315 results match your criteria cryopreservation rapid-freezing


Post-rewarming Developmental Competence of in vitro Produced Buffalo (Bubalis Bubalis) Embryos Vitrified Using the Solid Surface Technique.

Cryo Letters 2021 Jan-Feb;42(1):13-18

Animal Biotechnology Centre, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.

Background: Vitrification is an ultra-rapid freezing technique for germplasm preservation under high salt concentration with very short exposure time.

Objective: To assess the post-thawed developmental potential of in vitro-produced buffalo embryos vitrified by solid surface technique using different concentrations of cryoprotectants.

Materials And Methods: The slaughterhouse derived oocytes were in vitro matured and fertilized with epididymal sperm. Read More

View Article and Full-Text PDF

Synergistic Ice Inhibition Effect Enhances Rapid Freezing Cryopreservation with Low Concentration of Cryoprotectants.

Adv Sci (Weinh) 2021 Mar 29;8(6):2003387. Epub 2021 Jan 29.

Department of Electronic Science and Technology University of Science and Technology of China No. 96 Road Jinzhai Hefei Anhui 230027 China.

Despite recent advances in controlling ice formation and growth, it remains a challenge to design anti-icing materials in various fields from atmospheric to biological cryopreservation. Herein, tungsten diselenide (WSe)-polyvinyl pyrrolidone (PVP) nanoparticles (NPs) are synthesized through one-step solvothermal route. The WSe-PVP NPs show synergetic ice regulation ability both in the freezing and thawing processes. Read More

View Article and Full-Text PDF

Influence of circulating testosterone concentration on sperm cryoresistance: The ibex as an experimental model.

Andrology 2021 Mar 9. Epub 2021 Mar 9.

Department of Animal Reproduction, INIA, Madrid, Spain.

Background: Recent studies have noted that the circulating testosterone concentration may affect the ability of spermatozoa to survive cryopreservation. However, few attempts to confirm such a relationship have been made. Wild ruminant species have very marked seasonal changes in their reproductive function and strong annual changes in their plasma testosterone concentration. Read More

View Article and Full-Text PDF

Whole genome integrity and enhanced developmental potential in ram freeze-dried spermatozoa at mild sub-zero temperature.

Sci Rep 2020 11 2;10(1):18873. Epub 2020 Nov 2.

Faculty of Veterinary Medicine, University of Teramo, Street R. Balzarini 1, Campus Coste Sant'Agostino, 64100, Teramo, Italy.

Freeze-dried spermatozoa typically shows a reduction in fertility primarily due to the DNA damage resulting from the sublimation process. In order to minimize the physical/mechanical damage resulting from lyophilization, here we focused on the freezing phase, comparing two cooling protocols: (i) rapid-freezing, where ram sperm sample is directly plunged into liquid nitrogen (LN-group), as currently done; (ii) slow-freezing, where the sample is progressively cooled to - 50 °C (SF-group). The spermatozoa dried in both conditions were analysed to assess residual water content by Thermal Gravimetric Analysis (TGA) and DNA integrity using Sperm Chromatin Structure Assay (SCSA). Read More

View Article and Full-Text PDF
November 2020

Comparison of rapid freezing versus vitrification for human sperm cryopreservation using sucrose in closed straw systems.

Cell Tissue Bank 2020 Dec 1;21(4):667-673. Epub 2020 Sep 1.

Key Laboratory of Fertility Preservation, School of Life Sciences and Technologies, Sanquan College of Xinxiang Medical University, Xinxiang, 453003, China.

Rapid freezing and vitrification using sucrose are two simple and cost-effective sperm cryopreservation methods. However, it is still unclear which method is better and what the optimal concentration of sucrose is. This study aimed to determine the optimal sucrose concentration for human sperm cryopreservation and compare the cryoprotective effects of rapid freezing versus vitrification using different closed straw systems in terms of sperm motility and DNA integrity. Read More

View Article and Full-Text PDF
December 2020

Comparison of the effects of three cryoprotectants on the cryopreservation of mouse subcutaneous tissue under different conditions.

Exp Ther Med 2020 Oct 29;20(4):3285-3289. Epub 2020 Jul 29.

College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.

The subcutaneous tissue of animals contains different cell types, and different cells have different requirements for cryopreservation. This establishes obstacles that need to be overcome in the clinical application of tissue preservation. In the present study, the effects of different freezing rates and various concentrations of cryoprotectants on the cryopreservation of subcutaneous tissue of mice were compared, and these results provided basic research data that can be used to explore the optimal cryopreservation method for tissue. Read More

View Article and Full-Text PDF
October 2020

Studies on the basic issues relevant to sperm cryopreservation in humans.

Ther Adv Reprod Health 2020 Jan-Dec;14:2633494120909375. Epub 2020 May 22.

Key Laboratory of Fertility Preservation, School of Life Sciences and Technologies, Sanquan College of Xinxiang Medical University, Xinxiang 453003, China.

Rapid freezing and vitrification are becoming popular for sperm freezing in humans; however, basic and critical issues relevant to sperm cryopreservation remain to be resolved. The aims of the present study were to study the effects of osmolality of freezing medium, sperm concentrations, thawing methods, and sugars (sucrose and trehalose) on sperm motility and DNA integrity by rapid freezing using 0.5 ml standard straws loaded with 100 µl sperm each. Read More

View Article and Full-Text PDF

[Optimization of cryopreservative method for human fetal brain neural stem cells-derived oligodendrocyte precursor cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2020 Mar;36(3):248-254

Laboratory of Pediatrics, Sixth Medical Center, PLA General Hospital, Beijing 100048, China. *Corresponding author, E-mail:

Objective To explore the impact of various conditions during cryopreservation on the survival rate of oligodendrocyte precursor cells (OPCs) derived from human fetal neural stem cells. Methods We compared the cell viability of oligodendrocyte precursors harvested with or without digestion. Then we tested the impact of 3 factors during cryopreservation, freezing solutions (solution with 70 mL/L DMSO and 930 mL/L FBS; solution with 70 mL/L DMSO, 300 mL/L FBS and OPC culture medium; solution with 70 mL/L DMSO, 300 mL/L FBS, 0. Read More

View Article and Full-Text PDF

Hydrogel Microfiber Encapsulation Enhances Cryopreservation of Human Red Blood Cells with Low Concentrations of Glycerol.

Biopreserv Biobank 2020 Jun 21;18(3):228-234. Epub 2020 Apr 21.

Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, China.

Cryopreservation of red blood cells (RBCs) has been studied as a typical example of cryobiology methodology. To date, a mature and long-term cryopreservation process for RBCs has been developed, which has the weakness of complicated procedures due to high concentrations of glycerol (Gly). Therefore, it is still a research focus to find a new method for cryopreservation of RBCs to reduce the concentrations of cryoprotectants (CPAs). Read More

View Article and Full-Text PDF

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation.

Biopreserv Biobank 2020 Jun 13;18(3):235-243. Epub 2020 Apr 13.

Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid, Mossoró, Brazil.

Systematic cryo-banking of reproductive tissues could enhance reproductive management and ensure sustainability of rare mammalian genotypes. Testicular tissues contain a vast number of germ cells, including at early stages (spermatogonia and spermatocytes), that can potentially develop into viable spermatozoa after grafting or culture , and the resulting sperm cells then can be used for assisted reproductive techniques. The objective of this review was to describe current advances, limitations, and perspectives related to the use of testicular tissue preservation as a strategy for the conservation of male fertility. Read More

View Article and Full-Text PDF

Introduction: Frozen 2: an update on cryopreserved embryo transfer in the era of vitrification.

Fertil Steril 2020 02;113(2):239-240

Division of Reproductive Endocrinology & Infertility, Department of Obstetrics & Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, California. Electronic address:

Cryopreservation of reproductive tissues has recently reached an unprecedented level of efficiency. Whereas sperm cryopreservation had been successfully practiced for many years, until relatively recently, cryopreservation of embryos resulted in the loss of many potential implantations, when compared to fresh transfer. Embryo survival rates of approximately 80% with slow freezing methods were common, and the subsequent transfer of frozen-thawed embryos was associated with decreased implantation rates, despite good endometrial preparation techniques. Read More

View Article and Full-Text PDF
February 2020

Elective Egg Freezing for Non-Medical Reasons: Scientific Impact Paper No. 63.

BJOG 2020 08 26;127(9):e113-e121. Epub 2020 Feb 26.

Although a woman's fertility declines markedly in her late-30s and early-40s, gradually more and more women start a family at this stage of their lives, with the average age of childbirth progressively increasing. More women are storing their eggs (oocytes) to give them the potential opportunity to have a baby in the future. Nonetheless, the number of egg freezing cycles accounts for less than 2% of IVF cycles, and the number of cycles using stored eggs is even lower. Read More

View Article and Full-Text PDF

Sequential interval micro-droplet loading in closed hemi-straw carrier system: A convenient and efficient method for ultra-rapid cryopreservation in extreme oligozoospermia.

Cryobiology 2020 04 11;93:75-83. Epub 2020 Feb 11.

Jinling Hospital Department Reproductive Medical Center, Southern Medical University, Nanjing, 210002, China. Electronic address:

Cryopreservation of human spermatozoa with low concentration while maintaining adequate post-thawing motility remains a major challenge for male fertility preservation. A convenient and efficient ultra-rapid freezing method for small amounts of human spermatozoa in a closed Hemi-Straw carrier system (CHS) was developed. Spermatozoa from 60 healthy men were involved in a parameter refining test and another 15 extreme oligozoospermic specimens were assigned to a verification test. Read More

View Article and Full-Text PDF

Canthaxanthin protects human sperm parameters during cryopreservation.

Andrologia 2019 Nov 11;51(10):e13389. Epub 2019 Aug 11.

Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Different antioxidants have been introduced to reduce oxidative stress during the cryopreservation. The main goal of this study was to evaluate the effects of canthaxanthin on human sperm parameters during the freeze-thaw process. This study was performed on 25 normozoospermic semen samples dividing into five groups including 0, 0. Read More

View Article and Full-Text PDF
November 2019

Current perceptions and decisions of patients about cryopreserved embryos in Argentina.

Rev Fac Cien Med Univ Nac Cordoba 2019 06 19;76(2):124-130. Epub 2019 Jun 19.

CONICET. Universidad de Buenos Aires. Facultad de Psicología.

Introduction: Worldwide there is an increase in the number of assisted reproduction treatments. The developments in culture media and drugs, associated with the occurrence of rapid freezing or vitrification techniques has improved the procedures encouraging transfers of a single embryo per cycle. This generates a greater number of viable surplus embryos. Read More

View Article and Full-Text PDF

Vitrification of stallion sperm using 0.25 ml straws: Effect of volume, concentration and carbohydrates (sucrose/trehalose/raffinose).

Anim Reprod Sci 2019 Jul 21;206:69-77. Epub 2019 May 21.

Veterinary Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, Spain. Electronic address:

Sperm vitrification is a rapid freezing method in which carbohydrates are used as cryoprotectants. The aim of this study was to determine the optimal volume, concentration and type of carbohydrates for stallion sperm vitrification using 0.25 ml straws in comparison to conventional freezing. Read More

View Article and Full-Text PDF

Can spray freeze-drying improve the re-dispersion of crystalline nanoparticles of pure naproxen?

Int J Pharm 2019 Jun 24;564:293-298. Epub 2019 Apr 24.

Division of Pharmaceutics, Friedrich-Alexander-University, Erlangen, Germany. Electronic address:

Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose. The particle size of the rehydrated powders was determined using static light scattering/Mie analysis. The nanoparticles present in the SFD powders were aggregated but could be dispersed on re-dispersion with water and stirring either with or without additional ultrasonic treatment. Read More

View Article and Full-Text PDF

Effectiveness of ultra-rapid cryopreservation of sperm from endangered species, examined by morphometric means.

Theriogenology 2019 Apr 1;129:160-167. Epub 2019 Mar 1.

Departamento de Reproducción Animal, SGIT-INIA, Avda. Puerta de Hierro km 5.9, 28040, Madrid, Spain. Electronic address:

This study compares the effectiveness of the ultra-rapid and conventional freezing of sperm from captive bovids, giraffids, cervids, ursids, a cercopithecid, a delphinid and a phascolarctid. The relationship between sperm head dimensions and cryosurvival was also examined. Compared to conventional freezing, the ultra-rapid freezing of epididymal sperm from the dama gazelle, giraffe and brown bear returned higher cryoresistance ratios (CR, the ratio, in percentage, between the value of the variable after thawing/value before thawing) for sperm viability and motility. Read More

View Article and Full-Text PDF

Effect of Different Extenders and Dilution Ratios on the Spermatological and Fertilization Parameters of Cyprinus carpio During Cryopreservation.

Biopreserv Biobank 2019 Apr 11;17(2):157-162. Epub 2019 Feb 11.

3 Department of Animal Genetics and Breeding, Veterinary College & Research Institute, Tamil Nadu Animal Sciences and Veterinary University, Chennai, India.

Production of quality seeds is a critical need, and cryopreservation serves as an important biotechnological tool in achieving that goal. In the present study, the effects of different extenders and dilution ratios on the spermatological and fertilization parameters of cryopreserved milt were evaluated in common carp brooders. Milt was collected from Cyprinus carpio brooders and diluted and cryopreserved at three dilution ratios such as 1:40, 1:80, and 1:120, using Freshwater Fish Saline (FWFS) and Modified Fish Ringer as extenders and dimethyl sulfoxide as cryoprotectant (90:10). Read More

View Article and Full-Text PDF

Cryopreservation of human spermatozoa by vitrification versus conventional rapid freezing: Effects on motility, viability, morphology and cellular defects.

Eur J Obstet Gynecol Reprod Biol 2019 Mar 5;234:14-20. Epub 2019 Jan 5.

Department of OBGYN, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen Street, Hue, Viet Nam; Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, 06 Ngo Quyen Street, Hue, Viet Nam.

Objectives: Sperm cryopreservation has great potential for male infertility treatment as used in assisted reproduction technology (ART). There are a variety of cryopreservation methods in order to preserve sperm in a long term. Although conventional freezing and vitrification now are used widely, they have damage on sperm parameters as well as sperm DNA integrity. Read More

View Article and Full-Text PDF

Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender.

Reprod Domest Anim 2019 Mar 8;54(3):551-559. Epub 2019 Jan 8.

Department of Animal Physiology, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria.

Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk (TNM) extender was examined in this study. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris-based extenders containing different levels of TNM (0, 5, 10, 15 and 20 ml/100 ml extender). The diluted semen samples were subjected to slow and rapid freezing for a period of 7 days and thereafter evaluated for sperm functional attributes (percentage motility, acrosome integrity, membrane integrity, abnormality and livability) and oxidative stress (malondialdehyde [MDA] concentration and acrosin activity) parameters. Read More

View Article and Full-Text PDF

The Effect of Caffeic Acid on Spermatogonial Stem Cell-type A Cryopreservation.

Rep Biochem Mol Biol 2018 Oct;7(1):85-93

Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: Cancer treatment methods can lead to male infertility .in this regard, cryopreservation of spermatogonial stem cells (SSC) and cell-to-person transplantation after the course of treatment to resolve the problem of infertility is a good one. The cryopreservation of SSC is an important process as it can help on the return of spermatogenesis. Read More

View Article and Full-Text PDF
October 2018

Modification of cellular membranes conveys cryoprotection to cells during rapid, non-equilibrium cryopreservation.

Authors:
Jan Huebinger

PLoS One 2018 10;13(10):e0205520. Epub 2018 Oct 10.

Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.

Rapid cooling and re-warming has been shown promising to cryopreserve living cells, which cannot be preserved by conventional slow freezing methods. However, success is limited by the cytotoxicity of highly concentrated cryoprotective agents. Recent results have shown that cryoprotective agents do not need to suppress intracellular ice crystals completely to allow for survival after cryopreservation. Read More

View Article and Full-Text PDF

Rapid freezing versus Cryotop vitrification of mouse two-cell embryos.

Clin Exp Reprod Med 2018 Sep 3;45(3):110-115. Epub 2018 Sep 3.

Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.

Objective: To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts.

Methods: Two-cell mouse embryos were randomly allocated into three groups: a non-frozen control group (group 1, n=300), a group that underwent Cryotop vitrification (group 2, n=300), and a group that underwent our in-house freezing method (group 3, n=300).

Results: There were no significant differences between groups 2 and 3 in the immediate survival rate (96. Read More

View Article and Full-Text PDF
September 2018

Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation.

JBRA Assist Reprod 2018 Nov 1;22(4):331-337. Epub 2018 Nov 1.

Fertilidad San Isidro, Buenos Aires, Argentina.

Objective: Cryopreservation of human spermatozoa is fundamental in assisted reproductive technology. At present, slow freezing techniques are widely used for sperm cryopreservation. Recently, sperm vitrification has been proposed as an alternative to slow freezing. Read More

View Article and Full-Text PDF
November 2018

Modulating Intracellular Ice Growth with Cell-Permeating Small-Molecule Ice Recrystallization Inhibitors.

Langmuir 2019 06 7;35(23):7452-7458. Epub 2018 Sep 7.

Department of Chemistry and Biomolecular Sciences , University of Ottawa , Ottawa , Ontario K1N 6N5 , Canada.

Ice formation remains central to our understanding of the effects of low temperatures on the biological response of cells and tissues. The formation of ice inside of cells and the net increase in crystal size due to recrystallization during thawing is associated with a loss of cell viability during cryopreservation. Because small-molecule ice recrystallization inhibitors (IRIs) can control the growth of extracellular ice, we sought to investigate the ability of two aryl-glycoside-based IRIs to permeate into cells and control intracellular ice recrystallization. Read More

View Article and Full-Text PDF

Cryopreservation of orchid seeds through rapid and step freezing methods.

F1000Res 2018 20;7:209. Epub 2018 Feb 20.

Carrera de Biotecnología de los Recursos Naturales, Universidad Politécnica Salesiana, Quito, Ecuador.

Ecuador has a great variety of climatic regions that potentiate biodiversity. The family constitutes one of the most important of the country, having identified about 4032 species with a high degree of endemism, therefore the development and research of alternative methods of storage and conservation of species is a strategy of primary interest for researchers and for society in general. In cryopreservation, temperatures reach below -190°C in order to paralyze the chemical reactions and keep the plant material viable for long periods. Read More

View Article and Full-Text PDF
February 2018

Fertility preservation for adolescent and young adult cancer patients in Japan.

Obstet Gynecol Sci 2018 Jul 19;61(4):443-452. Epub 2018 Jun 19.

Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Japan.

Adolescent and young adult (AYA) patients are generally defined as being from 15 to 39 years old. For preservation of fertility in AYA cancer patients, the best-known guideline in this field was released by the American Society of Clinical Oncology (ASCO) in 2006. However, the ASCO guideline is not necessarily applicable to Japanese cancer patients. Read More

View Article and Full-Text PDF

Concentrations of non-permeable cryoprotectants and equilibration temperatures are key factors for stallion sperm vitrification success.

Anim Reprod Sci 2018 Sep 30;196:91-98. Epub 2018 Jun 30.

Department of Reproduction, Centro Militar de Cría Caballar (CCFAS-Ministry of Defense), 05005 Ávila, Spain.

Vitrification is based on rapid freezing by direct exposure of sperm to liquid nitrogen (LN). This study evaluated the effect of non-permeable CPAs and equilibration temperature on stallion sperm quality after vitrification. In Experiment 1, different concentrations of sucrose (20, 50, 100 mM; mmol/L) and bovine serum albumin (BSA 1%, 5%, 10%) were compared including different temperatures for the equilibration (≈22 °C or 5 °C). Read More

View Article and Full-Text PDF
September 2018

Self-Pressurized Rapid Freezing as Cryo-Fixation Method for Electron Microscopy and Cryopreservation of Living Cells.

Curr Protoc Cell Biol 2018 06 11;79(1):e47. Epub 2018 May 11.

Institute for Anatomy and Cell Biology, University of Heidelberg, Heidelberg, Germany.

Reduction or complete prevention of ice crystal formation during freezing of biological specimens is mandatory for two important biological applications: (1) cryopreservation of living cells or tissues for long-term storage, and (2) cryo-fixation for ultrastructural investigations by electron microscopy. Here, a protocol that is fast, easy-to-use, and suitable for both cryo-fixation and cryopreservation is described. Samples are rapidly cooled in tightly sealed metal tubes of high thermal diffusivity and then plunged into a liquid cryogen. Read More

View Article and Full-Text PDF