278,423 results match your criteria culture medium

Distinct biofilm formation regulated by different culture media: Implications to electricity generation.

Bioelectrochemistry 2021 Apr 27;140:107826. Epub 2021 Apr 27.

Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China. Electronic address:

Biofilm of Shewanella oneidensis MR-1 is extensively studied as it can transform organic compounds directly into electricity. Although revealing the biofilm regulation mechanism is crucial for enhancing bio-current, studies regarding the mechanism by which the culture condition affects biofilm formation are still lacking. The biofilm formation of S. Read More

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Expression profile of adhesion molecules in blastocyst vis-a-vis uterine epithelial cells.

Theriogenology 2021 May 5;170:36-45. Epub 2021 May 5.

Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, India.

Models using in vitro produced buffalo embryos and in vitro cultured uterine epithelial cells (UECs) may be useful in understanding the intricacies of embryo-uterine cross talk. In the present study, buffalo UECs were obtained from slaughterhouse derived non-gravid uterus. UECs monolayer was treated with steroids (10pg/ml estradiol for 24h and 3. Read More

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Canine mast cell tumour cells regulate tryptophan catabolism via the expression of indoleamine 2,3-dioxygenase.

Res Vet Sci 2021 Apr 30;137:159-162. Epub 2021 Apr 30.

Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.

Indoleamine 2,3-deoxygenase (IDO) produced by cancer cells catabolizes tryptophan (TRP) to kynurenine (KYN) in the environment, resulting induction of cancer immune escape through induction of T cell anergy and enhancement of regulatory T cells. Recently, inhibition of IDO has been recognized as one of therapeutic strategies for human neoplastic diseases. However, there have been few reports about IDO-expressing cancers in dogs. Read More

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IL-18 promotes erythrophagocytosis and erythrocyte degradation by M1 macrophages in a calcific microenvironment: IL-18 promotes erythrophagocytosis and degradation.

Can J Cardiol 2021 May 10. Epub 2021 May 10.

Department of Cardiovascular Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China. Shanghai Chest Hospital, Huaihai Road, Shanghai, China, 200000.. Electronic address:

Background: Calcific aortic valve disease (CAVD) is the most common cause of aortic valve replacement in developed countries. Intraleaflet haemorrhage has been found to be positively correlated with the progression of CAVD. Although most research has focused on erythrocyte degradation products, which promote CAVD progression, the process of erythrophagocytosis and erythrocyte degradation by macrophages in intraleaflet haemorrhage areas has remained unexplored. Read More

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Effects of human platelet lysate on the growth of cultured human corneal endothelial cells.

Exp Eye Res 2021 May 10:108613. Epub 2021 May 10.

Save Sight Institute, The University of Sydney, Sydney, NSW, 2000, Australia.

Human platelet lysate (hPL) as a replacement for foetal bovine serum (FBS) in culturing human corneal endothelium is an emerging area of interest, although there are limited studies evaluating the quality of the hPL being used. Our study aimed to evaluate variations between sources of hPL and to explore the efficacy of hPL (with and without heparin) as a replacement for FBS in culturing human corneal endothelial cells in vitro. Immortalized human corneal endothelial cells (B4G12) and primary human corneal endothelial cells (PHCEnCs, n = 11 donors, age from 36-85 years old) were cultured with 5% hPL or FBS. Read More

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A new mechanism of obeticholic acid on NASH treatment by inhibiting NLRP3 inflammasome activation in macrophage.

Metabolism 2021 May 10:154797. Epub 2021 May 10.

Laboratory of Anti-inflammation, Shanghai, Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, PR China. Electronic address:

Objective: Obeticholic acid (OCA) has been proved to play potential therapeutic effect on nonalcoholic steatohepatitis (NASH). Up to now, the study of OCA on NLRP3 inflammasome activation in macrophage is still blank and merits great attention. Here, we aimed to better characterize the role and mechanism of OCA on NASH treatment focusing on NLRP3 inflammasome activation in macrophages. Read More

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Neuronal circuits on a chip for biological network monitoring.

Biotechnol J 2021 May 13:e2000355. Epub 2021 May 13.

Group of Optics, Photonics and Biophotonics (GOFB), Center for Biomedical Technology, Universidad Politécnica de Madrid, 28223 Pozuelo de Alarcón, Madrid, Spain.

Cultured neuronal networks (CNNs) are a robust model to closely investigate neuronal circuits' formation and monitor their structural properties evolution. Typically, neurons are cultured in plastic plates or, more recently, in microfluidic platforms with potentially a wide variety of neuroscience applications. As a biological protocol, cell culture integration with a microfluidic system provides benefits such as accurate control of cell seeding area, culture medium renewal, or lower exposure to contamination. Read More

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Anti-Semitism in the United States: An Overview and Strategies to Create a More Socially Just Society.

Soc Work 2021 May;66(2):128-138

Hate crimes against members of the Jewish community have increased dramatically over the past few years. According to federal data, the number of hate crimes directed at Jews now appears to exceed those directed at many, if not most, minority groups. Yet, despite the number of hate crimes aimed at Jews, little recent scholarship has considered the issue of anti-Semitism. Read More

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The Impact of COVID-19 on Conjunctival Flora.

Ocul Immunol Inflamm 2021 May 13:1-6. Epub 2021 May 13.

Department of Infectious Diseases and Clinical Microbiology, Malatya Training and Research Hospital, Malatya, Turkey.

: This study aims to evaluate the effect of coronavirus disease 2019 (COVID-19) on conjunctival flora in patients hospitalized with COVID-19.: This prospective, controlled study was conducted between June 2020 and December 2020. The study group consisted of 45 confirmed COVID-19 patients and 43 control subjects. Read More

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Influences and mechanisms of nanoparticles on pentachloronitrobenzene accumulation by earthworms.

Environ Sci Pollut Res Int 2021 May 13. Epub 2021 May 13.

Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China.

Pesticides and nanoparticles may coexist in soil; however, influences of nanoparticles on accumulation of pesticides in terrestrial organisms are still unclear. This study aims to investigate the influences and mechanisms of metal oxide nanoparticles (nano ZnO and nano CuO) on accumulation of pentachloronitrobenzene (PCNB) in earthworms and their combined toxicity. The earthworms were cultivated in the soil spiked with nanoparticles (10, 50, 250 mg/kg) and PCNB (100 μg/kg) for 21 days. Read More

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Understanding the differences in 2G ethanol fermentative scales through omics data integration.

FEMS Yeast Res 2021 May 13. Epub 2021 May 13.

Department of Genetics, Evolution, Microbiology, and Immunology, Institute of Biology, University of Campinas, Campinas, SP, 13083-862, Brazil.

In this work, we evaluated the fermentative performance and metabolism modifications of a second generation (2G) industrial yeast by comparing an industrial condition during laboratory and industrial scale fermentations. Fermentations were done using industrial lignocellulosic hydrolysate and a synthetic medium containing inhibitors and analyses were carried out through transcriptomics and proteomics of these experimental conditions. We found that fermentation profiles were very similar, but there was an increase in xylose consumption rate during fermentations using synthetic medium when compared to lignocellulosic hydrolysate, likely due to the presence of unknown growth inhibitors contained in the hydrolysate. Read More

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Analysis of Cyberincivility in Posts by Health Professions Students: Descriptive Twitter Data Mining Study.

JMIR Med Educ 2021 May 13;7(2):e28805. Epub 2021 May 13.

College of Nursing, Ewha Womans University, Seoul, Republic of Korea.

Background: Health professions students use social media to communicate with other students and health professionals, discuss career plans or coursework, and share the results of research projects or new information. These platforms allow students to share thoughts and perceptions that are not disclosed in formal education settings. Twitter provides an excellent window through which health professions educators can observe students' sociocultural and learning needs. Read More

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[Research strategies for endophytes in medicinal plants based on high-throughput sequencing and traditional culture and isolation methods].

Zhongguo Zhong Yao Za Zhi 2021 Apr;46(8):1910-1919

State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

The research on endophytes of medicinal plants mainly relies on the traditional culture and isolation methods. Because of their functions such as promoting host growth, improving stress resistance, promoting the accumulation of medicinal active ingredients or directly producing medicinal active ingredients, the endophytes of medicinal plants have gradually attracted wide attention. However, it was found that the strains isolated by traditional methods were not the true dominant endophytes of medicinal plants by comparing the results of traditional culture isolation with high-throughput sequencing. Read More

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A harmonized and standardized in vitro approach produces reliable results on silver nanoparticles toxicity in different cell lines.

J Appl Toxicol 2021 May 12. Epub 2021 May 12.

Department of Environment and Health, Istituto Superiore di Sanità, Rome, Italy.

Despite the widespread use of silver nanoparticles (AgNPs) in different fields and the amount of investigations available, to date, there are many contradictory results on their potential toxicity. In the present study, extensively characterized 20-nm AgNPs were investigated using optimized protocols and standardized methods to test several toxicological endpoints in different cell lines. The agglomeration/aggregation state of AgNPs in culture media was measured by dynamic light scattering (DLS). Read More

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Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos.

Biol Reprod 2021 May 12. Epub 2021 May 12.

School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.

The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. Read More

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Marine microalgae co-cultured with floc-forming bacterium: Insight into growth and lipid productivity.

PeerJ 2021 23;9:e11217. Epub 2021 Apr 23.

Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.

This study investigated the effect of co-culturing microalgae with a floc-forming bacterium. Of the six microalgae isolated from a biofloc sample, only , sp. and were propagated successfully in Conway medium. Read More

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Bone Mesenchymal Stem Cells Contribute to Ligament Regeneration and Graft-Bone Healing after Anterior Cruciate Ligament Reconstruction with Silk-Collagen Scaffold.

Stem Cells Int 2021 23;2021:6697969. Epub 2021 Apr 23.

Department of Orthopedic Surgery, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, China 450001.

Anterior cruciate ligament (ACL) reconstruction was realized using a combination of bone mesenchymal stem cells (BMSCs) and silk-collagen scaffold, and an in vivo evaluation of this combination was performed. By combining type I collagen and degummed silk fibroin mesh, silk-collagen scaffolds were prepared to simulate ligament components. BMSCs isolated from bone marrow of rabbits were cultured for a homogenous population and seeded on the silk-collagen scaffold. Read More

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Optimization of PhysicoChemical Parameters for Production of Cytotoxic Secondary Metabolites and Apoptosis Induction Activities in the Culture Extract of a Marine Algal-Derived Endophytic Fungus sp.

Front Pharmacol 2021 26;12:542891. Epub 2021 Apr 26.

Department of Biochemistry, Indian Institute of Science, Bangalore, India.

The endophytic fungal community in the marine ecosystem has been demonstrated to be relevant source of novel and pharmacologically active secondary metabolites. The current study focused on the evaluation of cytotoxic and apoptosis induction potential in the culture extracts of endophytic fungi associated with , a marine brown alga. The cytotoxicity of the four marine endophytes, sp. Read More

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The anesthetic sevoflurane induces tau trafficking from neurons to microglia.

Commun Biol 2021 May 12;4(1):560. Epub 2021 May 12.

Geriatric Anesthesia Research Unit, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA.

Accumulation and spread of tau in Alzheimer's disease and other tauopathies occur in a prion-like manner. However, the mechanisms and downstream consequences of tau trafficking remain largely unknown. We hypothesized that tau traffics from neurons to microglia via extracellular vesicles (EVs), leading to IL-6 generation and cognitive impairment. Read More

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Improved selective BIN agar for a better rate of isolation from primary clinical specimens in suspected Madagascar's plague cases.

J Clin Microbiol 2021 May 12. Epub 2021 May 12.

Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona, Israel.

According to the WHO, 75% of the world's plague cases are found in Madagascar, with an average of 200-700 suspected cases annually (mainly bubonic plague). In 2017, a pneumonic plague epidemic of unusual proportions occurred, which raised several challenges for laboratory confirmation of cases, pointing to the need for the development of isolation procedures, especially those that can be performed in remote areas. As the WHO gold standard for plague diagnosis is bacterial culture, we sought to develop a simple method to prepare a highly selective medium, fit for use in remote endemic areas. Read More

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Determination of optimal culture conditions for toxin production by a Prorocentrum lima complex strain with high diarrhetic shellfish toxins yield.

Harmful Algae 2021 Mar 5;103:102025. Epub 2021 Apr 5.

Laboratory of Aquatic Environmental Science (LAQUES), Faculty of Agriculture and Marine Science, Kochi University, 200 Otsu, Monobe, Nankoku, Kochi 783-8502, Japan. Electronic address:

Diarrhetic shellfish poisoning (DSP) is caused by the consumption of shellfish contaminated by diarrhetic shellfish toxins (DSTs) such as okadaic acid (OA) and dinophysistoxins (DTXs) produced by some species of dinoflagellates. To prevent the occurrence of human intoxication cases, inspection of DSTs (OA and DTXs) in shellfish is important. An instrumental method using liquid chromatography-tandem mass spectrometry (LC/MS/MS) has been recently employed in Japan for the monitoring of OA and DTXs in shellfish. Read More

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The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytes.

Pediatr Rheumatol Online J 2021 May 12;19(1):72. Epub 2021 May 12.

Division of Rheumatology, Nemours/Alfred I. duPont Hospital for Children, 1701 Rockland Rd, Wilmington, DE, 19803, USA.

Background: We examined influences of conditioned media from chondrocytes (Ch) on juvenile idiopathic arthritis synovial fibroblasts (JFLS) and potential for JFLS to undergo endochondral bone formation (EBF).

Methods: Primary cells from three control fibroblast-like synoviocytes (CFLS) and three JFLS were cultured in Ch-conditioned media and compared with untreated fibroblast-like synoviocytes (FLS). RNA was analyzed by ClariomS microarray. Read More

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Production of IgY polyclonal antibody against diphtheria toxin and evaluation of its neutralization effect by Vero cell assay.

BMC Biotechnol 2021 May 12;21(1):34. Epub 2021 May 12.

Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Poursina Street, Keshavarz Boulevard, Tehran, Iran.

Background: Diphtheria is a bacterial disease which is caused by Corynebacterium diphtheriae. The symptoms are due to the diphtheria toxin produced by the bacteria. Antibiotic therapy and the use of diphtheria antitoxin is a recommended strategy to control diphtheria. Read More

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Co-culturing microbial consortia: approaches for applications in biomanufacturing and bioprocessing.

Crit Rev Biotechnol 2021 May 12:1-27. Epub 2021 May 12.

Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield, UK.

The application of microbial co-cultures is now recognized in the fields of biotechnology, ecology, and medicine. Understanding the biological interactions that govern the association of microorganisms would shape the way in which artificial/synthetic co-cultures or consortia are developed. The ability to accurately predict and control cell-to-cell interactions fully would be a significant enabler in synthetic biology. Read More

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Cultured human uveal melanocytes express/secrete CXCL1 and CXCL2 constitutively and increased by lipopolysaccharide via activation of Toll-like receptor 4.

Curr Eye Res 2021 May 12. Epub 2021 May 12.

Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Purpose: Lipopolysaccharide (LPS) can activate Toll-like receptor 4 (TLR4) and increase the expression of CXCL1 and CXCL2, the potent neutrophils chemoattractants, in various cell types. These effects have not been previously reported in the uveal melanocytes. This study was designed to investigate the effects of LPS on the activation of TLR4 and expression of CXCL1/CXCL2 in cultured human uveal melanocytes and the relevant signal pathways. Read More

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Enhanced sophorolipid production and effective conversion of waste frying oil using dual lipophilic substrates.

Biosci Biotechnol Biochem 2021 May 12. Epub 2021 May 12.

Biochemical Laboratory, Saraya Co., Ltd., Osaka, Japan.

A highly efficient sophorolipid (SL) fermentation process using dual lipophilic substrates (DLS) was developed. Using DLS consisting of palm oil and oleic acid in the flask fermentation with SL-producing yeast Starmerella bombicola, the productivity and yield of SL improved 2.4- and 1. Read More

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Liquid Biopsy for Colorectal Adenoma: Is the Exosomal miRNA Derived From Organoid a Potential Diagnostic Biomarker?

Clin Transl Gastroenterol 2021 May 12;12(5):e00356. Epub 2021 May 12.

Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan.

Introduction: MicroRNAs (miRNAs) can serve as tumor biomarkers; however, their role in evaluating colorectal adenoma (CRA) is unclear. Recently, the organoid culture system enabled long-term expansion of human colon epithelium. This study aimed to examine the potential of exosomal miRNAs extracted from CRA organoids as biomarkers in the clinical liquid biopsy CRA test. Read More

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Calcium isotope fractionation by osteoblasts and osteoclasts, across endothelial and epithelial cell barriers and with binding to proteins.

Am J Physiol Regul Integr Comp Physiol 2021 May 12. Epub 2021 May 12.

Center for Pediatric and Adolescent Medicine, University Hospital Heidelberg, Germanygrid.5253.1.

Timely and accurate diagnosis of osteoporosis is essential for adequate therapy. Calcium isotope ratio (δCa) determination has been suggested as a sensitive, non-invasive and radiation-free biomarker for the diagnosis of osteoporosis, reflecting bone calcium balance. The quantitative diagnostic is based on the calculation of the δCa difference between blood, urine and bone. Read More

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A novel human arterial wall-on-a-chip to study endothelial inflammation and vascular smooth muscle cell migration in early atherosclerosis.

Lab Chip 2021 May 12. Epub 2021 May 12.

School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, 639798, Singapore. and Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 308232, Singapore.

Mechanistic understanding of atherosclerosis is largely hampered by the lack of a suitable in vitro human arterial model that recapitulates the arterial wall structure, and the interplay between different cell types and the surrounding extracellular matrix (ECM). This work introduces a novel microfluidic endothelial cell (EC)-smooth muscle cell (SMC) 3D co-culture platform that replicates the structural and biological aspects of the human arterial wall for modeling early atherosclerosis. Using a modified surface tension-based ECM patterning method, we established a well-defined intima-media-like structure, and identified an ECM composition (collagen I and Matrigel mixture) that retains the SMCs in a quiescent and aligned state, characteristic of a healthy artery. Read More

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Role of D-alanylation of Streptococcus mutans Lipoteichoic Acid in Interspecies Competitiveness.

Mol Oral Microbiol 2021 May 12. Epub 2021 May 12.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.

Background: The D-alanylation of lipoteichoic acid (LTA) is essential for the physiological metabolism of Streptococcus mutans (S. mutans). This study was designed to investigate the influence of D-alanylation of LTA on interspecies competitiveness of S. Read More

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