Biomater Sci 2021 Apr 16. Epub 2021 Apr 16.
Texas Heart Institute, 6770 Bertner Avenue, MC 1-135, Houston, TX 77030, USA. and RegenMedix Consulting LLC, Houston, TX 77030, USA.
New robust and reproducible differentiation approaches are needed to generate induced pluripotent stem cell (iPSC)-derived cardiomyocytes of specific subtypes in predictable quantities for tissue-specific disease modeling, tissue engineering, and eventual clinical translation. Here, we assessed whether powdered decellularized extracellular matrix (dECM) particles contained chamber-specific cues that could direct the cardiac differentiation of human iPSCs toward an atrial phenotype. Human hearts were dissected and the left ventricle (LV) and left atria (LA) were isolated, minced, and decellularized using an adapted submersion decellularization technique to generate chamber-specific powdered dECM. Read More