61,607 results match your criteria culture effective

A novel organ-chip system emulates three-dimensional architecture of the human epithelia and the mechanical forces acting on it.

Biomaterials 2021 Jun 6;275:120957. Epub 2021 Jun 6.

Emulate Inc., 27 Drydock Avenue, 5th Floor, Boston, MA, 02210, USA. Electronic address:

Successful translation of in vivo experimental data to human patients is an unmet need and a bottleneck in the development of effective therapeutics. Organ-on-Chip technology aims to address this need by leveraging recent significant advancements in microfabrication and biomaterials, which enable modeling of organs and their functionality. These microengineered chips offer researchers the possibility to recreate critical elements of native tissue architecture such as in vivo relevant tissue-tissue interface, air-liquid interface, and mechanical forces, including mechanical stretch and fluidic shear stress, which are crucial to recapitulate tissue level functions. Read More

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Microbial degradation of azo dyes by textile effluent adapted, Enterobacter hormaechei under microaerophilic condition.

Microbiol Res 2021 Jun 9;250:126805. Epub 2021 Jun 9.

Geobiotechnology Laboratory, National College (Autonomous), (Affiliated to Bharathidasan University), Tiruchirapalli, Tamil Nadu, 620001, India; PG and Research Department of Botany, National College (Autonomous), Tiruchirapalli, Tamil Nadu, 620001, India.

Landmark and sustainable eco-friendly dye treatment processes are highly desirous to ameliorate their effect on the environment. The present study investigated the azo dye degradation efficiency of adapted Enterobacter hormaechei SKB 16 from textile effluent polluted soil in optimized culture conditions. The adapted bacteria strain was identified by standard microbiological and molecular techniques. Read More

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Stabilization of fatty acid synthesis enzyme acetyl-CoA carboxylase 1 suppresses acute myeloid leukemia development.

J Clin Invest 2021 Jun;131(12)

Cancer cells reprogram lipid metabolism during their malignant progression, but limited information is currently available on the involvement of alterations in fatty acid synthesis in cancer development. We herein demonstrate that acetyl-CoA carboxylase 1 (ACC1), a rate-limiting enzyme for fatty acid synthesis, plays a critical role in regulating the growth and differentiation of leukemia-initiating cells. The Trib1-COP1 complex is an E3 ubiquitin ligase that targets C/EBPA, a transcription factor regulating myeloid differentiation, for degradation, and its overexpression specifically induces acute myeloid leukemia (AML). Read More

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Compassion in nursing: exploring the perceptions of students and academics.

Nurs Stand 2021 Jun 14. Epub 2021 Jun 14.

School of Health & Social Care, Edinburgh Napier University, Edinburgh, Scotland.

Background: Compassion is integral to effective nursing practice, yet there is limited empirical research exploring this concept, particularly from a professional perspective. Aim To advance understanding of compassion from a professional perspective, specifically through the perceptions of students and academics from the fields of adult, child, learning disability and mental health nursing.

Method: A constructivist grounded theory study was undertaken, and a theoretical sampling strategy was used to guide the selection of appropriate participants. Read More

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Qualities of Effective Vital Anaesthesia Simulation Training Facilitators Delivering Simulation-Based Education in Resource-Limited Settings.

Anesth Analg 2021 Jul;133(1):215-225

Department of Anesthesia, Pain Management, and Perioperative Medicine.

Background: Lack of access to safe and affordable anesthesia and surgical care is a major contributor to avoidable death and disability across the globe. Effective education initiatives are a viable mechanism to address critical skill and process gaps in perioperative teams. Vital Anaesthesia Simulation Training (VAST) aims to overcome barriers limiting widespread application of simulation-based education (SBE) in resource-limited environments, providing immersive, low-cost, multidisciplinary SBE and simulation facilitator training. Read More

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Case studies for implementing MCDA for tender and purchasing decisions in hospitals in Indonesia and Thailand.

J Pharm Policy Pract 2021 Jun 14;14(1):52. Epub 2021 Jun 14.

Abbott Laboratories ltd, No.1, Q House Lumpini, 30th & 33rd Floor, South Sathorn Road, Thungmahamek, Sathorn, Bangkok, 10120, Thailand.

Background: A multi-criteria decision analysis (MCDA) approach has been suggested for helping purchasers in low- and middle-income countries in an evidence-based assessment of multi-source pharmaceuticals to mitigate potential adverse consequences of price-based decisions on patient access to effective medicines. Six workshops for developing MCDA-instruments for purchasing were conducted in Indonesia, Kazakhstan, Thailand, and Kuwait in 2017-2020. In Indonesia and Thailand, two pilot-initiatives aimed to implement the instruments for hospital drug purchasing decisions. Read More

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Strategies for enhancing terpenoids accumulation in microalgae.

Appl Microbiol Biotechnol 2021 Jun 14. Epub 2021 Jun 14.

School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2 Xuelin Road, Qixia District, Nanjing, People's Republic of China.

Terpenoids represent one of the largest class of chemicals in nature, which play important roles in food and pharmaceutical fields due to diverse biological and pharmacological activities. Microorganisms are recognized as a promising source of terpenoids due to its short growth cycle and sustainability. Importantly, microalgae can fix inorganic carbon through photosynthesis for the growth of themselves and the biosynthesis of various terpenoids. Read More

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Evaluation of the Effectiveness of Soil Streptomyces Isolates for Induction of Plant Resistance Against Tomato mosaic virus (ToMV).

Curr Microbiol 2021 Jun 14. Epub 2021 Jun 14.

Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt.

The ability of various Streptomyces isolates obtained from soil to induce systemic resistance in tomato (Solanum lycopersicum cv. Supra) plant against Tomato mosaic virus (ToMV) was characterized in current study. Importantly, of nine Streptomyces isolates tested herein, the culture filtrate (CF) of one isolate, designated as Streptomyces ovatisporus LC597360, was the most effective. Read More

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Assessing Swarming of Aerobic Bacteria from Human Fecal Matter.

Bio Protoc 2021 May 5;11(9):e4008. Epub 2021 May 5.

Department of Medicine, Genetics, and Molecular Pharmacology, Albert Einstein College of Medicine Bronx, NY, USA.

Swarming - swift movement across a surface via flagella propulsion - is a unique property of many bacteria. The role of swarming, particularly among bacterial populations of the human gut microbiome, is not yet fully understood; although, it is becoming an area of increased scientific and clinical inquiry. To further characterize bacterial swarming in human health, an effective assay for swarming that utilizes complex material, such as fecal matter, is necessary. Read More

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Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension.

Bio Protoc 2021 Apr 20;11(8):e3998. Epub 2021 Apr 20.

The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, 27100 Pavia, Italy.

Recombinant proteins are an essential milestone for a plethora of different applications ranging from pharmaceutical to clinical, and mammalian cell lines are among the currently preferred systems to obtain large amounts of proteins of interest due to their high level of post-translational modification and manageable large-scale production. In this regard, human embryonic kidney 293 (HEK293) cells constitute one of the main standard lab-scale mammalian hosts for recombinant protein production since these cells are relatively easy to handle, scale-up, and transfect. Here, we present a detailed protocol for the cost-effective, reproducible, and scalable implementation of HEK293 cell cultures in suspension (suitable for commercially available HEK293 cells, HEK293-F) for high-quantity recombinant production of secreted soluble multi-domain proteins. Read More

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Metformin Reverses Hashimoto's Thyroiditis by Regulating Key Immune Events.

Front Cell Dev Biol 2021 28;9:685522. Epub 2021 May 28.

Department of Endocrinology, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Background: Hashimoto's thyroiditis (HT) is a common autoimmune disease characterized by high levels of thyroid peroxidase antibody (TPOAb) and thyroid globulin antibody (TgAb) as well as infiltration of lymphocytes in thyroid. In recent years, metformin has been proven to be effective in a variety of autoimmune diseases, such as systemic lupus erythematosus, rheumatoid arthritis and multiple sclerosis.

Methods: This study systematically explored the therapeutic effect of metformin on HT and its underlying mechanism by comprehensively utilizing methods including animal model, cell culture and differentiation, mRNA sequencing and 16S rRNA sequencing. Read More

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Cardiac Mesenchymal Cells Cultured at Physiologic Oxygen Tension Have Superior Therapeutic Efficacy in Heart Failure Caused by Myocardial Infarction.

Front Cell Dev Biol 2021 26;9:662415. Epub 2021 May 26.

Department of Medicine, Institute of Molecular Cardiology, University of Louisville, Louisville, KY, United States.

Stem/progenitor cells are usually cultured at atmospheric O tension (21%); however, since physiologic O tension in the heart is ∼5%, using 21% O may cause oxidative stress and toxicity. Cardiac mesenchymal cells (CMCs), a newly discovered and promising type of progenitor cells, are effective in improving left ventricle (LV) function after myocardial infarction (MI). We have previously shown that, compared with 21% O, culture at 5% O increases CMC proliferation, telomerase activity, telomere length, and resistance to severe hypoxia . Read More

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Prevention and Control of Streptococcosis in Tilapia Culture: A Systematic Review.

J Aquat Anim Health 2021 Jun 14. Epub 2021 Jun 14.

Section of Aquatic Medicine and Nutrition, Faculty of Veterinary Medicine and Biosciences, Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences, Adamstuen Campus, Post Office Box 369, Oslo, 0102, Norway.

Tilapia culture is a very promising industry within the aquaculture sector. However, disease outbreaks have continued to threaten the industry, causing serious economic losses among the producers. Streptococcosis has become the major bacterial disease affecting tilapia production in most regions of the world. Read More

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Outcomes for Patients with Urinary Tract Infection After an Initial Intravenous Antibiotics Dose Before Emergency Department Discharge.

Infect Dis Ther 2021 Jun 14. Epub 2021 Jun 14.

Department of Emergency Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, 833, Taiwan.

Introduction: To investigate the effect of single-dose intravenous antibiotics before emergency department (ED) discharge on the outcomes of patients with urinary tract infections (UTIs).

Methods: This is a retrospective study conducted at the EDs of three medical centers. Patients aged over 18 years who presented to the ED with UTI and were discharged without admission between January 1, 2016 and December 31, 2017 were evaluated. Read More

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Bone Cement Internal Auditory Canal Reconstruction to Reduce CSF Leak After Vestibular Schwannoma Retrosigmoid Approach.

Otol Neurotol 2021 Jun 11. Epub 2021 Jun 11.

Johns Hopkins University, School of Medicine Johns Hopkins School of Medicine, Department of Otolaryngology-Head and Neck Surgery Johns Hopkins School of Medicine, Department of Neurosurgery, Baltimore, MD.

Objective: To describe rates of cerebrospinal fluid (CSF) rhinorrhea after reconstruction of the IAC with calcium phosphate bone cement during retrosigmoid resections of vestibular schwannomas.

Methods: A retrospective chart review of 177 patients who underwent retrosigmoid craniotomy and opening of the internal auditory canal for resection of a vestibular schwannoma between January 2016 and September 2019 at a tertiary referral center. Patients with other cerebellopontine angle tumor histology, neurofibromatosis type II, or those undergoing revision surgeries were excluded. Read More

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The effectiveness of two levels of active office interventions to reduce sedentary behavior in office workers: a mixed-method approach.

Arch Environ Occup Health 2021 Jun 14:1-10. Epub 2021 Jun 14.

Faculty of Sports Science, Chulalongkorn University, Bangkok, Thailand.

Sedentary behavior (SB) rates are rising globally, especially during working hours. This research focused on the effectiveness of two levels of active office interventions to reduce SB in office workers. Participants were 78 nonacademic university employees divided into a control (CON) group and an intervention (INT) group. Read More

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Simulation With Standardized Patients Designed as Interventions to Develop Nursing Students' Cultural Competence: A Systematic Review.

J Transcult Nurs 2021 Jun 12:10436596211023968. Epub 2021 Jun 12.

Burapha University, Chon Buri, Thailand.

Introduction: Nursing education is challenged to improve students' cultural competence. Clinical simulation using standardized patients (SPs) may be an effective learning intervention. This systematic review was to identify current evidence on the use of simulations with SPs as learning interventions that have been developed to improve nursing students' cultural competence and on the effectiveness of those interventions. Read More

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The picture of smoking in my mind: a need for effective anti-smoking public service announcements based on the self-construals of smokers.

Glob Health Promot 2021 Jun 12:17579759211015178. Epub 2021 Jun 12.

Department of Business Administration, Middle East Technical University, Turkey.

This qualitative study sought to understand the behaviours of smokers with independent and interdependent self-construals to design effective anti-smoking public service announcements (PSAs). Findings from the participants' narratives and drawings suggest that individualistic message content (focused on the individual smoker) can be effective for smokers with independent self-construal, while collectivistic message content (focused on a smoker group) can be effective for smokers with interdependent self-construal to increase their quitting intentions. This study also revealed characteristics of the smoker groups in terms of routines, meanings and symbolic values of smoking, which can be used to enrich the contents for anti-smoking PSAs. Read More

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Fit mothers for a healthy future: Breaking the intergenerational cycle of non-alcoholic fatty liver disease with maternal exercise.

Eur J Clin Invest 2021 Jun 13:e13596. Epub 2021 Jun 13.

Laboratory of Metabolism and Exercise (LaMetEx), Research Centre in Physical Activity, Health and Leisure (CIAFEL), Laboratory for Integrative and Translational Research in Population Health (ITR), Faculty of Sport, University of Porto, Porto, Portugal.

SPECIAL ISSUE: 'FOIEGRAS-Bioenergetic Remodelling in the Pathophysiology and Treatment of Non-Alcoholic Fatty Liver Disease'.

Background: Non-alcoholic fatty liver disease (NAFLD) emerges as significant health burden worldwide. Lifestyle changes, unhealthy dietary habits and physical inactivity, can trigger NAFLD development. Read More

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N-acetylcysteine, xCT and suppression of Maxi-chloride channel activity in human placenta.

Placenta 2021 Jun 5;110:46-55. Epub 2021 Jun 5.

Faculty of Medicine, University of Southampton, UK; Institute for Life Sciences, University of Southampton, UK.

Introduction: Placental oxidative stress features in pregnancy pathologies but in clinical trials antioxidant supplementation has not improved outcomes. N-acetylcysteine (NAC) stimulates glutathione production and is proposed as a therapeutic agent in pregnancy. However, key elements of N-acetylcysteine biology, including its cellular uptake mechanism, remains unclear. Read More

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Lactic fermentation of cooked, comminuted mussel, Perna canaliculus.

Food Microbiol 2021 Oct 14;99:103829. Epub 2021 May 14.

School of Science, AUT University, Auckland, New Zealand.

The endogenous microflora of mussels, filter feeders, can include pathogens with resulting food safety concerns. The aim was to develop a cook-then-ferment technology to extend shelf life and safety of a ready-to-eat mussels. Only after cooking to destroy the mussel's endogenous microflora could an edible product be made as determined by pH decline after fermentation and the fate of common pathogens. Read More

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October 2021

How organizational culture influences holistic review: a qualitative multiple case study.

Adv Health Sci Educ Theory Pract 2021 Jun 12. Epub 2021 Jun 12.

Edson College of Nursing and Health Innovation, Arizona State University, Downtown Phoenix Campus, Phoenix, AZ, USA.

Studies primarily involving single health professions programs suggest that holistic review in admissions can increase underrepresented minority (URM) representation among admitted students. However, data showing little improvement in the overall proportion of URMs in many health professions, despite widespread use of holistic review, suggest that relatively few programs using holistic review admit substantial proportions of underrepresented minorities. Therefore, more research is needed to understand factors that facilitate holistic review practices that successfully promote diverse student enrollment. Read More

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Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue.

Sci Rep 2021 Jun 11;11(1):12409. Epub 2021 Jun 11.

Veterinary Pharmacology and Stem Cell Research Laboratory, Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

The trend of regenerative therapy for diabetes in human and veterinary practices has conceptually been proven according to the Edmonton protocol and animal models. Establishing an alternative insulin-producing cell (IPC) resource for further clinical application is a challenging task. This study investigated IPC generation from two practical canine mesenchymal stem cells (cMSCs), canine bone marrow-derived MSCs (cBM-MSCs) and canine adipose-derived MSCs (cAD-MSCs). Read More

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In-vitro and in-vivo study of the interference between Rift Valley fever virus (clone 13) and Sheeppox/Limpy Skin disease viruses.

Sci Rep 2021 Jun 11;11(1):12395. Epub 2021 Jun 11.

R&D Virology, MCI Santé Animale, Lot. 157, Z I, Sud-Ouest (ERAC), B.P. 278, 28810, Mohammedia, Morocco.

Viral interference is a common occurrence that has been reported in cell culture in many cases. In the present study, viral interference between two capripox viruses (sheeppox SPPV and lumpy skin disease virus LSDV in cattle) with Rift Valley fever virus (RVFV) was investigated in vitro and in their natural hosts, sheep and cattle. A combination of SPPV/RVFV and LSDV/RVFV was used to co-infect susceptible cells and animals to detect potential competition. Read More

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Decisional tool for cost of goods analysis of bioartificial liver devices for routine clinical use.

Cytotherapy 2021 Jun 8. Epub 2021 Jun 8.

Department of Biochemical Engineering, Advanced Centre for Biochemical Engineering, University College London, London, UK. Electronic address:

Background Aims: Bioartificial liver devices (BALs) are categorized as advanced therapy medicinal products (ATMPs) with the potential to provide temporary liver support for liver failure patients. However, to meet commercial demands, next-generation BAL manufacturing processes need to be designed that are scalable and financially feasible. The authors describe the development and application of a process economics decisional tool to determine the cost of goods (COG) of alternative BAL process flowsheets across a range of industrial scales. Read More

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Ultrasonic Instrument Effects on Different Implant Surfaces: Profilometry, Energy-Dispersive X-ray Spectroscopy, and Microbiology In Vitro Study.

Int J Oral Maxillofac Implants 2021 May-Jun;36(3):520-528

Purpose: To assess the effects of grade IV titanium ultrasonic tip instrumentation on different grade IV titanium implant surfaces and compare the decontamination of different implant surfaces using chlorhexidine, blue laser, or ozone.

Materials And Methods: Profilometry and energy-dispersive x-ray spectroscopy (EDS) analyses were performed on smooth, laser-micropatterned, and sandblasted grade IV titanium sample disks before (t) and after (t) ultrasonic instrumentation with an ultrasonic grade IV titanium tip. Samples were also incubated with a Streptococcus sanguinis culture. Read More

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A chondrosphere-based scaffold free approach to manufacture an in-vitro articular cartilage model.

Tissue Eng Part A 2021 Jun 11. Epub 2021 Jun 11.

Newcastle University, 5994, School of Engineering, Claremont Road, Newcastle upon Tyne, Tyne and Wear, United Kingdom of Great Britain and Northern Ireland, NE1 7RU;

In-vitro engineering of human articular cartilage (AC) is a regenerative medicine challenge. The main objective of this study was the development of a repeatable scaffold-free in-vitro model of chondrocyte spheroid-based treatments of cartilage defects, to allow for systematic study and further optimisation of this type of treatment. Human articular chondrocytes (HC) and immortalised mesenchymal cells differentiated in chondrocytes (Y201-Cs) were cultured in round-bottom 96-well plates to produce multicellular spheroids and their growth kinetics and viability was evaluated over 7 days of culture. Read More

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miR-498 inhibits autophagy and M2-like polarization of tumor-associated macrophages in esophageal cancer via MDM2/ATF3.

Epigenomics 2021 Jun 11. Epub 2021 Jun 11.

Department of Anatomy, School of Basic Medicine, North Sichuan Medical College, Nanchong, 637000, Sichuan, PR China.

Structured abstract To elucidate the effect of miRNA (miR)-498 on autophagy and M2-like macrophage polarization in esophageal cancer. Autophagy was evaluated in esophageal cancer. Macrophage markers specific for M1- or M2-like phenotype were determined. Read More

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Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment.

Clin Oral Investig 2021 Jun 11. Epub 2021 Jun 11.

Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School - State University of Campinas, UNICAMP, Av Limeira 901, Piracicaba, São Paulo, Brazil.

Objectives: This study aimed to detect the presence of specific bacteria and to evaluate the levels of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in symptomatic necrotic root canals associated with acute apical abscess (symptomatic group - GI). It also aimed to compare the findings with those presented by asymptomatic necrotic root canals (asymptomatic group - GII) in the different stages of the endodontic treatment.

Materials And Methods: Microbiological samples were collected from 20 root canals, including purulent collection from acute apical abscesses, before and after chemo-mechanical preparation (CMP) preparation (CMP) with chlorhexidine gel 2% and after 30 days of intracanal medication (ICM) with (Ca[OH]) + chlorhexidine. Read More

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Improving Patient Safety and Patient-Provider Communication.

Perspect ASHA Spec Interest Groups 2019 Oct 31;4(5):1017-1027. Epub 2019 Oct 31.

Voxello, Coralville, IA.

Purpose: Many hospitalized patients experience barriers to effective patient-provider communication that can negatively impact their care. These barriers include difficulty physically accessing the nurse call system, communicating about pain and other needs, or both. For many patients, these barriers are a result of their admitting condition and not of an underlying chronic disability. Read More

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October 2019