91 results match your criteria cortex dexamethasone-treated

3D RNA-seq: a powerful and flexible tool for rapid and accurate differential expression and alternative splicing analysis of RNA-seq data for biologists.

RNA Biol 2020 Dec 19:1-14. Epub 2020 Dec 19.

Information and Computational Sciences, The James Hutton Institute, Dundee, UK.

RNA-sequencing (RNA-seq) analysis of gene expression and alternative splicing should be routine and robust but is often a bottleneck for biologists because of different and complex analysis programs and reliance on specialized bioinformatics skills. We have developed the '3D RNA-seq' App, an R shiny App and web-based pipeline for the comprehensive analysis of RNA-seq data from any organism. It represents an easy-to-use, flexible and powerful tool for analysis of both gene and transcript-level gene expression to identify differential gene/transcript expression, differential alternative splicing and differential transcript usage (3D) as well as isoform switching from RNA-seq data. Read More

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December 2020

Analysis of the impact of corticosteroids adjuvant treatment during experimental invasive meningococcal infection in mice.

Steroids 2018 08 11;136:32-39. Epub 2018 May 11.

Invasive Bacterial Infection Unit, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, France. Electronic address:

Invasive meningococcal disease (IMD) is usually associated with intense inflammatory response that is correlated with severe infection. Corticosteroids may regulate this inflammatory response through an early but transient induction of IL-10 that is suggested to improve the outcome of IMD. We explored the mechanism of action of corticosteroids as an adjuvant treatment to antibiotics. Read More

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DHEA supplementation to dexamethasone-treated rabbits alleviates oxidative stress in kidney-cortex and attenuates albuminuria.

J Steroid Biochem Mol Biol 2017 11 4;174:17-26. Epub 2017 Aug 4.

Department of Metabolic Regulation, Institute of Biochemistry, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, Poland. Electronic address:

Our recent study has shown that dehydroepiandrosterone (DHEA) administered to rabbits partially ameliorated several dexamethasone (dexP) effects on hepatic and renal gluconeogenesis, insulin resistance and plasma lipid disorders. In the current investigation, we present the data on DHEA protective action against dexP-induced oxidative stress and albuminuria in rabbits. Four groups of adult male rabbits were used in the in vivo experiment: (1) control, (2) dexP-treated, (3) DHEA-treated and (4) both dexP- and DHEA-treated. Read More

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November 2017

Dexamethasone Alters the Appetite Regulation via Induction of Hypothalamic Insulin Resistance in Rat Brain.

Mol Neurobiol 2017 11 7;54(9):7483-7496. Epub 2016 Nov 7.

Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India.

Elevated levels of glucocorticoid, a steroid hormone released in response to stress, have been implicated in the pathophysiology of diabetes, which is now known to extend its effect on brain functions. Hence, we aimed to investigate the status of brain insulin signaling in response to dexamethasone (a synthetic glucocorticoid) treatment in female Charles Foster rat. This model exhibited pronounced hyperinsulinemia and glucose intolerance with loss in appetite and body weight. Read More

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November 2017

DHEA-induced modulation of renal gluconeogenesis, insulin sensitivity and plasma lipid profile in the control- and dexamethasone-treated rabbits. Metabolic studies.

Biochimie 2016 Feb 23;121:87-101. Epub 2015 Nov 23.

Department of Metabolic Regulation, Institute of Biochemistry, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, Poland.

In view of antidiabetic and antiglucocorticoid effects of dehydroepiandrosterone (DHEA) both in vitro and in vivo studies were undertaken: (i) to elucidate the mechanism of action of both dexamethasone phosphate (dexP) and DHEA on glucose synthesis in primary cultured rabbit kidney-cortex tubules and (ii) to investigate the influence of DHEA on glucose synthesis, insulin sensitivity and plasma lipid profile in the control- and dexP-treated rabbits. Data show, that in cultured kidney-cortex tubules dexP significantly stimulated gluconeogenesis by increasing flux through fructose-1,6-bisphosphatase (FBPase). DexP-induced effects were dependent only upon glucocorticoid receptor. Read More

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February 2016

Neuronal Fos-like immunoreactivity associated with dexamethasone-induced hypertension in rats and effects of glucagon-like peptide-2.

Life Sci 2013 Nov;93(23):889-96

Aims: Dexamethasone-induced hypertension models have been used to study the mechanisms of glucocorticoid induced hypertension, but the role of glucocorticoids in central cardiovascular regulation is not clearly understood. In the present study, we investigated the sites associated with dexamethasone-induced hypertension in the central nervous system in rats. We further investigated whether glucagon-like peptide-2 (GLP-2) was effective for dexamethasone-induced hypertension. Read More

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November 2013

Dexamethasone exacerbates cerebral edema and brain injury following lithium-pilocarpine induced status epilepticus.

Neurobiol Dis 2014 Mar 12;63:229-36. Epub 2013 Dec 12.

Department of Neurological Sciences, College of Medicine, University of Vermont, Burlington 05405, VT, USA; UCL Institute of Child Health, University College London, London, UK.

Anti-inflammatory therapies are the current most plausible drug candidates for anti-epileptogenesis and neuroprotection following prolonged seizures. Given that vasogenic edema is widely considered to be detrimental for outcome following status epilepticus, the anti-inflammatory agent dexamethasone is sometimes used in clinic for alleviating cerebral edema. In this study we perform longitudinal magnetic resonance imaging in order to assess the contribution of dexamethasone on cerebral edema and subsequent neuroprotection following status epilepticus. Read More

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Dexamethasone induces apoptosis of progenitor cells in the subventricular zone and dentate gyrus of developing rat brain.

J Neurosci Res 2013 Sep 17;91(9):1191-202. Epub 2013 May 17.

Division of Newborn Medicine, Department of Pediatrics, University of Mississippi Medical Center, Jackson, Mississippi, USA.

The use of dexamethasone in premature infants to prevent and/or treat bronchopulmonary dysplasia adversely affects neurocognitive development and is associated with cerebral palsy. The underlying mechanisms of these effects are multifactorial and likely include apoptosis. The objective of this study was to confirm whether dexamethasone causes apoptosis in different regions of the developing rat brain. Read More

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September 2013

Stress hormone leads to memory deficits and altered tau phosphorylation in a model of Alzheimer's disease.

J Alzheimers Dis 2012 ;31(1):167-76

Center for Translational Medicine, Temple University, School of Medicine, Philadelphia, PA 19140, USA.

Several studies have linked stress with Alzheimer's disease (AD) vulnerability; however, the mechanism remains to be fully elucidated. In the current paper, we investigated the role of glucocortitcoids on the AD-like phenotype. We administered the glucocorticoid dexamethasone to Tg2576 mice for 4 weeks and then investigated its effect on memory, amyloid-β and tau levels, and metabolism. Read More

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December 2012

Dexamethasone acutely accelerates pleural fluid absorption in mice hydrothoraces.

J Physiol Sci 2010 Jul 10;60(4):299-302. Epub 2010 Apr 10.

Department of Physiology, Medical School, University of Thessaly, and Department of Respiratory Medicine, University Hospital of Larissa, Mezourlo Hill, 41110, Larissa, Greece.

This study assessed the effect of corticosteroid treatment in the clearance of hydrothoraces in mice. Twenty-four C57BL/6 mice were divided into four groups and were injected intrapleurally with 500 microL sterilized PBS-BSA 1% to create isosmotic hydrothoraces. Two groups served as control and two groups were treated with dexamethasone. Read More

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Induction of donor-specific T-cell hyporesponsiveness using dexamethasone-treated dendritic cells in two fully mismatched rat kidney transplantation models.

Transplantation 2008 Nov;86(9):1275-82

Department Nephrology, Leiden University Medical Center, Leiden, The Netherlands.

Background: Dendritic cells (DC) can exert powerful immune stimulatory as well as regulatory functions and are therefore important tools for therapeutic strategies. Dexamethasone (Dex) was previously shown to inhibit DC maturation and to induce regulatory properties both in vitro and in vivo. Here, we investigated the immunoregulatory role of DexDC in two different rat acute rejection models of kidney transplantation. Read More

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November 2008

Effects of round window dexamethasone on residual hearing in a Guinea pig model of cochlear implantation.

Audiol Neurootol 2008 29;13(2):86-96. Epub 2007 Nov 29.

Department of Otolaryngology, University of Melbourne, Parkville, Australia.

Unlabelled: To study electric acoustic stimulation, we have developed a model of guinea pig cochlear implantation via a cochleostomy. Thirty minutes prior to implantation, a hyaluronic acid/carboxymethylcellulose bead, loaded with either dexamethasone or normal saline, was placed upon the round window membrane. Animals that did not receive beads acted as controls. Read More

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February 2008

A segment of gamma ENaC mediates elastase activation of Na+ transport.

J Gen Physiol 2007 Dec 12;130(6):611-29. Epub 2007 Nov 12.

Department of Cell Biology and Physiology, University of Pittsburgh, PA 15261, USA.

The epithelial Na(+) channel (ENaC) that mediates regulated Na(+) reabsorption by epithelial cells in the kidney and lungs can be activated by endogenous proteases such as channel activating protease 1 and exogenous proteases such as trypsin and neutrophil elastase (NE). The mechanism by which exogenous proteases activate the channel is unknown. To test the hypothesis that residues on ENaC mediate protease-dependent channel activation wild-type and mutant ENaC were stably expressed in the FRT epithelial cell line using a tripromoter human ENaC construct, and protease-induced short-circuit current activation was measured in aprotinin-treated cells. Read More

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December 2007

Detection of apoptotic cell death in the thymus of dexamethasone treated rats using [123I]annexin V and in situ oligonucleotide ligation.

J Mol Histol 2007 Aug 29;38(4):313-9. Epub 2007 Jun 29.

Radiopharmaceutical Research Institute, Australian Nuclear Science and Technology Organisation, PMB 1, Menai, Sydney, NSW 2234, Australia.

In the present study we aimed to establish an animal model of dexamethasone (DEX)-induced apoptosis in the thymus of rats. The degree of apoptosis was determined in the same animals at 6 and 11 h after a single administration of DEX (5 mg/kg, ip) by (a) in vivo biodistribution of the uptake of [(123)I]Annexin V, a biomarker of the early stages of apoptosis; (b) in vitro evaluation of the apoptotic index (percentage of number of apoptotic cells versus total number of cells) in the form of DNA fragmentation, on tissue sections using in situ oligo ligation (ISOL). ISOL demonstrated a 62- and 90-fold increase of apoptotic index at 6 and 11 h after DEX administration respectively, in the outer part of the thymic lobule (cortex) and a 25- and 54-fold increases in the inner part of the thymic lobule (medulla) in the corresponding treatment groups. Read More

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Increased extracellular signal regulated kinases phosphorylation in the adrenal gland in response to chronic ACTH treatment.

J Endocrinol 2007 Mar;192(3):647-58

Laboratory of Molecular Cell Biology, Faculty of Medicine of Porto, Porto, Portugal.

ACTH released from the pituitary acts through activation of cAMP/PKA in adrenocortical cells stimulating steroidogenesis. Although ACTH was originally thought to have anti-proliferative effects on the adrenal, recently it has been described that it could also have proliferative effects acting through other signalling cascades. This is also relevant in humans given the increased levels of ACTH occurring together with adrenal cortex hyperplasia observed in Cushing's disease and possibly in other situations such as chronic stress. Read More

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Corticosteroids for treating hypotension in preterm infants.

Cochrane Database Syst Rev 2007 Jan 24(1):CD003662. Epub 2007 Jan 24.

Liverpool Women's Hospital, Neonatal Intensive Care Unit, Crown Street, Liverpool, UK, L 8 7SS.

Background: Systemic hypotension is a relatively common complication of preterm birth and is associated with periventricular haemmorhage, periventricular white matter injury and adverse neurodevelopmental outcome. Corticosteroid treatment has been used as an alternative, or an adjunct, to conventional treatment with volume expansion and vasopressor/inotropic therapy.

Objectives: To determine the effectiveness and safety of corticosteroids used either as primary treatment of hypotension or for the treatment of refractory hypotension in preterm infants. Read More

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January 2007

Prenatal glucocorticoid exposure affects learning and vulnerability of cholinergic neurons.

Neurobiol Aging 2007 Jan 6;28(1):112-21. Epub 2006 Jan 6.

Department of Veterinary Morphophysiology and Animal Production, University of Bologna, 40064 Ozzano Emilia Bologna, Italy.

Prenatal treatment with synthetic glucocorticoids is commonly used as a treatment for women at risk of preterm delivery. However, little is known about the life-long consequences of these treatments on the fetus. In the present study, we evaluated cognitive function as well as susceptibility of cholinergic neurons to (192)IgG-saporin immunolesion in adult rats after prenatal glucocorticoid treatment. Read More

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January 2007

Prenatal programming of adult blood pressure: role of maternal corticosteroids.

Am J Physiol Regul Integr Comp Physiol 2005 Oct 2;289(4):R955-62. Epub 2005 Jun 2.

Division of Nephrology and Hypertension, L463, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Rd., Portland, OR 97239-3098, USA.

Both maternal glucocorticoid administration and maternal dietary protein or food restriction in pregnancy cause fewer nephrons and hypertension in the adult offspring. The purpose of these studies was to determine the extent to which nutritional factors contribute to programming of offspring hypertension by maternal glucocorticoids. Pregnant rats were treated with dexamethasone (100 microg x kg(-1) x d(-1) sc) on days 1-10 (ED) or days 15-20 (LD) of pregnancy. Read More

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October 2005

Differential profile of Nix upregulation and translocation during hypoxia/ischaemia in vivo versus in vitro.

J Cereb Blood Flow Metab 2005 Oct;25(10):1356-65

Fujisawa Institute of Neuroscience in Edinburgh, University of Edinburgh, Edinburgh, UK.

Nix, a hypoxia-sensitive member of the Bcl-2 family, is upregulated at the mRNA level during hypoxia through induction of a hypoxia-inducible factor-1 alpha (HIF-1 alpha) response element in its promoter sequence. However, the mechanism(s) regulating Nix protein activation remain unclear. The present studies examine Nix protein expression and subcellular distribution in response to hypoxic stimuli in vivo and in culture and to two disparate apoptotic stimuli in vitro. Read More

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October 2005

Effects of high altitude exposure on cerebral hemodynamics in normal subjects.

Stroke 2005 Mar 3;36(3):557-60. Epub 2005 Feb 3.

Laboratory of Physiology, Faculty of Medicine, Free University of Brussels, Belgium.

Background And Purpose: Acute mountain sickness (AMS) may be an early stage of high altitude cerebral edema. If so, AMS could result from an alteration of dynamic autoregulation of cerebral blood flow resulting in overperfusion of capillaries and vasogenic cerebral edema.

Methods: We measured middle cerebral artery blood flow velocity (Vmca) by transcranial Doppler and arterial blood pressure by finger plethysmography at 490 m and 20 hours after arrival at 4559 m in 35 volunteers who had been randomized to tadalafil, dexamethasone, or placebo in a study on the pharmacological prevention of high altitude pulmonary edema. Read More

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Glucocorticoids increase osmotic water permeability (Pf) of neonatal rabbit renal brush border membrane vesicles.

Am J Physiol Regul Integr Comp Physiol 2005 May 20;288(5):R1417-21. Epub 2005 Jan 20.

Dept. of Pediatrics, U. T. Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9063, USA.

During postnatal maturation, there is an increase in renal brush border membrane vesicle (BBMV) osmotic water permeability and a parallel increase in aquaporin-1 (AQP1) protein abundance. The mechanisms responsible for these changes remain unknown. Because serum glucocorticoid levels rise postnatally and have previously been linked to other maturational changes in renal function, we examined the effects of glucocorticoids on osmotic (Pf) and diffusional (P(DW)) water permeability and AQP1 protein abundance of renal BBMV. Read More

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Evaluation of dexamethasone for the treatment of intracerebral hemorrhage using a collagenase-induced intracerebral hematoma model in rats.

J Vet Pharmacol Ther 2004 Oct;27(5):321-8

Department of Veterinary Biomedicine, Faculty of Veterinary Medicine, University of Montreal, C.P. 5000, St-Hyacinthe, QC, Canada J2S 7C6.

Dexamethasone was evaluated for the treatment of intracerebral hemorrhage using a rat model of cerebral hematoma induced by intracerebral injection of collagenase. The treatment group consisted of hematoma rats receiving dexamethasone 1 mg/kg intraperitoneal (i.p. Read More

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October 2004

Effects of multiple courses of antenatal corticosteroids on regional brain and somatic tissue water content in ovine fetuses.

J Soc Gynecol Investig 2004 Apr;11(3):166-74

Department of Pediatrics, Brown University Medical School, Women and Infants' Hospital of Rhode Island, Providence 02905-2499, USA.

Objective: To study the effects of single and multiple courses of antenatal corticosteroids on tissue water content in ovine fetuses.

Methods: After chronic catheterization of the ewes and fetuses, the ewes were randomly assigned to receive single or multiple courses of dexamethasone or placebo beginning at 104-106 or 76-78 days' gestation, respectively. In the single course groups, the ewes received dexamethasone (6 mg, n = 6) or placebo (n = 6) as four intramuscular injections every 12 hours over 48 hours. Read More

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Effects of Inula racemosa root and Gymnema sylvestre leaf extracts in the regulation of corticosteroid induced diabetes mellitus: involvement of thyroid hormones.

S Gholap A Kar

Pharmazie 2003 Jun;58(6):413-5

Thyroid Research Unit, School of Life Sciences, D. A. University, Khandwa Road, Indore, India.

The efficacy of Inula racemosa (root) and Gymnema sylvestre (leaf) extracts either alone or in combination was evaluated in the amelioration of corticosteroid-induced hyperglycaemia in mice. Simultaneously thyroid hormone levels were estimated by radio-immunoassay (RIA) in order to ascertain whether the effects are mediated through thyroid hormones or not. While the corticosteroid (dexamethasone) administration increased the serum glucose concentration, it decreased serum concentrations of the thyroid hormones, thyroxine (T4) and triiodothyronine (T3). Read More

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Effects of antenatal corticosteroids on regional brain and non-neural tissue water content in the ovine fetus.

J Soc Gynecol Investig 2003 Feb;10(2):59-66

Department of Pediatrics, Brown Medical School, Women and Infants' Hospital of Rhode Island, Providence, Rhode Island 02905-2499, USA.

Objective: We examined the effects of maternal corticosteroid administration on water content in regional tissue in ovine fetuses at 60%, 80%, and 90% of gestation.

Methods: After catheters were placed in the fetuses, the ewes were given four 6-mg doses of dexamethasone or placebo injections 12 hours apart over 48 hours. Water content of fetal tissue was determined 18 hours after the last injection was given to the ewes. Read More

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February 2003

Selective suppression of renal Na+/H+ exchanger isoform-3 by prolonged stimulation of rats with adrenocorticotropic hormone.

I Khan B Cheng

Endocrine 2001 Dec;16(3):189-94

Department of Biochemistry, Faculty of Medicine, Kuwait University.

Prolonged stimulation with adrenocorticotropic hormone (ACTH) causes hypertension and increases Na+ intake and urine output in humans and animals. However, its biochemical basis remains to be established. Since renal Na+/H+ exchanger isoforms (NHE) and the sodium pump play an important role in this condition, their levels were examined in rats stimulated with ACTH. Read More

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December 2001

Effect of glucocorticoid dexamethasone on cyclic AMP formation stimulated by pituitary adenylate cyclase-activating polypeptide (PACAP) in the cerebral cortex and hypothalamus of chick.

Pol J Pharmacol 2001 Jan-Feb;53(1):39-45

Department of Pharmacodynamics, Medical University of Lódź, Poland.

In this study we tested in chicks the effects of acute and chronic in vivo treatment with a glucocorticoid dexamethasone (4 mg/kg, i.p.) on PACAP-stimulated cyclic AMP formation in [3H]adenine-prelabeled slices of the hypothalamus and cerebral cortex. Read More

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The human homolog of Diminuto/Dwarf1 gene (hDiminuto): a novel ACTH-responsive gene overexpressed in benign cortisol-producing adrenocortical adenomas.

J Clin Endocrinol Metab 2001 Nov;86(11):5130-7

Department of Endocrinology and Metabolism, and Genetics Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.

To elucidate the molecular mechanism of the pathogenesis of benign functioning adrenocortical adenomas causing Cushing's syndrome, we employed suppression PCR-based cDNA subtractive hybridization to identify novel genes that are differentially expressed in the adenoma. In this report we describe the adenoma-specific overexpression of the human homolog of the Diminuto/Dwarf1 (hDiminuto) gene. Northern blot analysis revealed that hDiminuto mRNA was overexpressed in the adenoma tissue of 14 patients with Cushing's syndrome in comparison to the adjacent nontumorous adrenal gland. Read More

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November 2001

Dexamethasone enhances phospholipase D activity in M-1 cells.

Exp Mol Med 2000 Sep;32(3):170-7

Department of Biochemistry, Dong-A University College of Medicine, Pusan, Korea.

Phospholipase D (PLD) is an enzyme involved in signal transduction and widely distributed in mammalian cells. The signal transduction pathways and role for phospholipid metabolism during hormonal response in cortical collecting duct remain partly undefined. It has been reported that dexamethasone increases transepithelial transport in M-1 cells that are derived from the mouse cortical collecting duct. Read More

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September 2000

Chronic electroconvulsive shock decreases (+/-) 1-(4-iodo-2,5-dimethoxyphenyl)-2-aminopropane hydrochloride (DOI)-induced wet-dog shake behaviors of dexamethasone-treated rats.

Life Sci 2000 Feb;66(13):1271-9

Department of Psychiatry and Neurosciences, Hiroshima University School of Medicine, Japan.

Electroconvulsive shock (ECS) therapy is considered to be an effective treatment for depression, but its mechanism of action is still unknown. We investigated the effect of chronic ECS in rats treated for 14 days with dexamethasone (Dex), a glucocorticoid receptor agonist. Chronic injection of sesame oil decreased body weight change and increased serotonin (5-HT)-2A receptor number and DOI (5-HT-2A, 2C receptor agonist)-induced wet-dog shake (WDS) behaviors. Read More

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February 2000