7,282 results match your criteria counting kit-8


Circ_0000345 Protects Endothelial Cells From Oxidized Low-Density Lipoprotein-Induced Injury by miR-129-5p/Ten-Eleven Translocation Axis.

J Cardiovasc Pharmacol 2021 May;77(5):603-613

Dartpartment of Cardiovascular Surgery, The Third People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China.

Abstract: Circular RNAs have shown regulatory functions in atherosclerosis (AS) progression. Here, we explored the role and working mechanism of circ_0000345 in the AS cell model in vitro. Quantitative real-time polymerase chain reaction was applied to measure the enrichment of circ_0000345, microRNA-129-5p (miR-129-5p), and ten-eleven translocation-2 (TET2) messenger RNA. Read More

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Circular RNA circ_0010729 Knockdown Attenuates Oxygen-Glucose Deprivation-Induced Human Cardiac Myocytes Injury by miR-338-3p/CALM2 Axis.

J Cardiovasc Pharmacol 2021 May;77(5):594-602

Department of Cardiovascular III, Cangzhou Central Hospital, Cangzhou, Hebei, China.

Abstract: Circular RNAs have pivotal roles in cardiovascular disease. The injury of cardiac myocytes is associated with occurrence of cardiovascular disease. Circular RNA hsa_circ_0010729 (circ_0010729) is associated with cardiac myocytes injury. Read More

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ERK/Drp1-dependent mitochondrial fission contributes to HMGB1-induced autophagy in pulmonary arterial hypertension.

Cell Prolif 2021 May 4:e13048. Epub 2021 May 4.

Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Objectives: High-mobility group box-1 (HMGB1) and aberrant mitochondrial fission mediated by excessive activation of GTPase dynamin-related protein 1 (Drp1) have been found to be elevated in patients with pulmonary arterial hypertension (PAH) and critically implicated in PAH pathogenesis. However, it remains unknown whether Drp1-mediated mitochondrial fission and which downstream targets of mitochondrial fission mediate HMGB1-induced pulmonary arterial smooth muscle cells (PASMCs) proliferation and migration leading to vascular remodelling in PAH. This study aims to address these issues. Read More

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Circ_0017956 promotes the proliferation and metastasis of non-small cell lung cancer through regulating miR-515-5p/ITGB8 axis.

Authors:
Na Li Dan Zhu

Cell Cycle 2021 May 4:1-13. Epub 2021 May 4.

Department of Respiratory, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.

Circular RNAs (circRNAs) have been reported to exert vital roles in the tumorigenesis of non-small cell lung cancer (NSCLC). The study aimed to probe the function of circ_0017956 in NSCLC development. The expression of circ_0017956, microRNA (miR)-515-5p and integrin subunit beta 8 (ITGB8) was gauged by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Read More

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Long non-coding RNA XIST promotes retinoblastoma cell proliferation, migration, and invasion by modulating microRNA-191-5p/brain derived neurotrophic factor.

Bioengineered 2021 Dec;12(1):1587-1598

Department of ophtalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) is oncogenic in multiple cancers. Herein, the present study is aimed at delving into how XIST functions in retinoblastoma (RB) and investigating its underlying mechanism. In this study, XIST, miR-191-5p, BDNF mRNA, and BDNF expression levels in RB tissues or cell lines were examined by quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot. Read More

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December 2021

PLAC1 Regulates the Occurrence of Fetal Growth Restriction by Inhibiting the Apoptosis of Trophoblast Cells.

Ann Clin Lab Sci 2021 Mar;51(2):182-189

Department of Obstetrics, Jiaxing Municipal Maternal and Child Health Care Hospital

Objective: Fetal growth restriction (FGR) refers to impaired and insufficient intrauterine growth potential caused by a variety of adverse factors and is a serious perinatal complication that leads to fetal or neonatal mortality and morbidity. FGR has numerous causes, and its pathogenesis has not been fully understood. Recently, increasing numbers of researchers have begun to focus on the placenta, the only link between the fetus and the mother. Read More

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SQSTM1/p62 Promotes Cell Growth and Triggers Autophagy in Papillary Thyroid Cancer by Regulating the AKT/AMPK/mTOR Signaling Pathway.

Front Oncol 2021 15;11:638701. Epub 2021 Apr 15.

Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Thyroid cancer is one of the most common endocrine malignancies worldwide, and papillary thyroid cancer (PTC) is the most common pathologic type of thyroid cancer. SQSTM1/p62 activity mediates different biological functions. This study aimed to investigate the effect of SQSTM1/p62, a multifunctional receptor, on biological function and autophagy characteristics in the human PTC cell line TPC-1. Read More

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Astragaloside IV Inhibits Galactose-Deficient IgA1 Secretion miR-98-5p in Pediatric IgA Nephropathy.

Front Pharmacol 2021 16;12:658236. Epub 2021 Apr 16.

Department of Pediatrics, The Third Xiangya Hospital, Central South University, Changsha, China.

The factor associated with IgA nephropathy (IgAN) is an abnormality of IgA known as galactose-deficient IgA1 (Gd-IgA1). The purpose of this study was to determine the molecular role played by miRNAs in the formation of Gd-IgA1 in IgAN and investigate the regulatory role of Astragaloside IV (AS-IV) in miRNAs. Bioinformatics analysis, along with functional and mechanistic experiments, were used to investigate the relationship and function of miRNA, β-1, 3-galactosyltransferase (C1GALT1), Gd-IgA1, and AS-IV. Read More

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Upregulation of ubiquitin-conjugating enzyme E2T (UBE2T) predicts poor prognosis and promotes hepatocellular carcinoma progression.

Bioengineered 2021 Dec;12(1):1530-1542

Department of Radiotherapy, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, China.

Reportedly, ubiquitin-conjugating enzyme E2T (UBE2T) is closely related to the progression of several malignancies. This work is aimed to probe the role of UBE2T in the progression of hepatocellular carcinoma (HCC) patients. The microarray analysis was executed to screen the differentially expressed genes (DEGs) in HCC tissues. Read More

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December 2021

LncRNA NKILA knockdown promotes cell viability and represses cell apoptosis, autophagy and inflammation in lipopolysaccharide-induced sepsis model by regulating miR-140-5p/CLDN2 axis.

Biochem Biophys Res Commun 2021 Apr 28;559:8-14. Epub 2021 Apr 28.

Department of Emergency Internal Medicine, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai City, China. Electronic address:

Background: Long non-coding RNAs (lncRNAs) play vital roles in human diseases, including sepsis-induced acute kidney injury (AKI). Here, we aimed to investigate the functions of lncRNA NKILA in sepsis-engendered AKI.

Methods: HK2 cells stimulated with LPS were used to mimic sepsis-induced AKI in vitro. Read More

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Actein antagonizes colorectal cancer through blocking PI3K/Akt pathways by downregulating IMPDH2.

Anticancer Drugs 2021 Apr 23. Epub 2021 Apr 23.

Anorectal Department, Affiliated Hospital of Shandong University of Traditional Chinese Medicine College of Health, Shandong University of Traditional Chinese Medicine Preventive Medicine Department, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.

Background: Actein, a triterpene glycoside, isolated from rhizomes of Cimicifuga foetida, was reported to exhibit anticancer effects in vitro and in vivo. However, the effects of actein on colorectal cancer (CRC) remains unclear. As one of the most popular cancers all over the world, CRC ranked third place in both men and women. Read More

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Hsa_circ_0069094 knockdown inhibits cell proliferation, migration, invasion and glycolysis, while induces cell apoptosis by miR-661/HMGA1 axis in breast cancer.

Anticancer Drugs 2021 Apr 23. Epub 2021 Apr 23.

Department of Oncology, Weihai Central Hospital Department of Oncology, Shidao People's Hospital Of Rongcheng, Weihai, Shandong Department of Thyroid and Breast Surgery, The Second People's Hospital of Lianyungang Department of Thoracic Surgery, The Second People's Hospital of Lianyungang, Lianyungang, Jiangsu, China.

Circular RNAs (circRNAs) are revealed to regulate breast cancer progression. This study aimed to investigate hsa_circ_0069094-mediated effects on breast cancer cell malignancy. Quantitative real time PCR was employed to evaluate the expressions of hsa_circ_0069094, miR-661 and high mobility group A1 (HMGA1). Read More

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CircRASSF2 facilitates the proliferation and metastasis of colorectal cancer by mediating the activity of Wnt/β-catenin signaling pathway by regulating the miR-195-5p/FZD4 axis.

Anticancer Drugs 2021 Apr 23. Epub 2021 Apr 23.

Department of Gastrointestinal Surgery, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai City, Zhejiang Province, China.

Circular RNAs (circRNA) are a key regulator of cancer progression, including colorectal cancer (CRC). Nevertheless, the role of circRASSF2 in CRC remains unclear. Quantitative real-time PCR was used to measure the expression of circRASSF2 and miR-195-5p. Read More

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Silencing of circ_0078607 prevents development of gastric cancer and inactivates the ERK1/2/AKT pathway through the miR-188-3p/RAP1B axis.

Anticancer Drugs 2021 Apr 23. Epub 2021 Apr 23.

Department of Oncology, Heilongjiang Province Hospital Department of Hematology, Harbin Medical University Cancer Hospital Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.

Aim: The aim of this study is to explore the expression and mechanism of circ_0078607 on proliferation and apoptosis of gastric cancer.

Methods: Real time PCR (RT-PCR) was performed to detect the expression of circ_0078607 in gastric cancer tumor tissues, plasma and cell lines. Cell viability was detected by cell counting Kit-8. Read More

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LncRNA MIR503HG regulated cell viability, metastasis and apoptosis of cervical cancer via miR-191/CEBPB axis.

Eur Rev Med Pharmacol Sci 2021 Apr;25(8):3200-3210

Department of Mother and Baby Health Paradise, People's Hospital of Rizhao, Rizhao, China.

Objective:   The long non-coding RNA MIR503 host gene (MIR503HG) plays a role in suppressing or promoting cancer in many types of human malignant tumors. The role of MIR503HG in cervical cancer is still unknown.

Patients And Methods: The expression level of MIR503HG in cervical cancer tissues and cell lines was accessed using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. Read More

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Autoantibody against angiotensin II type I receptor induces pancreatic β-cell apoptosis via enhancing autophagy.

Acta Biochim Biophys Sin (Shanghai) 2021 Apr 30. Epub 2021 Apr 30.

Key Laboratory of Cellular Physiology (Shanxi Medical University), Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan 030001, China.

Autoantibody against the angiotensin II type I receptor (AT1-AA) has been found in the serum of patients with diabetes mellitus (DM). However, it remains unclear whether AT1-AA induces β-cell apoptosis and participates in the development of DM. In this study, an AT1-AA-positive rat model was set up by active immunization, and AT1-AA IgG was purified. Read More

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Erythropoietin Attenuates Experimental Contrast-Induced Nephrology: A Role for the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Signaling Pathway.

Front Med (Lausanne) 2021 13;8:634882. Epub 2021 Apr 13.

Division of Nephrology, Department of Medicine, West China Hospital of Sichuan University, Chengdu, China.

The aim of the present study was to investigate the effect of erythropoietin (EPO) on contrast-induced nephrology (CIN) and . Male C57BL/6J mice were divided into four groups: control, CIN (iohexol 6.0 g/kg), EPO (3,000 IU/kg), and CIN+EPO. Read More

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Da Cheng Qi Decoction Alleviates Cerulein-Stimulated AR42J Pancreatic Acinar Cell Injury via the JAK2/STAT3 Signaling Pathway.

Evid Based Complement Alternat Med 2021 9;2021:6657036. Epub 2021 Apr 9.

Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Background: Acute pancreatitis (AP) is a common acute abdomen inflammation, characterized by the dysregulation of digestive enzyme production and secretion. Many studies have shown that Da Cheng Qi Decoction (DCQD) is a secure, effective prescription on AP. In this study, cerulein-stimulated AR42J cells damage model was established to further explore the feasibility and underlying mechanism of DCQD as a potential inhibitor of JAK2/STAT3 pathway for the treatment of AP. Read More

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Long non-coding RNA AFAP1-AS1 facilitates ovarian cancer progression by regulating the miR-107/PDK4 axis.

J Ovarian Res 2021 Apr 29;14(1):60. Epub 2021 Apr 29.

Chongqing Health Center for Women and Children, Chongqing, 401147, China.

Background: Abnormally expressed in various tumors, long non-coding RNAs (lncRNAs) feature prominently in tumor development, yet little is still known regarding the functional roles of lncRNA AFAP1 antisense RNA 1 (AFAP1-AS1) in ovarian cancer (OC).

Methods: The relative expression levels of lncRNA AFAP1-AS1, microRNA (miR)-107 and pyruvate dehydrogenase kinase isozyme 4 (PDK4) mRNA were assessed by quantitative real-time PCR. PDK4, PCNA and cyclin D1 expression levels were determined using Western blot analysis. Read More

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Impact of Implant Surface Material and Microscale Roughness on the Initial Attachment and Proliferation of Primary Human Gingival Fibroblasts.

Biology (Basel) 2021 Apr 22;10(5). Epub 2021 Apr 22.

Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria.

Due to the rising demand for zirconia (Zr) based implant systems, it is important to understand the impact of Zr and titanium (Ti) implants and particularly their topography on soft tissue healing. As human gingival fibroblasts (hGFs) are the predominant cells in peri-implant soft tissue, we focused on examining the effect of implant material and surface roughness on hGFs' initial attachment, growth and the expression of proteins involved in the focal adhesion. hGFs isolated from eight healthy donors were cultured on the following surfaces: smooth titanium machined surface (TiM), smooth zirconia machined surface (ZrM), moderately rough titanium surface (SLA), or moderately rough zirconia surface (ZLA) for up to 14 days. Read More

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Germinated Fermented with SC65 Reduces Particulate Matter Induced Type II Alveolar Epithelial Apoptotic Cell Death.

Int J Mol Sci 2021 Apr 1;22(7). Epub 2021 Apr 1.

Department of Food Science and Biotechnology, College of BioNano, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 461-701, Gyeonggi-do, Korea.

Particulate matter (PM) is a significant environmental pollutant that promotes respiratory diseases, including lung injury and inflammation, by inducing oxidative stress. (black soybean) is traditionally used to prevent chronic respiratory disease via inducing antioxidant and anti-inflammatory effects. To investigate the effects of SC65 fermented GR (GR-SC65) and ON81A (GR-ON81A) against PM-induced oxidative stress and cell death in A549 cells, we performed the 2-7-dichlorodihydrofluorescein diacetate and cell counting kit-8 assays, as well as Hoechst 33342 and propidium iodide staining and western blotting. Read More

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Phenalen-1-one-mediated photodynamic therapy inhibits keloid graft progression by reducing vessel formation and promoting fibroblast apoptosis.

Adv Clin Exp Med 2021 Apr;30(4):431-439

Department of Burn Orthopedics, Xingtai People's Hospital, China.

Background: Keloid is a unique refractory syndrome characterized by a proliferation disorder of the fibroblasts. Recently, photodynamic therapy (PDT) has become a promising technique to modulate fibroblasts. However, use of the photosensitizer Phenalen-1-one (Ph1) in PDT for keloid remains to be explored. Read More

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Eplerenone inhibits oxidized low-density lipoprotein-induced proliferation and migration of vascular smooth muscle cells by downregulating GPER expression.

Adv Clin Exp Med 2021 Apr;30(4):405-412

Nursing Department, Weihai Central Hospital, China.

Background: Excessive proliferation and migration of vascular smooth muscle cells (VSMCs) are associated with the pathogenesis of atherosclerosis (AS). Eplerenone (EPL), a novel selective aldosterone receptor blocker, plays a substantial role in the treatment of cardiovascular disease. The G protein-coupled estrogen receptor (GPER) is a target of EPL as the STITCH website predicated. Read More

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Sulfasalazine maintains blood-brain barrier integrity and relieves lipopolysaccharide-induced inflammation in hCMEC/D3 cells.

Neuroreport 2021 May;32(8):672-677

Department of Orthopedics, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning Province, China.

Sulfasalazine is a recognized therapy for inflammatory bowel disease and is of paramount importance for maintaining intestinal barrier homeostasis. However, its effects on blood-brain barrier (BBB) function and inflammation have not yet been explored. We sought to examine whether sulfasalazine has anti-inflammatory and antiapoptotic effects on the BBB. Read More

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Resveratrol protects intestinal epithelial cells against radiation-induced damage by promoting autophagy and inhibiting apoptosis through SIRT1 activation.

J Radiat Res 2021 Apr 29. Epub 2021 Apr 29.

Tianjin Union Medical Center of Nankai University, Department of Oncology, Tianjin 300121, China.

Intrinsic autophagy is important for the maintenance of intestinal homeostasis and intestinal regeneration. Ionizing radiation suppresses intrinsic autophagy and reduces damage-induced regeneration in the intestine, resulting in intestinal injury. Resveratrol, a sirtuin 1 (SIRT1) agonist, promotes autophagy and exerts radioprotective effect. Read More

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The Clinical Significance and Potential Role of Cathepsin S in IgA Nephropathy.

Front Pediatr 2021 12;9:631473. Epub 2021 Apr 12.

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, China.

Cathepsin S (CTSS) is an important lysosomal cysteine protease. This study aimed at investigating the clinical significance of CTSS and underlying mechanism in immunoglobulin A nephropathy (IgAN). This study recruited 25 children with IgAN and age-matched controls and their serum CTSS levels were measured by enzyme-linked immunosorbent assay (ELISA). Read More

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Rapamycin Antagonizes BCRP-Mediated Drug Resistance Through the PI3K/Akt/mTOR Signaling Pathway in mPRα-Positive Breast Cancer.

Front Oncol 2021 12;11:608570. Epub 2021 Apr 12.

Department of Pathology, School of Basic Medical Sciences, Shandong University, Jinan, China.

Purpose: Overexpression of breast cancer (BCa) resistance protein (BCRP) is detected in approximately 30% of BCa cases. BCRP indicates a poor response to chemotherapy, and it has become a classic target to overcome drug-resistant tumor cells. In this study, we aimed to explore the mechanism of BCRP overexpression and a strategy to reverse this overexpression in invasive BCa. Read More

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Long Noncoding RNA SNHG1 Knockdown Ameliorates Apoptosis, Oxidative Stress and Inflammation in Models of Parkinson's Disease by Inhibiting the miR-125b-5p/MAPK1 Axis.

Neuropsychiatr Dis Treat 2021 22;17:1153-1163. Epub 2021 Apr 22.

Department of Neurology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi City, Hubei Province, People's Republic of China.

Background: Parkinson's disease (PD) is a prevalent neurodegenerative disease. Long noncoding RNA small molecule RNA host gene 1 (SNHG1) has been reported to play critical roles in Parkinson's disease (PD) progression. The study aimed to further elucidate the mechanism of SNHG1 in PD pathogenesis. Read More

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STAT3 phosphorylation is required for the HepaCAM-mediated inhibition of castration-resistant prostate cancer cell viability and metastasis.

Prostate 2021 Apr 28. Epub 2021 Apr 28.

School of Clinical Medicine, Chongqing Medical and Pharmaceutical College, Chongqing, China.

Background: Castration-resistant prostate cancer (CRPC) is an advanced disease that is difficult to treat, the mechanism of it is unclear. This study illustrated the function of hepatocyte cell adhesion molecule (HepaCAM) on CRPC cell viability and metastasis.

Methods: The expression of HepaCAM and p-STAT3 in CRPC tissues were determined by immunohistochemistry and western blot analysis. Read More

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Atorvastatin rescues pulmonary artery hypertension via inhibiting the AKT/ERK-dependent PDGF-BB/HIF-1α axis.

Panminerva Med 2021 Apr 28. Epub 2021 Apr 28.

Department of Pathology, Southwest Hospital, Chongqing, China -

Background: To explore the role of Atorvastatin in rescuing pulmonary artery hypertension (PAH) via inhibiting the AKT/ERK-dependent PDGF-BB/HIF-1α axis.

Methods: PAH model in rats was established by MCT induction, followed by Atorvastatin intervention. Pulmonary hemodynamic measurement and pulmonary morphological evaluation in rats were conducted. Read More

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