11,876 results match your criteria core promoter

Vitamin D signaling inhibits HBV activity by directly targeting the HBV Core promoter.

J Biol Chem 2021 Sep 22:101233. Epub 2021 Sep 22.

Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India. Electronic address:

Clinical and epidemiological studies support a role for vitamin D in suppressing hepatitis B virus (HBV). This antiviral role of vitamin D is widely attributed to Vitamin D Receptor (VDR)/Retinoid X Receptor (RXR)-mediated regulation of host immunomodulatory genes through Vitamin D Response Elements (VDREs) in their promoters. Here, we investigated the ability of calcitriol (1α,25(OH)D, metabolically activated Vitamin D) to directly regulate HBV activity through this signaling pathway. Read More

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September 2021

Joint changes in RNA, RNA polymerase II, and promoter activity through the cell cycle identify non-coding RNAs involved in proliferation.

Sci Rep 2021 Sep 23;11(1):18952. Epub 2021 Sep 23.

Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology (NTNU), 7491, Trondheim, Norway.

Proper regulation of the cell cycle is necessary for normal growth and development of all organisms. Conversely, altered cell cycle regulation often underlies proliferative diseases such as cancer. Long non-coding RNAs (lncRNAs) are recognized as important regulators of gene expression and are often found dysregulated in diseases, including cancers. Read More

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September 2021

Development of a Cell-Based Biosensor for Residual Detergent Detection in Decellularized Scaffolds.

ACS Synth Biol 2021 Sep 22. Epub 2021 Sep 22.

Department of Medical Biotechnology, School of Paramedicine, Guilan University of Medical Sciences, 4256 Rasht, Iran.

Ex vivo engineering of organs that uses decellularized whole organs as a scaffold with autologous stem cells is a potential alternative to traditional transplantation. However, one of the main challenges in this approach is preparing cytocompatible scaffolds. So far, high-precision and specific evaluation methods have not been developed for this purpose. Read More

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September 2021

TERT Copy Number Alterations, Promoter Mutations and Rearrangements in Adrenocortical Carcinomas.

Endocr Pathol 2021 Sep 22. Epub 2021 Sep 22.

Department of Pathology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY, 10065, USA.

Molecular characterization of adrenocortical carcinomas (ACC) by The Cancer Genome Atlas (TCGA) has highlighted a high prevalence of TERT alterations, which are associated with disease progression. Herein, 78 ACC were profiled using a combination of next generation sequencing (n = 76) and FISH (n = 9) to assess for TERT alterations. This data was combined with TCGA dataset (n = 91). Read More

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September 2021

Androgen receptor transactivates KSHV noncoding RNA PAN to promote lytic replication-mediated oncogenesis: A mechanism of sex disparity in KS.

PLoS Pathog 2021 Sep 20;17(9):e1009947. Epub 2021 Sep 20.

Key Laboratory of Gastrointestinal Cancer (Ministry of Education), School of Basic Medical Sciences, Fujian Medical University, Fuzhou, P.R. China.

Kaposi's sarcoma-associated herpesvirus (KSHV) preferentially infects and causes Kaposi's sarcoma (KS) in male patients. However, the biological mechanisms are largely unknown. This study was novel in confirming the extensive nuclear distribution of the androgen receptor (AR) and its co-localization with viral oncoprotein of latency-associated nuclear antigen in KS lesions, indicating a transcription way of AR in KS pathogenesis. Read More

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September 2021

Intratumor Epigenetic Heterogeneity-A Panel Gene Methylation Study in Thyroid Cancer.

Front Genet 2021 3;12:714071. Epub 2021 Sep 3.

Department of Gastroenterology and Hepatology, Chinese PLA General Hospital, Beijing, China.

Background: Thyroid cancer (TC) is the most common endocrine malignancy, and the incidence is increasing very fast. Surgical resection and radioactive iodine ablation are major therapeutic methods, however, around 10% of differentiated thyroid cancer and all anaplastic thyroid carcinoma (ATC) are failed. Comprehensive understanding the molecular mechanisms may provide new therapeutic strategies for thyroid cancer. Read More

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September 2021

miR-2337 induces TGF-β1 production in granulosa cells by acting as an endogenous small activating RNA.

Cell Death Discov 2021 Sep 18;7(1):253. Epub 2021 Sep 18.

College of Animal Science and Technology, Nanjing Agricultural University, 210095, Nanjing, China.

Transforming growth factor-β1 (TGF-β1) is essential for ovarian function and female fertility in mammals. Herein, we identified three completely linked variants, including two known variants referred to as c.1583A > G and c. Read More

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September 2021

Synthetic essentiality between PTEN and core dependency factor PAX7 dictates rhabdomyosarcoma identity.

Nat Commun 2021 Sep 17;12(1):5520. Epub 2021 Sep 17.

Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.

PTEN promoter hypermethylation is nearly universal and PTEN copy number loss occurs in ~25% of fusion-negative rhabdomyosarcoma (FN-RMS). Here we show Pten deletion in a mouse model of FN-RMS results in less differentiated tumors more closely resembling human embryonal RMS. PTEN loss activated the PI3K pathway but did not increase mTOR activity. Read More

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September 2021

Pharmacological preconditioning by TERT inhibitor BIBR1532 confers neuronal ischemic tolerance through TERT-mediated transcriptional reprogramming.

J Neurochem 2021 Sep 17. Epub 2021 Sep 17.

Clinical Neuroscience Institute, The First Affiliated Hospital of Jinan University, Guangzhou, China.

After a sublethal ischemic preconditioning (IPC) stimulus, the brain has a remarkable capability of acquiring tolerance to subsequent ischemic insult by establishing precautionary self-protective mechanism. Understanding this endogenous mechanism would reveal novel and effective neuroprotective targets for ischemic brain injury. Our previous study has implied that telomerase reverse transcriptase (TERT) is associated with IPC-induced tolerance. Read More

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September 2021

The effect of genetically modified platelet-derived growth factor-BB over-expressing mesenchymal stromal cells during core decompression for steroid-associated osteonecrosis of the femoral head in rabbits.

Stem Cell Res Ther 2021 Sep 15;12(1):503. Epub 2021 Sep 15.

Department of Orthopaedic Surgery, Stanford University School of Medicine, 300 Pasteur Drive, Edwards R155, Stanford, CA, 94305, USA.

Background: Approximately one third of patients undergoing core decompression (CD) for early-stage osteonecrosis of the femoral head (ONFH) experience progression of the disease, and subsequently require total hip arthroplasty (THA). Thus, identifying adjunctive treatments to optimize bone regeneration during CD is an unmet clinical need. Platelet-derived growth factor (PDGF)-BB plays a central role in cell growth and differentiation. Read More

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September 2021

The fate of notch-1 transcript is linked to cell cycle dynamics by activity of a natural antisense transcript.

Nucleic Acids Res 2021 Sep 14. Epub 2021 Sep 14.

IDR/Westmead Institute for Medical Research, NSW 2145, Australia.

A core imprint of metazoan life is that perturbations of cell cycle are offset by compensatory changes in successive cellular generations. This trait enhances robustness of multicellular growth and requires transmission of signaling cues within a cell lineage. Notably, the identity and mode of activity of transgenerational signals remain largely unknown. Read More

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September 2021

Efficiency of graft-transmitted JcFT for floral induction in woody perennial species of the Jatropha genus depends on transport distance.

Tree Physiol 2021 Sep 9. Epub 2021 Sep 9.

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Menglun, Yunnan 666303, China.

Flowering Locus T (FT) promotes flowering by integrating six genetic pathways. In Arabidopsis, the FT protein is transported from leaves to shoot apices and induces flowering. However, contradictory conclusions about floral induction via graft-transmitted FT in trees were reported in previous studies. Read More

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September 2021

MicroRNA-29a in Osteoblasts Represses High-Fat Diet-Mediated Osteoporosis and Body Adiposis through Targeting Leptin.

Int J Mol Sci 2021 Aug 24;22(17). Epub 2021 Aug 24.

Core Laboratory for Phenomics and Diagnostic, Department of Medical Research, Chang Gung University College of Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan.

Skeletal tissue involves systemic adipose tissue metabolism and energy expenditure. MicroRNA signaling controls high-fat diet (HFD)-induced bone and fat homeostasis dysregulation remains uncertain. This study revealed that transgenic overexpression of miR-29a under control of osteocalcin promoter in osteoblasts (miR-29aTg) attenuated HFD-mediated body overweight, hyperglycemia, and hypercholesterolemia. Read More

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Analysis of the proximal promoter of the human colon-specific B4GALNT2 (Sd synthase) gene: B4GALNT2 is transcriptionally regulated by ETS1.

Biochim Biophys Acta Gene Regul Mech 2021 Sep 7;1864(11-12):194747. Epub 2021 Sep 7.

Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F-59000 Lille, France. Electronic address:

Background: The Sd antigen and corresponding biosynthetic enzyme B4GALNT2 are primarily expressed in normal colonic mucosa and are down-regulated to a variable degree in colon cancer tissues. Although their expression profile is well studied, little is known about the underlying regulatory mechanisms.

Methods: To clarify the molecular basis of Sd expression in the human gastrointestinal tract, we investigated the transcriptional regulation of the human B4GALNT2 gene. Read More

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September 2021

Mechanical strain-mediated reduction in RANKL expression is associated with RUNX2 and BRD2.

Gene 2020 Dec 16;763S:100027. Epub 2020 Jan 16.

Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA. Electronic address:

Mechanical loading-related strains trigger bone formation by osteoblasts while suppressing resorption by osteoclasts, uncoupling the processes of formation and resorption. Osteocytes may orchestrate this process in part by secreting sclerostin (SOST), which inhibits osteoblasts, and expressing receptor activator of nuclear factor-κB ligand (RANKL/TNFSF11) which recruits osteoclasts. Both SOST and RANKL are targets of the master osteoblastic transcription factor RUNX2. Read More

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December 2020

Profiling of chromatin accessibility identifies transcription factor binding sites across the genome of Aspergillus species.

BMC Biol 2021 Sep 6;19(1):189. Epub 2021 Sep 6.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou, 510006, China.

Background: The identification of open chromatin regions and transcription factor binding sites (TFBs) is an important step in understanding the regulation of gene expression in diverse species. ATAC-seq is a technique used for such purpose by providing high-resolution measurements of chromatin accessibility revealed through integration of Tn5 transposase. However, the existence of cell walls in filamentous fungi and associated difficulty in purifying nuclei have precluded the routine application of this technique, leading to a lack of experimentally determined and computationally inferred data on the identity of genome-wide cis-regulatory elements (CREs) and TFBs. Read More

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September 2021

SPT5 stabilization of promoter-proximal RNA polymerase II.

Mol Cell 2021 Aug 31. Epub 2021 Aug 31.

Simpson Querrey Institute for Epigenetics, Department of Biochemistry and Molecular Genetics Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. Electronic address:

Based on in vitro studies, it has been demonstrated that the DSIF complex, composed of SPT4 and SPT5, regulates the elongation stage of transcription catalyzed by RNA polymerase II (RNA Pol II). The precise cellular function of SPT5 is not clear, because conventional gene depletion strategies for SPT5 result in loss of cellular viability. Using an acute inducible protein depletion strategy to circumvent this issue, we report that SPT5 loss triggers the ubiquitination and proteasomal degradation of the core RNA Pol II subunit RPB1, a process that we show to be evolutionarily conserved from yeast to human cells. Read More

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The basic helix-loop-helix transcription factor TabHLH1 increases chlorogenic acid and luteolin biosynthesis in Taraxacum antungense Kitag.

Hortic Res 2021 Sep 1;8(1):195. Epub 2021 Sep 1.

Laboratory for Core Technology of TCM Quality Improvement and Transformation, College of Pharmacy, School of Pharmaceutical Sciences, The Third Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China.

Polyphenols are the main active components of the anti-inflammatory compounds in dandelion, and chlorogenic acid (CGA) is one of the primary polyphenols. However, the molecular mechanism underlying the transcriptional regulation of CGA biosynthesis remains unclear. Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyl transferase (HQT2) is the last rate-limiting enzyme in chlorogenic acid biosynthesis in Taraxacum antungense. Read More

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September 2021

NPAS2 ameliorates myocardial ischaemia/reperfusion injury in rats via CX3CL1 pathways and regulating autophagy.

Aging (Albany NY) 2021 08 30;13(16):20569-20584. Epub 2021 Aug 30.

Department of Cardiology, Shanghai East Hospital, Shanghai Tongji University School of Medicine, Shanghai, China.

Background: Myocardial ischemia/reperfusion (I/R) injury is common during the treatment of cardiovascular diseases. Neuronal PAS Domain Protein 2 (NPAS2) is one of the core genes that control the rhythm of the biological clock. NPAS2 also regulates the biological rhythm. Read More

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ATG5 and ATG7 Expression Levels Are Reduced in Cutaneous Melanoma and Regulated by NRF1.

Front Oncol 2021 12;11:721624. Epub 2021 Aug 12.

Institute of Pharmacology, University of Bern, Bern, Switzerland.

Autophagy is a highly conserved cellular process in which intracellular proteins and organelles are sequestered and degraded after the fusion of double-membrane vesicles known as autophagosomes with lysosomes. The process of autophagy is dependent on autophagy-related (ATG) proteins. The role of autophagy in cancer is very complex and still elusive. Read More

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Functional characterization of late embryogenesis abundant genes and promoters in pearl millet (Pennisetum glaucum L.) for abiotic stress tolerance.

Physiol Plant 2021 Aug 29. Epub 2021 Aug 29.

International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, Andhra Pradesh, India.

Late embryogenesis abundant (LEA) genes display distinct functions in response to abiotic stresses in plants. In pearl millet (Pennisetum glaucum L.), a total of 21 PgLEA genes were identified and classified into six groups including LEA1, LEA2, LEA3, LEA5, LEA7, and dehydrins (DHN). Read More

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A novel mechanism for C1GALT1 in the regulation of gastric cancer progression.

Cell Biosci 2021 Aug 26;11(1):166. Epub 2021 Aug 26.

Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, China.

Background: Gastric cancer (GC) is a highly aggressive and lethal disease around the world. High expression of core 1 β 1, 3-galactosyltransferase 1 (C1GALT1), the primary enzyme responsible for protein O-glycosylation, plays a critical role in gastric carcinogenesis. However, proteins that can be O-glycosylated by C1GALT1 in GC have not been completely elucidated. Read More

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Isolation and Characterization of Orthologs and Promoters from the Distylous .

Plants (Basel) 2021 Aug 10;10(8). Epub 2021 Aug 10.

College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China.

Common buckwheat () produces distylous flowers with undifferentiated petaloid tepals, which makes it obviously different from flowers of model species. In model species , () is expressed in petal and stamen and specifies petal and stamen identities during flower development. Combining with our previous studies, we found that small-scale gene duplication (GD) event and alternative splicing (AS) of common buckwheat orthologs resulted in , and . Read More

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L- Gene Expression in Canine Fetal Fibroblasts Promotes Self-Renewal Capacity but Not Tumor Formation.

Cells 2021 Aug 4;10(8). Epub 2021 Aug 4.

College of Medicine and Medical Research Institute, Chungbuk National University, Chungbuk, Cheongju 28644, Korea.

Canines are useful in mammalian preclinical studies because they are larger than rodents and share many diseases with humans. Canine fetal fibroblast cells (CFFs) are an easily accessible source of somatic cells. However, they are easily driven to senescence and become unusable with continuous in vitro culture. Read More

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MicroRNA-29a Mitigates Osteoblast Senescence and Counteracts Bone Loss through Oxidation Resistance-1 Control of FoxO3 Methylation.

Antioxidants (Basel) 2021 Aug 4;10(8). Epub 2021 Aug 4.

Core Laboratory for Phenomics and Diagnostic, Department of Medical Research, College of Medicine, Chang Gung University, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan.

Senescent osteoblast overburden accelerates bone mass loss. Little is understood about microRNA control of oxidative stress and osteoblast senescence in osteoporosis. We revealed an association between microRNA-29a () loss, oxidative stress marker 8-hydroxydeoxyguanosine (8-OHdG), DNA hypermethylation marker 5-methylcystosine (5mC), and osteoblast senescence in human osteoporosis. Read More

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Antithetic hTERT Regulation by Androgens in Prostate Cancer Cells: hTERT Inhibition Is Mediated by the ING1 and ING2 Tumor Suppressors.

Cancers (Basel) 2021 Aug 10;13(16). Epub 2021 Aug 10.

Institute of Human Genetics, Jena University Hospital, 07740 Jena, Germany.

The human telomerase is a key factor during tumorigenesis in prostate cancer (PCa). The androgen receptor (AR) is a key drug target controlling PCa growth and regulates expression, but is described to either inhibit or to activate. Here, we reveal that androgens repress and activate expression in a concentration-dependent manner. Read More

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Pharmacological Inhibition of Core Regulatory Circuitry Liquid-liquid Phase Separation Suppresses Metastasis and Chemoresistance in Osteosarcoma.

Adv Sci (Weinh) 2021 Aug 25:e2101895. Epub 2021 Aug 25.

Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China.

Liquid-liquid phase-separated (LLPS) transcriptional factor assemblies at super-enhancers (SEs) provide a conceptual framework for underlying transcriptional control in mammal cells. However, the mechanistic understanding of LLPS in aberrant transcription driven by dysregulation of SEs in human malignancies is still elusive. By integrating SE profiling and core regulatory circuitry (CRC) calling algorithm, the CRC of metastatic and chemo-resistant osteosarcoma is delineated. Read More

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The Polycomb protein RING1B enables estrogen-mediated gene expression by promoting enhancer-promoter interaction and R-loop formation.

Nucleic Acids Res 2021 Sep;49(17):9768-9782

Sylvester Comprehensive Cancer Center, Biomedical Research Building, 1501 NW 10th Avenue, Miami, FL 33136, USA.

Polycomb complexes have traditionally been prescribed roles as transcriptional repressors, though increasing evidence demonstrate they can also activate gene expression. However, the mechanisms underlying positive gene regulation mediated by Polycomb proteins are poorly understood. Here, we show that RING1B, a core component of Polycomb Repressive Complex 1, regulates enhancer-promoter interaction of the bona fide estrogen-activated GREB1 gene. Read More

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September 2021

Role of the Discriminator Sequence in the Supercoiling Sensitivity of Bacterial Promoters.

mSystems 2021 Aug 24;6(4):e0097821. Epub 2021 Aug 24.

Université de Lyon, INSA-Lyon, Université Claude Bernard Lyon 1, CNRS, UMR5240, MAP, Lyon, France.

DNA supercoiling acts as a global transcriptional regulator that contributes to the rapid transcriptional response of bacteria to many environmental changes. Although a large fraction of promoters from phylogenetically distant species respond to superhelical variations, the sequence or structural determinants of this behavior remain elusive. Here, we focus on the sequence of the "discriminator" element that was shown to modulate this response in several promoters. Read More

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APS (Age, Platelets, 2D Shear-Wave Elastography) Score Predicts Hepatocellular Carcinoma in Chronic Hepatitis B.

Radiology 2021 Aug 24:204700. Epub 2021 Aug 24.

From the Departments of Medical Ultrasonics (T.Z., J.Z., J.J., Z.H., M.W., R.Z.) and Infectious Disease (G.Z.), Third Affiliated Hospital of Sun Yat-Sen University, 600 TianHe Rd, Guangzhou 510630, China; and Department of Environmental Health Sciences, University at Albany, State University of New York, Rensselaer, NY (X.D.).

Background Two-dimensional (2D) shear-wave elastography (SWE) has been considered to be useful in predicting hepatocellular carcinoma (HCC) development in patients with chronic hepatitis B (CHB). Purpose To develop a risk model using 2D SWE to predict HCC in patients with CHB and to compare its predictive value with that of other models. Materials and Methods Patients with biopsy-proven CHB who underwent US and 2D SWE between April 2011 and December 2015 were enrolled in this study. Read More

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