1,858 results match your criteria crista

Cadaveric Study of Crista Fenestra: Revisited.

J Int Adv Otol 2021 May;17(3):245-250

Department of Otolaryngology-Head and Neck Surgery Alexandria Medical School, Alexandria, Egypt.

Objectives: Since there is great confusion in the literature about the anatomy and terms of the crista fenestra (CF) and the crista semilunaris, this paper is confined to the anatomy of the inferior margin of the round window (RW).

Methods: This study was carried out on 20 cadaveric temporal bones. We measured the maximal height of the RW and the maximum height of the inferior bony edge of the RW, (termed CF type A), in this study. Read More

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Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging.

Chem Sci 2020 Jul 27;11(32):8506-8516. Epub 2020 Jul 27.

Institute of Molecular Medicine, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Peking University Beijing China

Modern fluorescence-imaging methods promise to unveil organelle dynamics in live cells. Phototoxicity, however, has become a prevailing issue when boosted illumination applies. Mitochondria are representative organelles whose research heavily relies on optical imaging, yet these membranous hubs of bioenergy are exceptionally vulnerable to photodamage. Read More

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Normal breast epithelial MCF-10A cells to evaluate the safety of carbon dots.

RSC Med Chem 2021 Mar 10;12(2):245-253. Epub 2020 Dec 10.

Chemistry Research Unit (CIQUP), Faculty of Sciences of University of Porto (FCUP) Rua do Campo Alegre 687 4169-007 Porto Portugal

The human normal breast cell line MCF-10A is being widely used as a model in toxicity studies due to its structural similarity to the normal human mammary epithelium. Over the years, application of carbon dots (C-dots) in biomedicine has been increasing due to their photoluminescence properties, biocompatibility, biosafety and possible applications in bioimaging and as drug carriers. In this work we prepared three different C-dots from the same set of carbon and nitrogen precursors (citric acid and urea, respectively) three distinct bottom-up synthetic routes and their safety was tested against the normal breast cell line MCF-10A. Read More

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Comparison of clockwise and counterclockwise right atrial flutter using high-resolution mapping and automated velocity measurements.

J Cardiovasc Electrophysiol 2021 May 26. Epub 2021 May 26.

Division of Cardiology, Jean Monnet University, Saint-Etienne, France.

Background: Only few studies have been performed that explore the electrophysiological differences between clockwise (CW) and counterclockwise (CCW) right atrial (RA) cavotricuspid isthmus (CTI)-dependent atrial flutter (AFL) using the high-resolution Rhythmia mapping system.

Objectives: We sought to compare CW and CCW CTI-dependent AFL in pure right AFL patients (pts) using the ultra-high-definition (ultra-HD) Rhythmia mapping system and we mathematically developed a cartography model based on automatic velocity RA measurements to identify electrophysiological AFL specificities.

Methods And Results: Thirty-three pts were recruited. Read More

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Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node.

J Vis Exp 2021 May 7(171). Epub 2021 May 7.

University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU); Walter Brendel Center of Experimental Medicine (WBex), LMU Munich; German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA).

Resident cardiac macrophages have been demonstrated to facilitate the electrical conduction in the heart. The physiologic heart rhythm is initiated by electrical impulses generated in sinoatrial node (SAN) and then conducted to ventricles via atrioventricular node (AVN). To further study the role of resident macrophages in cardiac conduction system, a proper isolation of resident macrophages from SAN and AVN is necessary, but it remains challenging. Read More

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Right atrium and cryptogenic ischaemic stroke in the young: a case-control study.

Open Heart 2021 May;8(1)

Department of Clinical Physiology and Nuclear Medicine, HUS Medical Imaging Center, Helsinki, Finland.

Background: Recent studies suggest left atrial (LA) dysfunction in cryptogenic stroke. We studied the dynamics of right atrium (RA) and right atrial appendage (RAA) in young adults with cryptogenic stroke. We hypothesised that bi-atrial dysfunction and blood stagnation might contribute to thrombosis formation in patients with patent foramen ovale (PFO), as deep venous thrombosis is detected only in the minority of patients. Read More

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Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.

Elife 2021 May 18;10. Epub 2021 May 18.

Department of Biological Structure, University of Washington, Seattle, United States.

This study provides transcriptomic characterization of the cells of the crista ampullaris, sensory structures at the base of the semicircular canals that are critical for vestibular function. We performed single-cell RNA-seq on ampullae microdissected from E16, E18, P3, and P7 mice. Cluster analysis identified the hair cells, support cells and glia of the crista as well as dark cells and other nonsensory epithelial cells of the ampulla, mesenchymal cells, vascular cells, macrophages, and melanocytes. Read More

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The f subunit of human ATP synthase is essential for normal mitochondrial morphology and permeability transition.

Cell Rep 2021 May;35(6):109111

Department of Biomedical Sciences, University of Padova, Padova 35121, Italy; Consiglio Nazionale delle Ricerche Institute of Neuroscience, Padova 35121, Italy; Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna 40126, Italy. Electronic address:

The f subunit is localized at the base of the ATP synthase peripheral stalk. Its function in the human enzyme is poorly characterized. Because full disruption of its ATP5J2 gene with the CRISPR-Cas9 strategy in the HAP1 human model has been shown to cause alterations in the amounts of other ATP synthase subunits, here we investigated the role of the f subunit in HeLa cells by regulating its levels through RNA interference. Read More

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Catheter-related giant right atrial thrombosis mimicking a myxoma: A case report.

Exp Ther Med 2021 Jun 14;21(6):603. Epub 2021 Apr 14.

Department of Cardiovascular Surgery, 'Prof. Dr. C. C.' Iliescu Emergency Institute for Cardiovascular Diseases, 022322 Bucharest, Romania.

Despite the development of imagistic methods, the differential diagnosis of a right atrial mass may be difficult to be established, the most common pathologies which should be taken in consideration being represented by thrombus, tumors, prominent crista terminalis, or vegetation of infectious endocarditis. In this study, we present the case of a 63-year-old man with chronic kidney disease, in hemodialysis (HD) with a silicone central venous catheter (CVC) with the incidental transthoracic echocardiography (transthoracic echocardiogram, TTE) finding of a tumoral mass of 35x26 mm in the right atrium (RA), not related with the catheter, which was diagnosed as right atrial myxoma and underwent surgical excision. After reviewing the histopathology probe, the diagnosis of right atrial thrombus was confirmed. Read More

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Three-dimensional histological explanation of the dermoscopy patterns in acral melanocytic lesions.

J Dermatol 2021 Apr 27. Epub 2021 Apr 27.

Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Dermoscopic images of pigmented lesions have distinct features on the sole where skin ridges and furrows exist. Pigmentation of benign nevus usually locates on the skin furrow, while the malignant melanoma is pigmented on the skin ridge. Correspondence between dermoscopy and pathology in the pigmented lesions on soles have been studied based on conventional vertical pathological images. Read More

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Directed graph mapping exceeds phase mapping in discriminating true and false rotors detected with a basket catheter in a complex in-silico excitation pattern.

Comput Biol Med 2021 Jun 15;133:104381. Epub 2021 Apr 15.

Department of Physics and Astronomy, Ghent University, Ghent, Belgium.

Atrial fibrillation (AF) is the most frequently encountered arrhythmia in clinical practise. One of the major problems in the management of AF is the difficulty in identifying the arrhythmia sources from clinical recordings. That difficulty occurs because it is currently impossible to verify algorithms which determine these sources in clinical data, as high resolution true excitation patterns cannot be recorded in patients. Read More

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Epiphenomenal Re-Entry and Spurious Focal Activation Detection by Atrial Fibrillation Mapping Algorithms.

JACC Clin Electrophysiol 2021 Mar 25. Epub 2021 Mar 25.

Division of Cardiac Electrophysiology, Houston Methodist DeBakey Heart and Vascular Center, Houston, Texas, USA. Electronic address:

Objectives: The purpose of this study was to validate the ability of mapping algorithms to detect rotational activations (RoA) and focal activations (FoA) during fibrillatory conduction (FC) and atrial fibrillation (AF) and understand their mechanistic relevance.

Background: Mapping algorithms have been proposed to detect RoA and FoA to guide AF ablation.

Methods: Rapid left atrial pacing created FC-fibrillatory electrograms-with and without AF induction in dogs (n = 17). Read More

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Evaluation of facial soft tissues by stereophotogrammetry method in patients with obstructive sleep apnoea: a morphological study.

Folia Morphol (Warsz) 2021 29;80(2):432-441. Epub 2021 Mar 29.

Department of Orthodontics, Faculty of Dentistry, Izmir Katip Çelebi University, Izmir, Turkey.

Background: We aimed to use the "SomnoMed MAS" device, which brings the mandible forward in obstructive sleep apnoea syndrome patients due to mandibular retrognathia, and to examine its effects on facial soft tissues by stereophotogrammetry (3dMD) method.

Materials And Methods: Thirty-one patients with a mean age of 44 years and 6 months were included in the study. SomnoMed MAS, one of the splint appliances that position the mandible in front, was applied to all patients and the changes in facial soft tissues were examined by overlapping the images taken at different times with the 3dMD face system. Read More

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Comparative analysis of the chondrocranium and hyobranchial skeleton of bromeliad arboreal frog larvae of the genus Phyllodytes Wagler, 1830 (Anura, Hylidae).

Zootaxa 2020 Dec 15;4895(2):zootaxa.4895.2.4. Epub 2020 Dec 15.

Universidade Federal da Bahia, Programa de Pós-Graduação em Biodiversidade e Evolução (PPGBioEvo-UFBA), Instituto de Biologia, Campus Universitário de Ondina, Rua Barão de Jeremoabo, s/n, Ondina, CEP 40170-115, Salvador, Bahia, Brasil..

The genus Phyllodytes comprises 15 species, ten of them having their tadpole external morphology described in the literature. However, there are few descriptive studies on chondrocranium and hyobranchial skeleton. In this work, we describe the chondrocranium and hyobranchial skeleton of Phyllodytes larvae and discuss shared features and interspecific variation. Read More

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December 2020

Structural and Functional Properties of Subsidiary Atrial Pacemakers in a Goat Model of Sinus Node Disease.

Front Physiol 2021 4;12:592229. Epub 2021 Mar 4.

Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom.

Background: The sinoatrial/sinus node (SAN) is the primary pacemaker of the heart. In humans, SAN is surrounded by the paranodal area (PNA). Although the PNA function remains debated, it is thought to act as a subsidiary atrial pacemaker (SAP) tissue and become the dominant pacemaker in the setting of sinus node disease (SND). Read More

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Dural arteriovenous fistula with crista galli erosion in a patient with suspected sinusitis.

Radiol Case Rep 2021 May 25;16(5):1028-1031. Epub 2021 Feb 25.

Department of Neurological Surgery, Juntendo University Urayasu Hospital, 2-1-1 Tomioka, Urayasu, Chiba, 279-0021, Japan.

Dural arteriovenous fistula (dAVF) of the anterior cranial fossa is an aggressive entity with a high risk of intracranial hemorrhage. A 38-year-old woman presented to our institution with nasal obstruction and discharge. Computed tomography (CT) scans performed for suspected sinusitis revealed erosion of the crista galli predominantly on the left side. Read More

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Ultrastructural Changes of the Mitochondrion During the Life Cycle of Trypanosoma brucei.

J Eukaryot Microbiol 2021 May 19;68(3):e12846. Epub 2021 Mar 19.

Institute of Parasitology, Biology Center, Czech Academy of Sciences & Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.

The mitochondrion is crucial for ATP generation by oxidative phosphorylation, among other processes. Cristae are invaginations of the mitochondrial inner membrane that house nearly all the macromolecular complexes that perform oxidative phosphorylation. The unicellular parasite Trypanosoma brucei undergoes during its life cycle extensive remodeling of its single mitochondrion, which reflects major changes in its energy metabolism. Read More

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Anatomical Variations of the Nasal Cavities and Paranasal Sinuses: A Systematic Review.

Cureus 2021 Jan 15;13(1):e12727. Epub 2021 Jan 15.

Anatomy, Medical School, National and Kapodistrian University of Athens, Athens, GRC.

The anatomy of the nasal cavities and paranasal sinuses is one of the most varied in the human body. The aim of this study is to review the prevalence of anatomical variations in the sinonasal area. This systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Read More

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January 2021

An anatomical approach to determine the location of the sinoatrial node during catheter ablation.

J Cardiovasc Electrophysiol 2021 May 4;32(5):1320-1327. Epub 2021 Mar 4.

Department of Cardiovascular Medicine, National Cerebral and Cardiovascular Center, Suita, Osaka, Japan.

Introduction: The sinoatrial node (SAN) should be identified before superior vena cava (SVC) isolation to avoid SAN injury. However, its location cannot be identified without restoring sinus rhythm. This study evaluated the usefulness of the anatomically defined SAN by comparing it with the electrically confirmed SAN (e-SAN) to predict the top-most position of e-SAN and thus establish a safe and more efficient anatomical reference for SVC isolation than the previously reported reference of the right superior pulmonary vein (RSPV) roof. Read More

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The Radiological Evaluation of the Crista Galli and Its Clinical Implications for Anterior Skull Base Surgery.

J Craniofac Surg 2021 Jan 29. Epub 2021 Jan 29.

Department of Anatomy, Faculty of Medicine Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Gaziantep University, Gaziantep, Turkey.

Introduction: Crista galli is extremely important in the endoscopic transcribriform approaches and some extradural approaches. Because of its clinical significance, the morphometric properties of crista galli should be well known. In this study, it was aimed to examine the morphometry and the pneumatization incidence of the crista galli in cone beam computed tomography (CBCT) images. Read More

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January 2021

Benign Structures Mimicking Right Atrial Masses on Prenatal Ultrasound.

Case Rep Pediatr 2021 12;2021:8889941. Epub 2021 Jan 12.

Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USA.

Advances in imaging have resulted in more frequent reporting of primitive right atrial structures which can sometimes mimic cardiac tumors in prenatal ultrasound. Prominent crista terminalis and Chiari network are examples of these structures. We describe two cases of pregnant women referred to the fetal cardiology clinic for fetal echocardiography for right atrial masses seen on prenatal ultrasound suspicious of tuberous sclerosis. Read More

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January 2021

Tmc Reliance Is Biased by the Hair Cell Subtype and Position Within the Ear.

Front Cell Dev Biol 2020 7;8:570486. Epub 2021 Jan 7.

Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Case Western Reserve University, Cleveland, OH, United States.

Hair cells are heterogenous, enabling varied roles in sensory systems. An emerging hypothesis is that the transmembrane channel-like (Tmc) proteins of the hair cell's mechanotransduction apparatus vary within and between organs to permit encoding of different mechanical stimuli. Five anatomical variables that may coincide with different Tmc use by a hair cell within the ear are the containing organ, cell morphology, cell position within an organ, axis of best sensitivity for the cell, and the hair bundle's orientation within this axis. Read More

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January 2021

BPPV Viewer: A downloadable 3D BPPV model for study of otolith disease.

World J Otorhinolaryngol Head Neck Surg 2021 Jan 8;7(1):34-39. Epub 2020 Jun 8.

Christiana Care Health Systems, Newark, DE, USA.

Objective: To develop a downloadable three-dimensional (3D) study tool of the membranous labyrinth in order to facilitate the study of benign paroxysmal positional vertigo (BPPV).

Background: The diagnosis and treatment of BPPV depend on an understanding of the anatomy of the vestibular labyrinth and its position relative to the head. To date, many illustrations have been made to explain principles of diagnosis and treatment of BPPV, but few have been based on anatomical studies of the membranous labyrinth. Read More

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January 2021

Anthropometric changes in the skull base in children with sagittal craniosynostosis submitted to surgical correction.

Childs Nerv Syst 2021 May 15;37(5):1669-1676. Epub 2021 Jan 15.

Pediatric Neurosurgery, Department of Neurosurgery, São Paulo University Medical School, São Paulo, Brazil.

Purpose: Studies have examined the impact of fusion of the sagittal suture in the skull base while others have evaluated the growth of the skull base before and after surgery. This study aims to perform the anthropometric measures of the skull base in children with scaphocephaly to evaluate the influence of surgical repair in the remodeling of the skull base and anthropometric measures.

Methods: Twenty-one children with diagnosis of scaphocephaly were operated between April 2007 and October 2008, and anthropometric measures at the skull base were performed before and after a year of surgery. Read More

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The Intersection of Cognitive Ability and HIV: A Review of the State of the Nursing Science.

J Assoc Nurses AIDS Care 2021 May-Jun 01;32(3):306-321

Drenna Waldrop, PhD, is a Professor and Assistant Dean for Research Operations & Training, Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, Georgia, USA. Crista Irwin, BSN, RN, is a PhD Student, Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, Georgia, USA. W. Chance Nicholson, PhD, MSN, PMHNP-BC, is a Nurse Practitioner and Assistant Professor, Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, Georgia, USA. Cheryl A. Lee, BSN, RN, is a PhD Student, School of Nursing, University of Alabama at Birmingham, Birmingham, Alabama, USA. Allison Webel, PhD, RN, FAAN, is an Associate Professor, Frances Bolton School of Nursing, Case Western University, Cleveland, Ohio, USA. Pariya L. Fazeli, PhD, is an Associate Professor, School of Nursing, University of Alabama at Birmingham, Birmingham, Alabama, USA. David E. Vance, PhD, MGS, is a Professor, School of Nursing, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Abstract: Neurocognitive problems have been endemic to the HIV epidemic since its beginning. Four decades later, these problems persist, but currently, they are attributed to HIV-induced inflammation, the long-term effects of combination antiretroviral therapy, lifestyle (i.e. Read More

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January 2021

Unexpected larger distribution of paleogene stem-rollers (AVES, CORACII): new evidence from the Eocene of Patagonia, Argentina.

Sci Rep 2021 Jan 14;11(1):1363. Epub 2021 Jan 14.

Department of Geosciences and Earth and Environmental Systems Institute, Pennsylvania State University, University Park, PA, 16802, USA.

Here we present the first record of a stem-Coracii outside the Holarctic region, found in the early Eocene of Patagonia at the Laguna del Hunco locality. Ueekenkcoracias tambussiae gen. et sp. Read More

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January 2021

Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse.

J Vis Exp 2020 12 22(166). Epub 2020 Dec 22.

University Hospital Munich, Department of Medicine I, Ludwig Maximilian University Munich; Walter Brendel Center of Experimental Medicine, Ludwig Maximilian University Munich; German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance.

The electrical signal physiologically generated by pacemaker cells in the sinoatrial node (SAN) is conducted through the conduction system, which includes the atrioventricular node (AVN), to allow excitation and contraction of the whole heart. Any dysfunction of either SAN or AVN results in arrhythmias, indicating their fundamental role in electrophysiology and arrhythmogenesis. Mouse models are widely used in arrhythmia research, but the specific investigation of SAN and AVN remains challenging. Read More

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December 2020

Determinants, and implications, of the shape and size of thylakoids and cristae.

John A Raven

J Plant Physiol 2021 Feb 15;257:153342. Epub 2020 Dec 15.

Division of Plant Science, University of Dundee at the James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK; University of Technology, Sydney, Climate Change Cluster, Faculty of Science, Sydney, Ultimo, NSW, 2007, Australia; School of Biological Sciences, University of Western Australia, Crawley, WA, 6009, Australia. Electronic address:

Thylakoids are flattened sacs isolated from other membranes; cristae are attached to the rest of the inner mitochondrial membrane by the crista junction, but the crista lumen is separated from the intermembrane space. The shape of thylakoids and cristae involves membranes with small (5-30 nm) radii of curvature. While the mechanism of curvature is not entirely clear, it seems to be largely a function of Curt proteins in thylakoids and Mitochondrial Organising Site and Crista Organising Centre proteins and oligomeric FF ATP synthase in cristae. Read More

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February 2021

Consistent removal of hair cells in vestibular end organs by time-dependent transtympanic administration of gentamicin in guinea pigs.

J Neurosci Methods 2021 Mar 7;351:109049. Epub 2021 Jan 7.

Department of Otolaryngology, Head and Neck Surgery, Yamagata University Faculty of Medicine, Yamagata, Japan. Electronic address:

Background: Vestibular hair cell loss and its role in balance disorders are not yet completely understood due largely to the lack of precise hair cell damage protocols.

New Method: Our damage protocol aims to selectively remove type I hair cells in a way that produces consistent and predictable lesions that can be used for reliable inter-animal and inter-group comparison in balance research. This objective is achieved by transtympanic injection of gentamicin on both the round window membrane and oval window over a fixed time period followed by thorough washing. Read More

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Ultrahigh resolution electroanatomical mapping of the transverse conduction of the right atrial posterior wall in cases with and without typical atrial flutter.

J Cardiovasc Electrophysiol 2021 Feb 29;32(2):297-304. Epub 2020 Dec 29.

Department of Cardiovascular Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.

Introduction: The right atrial posterior wall (RAPW) is known to form a conduction barrier during typical atrial flutter (AFL). We evaluated the transverse conduction properties of RAPW in patients with and without typical AFL using an ultrahigh resolution electroanatomical mapping system.

Methods And Results: This study included 41 patients who underwent catheter ablation of AF, typical or atypical AFL, in whom we performed RAPW mapping with an ultrahigh resolution mapping system during typical AFL and coronary sinus ostial pacing with three different pacing cycle lengths (PCLs) (1) PCL1: PCL within 40 ms of the AFL cycle length in patients with typical AFL or 250-300 ms for those without, (2) PCL2: 400 ms, (3) PCL3: PCL just faster than the sinus rate. Read More

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February 2021