325,894 results match your criteria crucial

Transient neural network dynamics in cognitive ageing.

Neurobiol Aging 2021 May 14;105:217-228. Epub 2021 May 14.

MRC Cognition & Brain Sciences Unit, University of Cambridge, Cambridge, UK; Department of Psychiatry, University of Cambridge, Cambridge, UK.

It is important to maintain cognitive function in old age, yet the neural substrates that support successful cognitive ageing remain unclear. One factor that might be crucial, but has been overlooked due to limitations of previous data and methods, is the ability of brain networks to flexibly reorganize and coordinate over a millisecond time-scale. Magnetoencephalography (MEG) provides such temporal resolution, and can be combined with Hidden Markov Models (HMMs) to characterise transient neural states. Read More

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The scope of flavin-dependent reactions and processes in the model plant Arabidopsis thaliana.

Phytochemistry 2021 Jun 9;189:112822. Epub 2021 Jun 9.

Institute of Biochemistry, Graz University of Technology, Petersgasse 12/2, 8010, Graz, Austria. Electronic address:

Flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are utilized as coenzymes in many biochemical reduction-oxidation reactions owing to the ability of the tricyclic isoalloxazine ring system to employ the oxidized, radical and reduced state. We have analyzed the genome of Arabidopsis thaliana to establish an inventory of genes encoding flavin-dependent enzymes (flavoenzymes) as a basis to explore the range of flavin-dependent biochemical reactions that occur in this model plant. Expectedly, flavoenzymes catalyze many pivotal reactions in primary catabolism, which are connected to the degradation of basic metabolites, such as fatty and amino acids as well as carbohydrates and purines. Read More

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MUC1 regulates AKT signaling pathway by upregulating EGFR expression in ovarian cancer cells.

Pathol Res Pract 2021 Jun 4;224:153509. Epub 2021 Jun 4.

School of Basic Medical Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China. Electronic address:

MUC1, a type I transmembrane glycoprotein, mediates tumor growth and cellular differentiation in various types of cancers. However, the mechanism of MUCI in ovarian cancer has not been fully clarified. In our study, we have observed that MUC1 can play a crucial role in the development and progression of ovarian cancer and act as a predictive marker. Read More

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Multi-product strategy to enhance the environmental profile of the canning industry towards circular economy.

Sci Total Environ 2021 Jun 5;791:148249. Epub 2021 Jun 5.

CRETUS, Department of Chemical Engineering, Universidade de Santiago de Compostela, 15705 Santiago de Compostela, Spain.

The sustainable and continued production of enough food to feed the entire world's population is one of the main concerns in the food industry. Spain, and in particular Galicia, which is an eminently fishing region characterised by the consumption of large quantities of fish, both fresh and processed, must face the challenge of shifting its seafood productive fabric towards a circular economy. To achieve this objective, the first task is to demonstrate that circular economy principles allow to reduce the environmental impacts associated with seafood production. Read More

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Large-scale evaluation of the Positive and Negative Syndrome Scale (PANSS) symptom architecture in schizophrenia.

Asian J Psychiatr 2021 Jun 3;62:102732. Epub 2021 Jun 3.

Research Division, Institute of Mental Health, Singapore; Stanley Center for Psychiatric Research, The Broad Institute of MIT and Harvard, Cambridge, MA, United States; Feinstein Institute of Medical Research, The Zucker Hillside Hospital, New York, United States. Electronic address:

Although the Positive and Negative Syndrome Scale (PANSS) is widely utilized in schizophrenia research, variability in specific item loading exist, hindering reproducibility and generalizability of findings across schizophrenia samples. We aim to establish a common PANSS factor structure from a large multi-ethnic sample and validate it against a meta-analysis of existing PANSS models. Schizophrenia participants (N = 3511) included in the current study were part of the Singapore Translational and Clinical Research Program (STCRP) and the Clinical Antipsychotic Trials for Intervention Effectiveness (CATIE). Read More

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Escherichia coli Dcu C-dicarboxylate transporters dependent proton and potassium fluxes and FF-ATPase activity during glucose fermentation at pH 7.5.

Bioelectrochemistry 2021 Jun 4;141:107867. Epub 2021 Jun 4.

Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, 1 A. Manoogian str., 0025 Yerevan, Armenia; Scientific-Research Institute of Biology, Yerevan State University, 1 A. Manoogian str., 0025 Yerevan, Armenia; Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, 1 A. Manoogian str., 0025 Yerevan, Armenia. Electronic address:

During fermentation in Escherichia coli succinate is transported via Dcu transporters, encoded dcuA, dcuB, dcuC and dcuD although the role of DcuD protein has not been elucidated yet. It has been shown contribution of Dcu transporters in the N,N'-dicyclohexylcarbodiimide (DCCD) sensitive proton and potassium transport through the cytoplasmic membrane and membrane-associated ATPase activity. Total H efflux was decreased ~ 40% while K uptake was absent in dcuD mutant. Read More

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Biofortification quality in bananas monitored by energy-dispersive X-ray fluorescence and chemometrics.

Food Chem 2021 Jun 2;362:130172. Epub 2021 Jun 2.

São Paulo State University (UNESP), Institute of Chemistry, Group of Alternative Analytical Approaches (GAAA), Bioenergy Research Institute (IPBEN), Av. Prof. Francisco Degni, 55 - Jardim Quitandinha, Araraquara, São Paulo CEP: 14.800-060, Brazil; National Institute of Alternative Technologies for Detection Toxicological Assessment and Removal of Micropollutants and Radioactive Substances (INCT-DATREM), São Paulo State University (UNESP), Institute of Chemistry, Av. Prof. Francisco Degni, 55 - Jardim Quitandinha, Araraquara, São Paulo, CEP: 14.800-060, Brazil. Electronic address:

Biofortification is a nutritional strategy used to enhance nutrients in a variety of staple foods. As bananas and plantains (Musa spp.) are considered staple food in many developing countries, monitoring zinc (Zn) content in biofortified bananas is crucial to ensure this mineral intake. Read More

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Digital health, big data and smart technologies for the care of patients with systemic autoimmune diseases: Where do we stand?

Autoimmun Rev 2021 Jun 9:102864. Epub 2021 Jun 9.

Department of neuroradiology, A. Rothshield Foundation Hospital, Paris, France. Electronic address:

The past decade has seen tremendous development in digital health, including in innovative new technologies such as Electronic Health Records, telemedicine, virtual visits, wearable technology and sophisticated analytical tools such as artificial intelligence (AI) and machine learning for the deep-integration of big data. In the field of rare connective tissue diseases (rCTDs), these opportunities include increased access to scarce and remote expertise, improved patient monitoring, increased participation and therapeutic adherence, better patient outcomes and patient empowerment. In this review, we discuss opportunities and key-barriers to improve application of digital health technologies in the field of autoimmune diseases. Read More

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A biomimetic triple-layered biocomposite with effective multifunction for dura repair.

Acta Biomater 2021 Jun 9. Epub 2021 Jun 9.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China. Electronic address:

Dura mater defect and subsequent cerebrospinal fluid (CSF) leakage usually appear in trauma or neurosurgical procedures and are followed by a series of serious complications and even death. The use of a qualified dura mater substitute with multifunction of leakage blockade, adhesion prevention, and dura reconstruction is one of the promising treatment methods. However, even though some products have been used in the clinic, none of the substitutes achieved the required multifunction. Read More

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Multiscale modeling of bone tissue Mechanobiology.

Bone 2021 Jun 9:116032. Epub 2021 Jun 9.

Multiscale in Mechanical and Biological Engineering, Instituto de Investigación en Ingeniería de Aragón (I3A), Instituto de Investigación Sanitaria Aragón (IIS Aragón), University of Zaragoza, Zaragoza, Spain.

Mechanical environment has a crucial role in our organism at the different levels, ranging from cells to tissues and our own organs. This regulatory role is especially relevant for bones, given their importance as load-transmitting elements that allow the movement of our body as well as the protection of vital organs from load impacts. Therefore bone, as living tissue, is continuously adapting its properties, shape and repairing itself, being the mechanical loads one of the main regulatory stimuli that modulate this adaptive behavior. Read More

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Biglycan in hepatorenal crosstalk Biglycan: a regulator of hepatorenal inflammation and autophagy.

Matrix Biol 2021 Jun 9. Epub 2021 Jun 9.

Institute of Pharmacology and Toxicology, Goethe University, Frankfurt, Germany. Electronic address:

Soluble biglycan, a small leucine-rich proteoglycan, plays a significant role in several pathologies as it has emerged as an extracellular matrix-derived danger-associated molecular pattern. Biglycan is released from the extracellular matrix in response to tissue injury and, as a canonical danger signal, interacts with innate immune receptor, Toll-like receptors 2 and 4, thereby triggering a sustained inflammatory response. Recent evidence indicates that biglycan acts as a molecular switch between inflammation and autophagy by a specific interaction with the Toll-like co-receptor CD14 and CD44, respectively. Read More

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Pregnancy and Viral infections: Mechanisms of fetal damage, diagnosis and prevention of neonatal adverse outcomes from cytomegalovirus to SARS-CoV-2 and Zika virus.

Biochim Biophys Acta Mol Basis Dis 2021 Jun 9:166198. Epub 2021 Jun 9.

Fetal and Perinatal Medicine and Surgery Unit, Medical and Surgical Department of Fetus, Newborn and Infant - "Bambino Gesù" Children's Hospital IRCCS, Rome, Italy.

Some maternal infections, contracted before or during pregnancy, can be transmitted to the fetus, during gestation (congenital infection), during labor and childbirth (perinatal infection) and through breastfeeding (postnatal infection). The agents responsible for these infections can be viruses, bacteria, protozoa, fungi. Among the viruses most frequently responsible for congenital infections are Cytomegalovirus (CMV), Herpes simplex 1-2, Herpes virus 6, Varicella zoster. Read More

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Selecting Software Pipelines for Change in Flortaucipir SUVR: Balancing Repeatability and Group Separation.

Neuroimage 2021 Jun 9:118259. Epub 2021 Jun 9.

Department of Radiology, Mayo Clinic and Foundation, Rochester, MN, USA.

Since tau PET tracers were introduced, investigators have quantified them using a wide variety of automated methods. As longitudinal cohort studies acquire second and third time points of serial within-person tau PET data, determining the best pipeline to measure change has become crucial. We compared a total of 415 different quantification methods (each a combination of multiple options) according to their effects on a) differences in annual SUVR change between clinical groups, and b) longitudinal measurement repeatability as measured by the error term from a linear mixed-effects model. Read More

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?Mini-review:_Guillain Barrè syndrome and myelitis associated with SARS-CoV-2 infection_?

Neurosci Lett 2021 Jun 9:136040. Epub 2021 Jun 9.

Emergency Neurology Unit, IRCCS C. Mondino Foundation, Pavia, Italy.

Despite a likely underestimation due to the many obstacles of the highly infectious, intensive care setting, increasing clinical reports about COVID-19 patients developing acute paralysis for polyradiculoneuritis or myelitis determine additional impact on the disease course and outcome. Different pathogenic mechanisms have been postulated basing on clinical, laboratory and neuroimaging features, and response to treatments. Here we provide an overview with insights built on the available reports. Read More

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Effects of somatic tissue cryopreservation on puma (Puma concolor Linnaeus, 1771) tissue integrity and cell preservation after in vitro culture.

Cryobiology 2021 Jun 9. Epub 2021 Jun 9.

Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoro, RN, Brazil. Electronic address:

Somatic resource banks play a crucial role in the conservation of genetic diversity, allowing for the preservation of biological samples from different populations. Puma somatic cells can be recovered from these banks and used in assisted techniques toward enhancing their multiplication and conservation. In response to the population reduction of this ecologically importance species, we aimed to evaluate the capacity of cryopreservation of somatic tissues on the maintenance of the integrity and quality of the cells recovered after culture, with the aim of establishing a somatic tissue bank that will allow for the safeguarding of a wide genetic sampling of pumas. Read More

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Upregulation of centromere protein K is crucial for lung adenocarcinoma cell viability and invasion.

Adv Clin Exp Med 2021 Jun 11. Epub 2021 Jun 11.

Medical Laboratory, Second Affiliated Hospital of Mudanjiang Medical University, China.

Background: Identification of functional genes or biomarkers may be helpful for developing new treatment strategies in lung adenocarcinoma (LUAD). The centromere protein K (CENPK) gene has been discovered to be overexpressed and could influence tumor progression in several tumor types. However, its role in LUAD has never been revealed. Read More

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Intrinsically Disordered Osteopontin Fragment Orders During Interfacial Calcium Oxalate Mineralization.

Angew Chem Int Ed Engl 2021 Jun 12. Epub 2021 Jun 12.

Max Planck Institute for Polymer Research: Max-Planck-Institut fur Polymerforschung, molecular spectroscopy, GERMANY.

Calcium oxalate (CaC 2 O 4 ) is the major component of kidney stone, a prevalent chronic human disease worldwide. The acidic osteopontin (OPN) protein in human urine can effectively inhibit the growth of CaC 2 O 4 crystals, thereby acting as a potent stone preventer. Previous studies in bulk solution all attest to the importance of binding and recognition of OPN at the CaC 2 O 4 mineral surface, yet molecular level insights into the active interface during CaC 2 O 4 mineralization are still lacking. Read More

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Development of Mitochondria-Targeted Imaging Nanoplatforms by Incorporation of Fluorescent Carbon Quantum Dots.

Ye Zhang Hong Bi

Methods Mol Biol 2021 ;2275:265-277

School of Chemistry and Chemical Engineering, Anhui University, Hefei, China.

Multifunctional nanoplatforms are promising scaffolds for biomedical applications such as bioimaging, chemical/biological sensors, drug delivery, and cancer diagnosis and/or treatments. Mitochondria play crucial roles in metabolism of eukaryotic cells; therefore, mitochondria-targeting molecule such as triphenylphosphonium (TPP) is attached onto the magnetic mesoporous silica nanoparticle ([email protected]). In order to track the nanoparticles, fluorescent carbon quantum dots (CDs) were conjugated to the [email protected] Read More

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January 2021

Mt-fura-2, a Ratiometric Mitochondria-Targeted Ca Sensor. Determination of Spectroscopic Properties and Ca Imaging Assays.

Methods Mol Biol 2021 ;2275:187-215

Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy.

Ca handling by mitochondria is implicated in energy production, shaping of cytosolic Ca rises, and determination of cell fate. It is therefore of crucial interest for researchers to directly measure mitochondrial Ca concentration [Ca] in living cells. Synthetic fluorescent Ca indicators, providing a straightforward loading technique, represents a tempting strategy. Read More

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January 2021

Cardiooncology-dealing with modern drug treatment, long-term complications, and cancer survivorship.

Clin Exp Metastasis 2021 Jun 12. Epub 2021 Jun 12.

Dept. of Cardiology and Angiology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.

Modern treatment strategies have improved prognosis and survival of patients with malignant diseases. The key components of tumor treatment are conventional chemotherapy, radiotherapy, targeted therapies, and immunotherapy. Cardiovascular side-effects may occur in the early phase of tumor therapy or even decades later. Read More

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Immune age and biological age as determinants of vaccine responsiveness among elderly populations: the Human Immunomics Initiative research program.

Eur J Epidemiol 2021 Jun 12. Epub 2021 Jun 12.

Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

The Human Immunomics Initiative (HII), a joint project between the Harvard T.H. Chan School of Public Health and the Human Vaccines Project (HVP), focuses on studying immunity and the predictability of immuneresponsiveness to vaccines in aging populations. Read More

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Identification and Expression Analysis of miR160 and Their Target Genes in Cucumber.

Biochem Genet 2021 Jun 12. Epub 2021 Jun 12.

School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, 453003, China.

miR160 plays a crucial role in various biological processes by regulating their target gene auxin response factor (ARF) in plants. However, little is known about miR160 and ARF in cucumber fruit expansion. Here, 4 Csa-MIR160 family members and 17 CsARFs were identified through a genome-wide search. Read More

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A review on the computational studies of the reaction mechanisms of CO conversion on pure and bimetals of late 3d metals.

J Mol Model 2021 Jun 12;27(7):200. Epub 2021 Jun 12.

Department of Basic Sciences, University of Health and Allied Sciences, Ho, Ghana.

Despite series of experimental studies that reveal unique activities of late 3d transition metals and their role in microorganisms known for CO conversion, these surfaces are not industrially viable yet. An insight into the elementary steps of surface catalytic processes is crucial for effective surface modification and design. The mechanisms of CO transformation into CO, through the reverse water gas shift and methane reforming, are being studied. Read More

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Good Solid-State Electrolytes Have Low, Glass-Like Thermal Conductivity.

Small 2021 Jun 12:e2101693. Epub 2021 Jun 12.

Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Thermal management in Li-ion batteries is critical for their safety, reliability, and performance. Understanding the thermal conductivity of the battery materials is crucial for controlling the temperature and temperature distribution in batteries. This work provides systemic quantitative measurements of the thermal conductivity of three important classes of solid electrolytes (SEs) over the temperature range 150 < T < 350 K. Read More

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Tetra ostial coronary arterial anatomy-A rare pattern in transposition.

J Card Surg 2021 Jun 12. Epub 2021 Jun 12.

Pediatric Cardiac Surgery, Madras Medical Mission, Chennai, India.

Background: Meticulous transfer of coronary arteries is of crucial importance in transposition and determines the success of the switch procedure. This report describes a coronary arterial anatomy consisting of four separate ostia from the two facing sinuses in a 6-month-old infant presenting with transposition and ventricular septal defect.

Conclusion: Being a rare coronary arterial pattern not described in previous coding systems, the surgeon would do well to be aware of this possibility while performing the switch procedure. Read More

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MiR-133a-3p inhibits the malignant progression of esophageal cancer by targeting CDCA8.

J Biochem 2021 Jun 12. Epub 2021 Jun 12.

Department of Thoracic Surgery, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou City, Fujian Province, 350001, China.

Objective: To explore the interaction between miR-133a-3p and CDCA8 in esophageal cancer (EC) and their effect on malignant behavior of EC cells.

Methods: Differential miRNAs and mRNAs were obtained from The Cancer Genome Atlas (TCGA) database. Quantitative real-time PCR (qRT-PCR) was used to detect the expression levels of miR-133a-3p and CDCA8 mRNA in EC cells. Read More

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Developmental nutrition affects the structural integrity of a sexually selected weapon.

Integr Comp Biol 2021 Jun 12. Epub 2021 Jun 12.

Entomology and Nematology Department, University of Florida, Gainesville, Florida.

Males in many species engage in physical combat over access to mates, and sexual selection has led to the evolution of weapons to enhance contest performance. The size of these often-elaborate structures is known to be exquisitely sensitive to nutrition. However, we know very little about the degree to which nutrition affects other attributes of animal weapons that can be crucial to fighting. Read More

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Toward a Better Regeneration through Implant-Mediated Immunomodulation: Harnessing the Immune Responses.

Adv Sci (Weinh) 2021 Jun 12:e2100446. Epub 2021 Jun 12.

Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, 11794, USA.

Tissue repair/regeneration, after implantation or injury, involves comprehensive physiological processes wherein immune responses play a crucial role to enable tissue restoration, amidst the immune cells early-stage response to tissue damages. These cells break down extracellular matrix, clear debris, and secret cytokines to orchestrate regeneration. However, the immune response can also lead to abnormal tissue healing or scar formation if not well directed. Read More

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Swallowing Exercise During Head and Neck Cancer Treatment: Results of a Randomized Trial.

Dysphagia 2021 Jun 11. Epub 2021 Jun 11.

Cancer Late Effects Research Unit (CASTLE), Department of Oncology, Copenhagen University Hospital Rigshospitalet, Blegdamsvej 9, 2100, Copenhagen, Denmark.

The diagnosis and treatment of head and neck cancer (HNC) can have substantial impact on swallowing function, nutritional balance, physical function and quality of life (QoL). Early initiated swallowing exercises are hypothesized to improve swallowing function in HNC patients. The aim was to investigate the effects of swallowing exercises and progressive resistance training (PRT) during radiotherapy on swallowing function, physical function and QoL in patients with pharynx-, larynx-, oral cavity cancer or unknown primary compared to usual care. Read More

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Environmental dimensions of the protein corona.

Nat Nanotechnol 2021 Jun 11;16(6):617-629. Epub 2021 Jun 11.

School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.

The adsorption of biomolecules to the surface of engineered nanomaterials, known as corona formation, defines their biological identity by altering their surface properties and transforming the physical, chemical and biological characteristics of the particles. In the first decade since the term protein corona was coined, studies have focused primarily on biomedical applications and human toxicity. The relevance of the environmental dimensions of the protein corona is still emerging. Read More

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