawbacks about it. The research aims were to describe the knowledge of health professionals about complementary feeding and baby-led weaning (BLW) method and the attitudes towards its recommendation, and to explore the knowledge, experiences and attitudes of parents who have used this method to feed their children. Read More

View Article and Full-Text PDF
September 2021

RAS induced senescence of skin keratinocytes is mediated through Rho-associated protein kinase (ROCK).

Mol Carcinog 2021 Sep 17. Epub 2021 Sep 17.

Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA.

Cellular senescence is a well-documented response to oncogene activation in many tissues. Multiple pathways are invoked to achieve senescence indicating its importance to counteract the transforming activities of oncogenic stimulation. We now report that the Rho-associated protein kinase (ROCK) signaling pathway is a critical regulator of oncogene-induced senescence in skin carcinogenesis. Read More

View Article and Full-Text PDF
September 2021

Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.

Brain 2021 Sep 17. Epub 2021 Sep 17.

Department of Biotechnology, Jožef Stefan Institute, Ljubljana, Slovenia.

Repeat expansions in the C9orf72 gene are a common cause of amyotrophic lateral sclerosis and frontotemporal lobar degeneration, two devastating neurodegenerative disorders. One of the proposed mechanisms of GGGGCC repeat expansion is their translation into non-canonical dipeptide repeats, which can then accumulate as aggregates and contribute to these pathologies. There are five different dipeptide repeat proteins (polyGA, polyGR, polyPR, polyPA and polyGP), some of which are known to be neurotoxic. Read More

View Article and Full-Text PDF
September 2021

Isothermal amplification and fluorescent detection of SARS-CoV-2 and SARS-CoV-2 variant virus in nasopharyngeal swabs.

PLoS One 2021 17;16(9):e0257563. Epub 2021 Sep 17.

Department of Infectious Disease, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.

The COVID-19 pandemic caused by the SARS-CoV-2 is a serious health threat causing worldwide morbidity and mortality. Real-time reverse transcription PCR (RT-qPCR) is currently the standard for SARS-CoV-2 detection. Although various nucleic acid-based assays have been developed to aid the detection of SARS-CoV-2 from COVID-19 patient samples, the objective of this study was to develop a diagnostic test that can be completed in 30 minutes without having to isolate RNA from the samples. Read More

View Article and Full-Text PDF
September 2021

Culture of Interdisciplinary Collaboration in Nursing Research Training.

Nurs Res 2021 Sep 15. Epub 2021 Sep 15.

University of Florida Clinical Translational Science Institute, Gainesville, FL.

Background: Establishing and maintaining collaborative scientific environments that can cultivate and benefit from a full range of talents is essential for the quality and influence of science. Inclusion of research training and career development interventions to expose nursing PhD students, postdocs, and junior faculty to team science stands to prepare graduates to effectively engage with interdisciplinary colleagues to conduct cutting-edge nursing research and compete successfully for precious research resources. To be effective, nursing research workforce development programs need to recognize and share a culture of interdisciplinarity. Read More

View Article and Full-Text PDF
September 2021

Provider perceptions of lack of supportive care during childbirth: A mixed methods study in Kenya.

Health Care Women Int 2021 Sep 17:1-22. Epub 2021 Sep 17.

UCSF Institute for Global Health Sciences, San Francisco, California, USA.

Supportive care is a key component of person-centered maternity care (PCMC), and includes aspects such as timely and attentive care, pain control, and the health facility environment. Yet, few researchers have explored the degree of supportive care delivered or providers' perceptions on supportive care practices during childbirth. The researchers' aim is to evaluate the extent of supportive care provided to women during childbirth and to identify the drivers behind the lack of supportive care from the perspective of maternity providers in a rural county in Western Kenya. Read More

View Article and Full-Text PDF
September 2021

The transition to university in a sample of Italian students: the role of integrative memories of high school transition.

Memory 2021 Sep 17:1-12. Epub 2021 Sep 17.

Department of Psychology, University of Turin, Turin, Italy.

Grounded in literature on school transitions and autobiographical memory, this study focuses on the personal memories of a sample of 112 young Italian adults facing their transition to university. Participants were asked to recall five memories of their high school transition experience and, subsequently, the students were assessed in terms of resilience, future orientation, academic motivation, university transition experience, and subjective well-being since attending university. The memories were coded for narrative themes, structure, and integrative meaning. Read More

View Article and Full-Text PDF
September 2021

Thin Thermoresponsive Polymer Films for Cell Culture: Elucidating an Unexpected Thermal Phase Behavior by Atomic Force Microscopy.

Langmuir 2021 Sep 17. Epub 2021 Sep 17.

Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya Street, Moscow 119991, Russia.

Application of poly--isopropylacrylamide (PNIPAM) and its more hydrophobic copolymers with --butylacrylamide (NtBA) as supports for cell sheets has been validated in numerous studies. The binary systems of these polymers with water are characterized by a lower critical solution temperature (LCST) in a physiologically favorable region. Upon lowering the temperature below the LCST, PNIPAM chains undergo a globule-to-coil transition, causing the film dissolution and cell sheet detachment. Read More

View Article and Full-Text PDF
September 2021

CTLA-4 polymorphisms in Thai patients with rheumatoid arthritis, systemic lupus erythematosus, and systemic sclerosis.

Int J Rheum Dis 2021 Sep 17. Epub 2021 Sep 17.

School of Pharmacological Sciences, University of Shizuoka, Shizuoka, Japan.

Aims: Studies on polymorphisms of the cytotoxic T lymphocytes associated antigen-4 (CTLA-4) genes in rheumatic disease patients are limited in Southeast Asia. This pilot study aimed to determine CTLA-4 polymorphisms in Thai patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc), and correlate them with serology.

Method: One-hundred RA, 70 SLE and 50 SSc patients, and 99 healthy controls (HCs) were included in this study. Read More

View Article and Full-Text PDF
September 2021

Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation.

FASEB J 2021 Oct;35(10):e21926

Department of Animal Sciences and D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville, Florida, USA.

The preimplantation period of embryonic development can be a key window for programming of postnatal development because extensive epigenetic remodeling occurs during this time. It was hypothesized that modification of one-carbon metabolism of the bovine embryo by addition of the methyl-donor choline to culture medium would change postnatal phenotype through epigenetic modification. Embryos produced in vitro were cultured with 1. Read More

View Article and Full-Text PDF
October 2021

Drug-Induced Liver Injury: Highlights and Controversies in the Recent Literature.

Drug Saf 2021 Sep 17. Epub 2021 Sep 17.

Division of Gastroenterology and Hepatology, Medstar Georgetown University Hospital, Washington, DC, USA.

Drug-induced liver injury (DILI) remains an important, yet challenging diagnosis for physicians. Each year, additional drugs are implicated in DILI and this year was no different, with more than 1400 articles published on the subject. This review examines some of the most significant highlights and controversies in DILI-related research over the past year and their implications for clinical practice. Read More

View Article and Full-Text PDF
September 2021

Immediate Glucose-Lowering Effect After the First Administration of Dulaglutide: A Retrospective, Single-Center, Observational Study.

Diabetes Ther 2021 Sep 17. Epub 2021 Sep 17.

Department of Endocrinology and Metabolism, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.

Introduction: Dulaglutide is a long-acting glucagon-like peptide 1 receptor agonist that is administered once weekly for the treatment of type 2 diabetes. However, the immediate glucose-lowering effect of dulaglutide after the first administration and the factors affecting the efficacy of the drug remain unclear.

Methods: This study was a retrospective and observational study of 80 subjects with type 2 diabetes conducted in a hospitalized setting. Read More

View Article and Full-Text PDF
September 2021

The learning experiences of dyslexic medical students during the COVID-19 pandemic: a phenomenological study.

Adv Health Sci Educ Theory Pract 2021 Sep 17. Epub 2021 Sep 17.

Department of Medical Education, Brighton and Sussex Medical School, Brighton, East Sussex, UK.

Dyslexia is a Specific Learning Difficulty that impacts on reading and writing abilities. During the COVID-19 pandemic, medical schools have been forced to undertake distance learning and assessment. The wider literature suggested that e-learning might pose additional challenges for dyslexic students. Read More

View Article and Full-Text PDF
September 2021

Knockdown of Dnmt1 and Dnmt3a gene expression disrupts preimplantation embryo development through global DNA methylation.

J Assist Reprod Genet 2021 Sep 17. Epub 2021 Sep 17.

Laboratory for Stem Cells and Reproductive Cell Biology, Department of Histology and Embryology, Ankara University School of Medicine, Ankara, Turkey.

Purpose: DNA methylation is one of the epigenetic mechanisms that plays critical roles in preimplantation embryo development executed by DNA methyltransferase (Dnmt) enzymes. Dnmt1, responsible for the maintenance of methylation, and Dnmt3a, for de novo methylation, are gradually erased from the zygote in succeeding stages and then reestablished in the blastocyst. This study was designed to address the vital role of Dnmt1 and Dnmt3a enzymes by silencing their gene expressions in embryonic development in mice. Read More

View Article and Full-Text PDF
September 2021