151,638 results match your criteria cord blood

Evaluation and impact of fetal physiology training on fetal heart rate analysis.

J Gynecol Obstet Hum Reprod 2021 Jun 12:102185. Epub 2021 Jun 12.

Univ. Lille, EA 4489 - Perinatal Environment and Health, F-59000 Lille, France; CHU Lille, Department of Obstetrics, F-59000 Lille, France.

Introduction: Evaluation of fetal well-being during labor is based on fetal heart rate (FHR) analysis, which requires physiology expertise. The aim of the present study was to assess medical residents' fetal physiology training in terms of theoretical knowledge, FHR interpretation, and use of second-line examinations.

Methods: This single-center, prospective study of obstetrics and gynecology residents (N=34) at CHU de Lille Hospital (Lille, France) was conducted from November 2017 to November 2018. Read More

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Maternal gestational mercury exposure in relation to cord blood T cell alterations and placental gene expression signatures.

Environ Res 2021 Jun 12:111385. Epub 2021 Jun 12.

Department of Epidemiology, Geisel School of Medicine and the Children's Environmental Health and Disease Prevention Research Center at Dartmouth; Hanover, NH, USA.

The immunotoxic impacts of mercury during early life is poorly understood. We investigated the associations between gestational mercury exposure and frequency of cord blood T cells as well as placental gene expression. Frequency of natural Treg cells was positively associated with prenatal and postpartum mercury toenail concentrations. Read More

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Elevated Anti-Müllerian Hormone Levels in Newborns of Women with Polycystic Ovary Syndrome: a Systematic Review and Meta-analysis Based on Observational Studies.

Reprod Sci 2021 Jun 15. Epub 2021 Jun 15.

Department of Obstetrics and Gynaecology, West China Second University Hospital, Sichuan University, Chengdu, China.

We performed this updated systematic review and meta-analysis to evaluate anti-Müllerian hormone levels (AMH) in newborns of mothers with polycystic ovary syndrome (PCOS) compared with healthy controls. A search of the literature was conducted in the PubMed, MEDLINE, EMBASE, Cochrane Library, CBM, CNKI, WANFANG, and VIP for articles to assess AMH levels in offspring of PCOS and non-PCOS mothers irrespective of language. These databases were searched from their inception to December 7, 2020. Read More

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A case report of prenatal diagnosis of fetal alloimmune thrombocytopenia: A CARE-compliant article.

Medicine (Baltimore) 2021 Jun;100(24):e26092

Department of Obstetrics, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Rationale: Fetal alloimmune thrombocytopenia (FAIT) is a serious life-threatening disease caused by platelet-antigen incompatibility between the mother and fetus. FAIT can lead to fetal thrombocytopenia, intracranial hemorrhage (ICH), fetal death and severe neurological disorders after birth. Noninvasive prenatal diagnosis technology has not been widely used in China, and thus few cases of FAIT can be diagnosed prenatally. Read More

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Combined impact of HLA-allele matching and the CD34-positive cell dose on optimal unit selection for single-unit cord blood transplantation in adults.

Leuk Lymphoma 2021 Jun 15:1-10. Epub 2021 Jun 15.

Central Japan Cord Blood Bank, Seto, Japan.

The combined effects of HLA-allele matching at six-loci (HLA-A, -B, -C, -DRB1, -DQB1, and -DPB1) and CD34 cell dose on clinical outcomes were analyzed in 1,226 adult cases with single-unit unrelated cord blood transplantation. In the six-loci analysis, low HLA-allele matches did not significantly increase the overall mortality compared to higher matches, whereas in the five-loci analysis excluding HLA-DPB1, they caused a higher overall mortality (HR 1.42,  = . Read More

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Blood pressure and cardiac output during caesarean delivery under spinal anaesthesia: a prospective cohort study.

BMJ Open 2021 06 14;11(6):e046102. Epub 2021 Jun 14.

Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.

Objectives: We have previously established a method to measure transfer of nutrients between mother, placenta and fetus in vivo. The method includes measurements of maternal and fetal blood flow by Doppler ultrasound prior to spinal anaesthesia. Spinal anaesthesia affects maternal blood pressure and cardiac output. Read More

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Is it necessary to add the eluate testing to the direct antiglobulin test to improve the detection of maternal erythrocyte alloantibodies?

Transfus Apher Sci 2021 Jun 3:103177. Epub 2021 Jun 3.

University of Antioquia, Medellín, Colombia.

Background: The screening of umbilical cord blood samples by the Direct Antiglobulin Test (DAT) is the reference tool for the identification of maternal erythrocyte alloantibodies present in erythrocytes; however, its diagnostic usefulness is controversial.

Objective: To evaluate the diagnostic validity, safety, and efficiency of the eluate testing (detection of antibody in erythrocyte eluates by the Indirect Antiglobulin Test/IAT) in cord blood samples for detection of maternal erythrocyte alloantibodies in comparison with the DAT.

Materials And Methods: Evaluation study of diagnostic tests. Read More

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Could Improved Microcirculation Reverse the Effects of Fetal Alcohol Syndrome? A Review.

CNS Spectr 2021 Apr;26(2):173

University of Colorado at Boulder, Boulder, CO, USA.

Fetal alcohol spectrum disorders, (FASDs) are a spectrum of neurodevelopmental and cognitive conditions, caused by the consumption of alcohol in varying amounts in utero that affect an estimated 1 - 5% of the general population in the United States. The effects of the spectrum of disorders are lifelong, resulting in often significant learning difficulties, cognitive deficits, and behavioral issues. It is known that blood flow and microcirculation to the brain are affected by the consumption of alcohol in utero, and that microcirculation and neurology are intimately linked, with changes in the former having drastic effects on the latter. Read More

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Discordant structural chromosomal aberrations in chorionic villi and amniotic fluid leading to a formation of an isochromosome 21: a case report.

Mol Cytogenet 2021 Jun 14;14(1):30. Epub 2021 Jun 14.

Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.

Background: Fetoplacental discrepancies occur in approximately 1-2% of analyzed prenatal cases. They are typically due to confined placental mosaicism, where an aberration is observed in the placental cells but not found in the fetal cells. Confined placental mosaicism usually involves aneuploidies and more sparsely structural chromosomal aberrations. Read More

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Ambient PM gross β-activity and glucose levels during pregnancy.

Environ Health 2021 Jun 14;20(1):70. Epub 2021 Jun 14.

Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, 02115, USA.

Background: Exposure to ionizing radiation has been associated with insulin resistance and type 2 diabetes. In light of recent work showing an association between ambient particulate matter (PM) gross β-activity and gestational diabetes mellitus (GDM) among pregnant women, we examined pregnancy glucose levels in relation to PM gross β-activity to better understand this pathway.

Methods: Our study included 103 participants receiving prenatal care at Beth Israel Deaconess Medical Center in Boston, MA. Read More

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Simultaneous umbilical blood flow during normal pregnancy in sheep and goat foetuses using non-invasive colour Doppler ultrasound.

Anim Reprod 2018 Aug 16;15(2):148-155. Epub 2018 Aug 16.

Institute of Reproductive Biology, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.

The characteristics of umbilical blood flow (UM) was investigated using 18 (25 foetuses) pregnant ewes and 20 (41 foetus) pregnant goats transrectal non-invasive color Doppler ultrasonographic examinations were done frequently between 2 and 8 week after breeding and then transabdominally until parturition. Colour Doppler velocimetery includes blood flow volume (BFV), time averaged maximum velocity (TAMV), resistance index (RI), pulsatility index (PI), time average of mean (TAMEAN) and impedance of blood flow (PS/ED or AB ratio). Also a qualitative evaluation of UM blood flow indicating increases (P < 0. Read More

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Effects of fetal number on acute phase proteins, cortisol, and hematological parameters in ewes during the periparturient period.

Anim Reprod Sci 2021 Jun 8;231:106793. Epub 2021 Jun 8.

Institute of Agricultural Biology and Biotechnology (IBBA), National Research Council (CNR), 56124, Pisa, Italy.

Many subclinical diseases associated with inflammation occur in sheep during the periparturient period. Clinical symptoms are usually detected at an advanced stage of the disease; therefore, there are considerable risks of permanent health disorders in fetuses or dams. Determination of acute phase proteins (APPs) as markers of inflammation may allow for an earlier diagnosis and effective treatment. Read More

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A case of Philadelphia chromosome-positive acute lymphocytic leukaemia with type I CD36 deficiency.

Vox Sang 2021 Jun 14. Epub 2021 Jun 14.

Department of Transfusion Medicine, Tokyo Metropolitan Tama Medical Center, Tokyo, Japan.

Background: CD36 is a glycoprotein expressed on platelets and monocytes of the blood. There are two types of CD36 deficiency, type I and type II. Individuals with type I-deficiency do not express CD36 in any cell type and can produce the CD36 antibody, which causes pathological conditions, such as fetal/neonatal alloimmune thrombocytopenia (FNAIT) and platelet transfusion refractory (PTR), through antigenic exposure via transfusion or pregnancy. Read More

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Colony-forming units optimization and human papillomavirus detection in umbilical cord blood.

Gac Med Mex 2021 ;157(1):29-34

Center of Infectious Diseases Research, Secretaría de Salud, Cuernavaca, Morelos, Mexico.

Introduction: Analysis of several parameters is required for adequate quality control in umbilical cord blood units (UCBU) when used for therapeutic purposes.

Objective: To optimize colony-forming units (CFU) from clonogenic cultures and to detect the human papillomavirus (HPV) genome in UCBU.

Methods: One hundred and forty-one umbilical cord blood (UCB), segment or CFU samples from UCBU clonogenic cultures were included. Read More

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January 2021

Genetic reprogramming of cord blood derived endothelial colony forming cells towards human induced pluripotent stem cells using episomal plasmids.

J Pak Med Assoc 2021 Apr;71(4):1081-1088

Department of Pathology, Dow International Medical College, Dow University of Health Sciences (Ojha Campus), Karachi, Pakistan.

Objective: This study aimed to isolate human umbilical cord blood derived endothelial colony forming cells (ECFCs) followed by their integration free reprogramming towards induced pluripotent stem cells (iPSCs) and molecular characterization of both cell types using multicolour flowcytometery and immunofluorescence respectively.

Methods: The cord blood was collected from 37-39 weeks of gestational ages after C-section ex-utero from Dow University Hospital. The ECFCs isolated after ficoll based separation of cord blood mononuclear cells (CBMNCs) which on emergence characterized through flow cytometry and reprogrammed towards induced pluripotent stem cells (iPSCs) using episomal vectors, the iPSCs were characterized using immunofluorescence. Read More

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Pyruvate kinase deficiency in children.

Pediatr Blood Cancer 2021 Jun 14:e29148. Epub 2021 Jun 14.

Dana-Farber/Boston Children's Cancer and Blood Disorder Center, Boston, Massachusetts, USA.

Background: Pyruvate kinase deficiency (PKD) is a rare, autosomal recessive red blood cell enzyme disorder, which leads to lifelong hemolytic anemia and associated complications from the disease and its management.

Methods: An international, multicenter registry enrolled 124 individuals younger than 18 years old with molecularly confirmed PKD from 29 centers. Retrospective and prospective clinical data were collected. Read More

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Retrospective evaluation of feline dystocia: clinicopathologic findings and neonatal outcomes in 35 cases (2009-2020).

J Feline Med Surg 2021 Jun 14:1098612X211024154. Epub 2021 Jun 14.

Michigan State University College of Veterinary Medicine, East Lansing, MI, USA.

Objectives: The aim of this study was to describe the characteristics of cases of feline dystocia presenting to a university emergency service.

Methods: The medical records of queens presenting for dystocia between January 2009 and September 2020 were reviewed. Data collected included queen signalment, presenting complaints, treatments, and maternal and neonatal outcomes. Read More

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A Chemically Defined, Xeno- and Blood-Free Culture Medium Sustains Increased Production of Small Extracellular Vesicles From Mesenchymal Stem Cells.

Front Bioeng Biotechnol 2021 26;9:619930. Epub 2021 May 26.

Cells for Cells, Santiago, Chile.

Cell therapy is witnessing a notable shift toward cell-free treatments based on paracrine factors, in particular, towards small extracellular vesicles (sEV), that mimic the functional effect of the parental cells. While numerous sEV-based applications are currently in advanced preclinical stages, their promised translation depends on overcoming the manufacturing hurdles posed by the large-scale production of purified sEV. Unquestionably, the culture medium used with the parental cells plays a key role in the sEV's secretion rate and content. Read More

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A substitution mutation in LRP8 gene is significantly associated with susceptibility to familial myocardial infarction.

ARYA Atheroscler 2020 Nov;16(6):301-305

Assistant Professor, Department of Genetics, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.

Background: Myocardial infarction (MI) is a multifactorial disease caused by the suspension of blood circulation in a part of the myocardium. Understanding the genetic basis of MI can provide insight regarding the pathogenesis of the disease. The aim of this study was to investigate the association between pathogenic mutations and early-onset MI in five families with familial MI and without common MI risk factor. Read More

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November 2020

Prenatal Genetic Counseling in a Chinese Pregnant Woman With Rare Thalassemia: A Case Report.

Front Genet 2021 28;12:670168. Epub 2021 May 28.

Department of Endocrinology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Prenatal genetic counseling can be difficult, especially when it is related to fetuses with a rare thalassemia. An intronic variant located far from obvious regulatory sequences in the gene could be very difficult to evaluate as it may affect the mRNA processing or cause β-thalassemia (β-thal). In the present study, a Chinese pregnant woman with HbJ-Bangkok and a very rare change in the second intron of the gene [IVS-II-806(G>C), NM_000518. Read More

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Co-Inheritance of α-thalassemia gene mutation in patients with sickle cell Disease: Impact on clinical and hematological variables.

Niger J Clin Pract 2021 Jun;24(6):874-882

Department of Pediatrics, College of Medicine, University of Basra, Basra, Iraq.

Background: Sickle cell disease (SCD) is a monogenic, phenotypically highly variable disease with multisystem pathology. The phenotypic heterogeneity of SCD is attributed to environmental and genetic factors such as fetal hemoglobin and co-inheritance of α-thalassemia.

Objectives: To look for different types of α-thalassemia gene mutations among SCD patients and evaluate the influence of the co-inheritance of α-thalassemia on clinical and hematological variables. Read More

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Race as a factor in donor selection and survival of children with hematologic malignancies undergoing hematopoietic stem cell transplant in Florida.

Pediatr Blood Cancer 2021 Jun 14:e29180. Epub 2021 Jun 14.

Center for Cell and Gene Therapy for Nonmalignant Conditions, Blood and Marrow Transplant Program, Cancer & Blood Disorders Institute, Johns Hopkins All Children's Hospital, St. Petersburg, Florida, USA.

Background: Previous studies have explored posthematopoietic cell transplant (HCT) outcomes by race in adults; however, pediatric data addressing this topic are scarce.

Procedure: This retrospective registry study included 238 White (W) and 57 Black (B) children with hematologic malignancies (HM) receiving first allogeneic HCT between 2010 and 2019 at one of the five Florida pediatric HCT centers.

Results: We found no differences between W and B children in transplant characteristics, other than donor type. Read More

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Global proteomic analyses of human cytotrophoblast differentiation/invasion.

Development 2021 Jun 14. Epub 2021 Jun 14.

Center for Reproductive Sciences, University of California, San Francisco, California 94143, USA.

During human pregnancy, cytotrophoblasts (CTBs) from the placenta differentiate into specialized subpopulations that play critical roles in proper fetal growth and development. A subset of these CTBs differentiate along an invasive pathway, penetrating the decidua and anchoring the placenta to the uterus. A critical hurdle in pregnancy is the ability of these cells to migrate, invade, and remodel spiral arteries, ensuring adequate blood flow to nourish the developing fetus. Read More

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Early Predictors of Gestational Diabetes Mellitus in IVF-Conceived Pregnancies.

Endocr Pract 2021 Jun 17;27(6):579-585. Epub 2020 Dec 17.

Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Clinical Sciences Research Laboratories, University Hospitals Coventry and Warwickshire, Clifford Bridge Road, Coventry, CV2 2DX, United Kingdom.

Objective: Gestational diabetes mellitus (GDM) is associated with adverse maternal and fetal outcomes. This study aimed to identify early and reliable GDM predictors that would enable implementation of preventive and management measures.

Methods: The participants were a 28-week prospective cohort of in vitro fertilization (IVF)-conceived pregnant women (≤39 years, body mass index [BMI] 18. Read More

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Gestational acrylamide exposure and biomarkers of fetal growth: Probing the mechanism underlying the association between acrylamide and reduced fetal growth.

Environ Int 2021 Jun 11;155:106668. Epub 2021 Jun 11.

Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium; Department of Public Health & Primary Care, Leuven University, Leuven, Belgium.

Introduction: Four epidemiological studies have shown a negative association between prenatal acrylamide exposure and birth size. In order to shed light on the possible underlying mechanism(s), we analysed associations between acrylamide biomarkers and biomarkers related to fetal growth.

Methods: In newborns of the ENVIRONAGE birth cohort (n ranges from 215 to 434), we investigated the association between prenatal acrylamide exposure (acrylamide and glycidamide hemoglobin adduct levels in cord blood) and thyroid hormones (TSH, T3, T4 and the ratio of T4 to T3 in cord plasma), insulin-related factors (cord plasma insulin and IGF1, and placental IGF2), neurotrophins (cord plasma BDNF, and placental NGF, NT3 and NT4), and cord plasma homocysteine and progesterone, using multiple linear regression analysis. Read More

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Characteristics of cases with and without calcification in spinal meningiomas.

J Clin Neurosci 2021 Jul 29;89:20-25. Epub 2021 Apr 29.

Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, 65, Tsurumai-cho, Showa-ku, Nagoya 466-8560, Japan.

Purpose: To quantify calcification in spinal meningiomas using Hounsfield unit (HU) values on CT, and to analyze the characteristics of cases with and without calcification and with different histologic subtypes.

Methods: The subjects were 53 patients who underwent surgical resection of spinal meningioma between January 1999 and December 2019. Clinical and surgical data were collected, and all patients were examined neurologically preoperatively and at final follow-up using the modified McCormick scale and the American Spinal Injury Association scale. Read More

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Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia.

BMC Infect Dis 2021 Jun 12;21(1):564. Epub 2021 Jun 12.

School of Medical Laboratory Sciences, Jimma University, Jimma, Ethiopia.

Background: Listeria monocytogenes (LM) has come to be a major public health issue of at-risk groups, causing high morbidity and mortality. Despite this data, studies are very limited in developing countries like Ethiopia. Thus, we aimed to isolate and characterize LM in terms of antibiogram and biofilm formation among pregnant women with fever, women with a history of spontaneous abortion, women with a history of fetal loss, and women with preterm delivery at Jimma University Medical Center (JUMC), southwest Ethiopia. Read More

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Anti-beta 2 glycoprotein 1 domain IgA positive antiphospholipid syndrome secondary to incomplete Behcet's disease: a case report.

Ann Palliat Med 2021 Jun 8. Epub 2021 Jun 8.

Department of Rheumatology, the First Hospital of Hebei Medical University, Shijiazhuang, China.

Antiphospholipid syndrome (APS) is an autoimmune systemic disorder characterized by arterial, venous, or small vessel thrombosis, and/or recurrent early pregnancy loss, fetal loss, or pregnancy morbidity. APS is induced by persistent positive antiphospholipid antibodies (aPL), the main being lupus anticoagulant (LA), and/or anticardiolipin (aCL) antibodies, and/or anti-beta 2 glycoprotein 1 (β2-GP1) antibodies. Some studies have shown that the incidence of APS is about 5 new cases per 100,000 persons per year, and the prevalence is around 40-50 cases per 100,000 persons. Read More

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Precision dosing of methadone during pregnancy: A pharmacokinetics virtual clinical trials study.

J Subst Abuse Treat 2021 Jun 3;130:108521. Epub 2021 Jun 3.

Humankind Charity, Inspiration House, Unit 22, Bowburn North Industrial Estate, Durham DH6 5PF, United Kingdom.

Background: Methadone use for the management of opioid dependency during pregnancy is commonplace. Methadone levels are altered during pregnancy due to changes in maternal physiology. Despite this, a paucity of data exist regarding the most appropriate optimal dosing regimens during pregnancy. Read More

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