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Single-shot quantitative phase imaging as an extension of differential interference contrast microscopy.

Genes Cells 2021 Jun 4. Epub 2021 Jun 4.

Central Research Laboratory, Hamamatsu Photonics K.K, 5000 Hirakuchi, Hamakita-ku, Hamamatsu, Shizuoka, 434-8601, Japan.

Various studies have been conducted to obtain quantitative phase information based on differential interference contrast (DIC) microscopy. As one such attempt, we propose in this study a single-shot quantitative phase imaging (QPI) method by combining two developments. First, an add-on optical system to a commercialized DIC microscope was developed to perform quantitative phase gradient imaging (QPGI) with single image acquisition using a polarization camera. Read More

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Characithecium spp. (Monogenea: Dactylogyridae) from Astyanax bimaculatus (Characiformes: Characidae) in Northeast Brazil, with Description of a New Species.

Acta Parasitol 2021 May 12. Epub 2021 May 12.

Department of Biological Sciences, Regional University of Cariri (Urca), Rua Coronel Antônio Luiz, n° 1,161, Crato, Ceará, 63105-000, Brazil.

Purpose: To date, seven species of Characithecium Mendonza-Franco, Reina and Torchin, 2009 (Monogenea, Dactylogyridae) are valid, parasitizing mainly fishes of the family Characidae (Characiformes) in the Neotropical region. During a parasitological study of fishes in the Batateiras River and Carás stream, Ceará state, Brazil, two species of Characithecium were collected from the gills of Astyanax bimaculatus Linnaeus, 1758 (Characidae). The purpose of the present study is to describe a new species of monogenean of the genus Characithecium and report Characithecium costaricensis Mendoza-Franco, Reina and Torchin, 2009 in A. Read More

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[Disseminated Bone Marrow Carcinomatosis from Colon Cancer-A Case Report].

Gan To Kagaku Ryoho 2021 Apr;48(4):602-604

Dept. of Surgery, Saiseikai Senri Hospital.

A 77‒year‒old man came to our hospital with complaints of abdominal pain and difficulty to defecate. Abdominal CT scan showed an abnormal region in the ascending colon, which was suspected to be an ascending colon cancer. D‒dimer was remarkably high, and the platelet count was 63,000/μL; these results suggested disseminated intravascular coagulation caused by tumor activation. Read More

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Case Report: ANXA2 Associated Life-Threatening Coagulopathy With Hyperfibrinolysis in a Patient With Non-APL Acute Myeloid Leukemia.

Front Oncol 2021 15;11:666014. Epub 2021 Apr 15.

Department of Internal Medicine I, University Hospital Carl Gustav Carus, TU Dresden, Dresden, Germany.

Patients with acute promyelocytic leukemia (APL) often present with potentially life-threatening hemorrhagic diathesis. The underlying pathomechanisms of APL-associated coagulopathy are complex. However, two pathways considered to be APL-specific had been identified: 1) annexin A2 (ANXA2)-associated hyperfibrinolysis and 2) podoplanin (PDPN)-mediated platelet activation and aggregation. Read More

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ETosis in tambaqui Colossoma macropomum: A programmed cell death pathway and approach of leukocytes immune response.

Microb Pathog 2021 Jun 27;155:104918. Epub 2021 Apr 27.

São Paulo State University (UNESP), Aquaculture Center of UNESP, Laboratory of Microbiology and Parasitology of Aquatic Organisms, São Paulo, 14884-900, Brazil. Electronic address:

Tambaqui Colossoma macropomum is the most cultivated native fish in South America and Aeromonas hydrophila is one of the main bacteria infecting tropical fish. Despite the economic importance of this round fish, to date, there has been a paucity of investigations into haematological changes in tambaqui. In this study, detailed blood analyses (0 h, 6 h, 24 h, 7 d and 14 d) following intraperitoneal challenge with A. Read More

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A Rare Case of Cerebral Venous Thrombosis and Disseminated Intravascular Coagulation Temporally Associated to the COVID-19 Vaccine Administration.

J Pers Med 2021 Apr 8;11(4). Epub 2021 Apr 8.

Department of Medicine of Precision, School of Medicine, "Luigi Vanvitelli" University of Campania, 80147 Naples, Italy.

Globally, at the time of writing (20 March 2021), 121.759.109 confirmed COVID-19 cases have been reported to the WHO, including 2. Read More

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Gut-specific arylphorin mediates midgut regenerative response against Cry-induced damage in Achaea janata.

Comp Biochem Physiol B Biochem Mol Biol 2021 Aug-Sep;255:110600. Epub 2021 Apr 10.

Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.

Development of insect resistance to biopesticides is a current and pertinent global issue. Earlier, it was established that lepidopteran larvae can recover from Bt intoxication via a midgut regenerative response and subsequently generate resistance. Molecular aspects of restoration of the midgut integrity following toxin exposure are emerging recently. Read More

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Novel life cycle stages of Colpodella sp. (Apicomplexa) identified using Sam-Yellowe's trichrome stains and confocal and electron microscopy.

Int Microbiol 2021 Apr 9. Epub 2021 Apr 9.

Cleveland Clinic Lerner Research Institute, Cleveland, OH, 44195, USA.

Colpodella spp. are free-living flagellates closely related to the apicomplexans. Human infections by Colpodella sp. Read More

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[Analysis of the major clinical-pathological discrepancies in autopsies of adults in a tertiary referral hospital between 2008-2017].

Rev Esp Patol 2021 Apr-Jun;54(2):92-101. Epub 2020 Mar 31.

Facultad de Medicina de la Universidad de Oviedo, Oviedo, España; Servicio de Anatomía Patológica, Hospital Universitario Central de Asturias, Oviedo, España. Electronic address:

Objective: To compare and contrast clinical diagnoses with autopsy findings in order to identify unexpected, relevant discrepancies.

Material And Method: A retrospective observational study of the revision of autopsies of adults and their respective medical records in order to classify them according to referral department and Goldman's classification was carried out at the Central University Hospital of Asturias between 2008-2017.

Results: 694 (52. Read More

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Atg9-centered multi-omics integration reveals new autophagy regulators in .

Autophagy 2021 Mar 15:1-24. Epub 2021 Mar 15.

Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei China.

In , Atg9 is an important autophagy-related (Atg) protein, and interacts with hundreds of other proteins. How many Atg9-interacting proteins are involved in macroautophagy/autophagy is unclear. Here, we conducted a multi-omic profiling of Atg9-dependent molecular landscapes during nitrogen starvation-induced autophagy, and identified 290 and 256 genes to be markedly regulated by in transcriptional and translational levels, respectively. Read More

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No effect in primary stability after increasing interference fit in cementless TKA tibial components.

J Mech Behav Biomed Mater 2021 06 4;118:104435. Epub 2021 Mar 4.

Radboud University Medical Center, Radboud Institute for Health Sciences, Orthopaedic Research Lab, Nijmegen, the Netherlands.

Cementless total knee arthroplasty (TKA) implants rely on interference fit to achieve initial stability. However, the optimal interference fit is unknown. This study investigates the effect of using different interference fit on the initial stability of tibial TKA implants. Read More

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Single gold nanostars with multiple branches as multispectral orientation probes in single-particle rotational tracking.

Chem Commun (Camb) 2021 Apr 2;57(26):3263-3266. Epub 2021 Mar 2.

Advanced Nano-Bio-Imaging and Spectroscopy Laboratory, Department of Chemistry, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan 44610, Republic of Korea.

Herein, we performed a single-particle correlation study to characterize the optical properties of gold nanostars (AuNSs) with multiple sharp branches under dark-field (DF) and differential interference contrast (DIC) microscopy, and to examine their use as multispectral orientation probes. We presented the polarization-dependent, periodic DIC images and intensities of single AuNSs at their localized surface plasmon resonance (LSPR) wavelengths with high sensitivity. Furthermore, we demonstrated that single AuNSs protrude multiple branches that can be used as individual sensors with DIC polarization anisotropy. Read More

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Statistical image processing quantifies the changes in cytoplasmic texture associated with aging in Caenorhabditis elegans oocytes.

BMC Bioinformatics 2021 Feb 17;22(1):73. Epub 2021 Feb 17.

Department of Computational Science, Graduate School of System Informatics, Kobe University, Kobe, Hyogo, 657-8501, Japan.

Background: Oocyte quality decreases with aging, thereby increasing errors in fertilization, chromosome segregation, and embryonic cleavage. Oocyte appearance also changes with aging, suggesting a functional relationship between oocyte quality and appearance. However, no methods are available to objectively quantify age-associated changes in oocyte appearance. Read More

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February 2021

Intraductal papillary neoplasm of intrahepatic bile ducts complicated by chronic disseminated intravascular coagulation and thrombosis: A case report.

Medicine (Baltimore) 2021 Feb;100(5):e24454

Center of Hepatopancreatobiliary Diseases.

Rationale: Intraductal papillary neoplasm of the bile ducts (IPNB) is a relatively rare tumor that is clinically characterized by digestive symptoms. The concurrent occurrence of chronic disseminated intravascular coagulation (DIC) with thrombosis is an extremely rare combination, reported in patients with IPNB. The clinical features of chronic DIC include microangiopathic hemolytic anemia, thrombocytopenia, and hypofibrinogenemia. Read More

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February 2021

Complement Activation in Kidneys of Patients With COVID-19.

Front Immunol 2020;11:594849. Epub 2021 Jan 29.

Department of Nephropathology, Institute of Pathology, Friedrich-Alexander-University (FAU) Erlangen-Nürnberg, Erlangen, Germany.

Most patients who became critically ill following infection with COVID-19 develop severe acute respiratory syndrome (SARS) attributed to a maladaptive or inadequate immune response. The complement system is an important component of the innate immune system that is involved in the opsonization of viruses but also in triggering further immune cell responses. Complement activation was seen in plasma adsorber material that clogged during the treatment of critically ill patients with COVID-19. Read More

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February 2021

Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse.

Appl Microsc 2020 Jun 3;50(1):11. Epub 2020 Jun 3.

Department of Anatomy, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, South Korea.

The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration. Read More

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Live Animal Imaging and Cell Sorting Methods for Investigating Neurodegeneration in a C. elegans Excitotoxic Necrosis Model.

J Vis Exp 2021 01 22(167). Epub 2021 Jan 22.

Cellular, Molecular, and Biomedical Science, CUNY School of Medicine, The City University of New York (CUNY); PhD Program in Biology, The CUNY Graduate Center, The City University of New York (CUNY);

Excitotoxic necrosis is a leading form of neurodegeneration. This process of regulated necrosis is triggered by the synaptic accumulation of the neurotransmitter glutamate, and the excessive stimulation of its postsynaptic receptors. However, information on the subsequent molecular events that culminate in the distinct neuronal swelling morphology of this type of neurodegeneration is lacking. Read More

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January 2021

Measuring collagen fibril diameter with differential interference contrast microscopy.

J Struct Biol 2021 03 2;213(1):107697. Epub 2021 Feb 2.

Department of Bioengineering, Northeastern University, Boston, MA 02115, USA. Electronic address:

Collagen fibrils, linear arrangements of collagen monomers, 20-500 nm in diameter, comprising hundreds of molecules in their cross-section, are the fundamental structural unit in a variety of load-bearing tissues such as tendons, ligaments, skin, cornea, and bone. These fibrils often assemble into more complex structures, providing mechanical stability, strength, or toughness to the host tissue. Unfortunately, there is little information available on individual fibril dynamics, mechanics, growth, aggregation and remodeling because they are difficult to image using visible light as a probe. Read More

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Individual Plasmonic Nanoprobes for Biosensing and Bioimaging: Recent Advances and Perspectives.

Small 2021 Feb 1;17(8):e2004287. Epub 2021 Feb 1.

Department of Vasculocardiology, The Affiliated Hospital of Southwest Medical University, Key Laboratory of Medical Electrophysiology, Ministry of Education, Southwest Medical University, Luzhou, Sichuan, 646000, China.

With the advent of nanofabrication techniques, plasmonic nanoparticles (PNPs) have been widely applied in various research fields ranging from photocatalysis to chemical and bio-sensing. PNPs efficiently convert chemical or physical stimuli in their local environment into optical signals. PNPs also have excellent properties, including good biocompatibility, large surfaces for the attachment of biomolecules, tunable optical properties, strong and stable scattering light, and good conductivity. Read More

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February 2021

Antifibrotic drugs in connective tissue disease-related interstitial lung disease (CTD-ILD): from mechanistic insights to therapeutic applications.

Drugs Context 2021 15;10. Epub 2021 Jan 15.

Discipline of Clinical Pharmacology, College of Medicine and Public Health, Flinders University and Flinders Medical Centre, Adelaide, Australia.

Fibrosing interstitial lung disease (ILD) is one of the most important causes of morbidity and mortality in patients with connective tissue diseases (CTDs), which include systemic sclerosis, rheumatoid arthritis, Sjögren's syndrome, idiopathic inflammatory myositis and systemic lupus erythematosus. The treatment of CTD-ILDs is challenging due to the paucity of proven effective treatments. Recently, two antifibrotic drugs conditionally approved for use in patients with idiopathic pulmonary fibrosis, nintedanib and pirfenidone, have been trialled in CTD-ILDs based on overlapping pathological and clinical features between the two diseases. Read More

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January 2021

Catechin synergistically potentiates mast cell-stabilizing property of caffeine.

Allergy Asthma Clin Immunol 2021 Jan 6;17(1). Epub 2021 Jan 6.

Miyagi University, School of Nursing, 1-1 Gakuen, Taiwa-cho, Kurokawa-gun, Miyagi, 981-3298, Japan.

Caffeine and catechin, contained in coffee and tea, are commonly consumed substances worldwide. Studies revealed their health promoting functions, such as anti-oxidant, anti-cancer and anti-bacterial properties. Additionally, studies also revealed their roles in ameliorating the symptoms of allergic disorders, indicating their anti-allergic properties. Read More

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January 2021

Single-Nanoparticle Orientation Sensing by Deep Learning.

ACS Cent Sci 2020 Dec 9;6(12):2339-2346. Epub 2020 Nov 9.

Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States.

This paper describes a computational imaging platform to determine the orientation of anisotropic optical probes under differential interference contrast (DIC) microscopy. We established a deep-learning model based on data sets of DIC images collected from metal nanoparticle optical probes at different orientations. This model predicted the in-plane angle of gold nanorods with an error below 20°, the inherent limit of the DIC method. Read More

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December 2020

Transcriptomic and Morphological Analysis of Cells Derived from Porcine Buccal Mucosa-Studies on an In Vitro Model.

Animals (Basel) 2020 Dec 24;11(1). Epub 2020 Dec 24.

Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland.

Transcriptional analysis and live-cell imaging are a powerful tool to investigate the dynamics of complex biological systems. In vitro expanded porcine oral mucosal cells, consisting of populations of epithelial and connective lineages, are interesting and complex systems for study via microarray transcriptomic assays to analyze gene expression profile. The transcriptomic analysis included 56 ontological groups with particular focus on 7 gene ontology groups that are related to the processes of differentiation and development. Read More

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December 2020

Quantifying Intracellular Particle Flows by DIC Object Tracking.

Biophys J 2021 02 11;120(3):393-401. Epub 2021 Jan 11.

Division of Biology, Indian Institute of Science Education and Research, Pune, Pashan, Pune, Maharashtra, India. Electronic address:

Label-free imaging techniques such as differential interference contrast (DIC) allow the observation of cells and large subcellular structures in their native, unperturbed states with minimal exposure to light. The development of robust computational image-analysis routines is vital to quantitative label-free imaging. The reliability of quantitative analysis of time-series microscopy data based on single-particle tracking relies on accurately detecting objects as distinct from the background, i. Read More

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February 2021

Minimizing scattering-induced phase errors in differential interference contrast microscopy.

J Biomed Opt 2020 12;25(12)

Utsunomiya University, Department of Optical Engineering, Tochigi, Japan.

Significance: Differential interference contrast (DIC) microscopes allow noninvasive in vivo observation of transparent microstructures in tissue without the use of fluorescent dyes or genetic modification. We show how to modify a DIC microscope to measure the sample phase distribution accurately and in real-time even deep inside sample tissue.

Aim: Our aim is to improve the DIC microscope's phase measurement to remove the phase bias that occurs in the presence of strong scattering. Read More

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December 2020

D-Dimer, Fibrinogen, and IL-6 in COVID-19 Patients with Suspected Venous Thromboembolism: A Narrative Review.

Vasc Health Risk Manag 2020 13;16:455-462. Epub 2020 Nov 13.

Hamad Medical Corporation, Hamad General Hospital, Department of Medicine, Doha, Qatar.

Coronavirus disease 2019 (COVID-19) emerged from the West District of Southern China Seafood Wholesale Market in late December 2019 and has been declared a global pandemic by the World Health Organization (WHO). Infection with severe acute respiratory syndrome coronavirus (SARS-CoV-2) presents with upper respiratory symptoms like cough, fever, and lethargy. At the same time, in later stages, critical COVID-19 patients develop acute respiratory distress syndrome (ARDS), venous thromboembolism (VTE), and multiple organ failure from cytokine storm and coagulation hyperactivity. Read More

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December 2020

The effect of different interference fits on the primary fixation of a cementless femoral component during experimental testing.

J Mech Behav Biomed Mater 2021 01 31;113:104189. Epub 2020 Oct 31.

Radboud University Medical Center, Radboud Institute for Health Sciences, Orthopaedic Research Lab, Nijmegen, the Netherlands.

Cementless femoral total knee arthroplasty (TKA) components use a press-fit (referred to as interference fit) to achieve initial fixation. A higher interference fit could lead to a superior fixation, but it could also introduce more damage to the bone during implantation. The purpose of the current study was to investigate the effect of interference fit on the micromotions and gap opening/closing at the bone-implant interface. Read More

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January 2021

Morphological and physicochemical diversity of snow algae from Alaska.

Sci Rep 2020 11 5;10(1):19167. Epub 2020 Nov 5.

Department of Immunobiology, Institute of Biology Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland.

Snow algae are photosynthetic microbes growing in thawing snow. They usually show various morphological cell types. The aim of this study was to carry out microscopic and spectroscopic analysis of different forms of cells of snow algae collected on glaciers in Alaska. Read More

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November 2020

[A Case of DIC Following Mixed Acidosis during Laparoscopic Hepatectomy].

Gan To Kagaku Ryoho 2020 Sep;47(9):1379-1381

Dept. of Surgery, Gastroenterological Center, Saiseikai Yokohamashi Tobu Hospital.

The patient was an 81-year-old man. Laparoscopic sigmoidectomy was performed for a sigmoid colon cancer(S, type 2, pT3[SS], INF a, Ly0, V0, BD1, Pn0, pPM0, pDM0, RM0, pN0, pM0, pStage Ⅲa, R0, Cur A). One year after the surgery, a blood test showed CEA as high as 68. Read More

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September 2020

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development using Nomarski Microscopy.

J Vis Exp 2020 10 8(164). Epub 2020 Oct 8.

CIBIR (Center for Biomedical Research of La Rioja), La Rioja, Spain;

4D microscopy is an invaluable tool for unraveling the embryonic developmental process in different animals. Over the last decades, Caenorhabditis elegans has emerged as one of the best models for studying development. From an optical point of view, its size and transparent body make this nematode an ideal specimen for DIC (Differential Interference Contrast or Nomarski) microscopy. Read More

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October 2020